CaUC09G163230 (gene) Watermelon (USVL246-FR2) v1

Overview
NameCaUC09G163230
Typegene
OrganismCitrullus amarus (Watermelon (USVL246-FR2) v1)
DescriptionPectinesterase
LocationCiama_Chr09: 5039597 .. 5043901 (-)
RNA-Seq ExpressionCaUC09G163230
SyntenyCaUC09G163230
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTAAACTTCCCTCCTCTCTCCAATGGCTGCCAGAACAACCACCGCCGCCGCCTTCTACTTTCTCATCCTCATTTCCCTTTACATTTCCACTTCCGCCGCCCCCACCGGTTCAGTATGCAGCTCGACTCGAGACCCATCCTACTGCAAATCGGTTCTCCCGAATCAAACCGGCAATGTCTACTCGTACGGACGGTCGTCGTTCAGAAAGTCACTGTCGTCGTCGCAGAAATTCCTCCGGCTGGTGGAGAAATACCTCAGGTCCCGATCGTCGCTGACAGTGCCGGCGGTGAGGGCGCTGGAGGACTGCCAGCTGCTGGCGGGACTGAACATTGATTACTTAACGACGTCGTTCCGGGCGGTGAACTCGACGAGCAGAACGTTGCCGGAGATGAAGGCGGACGACGTGCAGAGTCTGCTGAGTGCGATATTGACGAATCAACAGACTTGTTTGGATGGAATTAAGGCGACGGCGGGTTCTTGGTCGCTCAAAAATGGCCTTTCTCTCCCACTTGCTGCTGACACCAAGCTCTACTCTCTGTCCTTGGCCTTCTTTACCAAAGGTTTCTCATTATTTTTCTTTTATTTTTAAAGGATAAGAAGAAAATTCTTTAACCCATCAAAAACAAAAAAAAAAAAAAACAAAGGATAGAGAGAAAAAAAACTAGCTTAGTTCTATTATATATTTTATTAATAATTTGATTTTAGAAAATAATTATACCTCTATACCTTAAATTAGTTTCTAAGAGTCTTTCATTATTATTATTATAGAAATTGATTAAATTAGTATAGTAGTTTTCAAAAAAAATAATATATATCTCCAATTTATAAAGGTTTATTTAAACTATCTAAACTTAAATTAGATCTGAAAATATAGGAAGCAAAATAAATATTAAAGTGGTATGTTAACTAAAATTAAAATGAAATGAGAGAGCAACTCTAGAGGCATTTGTCTAATTTATCTTTTAATTTTTTTTTCTTTTTTCTATAGACCATCAATTAATAGAGACTCTTAATTATTTATTTTAATTATTTTGGGATTTAATGAATTAGATAGGTATTTTAACTTTTGATCTTTTAGTCAAAATACTTGCTAGCTACCTTATTGATACTTTATTAAATGTAATAGTTAATTAATTTTTTAAAAGAAGAAAAATCTTAGGCAATAGTTTATAAGAGATTTGATTGATTTTTTTTTTTTTTTTTTTTTTTTTTGTTTATACACTAATATATTAATTAATTAATGCTACGATGAAAAGTTTTGTAATAACTTAAACATCCCTAAGAAATTTTGTAGAATTGTCCTTTTTTTTTTTTAAACATCGAAATGCCCTTCTTTTCTTTTGTTGAATAACAAACTTCTGTTGTAGTTAGGTGAATTTGTTGAAAGTTTTATTAGGCTTTCTTTTAATTTTTTAGGTATTAATTGTTGAAATTTGTCACCCATCCATGTGTCTCTTTTCTCAAGGCTTAACTTAAAAAAAAGTTATAAGTTGAAAATATCTTCCTTTTTCTAATATATATATATATATATATTGCATGAATTAAAATATTAAACACCTAGCAAATATAAAATATTTTCATTTTCAGTTAGAAAATAAGTAAACAAAAACTAATTCGTTTTTTTTTTTTCTTAATATCTTCGACAGGTTGGGTTCCAAAGAAGAAGAAAAAACCGACATGGAAAGCCGCCGGAAGACAAGGGGGGTTCAGAAATGGGCGAATGTCACTCAAAATGTCCAGCCGAACCAAAGCGATCTACGAGACGGCGACCCGCCGGAATCTGTTGCAGACCGATGACGGCGGCGACGACCAGATTGCGGTCAGGGACATCGTCGTTGTCAGTCAAGACGGCAGCGGAAATTTCACCACCATCAATGAGGCGATTTCGGCGGCGCCGAACAACTCTGCTCCGTCCGACGGGTATTTCTTGATCTTCGTGTCGGCCGGAATTTATGAAGAATATGTGTCGATTGCGAAGAACAAGAGGTACTTGATGATGATCGGAGATGGCATTAATCAGACCGTCGTCACCGGTAATCGGAGTGTCGTTGACGGCTGGACCACCTTCAATTCCGCCACGTTTGGTAAGTGTTTCCACCTTTAAATTTTATTAAATAACAATGTTAGGTTAGACCCGAATTTTTAAAAAAGTTTTCCATAGATTCCCTGATTTTTTATTTTTATTTTTTGGTATTTTTTGAACCTATAAGTTTGATTTTTTTTTTCTTTTTTTGTCTTTTACCTATTTCATAATTTTCTAAATCATCAAAATTTAGTTTAATATGTTATTAAATAAAATATTCAATTTTATGTTAAATAAATTTATTAATTTTAAATAATATTAAATTTGTTAATTATTTATTAAACACAACGTTAAAAGTTTCTAGAAATCATTAAATATAAAATTTAAAAATCCACGAACATATTAAATAGTTAATAGGCCAAATCTATAATTTATCTTACACTCAGAAATTGACTAAAGAAATGCTTAGATTTTTAATCATTACTTGATTTATAGTTATTATCGGTTTTTGGAACTTAGATTTTTAAACACTACTCCCATCAATTACATATAAATTTATTATTATTATTTTTTTTTATTAATCTTCTACTAAAGTTTAAAAAAAAAAAAAAAAAAAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAATCTTCTACTAAAGTTAAAAAAAAAAAAAAAAAAAAAGGTTGGCTTCATTGGAAAACAATTTGTTTTTTAATAATAAATTTGTTGTTTTTGAAAATTAAGTTGAAAACAATAATTTCCATCGATATGTTTTAAAAAGACTGTAAATTCTGAAAACTAAAAACAGTTCAAAAAACTTTTTTCCTTTTTTGCACTTGACTAGAAATTATGTATTAAGTATTTTTTTTTTTAAATTAACAAAGGTAAAAATTAAAAATAGAGGAAACAAACATAAAATTCAAAAAAAAAAATTAAAAACGAAATCGATATTATTAAAAAATAAAAACAAAAAATCTAATTTATCCCCTGTTTTCGTTTCATCACCAGCGGTGGTCGGACCTGGATTCGTAGCAGTAAACATGACATTCAGAAACACCGCCGGCGCCATAAAGCATCAAGCCGTAGCCGTCCGGAATGGCGCCGACTTATCAACATTCTACCTCTGCAGCTTCGAAGCCTACCAAGACACCTTATACACCCATTCCCTCCGCCAATTCTACCGCGACTGCGACATCTACGGCACCGTCGACTTCATCTTCGGCAACGCCGCCGTCGTCTTCCAGAACTGCAACATTTACCCACGCCTCCCGATGTCCAACCAGTTCAACGCCATCACCGCCCAGGGCCGGACCGACCCAAATCAGAACACCGGCACCTCCATCTACAACTGCCGAATCACGGCAGCCGACGATCTGGCCAACAACAGCGGCGCCGGCGTCAAGACGTTCCTAGGGCGGCCGTGGAAGGAATACTCAAGGACGGTTTATATGCAGAGCTTTATGGATGATTTGATTGATCCGGAGGGATGGAGGGCTTGGAATGGGGATTTTGCTTTGAACACTTCGTATTATGCTGAATTTGGGAATTTTGGGCCGGGATCTAACACGTCGGAGAGGGTTAATTGGCCGGGATTTCATTTGATTAATGATACCGATGCTGGCAATTTCACGGCGGGGAATTTTGTGTTGGCCGACGATTGGCTGCCGCAGACCGGCGTTCCCTATACCAGCGGCCTCACGGACTAGAAAACAGGGCAAATTTGTAATTTCATGTTTACTTTTTATTTTTATTTTTTTAAAATTCCAAGGCCAAAGTGTATAGCTATGGAATGAATATGATTGATTCTTTTGTACGGTGATTGATTCTCAAATATAATAAAAATATATTATTTTTCCTTTTT

mRNA sequence

ATTAAACTTCCCTCCTCTCTCCAATGGCTGCCAGAACAACCACCGCCGCCGCCTTCTACTTTCTCATCCTCATTTCCCTTTACATTTCCACTTCCGCCGCCCCCACCGGTTCAGTATGCAGCTCGACTCGAGACCCATCCTACTGCAAATCGGTTCTCCCGAATCAAACCGGCAATGTCTACTCGTACGGACGGTCGTCGTTCAGAAAGTCACTGTCGTCGTCGCAGAAATTCCTCCGGCTGGTGGAGAAATACCTCAGGTCCCGATCGTCGCTGACAGTGCCGGCGGTGAGGGCGCTGGAGGACTGCCAGCTGCTGGCGGGACTGAACATTGATTACTTAACGACGTCGTTCCGGGCGGTGAACTCGACGAGCAGAACGTTGCCGGAGATGAAGGCGGACGACGTGCAGAGTCTGCTGAGTGCGATATTGACGAATCAACAGACTTGTTTGGATGGAATTAAGGCGACGGCGGGTTCTTGGTCGCTCAAAAATGGCCTTTCTCTCCCACTTGCTGCTGACACCAAGCTCTACTCTCTGTCCTTGGCCTTCTTTACCAAAGGTTGGGTTCCAAAGAAGAAGAAAAAACCGACATGGAAAGCCGCCGGAAGACAAGGGGGGTTCAGAAATGGGCGAATGTCACTCAAAATGTCCAGCCGAACCAAAGCGATCTACGAGACGGCGACCCGCCGGAATCTGTTGCAGACCGATGACGGCGGCGACGACCAGATTGCGGTCAGGGACATCGTCGTTGTCAGTCAAGACGGCAGCGGAAATTTCACCACCATCAATGAGGCGATTTCGGCGGCGCCGAACAACTCTGCTCCGTCCGACGGGTATTTCTTGATCTTCGTGTCGGCCGGAATTTATGAAGAATATGTGTCGATTGCGAAGAACAAGAGGTACTTGATGATGATCGGAGATGGCATTAATCAGACCGTCGTCACCGGTAATCGGAGTGTCGTTGACGGCTGGACCACCTTCAATTCCGCCACGTTTGCGGTGGTCGGACCTGGATTCGTAGCAGTAAACATGACATTCAGAAACACCGCCGGCGCCATAAAGCATCAAGCCGTAGCCGTCCGGAATGGCGCCGACTTATCAACATTCTACCTCTGCAGCTTCGAAGCCTACCAAGACACCTTATACACCCATTCCCTCCGCCAATTCTACCGCGACTGCGACATCTACGGCACCGTCGACTTCATCTTCGGCAACGCCGCCGTCGTCTTCCAGAACTGCAACATTTACCCACGCCTCCCGATGTCCAACCAGTTCAACGCCATCACCGCCCAGGGCCGGACCGACCCAAATCAGAACACCGGCACCTCCATCTACAACTGCCGAATCACGGCAGCCGACGATCTGGCCAACAACAGCGGCGCCGGCGTCAAGACGTTCCTAGGGCGGCCGTGGAAGGAATACTCAAGGACGGTTTATATGCAGAGCTTTATGGATGATTTGATTGATCCGGAGGGATGGAGGGCTTGGAATGGGGATTTTGCTTTGAACACTTCGTATTATGCTGAATTTGGGAATTTTGGGCCGGGATCTAACACGTCGGAGAGGGTTAATTGGCCGGGATTTCATTTGATTAATGATACCGATGCTGGCAATTTCACGGCGGGGAATTTTGTGTTGGCCGACGATTGGCTGCCGCAGACCGGCGTTCCCTATACCAGCGGCCTCACGGACTAGAAAACAGGGCAAATTTGTAATTTCATGTTTACTTTTTATTTTTATTTTTTTAAAATTCCAAGGCCAAAGTGTATAGCTATGGAATGAATATGATTGATTCTTTTGTACGGTGATTGATTCTCAAATATAATAAAAATATATTATTTTTCCTTTTT

Coding sequence (CDS)

ATGGCTGCCAGAACAACCACCGCCGCCGCCTTCTACTTTCTCATCCTCATTTCCCTTTACATTTCCACTTCCGCCGCCCCCACCGGTTCAGTATGCAGCTCGACTCGAGACCCATCCTACTGCAAATCGGTTCTCCCGAATCAAACCGGCAATGTCTACTCGTACGGACGGTCGTCGTTCAGAAAGTCACTGTCGTCGTCGCAGAAATTCCTCCGGCTGGTGGAGAAATACCTCAGGTCCCGATCGTCGCTGACAGTGCCGGCGGTGAGGGCGCTGGAGGACTGCCAGCTGCTGGCGGGACTGAACATTGATTACTTAACGACGTCGTTCCGGGCGGTGAACTCGACGAGCAGAACGTTGCCGGAGATGAAGGCGGACGACGTGCAGAGTCTGCTGAGTGCGATATTGACGAATCAACAGACTTGTTTGGATGGAATTAAGGCGACGGCGGGTTCTTGGTCGCTCAAAAATGGCCTTTCTCTCCCACTTGCTGCTGACACCAAGCTCTACTCTCTGTCCTTGGCCTTCTTTACCAAAGGTTGGGTTCCAAAGAAGAAGAAAAAACCGACATGGAAAGCCGCCGGAAGACAAGGGGGGTTCAGAAATGGGCGAATGTCACTCAAAATGTCCAGCCGAACCAAAGCGATCTACGAGACGGCGACCCGCCGGAATCTGTTGCAGACCGATGACGGCGGCGACGACCAGATTGCGGTCAGGGACATCGTCGTTGTCAGTCAAGACGGCAGCGGAAATTTCACCACCATCAATGAGGCGATTTCGGCGGCGCCGAACAACTCTGCTCCGTCCGACGGGTATTTCTTGATCTTCGTGTCGGCCGGAATTTATGAAGAATATGTGTCGATTGCGAAGAACAAGAGGTACTTGATGATGATCGGAGATGGCATTAATCAGACCGTCGTCACCGGTAATCGGAGTGTCGTTGACGGCTGGACCACCTTCAATTCCGCCACGTTTGCGGTGGTCGGACCTGGATTCGTAGCAGTAAACATGACATTCAGAAACACCGCCGGCGCCATAAAGCATCAAGCCGTAGCCGTCCGGAATGGCGCCGACTTATCAACATTCTACCTCTGCAGCTTCGAAGCCTACCAAGACACCTTATACACCCATTCCCTCCGCCAATTCTACCGCGACTGCGACATCTACGGCACCGTCGACTTCATCTTCGGCAACGCCGCCGTCGTCTTCCAGAACTGCAACATTTACCCACGCCTCCCGATGTCCAACCAGTTCAACGCCATCACCGCCCAGGGCCGGACCGACCCAAATCAGAACACCGGCACCTCCATCTACAACTGCCGAATCACGGCAGCCGACGATCTGGCCAACAACAGCGGCGCCGGCGTCAAGACGTTCCTAGGGCGGCCGTGGAAGGAATACTCAAGGACGGTTTATATGCAGAGCTTTATGGATGATTTGATTGATCCGGAGGGATGGAGGGCTTGGAATGGGGATTTTGCTTTGAACACTTCGTATTATGCTGAATTTGGGAATTTTGGGCCGGGATCTAACACGTCGGAGAGGGTTAATTGGCCGGGATTTCATTTGATTAATGATACCGATGCTGGCAATTTCACGGCGGGGAATTTTGTGTTGGCCGACGATTGGCTGCCGCAGACCGGCGTTCCCTATACCAGCGGCCTCACGGACTAG

Protein sequence

MAARTTTAAAFYFLILISLYISTSAAPTGSVCSSTRDPSYCKSVLPNQTGNVYSYGRSSFRKSLSSSQKFLRLVEKYLRSRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRAVNSTSRTLPEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSLPLAADTKLYSLSLAFFTKGWVPKKKKKPTWKAAGRQGGFRNGRMSLKMSSRTKAIYETATRRNLLQTDDGGDDQIAVRDIVVVSQDGSGNFTTINEAISAAPNNSAPSDGYFLIFVSAGIYEEYVSIAKNKRYLMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQFNAITAQGRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQSFMDDLIDPEGWRAWNGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGNFVLADDWLPQTGVPYTSGLTD
Homology
BLAST of CaUC09G163230 vs. NCBI nr
Match: XP_038896831.1 (probable pectinesterase/pectinesterase inhibitor 7 [Benincasa hispida])

HSP 1 Score: 1076.6 bits (2783), Expect = 0.0e+00
Identity = 540/561 (96.26%), Postives = 550/561 (98.04%), Query Frame = 0

Query: 1   MAART----TTAAAFYFLILISLYISTSAAPTGSVCSSTRDPSYCKSVLPNQTGNVYSYG 60
           MAA T    T AAAFYFLILISLYI+TSAAPTGS CSSTRDPSYCKSVLPNQTGNVYSYG
Sbjct: 1   MAAGTIPAATAAAAFYFLILISLYITTSAAPTGSECSSTRDPSYCKSVLPNQTGNVYSYG 60

Query: 61  RSSFRKSLSSSQKFLRLVEKYLRSRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRAVNST 120
           RSSFRKSLS+SQKFLRLVEK+LRSRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRAVNST
Sbjct: 61  RSSFRKSLSASQKFLRLVEKHLRSRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRAVNST 120

Query: 121 SRTLPEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSLPLAADTKLYSLSLAF 180
           SRTLPEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSLPLAADTKLYSLSLAF
Sbjct: 121 SRTLPEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSLPLAADTKLYSLSLAF 180

Query: 181 FTKGWVPKKKKKPTWKAAGRQGGFRNGRMSLKMSSRTKAIYETATRRNLLQTDDGGDDQI 240
           FTKGWVPKKKKKPTWKAAGR GGFRNGRMSLKMSSR KAIYETATRRNLLQ DDGGDDQI
Sbjct: 181 FTKGWVPKKKKKPTWKAAGRHGGFRNGRMSLKMSSRAKAIYETATRRNLLQADDGGDDQI 240

Query: 241 AVRDIVVVSQDGSGNFTTINEAISAAPNNSAPSDGYFLIFVSAGIYEEYVSIAKNKRYLM 300
           AVRDIVVVSQDGSGNFTTINEA++AAPNNSAP+DGYFLIFVSAG+YEEYVSIAKNKRYLM
Sbjct: 241 AVRDIVVVSQDGSGNFTTINEAVAAAPNNSAPTDGYFLIFVSAGVYEEYVSIAKNKRYLM 300

Query: 301 MIGDGINQTVVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNG 360
           MIGDGINQTVVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNG
Sbjct: 301 MIGDGINQTVVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNG 360

Query: 361 ADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSN 420
           ADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSN
Sbjct: 361 ADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSN 420

Query: 421 QFNAITAQGRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQSF 480
           QFNAITAQGRTDPNQNTGTSIYNCRITAADDLANN+GAGVKTFLGRPWKEYSRTVYMQSF
Sbjct: 421 QFNAITAQGRTDPNQNTGTSIYNCRITAADDLANNTGAGVKTFLGRPWKEYSRTVYMQSF 480

Query: 481 MDDLIDPEGWRAWNGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGN 540
           MDDLI+P GWRAW+GDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGN
Sbjct: 481 MDDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGN 540

Query: 541 FVLADDWLPQTGVPYTSGLTD 558
           FVLADDWLPQTGVPYTSGLTD
Sbjct: 541 FVLADDWLPQTGVPYTSGLTD 561

BLAST of CaUC09G163230 vs. NCBI nr
Match: XP_008451544.1 (PREDICTED: probable pectinesterase/pectinesterase inhibitor 7 [Cucumis melo] >KAA0046659.1 putative pectinesterase/pectinesterase inhibitor 7 [Cucumis melo var. makuwa] >TYK18194.1 putative pectinesterase/pectinesterase inhibitor 7 [Cucumis melo var. makuwa])

HSP 1 Score: 1051.6 bits (2718), Expect = 2.4e-303
Identity = 527/557 (94.61%), Postives = 543/557 (97.49%), Query Frame = 0

Query: 3   ARTTTAAAFYFLILISLYISTSAAPTGSVCSSTRDPSYCKSVLPNQTGNVYSYGRSSFRK 62
           A  TTAAAFY LILISLYI+TSA+PTGSVCSST DPSYCKS LPNQTGNVYSYGRSSFRK
Sbjct: 2   AAGTTAAAFYVLILISLYITTSASPTGSVCSSTPDPSYCKSALPNQTGNVYSYGRSSFRK 61

Query: 63  SLSSSQKFLRLVEKYLRSRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRAVNSTSRTLPE 122
           SLSSSQKFLRLVEK+LRSRSSLTV AVRALEDCQLLAGLNIDYL TSF+AVN+TSR L E
Sbjct: 62  SLSSSQKFLRLVEKHLRSRSSLTVAAVRALEDCQLLAGLNIDYLRTSFQAVNATSRALTE 121

Query: 123 MKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSLPLAADTKLYSLSLAFFTKGWV 182
           MKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLS PLA+DTKLYSLSLAFFTKGWV
Sbjct: 122 MKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSQPLASDTKLYSLSLAFFTKGWV 181

Query: 183 PKKKKKPTWKAAGRQGGFRNGRMSLKMSSRTKAIYETATRRNLLQTDD--GGDDQIAVRD 242
           PKKKKKPTWKAAGRQGGFRNGRMSLKMSSRT+AIYETATRRNLLQTDD  G DDQIAVRD
Sbjct: 182 PKKKKKPTWKAAGRQGGFRNGRMSLKMSSRTQAIYETATRRNLLQTDDDGGDDDQIAVRD 241

Query: 243 IVVVSQDGSGNFTTINEAISAAPNNSAPSDGYFLIFVSAGIYEEYVSIAKNKRYLMMIGD 302
           IVVVSQDG+GNFTTINEAI+AAPNNSAP+DGYFLIFVSAG+YEEYVSIAKNKRYLMMIGD
Sbjct: 242 IVVVSQDGTGNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVYEEYVSIAKNKRYLMMIGD 301

Query: 303 GINQTVVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGADLS 362
           GINQT+VTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVA+RNGADLS
Sbjct: 302 GINQTIVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVALRNGADLS 361

Query: 363 TFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQFNA 422
           TFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQFNA
Sbjct: 362 TFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQFNA 421

Query: 423 ITAQGRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQSFMDDL 482
           ITAQGRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQSFMDDL
Sbjct: 422 ITAQGRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQSFMDDL 481

Query: 483 IDPEGWRAWNGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGNFVLA 542
           I+P GWRAW+GDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGNFVLA
Sbjct: 482 INPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGNFVLA 541

Query: 543 DDWLPQTGVPYTSGLTD 558
           DDWLPQTGVPYTSGLT+
Sbjct: 542 DDWLPQTGVPYTSGLTE 558

BLAST of CaUC09G163230 vs. NCBI nr
Match: XP_004136013.1 (probable pectinesterase/pectinesterase inhibitor 7 [Cucumis sativus] >KGN44976.1 hypothetical protein Csa_016133 [Cucumis sativus])

HSP 1 Score: 1030.8 bits (2664), Expect = 4.4e-297
Identity = 522/560 (93.21%), Postives = 538/560 (96.07%), Query Frame = 0

Query: 1   MAARTT-TAAAFY-FLILISLYISTSAAPTGSVCSSTRDPSYCKSVLPNQTGNVYSYGRS 60
           MAA TT TAAAFY  LILISLYI+TSA+PTGSVCSST DPSYCKS LPNQTGNVYSYGRS
Sbjct: 1   MAAGTTATAAAFYVLLILISLYITTSASPTGSVCSSTPDPSYCKSALPNQTGNVYSYGRS 60

Query: 61  SFRKSLSSSQKFLRLVEKYLRSRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRAVNSTSR 120
           SFRKSLSSSQKFLRLVEK+LRSRSSLTVPAVRALEDC LLAGLNIDYL TSF+ VN+TSR
Sbjct: 61  SFRKSLSSSQKFLRLVEKHLRSRSSLTVPAVRALEDCLLLAGLNIDYLKTSFQTVNTTSR 120

Query: 121 TLPEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSLPLAADTKLYSLSLAFFT 180
            L EMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLS PLA+DTKLYSLSLAFFT
Sbjct: 121 VLTEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSQPLASDTKLYSLSLAFFT 180

Query: 181 KGWVPKKKKKPTWKAAGRQGGFRNGRMSLKMSSRTKAIYETATRRNLLQTDDGG-DDQIA 240
           KGWVPKKKK+PTWKAAGRQGGFRNGRMSLKMSSRT+AIYE ATRRNLLQTDDGG DDQI 
Sbjct: 181 KGWVPKKKKRPTWKAAGRQGGFRNGRMSLKMSSRTQAIYEKATRRNLLQTDDGGDDDQIK 240

Query: 241 VRDIVVVSQDGSGNFTTINEAISAAPNNSAPSDGYFLIFVSAGIYEEYVSIAKNKRYLMM 300
           VRDIVVVSQDGSGNFTTINEAI+AA NNSAP+DGYFLIFVSAG+YEEYV +AKNKRYLMM
Sbjct: 241 VRDIVVVSQDGSGNFTTINEAIAAATNNSAPTDGYFLIFVSAGVYEEYVLVAKNKRYLMM 300

Query: 301 IGDGINQTVVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGA 360
           IGDGINQT+VTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGA
Sbjct: 301 IGDGINQTIVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGA 360

Query: 361 DLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQ 420
           DLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQ
Sbjct: 361 DLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQ 420

Query: 421 FNAITAQGRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQSFM 480
           FNAITAQGRTDPNQNTGTSIYNCRITAADDLANNS AGVKTFLGRPWKEYSRTVYMQSFM
Sbjct: 421 FNAITAQGRTDPNQNTGTSIYNCRITAADDLANNSDAGVKTFLGRPWKEYSRTVYMQSFM 480

Query: 481 DDLIDPEGWRAWNGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGNF 540
           DDLI+P GWRAW+GDFALNTSYYAEFGNFGPGSNTSERV W GFHLINDTDAGNFTAGNF
Sbjct: 481 DDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVTWAGFHLINDTDAGNFTAGNF 540

Query: 541 VLADDWLPQTGVPYTSGLTD 558
           VLADDWLPQTGVPY SGLT+
Sbjct: 541 VLADDWLPQTGVPYDSGLTE 560

BLAST of CaUC09G163230 vs. NCBI nr
Match: KAG7014346.1 (putative pectinesterase/pectinesterase inhibitor 41, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1024.2 bits (2647), Expect = 4.1e-295
Identity = 513/562 (91.28%), Postives = 534/562 (95.02%), Query Frame = 0

Query: 1   MAARTTTAAAFYFLILISLY-----ISTSAAPTGSVCSSTRDPSYCKSVLPNQTGNVYSY 60
           MAA   T AAFY L+LISLY     ISTSAAPTGSVCSS  DPSYCKS LPNQTGNVYSY
Sbjct: 1   MAAAPPTTAAFYSLLLISLYITAAGISTSAAPTGSVCSSAPDPSYCKSALPNQTGNVYSY 60

Query: 61  GRSSFRKSLSSSQKFLRLVEKYLRSRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRAVNS 120
           GRSS RKSLSSSQKFLRLVEKYLRSRSSLTV AVRALEDCQLLAGLNIDYLTTSFR VNS
Sbjct: 61  GRSSVRKSLSSSQKFLRLVEKYLRSRSSLTVAAVRALEDCQLLAGLNIDYLTTSFRTVNS 120

Query: 121 TSRTLPEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSLPLAADTKLYSLSLA 180
           TS TLPEMKA+DVQSLLSAILTNQQTCLDG+KATAGSWSLKNGLSLPLAADTKLYS+SLA
Sbjct: 121 TSWTLPEMKAEDVQSLLSAILTNQQTCLDGLKATAGSWSLKNGLSLPLAADTKLYSMSLA 180

Query: 181 FFTKGWVPKKKKKPTWKAAGRQGGFRNGRMSLKMSSRTKAIYETATRRNLLQTDDGGDDQ 240
           FFTKGWVPKKKKKPTW +AGRQGGF NGRMSLKMSSRTKAIYETATRRNLLQTDDGGDDQ
Sbjct: 181 FFTKGWVPKKKKKPTWTSAGRQGGFSNGRMSLKMSSRTKAIYETATRRNLLQTDDGGDDQ 240

Query: 241 IAVRDIVVVSQDGSGNFTTINEAISAAPNNSAPSDGYFLIFVSAGIYEEYVSIAKNKRYL 300
           I+VRDIVVVSQDGSGNFTTINEAI+AAPNNSAP+DGYFLIFV AG+YEEYV+IAKNKRYL
Sbjct: 241 ISVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVDAGVYEEYVTIAKNKRYL 300

Query: 301 MMIGDGINQTVVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRN 360
           MMIGDGINQTVVTGNR+VVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRN
Sbjct: 301 MMIGDGINQTVVTGNRNVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRN 360

Query: 361 GADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMS 420
            ADLSTFYLCSFE YQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVV QNCNIYPRLPM+
Sbjct: 361 SADLSTFYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVLQNCNIYPRLPMA 420

Query: 421 NQFNAITAQGRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQS 480
           NQFNAITAQGRTDPNQNTGTSI+NCRITAADDLANNSGA V TFLGRPWKEYSRTVYMQS
Sbjct: 421 NQFNAITAQGRTDPNQNTGTSIHNCRITAADDLANNSGARVNTFLGRPWKEYSRTVYMQS 480

Query: 481 FMDDLIDPEGWRAWNGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAG 540
           FMDDLI+P GWRAW+GDFALNT YYAEFGNFGPGSNT+ERVNW GFHLI+DTDAGNFTAG
Sbjct: 481 FMDDLINPAGWRAWDGDFALNTLYYAEFGNFGPGSNTTERVNWVGFHLIDDTDAGNFTAG 540

Query: 541 NFVLADDWLPQTGVPYTSGLTD 558
           NF++ADDW+PQTGVPYTSGLT+
Sbjct: 541 NFLMADDWVPQTGVPYTSGLTE 562

BLAST of CaUC09G163230 vs. NCBI nr
Match: KAG6575804.1 (putative pectinesterase/pectinesterase inhibitor 41, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1023.8 bits (2646), Expect = 5.4e-295
Identity = 513/562 (91.28%), Postives = 534/562 (95.02%), Query Frame = 0

Query: 1   MAARTTTAAAFYFLILISLY-----ISTSAAPTGSVCSSTRDPSYCKSVLPNQTGNVYSY 60
           MAA   T AAFY L+LISLY     ISTSAAPTGSVCSS  DPSYCKS LPNQTGNVYSY
Sbjct: 1   MAAAPPTTAAFYSLLLISLYITAAGISTSAAPTGSVCSSAPDPSYCKSALPNQTGNVYSY 60

Query: 61  GRSSFRKSLSSSQKFLRLVEKYLRSRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRAVNS 120
           GRSS RKSLSSSQKFLRLVEKYLRSRSSLTV AVRALEDCQLLAGLNIDYLTTSFR VNS
Sbjct: 61  GRSSVRKSLSSSQKFLRLVEKYLRSRSSLTVAAVRALEDCQLLAGLNIDYLTTSFRTVNS 120

Query: 121 TSRTLPEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSLPLAADTKLYSLSLA 180
           TS TLPEMKA+DVQSLLSAILTNQQTCLDG+KATAGSWSLKNGLSLPLAADTKLYS+SLA
Sbjct: 121 TSWTLPEMKAEDVQSLLSAILTNQQTCLDGLKATAGSWSLKNGLSLPLAADTKLYSMSLA 180

Query: 181 FFTKGWVPKKKKKPTWKAAGRQGGFRNGRMSLKMSSRTKAIYETATRRNLLQTDDGGDDQ 240
           FFTKGWVPKKKKKPTW +AGRQGGF NGRMSLKMSSRTKAIYETATRRNLLQT+DGGDDQ
Sbjct: 181 FFTKGWVPKKKKKPTWTSAGRQGGFSNGRMSLKMSSRTKAIYETATRRNLLQTEDGGDDQ 240

Query: 241 IAVRDIVVVSQDGSGNFTTINEAISAAPNNSAPSDGYFLIFVSAGIYEEYVSIAKNKRYL 300
           I+VRDIVVVSQDGSGNFTTINEAI+AAPNNSAP+DGYFLIFV AG+YEEYV+IAKNKRYL
Sbjct: 241 ISVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVDAGVYEEYVTIAKNKRYL 300

Query: 301 MMIGDGINQTVVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRN 360
           MMIGDGINQTVVTGNR+VVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRN
Sbjct: 301 MMIGDGINQTVVTGNRNVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRN 360

Query: 361 GADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMS 420
            ADLSTFYLCSFE YQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVV QNCNIYPRLPM+
Sbjct: 361 SADLSTFYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVLQNCNIYPRLPMA 420

Query: 421 NQFNAITAQGRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQS 480
           NQFNAITAQGRTDPNQNTGTSI+NCRITAADDLANNSGA V TFLGRPWKEYSRTVYMQS
Sbjct: 421 NQFNAITAQGRTDPNQNTGTSIHNCRITAADDLANNSGARVNTFLGRPWKEYSRTVYMQS 480

Query: 481 FMDDLIDPEGWRAWNGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAG 540
           FMDDLI+P GWRAW+GDFALNT YYAEFGNFGPGSNTSERVNW GFHLI+DTDAGNFTAG
Sbjct: 481 FMDDLINPAGWRAWDGDFALNTLYYAEFGNFGPGSNTSERVNWVGFHLIDDTDAGNFTAG 540

Query: 541 NFVLADDWLPQTGVPYTSGLTD 558
           NF++ADDW+PQTGVPYTSGLT+
Sbjct: 541 NFLMADDWVPQTGVPYTSGLTE 562

BLAST of CaUC09G163230 vs. ExPASy Swiss-Prot
Match: Q9SRX4 (Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PME7 PE=2 SV=1)

HSP 1 Score: 706.8 bits (1823), Expect = 1.9e-202
Identity = 357/573 (62.30%), Postives = 433/573 (75.57%), Query Frame = 0

Query: 12  YFLILISLYI------STSAAPTGSVCSSTRDPSYCKSVLPNQTGNVYSYGRSSFRKSLS 71
           + LI +S ++      S + + + ++C +T DP YCKSV P+  GNV  YG  S RKSLS
Sbjct: 6   FILITLSFFLQSVLASSQTLSNSSTICKTTPDPKYCKSVFPHSQGNVQQYGCFSIRKSLS 65

Query: 72  SSQKFLRLVEKYLRSRSSLTVPAV-RALEDCQLLAGLNIDYLTTSFRAVNSTS-----RT 131
            S+KF+R V++Y++  + L+ PAV RAL+DC+ LAGL +DYL TSF  VN TS     + 
Sbjct: 66  QSRKFIRTVDRYIKRNAHLSQPAVIRALQDCRFLAGLTMDYLLTSFETVNDTSAKTSFKP 125

Query: 132 LPEMKADDVQSLLSAILTNQQTCLDGIKATA---GSWSLKNGLSLPLAADTKLYSLSLAF 191
           L   KADD+Q+LLSA LTN+QTCL+G+   A    +W+++ G++LPL  DTKL  +SLA 
Sbjct: 126 LSFPKADDIQTLLSAALTNEQTCLEGLTTAASYSATWTVRTGVALPLVNDTKLLGVSLAL 185

Query: 192 FTKGWVPKKKKKPTWKAAGRQGG----------FRNGRMSLKMSSRTKAIYETATRRNLL 251
           FTKGWVPKKKK+  +  A  + G          FRNG + LKM+ +TKA+YE+ +RR L 
Sbjct: 186 FTKGWVPKKKKRAGFAWAQPRSGSSTHTKPFRLFRNGALPLKMTEKTKAVYESLSRRKLA 245

Query: 252 QTDDGGD----DQIAVRDIVVVSQDGSGNFTTINEAISAAPNNSAPSDGYFLIFVSAGIY 311
             D  GD      + + DIV VSQDG+GNFT I  A++AAPNN+  S G+FLI+V+AGIY
Sbjct: 246 DGDSNGDGDDGSMVLISDIVTVSQDGTGNFTNITAAVAAAPNNTDGSAGFFLIYVTAGIY 305

Query: 312 EEYVSIAKNKRYLMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNT 371
           EEY+SIAKNKRY+MMIGDGINQTVVTGNRSVVDGWTTFNSATFAV  P FVAVN+TFRNT
Sbjct: 306 EEYISIAKNKRYMMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVTAPNFVAVNITFRNT 365

Query: 372 AGAIKHQAVAVRNGADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVV 431
           AG  KHQAVA+R+GAD S FY CSFEAYQDTLYTHSLRQFYR+CD+YGTVDFIFGNAAVV
Sbjct: 366 AGPEKHQAVALRSGADFSIFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVV 425

Query: 432 FQNCNIYPRLPMSNQFNAITAQGRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGR 491
           FQNCN+YPR PM NQFNAITAQGR+DPNQNTGTSI NC I  ADDL  +S   VKT+LGR
Sbjct: 426 FQNCNLYPRKPMPNQFNAITAQGRSDPNQNTGTSIQNCTIKPADDLV-SSNYTVKTYLGR 485

Query: 492 PWKEYSRTVYMQSFMDDLIDPEGWRAWNGDFALNTSYYAEFGNFGPGSNTSERVNWPGFH 551
           PWKEYSRTVYMQS++D  ++P GWR WNGDFAL+T YYAE+ N GPGSNT+ RV WPG+H
Sbjct: 486 PWKEYSRTVYMQSYIDGFVEPVGWREWNGDFALSTLYYAEYNNTGPGSNTTNRVTWPGYH 545

Query: 552 LINDTDAGNFTAGNFVLADDWLPQTGVPYTSGL 556
           +IN TDA NFT     +  DW+ +TGVPYTSGL
Sbjct: 546 VINSTDAANFTVTGLFIEADWIWKTGVPYTSGL 577

BLAST of CaUC09G163230 vs. ExPASy Swiss-Prot
Match: Q8RXK7 (Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana OX=3702 GN=PME41 PE=2 SV=2)

HSP 1 Score: 684.9 bits (1766), Expect = 7.7e-196
Identity = 354/562 (62.99%), Postives = 434/562 (77.22%), Query Frame = 0

Query: 13  FLILISLYI-STSAAPTGS---VCSSTRDPSYCKSVLPNQT-GNVYSYGRSSFRKSLSSS 72
           FL L +L+I S +  P+ S   +C +T DP +CKSV P  + G+V  YGR S RKSL+ S
Sbjct: 12  FLSLQTLFIASQTLLPSNSSSTICKTTPDPKFCKSVFPQTSQGDVREYGRFSLRKSLTQS 71

Query: 73  QKFLRLVEKYL-RSRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRAVNST--SRTLPEMK 132
           +KF R +++YL R+ + L+  AV AL+DC+ LA L  DYL TSF  VN T  S+TL   K
Sbjct: 72  RKFTRTIDRYLKRNNALLSQSAVGALQDCRYLASLTTDYLITSFETVNITTSSKTLSFSK 131

Query: 133 ADDVQSLLSAILTNQQTCLDGIK-ATAGSWSLKNGLSLPLAADTKLYSLSLAFFTKGWVP 192
           AD++Q+LLSA LTN+QTCLDGI  A + SW+++NG++LPL  DTKL+S+SLA FTKGWVP
Sbjct: 132 ADEIQTLLSAALTNEQTCLDGINTAASSSWTIRNGVALPLINDTKLFSVSLALFTKGWVP 191

Query: 193 KKKK----------KPTWKAAGRQGGFRNGRMSLKMSSRTKAIYETATRRNLLQTDDGGD 252
           KKKK          K T         FRNG + LKM+  T+A+YE+ +RR L   DD   
Sbjct: 192 KKKKQVASYSWAHPKNTHSHTKPFRHFRNGALPLKMTEHTRAVYESLSRRKLAD-DDNDV 251

Query: 253 DQIAVRDIVVVSQDGSGNFTTINEAISAAPNNSAPSDGYFLIFVSAGIYEEYVSIAKNKR 312
           + + V DIV V+Q+G+GNFTTI EA+++APN +  + GYF+I+V++G+YEE V IAKNKR
Sbjct: 252 NTVLVSDIVTVNQNGTGNFTTITEAVNSAPNKTDGTAGYFVIYVTSGVYEENVVIAKNKR 311

Query: 313 YLMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAV 372
           YLMMIGDGIN+TVVTGNR+VVDGWTTFNSATFAV  P FVAVNMTFRNTAG  KHQAVA+
Sbjct: 312 YLMMIGDGINRTVVTGNRNVVDGWTTFNSATFAVTSPNFVAVNMTFRNTAGPEKHQAVAM 371

Query: 373 RNGADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLP 432
           R+ ADLS FY CSFEAYQDTLYTHSLRQFYR+CDIYGTVDFIFGNAAVVFQ+CN+YPR P
Sbjct: 372 RSSADLSIFYSCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQDCNLYPRQP 431

Query: 433 MSNQFNAITAQGRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYM 492
           M NQFNAITAQGRTDPNQNTG SI+NC I  ADDL  +S   VKT+LGRPWKEYSRTV+M
Sbjct: 432 MQNQFNAITAQGRTDPNQNTGISIHNCTIKPADDLV-SSNYTVKTYLGRPWKEYSRTVFM 491

Query: 493 QSFMDDLIDPEGWRAWNGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFT 552
           QS++D++++P GWR WNGDFAL+T YYAE+ N G GS+T++RV WPG+H+IN TDA NFT
Sbjct: 492 QSYIDEVVEPVGWREWNGDFALSTLYYAEYNNTGSGSSTTDRVVWPGYHVINSTDANNFT 551

Query: 553 AGNFVLADDWLPQTGVPYTSGL 556
             NF+L D W+ Q+GVPY SGL
Sbjct: 552 VENFLLGDGWMVQSGVPYISGL 571

BLAST of CaUC09G163230 vs. ExPASy Swiss-Prot
Match: O22256 (Probable pectinesterase/pectinesterase inhibitor 20 OS=Arabidopsis thaliana OX=3702 GN=PME20 PE=2 SV=2)

HSP 1 Score: 657.5 bits (1695), Expect = 1.3e-187
Identity = 324/537 (60.34%), Postives = 411/537 (76.54%), Query Frame = 0

Query: 24  SAAPTGSVCSSTRDPSYCKSVLPNQTGNVYSYGRSSFRKSLSSSQKFLRLVEKYLRSRSS 83
           +A    ++C    DPSYC+SVLPNQ G++YSYGR S R+SLS +++F+ +++  L  +  
Sbjct: 31  NATSPSNICRFAPDPSYCRSVLPNQPGDIYSYGRLSLRRSLSRARRFISMIDAELDRKGK 90

Query: 84  LTVPA-VRALEDCQLLAGLNIDYLTTSFRAVNSTSRTLPEMKADDVQSLLSAILTNQQTC 143
           +   + V ALEDC+ LA L +DYL +S +  +ST +TL   +A+DV + LSA +TN+QTC
Sbjct: 91  VAAKSTVGALEDCKFLASLTMDYLLSSSQTADST-KTLSLSRAEDVHTFLSAAITNEQTC 150

Query: 144 LDGIKATAGSWSLKNGLSLPLAADTKLYSLSLAFFTKGWVPKKKK-KPTWKAAGRQG--- 203
           L+G+K+TA     +NGLS  L  DTKLY +SLA F+KGWVP++++ +P W+   R     
Sbjct: 151 LEGLKSTAS----ENGLSGDLFNDTKLYGVSLALFSKGWVPRRQRSRPIWQPQARFKKFF 210

Query: 204 GFRNGRMSLKMSSRTKAIYETATRRNLLQTDDGGDDQIAVRDIVVVSQDGSGNFTTINEA 263
           GFRNG++ LKM+ R +A+Y T TRR LLQ+D    D + V DIV V Q+G+GNFTTIN A
Sbjct: 211 GFRNGKLPLKMTERARAVYNTVTRRKLLQSD---ADAVQVSDIVTVIQNGTGNFTTINAA 270

Query: 264 ISAAPNNSAPSDGYFLIFVSAGIYEEYVSIAKNKRYLMMIGDGINQTVVTGNRSVVDGWT 323
           I+AAPN +  S+GYFLI+V+AG+YEEYV + KNKRY+MMIGDGINQTV+TGNRSVVDGWT
Sbjct: 271 IAAAPNKTDGSNGYFLIYVTAGLYEEYVEVPKNKRYVMMIGDGINQTVITGNRSVVDGWT 330

Query: 324 TFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGADLSTFYLCSFEAYQDTLYTHS 383
           TFNSATF + GP F+ VN+T RNTAG  K QAVA+R+G DLS FY CSFEAYQDTLYTHS
Sbjct: 331 TFNSATFILSGPNFIGVNITIRNTAGPTKGQAVALRSGGDLSVFYSCSFEAYQDTLYTHS 390

Query: 384 LRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQFNAITAQGRTDPNQNTGTSIY 443
           LRQFYR+CD+YGTVDFIFGNAAVV QNCN+YPR P   Q N +TAQGRTDPNQNTGT+I+
Sbjct: 391 LRQFYRECDVYGTVDFIFGNAAVVLQNCNLYPRQPRKGQSNEVTAQGRTDPNQNTGTAIH 450

Query: 444 NCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQSFMDDLIDPEGWRAWNGDFALNTS 503
            C I  ADDLA  S   VKT+LGRPWKEYSRTV MQ+++D  ++P GW AW+GDFAL+T 
Sbjct: 451 GCTIRPADDLA-TSNYTVKTYLGRPWKEYSRTVVMQTYIDGFLEPSGWNAWSGDFALSTL 510

Query: 504 YYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGNFVLADDWLPQTGVPYTSGL 556
           YYAE+ N GPGS+T+ RV WPG+H+IN TDA NFT  NF++ + W+ QTGVP+  GL
Sbjct: 511 YYAEYNNTGPGSDTTNRVTWPGYHVINATDASNFTVTNFLVGEGWIGQTGVPFVGGL 558

BLAST of CaUC09G163230 vs. ExPASy Swiss-Prot
Match: Q94CB1 (Probable pectinesterase/pectinesterase inhibitor 25 OS=Arabidopsis thaliana OX=3702 GN=PME25 PE=2 SV=1)

HSP 1 Score: 485.7 bits (1249), Expect = 6.9e-136
Identity = 260/553 (47.02%), Postives = 351/553 (63.47%), Query Frame = 0

Query: 18  SLYISTSAAPTGSVCSSTRDPSYCKSVL---PNQTGNVYSYGRSSFRKSLSSSQKFLRLV 77
           SL  S S +P+   C ST  P  C+++L    +   + Y YG+ + ++ L  + +  +++
Sbjct: 70  SLPPSQSQSPS-LACKSTPYPKLCRTILNAVKSSPSDPYRYGKFTIKQCLKQASRLSKVI 129

Query: 78  EKYLR----SRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRAVNSTSRTLPEMKADDVQS 137
             Y R       S T   + A+ DC  L+ L+++YL T    +  T++ +     + V S
Sbjct: 130 TSYARRVESKPGSATAEEIGAVADCGELSELSVNYLETVTTEL-KTAQVMTAALVEHVNS 189

Query: 138 LLSAILTNQQTCLDGIKATAGSWSLKNGLSLPLAADTKLYSLSLAFFTKGWVPKKKKKPT 197
           LLS ++TNQQTCLDG+      ++    +  P+   T+LYS+SL   +       K+   
Sbjct: 190 LLSGVVTNQQTCLDGLVEAKSGFAA--AIGSPMGNLTRLYSISLGLVSHALNRNLKRFKA 249

Query: 198 WKAAGRQGGFRNGRMSLKMSSRTKAIYETA--------TRRNLLQTDDGGDDQIAVRDIV 257
            K     GG  N      + +  K + +T         T RNL +  +     I V   V
Sbjct: 250 SKGKILGGG--NSTYREPLETLIKGLRKTCDNDKDCRKTSRNLGELGETSGGSILVSKAV 309

Query: 258 VVSQDGSGNFTTINEAISAAPNNSAPSDGYFLIFVSAGIYEEYVSIAKNKRYLMMIGDGI 317
           +V    S NFTTI +AI+AAPNN+ P DGYF+I+   G+YEEY+ +  NK+ LM++GDGI
Sbjct: 310 IVGPFKSDNFTTITDAIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLMLMGDGI 369

Query: 318 NQTVVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGADLSTF 377
           N+T++TGN +VVDGWTT+N ++FAVVG  F+AV++TFRNTAG  KHQAVA+RN A+ S+F
Sbjct: 370 NKTIITGNHNVVDGWTTYNCSSFAVVGERFMAVDVTFRNTAGPEKHQAVALRNNAEGSSF 429

Query: 378 YLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQFNAIT 437
           Y CSFE YQDTLY HSLRQFYR+CDIYGTVDFIFGNAA +FQNCNIY R PM+ Q NAIT
Sbjct: 430 YRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAIFQNCNIYARKPMAKQKNAIT 489

Query: 438 AQGRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQSFMDDLID 497
           A GR DPNQNTG SI NC I AA DLA    + + TFLGRPWK YSRTV+MQS++ D++ 
Sbjct: 490 AHGRLDPNQNTGISIINCTIKAAPDLAAEPKSAM-TFLGRPWKPYSRTVFMQSYISDIVQ 549

Query: 498 PEGWRAWNGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGNFVLADD 556
           P GW  WNG   L+T YY E+ NFGPG+NT++RV W G++L+N  +A NFT  NF + D 
Sbjct: 550 PVGWLEWNGTIGLDTIYYGEYSNFGPGANTNQRVQWLGYNLLNLAEAMNFTVYNFTMGDT 609

BLAST of CaUC09G163230 vs. ExPASy Swiss-Prot
Match: Q9FF77 (Probable pectinesterase/pectinesterase inhibitor 47 OS=Arabidopsis thaliana OX=3702 GN=PME47 PE=2 SV=1)

HSP 1 Score: 484.6 bits (1246), Expect = 1.5e-135
Identity = 259/547 (47.35%), Postives = 346/547 (63.25%), Query Frame = 0

Query: 24  SAAPTGSVCSSTRDPSYCKSVL---PNQTGNVYSYGRSSFRKSLSSSQKFLRLVEKYLR- 83
           S  P    C ST  P  C+++L    +   + Y YG+ + ++ L  +++  +++ ++ + 
Sbjct: 80  SPLPPNIACKSTPYPKLCRTILSAVKSSPSDPYHYGKFTMKQCLKQARRLSKVINRFAQR 139

Query: 84  ---SRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRAVNSTSRTLPEMKADDVQSLLSAIL 143
                 + TV  V A+ DC  LA L+++YL T    + + +  +     D V SLL  ++
Sbjct: 140 VEADPGTSTVEEVSAVADCGELAELSVEYLETVTEELKA-AELMTAALVDRVTSLLGGVV 199

Query: 144 TNQQTCLDGIKATAGSWSLKNGLSLPLAADTKLYSLSLAFFTKGWVPKKKKKPTWKAAGR 203
           TNQQTCLDG+      ++    +  PL   T+LYS+SL   +       K+    K  G+
Sbjct: 200 TNQQTCLDGLVDAKSGFA--TAIGTPLGNLTRLYSVSLGLVSHALNRNLKRYKGSK--GK 259

Query: 204 QGGFRNGRMSLKMSSRTKAIYETATR--------RNLLQTDDGGDDQIAVRDIVVVSQDG 263
             G  N  +   + +  K + +T  +        RNL +  +     I VR+ V V    
Sbjct: 260 IFGGGNKPVREPLETLIKVLRKTCDKGKDCRKANRNLGELGETSGGSILVREAVTVGPYE 319

Query: 264 SGNFTTINEAISAAPNNSAPSDGYFLIFVSAGIYEEYVSIAKNKRYLMMIGDGINQTVVT 323
           + NF TI EA++AAPN++ P  GYF+I+  AG+YEEYV I+  KR +M+IGDGIN+T+++
Sbjct: 320 TDNFPTITEAVAAAPNHTFPEQGYFVIYARAGLYEEYVVISNKKRNIMLIGDGINKTIIS 379

Query: 324 GNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGADLSTFYLCSFE 383
           GN S +DGWTT+NS+TFAVVG  FVAV++TFRNTAG  KHQAVAVRN AD STFY CSFE
Sbjct: 380 GNHSFIDGWTTYNSSTFAVVGDRFVAVDVTFRNTAGPEKHQAVAVRNNADGSTFYRCSFE 439

Query: 384 AYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQFNAITAQGRTD 443
            YQDTLY HSLRQFYR+CDIYGT+DFIFGNAA +FQNCNIY R PM+NQ NA+TA GRTD
Sbjct: 440 GYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAAIFQNCNIYARKPMANQKNAVTAHGRTD 499

Query: 444 PNQNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQSFMDDLIDPEGWRA 503
           PNQ TG SI NC I AA DLA +      TFLGRPWK YSRTVY+QS++ D++ P GW  
Sbjct: 500 PNQKTGISIINCTIGAAPDLAADP-KSTMTFLGRPWKPYSRTVYIQSYISDVVQPVGWLE 559

Query: 504 WNGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGNFVLADDWLPQTG 556
           WNG   L+T  Y E+ NFGPG++TS+RV W G+ L+N   A NFT  NF L D WLPQT 
Sbjct: 560 WNGTTGLDTISYGEYDNFGPGADTSKRVQWSGYSLLNLVQAMNFTVYNFTLGDTWLPQTD 619

BLAST of CaUC09G163230 vs. ExPASy TrEMBL
Match: A0A5D3D3H4 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G001320 PE=3 SV=1)

HSP 1 Score: 1051.6 bits (2718), Expect = 1.2e-303
Identity = 527/557 (94.61%), Postives = 543/557 (97.49%), Query Frame = 0

Query: 3   ARTTTAAAFYFLILISLYISTSAAPTGSVCSSTRDPSYCKSVLPNQTGNVYSYGRSSFRK 62
           A  TTAAAFY LILISLYI+TSA+PTGSVCSST DPSYCKS LPNQTGNVYSYGRSSFRK
Sbjct: 2   AAGTTAAAFYVLILISLYITTSASPTGSVCSSTPDPSYCKSALPNQTGNVYSYGRSSFRK 61

Query: 63  SLSSSQKFLRLVEKYLRSRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRAVNSTSRTLPE 122
           SLSSSQKFLRLVEK+LRSRSSLTV AVRALEDCQLLAGLNIDYL TSF+AVN+TSR L E
Sbjct: 62  SLSSSQKFLRLVEKHLRSRSSLTVAAVRALEDCQLLAGLNIDYLRTSFQAVNATSRALTE 121

Query: 123 MKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSLPLAADTKLYSLSLAFFTKGWV 182
           MKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLS PLA+DTKLYSLSLAFFTKGWV
Sbjct: 122 MKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSQPLASDTKLYSLSLAFFTKGWV 181

Query: 183 PKKKKKPTWKAAGRQGGFRNGRMSLKMSSRTKAIYETATRRNLLQTDD--GGDDQIAVRD 242
           PKKKKKPTWKAAGRQGGFRNGRMSLKMSSRT+AIYETATRRNLLQTDD  G DDQIAVRD
Sbjct: 182 PKKKKKPTWKAAGRQGGFRNGRMSLKMSSRTQAIYETATRRNLLQTDDDGGDDDQIAVRD 241

Query: 243 IVVVSQDGSGNFTTINEAISAAPNNSAPSDGYFLIFVSAGIYEEYVSIAKNKRYLMMIGD 302
           IVVVSQDG+GNFTTINEAI+AAPNNSAP+DGYFLIFVSAG+YEEYVSIAKNKRYLMMIGD
Sbjct: 242 IVVVSQDGTGNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVYEEYVSIAKNKRYLMMIGD 301

Query: 303 GINQTVVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGADLS 362
           GINQT+VTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVA+RNGADLS
Sbjct: 302 GINQTIVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVALRNGADLS 361

Query: 363 TFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQFNA 422
           TFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQFNA
Sbjct: 362 TFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQFNA 421

Query: 423 ITAQGRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQSFMDDL 482
           ITAQGRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQSFMDDL
Sbjct: 422 ITAQGRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQSFMDDL 481

Query: 483 IDPEGWRAWNGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGNFVLA 542
           I+P GWRAW+GDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGNFVLA
Sbjct: 482 INPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGNFVLA 541

Query: 543 DDWLPQTGVPYTSGLTD 558
           DDWLPQTGVPYTSGLT+
Sbjct: 542 DDWLPQTGVPYTSGLTE 558

BLAST of CaUC09G163230 vs. ExPASy TrEMBL
Match: A0A1S3BSI2 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103492791 PE=3 SV=1)

HSP 1 Score: 1051.6 bits (2718), Expect = 1.2e-303
Identity = 527/557 (94.61%), Postives = 543/557 (97.49%), Query Frame = 0

Query: 3   ARTTTAAAFYFLILISLYISTSAAPTGSVCSSTRDPSYCKSVLPNQTGNVYSYGRSSFRK 62
           A  TTAAAFY LILISLYI+TSA+PTGSVCSST DPSYCKS LPNQTGNVYSYGRSSFRK
Sbjct: 2   AAGTTAAAFYVLILISLYITTSASPTGSVCSSTPDPSYCKSALPNQTGNVYSYGRSSFRK 61

Query: 63  SLSSSQKFLRLVEKYLRSRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRAVNSTSRTLPE 122
           SLSSSQKFLRLVEK+LRSRSSLTV AVRALEDCQLLAGLNIDYL TSF+AVN+TSR L E
Sbjct: 62  SLSSSQKFLRLVEKHLRSRSSLTVAAVRALEDCQLLAGLNIDYLRTSFQAVNATSRALTE 121

Query: 123 MKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSLPLAADTKLYSLSLAFFTKGWV 182
           MKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLS PLA+DTKLYSLSLAFFTKGWV
Sbjct: 122 MKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSQPLASDTKLYSLSLAFFTKGWV 181

Query: 183 PKKKKKPTWKAAGRQGGFRNGRMSLKMSSRTKAIYETATRRNLLQTDD--GGDDQIAVRD 242
           PKKKKKPTWKAAGRQGGFRNGRMSLKMSSRT+AIYETATRRNLLQTDD  G DDQIAVRD
Sbjct: 182 PKKKKKPTWKAAGRQGGFRNGRMSLKMSSRTQAIYETATRRNLLQTDDDGGDDDQIAVRD 241

Query: 243 IVVVSQDGSGNFTTINEAISAAPNNSAPSDGYFLIFVSAGIYEEYVSIAKNKRYLMMIGD 302
           IVVVSQDG+GNFTTINEAI+AAPNNSAP+DGYFLIFVSAG+YEEYVSIAKNKRYLMMIGD
Sbjct: 242 IVVVSQDGTGNFTTINEAIAAAPNNSAPTDGYFLIFVSAGVYEEYVSIAKNKRYLMMIGD 301

Query: 303 GINQTVVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGADLS 362
           GINQT+VTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVA+RNGADLS
Sbjct: 302 GINQTIVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVALRNGADLS 361

Query: 363 TFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQFNA 422
           TFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQFNA
Sbjct: 362 TFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQFNA 421

Query: 423 ITAQGRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQSFMDDL 482
           ITAQGRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQSFMDDL
Sbjct: 422 ITAQGRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQSFMDDL 481

Query: 483 IDPEGWRAWNGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGNFVLA 542
           I+P GWRAW+GDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGNFVLA
Sbjct: 482 INPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGNFVLA 541

Query: 543 DDWLPQTGVPYTSGLTD 558
           DDWLPQTGVPYTSGLT+
Sbjct: 542 DDWLPQTGVPYTSGLTE 558

BLAST of CaUC09G163230 vs. ExPASy TrEMBL
Match: A0A0A0KB12 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_7G405820 PE=3 SV=1)

HSP 1 Score: 1030.8 bits (2664), Expect = 2.1e-297
Identity = 522/560 (93.21%), Postives = 538/560 (96.07%), Query Frame = 0

Query: 1   MAARTT-TAAAFY-FLILISLYISTSAAPTGSVCSSTRDPSYCKSVLPNQTGNVYSYGRS 60
           MAA TT TAAAFY  LILISLYI+TSA+PTGSVCSST DPSYCKS LPNQTGNVYSYGRS
Sbjct: 1   MAAGTTATAAAFYVLLILISLYITTSASPTGSVCSSTPDPSYCKSALPNQTGNVYSYGRS 60

Query: 61  SFRKSLSSSQKFLRLVEKYLRSRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRAVNSTSR 120
           SFRKSLSSSQKFLRLVEK+LRSRSSLTVPAVRALEDC LLAGLNIDYL TSF+ VN+TSR
Sbjct: 61  SFRKSLSSSQKFLRLVEKHLRSRSSLTVPAVRALEDCLLLAGLNIDYLKTSFQTVNTTSR 120

Query: 121 TLPEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSLPLAADTKLYSLSLAFFT 180
            L EMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLS PLA+DTKLYSLSLAFFT
Sbjct: 121 VLTEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSQPLASDTKLYSLSLAFFT 180

Query: 181 KGWVPKKKKKPTWKAAGRQGGFRNGRMSLKMSSRTKAIYETATRRNLLQTDDGG-DDQIA 240
           KGWVPKKKK+PTWKAAGRQGGFRNGRMSLKMSSRT+AIYE ATRRNLLQTDDGG DDQI 
Sbjct: 181 KGWVPKKKKRPTWKAAGRQGGFRNGRMSLKMSSRTQAIYEKATRRNLLQTDDGGDDDQIK 240

Query: 241 VRDIVVVSQDGSGNFTTINEAISAAPNNSAPSDGYFLIFVSAGIYEEYVSIAKNKRYLMM 300
           VRDIVVVSQDGSGNFTTINEAI+AA NNSAP+DGYFLIFVSAG+YEEYV +AKNKRYLMM
Sbjct: 241 VRDIVVVSQDGSGNFTTINEAIAAATNNSAPTDGYFLIFVSAGVYEEYVLVAKNKRYLMM 300

Query: 301 IGDGINQTVVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGA 360
           IGDGINQT+VTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGA
Sbjct: 301 IGDGINQTIVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGA 360

Query: 361 DLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQ 420
           DLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQ
Sbjct: 361 DLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQ 420

Query: 421 FNAITAQGRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQSFM 480
           FNAITAQGRTDPNQNTGTSIYNCRITAADDLANNS AGVKTFLGRPWKEYSRTVYMQSFM
Sbjct: 421 FNAITAQGRTDPNQNTGTSIYNCRITAADDLANNSDAGVKTFLGRPWKEYSRTVYMQSFM 480

Query: 481 DDLIDPEGWRAWNGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGNF 540
           DDLI+P GWRAW+GDFALNTSYYAEFGNFGPGSNTSERV W GFHLINDTDAGNFTAGNF
Sbjct: 481 DDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVTWAGFHLINDTDAGNFTAGNF 540

Query: 541 VLADDWLPQTGVPYTSGLTD 558
           VLADDWLPQTGVPY SGLT+
Sbjct: 541 VLADDWLPQTGVPYDSGLTE 560

BLAST of CaUC09G163230 vs. ExPASy TrEMBL
Match: A0A6J1GQE6 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111456155 PE=3 SV=1)

HSP 1 Score: 1022.3 bits (2642), Expect = 7.5e-295
Identity = 512/562 (91.10%), Postives = 533/562 (94.84%), Query Frame = 0

Query: 1   MAARTTTAAAFYFLILISLY-----ISTSAAPTGSVCSSTRDPSYCKSVLPNQTGNVYSY 60
           MAA   T AAFY L+LISLY     ISTSAAPTGSVCSS  DPSYCKS LPNQTGNVYSY
Sbjct: 1   MAAAPPTTAAFYSLLLISLYITAAGISTSAAPTGSVCSSAPDPSYCKSALPNQTGNVYSY 60

Query: 61  GRSSFRKSLSSSQKFLRLVEKYLRSRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRAVNS 120
           GRSS RKSLSSSQKFLRLVEKYLRSRSSLTV AVRALEDCQLLAGLNIDYLTTSFR VNS
Sbjct: 61  GRSSVRKSLSSSQKFLRLVEKYLRSRSSLTVAAVRALEDCQLLAGLNIDYLTTSFRTVNS 120

Query: 121 TSRTLPEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSLPLAADTKLYSLSLA 180
           TS TLPEMKA+DVQSLLSAILTNQQTCLDG+KATAGSWSLKNGLSLPLAADTKLYS+SLA
Sbjct: 121 TSWTLPEMKAEDVQSLLSAILTNQQTCLDGLKATAGSWSLKNGLSLPLAADTKLYSMSLA 180

Query: 181 FFTKGWVPKKKKKPTWKAAGRQGGFRNGRMSLKMSSRTKAIYETATRRNLLQTDDGGDDQ 240
           FFTKGWVPKKKKKPTW +AGRQGGF NGRMSLKMSSRTKAIYETATRRNLLQT+DGGDDQ
Sbjct: 181 FFTKGWVPKKKKKPTWTSAGRQGGFNNGRMSLKMSSRTKAIYETATRRNLLQTEDGGDDQ 240

Query: 241 IAVRDIVVVSQDGSGNFTTINEAISAAPNNSAPSDGYFLIFVSAGIYEEYVSIAKNKRYL 300
           I+VRDIVVVSQDGSGNFTTINEAI+AAPNNSAP+DGYFLIFV AG+YEEYV+IAKNKRYL
Sbjct: 241 ISVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVDAGVYEEYVTIAKNKRYL 300

Query: 301 MMIGDGINQTVVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRN 360
           MMIGDGINQTVVTGNR+VVDGWTTFNSATFAVVGPGFVAVNMTFRN AGAIKHQAVAVRN
Sbjct: 301 MMIGDGINQTVVTGNRNVVDGWTTFNSATFAVVGPGFVAVNMTFRNAAGAIKHQAVAVRN 360

Query: 361 GADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMS 420
            ADLSTFYLCSFE YQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVV QNCNIYPRLPM+
Sbjct: 361 SADLSTFYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVLQNCNIYPRLPMA 420

Query: 421 NQFNAITAQGRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQS 480
           NQFNAITAQGRTDPNQNTGTSI+NCRITAADDLANNSGA V TFLGRPWKEYSRTVYMQS
Sbjct: 421 NQFNAITAQGRTDPNQNTGTSIHNCRITAADDLANNSGARVNTFLGRPWKEYSRTVYMQS 480

Query: 481 FMDDLIDPEGWRAWNGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAG 540
           FMDDLI+P GWRAW+GDFALNT YYAEFGNFGPGSNTSERVNW GFHLI+DTDAGNFTAG
Sbjct: 481 FMDDLINPAGWRAWDGDFALNTLYYAEFGNFGPGSNTSERVNWVGFHLIDDTDAGNFTAG 540

Query: 541 NFVLADDWLPQTGVPYTSGLTD 558
           NF++ADDW+PQTGVPYTSGLT+
Sbjct: 541 NFLMADDWVPQTGVPYTSGLTE 562

BLAST of CaUC09G163230 vs. ExPASy TrEMBL
Match: A0A6J1JVJ5 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111487941 PE=3 SV=1)

HSP 1 Score: 1014.2 bits (2621), Expect = 2.1e-292
Identity = 507/562 (90.21%), Postives = 531/562 (94.48%), Query Frame = 0

Query: 1   MAARTTTAAAFYFLILISLY-----ISTSAAPTGSVCSSTRDPSYCKSVLPNQTGNVYSY 60
           MAA   T  AFY+L+LISLY     ISTSAAPTGSVCSS  DPSYCKS LPNQTGNVYSY
Sbjct: 1   MAAAPPTTPAFYYLLLISLYITAAGISTSAAPTGSVCSSAPDPSYCKSALPNQTGNVYSY 60

Query: 61  GRSSFRKSLSSSQKFLRLVEKYLRSRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRAVNS 120
           GRSS RKSLSSSQKFLRLVEKYLRSRSSLTV AVR+LEDCQLLAGLNIDYLTTSFR VNS
Sbjct: 61  GRSSVRKSLSSSQKFLRLVEKYLRSRSSLTVAAVRSLEDCQLLAGLNIDYLTTSFRTVNS 120

Query: 121 TSRTLPEMKADDVQSLLSAILTNQQTCLDGIKATAGSWSLKNGLSLPLAADTKLYSLSLA 180
           TS TLPEMKA+DVQSLLSAILTNQQTCLDG+KATAGSWSLKNGLSLPL ADTKLYS+SLA
Sbjct: 121 TSWTLPEMKAEDVQSLLSAILTNQQTCLDGLKATAGSWSLKNGLSLPLDADTKLYSMSLA 180

Query: 181 FFTKGWVPKKKKKPTWKAAGRQGGFRNGRMSLKMSSRTKAIYETATRRNLLQTDDGGDDQ 240
           FFTKGWVPKKKKK TW +AGRQGGF NGRMS KMSSRTKAIYE ATRRNLLQTDDGGDDQ
Sbjct: 181 FFTKGWVPKKKKKQTWTSAGRQGGFSNGRMSFKMSSRTKAIYEMATRRNLLQTDDGGDDQ 240

Query: 241 IAVRDIVVVSQDGSGNFTTINEAISAAPNNSAPSDGYFLIFVSAGIYEEYVSIAKNKRYL 300
           I+VRDIVVVSQDGSGNFTTINEAI+AAPNNSAP+DGYFLIFV AG+YEEYV+IAKNKRYL
Sbjct: 241 ISVRDIVVVSQDGSGNFTTINEAIAAAPNNSAPTDGYFLIFVDAGVYEEYVTIAKNKRYL 300

Query: 301 MMIGDGINQTVVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRN 360
           MMIGDGINQTVVTGNR+VVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRN
Sbjct: 301 MMIGDGINQTVVTGNRNVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRN 360

Query: 361 GADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMS 420
            ADLSTFYLCSFE YQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVV QNCNIYPRLPM+
Sbjct: 361 SADLSTFYLCSFEGYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVLQNCNIYPRLPMA 420

Query: 421 NQFNAITAQGRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQS 480
           NQFNAITAQGRTDPNQNTGTSI+NCRITAADDLANN+GA V TFLGRPWKEYSRTVYMQS
Sbjct: 421 NQFNAITAQGRTDPNQNTGTSIHNCRITAADDLANNTGARVNTFLGRPWKEYSRTVYMQS 480

Query: 481 FMDDLIDPEGWRAWNGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAG 540
           FM+DLI+P GWRAW+GDFALNTSYYAEFGNFGPGSNTSERVNW GFHLI+DTDAGNFTAG
Sbjct: 481 FMNDLINPAGWRAWDGDFALNTSYYAEFGNFGPGSNTSERVNWVGFHLIDDTDAGNFTAG 540

Query: 541 NFVLADDWLPQTGVPYTSGLTD 558
           NF++ADDW+PQTGVPYTSGLT+
Sbjct: 541 NFLMADDWVPQTGVPYTSGLTE 562

BLAST of CaUC09G163230 vs. TAIR 10
Match: AT1G02810.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 706.8 bits (1823), Expect = 1.4e-203
Identity = 357/573 (62.30%), Postives = 433/573 (75.57%), Query Frame = 0

Query: 12  YFLILISLYI------STSAAPTGSVCSSTRDPSYCKSVLPNQTGNVYSYGRSSFRKSLS 71
           + LI +S ++      S + + + ++C +T DP YCKSV P+  GNV  YG  S RKSLS
Sbjct: 6   FILITLSFFLQSVLASSQTLSNSSTICKTTPDPKYCKSVFPHSQGNVQQYGCFSIRKSLS 65

Query: 72  SSQKFLRLVEKYLRSRSSLTVPAV-RALEDCQLLAGLNIDYLTTSFRAVNSTS-----RT 131
            S+KF+R V++Y++  + L+ PAV RAL+DC+ LAGL +DYL TSF  VN TS     + 
Sbjct: 66  QSRKFIRTVDRYIKRNAHLSQPAVIRALQDCRFLAGLTMDYLLTSFETVNDTSAKTSFKP 125

Query: 132 LPEMKADDVQSLLSAILTNQQTCLDGIKATA---GSWSLKNGLSLPLAADTKLYSLSLAF 191
           L   KADD+Q+LLSA LTN+QTCL+G+   A    +W+++ G++LPL  DTKL  +SLA 
Sbjct: 126 LSFPKADDIQTLLSAALTNEQTCLEGLTTAASYSATWTVRTGVALPLVNDTKLLGVSLAL 185

Query: 192 FTKGWVPKKKKKPTWKAAGRQGG----------FRNGRMSLKMSSRTKAIYETATRRNLL 251
           FTKGWVPKKKK+  +  A  + G          FRNG + LKM+ +TKA+YE+ +RR L 
Sbjct: 186 FTKGWVPKKKKRAGFAWAQPRSGSSTHTKPFRLFRNGALPLKMTEKTKAVYESLSRRKLA 245

Query: 252 QTDDGGD----DQIAVRDIVVVSQDGSGNFTTINEAISAAPNNSAPSDGYFLIFVSAGIY 311
             D  GD      + + DIV VSQDG+GNFT I  A++AAPNN+  S G+FLI+V+AGIY
Sbjct: 246 DGDSNGDGDDGSMVLISDIVTVSQDGTGNFTNITAAVAAAPNNTDGSAGFFLIYVTAGIY 305

Query: 312 EEYVSIAKNKRYLMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNT 371
           EEY+SIAKNKRY+MMIGDGINQTVVTGNRSVVDGWTTFNSATFAV  P FVAVN+TFRNT
Sbjct: 306 EEYISIAKNKRYMMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVTAPNFVAVNITFRNT 365

Query: 372 AGAIKHQAVAVRNGADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVV 431
           AG  KHQAVA+R+GAD S FY CSFEAYQDTLYTHSLRQFYR+CD+YGTVDFIFGNAAVV
Sbjct: 366 AGPEKHQAVALRSGADFSIFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVV 425

Query: 432 FQNCNIYPRLPMSNQFNAITAQGRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGR 491
           FQNCN+YPR PM NQFNAITAQGR+DPNQNTGTSI NC I  ADDL  +S   VKT+LGR
Sbjct: 426 FQNCNLYPRKPMPNQFNAITAQGRSDPNQNTGTSIQNCTIKPADDLV-SSNYTVKTYLGR 485

Query: 492 PWKEYSRTVYMQSFMDDLIDPEGWRAWNGDFALNTSYYAEFGNFGPGSNTSERVNWPGFH 551
           PWKEYSRTVYMQS++D  ++P GWR WNGDFAL+T YYAE+ N GPGSNT+ RV WPG+H
Sbjct: 486 PWKEYSRTVYMQSYIDGFVEPVGWREWNGDFALSTLYYAEYNNTGPGSNTTNRVTWPGYH 545

Query: 552 LINDTDAGNFTAGNFVLADDWLPQTGVPYTSGL 556
           +IN TDA NFT     +  DW+ +TGVPYTSGL
Sbjct: 546 VINSTDAANFTVTGLFIEADWIWKTGVPYTSGL 577

BLAST of CaUC09G163230 vs. TAIR 10
Match: AT4G02330.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 684.9 bits (1766), Expect = 5.5e-197
Identity = 354/562 (62.99%), Postives = 434/562 (77.22%), Query Frame = 0

Query: 13  FLILISLYI-STSAAPTGS---VCSSTRDPSYCKSVLPNQT-GNVYSYGRSSFRKSLSSS 72
           FL L +L+I S +  P+ S   +C +T DP +CKSV P  + G+V  YGR S RKSL+ S
Sbjct: 12  FLSLQTLFIASQTLLPSNSSSTICKTTPDPKFCKSVFPQTSQGDVREYGRFSLRKSLTQS 71

Query: 73  QKFLRLVEKYL-RSRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRAVNST--SRTLPEMK 132
           +KF R +++YL R+ + L+  AV AL+DC+ LA L  DYL TSF  VN T  S+TL   K
Sbjct: 72  RKFTRTIDRYLKRNNALLSQSAVGALQDCRYLASLTTDYLITSFETVNITTSSKTLSFSK 131

Query: 133 ADDVQSLLSAILTNQQTCLDGIK-ATAGSWSLKNGLSLPLAADTKLYSLSLAFFTKGWVP 192
           AD++Q+LLSA LTN+QTCLDGI  A + SW+++NG++LPL  DTKL+S+SLA FTKGWVP
Sbjct: 132 ADEIQTLLSAALTNEQTCLDGINTAASSSWTIRNGVALPLINDTKLFSVSLALFTKGWVP 191

Query: 193 KKKK----------KPTWKAAGRQGGFRNGRMSLKMSSRTKAIYETATRRNLLQTDDGGD 252
           KKKK          K T         FRNG + LKM+  T+A+YE+ +RR L   DD   
Sbjct: 192 KKKKQVASYSWAHPKNTHSHTKPFRHFRNGALPLKMTEHTRAVYESLSRRKLAD-DDNDV 251

Query: 253 DQIAVRDIVVVSQDGSGNFTTINEAISAAPNNSAPSDGYFLIFVSAGIYEEYVSIAKNKR 312
           + + V DIV V+Q+G+GNFTTI EA+++APN +  + GYF+I+V++G+YEE V IAKNKR
Sbjct: 252 NTVLVSDIVTVNQNGTGNFTTITEAVNSAPNKTDGTAGYFVIYVTSGVYEENVVIAKNKR 311

Query: 313 YLMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAV 372
           YLMMIGDGIN+TVVTGNR+VVDGWTTFNSATFAV  P FVAVNMTFRNTAG  KHQAVA+
Sbjct: 312 YLMMIGDGINRTVVTGNRNVVDGWTTFNSATFAVTSPNFVAVNMTFRNTAGPEKHQAVAM 371

Query: 373 RNGADLSTFYLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLP 432
           R+ ADLS FY CSFEAYQDTLYTHSLRQFYR+CDIYGTVDFIFGNAAVVFQ+CN+YPR P
Sbjct: 372 RSSADLSIFYSCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQDCNLYPRQP 431

Query: 433 MSNQFNAITAQGRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYM 492
           M NQFNAITAQGRTDPNQNTG SI+NC I  ADDL  +S   VKT+LGRPWKEYSRTV+M
Sbjct: 432 MQNQFNAITAQGRTDPNQNTGISIHNCTIKPADDLV-SSNYTVKTYLGRPWKEYSRTVFM 491

Query: 493 QSFMDDLIDPEGWRAWNGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFT 552
           QS++D++++P GWR WNGDFAL+T YYAE+ N G GS+T++RV WPG+H+IN TDA NFT
Sbjct: 492 QSYIDEVVEPVGWREWNGDFALSTLYYAEYNNTGSGSSTTDRVVWPGYHVINSTDANNFT 551

Query: 553 AGNFVLADDWLPQTGVPYTSGL 556
             NF+L D W+ Q+GVPY SGL
Sbjct: 552 VENFLLGDGWMVQSGVPYISGL 571

BLAST of CaUC09G163230 vs. TAIR 10
Match: AT2G47550.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 657.5 bits (1695), Expect = 9.4e-189
Identity = 324/537 (60.34%), Postives = 411/537 (76.54%), Query Frame = 0

Query: 24  SAAPTGSVCSSTRDPSYCKSVLPNQTGNVYSYGRSSFRKSLSSSQKFLRLVEKYLRSRSS 83
           +A    ++C    DPSYC+SVLPNQ G++YSYGR S R+SLS +++F+ +++  L  +  
Sbjct: 31  NATSPSNICRFAPDPSYCRSVLPNQPGDIYSYGRLSLRRSLSRARRFISMIDAELDRKGK 90

Query: 84  LTVPA-VRALEDCQLLAGLNIDYLTTSFRAVNSTSRTLPEMKADDVQSLLSAILTNQQTC 143
           +   + V ALEDC+ LA L +DYL +S +  +ST +TL   +A+DV + LSA +TN+QTC
Sbjct: 91  VAAKSTVGALEDCKFLASLTMDYLLSSSQTADST-KTLSLSRAEDVHTFLSAAITNEQTC 150

Query: 144 LDGIKATAGSWSLKNGLSLPLAADTKLYSLSLAFFTKGWVPKKKK-KPTWKAAGRQG--- 203
           L+G+K+TA     +NGLS  L  DTKLY +SLA F+KGWVP++++ +P W+   R     
Sbjct: 151 LEGLKSTAS----ENGLSGDLFNDTKLYGVSLALFSKGWVPRRQRSRPIWQPQARFKKFF 210

Query: 204 GFRNGRMSLKMSSRTKAIYETATRRNLLQTDDGGDDQIAVRDIVVVSQDGSGNFTTINEA 263
           GFRNG++ LKM+ R +A+Y T TRR LLQ+D    D + V DIV V Q+G+GNFTTIN A
Sbjct: 211 GFRNGKLPLKMTERARAVYNTVTRRKLLQSD---ADAVQVSDIVTVIQNGTGNFTTINAA 270

Query: 264 ISAAPNNSAPSDGYFLIFVSAGIYEEYVSIAKNKRYLMMIGDGINQTVVTGNRSVVDGWT 323
           I+AAPN +  S+GYFLI+V+AG+YEEYV + KNKRY+MMIGDGINQTV+TGNRSVVDGWT
Sbjct: 271 IAAAPNKTDGSNGYFLIYVTAGLYEEYVEVPKNKRYVMMIGDGINQTVITGNRSVVDGWT 330

Query: 324 TFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGADLSTFYLCSFEAYQDTLYTHS 383
           TFNSATF + GP F+ VN+T RNTAG  K QAVA+R+G DLS FY CSFEAYQDTLYTHS
Sbjct: 331 TFNSATFILSGPNFIGVNITIRNTAGPTKGQAVALRSGGDLSVFYSCSFEAYQDTLYTHS 390

Query: 384 LRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQFNAITAQGRTDPNQNTGTSIY 443
           LRQFYR+CD+YGTVDFIFGNAAVV QNCN+YPR P   Q N +TAQGRTDPNQNTGT+I+
Sbjct: 391 LRQFYRECDVYGTVDFIFGNAAVVLQNCNLYPRQPRKGQSNEVTAQGRTDPNQNTGTAIH 450

Query: 444 NCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQSFMDDLIDPEGWRAWNGDFALNTS 503
            C I  ADDLA  S   VKT+LGRPWKEYSRTV MQ+++D  ++P GW AW+GDFAL+T 
Sbjct: 451 GCTIRPADDLA-TSNYTVKTYLGRPWKEYSRTVVMQTYIDGFLEPSGWNAWSGDFALSTL 510

Query: 504 YYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGNFVLADDWLPQTGVPYTSGL 556
           YYAE+ N GPGS+T+ RV WPG+H+IN TDA NFT  NF++ + W+ QTGVP+  GL
Sbjct: 511 YYAEYNNTGPGSDTTNRVTWPGYHVINATDASNFTVTNFLVGEGWIGQTGVPFVGGL 558

BLAST of CaUC09G163230 vs. TAIR 10
Match: AT3G10720.2 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 485.7 bits (1249), Expect = 4.9e-137
Identity = 260/553 (47.02%), Postives = 351/553 (63.47%), Query Frame = 0

Query: 18  SLYISTSAAPTGSVCSSTRDPSYCKSVL---PNQTGNVYSYGRSSFRKSLSSSQKFLRLV 77
           SL  S S +P+   C ST  P  C+++L    +   + Y YG+ + ++ L  + +  +++
Sbjct: 70  SLPPSQSQSPS-LACKSTPYPKLCRTILNAVKSSPSDPYRYGKFTIKQCLKQASRLSKVI 129

Query: 78  EKYLR----SRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRAVNSTSRTLPEMKADDVQS 137
             Y R       S T   + A+ DC  L+ L+++YL T    +  T++ +     + V S
Sbjct: 130 TSYARRVESKPGSATAEEIGAVADCGELSELSVNYLETVTTEL-KTAQVMTAALVEHVNS 189

Query: 138 LLSAILTNQQTCLDGIKATAGSWSLKNGLSLPLAADTKLYSLSLAFFTKGWVPKKKKKPT 197
           LLS ++TNQQTCLDG+      ++    +  P+   T+LYS+SL   +       K+   
Sbjct: 190 LLSGVVTNQQTCLDGLVEAKSGFAA--AIGSPMGNLTRLYSISLGLVSHALNRNLKRFKA 249

Query: 198 WKAAGRQGGFRNGRMSLKMSSRTKAIYETA--------TRRNLLQTDDGGDDQIAVRDIV 257
            K     GG  N      + +  K + +T         T RNL +  +     I V   V
Sbjct: 250 SKGKILGGG--NSTYREPLETLIKGLRKTCDNDKDCRKTSRNLGELGETSGGSILVSKAV 309

Query: 258 VVSQDGSGNFTTINEAISAAPNNSAPSDGYFLIFVSAGIYEEYVSIAKNKRYLMMIGDGI 317
           +V    S NFTTI +AI+AAPNN+ P DGYF+I+   G+YEEY+ +  NK+ LM++GDGI
Sbjct: 310 IVGPFKSDNFTTITDAIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLMLMGDGI 369

Query: 318 NQTVVTGNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGADLSTF 377
           N+T++TGN +VVDGWTT+N ++FAVVG  F+AV++TFRNTAG  KHQAVA+RN A+ S+F
Sbjct: 370 NKTIITGNHNVVDGWTTYNCSSFAVVGERFMAVDVTFRNTAGPEKHQAVALRNNAEGSSF 429

Query: 378 YLCSFEAYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQFNAIT 437
           Y CSFE YQDTLY HSLRQFYR+CDIYGTVDFIFGNAA +FQNCNIY R PM+ Q NAIT
Sbjct: 430 YRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAIFQNCNIYARKPMAKQKNAIT 489

Query: 438 AQGRTDPNQNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQSFMDDLID 497
           A GR DPNQNTG SI NC I AA DLA    + + TFLGRPWK YSRTV+MQS++ D++ 
Sbjct: 490 AHGRLDPNQNTGISIINCTIKAAPDLAAEPKSAM-TFLGRPWKPYSRTVFMQSYISDIVQ 549

Query: 498 PEGWRAWNGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGNFVLADD 556
           P GW  WNG   L+T YY E+ NFGPG+NT++RV W G++L+N  +A NFT  NF + D 
Sbjct: 550 PVGWLEWNGTIGLDTIYYGEYSNFGPGANTNQRVQWLGYNLLNLAEAMNFTVYNFTMGDT 609

BLAST of CaUC09G163230 vs. TAIR 10
Match: AT5G04970.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 484.6 bits (1246), Expect = 1.1e-136
Identity = 259/547 (47.35%), Postives = 346/547 (63.25%), Query Frame = 0

Query: 24  SAAPTGSVCSSTRDPSYCKSVL---PNQTGNVYSYGRSSFRKSLSSSQKFLRLVEKYLR- 83
           S  P    C ST  P  C+++L    +   + Y YG+ + ++ L  +++  +++ ++ + 
Sbjct: 80  SPLPPNIACKSTPYPKLCRTILSAVKSSPSDPYHYGKFTMKQCLKQARRLSKVINRFAQR 139

Query: 84  ---SRSSLTVPAVRALEDCQLLAGLNIDYLTTSFRAVNSTSRTLPEMKADDVQSLLSAIL 143
                 + TV  V A+ DC  LA L+++YL T    + + +  +     D V SLL  ++
Sbjct: 140 VEADPGTSTVEEVSAVADCGELAELSVEYLETVTEELKA-AELMTAALVDRVTSLLGGVV 199

Query: 144 TNQQTCLDGIKATAGSWSLKNGLSLPLAADTKLYSLSLAFFTKGWVPKKKKKPTWKAAGR 203
           TNQQTCLDG+      ++    +  PL   T+LYS+SL   +       K+    K  G+
Sbjct: 200 TNQQTCLDGLVDAKSGFA--TAIGTPLGNLTRLYSVSLGLVSHALNRNLKRYKGSK--GK 259

Query: 204 QGGFRNGRMSLKMSSRTKAIYETATR--------RNLLQTDDGGDDQIAVRDIVVVSQDG 263
             G  N  +   + +  K + +T  +        RNL +  +     I VR+ V V    
Sbjct: 260 IFGGGNKPVREPLETLIKVLRKTCDKGKDCRKANRNLGELGETSGGSILVREAVTVGPYE 319

Query: 264 SGNFTTINEAISAAPNNSAPSDGYFLIFVSAGIYEEYVSIAKNKRYLMMIGDGINQTVVT 323
           + NF TI EA++AAPN++ P  GYF+I+  AG+YEEYV I+  KR +M+IGDGIN+T+++
Sbjct: 320 TDNFPTITEAVAAAPNHTFPEQGYFVIYARAGLYEEYVVISNKKRNIMLIGDGINKTIIS 379

Query: 324 GNRSVVDGWTTFNSATFAVVGPGFVAVNMTFRNTAGAIKHQAVAVRNGADLSTFYLCSFE 383
           GN S +DGWTT+NS+TFAVVG  FVAV++TFRNTAG  KHQAVAVRN AD STFY CSFE
Sbjct: 380 GNHSFIDGWTTYNSSTFAVVGDRFVAVDVTFRNTAGPEKHQAVAVRNNADGSTFYRCSFE 439

Query: 384 AYQDTLYTHSLRQFYRDCDIYGTVDFIFGNAAVVFQNCNIYPRLPMSNQFNAITAQGRTD 443
            YQDTLY HSLRQFYR+CDIYGT+DFIFGNAA +FQNCNIY R PM+NQ NA+TA GRTD
Sbjct: 440 GYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAAIFQNCNIYARKPMANQKNAVTAHGRTD 499

Query: 444 PNQNTGTSIYNCRITAADDLANNSGAGVKTFLGRPWKEYSRTVYMQSFMDDLIDPEGWRA 503
           PNQ TG SI NC I AA DLA +      TFLGRPWK YSRTVY+QS++ D++ P GW  
Sbjct: 500 PNQKTGISIINCTIGAAPDLAADP-KSTMTFLGRPWKPYSRTVYIQSYISDVVQPVGWLE 559

Query: 504 WNGDFALNTSYYAEFGNFGPGSNTSERVNWPGFHLINDTDAGNFTAGNFVLADDWLPQTG 556
           WNG   L+T  Y E+ NFGPG++TS+RV W G+ L+N   A NFT  NF L D WLPQT 
Sbjct: 560 WNGTTGLDTISYGEYDNFGPGADTSKRVQWSGYSLLNLVQAMNFTVYNFTLGDTWLPQTD 619

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038896831.10.0e+0096.26probable pectinesterase/pectinesterase inhibitor 7 [Benincasa hispida][more]
XP_008451544.12.4e-30394.61PREDICTED: probable pectinesterase/pectinesterase inhibitor 7 [Cucumis melo] >KA... [more]
XP_004136013.14.4e-29793.21probable pectinesterase/pectinesterase inhibitor 7 [Cucumis sativus] >KGN44976.1... [more]
KAG7014346.14.1e-29591.28putative pectinesterase/pectinesterase inhibitor 41, partial [Cucurbita argyrosp... [more]
KAG6575804.15.4e-29591.28putative pectinesterase/pectinesterase inhibitor 41, partial [Cucurbita argyrosp... [more]
Match NameE-valueIdentityDescription
Q9SRX41.9e-20262.30Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=37... [more]
Q8RXK77.7e-19662.99Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana OX=3... [more]
O222561.3e-18760.34Probable pectinesterase/pectinesterase inhibitor 20 OS=Arabidopsis thaliana OX=3... [more]
Q94CB16.9e-13647.02Probable pectinesterase/pectinesterase inhibitor 25 OS=Arabidopsis thaliana OX=3... [more]
Q9FF771.5e-13547.35Probable pectinesterase/pectinesterase inhibitor 47 OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
A0A5D3D3H41.2e-30394.61Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G00132... [more]
A0A1S3BSI21.2e-30394.61Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103492791 PE=3 SV=1[more]
A0A0A0KB122.1e-29793.21Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_7G405820 PE=3 SV=1[more]
A0A6J1GQE67.5e-29591.10Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111456155 PE=3 SV=1[more]
A0A6J1JVJ52.1e-29290.21Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111487941 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G02810.11.4e-20362.30Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT4G02330.15.5e-19762.99Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT2G47550.19.4e-18960.34Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT3G10720.24.9e-13747.02Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT5G04970.11.1e-13647.35Plant invertase/pectin methylesterase inhibitor superfamily [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (USVL246-FR2) v1
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 22..176
e-value: 3.0E-22
score: 89.9
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 30..175
e-value: 1.1E-16
score: 61.5
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 242..539
e-value: 2.9E-135
score: 449.8
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 28..179
e-value: 2.5E-20
score: 75.0
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 30..179
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 236..555
e-value: 1.4E-118
score: 398.0
NoneNo IPR availablePANTHERPTHR31707:SF294PECTINESTERASE/PECTINESTERASE INHIBITOR 7-RELATEDcoord: 13..555
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 13..555
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 30..178
e-value: 1.14508E-27
score: 106.754
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 388..397
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 237..554

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CaUC09G163230.1CaUC09G163230.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity
molecular_function GO:0004857 enzyme inhibitor activity