CaUC09G163850 (gene) Watermelon (USVL246-FR2) v1

Overview
NameCaUC09G163850
Typegene
OrganismCitrullus amarus (Watermelon (USVL246-FR2) v1)
Descriptionwee1-like protein kinase
LocationCiama_Chr09: 5480930 .. 5485700 (+)
RNA-Seq ExpressionCaUC09G163850
SyntenyCaUC09G163850
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GCAGAGCGTGGGAGGGAAAGCTTCAGCATAGATTCTTTTGATGAGGAGAAAATCCAAAACCCTAAGAACGACGATGACGACGAGGAGGAAGAAGATGAAAGGGACATTGACGAGGCACTTGCAGGTTCAGTTAGGGCAAGTTTCACTCGCTCCTCTCCACCTCCACCGCTCCTCTTCCGCCGCCGCTGCTTGCTTCCAGGACTTGCCTGAATCTGAAGCACCGCCGACGGTCAACGTTTCTGACCCTCTCCCCTCCTCCTCTCCTAACTTTGAGGACAGAGATTTCATTCTTAGCCAGGACTTCTTCTGGTGAGTTTTGTATCGTTCCTTTTTATTTTATTTATTTATTTATTTATTTTTCCCTCCTTTTTACCATTTGTGAGTTGGTTTGAGCTTCTGTTGATGGATTTGAAGGGATTAGTTGGTTGTTCTTTGTTATCTTTCTCAACGTGTTATGAGATGGAGGACTTTTAAACTAACAGAGGTTTTGATCACTTTTGCTAACGTGATAATTAATTTTAGTTTCTGTTAATGGAAGTCATTTCGTAGTAAATTTAATAGACAGAACAATGTTGTGTGATTTTCTGATCATTGACTACTTTTAGAAATTACTTTTGTTTGTCCGTGTTGAGATTGGTATACATGGATGCTAAATGGTAGCTGGTGAGATTGAAGGCTGCATTGTAACTGACCAGATAATTCTTGTGTTGTTTGTTGCAGCACCCCTGACTATATCACCCCGGACAATCAAAATTTGTTGAATGGTGTGGATAACAACGAGGTGAGAAGTTAATGGAGAAAGCTAACCAGGCCAAACACAACGGTTTTTTTCAGAAGAAAAGGCTAATTCTCTAATTTTTTCTGGGTGTCCTTGTCTTGGCAGGAATGCATTCCTTGCCCAAAATCTCCAGAGAAGATTAATACTGTGAAAACTAAGAGAAAAATACATGGTAAGTTGATGATCTCGAACTTCTATCCCAGTTAATAAAGTGTATTGAGCATGACTGATATAAGAGATGAGATACATGCAAATGGCATACACCTAATCTAAGAAGCACAAATACGGAAACGACACAACATGGACACTGCGACACGTCATTTCTTAATAATTTAGACACGACAAAGATACATTTATTTTAATGTACATTTTTAGAAATATATTTCATTCTTTACCGGAAGAAAATCCGAAGTAGATGGGTTTATGCTAAAGCATTTATATGCTTTAAAAATGTCTTTTTAGTCTACACAACAAGTGTTCTATGCGTGTCTAACACATTTGTTGCACTAACAAGTTTCCGATATGTATCCAACAAGTATTTGAATATCCATGTCGGACATGGACACACTAGCCAAACTAAAGTGTCTATGCTTCTTAATGCGTAATTGCAATCAGGTTGAAGCTGAACTTTCCAAATCAATAATGTACATGGTTAATCACTTGCAACTAATGATGATTAGAGATGGAGTCATATCAATTTGGGGACATTGATCTGAAATATTTTTGGGGCTTCAAAATCTGACAGCCTTGTATTCCTTTTTGAAATTAGTGATGTAAGTTGTTCGAATGATGTAATTTAATCATTTTCCATTTAATATTCATTTTGGTGCAGAAAGTGTTTTAGTACATCCTCTGAGTCCGAGTTTGTCTGGCTGCGAACAAGTTGTGGAGCTTGGAAATGACATGTTTGGATCAGATGATGTGGATATGGAGAAAGAGACAATGGCCATGCCAAAGAAAGGAAACTATGTTTCTCAATCTGCAGTTGCTCTACGCTGTCGAGTTATGCCTCCTCCTTGCATGAAGAACCCATACCTGAAAGATGCTTCAGATGTAGGCGTAGACCCATTTGGTAATCAACGAACAAAATGTGCAGGTACAGTGCAATTAGTTCTCAAAGACATCGCAAGTAATTATACGTTTTTTGAAAGCGTCTTTTGCAACGATGATAGATTTCTGTTAGATGAATTAGTTTCGTGGTTTTCAATTTTGTGTAGGTTTTTTTCCTGCTCTGTTTAGTGGAGATGGTCTTTCAAGATATCACACTGATTTTCATGAGATAAAGGTATCCAGAAACATATGCTTAATGCCTACAAGCTTCTGCTGATAGAGATTGGGATTTTATTTTTTATTTTTAATTCTTATTCGATTCAGGCTGTCAAACTTTGCAATAACTTCAGTCTATTTTCAGCAAATAGGGACTGGAAATTTTAGCCGTGTCTTCAAGGTATTAAAGAGAATTGACGGTTGCTTATATGCTGTCAAACAAAGCACAAGACCGTTGAATCAAGACACAGAAAGGTTACTTCTTTTGGTGTGTATCTGTATATGATTTACCTCTAAACACTAGCAAATGTGTAATTGAATTTTTCCCTTCCTGCTGCAGGAGGAGAGCTTTGATGGAAGTTCAAGCTTTGGCAGCTTTAGGTTAGTAAATTTATCTAGATATGGTTTTCCGTTCTATTATATGTATATATATTCCTTTTTCTCTTCAGAGCAATCTTTTTAAAAATTTTTTTTCTTGATTGTTGTCCTGTATTGGAATGTGCAGGATCTCATGAAAACATCGTTGGATACTACTCTTCATGGTTTGAAAATGAACAACTCTATATTCAAATGGAGCTCTGTGATTGTAGTTTATCAATTGGCAGGTATTCCCACCCATTCTCTGAGGTAGATGCGCTGAGAGCCTTGTATCAGGTGAGTTTTAGTTCCATCTTGAATTGTGAAACATTACATTTTCTGCTTACGAAACTTCATTTCCATAAATATATCTTCCGTTAACATATGAAAGAAAGCTTAATTTTGATTTGTTACTTGGTGGGGTAAATTTTTTTCTTCTGCAATTCATTCAATACTGTTAATTTTAACAGGAAATGCCTCATCCGAAAAATAATGATCCAAAATGTTATAAATTTTGTATTAATGTTTTATCTTTATCCATTCCAATTCTTATGTAGCCCTAGTATTTAACATAGAAGCTTTATCCATTCCAATTTTCAAGTTCTAGAACTGGTAACTTATGAAATTTCATGGCTTCATTGCTAACGATGGAACTACAAGCAGATTGCAAAGGCATTGCTATTTGTGCACGAGAAAGGAGTAGCACATTTAGATGTGAAACCTGACAATATTTATGTCAAAGATGGTGTCTATAAACTTGGTGATTTTGGGTGTGTTACCCTTCTTGATAAGAGCCTACCGATTGAGGAGGGTGATGCACGCTATATGCCTCAAGAAATTCTGAATGAAAGATATGATTATCTTGACAAAGTTGATATTTTCTCCTTGGGAGCTGCTATTTATGAAATTGCCCGAGGATCCACATTACCAGAAACGCATTTTATGAACCTTAAGGAGGGAAAATTGCCACTCCTTCCTGGTCATTCATTGCAATTTCAAAACTTAATCAAGGTACTGATTACTATGTCATATTTTGGTTTTCTCTTCATTTCTACTGACTATGCCTTGGGCCTGATTTTAGGCCTTGACTCATCCTCCTTGGATGATGAACCTTGTGGTAGAATCTGGCCATTGTGTACTTACCCATGTTTGCATTTCTTCCCACTAATTTTACTTTCCTTTAAAAACGTACAAAGTTATCATTATCAGAAGGTCAAAACTTATGCCACCAAAAGTAAACCTGAACTAAGTTGCATCCCTACCTTCCAAAAGATAAAAGGAAGCTGCACTTATTTTTTCCCTTAGAAGTTAGAAGATAATTATGTTAACCTATGTTTGCCAAAAGGAATGTTTGATTTTGTTTGTTTAGACCTTCTGAATATAAAATGTAGTGGTCTTTTAGAAAGATGAATGTGATAAATAAAACCAGGCATATCCTTTGAGAATAAACTAGTTTTAGATATTCTCTTAGCTATCTATTCTACATTTGTATAGTTTGAGCTGATTGCTTTCGTACATTCTTTTATTGGTAGAAATGTTGTTTGAGAAGCCTGATTTTAACATTCCAGCAACATAATTCATCAAACAAAAATCCCTAAGATGTATTTGACAATGAGACTAATCTATCCACTCTATAAGGAGTTTAAGGTCTCTTTATTTTTTGAACGTGGAAACCTCAATATACCCTCTCAATATGGTGGCTTTTTGGGTTCACCATTCTTGAATTGAATTTTTTGTTTGAACCAAATGTCTATCTGATATCATATTTTAGATAAACATAGATTTTTATCTCAAACCCAATTGGTAATGAGAGAAATAACCATTTATTTTATAAAAAGTGAGATCTCTTCACTTTTTTTAATGTGAAATTCTCAACACTCTTTCAAGTCTTTTAACAATAAACTTAATAATTTAAAATCTTAACTAGCTTTTGAGTGGATGAAGTCAATAAGGAAATGTTCACTATTTACTTCATTATAGATGTCCAGGACCTTGCAATTTCTTAGCTACTTTTTCTGATTGATTGATAACTGTAACAGGCTATGGTAGATCCTGACCCGACACGGCGTCCTTCGGCAAGGGAAGTTCTTGAGAATCCAATATTTGATAAGGTCAGAAATCATAAACAATCATAGGACAACGTCAACCATGGCGTCTTCTTATTGTTTCACCATTTTTAGAGATGGGCTTTGCTTGTACAATCCCTTGATATCAGCTTGCTATTTTGTAAGATTTCATTCTCCACTTTCCCAAGGTTCATGCACCAAATTTTGGCTATAGCATGGTGCAAGTTTTGTAAATTCTCTATCATGTATTATAAACTCGTAAATGCAAGTTAATGAAAGCTCTTTCATTTTCAAATATTTGACA

mRNA sequence

GCAGAGCGTGGGAGGGAAAGCTTCAGCATAGATTCTTTTGATGAGGAGAAAATCCAAAACCCTAAGAACGACGATGACGACGAGGAGGAAGAAGATGAAAGGGACATTGACGAGGCACTTGCAGGTTCAGTTAGGGCAAGTTTCACTCGCTCCTCTCCACCTCCACCGCTCCTCTTCCGCCGCCGCTGCTTGCTTCCAGGACTTGCCTGAATCTGAAGCACCGCCGACGGTCAACGTTTCTGACCCTCTCCCCTCCTCCTCTCCTAACTTTGAGGACAGAGATTTCATTCTTAGCCAGGACTTCTTCTGCACCCCTGACTATATCACCCCGGACAATCAAAATTTGTTGAATGGTGTGGATAACAACGAGGAATGCATTCCTTGCCCAAAATCTCCAGAGAAGATTAATACTGTGAAAACTAAGAGAAAAATACATGAAAGTGTTTTAGTACATCCTCTGAGTCCGAGTTTGTCTGGCTGCGAACAAGTTGTGGAGCTTGGAAATGACATGTTTGGATCAGATGATGTGGATATGGAGAAAGAGACAATGGCCATGCCAAAGAAAGGAAACTATGTTTCTCAATCTGCAGTTGCTCTACGCTGTCGAGTTATGCCTCCTCCTTGCATGAAGAACCCATACCTGAAAGATGCTTCAGATGTAGGCGTAGACCCATTTGGTAATCAACGAACAAAATGTGCAGGTACAGTGCAATTAGTTCTCAAAGACATCGCAAGTTTTTTTCCTGCTCTGTTTAGTGGAGATGGTCTTTCAAGATATCACACTGATTTTCATGAGATAAAGCAAATAGGGACTGGAAATTTTAGCCGTGTCTTCAAGGTATTAAAGAGAATTGACGGTTGCTTATATGCTGTCAAACAAAGCACAAGACCGTTGAATCAAGACACAGAAAGGAGGAGAGCTTTGATGGAAGTTCAAGCTTTGGCAGCTTTAGGATCTCATGAAAACATCGTTGGATACTACTCTTCATGGTTTGAAAATGAACAACTCTATATTCAAATGGAGCTCTGTGATTGTAGTTTATCAATTGGCAGGTATTCCCACCCATTCTCTGAGGTAGATGCGCTGAGAGCCTTGTATCAGATTGCAAAGGCATTGCTATTTGTGCACGAGAAAGGAGTAGCACATTTAGATGTGAAACCTGACAATATTTATGTCAAAGATGGTGTCTATAAACTTGGTGATTTTGGGTGTGTTACCCTTCTTGATAAGAGCCTACCGATTGAGGAGGGTGATGCACGCTATATGCCTCAAGAAATTCTGAATGAAAGATATGATTATCTTGACAAAGTTGATATTTTCTCCTTGGGAGCTGCTATTTATGAAATTGCCCGAGGATCCACATTACCAGAAACGCATTTTATGAACCTTAAGGAGGGAAAATTGCCACTCCTTCCTGGTCATTCATTGCAATTTCAAAACTTAATCAAGGCTATGGTAGATCCTGACCCGACACGGCGTCCTTCGGCAAGGGAAGTTCTTGAGAATCCAATATTTGATAAGGTCAGAAATCATAAACAATCATAGGACAACGTCAACCATGGCGTCTTCTTATTGTTTCACCATTTTTAGAGATGGGCTTTGCTTGTACAATCCCTTGATATCAGCTTGCTATTTTGTAAGATTTCATTCTCCACTTTCCCAAGGTTCATGCACCAAATTTTGGCTATAGCATGGTGCAAGTTTTGTAAATTCTCTATCATGTATTATAAACTCGTAAATGCAAGTTAATGAAAGCTCTTTCATTTTCAAATATTTGACA

Coding sequence (CDS)

ATGAGGAGAAAATCCAAAACCCTAAGAACGACGATGACGACGAGGAGGAAGAAGATGAAAGGGACATTGACGAGGCACTTGCAGGTTCAGTTAGGGCAAGTTTCACTCGCTCCTCTCCACCTCCACCGCTCCTCTTCCGCCGCCGCTGCTTGCTTCCAGGACTTGCCTGAATCTGAAGCACCGCCGACGGTCAACGTTTCTGACCCTCTCCCCTCCTCCTCTCCTAACTTTGAGGACAGAGATTTCATTCTTAGCCAGGACTTCTTCTGCACCCCTGACTATATCACCCCGGACAATCAAAATTTGTTGAATGGTGTGGATAACAACGAGGAATGCATTCCTTGCCCAAAATCTCCAGAGAAGATTAATACTGTGAAAACTAAGAGAAAAATACATGAAAGTGTTTTAGTACATCCTCTGAGTCCGAGTTTGTCTGGCTGCGAACAAGTTGTGGAGCTTGGAAATGACATGTTTGGATCAGATGATGTGGATATGGAGAAAGAGACAATGGCCATGCCAAAGAAAGGAAACTATGTTTCTCAATCTGCAGTTGCTCTACGCTGTCGAGTTATGCCTCCTCCTTGCATGAAGAACCCATACCTGAAAGATGCTTCAGATGTAGGCGTAGACCCATTTGGTAATCAACGAACAAAATGTGCAGGTACAGTGCAATTAGTTCTCAAAGACATCGCAAGTTTTTTTCCTGCTCTGTTTAGTGGAGATGGTCTTTCAAGATATCACACTGATTTTCATGAGATAAAGCAAATAGGGACTGGAAATTTTAGCCGTGTCTTCAAGGTATTAAAGAGAATTGACGGTTGCTTATATGCTGTCAAACAAAGCACAAGACCGTTGAATCAAGACACAGAAAGGAGGAGAGCTTTGATGGAAGTTCAAGCTTTGGCAGCTTTAGGATCTCATGAAAACATCGTTGGATACTACTCTTCATGGTTTGAAAATGAACAACTCTATATTCAAATGGAGCTCTGTGATTGTAGTTTATCAATTGGCAGGTATTCCCACCCATTCTCTGAGGTAGATGCGCTGAGAGCCTTGTATCAGATTGCAAAGGCATTGCTATTTGTGCACGAGAAAGGAGTAGCACATTTAGATGTGAAACCTGACAATATTTATGTCAAAGATGGTGTCTATAAACTTGGTGATTTTGGGTGTGTTACCCTTCTTGATAAGAGCCTACCGATTGAGGAGGGTGATGCACGCTATATGCCTCAAGAAATTCTGAATGAAAGATATGATTATCTTGACAAAGTTGATATTTTCTCCTTGGGAGCTGCTATTTATGAAATTGCCCGAGGATCCACATTACCAGAAACGCATTTTATGAACCTTAAGGAGGGAAAATTGCCACTCCTTCCTGGTCATTCATTGCAATTTCAAAACTTAATCAAGGCTATGGTAGATCCTGACCCGACACGGCGTCCTTCGGCAAGGGAAGTTCTTGAGAATCCAATATTTGATAAGGTCAGAAATCATAAACAATCATAG

Protein sequence

MRRKSKTLRTTMTTRRKKMKGTLTRHLQVQLGQVSLAPLHLHRSSSAAAACFQDLPESEAPPTVNVSDPLPSSSPNFEDRDFILSQDFFCTPDYITPDNQNLLNGVDNNEECIPCPKSPEKINTVKTKRKIHESVLVHPLSPSLSGCEQVVELGNDMFGSDDVDMEKETMAMPKKGNYVSQSAVALRCRVMPPPCMKNPYLKDASDVGVDPFGNQRTKCAGTVQLVLKDIASFFPALFSGDGLSRYHTDFHEIKQIGTGNFSRVFKVLKRIDGCLYAVKQSTRPLNQDTERRRALMEVQALAALGSHENIVGYYSSWFENEQLYIQMELCDCSLSIGRYSHPFSEVDALRALYQIAKALLFVHEKGVAHLDVKPDNIYVKDGVYKLGDFGCVTLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLGAAIYEIARGSTLPETHFMNLKEGKLPLLPGHSLQFQNLIKAMVDPDPTRRPSAREVLENPIFDKVRNHKQS
Homology
BLAST of CaUC09G163850 vs. NCBI nr
Match: XP_038876752.1 (wee1-like protein kinase [Benincasa hispida])

HSP 1 Score: 951.8 bits (2459), Expect = 2.3e-273
Identity = 474/503 (94.23%), Postives = 479/503 (95.23%), Query Frame = 0

Query: 1   MRRKSKTLRTTMTTRRKKMKGTLTRHLQVQLGQVSLAPLHLHRSSSAA----AACFQDLP 60
           MRRKS+TLRTTMTTRRKKMKGTLTRHLQVQLGQVSLAPLHLHRSSS+A    AACFQDLP
Sbjct: 1   MRRKSRTLRTTMTTRRKKMKGTLTRHLQVQLGQVSLAPLHLHRSSSSAAASTAACFQDLP 60

Query: 61  ESEAPPTVNVSDPLPSSSPNFEDRDFILSQDFFCTPDYITPDNQNLLNGVDNNEECIPCP 120
           ESEAPPTVN SD LPS SPNFEDRDFILSQDFFCTPDYITPDNQNLLNGVD+N+ECIPCP
Sbjct: 61  ESEAPPTVNASDALPSPSPNFEDRDFILSQDFFCTPDYITPDNQNLLNGVDSNQECIPCP 120

Query: 121 KSPEKINTVKTKRKIHESVLVHPLSPSLSGCEQVVELGNDMFGSDDVDMEKETMAMPKKG 180
           KSPEKINTVKTKRKIHESVLVHPLSPSLSGCEQVVELGND FGSDDVDMEKETMAMPKK 
Sbjct: 121 KSPEKINTVKTKRKIHESVLVHPLSPSLSGCEQVVELGNDTFGSDDVDMEKETMAMPKKQ 180

Query: 181 NYVSQSAVALRCRVMPPPCMKNPYLKDASDVGVDPFGNQRTKCAGTVQLVLKDIASFFPA 240
           NYVSQSAVALRCRVMPPPCMKNPYLKDASDVGVDPFGNQRTKCAG           FFPA
Sbjct: 181 NYVSQSAVALRCRVMPPPCMKNPYLKDASDVGVDPFGNQRTKCAG-----------FFPA 240

Query: 241 LFSGDGLSRYHTDFHEIKQIGTGNFSRVFKVLKRIDGCLYAVKQSTRPLNQDTERRRALM 300
           LFSGDGLSRYHTDFHEIKQIGTGNFSRVFKVLKRIDGCLYAVKQSTRPLNQD ERRRALM
Sbjct: 241 LFSGDGLSRYHTDFHEIKQIGTGNFSRVFKVLKRIDGCLYAVKQSTRPLNQDIERRRALM 300

Query: 301 EVQALAALGSHENIVGYYSSWFENEQLYIQMELCDCSLSIGRYSHPFSEVDALRALYQIA 360
           EVQALAALGSHENIVGYYSSWFENEQLYIQMELCDCSLSIGRYSHPF EVDALRALYQIA
Sbjct: 301 EVQALAALGSHENIVGYYSSWFENEQLYIQMELCDCSLSIGRYSHPFFEVDALRALYQIA 360

Query: 361 KALLFVHEKGVAHLDVKPDNIYVKDGVYKLGDFGCVTLLDKSLPIEEGDARYMPQEILNE 420
           KALLFVHEKGVAHLDVKPDNIYVKDGV+KLGDFGCVTLLDKSLPIEEGDARYMPQEILNE
Sbjct: 361 KALLFVHEKGVAHLDVKPDNIYVKDGVFKLGDFGCVTLLDKSLPIEEGDARYMPQEILNE 420

Query: 421 RYDYLDKVDIFSLGAAIYEIARGSTLPETHFMNLKEGKLPLLPGHSLQFQNLIKAMVDPD 480
           RYDYLDKVDIFSLGAAIYEI RGSTLPE HFMNLKEGKLPLLPGHSLQFQNLIKAMVDPD
Sbjct: 421 RYDYLDKVDIFSLGAAIYEIVRGSTLPEMHFMNLKEGKLPLLPGHSLQFQNLIKAMVDPD 480

Query: 481 PTRRPSAREVLENPIFDKVRNHK 500
           PTRRPSAREVLENPIFDKVRNHK
Sbjct: 481 PTRRPSAREVLENPIFDKVRNHK 492

BLAST of CaUC09G163850 vs. NCBI nr
Match: XP_008451611.1 (PREDICTED: wee1-like protein kinase [Cucumis melo])

HSP 1 Score: 927.5 bits (2396), Expect = 4.7e-266
Identity = 462/501 (92.22%), Postives = 475/501 (94.81%), Query Frame = 0

Query: 1   MRRKSKTLRTTMTTRRKKMKGTLTRHLQVQLGQVSLAPLHLHRSSSAAAACFQDLPESEA 60
           MRRKSKTLRTTM TRRKKMKGT+TRHLQVQLG+VSLAPL LHRSSS +AACFQ+LPESEA
Sbjct: 1   MRRKSKTLRTTMGTRRKKMKGTVTRHLQVQLGKVSLAPLQLHRSSS-SAACFQNLPESEA 60

Query: 61  PPTVNVSDPLPSSSPNFEDRDFILSQDFFCTPDYITPDNQNLLNGVDNNEECIPCPKSPE 120
           P TVNVSD LPSSS NFEDRDFILSQDFFCTPDYITPDNQNLLNGVD+N+ECIPCPKSPE
Sbjct: 61  PSTVNVSDALPSSSSNFEDRDFILSQDFFCTPDYITPDNQNLLNGVDSNKECIPCPKSPE 120

Query: 121 KINTVKTKRKIHESVLVHPLSPSLSGCEQVVELGNDMFGSDDVDMEKETMAMPKKGNYVS 180
           KINTVKTKRKIHE+VLV PLSPSLSGCEQVVELGND FGSDDVDMEKETMAMPKK +YVS
Sbjct: 121 KINTVKTKRKIHENVLVRPLSPSLSGCEQVVELGNDTFGSDDVDMEKETMAMPKKESYVS 180

Query: 181 QSAVALRCRVMPPPCMKNPYLKDASDVGVDPFGNQRTKCAGTVQLVLKDIASFFPALFSG 240
           QSAVALRCRVMPPPCMKNPYL DASDVGVDPFGNQRTKCAG           FFPALFSG
Sbjct: 181 QSAVALRCRVMPPPCMKNPYLGDASDVGVDPFGNQRTKCAG-----------FFPALFSG 240

Query: 241 DGLSRYHTDFHEIKQIGTGNFSRVFKVLKRIDGCLYAVKQSTRPLNQDTERRRALMEVQA 300
           DGLSRYHTDFHEIK IGTGNFSRVFKVLKRIDGCLYAVKQSTRPLNQDTERRRALMEVQA
Sbjct: 241 DGLSRYHTDFHEIKLIGTGNFSRVFKVLKRIDGCLYAVKQSTRPLNQDTERRRALMEVQA 300

Query: 301 LAALGSHENIVGYYSSWFENEQLYIQMELCDCSLSIGRYSHPFSEVDALRALYQIAKALL 360
           LAALGSHENIVGYY+SWFENEQLYIQMELCDCSLS+G YSHPFSEVDALRALYQIAKALL
Sbjct: 301 LAALGSHENIVGYYTSWFENEQLYIQMELCDCSLSMGTYSHPFSEVDALRALYQIAKALL 360

Query: 361 FVHEKGVAHLDVKPDNIYVKDGVYKLGDFGCVTLLDKSLPIEEGDARYMPQEILNERYDY 420
           FVHEKG+AHLDVKPDNIY+KDGVYKLGDFGCVTLLDKSLPIEEGDARYMPQEILNERYDY
Sbjct: 361 FVHEKGIAHLDVKPDNIYIKDGVYKLGDFGCVTLLDKSLPIEEGDARYMPQEILNERYDY 420

Query: 421 LDKVDIFSLGAAIYEIARGSTLPETHFMNLKEGKLPLLPGHSLQFQNLIKAMVDPDPTRR 480
           LDKVDIFSLGAAIYEI RG TLPETHFMNLKEGKLPLLPGHSLQFQNLIKAMVDP+PTRR
Sbjct: 421 LDKVDIFSLGAAIYEIVRGFTLPETHFMNLKEGKLPLLPGHSLQFQNLIKAMVDPNPTRR 480

Query: 481 PSAREVLENPIFDKVRNHKQS 502
           PSAREVLENPIFDKVR HKQS
Sbjct: 481 PSAREVLENPIFDKVRTHKQS 489

BLAST of CaUC09G163850 vs. NCBI nr
Match: XP_004136046.1 (wee1-like protein kinase isoform X2 [Cucumis sativus] >KGN44910.1 hypothetical protein Csa_015648 [Cucumis sativus])

HSP 1 Score: 923.7 bits (2386), Expect = 6.8e-265
Identity = 457/501 (91.22%), Postives = 473/501 (94.41%), Query Frame = 0

Query: 1   MRRKSKTLRTTMTTRRKKMKGTLTRHLQVQLGQVSLAPLHLHRSSSAAAACFQDLPESEA 60
           MRRKSKTLRTTM  RRKKMKGT+TRHLQVQLG+VSLAPLHL+RSSS +A CF  LPESE 
Sbjct: 1   MRRKSKTLRTTMGARRKKMKGTVTRHLQVQLGKVSLAPLHLNRSSS-SALCFPSLPESEP 60

Query: 61  PPTVNVSDPLPSSSPNFEDRDFILSQDFFCTPDYITPDNQNLLNGVDNNEECIPCPKSPE 120
           P TVN SDPLPSSS NFEDRDFILSQDFFCTPDYITPDNQNLLNGVD+N+ECIPCPKSPE
Sbjct: 61  PSTVNFSDPLPSSSHNFEDRDFILSQDFFCTPDYITPDNQNLLNGVDSNKECIPCPKSPE 120

Query: 121 KINTVKTKRKIHESVLVHPLSPSLSGCEQVVELGNDMFGSDDVDMEKETMAMPKKGNYVS 180
           K+NTVKTKRKIHE+VLV PLSPSLSGCEQVVELGND+FGSDDVDMEKET AMPKK NYVS
Sbjct: 121 KVNTVKTKRKIHENVLVRPLSPSLSGCEQVVELGNDIFGSDDVDMEKETTAMPKKENYVS 180

Query: 181 QSAVALRCRVMPPPCMKNPYLKDASDVGVDPFGNQRTKCAGTVQLVLKDIASFFPALFSG 240
           QSAVALRCRVMPPPCMKNPYLKDASDVGVDPFGNQRTKCAG           FFPALFSG
Sbjct: 181 QSAVALRCRVMPPPCMKNPYLKDASDVGVDPFGNQRTKCAG-----------FFPALFSG 240

Query: 241 DGLSRYHTDFHEIKQIGTGNFSRVFKVLKRIDGCLYAVKQSTRPLNQDTERRRALMEVQA 300
           DGLSRYHTDFHEIK IGTGNFSRVFKVLKRIDGCLYAVKQSTRPLNQDTERRRALMEVQA
Sbjct: 241 DGLSRYHTDFHEIKLIGTGNFSRVFKVLKRIDGCLYAVKQSTRPLNQDTERRRALMEVQA 300

Query: 301 LAALGSHENIVGYYSSWFENEQLYIQMELCDCSLSIGRYSHPFSEVDALRALYQIAKALL 360
           LAALG+HENIVGYY+SWFENEQLYIQMELCDCSLS+GRYSHPFSEVDALRALYQIAKALL
Sbjct: 301 LAALGAHENIVGYYTSWFENEQLYIQMELCDCSLSMGRYSHPFSEVDALRALYQIAKALL 360

Query: 361 FVHEKGVAHLDVKPDNIYVKDGVYKLGDFGCVTLLDKSLPIEEGDARYMPQEILNERYDY 420
           FVHEKG+AHLDVKPDNIY+KDGVYKLGDFGCVTLLDKSLPIEEGDARYMPQEILNERYDY
Sbjct: 361 FVHEKGIAHLDVKPDNIYIKDGVYKLGDFGCVTLLDKSLPIEEGDARYMPQEILNERYDY 420

Query: 421 LDKVDIFSLGAAIYEIARGSTLPETHFMNLKEGKLPLLPGHSLQFQNLIKAMVDPDPTRR 480
           LDKVDIFSLGAAIYEI RG TLPETHFMNLKEGKLPLLPGHSLQFQNLIKAMVDPDP+RR
Sbjct: 421 LDKVDIFSLGAAIYEIVRGFTLPETHFMNLKEGKLPLLPGHSLQFQNLIKAMVDPDPSRR 480

Query: 481 PSAREVLENPIFDKVRNHKQS 502
           PSAREV+ENPIFDKVR HKQS
Sbjct: 481 PSAREVIENPIFDKVRTHKQS 489

BLAST of CaUC09G163850 vs. NCBI nr
Match: XP_031744240.1 (wee1-like protein kinase isoform X1 [Cucumis sativus])

HSP 1 Score: 919.1 bits (2374), Expect = 1.7e-263
Identity = 457/502 (91.04%), Postives = 473/502 (94.22%), Query Frame = 0

Query: 1   MRRKSKTLRTTMTTRRKKMKGTLTRHLQVQLGQVSLAPLHLHRSSSAAAACFQDLPESEA 60
           MRRKSKTLRTTM  RRKKMKGT+TRHLQVQLG+VSLAPLHL+RSSS +A CF  LPESE 
Sbjct: 1   MRRKSKTLRTTMGARRKKMKGTVTRHLQVQLGKVSLAPLHLNRSSS-SALCFPSLPESEP 60

Query: 61  PPTVNVSDPLPSSSPNFEDRDFILSQDFFCTPDYITPDNQNLLNGVDNNEECIPCPKSPE 120
           P TVN SDPLPSSS NFEDRDFILSQDFFCTPDYITPDNQNLLNGVD+N+ECIPCPKSPE
Sbjct: 61  PSTVNFSDPLPSSSHNFEDRDFILSQDFFCTPDYITPDNQNLLNGVDSNKECIPCPKSPE 120

Query: 121 KINTVKTKRKIH-ESVLVHPLSPSLSGCEQVVELGNDMFGSDDVDMEKETMAMPKKGNYV 180
           K+NTVKTKRKIH E+VLV PLSPSLSGCEQVVELGND+FGSDDVDMEKET AMPKK NYV
Sbjct: 121 KVNTVKTKRKIHAENVLVRPLSPSLSGCEQVVELGNDIFGSDDVDMEKETTAMPKKENYV 180

Query: 181 SQSAVALRCRVMPPPCMKNPYLKDASDVGVDPFGNQRTKCAGTVQLVLKDIASFFPALFS 240
           SQSAVALRCRVMPPPCMKNPYLKDASDVGVDPFGNQRTKCAG           FFPALFS
Sbjct: 181 SQSAVALRCRVMPPPCMKNPYLKDASDVGVDPFGNQRTKCAG-----------FFPALFS 240

Query: 241 GDGLSRYHTDFHEIKQIGTGNFSRVFKVLKRIDGCLYAVKQSTRPLNQDTERRRALMEVQ 300
           GDGLSRYHTDFHEIK IGTGNFSRVFKVLKRIDGCLYAVKQSTRPLNQDTERRRALMEVQ
Sbjct: 241 GDGLSRYHTDFHEIKLIGTGNFSRVFKVLKRIDGCLYAVKQSTRPLNQDTERRRALMEVQ 300

Query: 301 ALAALGSHENIVGYYSSWFENEQLYIQMELCDCSLSIGRYSHPFSEVDALRALYQIAKAL 360
           ALAALG+HENIVGYY+SWFENEQLYIQMELCDCSLS+GRYSHPFSEVDALRALYQIAKAL
Sbjct: 301 ALAALGAHENIVGYYTSWFENEQLYIQMELCDCSLSMGRYSHPFSEVDALRALYQIAKAL 360

Query: 361 LFVHEKGVAHLDVKPDNIYVKDGVYKLGDFGCVTLLDKSLPIEEGDARYMPQEILNERYD 420
           LFVHEKG+AHLDVKPDNIY+KDGVYKLGDFGCVTLLDKSLPIEEGDARYMPQEILNERYD
Sbjct: 361 LFVHEKGIAHLDVKPDNIYIKDGVYKLGDFGCVTLLDKSLPIEEGDARYMPQEILNERYD 420

Query: 421 YLDKVDIFSLGAAIYEIARGSTLPETHFMNLKEGKLPLLPGHSLQFQNLIKAMVDPDPTR 480
           YLDKVDIFSLGAAIYEI RG TLPETHFMNLKEGKLPLLPGHSLQFQNLIKAMVDPDP+R
Sbjct: 421 YLDKVDIFSLGAAIYEIVRGFTLPETHFMNLKEGKLPLLPGHSLQFQNLIKAMVDPDPSR 480

Query: 481 RPSAREVLENPIFDKVRNHKQS 502
           RPSAREV+ENPIFDKVR HKQS
Sbjct: 481 RPSAREVIENPIFDKVRTHKQS 490

BLAST of CaUC09G163850 vs. NCBI nr
Match: KAA0068026.1 (wee1-like protein kinase [Cucumis melo var. makuwa])

HSP 1 Score: 910.6 bits (2352), Expect = 5.9e-261
Identity = 451/490 (92.04%), Postives = 464/490 (94.69%), Query Frame = 0

Query: 12  MTTRRKKMKGTLTRHLQVQLGQVSLAPLHLHRSSSAAAACFQDLPESEAPPTVNVSDPLP 71
           M TRRKKMKGT+TRHLQVQLG+VSLAPL LHRSSS +AACFQ+LPESEAP TVNVSD LP
Sbjct: 1   MGTRRKKMKGTVTRHLQVQLGKVSLAPLQLHRSSS-SAACFQNLPESEAPSTVNVSDALP 60

Query: 72  SSSPNFEDRDFILSQDFFCTPDYITPDNQNLLNGVDNNEECIPCPKSPEKINTVKTKRKI 131
           SSS NFEDRDFILSQDFFCTPDYITPDNQNLLNGVD+N+ECIPCPKSPEKINTVKTKRKI
Sbjct: 61  SSSSNFEDRDFILSQDFFCTPDYITPDNQNLLNGVDSNKECIPCPKSPEKINTVKTKRKI 120

Query: 132 HESVLVHPLSPSLSGCEQVVELGNDMFGSDDVDMEKETMAMPKKGNYVSQSAVALRCRVM 191
           HE+VLV PLSPSLSGCEQVVELGND FGSDDVDMEKETMAMPKK +YVSQSAVALRCRVM
Sbjct: 121 HENVLVRPLSPSLSGCEQVVELGNDTFGSDDVDMEKETMAMPKKESYVSQSAVALRCRVM 180

Query: 192 PPPCMKNPYLKDASDVGVDPFGNQRTKCAGTVQLVLKDIASFFPALFSGDGLSRYHTDFH 251
           PPPCMKNPYL DASDVGVDPFGNQRTKCAG           FFPALFSGDGLSRYHTDFH
Sbjct: 181 PPPCMKNPYLGDASDVGVDPFGNQRTKCAG-----------FFPALFSGDGLSRYHTDFH 240

Query: 252 EIKQIGTGNFSRVFKVLKRIDGCLYAVKQSTRPLNQDTERRRALMEVQALAALGSHENIV 311
           EIK IGTGNFSRVFKVLKRIDGCLYAVKQSTRPLNQDTERRRALMEVQALAALGSHENIV
Sbjct: 241 EIKLIGTGNFSRVFKVLKRIDGCLYAVKQSTRPLNQDTERRRALMEVQALAALGSHENIV 300

Query: 312 GYYSSWFENEQLYIQMELCDCSLSIGRYSHPFSEVDALRALYQIAKALLFVHEKGVAHLD 371
           GYY+SWFENEQLYIQMELCDCSLS+G YSHPFSEVDALRALYQIAKALLFVHEKG+AHLD
Sbjct: 301 GYYTSWFENEQLYIQMELCDCSLSMGTYSHPFSEVDALRALYQIAKALLFVHEKGIAHLD 360

Query: 372 VKPDNIYVKDGVYKLGDFGCVTLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLGA 431
           VKPDNIY+KDGVYKLGDFGCVTLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLGA
Sbjct: 361 VKPDNIYIKDGVYKLGDFGCVTLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLGA 420

Query: 432 AIYEIARGSTLPETHFMNLKEGKLPLLPGHSLQFQNLIKAMVDPDPTRRPSAREVLENPI 491
           AIYEI RG TLPETHFMNLKEGKLPLLPGHSLQFQNLIKAMVDP+PTRRPSAREVLENPI
Sbjct: 421 AIYEIVRGFTLPETHFMNLKEGKLPLLPGHSLQFQNLIKAMVDPNPTRRPSAREVLENPI 478

Query: 492 FDKVRNHKQS 502
           FDKVR HKQS
Sbjct: 481 FDKVRTHKQS 478

BLAST of CaUC09G163850 vs. ExPASy Swiss-Prot
Match: Q8L4H0 (Wee1-like protein kinase OS=Arabidopsis thaliana OX=3702 GN=WEE1 PE=1 SV=1)

HSP 1 Score: 569.3 bits (1466), Expect = 4.3e-161
Identity = 295/499 (59.12%), Postives = 369/499 (73.95%), Query Frame = 0

Query: 2   RRKSKTLRTTMTTRRKKMKGTLTRHLQVQLGQVSLAPLHLHRSSSAAAACFQDLPESEAP 61
           R+   T++T  + + +KM+GTL RH  +Q GQ+S        SS+ A++ FQ L +S++ 
Sbjct: 13  RKTQGTIKTRASKKIRKMEGTLERHSLLQFGQLSKISFENRPSSNVASSAFQGLLDSDSS 72

Query: 62  PTVNVSDPLPSSSPNFEDRDFILSQDFFCTPDYITPDNQNLLNGVDNNEECIPCPKSPEK 121
              N      S + N  ++DFILSQDFFCTPDYITPDNQNL++G+D +++  PCP+SP K
Sbjct: 73  ELRNQLGSADSDA-NCGEKDFILSQDFFCTPDYITPDNQNLMSGLDISKDHSPCPRSPVK 132

Query: 122 INTVKTKRKIHESVLVHPLSPSLSGCEQVVELGNDMFGSDDVDMEKETMAMPKKGNYVSQ 181
           +NTVK+KR   ES   +  + + S   +V E  ND   +D+V  +K      ++  YVSQ
Sbjct: 133 LNTVKSKRCRQESFTGNHSNSTWSSKHRVDEQENDDIDTDEVMGDKLQANQTERTGYVSQ 192

Query: 182 SAVALRCRVMPPPCMKNPYLKDASDVGVDPFGNQRTKCAGTVQLVLKDIASFFPALFSGD 241
           +AVALRCR MPPPC+KNPY+ + S+   DPFG+QR+KC           ASF P   SGD
Sbjct: 193 AAVALRCRAMPPPCLKNPYVLNQSETATDPFGHQRSKC-----------ASFLPVSTSGD 252

Query: 242 GLSRYHTDFHEIKQIGTGNFSRVFKVLKRIDGCLYAVKQSTRPLNQDTERRRALMEVQAL 301
           GLSRY TDFHEI+QIG G+FSRVFKVLKR+DGCLYAVK STR L  D+ERR+A+MEVQAL
Sbjct: 253 GLSRYLTDFHEIRQIGAGHFSRVFKVLKRMDGCLYAVKHSTRKLYLDSERRKAMMEVQAL 312

Query: 302 AALGSHENIVGYYSSWFENEQLYIQMELCDCSLSI--GRYSHPFSEVDALRALYQIAKAL 361
           AALG HENIVGYYSSWFENEQLYIQ+ELCD SLS    + S   SE + L  ++QIAKAL
Sbjct: 313 AALGFHENIVGYYSSWFENEQLYIQLELCDHSLSALPKKSSLKVSEREILVIMHQIAKAL 372

Query: 362 LFVHEKGVAHLDVKPDNIYVKDGVYKLGDFGCVTLLDKSLPIEEGDARYMPQEILNERYD 421
            FVHEKG+AHLDVKPDNIY+K+GV KLGDFGC T LDKSLP+EEGDARYMPQEILNE Y+
Sbjct: 373 HFVHEKGIAHLDVKPDNIYIKNGVCKLGDFGCATRLDKSLPVEEGDARYMPQEILNEDYE 432

Query: 422 YLDKVDIFSLGAAIYEIARGSTLPET--HFMNLKEGKLPLLPGHSLQFQNLIKAMVDPDP 481
           +LDKVDIFSLG  +YE+ +GS L E+    +N+KEGKLPLLPGHSLQ Q L+K M+D DP
Sbjct: 433 HLDKVDIFSLGVTVYELIKGSPLTESRNQSLNIKEGKLPLLPGHSLQLQQLLKTMMDRDP 492

Query: 482 TRRPSAREVLENPIFDKVR 497
            RRPSARE+L++P+FD++R
Sbjct: 493 KRRPSARELLDHPMFDRIR 499

BLAST of CaUC09G163850 vs. ExPASy Swiss-Prot
Match: Q6Z829 (Wee1-like protein kinase OS=Oryza sativa subsp. japonica OX=39947 GN=WEE1 PE=3 SV=1)

HSP 1 Score: 490.3 bits (1261), Expect = 2.5e-137
Identity = 269/492 (54.67%), Postives = 338/492 (68.70%), Query Frame = 0

Query: 27  LQVQLGQVSLAPLHLHRSSSAAAAC------FQDLPESEAPPTVNVSDPLPSSSPN---- 86
           L +QL  VSL           AAA       F++L E    P      PLP   P     
Sbjct: 42  LSLQLEHVSLFSFLADAPREGAAAARTPFTPFEELLEGSCDPDPTPPPPLPPLQPQATPM 101

Query: 87  -----FEDRDF-ILSQDFFCTPDYITPDNQNLLNGVDNNEECIPCPKSPEKINTVKTKRK 146
                 E++D  ILSQDFFCTPDYITPD   L +G D N+E IPCP SPEK +  ++KR 
Sbjct: 102 DADEVVEEKDSGILSQDFFCTPDYITPDAPQLGSGFDANKENIPCPNSPEK-SVCRSKRY 161

Query: 147 IHESVLVHPLSPSLSGCEQVVE---LGNDMF-GSDDVDMEK-ETMAMPKKGNYVSQSAVA 206
             +       SP   G   + +   +    F G DD + E+ +  +  K+G+YVSQSAVA
Sbjct: 162 KRD------CSPKGLGSNDIFDSQWIAPVQFEGLDDSEEEQLKESSSHKRGSYVSQSAVA 221

Query: 207 LRCRVMPPPCMKNPYLKDASDVGVDPFGNQRTKCAGTVQLVLKDIASFFPALFSGDGLSR 266
           LRCRVMPPPC++NPYL     +  + FG ++ K +G           F P++  GDGLSR
Sbjct: 222 LRCRVMPPPCIRNPYLNTDHQIDDNVFGGRQCKSSG-----------FSPSV-DGDGLSR 281

Query: 267 YHTDFHEIKQIGTGNFSRVFKVLKRIDGCLYAVKQSTRPLNQDTERRRALMEVQALAALG 326
           Y TDFHEI+QIG GNFS VFKVLKRIDGCLYAVK+S R L+ D ERR+A+ EVQALAALG
Sbjct: 282 YRTDFHEIEQIGRGNFSVVFKVLKRIDGCLYAVKRSIRQLHNDRERRQAVKEVQALAALG 341

Query: 327 SHENIVGYYSSWFENEQLYIQMELCDCSLSIGRYSHPFSEVDALRALYQIAKALLFVHEK 386
            HENIVGY++SWFEN+QL+IQMELCD  LS+ R + P    +AL  LYQI K L F+HE+
Sbjct: 342 CHENIVGYFTSWFENKQLFIQMELCDRCLSMDR-NQPLKCGEALELLYQICKGLDFIHER 401

Query: 387 GVAHLDVKPDNIYVKDGVYKLGDFGCVTLLDKSLPIEEGDARYMPQEILNERYDYLDKVD 446
           G+AHLDVKPDNIYV++GVYKLGDFGC TL+D+SL IE+GD+RYMP E+LN++Y++LDKVD
Sbjct: 402 GIAHLDVKPDNIYVRNGVYKLGDFGCATLIDRSLAIEDGDSRYMPPEMLNDKYEHLDKVD 461

Query: 447 IFSLGAAIYEIARGSTLPET--HFMNLKEGKLPLLPGHSLQFQNLIKAMVDPDPTRRPSA 496
           IFSLGAAIYE+ RG+ LP++   F +L+EGK+ LLPG  +QFQ+LIK+M+DPDP RRPSA
Sbjct: 462 IFSLGAAIYELIRGTQLPDSGPQFTSLREGKIALLPGCPMQFQSLIKSMMDPDPVRRPSA 513

BLAST of CaUC09G163850 vs. ExPASy Swiss-Prot
Match: P54350 (Wee1-like protein kinase OS=Drosophila melanogaster OX=7227 GN=Wee1 PE=1 SV=2)

HSP 1 Score: 200.7 bits (509), Expect = 4.0e-50
Identity = 107/290 (36.90%), Postives = 159/290 (54.83%), Query Frame = 0

Query: 243 LSRYHTDFHEIKQIGTGNFSRVFKVLKRIDGCLYAVKQSTRPLNQDTERRRALMEVQALA 302
           +SR+  +F ++  IG G F  VF+ + R+DGC+YA+K+S +P+   +  +RAL EV A A
Sbjct: 232 ISRFKREFMQVNVIGVGEFGVVFQCVNRLDGCIYAIKKSKKPVAGSSFEKRALNEVWAHA 291

Query: 303 ALGSHENIVGYYSSWFENEQLYIQMELCD-CSLSIGRYSHPFSEVDALRALYQIAKALLF 362
            LG H+N+V YYS+W E++ + IQ E CD  SL      H   E +    L  + + L +
Sbjct: 292 VLGKHDNVVRYYSAWAEDDHMLIQNEFCDGGSLHARIQDHCLGEAELKIVLMHVIEGLRY 351

Query: 363 VHEKGVAHLDVKPDNIY-----------------VKDG-----------------VYKLG 422
           +H   + H+D+KP+NI+                  KD                   YK+G
Sbjct: 352 IHSNDLVHMDLKPENIFSTMNPNAHKLVEVQPQQTKDDDGMDSVYEELRHSENLVTYKIG 411

Query: 423 DFGCVTLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLGAAIYEIARGSTLPET-- 482
           D G VT + K   +EEGD RY+P+EIL+E Y  L K DIFSLG  ++E A G  LP+   
Sbjct: 412 DLGHVTSV-KEPYVEEGDCRYLPKEILHEDYSNLFKADIFSLGITLFEAAGGGPLPKNGP 471

Query: 483 HFMNLKEGKLPLLPGHSLQFQNLIKAMVDPDPTRRPSAREVLENPIFDKV 496
            + NL++GK+P+LP  S  F  LI  M+ P P +RP+++ +  +PI   V
Sbjct: 472 EWHNLRDGKVPILPSLSRDFNELIAQMMHPYPDKRPTSQSIFSHPILSAV 520

BLAST of CaUC09G163850 vs. ExPASy Swiss-Prot
Match: Q1LX51 (Wee1-like protein kinase 2 OS=Danio rerio OX=7955 GN=wee2 PE=2 SV=2)

HSP 1 Score: 198.4 bits (503), Expect = 2.0e-49
Identity = 116/312 (37.18%), Postives = 173/312 (55.45%), Query Frame = 0

Query: 223 VQLVLKDIASFFPA---LFSGDGLSRYHTDFHEIKQIGTGNFSRVFKVLKRIDGCLYAVK 282
           +Q   +D + F P+     S   LSRY ++F E+  IG G F  V++ +KR+DGC+YA+K
Sbjct: 158 IQNSSEDESFFLPSKRPAVSARMLSRYESEFLELACIGVGEFGSVYRCVKRLDGCMYAIK 217

Query: 283 QSTRPLNQDTERRRALMEVQALAALGSHENIVGYYSSWFENEQLYIQMELCD-----CSL 342
           +S RP+      + AL EV A A LG H ++V YYS+W E++ + IQ E CD      ++
Sbjct: 218 RSRRPIAGSANEQLALKEVYAHAVLGHHPHVVRYYSAWAEDDHMIIQNEYCDGGSLHDAI 277

Query: 343 SIGRYSHPFSEVDALR-ALYQIAKALLFVHEKGVAHLDVKPDNIYV-------------- 402
           +  R    F  V  LR  L Q++  L ++H  G+ HLD+KP NI++              
Sbjct: 278 TEKREQGEFFSVPELRDLLLQVSMGLKYIHNSGLVHLDIKPSNIFICRRSTLSAGGEGDS 337

Query: 403 --------KDGVYKLGDFGCVTLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLGA 462
                      VYK+GD G VT +  S  +EEGD+R++  E+L E Y +L K DIF+LG 
Sbjct: 338 EEEDESHSSGVVYKIGDLGHVTSI-SSPQVEEGDSRFLAYEVLREDYTHLPKADIFALGL 397

Query: 463 AIYEIARGSTLPET--HFMNLKEGKLPLLPGHSLQ--FQNLIKAMVDPDPTRRPSAREVL 500
            +   A  S LP+    +  L++G+LP LP H L   F++L+K+++DPDPT RPSA  + 
Sbjct: 398 TVLLAAGASPLPQNGDDWHRLRQGELPNLP-HELPALFKDLLKSLLDPDPTARPSATALC 457

BLAST of CaUC09G163850 vs. ExPASy Swiss-Prot
Match: Q8QGV2 (Wee1-like protein kinase 1-B OS=Xenopus laevis OX=8355 GN=wee1-b PE=1 SV=1)

HSP 1 Score: 196.1 bits (497), Expect = 9.8e-49
Identity = 101/277 (36.46%), Postives = 158/277 (57.04%), Query Frame = 0

Query: 244 SRYHTDFHEIKQIGTGNFSRVFKVLKRIDGCLYAVKQSTRPLNQDTERRRALMEVQALAA 303
           SRY T+FHE+++IG+G F  VFK +KR+DGC+YA+K+S +PL    + + AL EV A A 
Sbjct: 242 SRYATEFHELEKIGSGEFGSVFKCVKRLDGCIYAIKRSKKPLAGSVDEQNALREVYAHAV 301

Query: 304 LGSHENIVGYYSSWFENEQLYIQMELC------DCSLSIGRYSHPFSEVDALRALYQIAK 363
           LG H ++V YYS+W E++ + IQ E C      D      R    F+E +    L Q+A+
Sbjct: 302 LGQHPHVVRYYSAWAEDDHMLIQNEYCNGGSLSDVISENYRTMQYFTEPELKDLLLQVAR 361

Query: 364 ALLFVHEKGVAHLDVKPDNIYVK--------------------DGVYKLGDFGCVTLLDK 423
            L ++H   + H+D+KP NI++                       +YK+GD G VT +  
Sbjct: 362 GLKYIHSMSLVHMDIKPSNIFISRTTLPNTAVEEADDEECGSGKVIYKIGDLGHVTRV-S 421

Query: 424 SLPIEEGDARYMPQEILNERYDYLDKVDIFSLGAAIYEIARGSTLPET--HFMNLKEGKL 483
           S  +EEGD+R++  E+L E Y +L K DIF+L   ++  A     P     +  +++GKL
Sbjct: 422 SPQVEEGDSRFLANEVLQENYTHLAKADIFALALTVWSAAGAEPFPTNGDQWHEIRQGKL 481

Query: 484 PLLPG-HSLQFQNLIKAMVDPDPTRRPSAREVLENPI 492
           P +P   S +F +LIK M+ PDP +RPS+  ++++ +
Sbjct: 482 PRVPQLLSQEFVDLIKLMISPDPEKRPSSVALVKHSV 517

BLAST of CaUC09G163850 vs. ExPASy TrEMBL
Match: A0A1S3BRY6 (wee1-like protein kinase OS=Cucumis melo OX=3656 GN=LOC103492846 PE=3 SV=1)

HSP 1 Score: 927.5 bits (2396), Expect = 2.3e-266
Identity = 462/501 (92.22%), Postives = 475/501 (94.81%), Query Frame = 0

Query: 1   MRRKSKTLRTTMTTRRKKMKGTLTRHLQVQLGQVSLAPLHLHRSSSAAAACFQDLPESEA 60
           MRRKSKTLRTTM TRRKKMKGT+TRHLQVQLG+VSLAPL LHRSSS +AACFQ+LPESEA
Sbjct: 1   MRRKSKTLRTTMGTRRKKMKGTVTRHLQVQLGKVSLAPLQLHRSSS-SAACFQNLPESEA 60

Query: 61  PPTVNVSDPLPSSSPNFEDRDFILSQDFFCTPDYITPDNQNLLNGVDNNEECIPCPKSPE 120
           P TVNVSD LPSSS NFEDRDFILSQDFFCTPDYITPDNQNLLNGVD+N+ECIPCPKSPE
Sbjct: 61  PSTVNVSDALPSSSSNFEDRDFILSQDFFCTPDYITPDNQNLLNGVDSNKECIPCPKSPE 120

Query: 121 KINTVKTKRKIHESVLVHPLSPSLSGCEQVVELGNDMFGSDDVDMEKETMAMPKKGNYVS 180
           KINTVKTKRKIHE+VLV PLSPSLSGCEQVVELGND FGSDDVDMEKETMAMPKK +YVS
Sbjct: 121 KINTVKTKRKIHENVLVRPLSPSLSGCEQVVELGNDTFGSDDVDMEKETMAMPKKESYVS 180

Query: 181 QSAVALRCRVMPPPCMKNPYLKDASDVGVDPFGNQRTKCAGTVQLVLKDIASFFPALFSG 240
           QSAVALRCRVMPPPCMKNPYL DASDVGVDPFGNQRTKCAG           FFPALFSG
Sbjct: 181 QSAVALRCRVMPPPCMKNPYLGDASDVGVDPFGNQRTKCAG-----------FFPALFSG 240

Query: 241 DGLSRYHTDFHEIKQIGTGNFSRVFKVLKRIDGCLYAVKQSTRPLNQDTERRRALMEVQA 300
           DGLSRYHTDFHEIK IGTGNFSRVFKVLKRIDGCLYAVKQSTRPLNQDTERRRALMEVQA
Sbjct: 241 DGLSRYHTDFHEIKLIGTGNFSRVFKVLKRIDGCLYAVKQSTRPLNQDTERRRALMEVQA 300

Query: 301 LAALGSHENIVGYYSSWFENEQLYIQMELCDCSLSIGRYSHPFSEVDALRALYQIAKALL 360
           LAALGSHENIVGYY+SWFENEQLYIQMELCDCSLS+G YSHPFSEVDALRALYQIAKALL
Sbjct: 301 LAALGSHENIVGYYTSWFENEQLYIQMELCDCSLSMGTYSHPFSEVDALRALYQIAKALL 360

Query: 361 FVHEKGVAHLDVKPDNIYVKDGVYKLGDFGCVTLLDKSLPIEEGDARYMPQEILNERYDY 420
           FVHEKG+AHLDVKPDNIY+KDGVYKLGDFGCVTLLDKSLPIEEGDARYMPQEILNERYDY
Sbjct: 361 FVHEKGIAHLDVKPDNIYIKDGVYKLGDFGCVTLLDKSLPIEEGDARYMPQEILNERYDY 420

Query: 421 LDKVDIFSLGAAIYEIARGSTLPETHFMNLKEGKLPLLPGHSLQFQNLIKAMVDPDPTRR 480
           LDKVDIFSLGAAIYEI RG TLPETHFMNLKEGKLPLLPGHSLQFQNLIKAMVDP+PTRR
Sbjct: 421 LDKVDIFSLGAAIYEIVRGFTLPETHFMNLKEGKLPLLPGHSLQFQNLIKAMVDPNPTRR 480

Query: 481 PSAREVLENPIFDKVRNHKQS 502
           PSAREVLENPIFDKVR HKQS
Sbjct: 481 PSAREVLENPIFDKVRTHKQS 489

BLAST of CaUC09G163850 vs. ExPASy TrEMBL
Match: A0A0A0K5B3 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G395770 PE=3 SV=1)

HSP 1 Score: 923.7 bits (2386), Expect = 3.3e-265
Identity = 457/501 (91.22%), Postives = 473/501 (94.41%), Query Frame = 0

Query: 1   MRRKSKTLRTTMTTRRKKMKGTLTRHLQVQLGQVSLAPLHLHRSSSAAAACFQDLPESEA 60
           MRRKSKTLRTTM  RRKKMKGT+TRHLQVQLG+VSLAPLHL+RSSS +A CF  LPESE 
Sbjct: 1   MRRKSKTLRTTMGARRKKMKGTVTRHLQVQLGKVSLAPLHLNRSSS-SALCFPSLPESEP 60

Query: 61  PPTVNVSDPLPSSSPNFEDRDFILSQDFFCTPDYITPDNQNLLNGVDNNEECIPCPKSPE 120
           P TVN SDPLPSSS NFEDRDFILSQDFFCTPDYITPDNQNLLNGVD+N+ECIPCPKSPE
Sbjct: 61  PSTVNFSDPLPSSSHNFEDRDFILSQDFFCTPDYITPDNQNLLNGVDSNKECIPCPKSPE 120

Query: 121 KINTVKTKRKIHESVLVHPLSPSLSGCEQVVELGNDMFGSDDVDMEKETMAMPKKGNYVS 180
           K+NTVKTKRKIHE+VLV PLSPSLSGCEQVVELGND+FGSDDVDMEKET AMPKK NYVS
Sbjct: 121 KVNTVKTKRKIHENVLVRPLSPSLSGCEQVVELGNDIFGSDDVDMEKETTAMPKKENYVS 180

Query: 181 QSAVALRCRVMPPPCMKNPYLKDASDVGVDPFGNQRTKCAGTVQLVLKDIASFFPALFSG 240
           QSAVALRCRVMPPPCMKNPYLKDASDVGVDPFGNQRTKCAG           FFPALFSG
Sbjct: 181 QSAVALRCRVMPPPCMKNPYLKDASDVGVDPFGNQRTKCAG-----------FFPALFSG 240

Query: 241 DGLSRYHTDFHEIKQIGTGNFSRVFKVLKRIDGCLYAVKQSTRPLNQDTERRRALMEVQA 300
           DGLSRYHTDFHEIK IGTGNFSRVFKVLKRIDGCLYAVKQSTRPLNQDTERRRALMEVQA
Sbjct: 241 DGLSRYHTDFHEIKLIGTGNFSRVFKVLKRIDGCLYAVKQSTRPLNQDTERRRALMEVQA 300

Query: 301 LAALGSHENIVGYYSSWFENEQLYIQMELCDCSLSIGRYSHPFSEVDALRALYQIAKALL 360
           LAALG+HENIVGYY+SWFENEQLYIQMELCDCSLS+GRYSHPFSEVDALRALYQIAKALL
Sbjct: 301 LAALGAHENIVGYYTSWFENEQLYIQMELCDCSLSMGRYSHPFSEVDALRALYQIAKALL 360

Query: 361 FVHEKGVAHLDVKPDNIYVKDGVYKLGDFGCVTLLDKSLPIEEGDARYMPQEILNERYDY 420
           FVHEKG+AHLDVKPDNIY+KDGVYKLGDFGCVTLLDKSLPIEEGDARYMPQEILNERYDY
Sbjct: 361 FVHEKGIAHLDVKPDNIYIKDGVYKLGDFGCVTLLDKSLPIEEGDARYMPQEILNERYDY 420

Query: 421 LDKVDIFSLGAAIYEIARGSTLPETHFMNLKEGKLPLLPGHSLQFQNLIKAMVDPDPTRR 480
           LDKVDIFSLGAAIYEI RG TLPETHFMNLKEGKLPLLPGHSLQFQNLIKAMVDPDP+RR
Sbjct: 421 LDKVDIFSLGAAIYEIVRGFTLPETHFMNLKEGKLPLLPGHSLQFQNLIKAMVDPDPSRR 480

Query: 481 PSAREVLENPIFDKVRNHKQS 502
           PSAREV+ENPIFDKVR HKQS
Sbjct: 481 PSAREVIENPIFDKVRTHKQS 489

BLAST of CaUC09G163850 vs. ExPASy TrEMBL
Match: A0A5A7VN66 (Wee1-like protein kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold138G002220 PE=3 SV=1)

HSP 1 Score: 910.6 bits (2352), Expect = 2.9e-261
Identity = 451/490 (92.04%), Postives = 464/490 (94.69%), Query Frame = 0

Query: 12  MTTRRKKMKGTLTRHLQVQLGQVSLAPLHLHRSSSAAAACFQDLPESEAPPTVNVSDPLP 71
           M TRRKKMKGT+TRHLQVQLG+VSLAPL LHRSSS +AACFQ+LPESEAP TVNVSD LP
Sbjct: 1   MGTRRKKMKGTVTRHLQVQLGKVSLAPLQLHRSSS-SAACFQNLPESEAPSTVNVSDALP 60

Query: 72  SSSPNFEDRDFILSQDFFCTPDYITPDNQNLLNGVDNNEECIPCPKSPEKINTVKTKRKI 131
           SSS NFEDRDFILSQDFFCTPDYITPDNQNLLNGVD+N+ECIPCPKSPEKINTVKTKRKI
Sbjct: 61  SSSSNFEDRDFILSQDFFCTPDYITPDNQNLLNGVDSNKECIPCPKSPEKINTVKTKRKI 120

Query: 132 HESVLVHPLSPSLSGCEQVVELGNDMFGSDDVDMEKETMAMPKKGNYVSQSAVALRCRVM 191
           HE+VLV PLSPSLSGCEQVVELGND FGSDDVDMEKETMAMPKK +YVSQSAVALRCRVM
Sbjct: 121 HENVLVRPLSPSLSGCEQVVELGNDTFGSDDVDMEKETMAMPKKESYVSQSAVALRCRVM 180

Query: 192 PPPCMKNPYLKDASDVGVDPFGNQRTKCAGTVQLVLKDIASFFPALFSGDGLSRYHTDFH 251
           PPPCMKNPYL DASDVGVDPFGNQRTKCAG           FFPALFSGDGLSRYHTDFH
Sbjct: 181 PPPCMKNPYLGDASDVGVDPFGNQRTKCAG-----------FFPALFSGDGLSRYHTDFH 240

Query: 252 EIKQIGTGNFSRVFKVLKRIDGCLYAVKQSTRPLNQDTERRRALMEVQALAALGSHENIV 311
           EIK IGTGNFSRVFKVLKRIDGCLYAVKQSTRPLNQDTERRRALMEVQALAALGSHENIV
Sbjct: 241 EIKLIGTGNFSRVFKVLKRIDGCLYAVKQSTRPLNQDTERRRALMEVQALAALGSHENIV 300

Query: 312 GYYSSWFENEQLYIQMELCDCSLSIGRYSHPFSEVDALRALYQIAKALLFVHEKGVAHLD 371
           GYY+SWFENEQLYIQMELCDCSLS+G YSHPFSEVDALRALYQIAKALLFVHEKG+AHLD
Sbjct: 301 GYYTSWFENEQLYIQMELCDCSLSMGTYSHPFSEVDALRALYQIAKALLFVHEKGIAHLD 360

Query: 372 VKPDNIYVKDGVYKLGDFGCVTLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLGA 431
           VKPDNIY+KDGVYKLGDFGCVTLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLGA
Sbjct: 361 VKPDNIYIKDGVYKLGDFGCVTLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLGA 420

Query: 432 AIYEIARGSTLPETHFMNLKEGKLPLLPGHSLQFQNLIKAMVDPDPTRRPSAREVLENPI 491
           AIYEI RG TLPETHFMNLKEGKLPLLPGHSLQFQNLIKAMVDP+PTRRPSAREVLENPI
Sbjct: 421 AIYEIVRGFTLPETHFMNLKEGKLPLLPGHSLQFQNLIKAMVDPNPTRRPSAREVLENPI 478

Query: 492 FDKVRNHKQS 502
           FDKVR HKQS
Sbjct: 481 FDKVRTHKQS 478

BLAST of CaUC09G163850 vs. ExPASy TrEMBL
Match: A0A5D3D2H5 (Wee1-like protein kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G00240 PE=4 SV=1)

HSP 1 Score: 885.6 bits (2287), Expect = 9.9e-254
Identity = 438/478 (91.63%), Postives = 450/478 (94.14%), Query Frame = 0

Query: 24  TRHLQVQLGQVSLAPLHLHRSSSAAAACFQDLPESEAPPTVNVSDPLPSSSPNFEDRDFI 83
           T H  VQLG+VSLAPL LHRSSS +AACFQ+LPESEAP TVNVSD LPSSS NFEDRDFI
Sbjct: 166 TFHNVVQLGKVSLAPLQLHRSSS-SAACFQNLPESEAPSTVNVSDALPSSSSNFEDRDFI 225

Query: 84  LSQDFFCTPDYITPDNQNLLNGVDNNEECIPCPKSPEKINTVKTKRKIHESVLVHPLSPS 143
           LSQDFFCTPDYITPDNQNLLNGVD+N+ECIPCPKSPEKINTVKTKRKIHE+VLV PLSPS
Sbjct: 226 LSQDFFCTPDYITPDNQNLLNGVDSNKECIPCPKSPEKINTVKTKRKIHENVLVRPLSPS 285

Query: 144 LSGCEQVVELGNDMFGSDDVDMEKETMAMPKKGNYVSQSAVALRCRVMPPPCMKNPYLKD 203
           LSGCEQVVELGND FGSDDVDMEKETMAMPKK +YVSQSAVALRCRVMPPPCMKNPYL D
Sbjct: 286 LSGCEQVVELGNDTFGSDDVDMEKETMAMPKKESYVSQSAVALRCRVMPPPCMKNPYLGD 345

Query: 204 ASDVGVDPFGNQRTKCAGTVQLVLKDIASFFPALFSGDGLSRYHTDFHEIKQIGTGNFSR 263
           ASDVGVDPFGNQRTKCAG           FFPALFSGDGLSRYHTDFHEIK IGTGNFSR
Sbjct: 346 ASDVGVDPFGNQRTKCAG-----------FFPALFSGDGLSRYHTDFHEIKLIGTGNFSR 405

Query: 264 VFKVLKRIDGCLYAVKQSTRPLNQDTERRRALMEVQALAALGSHENIVGYYSSWFENEQL 323
           VFKVLKRIDGCLYAVKQSTRPLNQDTERRRALMEVQALAALGSHENIVGYY+SWFENEQL
Sbjct: 406 VFKVLKRIDGCLYAVKQSTRPLNQDTERRRALMEVQALAALGSHENIVGYYTSWFENEQL 465

Query: 324 YIQMELCDCSLSIGRYSHPFSEVDALRALYQIAKALLFVHEKGVAHLDVKPDNIYVKDGV 383
           YIQMELCDCSLS+G YSHPFSEVDALRALYQIAKALLFVHEKG+AHLDVKPDNIY+KDGV
Sbjct: 466 YIQMELCDCSLSMGTYSHPFSEVDALRALYQIAKALLFVHEKGIAHLDVKPDNIYIKDGV 525

Query: 384 YKLGDFGCVTLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLGAAIYEIARGSTLP 443
           YKLGDFGCVTLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLGAAIYEI RG TLP
Sbjct: 526 YKLGDFGCVTLLDKSLPIEEGDARYMPQEILNERYDYLDKVDIFSLGAAIYEIVRGFTLP 585

Query: 444 ETHFMNLKEGKLPLLPGHSLQFQNLIKAMVDPDPTRRPSAREVLENPIFDKVRNHKQS 502
           ETHFMNLKEGKLPLLPGHSLQFQNLIKAMVDP+PTRRPSAREVLENPIFDKVR HKQS
Sbjct: 586 ETHFMNLKEGKLPLLPGHSLQFQNLIKAMVDPNPTRRPSAREVLENPIFDKVRTHKQS 631

BLAST of CaUC09G163850 vs. ExPASy TrEMBL
Match: A0A6J1H582 (wee1-like protein kinase OS=Cucurbita moschata OX=3662 GN=LOC111460604 PE=3 SV=1)

HSP 1 Score: 885.6 bits (2287), Expect = 9.9e-254
Identity = 443/501 (88.42%), Postives = 456/501 (91.02%), Query Frame = 0

Query: 1   MRRKSKTLRTTMTTRRKKMKGTLTRHLQVQLGQVSLAPLHLHRSSSAAAACFQDLPESEA 60
           MRRKSKTLRTTMT  RKKMKGTLTRHLQVQLGQVSLAPLH H+ SS+ AACFQDLPESEA
Sbjct: 1   MRRKSKTLRTTMTATRKKMKGTLTRHLQVQLGQVSLAPLH-HQRSSSTAACFQDLPESEA 60

Query: 61  PPTVNVSDPLPSSSPNFEDRDFILSQDFFCTPDYITPDNQNLLNGVDNNEECIPCPKSPE 120
             TVN S  LPSSS NFEDRDFILSQDFFCTPDYITPDNQNLLNGVD  +E  PCPKSPE
Sbjct: 61  MATVNDSGALPSSSTNFEDRDFILSQDFFCTPDYITPDNQNLLNGVDCYKESTPCPKSPE 120

Query: 121 KINTVKTKRKIHESVLVHPLSPSLSGCEQVVELGNDMFGSDDVDMEKETMAMPKKGNYVS 180
           KINTVKTKRKI + VLVHPLSPSLSG EQVVELGND FG+DD DMEKETMAMPKK NYVS
Sbjct: 121 KINTVKTKRKIRDGVLVHPLSPSLSGREQVVELGNDTFGTDDEDMEKETMAMPKKQNYVS 180

Query: 181 QSAVALRCRVMPPPCMKNPYLKDASDVGVDPFGNQRTKCAGTVQLVLKDIASFFPALFSG 240
           QSAVALRCRVMPPPCMKNPYL DASD+GVDPFGNQRTKC           A FFPALFSG
Sbjct: 181 QSAVALRCRVMPPPCMKNPYLNDASDIGVDPFGNQRTKC-----------ADFFPALFSG 240

Query: 241 DGLSRYHTDFHEIKQIGTGNFSRVFKVLKRIDGCLYAVKQSTRPLNQDTERRRALMEVQA 300
           DGLSRYHTDFHEIKQIGTGNFSRVFKVLKRIDGCLYAVKQSTRPL+QD ERRRALMEVQA
Sbjct: 241 DGLSRYHTDFHEIKQIGTGNFSRVFKVLKRIDGCLYAVKQSTRPLHQDIERRRALMEVQA 300

Query: 301 LAALGSHENIVGYYSSWFENEQLYIQMELCDCSLSIGRYSHPFSEVDALRALYQIAKALL 360
           LAALGSHENIVGYYSSWFENEQLYIQMELCDCSLS GRYSHPFSE DAL+AL+QIAKALL
Sbjct: 301 LAALGSHENIVGYYSSWFENEQLYIQMELCDCSLSTGRYSHPFSEADALKALHQIAKALL 360

Query: 361 FVHEKGVAHLDVKPDNIYVKDGVYKLGDFGCVTLLDKSLPIEEGDARYMPQEILNERYDY 420
           F+HEKGV HLDVKPDNIYVKDGVYKLGDFGC TLLDKSLPIEEGDARYMPQEILNERYDY
Sbjct: 361 FMHEKGVVHLDVKPDNIYVKDGVYKLGDFGCATLLDKSLPIEEGDARYMPQEILNERYDY 420

Query: 421 LDKVDIFSLGAAIYEIARGSTLPETHFMNLKEGKLPLLPGHSLQFQNLIKAMVDPDPTRR 480
           LDKVDIFSLGAAIYE+ RGST PETHFMNLKEGKLPL P HSLQFQNLIKAMVDPDP RR
Sbjct: 421 LDKVDIFSLGAAIYEVVRGSTFPETHFMNLKEGKLPLFPCHSLQFQNLIKAMVDPDPARR 480

Query: 481 PSAREVLENPIFDKVRNHKQS 502
           PSAREVL+NPIFDKVRNH +S
Sbjct: 481 PSAREVLQNPIFDKVRNHPKS 489

BLAST of CaUC09G163850 vs. TAIR 10
Match: AT1G02970.1 (WEE1 kinase homolog )

HSP 1 Score: 569.3 bits (1466), Expect = 3.0e-162
Identity = 295/499 (59.12%), Postives = 369/499 (73.95%), Query Frame = 0

Query: 2   RRKSKTLRTTMTTRRKKMKGTLTRHLQVQLGQVSLAPLHLHRSSSAAAACFQDLPESEAP 61
           R+   T++T  + + +KM+GTL RH  +Q GQ+S        SS+ A++ FQ L +S++ 
Sbjct: 13  RKTQGTIKTRASKKIRKMEGTLERHSLLQFGQLSKISFENRPSSNVASSAFQGLLDSDSS 72

Query: 62  PTVNVSDPLPSSSPNFEDRDFILSQDFFCTPDYITPDNQNLLNGVDNNEECIPCPKSPEK 121
              N      S + N  ++DFILSQDFFCTPDYITPDNQNL++G+D +++  PCP+SP K
Sbjct: 73  ELRNQLGSADSDA-NCGEKDFILSQDFFCTPDYITPDNQNLMSGLDISKDHSPCPRSPVK 132

Query: 122 INTVKTKRKIHESVLVHPLSPSLSGCEQVVELGNDMFGSDDVDMEKETMAMPKKGNYVSQ 181
           +NTVK+KR   ES   +  + + S   +V E  ND   +D+V  +K      ++  YVSQ
Sbjct: 133 LNTVKSKRCRQESFTGNHSNSTWSSKHRVDEQENDDIDTDEVMGDKLQANQTERTGYVSQ 192

Query: 182 SAVALRCRVMPPPCMKNPYLKDASDVGVDPFGNQRTKCAGTVQLVLKDIASFFPALFSGD 241
           +AVALRCR MPPPC+KNPY+ + S+   DPFG+QR+KC           ASF P   SGD
Sbjct: 193 AAVALRCRAMPPPCLKNPYVLNQSETATDPFGHQRSKC-----------ASFLPVSTSGD 252

Query: 242 GLSRYHTDFHEIKQIGTGNFSRVFKVLKRIDGCLYAVKQSTRPLNQDTERRRALMEVQAL 301
           GLSRY TDFHEI+QIG G+FSRVFKVLKR+DGCLYAVK STR L  D+ERR+A+MEVQAL
Sbjct: 253 GLSRYLTDFHEIRQIGAGHFSRVFKVLKRMDGCLYAVKHSTRKLYLDSERRKAMMEVQAL 312

Query: 302 AALGSHENIVGYYSSWFENEQLYIQMELCDCSLSI--GRYSHPFSEVDALRALYQIAKAL 361
           AALG HENIVGYYSSWFENEQLYIQ+ELCD SLS    + S   SE + L  ++QIAKAL
Sbjct: 313 AALGFHENIVGYYSSWFENEQLYIQLELCDHSLSALPKKSSLKVSEREILVIMHQIAKAL 372

Query: 362 LFVHEKGVAHLDVKPDNIYVKDGVYKLGDFGCVTLLDKSLPIEEGDARYMPQEILNERYD 421
            FVHEKG+AHLDVKPDNIY+K+GV KLGDFGC T LDKSLP+EEGDARYMPQEILNE Y+
Sbjct: 373 HFVHEKGIAHLDVKPDNIYIKNGVCKLGDFGCATRLDKSLPVEEGDARYMPQEILNEDYE 432

Query: 422 YLDKVDIFSLGAAIYEIARGSTLPET--HFMNLKEGKLPLLPGHSLQFQNLIKAMVDPDP 481
           +LDKVDIFSLG  +YE+ +GS L E+    +N+KEGKLPLLPGHSLQ Q L+K M+D DP
Sbjct: 433 HLDKVDIFSLGVTVYELIKGSPLTESRNQSLNIKEGKLPLLPGHSLQLQQLLKTMMDRDP 492

Query: 482 TRRPSAREVLENPIFDKVR 497
            RRPSARE+L++P+FD++R
Sbjct: 493 KRRPSARELLDHPMFDRIR 499

BLAST of CaUC09G163850 vs. TAIR 10
Match: AT4G19110.1 (Protein kinase superfamily protein )

HSP 1 Score: 107.5 bits (267), Expect = 3.3e-23
Identity = 80/279 (28.67%), Postives = 132/279 (47.31%), Query Frame = 0

Query: 253 IKQIGTGNFSRVFKVLKRIDGCLYAVKQSTRPLNQDTERRRALMEVQALAALGSHENIVG 312
           IK++G G F  V++ + +  G + A+K+  +      E    L EV++L  + +H NIV 
Sbjct: 7   IKEVGDGTFGSVWRAINKQTGEVVAIKKMKKKYYSWDECIN-LREVKSLRRM-NHPNIVK 66

Query: 313 YYSSWFENEQLYIQMELCDCSL--SIGRYSHPFSEVDALRALYQIAKALLFVHEKGVAHL 372
                 EN+ LY   E  +C+L   +      F+E D     +Q+ + L ++H++G  H 
Sbjct: 67  LKEVIRENDILYFVFEYMECNLYQLMKDRQKLFAEADIKNWCFQVFQGLSYMHQRGYFHR 126

Query: 373 DVKPDNIYVKDGVYKLGDFGCVTLLDKSLPIEE--GDARYMPQEILNERYDYLDKVDIFS 432
           D+KP+N+ V   + K+ DFG    ++ S P  E      Y   E+L + Y Y  KVD+++
Sbjct: 127 DLKPENLLVSKDIIKIADFGLAREVNSSPPFTEYVSTRWYRAPEVLLQSYVYTSKVDMWA 186

Query: 433 LGA-------------------AIYEIAR--GSTLPET--------HFMNLKEGKLP--- 492
           +GA                    IY+I    G+   ET        + +N +  +LP   
Sbjct: 187 MGAIMAELLSLRPIFPGASEADEIYKICSVIGTPTEETWLEGLNLANTINYQFPQLPGVP 246

BLAST of CaUC09G163850 vs. TAIR 10
Match: AT4G19110.2 (Protein kinase superfamily protein )

HSP 1 Score: 107.5 bits (267), Expect = 3.3e-23
Identity = 80/279 (28.67%), Postives = 132/279 (47.31%), Query Frame = 0

Query: 253 IKQIGTGNFSRVFKVLKRIDGCLYAVKQSTRPLNQDTERRRALMEVQALAALGSHENIVG 312
           IK++G G F  V++ + +  G + A+K+  +      E    L EV++L  + +H NIV 
Sbjct: 7   IKEVGDGTFGSVWRAINKQTGEVVAIKKMKKKYYSWDECIN-LREVKSLRRM-NHPNIVK 66

Query: 313 YYSSWFENEQLYIQMELCDCSL--SIGRYSHPFSEVDALRALYQIAKALLFVHEKGVAHL 372
                 EN+ LY   E  +C+L   +      F+E D     +Q+ + L ++H++G  H 
Sbjct: 67  LKEVIRENDILYFVFEYMECNLYQLMKDRQKLFAEADIKNWCFQVFQGLSYMHQRGYFHR 126

Query: 373 DVKPDNIYVKDGVYKLGDFGCVTLLDKSLPIEE--GDARYMPQEILNERYDYLDKVDIFS 432
           D+KP+N+ V   + K+ DFG    ++ S P  E      Y   E+L + Y Y  KVD+++
Sbjct: 127 DLKPENLLVSKDIIKIADFGLAREVNSSPPFTEYVSTRWYRAPEVLLQSYVYTSKVDMWA 186

Query: 433 LGA-------------------AIYEIAR--GSTLPET--------HFMNLKEGKLP--- 492
           +GA                    IY+I    G+   ET        + +N +  +LP   
Sbjct: 187 MGAIMAELLSLRPIFPGASEADEIYKICSVIGTPTEETWLEGLNLANTINYQFPQLPGVP 246

BLAST of CaUC09G163850 vs. TAIR 10
Match: AT5G45430.1 (Protein kinase superfamily protein )

HSP 1 Score: 101.7 bits (252), Expect = 1.8e-21
Identity = 78/279 (27.96%), Postives = 123/279 (44.09%), Query Frame = 0

Query: 253 IKQIGTGNFSRVFKVLKRIDGCLYAVKQSTRPLNQDTERRRALMEVQALAALGSHENIVG 312
           +K++G G F  V++ + +    + A+K+  +      E    L EV++L+ + +H NIV 
Sbjct: 7   LKEVGDGTFGNVWRAVNKQTNEVVAIKRMKKKY-FSWEECVNLREVKSLSRM-NHPNIVK 66

Query: 313 YYSSWFENEQLYIQMELCDCSLSIGRYSHP--FSEVDALRALYQIAKALLFVHEKGVAHL 372
                 EN+ LY   E  +C+L       P  F+E D     +Q+ + L ++H++G  H 
Sbjct: 67  LKEVIRENDILYFVFEYMECNLYQLMKDRPKHFAESDIRNWCFQVFQGLSYMHQRGYFHR 126

Query: 373 DVKPDNIYVKDGVYKLGDFGCVTLLDKSLPIEE--GDARYMPQEILNERYDYLDKVDIFS 432
           D+KP+N+ V   V K+ D G    +D S P  E      Y   E+L + Y Y  KVD+++
Sbjct: 127 DLKPENLLVSKDVIKIADLGLAREIDSSPPYTEYVSTRWYRAPEVLLQSYVYTSKVDMWA 186

Query: 433 LGA-------------------AIYEIAR--GSTLPETHFMNLK-----EGKLPLLPG-- 492
           +GA                    IY+I    GS   ET    L        + P  PG  
Sbjct: 187 MGAIMAELLSLRPLFPGASEADEIYKICSVIGSPTEETWLEGLNLASVINYQFPQFPGVH 246

BLAST of CaUC09G163850 vs. TAIR 10
Match: AT5G45430.2 (Protein kinase superfamily protein )

HSP 1 Score: 101.7 bits (252), Expect = 1.8e-21
Identity = 78/279 (27.96%), Postives = 123/279 (44.09%), Query Frame = 0

Query: 253 IKQIGTGNFSRVFKVLKRIDGCLYAVKQSTRPLNQDTERRRALMEVQALAALGSHENIVG 312
           +K++G G F  V++ + +    + A+K+  +      E    L EV++L+ + +H NIV 
Sbjct: 7   LKEVGDGTFGNVWRAVNKQTNEVVAIKRMKKKY-FSWEECVNLREVKSLSRM-NHPNIVK 66

Query: 313 YYSSWFENEQLYIQMELCDCSLSIGRYSHP--FSEVDALRALYQIAKALLFVHEKGVAHL 372
                 EN+ LY   E  +C+L       P  F+E D     +Q+ + L ++H++G  H 
Sbjct: 67  LKEVIRENDILYFVFEYMECNLYQLMKDRPKHFAESDIRNWCFQVFQGLSYMHQRGYFHR 126

Query: 373 DVKPDNIYVKDGVYKLGDFGCVTLLDKSLPIEE--GDARYMPQEILNERYDYLDKVDIFS 432
           D+KP+N+ V   V K+ D G    +D S P  E      Y   E+L + Y Y  KVD+++
Sbjct: 127 DLKPENLLVSKDVIKIADLGLAREIDSSPPYTEYVSTRWYRAPEVLLQSYVYTSKVDMWA 186

Query: 433 LGA-------------------AIYEIAR--GSTLPETHFMNLK-----EGKLPLLPG-- 492
           +GA                    IY+I    GS   ET    L        + P  PG  
Sbjct: 187 MGAIMAELLSLRPLFPGASEADEIYKICSVIGSPTEETWLEGLNLASVINYQFPQFPGVH 246

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038876752.12.3e-27394.23wee1-like protein kinase [Benincasa hispida][more]
XP_008451611.14.7e-26692.22PREDICTED: wee1-like protein kinase [Cucumis melo][more]
XP_004136046.16.8e-26591.22wee1-like protein kinase isoform X2 [Cucumis sativus] >KGN44910.1 hypothetical p... [more]
XP_031744240.11.7e-26391.04wee1-like protein kinase isoform X1 [Cucumis sativus][more]
KAA0068026.15.9e-26192.04wee1-like protein kinase [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
Q8L4H04.3e-16159.12Wee1-like protein kinase OS=Arabidopsis thaliana OX=3702 GN=WEE1 PE=1 SV=1[more]
Q6Z8292.5e-13754.67Wee1-like protein kinase OS=Oryza sativa subsp. japonica OX=39947 GN=WEE1 PE=3 S... [more]
P543504.0e-5036.90Wee1-like protein kinase OS=Drosophila melanogaster OX=7227 GN=Wee1 PE=1 SV=2[more]
Q1LX512.0e-4937.18Wee1-like protein kinase 2 OS=Danio rerio OX=7955 GN=wee2 PE=2 SV=2[more]
Q8QGV29.8e-4936.46Wee1-like protein kinase 1-B OS=Xenopus laevis OX=8355 GN=wee1-b PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3BRY62.3e-26692.22wee1-like protein kinase OS=Cucumis melo OX=3656 GN=LOC103492846 PE=3 SV=1[more]
A0A0A0K5B33.3e-26591.22Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G395... [more]
A0A5A7VN662.9e-26192.04Wee1-like protein kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffol... [more]
A0A5D3D2H59.9e-25491.63Wee1-like protein kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol... [more]
A0A6J1H5829.9e-25488.42wee1-like protein kinase OS=Cucurbita moschata OX=3662 GN=LOC111460604 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G02970.13.0e-16259.12WEE1 kinase homolog [more]
AT4G19110.13.3e-2328.67Protein kinase superfamily protein [more]
AT4G19110.23.3e-2328.67Protein kinase superfamily protein [more]
AT5G45430.11.8e-2127.96Protein kinase superfamily protein [more]
AT5G45430.21.8e-2127.96Protein kinase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (USVL246-FR2) v1
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 250..492
e-value: 1.6E-44
score: 163.9
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 250..492
e-value: 3.5E-47
score: 161.0
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 250..492
score: 36.286938
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 330..497
e-value: 3.1E-36
score: 126.6
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 240..329
e-value: 6.3E-32
score: 111.5
NoneNo IPR availablePANTHERPTHR11042:SF155BNAA08G27310D PROTEINcoord: 19..408
coord: 421..497
NoneNo IPR availablePANTHERPTHR11042EUKARYOTIC TRANSLATION INITIATION FACTOR 2-ALPHA KINASE EIF2-ALPHA KINASE -RELATEDcoord: 19..408
coord: 421..497
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 256..279
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 367..379
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 243..496

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CaUC09G163850.1CaUC09G163850.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1902750 negative regulation of cell cycle G2/M phase transition
biological_process GO:0018108 peptidyl-tyrosine phosphorylation
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0005634 nucleus
molecular_function GO:0005524 ATP binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004713 protein tyrosine kinase activity
molecular_function GO:0004672 protein kinase activity