CaUC09G162250 (gene) Watermelon (USVL246-FR2) v1

Overview
NameCaUC09G162250
Typegene
OrganismCitrullus amarus (Watermelon (USVL246-FR2) v1)
DescriptionDelta(24)-sterol reductase
LocationCiama_Chr09: 4252592 .. 4254908 (-)
RNA-Seq ExpressionCaUC09G162250
SyntenyCaUC09G162250
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCAGATTTAGAGGTTCCTCTGCGCCCAAAGAGGAAGAAGGTCTGGGTGGACTATTTTGTGCAATTCAGATGGATTTTAGTTATTTTTGTTGTTCTTCCTATATCATTCAGCCTATACTTCTTCACATATCTTGGGGACGTCAGATCTGCCTGGAAGTCCTACAAGAAACGGCAAAAGGAACATGATGAAAATGTCAAGAAAGTTGTAAAGCGCCTTAAACAAAGGAACCCAGCAAAGGATGGTCTTGTTTGCACAGCTCGCAAGCCTTGGATTGCTGTTGGAATGCGAAATGTTGACTACAAACGAGCACGTCACTTTGAGGTTGATTTATCAGCTTTTCGAAATATTTTGGATATTGACAAAGATAGAATGATTGCCAAAGTTGAACCCCTTGTGAACATGGGACAAATAAGCAGGGCCACTGTTCCACTCAATCTTTCTCTTGCTGTAGTTGCTGAACTTGACGATCTTACTGTTGGAGGACTCATCAATGGCTATGGAATTGAAGGAAGCTCCCATCTCTATGGCTTGTTCTCGGACACTGTTGTGGCATATGAGATTGTTCTAGCAGATGGCCAGGTTGTTAGAGCTACTAAAGATAATGAGTACTCAGATCTTTTCTATGCAATCCCATGGTCCCAAGGAACAATTGGTCTTCTTGTTGCTGCTGAGATTAAGCTCATACCCATTAAAGAGTATATGAAAGTCACATACAAACCCTTTAGAGGGACATTGAGAGAGATTGGACAAGCTTATATGGATTCTCTTGCCCCTAGAGATGGAGATCAAGATAACCCAGAAAAGGTTCCCGACTTTGTTGAAACTATGATTTATACCCCTTCTGAAGCTGTGGTCATGACAGGTAGATATGCTTCCAAAGAAGAGGCTAAAAAAAGGGGAAATGTGATAAATCAGATTGGTTGGTGGTTCAAACCATGGTTCTACCAGCATGCATCAACAGCACTCAAGAAAGGGGAGTTTGTTGAGTATATCCCTACAAGGGATTACTATCATAGGCACACCAGATCTTTGTATTGGGAGGGAAAGCTTATCCTTCCCTTTGCAGATCAATGGTGGTTCAGATTTTTATTGGGATGGATGATGCCTCCCAAAGTTTCTCTGCTCAAAGCCACTCAAGGTGAAGCTATCAGAAATTATTATCATGAAATGCATGTTATTCAAGACATGCTTGTTCCTCTTTACAAGGTTCCGGATGCTTTGGAGTGGGCTCATCGTGAGTTTGAGGTATTGCTTGTCCTCCCTGACACCTTTGTTCGATAAATTACAAGTTTAGTTTCGAAATCTTATGTTTGTGTCTAGTAAGCCTCTAAATTTTAATTTGTCTAGTATAGTCCTAAAATTTTAATTTTAAAATGCACAAACCTATTAGATGCAAAATTGTAACTTTTAACTTATTGTTAGACACAAAAATGATAAGTTTAGCTATTAGAACAAAATTCAATTTTATATTTAATAGATTCATTAACTTAAGAATTTGGAATTTGTCAATGATCTATTTTATGCAAAATAAAAAATTAAGAAATTTGTTGTACACAAAGCTTAAAGTTTAGAGTTTCGATAGACACATTTTAAACAACATTGTTAAATCATTGATTGACTCAAAAATTTAAACTTATGGGTGAACATAAATTTAATATAATATCGTTAACAAACATGACCCTACTAAACACAATCACTGAACTTTCGAGACTAAGCTTGTACTAGCTAACTTATATATTTGATAGAGGTGGTTATAGGCTAATGATTGATGAGTCTTCTTATCAAAGGTGGATTTCAATTTTCAATTGAATTATGCAGTCCATTTGGTTAACAAGAACAATTTGTTTGTGTTCTTGCACAGGTTTACCCTATTTGGCTCTGTCCACACAGAATCTTCAAGCTTCCAGTCAAAACCATGATATATCCCGAACCAGGATTCGAGTTACATCGTCGACAAGGCGACACACATTACGCTCAAATGTACACAGATGTTGGACTGTACTATGCACCAGGCCCTGTCCTGAGGGGCGAAGTTTTCGATGGTGCCGAGGCTGTTCGTAGAATGGAGAAATGGCTTCTAGAAAACCATGGATTCCAGGCTCTATATGCAGTATCAGAACTGAATGAGAAAGATTTCTGGAGGATGTTTGATGCTGAACTCTATGAACAATGCCGGAAGAAATATGGAGCTGTAGGAACCTTTATGACAGTCTATTACAAGTCCAAGAAAGGTCGAAAAACCGAGAAGGAAGTGAGGGAAGCTGAACAAGCTCATCTTGAAACTACTTATGCTGAATTGGAACAACCCAATTAA

mRNA sequence

ATGTCAGATTTAGAGGTTCCTCTGCGCCCAAAGAGGAAGAAGGTCTGGGTGGACTATTTTGTGCAATTCAGATGGATTTTAGTTATTTTTGTTGTTCTTCCTATATCATTCAGCCTATACTTCTTCACATATCTTGGGGACGTCAGATCTGCCTGGAAGTCCTACAAGAAACGGCAAAAGGAACATGATGAAAATGTCAAGAAAGTTGTAAAGCGCCTTAAACAAAGGAACCCAGCAAAGGATGGTCTTGTTTGCACAGCTCGCAAGCCTTGGATTGCTGTTGGAATGCGAAATGTTGACTACAAACGAGCACGTCACTTTGAGGTTGATTTATCAGCTTTTCGAAATATTTTGGATATTGACAAAGATAGAATGATTGCCAAAGTTGAACCCCTTGTGAACATGGGACAAATAAGCAGGGCCACTGTTCCACTCAATCTTTCTCTTGCTGTAGTTGCTGAACTTGACGATCTTACTGTTGGAGGACTCATCAATGGCTATGGAATTGAAGGAAGCTCCCATCTCTATGGCTTGTTCTCGGACACTGTTGTGGCATATGAGATTGTTCTAGCAGATGGCCAGGTTGTTAGAGCTACTAAAGATAATGAGTACTCAGATCTTTTCTATGCAATCCCATGGTCCCAAGGAACAATTGGTCTTCTTGTTGCTGCTGAGATTAAGCTCATACCCATTAAAGAGTATATGAAAGTCACATACAAACCCTTTAGAGGGACATTGAGAGAGATTGGACAAGCTTATATGGATTCTCTTGCCCCTAGAGATGGAGATCAAGATAACCCAGAAAAGGTTCCCGACTTTGTTGAAACTATGATTTATACCCCTTCTGAAGCTGTGGTCATGACAGGTAGATATGCTTCCAAAGAAGAGGCTAAAAAAAGGGGAAATGTGATAAATCAGATTGGTTGGTGGTTCAAACCATGGTTCTACCAGCATGCATCAACAGCACTCAAGAAAGGGGAGTTTGTTGAGTATATCCCTACAAGGGATTACTATCATAGGCACACCAGATCTTTGTATTGGGAGGGAAAGCTTATCCTTCCCTTTGCAGATCAATGGTGGTTCAGATTTTTATTGGGATGGATGATGCCTCCCAAAGTTTCTCTGCTCAAAGCCACTCAAGGTGAAGCTATCAGAAATTATTATCATGAAATGCATGTTATTCAAGACATGCTTGTTCCTCTTTACAAGGTTCCGGATGCTTTGGAGTGGGCTCATCGTGAGTTTGAGGTTTACCCTATTTGGCTCTGTCCACACAGAATCTTCAAGCTTCCAGTCAAAACCATGATATATCCCGAACCAGGATTCGAGTTACATCGTCGACAAGGCGACACACATTACGCTCAAATGTACACAGATGTTGGACTGTACTATGCACCAGGCCCTGTCCTGAGGGGCGAAGTTTTCGATGGTGCCGAGGCTGTTCGTAGAATGGAGAAATGGCTTCTAGAAAACCATGGATTCCAGGCTCTATATGCAGTATCAGAACTGAATGAGAAAGATTTCTGGAGGATGTTTGATGCTGAACTCTATGAACAATGCCGGAAGAAATATGGAGCTGTAGGAACCTTTATGACAGTCTATTACAAGTCCAAGAAAGGTCGAAAAACCGAGAAGGAAGTGAGGGAAGCTGAACAAGCTCATCTTGAAACTACTTATGCTGAATTGGAACAACCCAATTAA

Coding sequence (CDS)

ATGTCAGATTTAGAGGTTCCTCTGCGCCCAAAGAGGAAGAAGGTCTGGGTGGACTATTTTGTGCAATTCAGATGGATTTTAGTTATTTTTGTTGTTCTTCCTATATCATTCAGCCTATACTTCTTCACATATCTTGGGGACGTCAGATCTGCCTGGAAGTCCTACAAGAAACGGCAAAAGGAACATGATGAAAATGTCAAGAAAGTTGTAAAGCGCCTTAAACAAAGGAACCCAGCAAAGGATGGTCTTGTTTGCACAGCTCGCAAGCCTTGGATTGCTGTTGGAATGCGAAATGTTGACTACAAACGAGCACGTCACTTTGAGGTTGATTTATCAGCTTTTCGAAATATTTTGGATATTGACAAAGATAGAATGATTGCCAAAGTTGAACCCCTTGTGAACATGGGACAAATAAGCAGGGCCACTGTTCCACTCAATCTTTCTCTTGCTGTAGTTGCTGAACTTGACGATCTTACTGTTGGAGGACTCATCAATGGCTATGGAATTGAAGGAAGCTCCCATCTCTATGGCTTGTTCTCGGACACTGTTGTGGCATATGAGATTGTTCTAGCAGATGGCCAGGTTGTTAGAGCTACTAAAGATAATGAGTACTCAGATCTTTTCTATGCAATCCCATGGTCCCAAGGAACAATTGGTCTTCTTGTTGCTGCTGAGATTAAGCTCATACCCATTAAAGAGTATATGAAAGTCACATACAAACCCTTTAGAGGGACATTGAGAGAGATTGGACAAGCTTATATGGATTCTCTTGCCCCTAGAGATGGAGATCAAGATAACCCAGAAAAGGTTCCCGACTTTGTTGAAACTATGATTTATACCCCTTCTGAAGCTGTGGTCATGACAGGTAGATATGCTTCCAAAGAAGAGGCTAAAAAAAGGGGAAATGTGATAAATCAGATTGGTTGGTGGTTCAAACCATGGTTCTACCAGCATGCATCAACAGCACTCAAGAAAGGGGAGTTTGTTGAGTATATCCCTACAAGGGATTACTATCATAGGCACACCAGATCTTTGTATTGGGAGGGAAAGCTTATCCTTCCCTTTGCAGATCAATGGTGGTTCAGATTTTTATTGGGATGGATGATGCCTCCCAAAGTTTCTCTGCTCAAAGCCACTCAAGGTGAAGCTATCAGAAATTATTATCATGAAATGCATGTTATTCAAGACATGCTTGTTCCTCTTTACAAGGTTCCGGATGCTTTGGAGTGGGCTCATCGTGAGTTTGAGGTTTACCCTATTTGGCTCTGTCCACACAGAATCTTCAAGCTTCCAGTCAAAACCATGATATATCCCGAACCAGGATTCGAGTTACATCGTCGACAAGGCGACACACATTACGCTCAAATGTACACAGATGTTGGACTGTACTATGCACCAGGCCCTGTCCTGAGGGGCGAAGTTTTCGATGGTGCCGAGGCTGTTCGTAGAATGGAGAAATGGCTTCTAGAAAACCATGGATTCCAGGCTCTATATGCAGTATCAGAACTGAATGAGAAAGATTTCTGGAGGATGTTTGATGCTGAACTCTATGAACAATGCCGGAAGAAATATGGAGCTGTAGGAACCTTTATGACAGTCTATTACAAGTCCAAGAAAGGTCGAAAAACCGAGAAGGAAGTGAGGGAAGCTGAACAAGCTCATCTTGAAACTACTTATGCTGAATTGGAACAACCCAATTAA

Protein sequence

MSDLEVPLRPKRKKVWVDYFVQFRWILVIFVVLPISFSLYFFTYLGDVRSAWKSYKKRQKEHDENVKKVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDIDKDRMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFSDTVVAYEIVLADGQVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYKPFRGTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKEEAKKRGNVINQIGWWFKPWFYQHASTALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPFADQWWFRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHREFEVYPIWLCPHRIFKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGLYYAPGPVLRGEVFDGAEAVRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAELYEQCRKKYGAVGTFMTVYYKSKKGRKTEKEVREAEQAHLETTYAELEQPN
Homology
BLAST of CaUC09G162250 vs. NCBI nr
Match: XP_038898378.1 (delta(24)-sterol reductase [Benincasa hispida] >XP_038898379.1 delta(24)-sterol reductase [Benincasa hispida] >XP_038898380.1 delta(24)-sterol reductase [Benincasa hispida] >XP_038898381.1 delta(24)-sterol reductase [Benincasa hispida] >XP_038898382.1 delta(24)-sterol reductase [Benincasa hispida])

HSP 1 Score: 1157.5 bits (2993), Expect = 0.0e+00
Identity = 561/566 (99.12%), Postives = 563/566 (99.47%), Query Frame = 0

Query: 1   MSDLEVPLRPKRKKVWVDYFVQFRWILVIFVVLPISFSLYFFTYLGDVRSAWKSYKKRQK 60
           MSDL VPLRPKRKKVWVDYFVQFRWILVIFVVLPISFSLYFFTYLGDVRSAWKSYKKRQK
Sbjct: 1   MSDLVVPLRPKRKKVWVDYFVQFRWILVIFVVLPISFSLYFFTYLGDVRSAWKSYKKRQK 60

Query: 61  EHDENVKKVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI 120
           EHDENV+KVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI
Sbjct: 61  EHDENVQKVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI 120

Query: 121 DKDRMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS 180
           DKDRMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS
Sbjct: 121 DKDRMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS 180

Query: 181 DTVVAYEIVLADGQVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYK 240
           DTVVAYEIVLADGQVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYK
Sbjct: 181 DTVVAYEIVLADGQVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYK 240

Query: 241 PFRGTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKEEAKKR 300
           PFRGTL+EIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKEEAKKR
Sbjct: 241 PFRGTLKEIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKEEAKKR 300

Query: 301 GNVINQIGWWFKPWFYQHASTALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPFADQWW 360
           GNVINQIGWWFKPWFYQHASTALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPFADQWW
Sbjct: 301 GNVINQIGWWFKPWFYQHASTALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPFADQWW 360

Query: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHREFEVYPI 420
           FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHREFEVYPI
Sbjct: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHREFEVYPI 420

Query: 421 WLCPHRIFKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGLYYAPGPVLRGEVFDGAEA 480
           WLCPHRIFKLPVKTMIYPEPGFEL RRQGDTHYAQMYTDVGLYYAPGPVLRGEVFDGAEA
Sbjct: 421 WLCPHRIFKLPVKTMIYPEPGFELQRRQGDTHYAQMYTDVGLYYAPGPVLRGEVFDGAEA 480

Query: 481 VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAELYEQCRKKYGAVGTFMTVYYKSKKG 540
           VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDA LYEQCRKKYGAVGTFMTVYYKSKKG
Sbjct: 481 VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAGLYEQCRKKYGAVGTFMTVYYKSKKG 540

Query: 541 RKTEKEVREAEQAHLETTYAELEQPN 567
           RKTEKEVREAEQAHLETTYAELEQPN
Sbjct: 541 RKTEKEVREAEQAHLETTYAELEQPN 566

BLAST of CaUC09G162250 vs. NCBI nr
Match: KAG7014278.1 (Delta(24)-sterol reductase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1152.5 bits (2980), Expect = 0.0e+00
Identity = 554/566 (97.88%), Postives = 564/566 (99.65%), Query Frame = 0

Query: 1   MSDLEVPLRPKRKKVWVDYFVQFRWILVIFVVLPISFSLYFFTYLGDVRSAWKSYKKRQK 60
           MSDLEVPLRPKRKKVWVDYFVQFRWILVIFVVLPISFSLYFFTYLGDV+SAWKSY+KRQK
Sbjct: 1   MSDLEVPLRPKRKKVWVDYFVQFRWILVIFVVLPISFSLYFFTYLGDVKSAWKSYEKRQK 60

Query: 61  EHDENVKKVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI 120
           EHDENVKKVVKRLKQRNP KDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI
Sbjct: 61  EHDENVKKVVKRLKQRNPTKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI 120

Query: 121 DKDRMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS 180
           DK+RMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGL+NGYGIEGSSHLYGLFS
Sbjct: 121 DKERMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLVNGYGIEGSSHLYGLFS 180

Query: 181 DTVVAYEIVLADGQVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYK 240
           DTVVAYEIVLADGQVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYK
Sbjct: 181 DTVVAYEIVLADGQVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYK 240

Query: 241 PFRGTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKEEAKKR 300
           PF GTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVM+GRYASKEEAKK+
Sbjct: 241 PFIGTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMSGRYASKEEAKKK 300

Query: 301 GNVINQIGWWFKPWFYQHASTALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPFADQWW 360
           GNVINQIGWWFKPWFYQHA+TALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPFADQWW
Sbjct: 301 GNVINQIGWWFKPWFYQHAATALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPFADQWW 360

Query: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHREFEVYPI 420
           FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHREFEVYPI
Sbjct: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHREFEVYPI 420

Query: 421 WLCPHRIFKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGLYYAPGPVLRGEVFDGAEA 480
           WLCPHR+FKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGLYYAPGPVLRGEVFDGAEA
Sbjct: 421 WLCPHRLFKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGLYYAPGPVLRGEVFDGAEA 480

Query: 481 VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAELYEQCRKKYGAVGTFMTVYYKSKKG 540
           VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAELYEQCRKKYGAVGTFMTVYYK+KKG
Sbjct: 481 VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAELYEQCRKKYGAVGTFMTVYYKTKKG 540

Query: 541 RKTEKEVREAEQAHLETTYAELEQPN 567
           RKTEKEV+EAEQAHLETTYAELEQPN
Sbjct: 541 RKTEKEVKEAEQAHLETTYAELEQPN 566

BLAST of CaUC09G162250 vs. NCBI nr
Match: XP_023548025.1 (delta(24)-sterol reductase [Cucurbita pepo subsp. pepo] >XP_023548027.1 delta(24)-sterol reductase [Cucurbita pepo subsp. pepo] >XP_023548028.1 delta(24)-sterol reductase [Cucurbita pepo subsp. pepo] >XP_023548029.1 delta(24)-sterol reductase [Cucurbita pepo subsp. pepo] >XP_023548030.1 delta(24)-sterol reductase [Cucurbita pepo subsp. pepo] >XP_023548031.1 delta(24)-sterol reductase [Cucurbita pepo subsp. pepo] >XP_023548032.1 delta(24)-sterol reductase [Cucurbita pepo subsp. pepo] >XP_023548033.1 delta(24)-sterol reductase [Cucurbita pepo subsp. pepo] >XP_023548034.1 delta(24)-sterol reductase [Cucurbita pepo subsp. pepo] >XP_023548035.1 delta(24)-sterol reductase [Cucurbita pepo subsp. pepo] >XP_023548036.1 delta(24)-sterol reductase [Cucurbita pepo subsp. pepo] >XP_023548037.1 delta(24)-sterol reductase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1148.7 bits (2970), Expect = 0.0e+00
Identity = 553/566 (97.70%), Postives = 562/566 (99.29%), Query Frame = 0

Query: 1   MSDLEVPLRPKRKKVWVDYFVQFRWILVIFVVLPISFSLYFFTYLGDVRSAWKSYKKRQK 60
           MSDLEVPLRPKRKKVWVDYFVQFRWILVIFVVLPISFSLYFFTYLGDV+SAWKSY+KRQK
Sbjct: 1   MSDLEVPLRPKRKKVWVDYFVQFRWILVIFVVLPISFSLYFFTYLGDVKSAWKSYEKRQK 60

Query: 61  EHDENVKKVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI 120
           EHDENVKKVVKRLKQRNP KDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI
Sbjct: 61  EHDENVKKVVKRLKQRNPTKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI 120

Query: 121 DKDRMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS 180
           DK+RMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS
Sbjct: 121 DKERMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS 180

Query: 181 DTVVAYEIVLADGQVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYK 240
           DTVVAYEIVLADGQVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYK
Sbjct: 181 DTVVAYEIVLADGQVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYK 240

Query: 241 PFRGTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKEEAKKR 300
           PF GTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKEEAKK+
Sbjct: 241 PFIGTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKEEAKKK 300

Query: 301 GNVINQIGWWFKPWFYQHASTALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPFADQWW 360
           GNVINQIGWWFKPWFYQHA+TALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILP ADQWW
Sbjct: 301 GNVINQIGWWFKPWFYQHAATALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPLADQWW 360

Query: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHREFEVYPI 420
           FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDAL+WAHRE EVYPI
Sbjct: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALDWAHRELEVYPI 420

Query: 421 WLCPHRIFKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGLYYAPGPVLRGEVFDGAEA 480
           WLCPHR+FKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGLYYAPGPVLRGEVFDGAEA
Sbjct: 421 WLCPHRLFKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGLYYAPGPVLRGEVFDGAEA 480

Query: 481 VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAELYEQCRKKYGAVGTFMTVYYKSKKG 540
           VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAELYEQCRKKYGAVGTFMTVYYK+KKG
Sbjct: 481 VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAELYEQCRKKYGAVGTFMTVYYKTKKG 540

Query: 541 RKTEKEVREAEQAHLETTYAELEQPN 567
           RKTEKEV+EAEQAHLETTYAELEQPN
Sbjct: 541 RKTEKEVKEAEQAHLETTYAELEQPN 566

BLAST of CaUC09G162250 vs. NCBI nr
Match: XP_022953594.1 (delta(24)-sterol reductase [Cucurbita moschata] >XP_022953595.1 delta(24)-sterol reductase [Cucurbita moschata] >XP_022953596.1 delta(24)-sterol reductase [Cucurbita moschata] >XP_022953597.1 delta(24)-sterol reductase [Cucurbita moschata])

HSP 1 Score: 1148.3 bits (2969), Expect = 0.0e+00
Identity = 553/566 (97.70%), Postives = 563/566 (99.47%), Query Frame = 0

Query: 1   MSDLEVPLRPKRKKVWVDYFVQFRWILVIFVVLPISFSLYFFTYLGDVRSAWKSYKKRQK 60
           MSDLEVPLRPKRKKVWVDYFVQFRWILVIFVVLPISFSLYFFTYLGDV+SAWKSY+KRQK
Sbjct: 1   MSDLEVPLRPKRKKVWVDYFVQFRWILVIFVVLPISFSLYFFTYLGDVKSAWKSYEKRQK 60

Query: 61  EHDENVKKVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI 120
           EHDENVKKVVKRLKQRNP KDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI
Sbjct: 61  EHDENVKKVVKRLKQRNPTKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI 120

Query: 121 DKDRMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS 180
           DK+RMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS
Sbjct: 121 DKERMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS 180

Query: 181 DTVVAYEIVLADGQVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYK 240
           DTVVAYEIVLADGQ+VRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYK
Sbjct: 181 DTVVAYEIVLADGQLVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYK 240

Query: 241 PFRGTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKEEAKKR 300
           PF GTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVM+GRYASKEEAKK+
Sbjct: 241 PFIGTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMSGRYASKEEAKKK 300

Query: 301 GNVINQIGWWFKPWFYQHASTALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPFADQWW 360
           GNVINQIGWWFKPWFYQHA+TALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPFADQWW
Sbjct: 301 GNVINQIGWWFKPWFYQHAATALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPFADQWW 360

Query: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHREFEVYPI 420
           FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHREFEVYPI
Sbjct: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHREFEVYPI 420

Query: 421 WLCPHRIFKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGLYYAPGPVLRGEVFDGAEA 480
           WLCPHR+FKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGLYYAPGPVLRGEVFDGAEA
Sbjct: 421 WLCPHRLFKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGLYYAPGPVLRGEVFDGAEA 480

Query: 481 VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAELYEQCRKKYGAVGTFMTVYYKSKKG 540
           VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAELYEQCRKKYGAVGTFMTVYYK+KKG
Sbjct: 481 VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAELYEQCRKKYGAVGTFMTVYYKTKKG 540

Query: 541 RKTEKEVREAEQAHLETTYAELEQPN 567
           RKTEKEV+EAEQAHLETT AELEQPN
Sbjct: 541 RKTEKEVKEAEQAHLETTCAELEQPN 566

BLAST of CaUC09G162250 vs. NCBI nr
Match: XP_022960164.1 (delta(24)-sterol reductase-like [Cucurbita moschata] >XP_022960165.1 delta(24)-sterol reductase-like [Cucurbita moschata] >XP_022960166.1 delta(24)-sterol reductase-like [Cucurbita moschata] >XP_022960167.1 delta(24)-sterol reductase-like [Cucurbita moschata] >KAG7025620.1 Delta(24)-sterol reductase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1144.8 bits (2960), Expect = 0.0e+00
Identity = 549/566 (97.00%), Postives = 561/566 (99.12%), Query Frame = 0

Query: 1   MSDLEVPLRPKRKKVWVDYFVQFRWILVIFVVLPISFSLYFFTYLGDVRSAWKSYKKRQK 60
           MSDLEVPLRPKRKKVWVDYFV+FRWILVIFVVLPISF LYFFTYLGDVRSAWKSYKKRQK
Sbjct: 1   MSDLEVPLRPKRKKVWVDYFVKFRWILVIFVVLPISFCLYFFTYLGDVRSAWKSYKKRQK 60

Query: 61  EHDENVKKVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI 120
           EHDENV+KVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI
Sbjct: 61  EHDENVEKVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI 120

Query: 121 DKDRMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS 180
           D+++MIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS
Sbjct: 121 DQEKMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS 180

Query: 181 DTVVAYEIVLADGQVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYK 240
           DTVVAYE+VLADGQVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYK
Sbjct: 181 DTVVAYEVVLADGQVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYK 240

Query: 241 PFRGTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKEEAKKR 300
           PFRGTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASK EAK++
Sbjct: 241 PFRGTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKVEAKRK 300

Query: 301 GNVINQIGWWFKPWFYQHASTALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPFADQWW 360
           GNVINQIGWWFKPWFYQHASTALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPFADQWW
Sbjct: 301 GNVINQIGWWFKPWFYQHASTALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPFADQWW 360

Query: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHREFEVYPI 420
           FRFL GWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHRE EVYP+
Sbjct: 361 FRFLFGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHRELEVYPV 420

Query: 421 WLCPHRIFKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGLYYAPGPVLRGEVFDGAEA 480
           WLCPHR+FKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGLYYAPGP+LRGEVFDGAEA
Sbjct: 421 WLCPHRLFKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGLYYAPGPILRGEVFDGAEA 480

Query: 481 VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAELYEQCRKKYGAVGTFMTVYYKSKKG 540
           VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAE YEQCRKKYGAVGTFMTVYYKSKKG
Sbjct: 481 VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAEHYEQCRKKYGAVGTFMTVYYKSKKG 540

Query: 541 RKTEKEVREAEQAHLETTYAELEQPN 567
           RKTEKEV+EAEQAHLETTYAELEQPN
Sbjct: 541 RKTEKEVKEAEQAHLETTYAELEQPN 566

BLAST of CaUC09G162250 vs. ExPASy Swiss-Prot
Match: P93472 (Delta(24)-sterol reductase OS=Pisum sativum OX=3888 GN=DIM PE=1 SV=1)

HSP 1 Score: 1010.4 bits (2611), Expect = 8.2e-294
Identity = 476/565 (84.25%), Postives = 522/565 (92.39%), Query Frame = 0

Query: 1   MSDLEVPLRPKRKKVWVDYFVQFRWILVIFVVLPISFSLYFFTYLGDVRSAWKSYKKRQK 60
           MSDLE PLRPKRKK+WVDYFV+FRWILVIFVVLPISF+LYF TYLGDVRS WKS+K RQK
Sbjct: 1   MSDLEAPLRPKRKKIWVDYFVKFRWILVIFVVLPISFTLYFLTYLGDVRSEWKSFKTRQK 60

Query: 61  EHDENVKKVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI 120
           EHDENV+KVV RLK+RNP+KDGLVCTARKPW+AVGMRNVDYKRARHFEVDLS FRNILDI
Sbjct: 61  EHDENVQKVVNRLKKRNPSKDGLVCTARKPWVAVGMRNVDYKRARHFEVDLSPFRNILDI 120

Query: 121 DKDRMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS 180
           DK+RMIA+VEPLVNMGQI+R TVP+NL+LAVVAELDDLTVGGLINGYGIEGSSH YGLFS
Sbjct: 121 DKERMIARVEPLVNMGQITRVTVPMNLALAVVAELDDLTVGGLINGYGIEGSSHKYGLFS 180

Query: 181 DTVVAYEIVLADGQVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYK 240
           DTVVA+EI+LADG +V+ATKDNEYSDLFYAIPWSQGT+GLLVAAE+KLIPIKEYMK+TYK
Sbjct: 181 DTVVAFEIILADGSLVKATKDNEYSDLFYAIPWSQGTLGLLVAAEVKLIPIKEYMKLTYK 240

Query: 241 PFRGTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKEEAKKR 300
           P  G L++I QAY DS APRDGDQDN EKVPDFVETMIY+P+ AV MTGRYASKEEAKK+
Sbjct: 241 PVVGNLKDIAQAYSDSFAPRDGDQDNDEKVPDFVETMIYSPTRAVCMTGRYASKEEAKKK 300

Query: 301 GNVINQIGWWFKPWFYQHASTALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPFADQWW 360
           GN IN +GWW+K WFYQHA TALKKG FVEYIPTR+YYHRHTR LYWEGKLILPF DQ+ 
Sbjct: 301 GNKINNVGWWYKTWFYQHAETALKKGLFVEYIPTREYYHRHTRCLYWEGKLILPFGDQFS 360

Query: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHREFEVYPI 420
           FRFL GW+MPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKV DALEW+ RE E+YPI
Sbjct: 361 FRFLFGWLMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVGDALEWSDREMEIYPI 420

Query: 421 WLCPHRIFKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGLYYAPGPVLRGEVFDGAEA 480
           WLCPH++FKLP+KTMIYPE GFEL RRQGDT  AQM+TDVG+YYAPGPVLRGEVFDGAEA
Sbjct: 421 WLCPHKLFKLPIKTMIYPEAGFELQRRQGDTQNAQMFTDVGVYYAPGPVLRGEVFDGAEA 480

Query: 481 VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAELYEQCRKKYGAVGTFMTVYYKSKKG 540
           VR+ME W++ENH FQ  YAVSELNEK+FWRMFDA LYE CR+KYGAVGTFM+VYYK KKG
Sbjct: 481 VRKMESWMIENHCFQPQYAVSELNEKNFWRMFDAGLYEHCRRKYGAVGTFMSVYYKCKKG 540

Query: 541 RKTEKEVREAEQAHLETTYAELEQP 566
           RKTEKEVREAEQAHL+T Y E++QP
Sbjct: 541 RKTEKEVREAEQAHLDTAYPEVDQP 565

BLAST of CaUC09G162250 vs. ExPASy Swiss-Prot
Match: Q39085 (Delta(24)-sterol reductase OS=Arabidopsis thaliana OX=3702 GN=DIM PE=1 SV=2)

HSP 1 Score: 947.2 bits (2447), Expect = 8.5e-275
Identity = 446/564 (79.08%), Postives = 504/564 (89.36%), Query Frame = 0

Query: 1   MSDLEVPL-RPKRKKVWVDYFVQFRWILVIFVVLPISFSLYFFTYLGDVRSAWKSYKKRQ 60
           MSDL+ PL RPKRKK WVDYFV+FRWI+VIF+VLP S + YF  YLGD+ S  KS++KRQ
Sbjct: 1   MSDLQTPLVRPKRKKTWVDYFVKFRWIIVIFIVLPFSATFYFLIYLGDMWSESKSFEKRQ 60

Query: 61  KEHDENVKKVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILD 120
           KEHDENVKKV+KRLK R+ +KDGLVCTARKPWIAVGMRNVDYKRARHFEVDL  FRNIL+
Sbjct: 61  KEHDENVKKVIKRLKGRDASKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLGEFRNILE 120

Query: 121 IDKDRMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLF 180
           I+K++M A+VEPLVNMGQISRATVP+NLSLAVVAELDDLTVGGLINGYGIEGSSH+YGLF
Sbjct: 121 INKEKMTARVEPLVNMGQISRATVPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLF 180

Query: 181 SDTVVAYEIVLADGQVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTY 240
           +DTV AYEIVLA G++VRAT+DNEYSDL+YAIPWSQGT+GLLVAAEI+LI +KEYM++TY
Sbjct: 181 ADTVEAYEIVLAGGELVRATRDNEYSDLYYAIPWSQGTLGLLVAAEIRLIKVKEYMRLTY 240

Query: 241 KPFRGTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKEEAKK 300
            P +G L+ + Q Y+DS AP+DGD+    K+PDFVE M+Y P+E V+M G YASKEEAKK
Sbjct: 241 IPVKGDLQALAQGYIDSFAPKDGDK---SKIPDFVEGMVYNPTEGVMMVGTYASKEEAKK 300

Query: 301 RGNVINQIGWWFKPWFYQHASTALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPFADQW 360
           +GN IN +GWWFKPWFYQHA TALKKG+FVEYIPTR+YYHRHTR LYWEGKLILPF DQ+
Sbjct: 301 KGNKINNVGWWFKPWFYQHAQTALKKGQFVEYIPTREYYHRHTRCLYWEGKLILPFGDQF 360

Query: 361 WFRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHREFEVYP 420
           WFR+LLGW+MPPKVSLLKATQGEAIRNYYH+MHVIQDMLVPLYKV DALEW HRE EVYP
Sbjct: 361 WFRYLLGWLMPPKVSLLKATQGEAIRNYYHDMHVIQDMLVPLYKVGDALEWVHREMEVYP 420

Query: 421 IWLCPHRIFKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGLYYAPGPVLRGEVFDGAE 480
           IWLCPH++FK P+K  IYPEPGFE   RQGDT  AQMYTDVG+YYAPG VLRGE FDG+E
Sbjct: 421 IWLCPHKLFKQPIKGQIYPEPGFEYENRQGDTEDAQMYTDVGVYYAPGCVLRGEEFDGSE 480

Query: 481 AVRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAELYEQCRKKYGAVGTFMTVYYKSKK 540
           AVRRMEKWL+ENHGFQ  YAVSEL+EK FWRMF+ ELYE+CRKKY A+GTFM+VYYKSKK
Sbjct: 481 AVRRMEKWLIENHGFQPQYAVSELDEKSFWRMFNGELYEECRKKYRAIGTFMSVYYKSKK 540

Query: 541 GRKTEKEVREAEQAHLETTYAELE 564
           GRKTEKEVREAEQAHLET YAE +
Sbjct: 541 GRKTEKEVREAEQAHLETAYAEAD 561

BLAST of CaUC09G162250 vs. ExPASy Swiss-Prot
Match: Q15392 (Delta(24)-sterol reductase OS=Homo sapiens OX=9606 GN=DHCR24 PE=1 SV=2)

HSP 1 Score: 386.0 bits (990), Expect = 7.5e-106
Identity = 218/525 (41.52%), Postives = 310/525 (59.05%), Query Frame = 0

Query: 24  RWILVIFVVLPIS--FSLYFFTYLGDVRSAWKSYK--KRQKEHDENVKKVVKRLKQ-RNP 83
           RW+ V   +LP+S  F +Y++        AW  +K     + H++ V+ + K++++ +  
Sbjct: 31  RWVFVCLFLLPLSLIFDIYYYV------RAWVVFKLSSAPRLHEQRVRDIQKQVREWKEQ 90

Query: 84  AKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDIDKDRMIAKVEPLVNMGQI 143
                +CT R  W+ V +R   YK+  H  + ++   +IL++D  + I +VEPLV MGQ+
Sbjct: 91  GSKTFMCTGRPGWLTVSLRVGKYKKT-HKNIMIN-LMDILEVDTKKQIVRVEPLVTMGQV 150

Query: 144 SRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFSDTVVAYEIVLADGQVVRA 203
           +     +  +L V+ ELDDLTVGGLI G GIE SSH YGLF     AYE+VLADG  VR 
Sbjct: 151 TALLTSIGWTLPVLPELDDLTVGGLIMGTGIESSSHKYGLFQHICTAYELVLADGSFVRC 210

Query: 204 TKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYKPFRGTLREIGQAYMDSLA 263
           T  +E SDLFYA+PWS GT+G LVAAEI++IP K+Y+K+ ++P RG      +A      
Sbjct: 211 T-PSENSDLFYAVPWSCGTLGFLVAAEIRIIPAKKYVKLRFEPVRGL-----EAICAKFT 270

Query: 264 PRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKEEAKKRGNVINQIGWWFKPWFYQH 323
                Q+N      FVE ++Y+  EAV+MTG    + E  K    +N IG ++KPWF++H
Sbjct: 271 HESQRQEN-----HFVEGLLYSLDEAVIMTGVMTDEAEPSK----LNSIGNYYKPWFFKH 330

Query: 324 ASTALKKG-EFVEYIPTRDYYHRHTRSLYWEGKLILPFADQWWFRFLLGWMMPPKVSLLK 383
               LK   E +EYIP R YYHRHTRS++WE + I+PF +   FR+L GWM+PPK+SLLK
Sbjct: 331 VENYLKTNREGLEYIPLRHYYHRHTRSIFWELQDIIPFGNNPIFRYLFGWMVPPKISLLK 390

Query: 384 ATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHREFEVYPIWLCPHRIFKLPVKTMIY 443
            TQGE +R  Y + HV+QDMLVP+  +  AL     +  VYPIWLCP   F LP      
Sbjct: 391 LTQGETLRKLYEQHHVVQDMLVPMKCLQQALHTFQNDIHVYPIWLCP---FILP------ 450

Query: 444 PEPGFELHRRQGDTHYAQMYTDVGLYYAPGPVLRGEVFDGAEAVRRMEKWLLENHGFQAL 503
            +PG  +H +  +   A++Y D+G Y  P    R + F+    +R++EK++   HGFQ L
Sbjct: 451 SQPGL-VHPKGNE---AELYIDIGAYGEP----RVKHFEARSCMRQLEKFVRSVHGFQML 510

Query: 504 YAVSELNEKDFWRMFDAELYEQCRKKYGAVGTFMTVYYKSKKGRK 543
           YA   +N ++FW MFD  LY + R+K G    F  VY K  K  +
Sbjct: 511 YADCYMNREEFWEMFDGSLYHKLREKLGCQDAFPEVYDKICKAAR 515

BLAST of CaUC09G162250 vs. ExPASy Swiss-Prot
Match: Q8VCH6 (Delta(24)-sterol reductase OS=Mus musculus OX=10090 GN=Dhcr24 PE=1 SV=1)

HSP 1 Score: 384.4 bits (986), Expect = 2.2e-105
Identity = 218/525 (41.52%), Postives = 310/525 (59.05%), Query Frame = 0

Query: 24  RWILVIFVVLPIS--FSLYFFTYLGDVRSAWKSYK--KRQKEHDENVKKVVKRLKQ-RNP 83
           RW+ V   +LP+S  F +Y++        AW  +K     + H++ V+ + K++++ +  
Sbjct: 31  RWVFVCLFLLPLSLIFDIYYYV------RAWVVFKLSSAPRLHEQRVRDIQKQVREWKEQ 90

Query: 84  AKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDIDKDRMIAKVEPLVNMGQI 143
                +CT R  W+ V +R   YK+  H  + ++   +IL++D  + I +VEPLV+MGQ+
Sbjct: 91  GSKTFMCTGRPGWLTVSLRVGKYKKT-HKNIMIN-LMDILEVDTKKQIVRVEPLVSMGQV 150

Query: 144 SRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFSDTVVAYEIVLADGQVVRA 203
           +     +  +L V+ ELDDLTVGGLI G GIE SSH YGLF     AYE++LADG  VR 
Sbjct: 151 TALLNSIGWTLPVLPELDDLTVGGLIMGTGIESSSHKYGLFQHICTAYELILADGSFVRC 210

Query: 204 TKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYKPFRGTLREIGQAYMDSLA 263
           T  +E SDLFYA+PWS GT+G LVAAEI++IP K+Y+K+ ++P RG      +A  +   
Sbjct: 211 T-PSENSDLFYAVPWSCGTLGFLVAAEIRIIPAKKYVKLRFEPVRGL-----EAICEKFT 270

Query: 264 PRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKEEAKKRGNVINQIGWWFKPWFYQH 323
                 +N      FVE ++Y+  EAV+MTG      E  K    +N IG ++KPWF++H
Sbjct: 271 RESQRLEN-----HFVEGLLYSLDEAVIMTGVMTDDVEPSK----LNSIGSYYKPWFFKH 330

Query: 324 ASTALKKG-EFVEYIPTRDYYHRHTRSLYWEGKLILPFADQWWFRFLLGWMMPPKVSLLK 383
               LK   E +EYIP R YYHRHTRS++WE + I+PF +   FR+L GWM+PPK+SLLK
Sbjct: 331 VENYLKTNREGLEYIPLRHYYHRHTRSIFWELQDIIPFGNNPIFRYLFGWMVPPKISLLK 390

Query: 384 ATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHREFEVYPIWLCPHRIFKLPVKTMIY 443
            TQGE +R  Y + HV+QDMLVP+  +  AL     +  VYPIWLCP   F LP      
Sbjct: 391 LTQGETLRKLYEQHHVVQDMLVPMKCMSQALHTFQNDIHVYPIWLCP---FILP------ 450

Query: 444 PEPGFELHRRQGDTHYAQMYTDVGLYYAPGPVLRGEVFDGAEAVRRMEKWLLENHGFQAL 503
            +PG  L   +GD   A++Y D+G Y  P    R + F+    +R++EK++   HGFQ L
Sbjct: 451 SQPG--LVHPKGDE--AELYVDIGAYGEP----RVKHFEARSCMRQLEKFVRSVHGFQML 510

Query: 504 YAVSELNEKDFWRMFDAELYEQCRKKYGAVGTFMTVYYKSKKGRK 543
           YA   +N ++FW MFD  LY + RK+ G    F  VY K  K  +
Sbjct: 511 YADCYMNREEFWEMFDGSLYHKLRKQLGCQDAFPEVYDKICKAAR 515

BLAST of CaUC09G162250 vs. ExPASy Swiss-Prot
Match: Q60HC5 (Delta(24)-sterol reductase OS=Macaca fascicularis OX=9541 GN=DHCR24 PE=2 SV=2)

HSP 1 Score: 384.0 bits (985), Expect = 2.9e-105
Identity = 217/525 (41.33%), Postives = 310/525 (59.05%), Query Frame = 0

Query: 24  RWILVIFVVLPIS--FSLYFFTYLGDVRSAWKSYK--KRQKEHDENVKKVVKRLKQ-RNP 83
           RW+ V   +LP+S  F +Y++        AW  +K     + H++ V+ + K++++ +  
Sbjct: 31  RWVFVCLFLLPLSLIFDIYYYV------RAWVVFKLSSAPRLHEQRVRDIQKQVREWKEQ 90

Query: 84  AKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDIDKDRMIAKVEPLVNMGQI 143
                +CT R  W+ V +R   YK+  H  + ++   +IL++D  + I +VEPLV MGQ+
Sbjct: 91  GSKTFMCTGRPGWLTVSLRVGKYKKT-HKNIMIN-LMDILEVDTKKQIVRVEPLVTMGQV 150

Query: 144 SRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFSDTVVAYEIVLADGQVVRA 203
           +     +  +L V+ ELDDLTVGGLI G GIE SSH YGLF     AYE+VLADG  VR 
Sbjct: 151 TALLTSIGWTLPVLPELDDLTVGGLIMGTGIESSSHKYGLFQHICTAYELVLADGSFVRC 210

Query: 204 TKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYKPFRGTLREIGQAYMDSLA 263
           T  +E SDLFYA+PWS GT+G LVAAEI++IP K+Y+K+ ++P +G      +A      
Sbjct: 211 T-PSENSDLFYAVPWSCGTLGFLVAAEIRIIPAKKYVKLRFEPVQGL-----EAICAKFT 270

Query: 264 PRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKEEAKKRGNVINQIGWWFKPWFYQH 323
                Q+N      FVE ++Y+  EAV+MTG    + E  K    +N IG ++KPWF++H
Sbjct: 271 HESQRQEN-----HFVEGLLYSLDEAVIMTGVMTDEVEPSK----LNSIGNYYKPWFFKH 330

Query: 324 ASTALKKG-EFVEYIPTRDYYHRHTRSLYWEGKLILPFADQWWFRFLLGWMMPPKVSLLK 383
               LK   E +EYIP R YYHRHTRS++WE + I+PF +   FR+L GWM+PPK+SLLK
Sbjct: 331 VENYLKTNREGLEYIPLRHYYHRHTRSIFWELQDIIPFGNNPIFRYLFGWMVPPKISLLK 390

Query: 384 ATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHREFEVYPIWLCPHRIFKLPVKTMIY 443
            TQGE +R  Y + HV+QDMLVP+  +  AL     +  VYPIWLCP   F LP      
Sbjct: 391 LTQGETLRKLYEQHHVVQDMLVPMKCLQQALHTFQNDIHVYPIWLCP---FILP------ 450

Query: 444 PEPGFELHRRQGDTHYAQMYTDVGLYYAPGPVLRGEVFDGAEAVRRMEKWLLENHGFQAL 503
            +PG  +H +  +   A++Y D+G Y  P    R + F+    +R++EK++   HGFQ L
Sbjct: 451 SQPGL-VHPKGNE---AELYIDIGAYGEP----RVKHFEARSCMRQLEKFVRSVHGFQML 510

Query: 504 YAVSELNEKDFWRMFDAELYEQCRKKYGAVGTFMTVYYKSKKGRK 543
           YA   +N ++FW MFD  LY + R+K G    F  VY K  K  +
Sbjct: 511 YADCYMNREEFWEMFDGSLYHKLREKLGCQDAFPEVYDKICKAAR 515

BLAST of CaUC09G162250 vs. ExPASy TrEMBL
Match: A0A6J1GQ50 (Delta(24)-sterol reductase OS=Cucurbita moschata OX=3662 GN=LOC111456086 PE=4 SV=1)

HSP 1 Score: 1148.3 bits (2969), Expect = 0.0e+00
Identity = 553/566 (97.70%), Postives = 563/566 (99.47%), Query Frame = 0

Query: 1   MSDLEVPLRPKRKKVWVDYFVQFRWILVIFVVLPISFSLYFFTYLGDVRSAWKSYKKRQK 60
           MSDLEVPLRPKRKKVWVDYFVQFRWILVIFVVLPISFSLYFFTYLGDV+SAWKSY+KRQK
Sbjct: 1   MSDLEVPLRPKRKKVWVDYFVQFRWILVIFVVLPISFSLYFFTYLGDVKSAWKSYEKRQK 60

Query: 61  EHDENVKKVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI 120
           EHDENVKKVVKRLKQRNP KDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI
Sbjct: 61  EHDENVKKVVKRLKQRNPTKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI 120

Query: 121 DKDRMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS 180
           DK+RMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS
Sbjct: 121 DKERMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS 180

Query: 181 DTVVAYEIVLADGQVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYK 240
           DTVVAYEIVLADGQ+VRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYK
Sbjct: 181 DTVVAYEIVLADGQLVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYK 240

Query: 241 PFRGTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKEEAKKR 300
           PF GTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVM+GRYASKEEAKK+
Sbjct: 241 PFIGTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMSGRYASKEEAKKK 300

Query: 301 GNVINQIGWWFKPWFYQHASTALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPFADQWW 360
           GNVINQIGWWFKPWFYQHA+TALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPFADQWW
Sbjct: 301 GNVINQIGWWFKPWFYQHAATALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPFADQWW 360

Query: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHREFEVYPI 420
           FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHREFEVYPI
Sbjct: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHREFEVYPI 420

Query: 421 WLCPHRIFKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGLYYAPGPVLRGEVFDGAEA 480
           WLCPHR+FKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGLYYAPGPVLRGEVFDGAEA
Sbjct: 421 WLCPHRLFKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGLYYAPGPVLRGEVFDGAEA 480

Query: 481 VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAELYEQCRKKYGAVGTFMTVYYKSKKG 540
           VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAELYEQCRKKYGAVGTFMTVYYK+KKG
Sbjct: 481 VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAELYEQCRKKYGAVGTFMTVYYKTKKG 540

Query: 541 RKTEKEVREAEQAHLETTYAELEQPN 567
           RKTEKEV+EAEQAHLETT AELEQPN
Sbjct: 541 RKTEKEVKEAEQAHLETTCAELEQPN 566

BLAST of CaUC09G162250 vs. ExPASy TrEMBL
Match: A0A6J1H6V4 (Delta(24)-sterol reductase OS=Cucurbita moschata OX=3662 GN=LOC111460987 PE=4 SV=1)

HSP 1 Score: 1144.8 bits (2960), Expect = 0.0e+00
Identity = 549/566 (97.00%), Postives = 561/566 (99.12%), Query Frame = 0

Query: 1   MSDLEVPLRPKRKKVWVDYFVQFRWILVIFVVLPISFSLYFFTYLGDVRSAWKSYKKRQK 60
           MSDLEVPLRPKRKKVWVDYFV+FRWILVIFVVLPISF LYFFTYLGDVRSAWKSYKKRQK
Sbjct: 1   MSDLEVPLRPKRKKVWVDYFVKFRWILVIFVVLPISFCLYFFTYLGDVRSAWKSYKKRQK 60

Query: 61  EHDENVKKVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI 120
           EHDENV+KVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI
Sbjct: 61  EHDENVEKVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI 120

Query: 121 DKDRMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS 180
           D+++MIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS
Sbjct: 121 DQEKMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS 180

Query: 181 DTVVAYEIVLADGQVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYK 240
           DTVVAYE+VLADGQVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYK
Sbjct: 181 DTVVAYEVVLADGQVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYK 240

Query: 241 PFRGTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKEEAKKR 300
           PFRGTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASK EAK++
Sbjct: 241 PFRGTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKVEAKRK 300

Query: 301 GNVINQIGWWFKPWFYQHASTALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPFADQWW 360
           GNVINQIGWWFKPWFYQHASTALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPFADQWW
Sbjct: 301 GNVINQIGWWFKPWFYQHASTALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPFADQWW 360

Query: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHREFEVYPI 420
           FRFL GWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHRE EVYP+
Sbjct: 361 FRFLFGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHRELEVYPV 420

Query: 421 WLCPHRIFKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGLYYAPGPVLRGEVFDGAEA 480
           WLCPHR+FKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGLYYAPGP+LRGEVFDGAEA
Sbjct: 421 WLCPHRLFKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGLYYAPGPILRGEVFDGAEA 480

Query: 481 VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAELYEQCRKKYGAVGTFMTVYYKSKKG 540
           VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAE YEQCRKKYGAVGTFMTVYYKSKKG
Sbjct: 481 VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAEHYEQCRKKYGAVGTFMTVYYKSKKG 540

Query: 541 RKTEKEVREAEQAHLETTYAELEQPN 567
           RKTEKEV+EAEQAHLETTYAELEQPN
Sbjct: 541 RKTEKEVKEAEQAHLETTYAELEQPN 566

BLAST of CaUC09G162250 vs. ExPASy TrEMBL
Match: A0A6J1JUW6 (Delta(24)-sterol reductase OS=Cucurbita maxima OX=3661 GN=LOC111488060 PE=4 SV=1)

HSP 1 Score: 1144.4 bits (2959), Expect = 0.0e+00
Identity = 551/566 (97.35%), Postives = 561/566 (99.12%), Query Frame = 0

Query: 1   MSDLEVPLRPKRKKVWVDYFVQFRWILVIFVVLPISFSLYFFTYLGDVRSAWKSYKKRQK 60
           MSDLEVPLRPKRKKVWVDYFVQFRWILVIFVVLPISFSLYFFTYLGDV+SAWKSY+KRQK
Sbjct: 1   MSDLEVPLRPKRKKVWVDYFVQFRWILVIFVVLPISFSLYFFTYLGDVKSAWKSYEKRQK 60

Query: 61  EHDENVKKVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI 120
           EHDENVKKVVKRLKQRNP KDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI
Sbjct: 61  EHDENVKKVVKRLKQRNPTKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI 120

Query: 121 DKDRMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS 180
           DK+RMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS
Sbjct: 121 DKERMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS 180

Query: 181 DTVVAYEIVLADGQVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYK 240
           DTVVAYEIVLA+GQVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYK
Sbjct: 181 DTVVAYEIVLANGQVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYK 240

Query: 241 PFRGTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKEEAKKR 300
           PF GTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVM GRYASKEEAK +
Sbjct: 241 PFIGTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMKGRYASKEEAKIK 300

Query: 301 GNVINQIGWWFKPWFYQHASTALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPFADQWW 360
           GNVINQIGWWFKPWFYQHA+TALKKGEFVEY+PTRDYYHRHTRSLYWEGKLILPFADQWW
Sbjct: 301 GNVINQIGWWFKPWFYQHAATALKKGEFVEYVPTRDYYHRHTRSLYWEGKLILPFADQWW 360

Query: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHREFEVYPI 420
           FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHRE EVYPI
Sbjct: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHRELEVYPI 420

Query: 421 WLCPHRIFKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGLYYAPGPVLRGEVFDGAEA 480
           WLCPHR+FKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGLYYAPGPVLRGEVFDGAEA
Sbjct: 421 WLCPHRLFKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGLYYAPGPVLRGEVFDGAEA 480

Query: 481 VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAELYEQCRKKYGAVGTFMTVYYKSKKG 540
           VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAELYEQCRKKYGAVGTFMTVYYK+KKG
Sbjct: 481 VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAELYEQCRKKYGAVGTFMTVYYKTKKG 540

Query: 541 RKTEKEVREAEQAHLETTYAELEQPN 567
           RKTEKEV+EAEQAHLETTYAELEQPN
Sbjct: 541 RKTEKEVKEAEQAHLETTYAELEQPN 566

BLAST of CaUC09G162250 vs. ExPASy TrEMBL
Match: A0A6J1KZQ6 (Delta(24)-sterol reductase OS=Cucurbita maxima OX=3661 GN=LOC111497787 PE=4 SV=1)

HSP 1 Score: 1144.4 bits (2959), Expect = 0.0e+00
Identity = 548/566 (96.82%), Postives = 561/566 (99.12%), Query Frame = 0

Query: 1   MSDLEVPLRPKRKKVWVDYFVQFRWILVIFVVLPISFSLYFFTYLGDVRSAWKSYKKRQK 60
           MSDLEVPLRPKRKKVWVDYFV+FRWILVIFVVLPISF LYFFTYLGDVRSAWKSYKKRQK
Sbjct: 1   MSDLEVPLRPKRKKVWVDYFVKFRWILVIFVVLPISFCLYFFTYLGDVRSAWKSYKKRQK 60

Query: 61  EHDENVKKVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI 120
           EHDENV+KVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI
Sbjct: 61  EHDENVEKVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI 120

Query: 121 DKDRMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS 180
           D+++MIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS
Sbjct: 121 DQEKMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS 180

Query: 181 DTVVAYEIVLADGQVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYK 240
           DTVVAYE+VLADGQVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYK
Sbjct: 181 DTVVAYEVVLADGQVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYK 240

Query: 241 PFRGTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKEEAKKR 300
           PFRGTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASK EAK++
Sbjct: 241 PFRGTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKVEAKRK 300

Query: 301 GNVINQIGWWFKPWFYQHASTALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPFADQWW 360
           GNVINQIGWWFKPWFYQHASTALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPFADQWW
Sbjct: 301 GNVINQIGWWFKPWFYQHASTALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPFADQWW 360

Query: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHREFEVYPI 420
           FRFL GWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHRE EVYP+
Sbjct: 361 FRFLFGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHRELEVYPV 420

Query: 421 WLCPHRIFKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGLYYAPGPVLRGEVFDGAEA 480
           WLCPHR+FKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGLYYAPGP+LRGE+FDGAEA
Sbjct: 421 WLCPHRLFKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGLYYAPGPILRGEIFDGAEA 480

Query: 481 VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAELYEQCRKKYGAVGTFMTVYYKSKKG 540
           VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAE YEQCRKKYGAVGTFMTVYYKSKKG
Sbjct: 481 VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAEHYEQCRKKYGAVGTFMTVYYKSKKG 540

Query: 541 RKTEKEVREAEQAHLETTYAELEQPN 567
           RKTEKEV+EAEQAHLETTYAELEQPN
Sbjct: 541 RKTEKEVKEAEQAHLETTYAELEQPN 566

BLAST of CaUC09G162250 vs. ExPASy TrEMBL
Match: A0A1S3CE88 (Delta(24)-sterol reductase OS=Cucumis melo OX=3656 GN=LOC103499976 PE=4 SV=1)

HSP 1 Score: 1133.6 bits (2931), Expect = 0.0e+00
Identity = 543/566 (95.94%), Postives = 558/566 (98.59%), Query Frame = 0

Query: 1   MSDLEVPLRPKRKKVWVDYFVQFRWILVIFVVLPISFSLYFFTYLGDVRSAWKSYKKRQK 60
           MSDL VPLRPKRKKVWVDYFVQFRWILVIFVVLPISFSLYFFTYLGDVRSAWKSYKKRQK
Sbjct: 1   MSDLNVPLRPKRKKVWVDYFVQFRWILVIFVVLPISFSLYFFTYLGDVRSAWKSYKKRQK 60

Query: 61  EHDENVKKVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI 120
           EHDENV+KVVKRLKQRNPAKDGL+CTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI
Sbjct: 61  EHDENVQKVVKRLKQRNPAKDGLICTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI 120

Query: 121 DKDRMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFS 180
           DKDRMIAKVEPLVNMGQISRATVPLNL+LAVV ELDDLTVGGLINGYGIEGSSHLYGLFS
Sbjct: 121 DKDRMIAKVEPLVNMGQISRATVPLNLALAVVPELDDLTVGGLINGYGIEGSSHLYGLFS 180

Query: 181 DTVVAYEIVLADGQVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTYK 240
           DTVVAYEIVLADG+VVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLI +KEYMKVTYK
Sbjct: 181 DTVVAYEIVLADGRVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIHVKEYMKVTYK 240

Query: 241 PFRGTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKEEAKKR 300
           PFRGTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVM+GRYASKEEAKK+
Sbjct: 241 PFRGTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMSGRYASKEEAKKK 300

Query: 301 GNVINQIGWWFKPWFYQHASTALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPFADQWW 360
           GNVINQIGWWFKPWFYQHASTALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPFADQWW
Sbjct: 301 GNVINQIGWWFKPWFYQHASTALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPFADQWW 360

Query: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHREFEVYPI 420
           FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMH+IQDML+PLYKVPDALEWAHREFEVYP+
Sbjct: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHIIQDMLIPLYKVPDALEWAHREFEVYPV 420

Query: 421 WLCPHRIFKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGLYYAPGPVLRGEVFDGAEA 480
           WLCPHRIFKLPVKTMI+PEPGFEL  RQGDT YAQMYTDVGLYYAPGPVLRGE FDGAEA
Sbjct: 421 WLCPHRIFKLPVKTMIFPEPGFELQSRQGDTKYAQMYTDVGLYYAPGPVLRGEAFDGAEA 480

Query: 481 VRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAELYEQCRKKYGAVGTFMTVYYKSKKG 540
           VRRMEKWLLENHGFQALYAVSELNEKDFW+M+DA LYEQCRKKYGAVGTFMTVYYK+KKG
Sbjct: 481 VRRMEKWLLENHGFQALYAVSELNEKDFWKMYDAGLYEQCRKKYGAVGTFMTVYYKTKKG 540

Query: 541 RKTEKEVREAEQAHLETTYAELEQPN 567
           RKTEKEVREAEQAHLETTYAE+EQPN
Sbjct: 541 RKTEKEVREAEQAHLETTYAEMEQPN 566

BLAST of CaUC09G162250 vs. TAIR 10
Match: AT3G19820.1 (cell elongation protein / DWARF1 / DIMINUTO (DIM) )

HSP 1 Score: 947.2 bits (2447), Expect = 6.0e-276
Identity = 446/564 (79.08%), Postives = 504/564 (89.36%), Query Frame = 0

Query: 1   MSDLEVPL-RPKRKKVWVDYFVQFRWILVIFVVLPISFSLYFFTYLGDVRSAWKSYKKRQ 60
           MSDL+ PL RPKRKK WVDYFV+FRWI+VIF+VLP S + YF  YLGD+ S  KS++KRQ
Sbjct: 1   MSDLQTPLVRPKRKKTWVDYFVKFRWIIVIFIVLPFSATFYFLIYLGDMWSESKSFEKRQ 60

Query: 61  KEHDENVKKVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILD 120
           KEHDENVKKV+KRLK R+ +KDGLVCTARKPWIAVGMRNVDYKRARHFEVDL  FRNIL+
Sbjct: 61  KEHDENVKKVIKRLKGRDASKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLGEFRNILE 120

Query: 121 IDKDRMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLF 180
           I+K++M A+VEPLVNMGQISRATVP+NLSLAVVAELDDLTVGGLINGYGIEGSSH+YGLF
Sbjct: 121 INKEKMTARVEPLVNMGQISRATVPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLF 180

Query: 181 SDTVVAYEIVLADGQVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTY 240
           +DTV AYEIVLA G++VRAT+DNEYSDL+YAIPWSQGT+GLLVAAEI+LI +KEYM++TY
Sbjct: 181 ADTVEAYEIVLAGGELVRATRDNEYSDLYYAIPWSQGTLGLLVAAEIRLIKVKEYMRLTY 240

Query: 241 KPFRGTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKEEAKK 300
            P +G L+ + Q Y+DS AP+DGD+    K+PDFVE M+Y P+E V+M G YASKEEAKK
Sbjct: 241 IPVKGDLQALAQGYIDSFAPKDGDK---SKIPDFVEGMVYNPTEGVMMVGTYASKEEAKK 300

Query: 301 RGNVINQIGWWFKPWFYQHASTALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPFADQW 360
           +GN IN +GWWFKPWFYQHA TALKKG+FVEYIPTR+YYHRHTR LYWEGKLILPF DQ+
Sbjct: 301 KGNKINNVGWWFKPWFYQHAQTALKKGQFVEYIPTREYYHRHTRCLYWEGKLILPFGDQF 360

Query: 361 WFRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHREFEVYP 420
           WFR+LLGW+MPPKVSLLKATQGEAIRNYYH+MHVIQDMLVPLYKV DALEW HRE EVYP
Sbjct: 361 WFRYLLGWLMPPKVSLLKATQGEAIRNYYHDMHVIQDMLVPLYKVGDALEWVHREMEVYP 420

Query: 421 IWLCPHRIFKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGLYYAPGPVLRGEVFDGAE 480
           IWLCPH++FK P+K  IYPEPGFE   RQGDT  AQMYTDVG+YYAPG VLRGE FDG+E
Sbjct: 421 IWLCPHKLFKQPIKGQIYPEPGFEYENRQGDTEDAQMYTDVGVYYAPGCVLRGEEFDGSE 480

Query: 481 AVRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAELYEQCRKKYGAVGTFMTVYYKSKK 540
           AVRRMEKWL+ENHGFQ  YAVSEL+EK FWRMF+ ELYE+CRKKY A+GTFM+VYYKSKK
Sbjct: 481 AVRRMEKWLIENHGFQPQYAVSELDEKSFWRMFNGELYEECRKKYRAIGTFMSVYYKSKK 540

Query: 541 GRKTEKEVREAEQAHLETTYAELE 564
           GRKTEKEVREAEQAHLET YAE +
Sbjct: 541 GRKTEKEVREAEQAHLETAYAEAD 561

BLAST of CaUC09G162250 vs. TAIR 10
Match: AT3G19820.2 (cell elongation protein / DWARF1 / DIMINUTO (DIM) )

HSP 1 Score: 947.2 bits (2447), Expect = 6.0e-276
Identity = 446/564 (79.08%), Postives = 504/564 (89.36%), Query Frame = 0

Query: 1   MSDLEVPL-RPKRKKVWVDYFVQFRWILVIFVVLPISFSLYFFTYLGDVRSAWKSYKKRQ 60
           MSDL+ PL RPKRKK WVDYFV+FRWI+VIF+VLP S + YF  YLGD+ S  KS++KRQ
Sbjct: 1   MSDLQTPLVRPKRKKTWVDYFVKFRWIIVIFIVLPFSATFYFLIYLGDMWSESKSFEKRQ 60

Query: 61  KEHDENVKKVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILD 120
           KEHDENVKKV+KRLK R+ +KDGLVCTARKPWIAVGMRNVDYKRARHFEVDL  FRNIL+
Sbjct: 61  KEHDENVKKVIKRLKGRDASKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLGEFRNILE 120

Query: 121 IDKDRMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLF 180
           I+K++M A+VEPLVNMGQISRATVP+NLSLAVVAELDDLTVGGLINGYGIEGSSH+YGLF
Sbjct: 121 INKEKMTARVEPLVNMGQISRATVPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLF 180

Query: 181 SDTVVAYEIVLADGQVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTY 240
           +DTV AYEIVLA G++VRAT+DNEYSDL+YAIPWSQGT+GLLVAAEI+LI +KEYM++TY
Sbjct: 181 ADTVEAYEIVLAGGELVRATRDNEYSDLYYAIPWSQGTLGLLVAAEIRLIKVKEYMRLTY 240

Query: 241 KPFRGTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKEEAKK 300
            P +G L+ + Q Y+DS AP+DGD+    K+PDFVE M+Y P+E V+M G YASKEEAKK
Sbjct: 241 IPVKGDLQALAQGYIDSFAPKDGDK---SKIPDFVEGMVYNPTEGVMMVGTYASKEEAKK 300

Query: 301 RGNVINQIGWWFKPWFYQHASTALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPFADQW 360
           +GN IN +GWWFKPWFYQHA TALKKG+FVEYIPTR+YYHRHTR LYWEGKLILPF DQ+
Sbjct: 301 KGNKINNVGWWFKPWFYQHAQTALKKGQFVEYIPTREYYHRHTRCLYWEGKLILPFGDQF 360

Query: 361 WFRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHREFEVYP 420
           WFR+LLGW+MPPKVSLLKATQGEAIRNYYH+MHVIQDMLVPLYKV DALEW HRE EVYP
Sbjct: 361 WFRYLLGWLMPPKVSLLKATQGEAIRNYYHDMHVIQDMLVPLYKVGDALEWVHREMEVYP 420

Query: 421 IWLCPHRIFKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGLYYAPGPVLRGEVFDGAE 480
           IWLCPH++FK P+K  IYPEPGFE   RQGDT  AQMYTDVG+YYAPG VLRGE FDG+E
Sbjct: 421 IWLCPHKLFKQPIKGQIYPEPGFEYENRQGDTEDAQMYTDVGVYYAPGCVLRGEEFDGSE 480

Query: 481 AVRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAELYEQCRKKYGAVGTFMTVYYKSKK 540
           AVRRMEKWL+ENHGFQ  YAVSEL+EK FWRMF+ ELYE+CRKKY A+GTFM+VYYKSKK
Sbjct: 481 AVRRMEKWLIENHGFQPQYAVSELDEKSFWRMFNGELYEECRKKYRAIGTFMSVYYKSKK 540

Query: 541 GRKTEKEVREAEQAHLETTYAELE 564
           GRKTEKEVREAEQAHLET YAE +
Sbjct: 541 GRKTEKEVREAEQAHLETAYAEAD 561

BLAST of CaUC09G162250 vs. TAIR 10
Match: AT3G19820.3 (cell elongation protein / DWARF1 / DIMINUTO (DIM) )

HSP 1 Score: 947.2 bits (2447), Expect = 6.0e-276
Identity = 446/564 (79.08%), Postives = 504/564 (89.36%), Query Frame = 0

Query: 1   MSDLEVPL-RPKRKKVWVDYFVQFRWILVIFVVLPISFSLYFFTYLGDVRSAWKSYKKRQ 60
           MSDL+ PL RPKRKK WVDYFV+FRWI+VIF+VLP S + YF  YLGD+ S  KS++KRQ
Sbjct: 1   MSDLQTPLVRPKRKKTWVDYFVKFRWIIVIFIVLPFSATFYFLIYLGDMWSESKSFEKRQ 60

Query: 61  KEHDENVKKVVKRLKQRNPAKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILD 120
           KEHDENVKKV+KRLK R+ +KDGLVCTARKPWIAVGMRNVDYKRARHFEVDL  FRNIL+
Sbjct: 61  KEHDENVKKVIKRLKGRDASKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLGEFRNILE 120

Query: 121 IDKDRMIAKVEPLVNMGQISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLF 180
           I+K++M A+VEPLVNMGQISRATVP+NLSLAVVAELDDLTVGGLINGYGIEGSSH+YGLF
Sbjct: 121 INKEKMTARVEPLVNMGQISRATVPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLF 180

Query: 181 SDTVVAYEIVLADGQVVRATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIPIKEYMKVTY 240
           +DTV AYEIVLA G++VRAT+DNEYSDL+YAIPWSQGT+GLLVAAEI+LI +KEYM++TY
Sbjct: 181 ADTVEAYEIVLAGGELVRATRDNEYSDLYYAIPWSQGTLGLLVAAEIRLIKVKEYMRLTY 240

Query: 241 KPFRGTLREIGQAYMDSLAPRDGDQDNPEKVPDFVETMIYTPSEAVVMTGRYASKEEAKK 300
            P +G L+ + Q Y+DS AP+DGD+    K+PDFVE M+Y P+E V+M G YASKEEAKK
Sbjct: 241 IPVKGDLQALAQGYIDSFAPKDGDK---SKIPDFVEGMVYNPTEGVMMVGTYASKEEAKK 300

Query: 301 RGNVINQIGWWFKPWFYQHASTALKKGEFVEYIPTRDYYHRHTRSLYWEGKLILPFADQW 360
           +GN IN +GWWFKPWFYQHA TALKKG+FVEYIPTR+YYHRHTR LYWEGKLILPF DQ+
Sbjct: 301 KGNKINNVGWWFKPWFYQHAQTALKKGQFVEYIPTREYYHRHTRCLYWEGKLILPFGDQF 360

Query: 361 WFRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVPDALEWAHREFEVYP 420
           WFR+LLGW+MPPKVSLLKATQGEAIRNYYH+MHVIQDMLVPLYKV DALEW HRE EVYP
Sbjct: 361 WFRYLLGWLMPPKVSLLKATQGEAIRNYYHDMHVIQDMLVPLYKVGDALEWVHREMEVYP 420

Query: 421 IWLCPHRIFKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGLYYAPGPVLRGEVFDGAE 480
           IWLCPH++FK P+K  IYPEPGFE   RQGDT  AQMYTDVG+YYAPG VLRGE FDG+E
Sbjct: 421 IWLCPHKLFKQPIKGQIYPEPGFEYENRQGDTEDAQMYTDVGVYYAPGCVLRGEEFDGSE 480

Query: 481 AVRRMEKWLLENHGFQALYAVSELNEKDFWRMFDAELYEQCRKKYGAVGTFMTVYYKSKK 540
           AVRRMEKWL+ENHGFQ  YAVSEL+EK FWRMF+ ELYE+CRKKY A+GTFM+VYYKSKK
Sbjct: 481 AVRRMEKWLIENHGFQPQYAVSELDEKSFWRMFNGELYEECRKKYRAIGTFMSVYYKSKK 540

Query: 541 GRKTEKEVREAEQAHLETTYAELE 564
           GRKTEKEVREAEQAHLET YAE +
Sbjct: 541 GRKTEKEVREAEQAHLETAYAEAD 561

BLAST of CaUC09G162250 vs. TAIR 10
Match: AT2G41510.1 (cytokinin oxidase/dehydrogenase 1 )

HSP 1 Score: 51.2 bits (121), Expect = 3.1e-06
Identity = 42/144 (29.17%), Postives = 67/144 (46.53%), Query Frame = 0

Query: 138 ISRATVPLNLSLAVVAELDDLTVGGLINGYGIEGSSHLYGLFSDTVVAYEIVLADGQVVR 197
           I R T+   LS     +   LTVGG ++  GI G +  +G   + V   EIV   G+VV 
Sbjct: 170 ILRETLKYGLSPKSWTDYLHLTVGGTLSNAGISGQAFKHGPQINNVYQLEIVTGKGEVVT 229

Query: 198 ATKDNEYSDLFYAIPWSQGTIGLLVAAEIKLIP---IKEYMKVTYKPFRGTLREIGQAYM 257
            + +   S+LF+++    G  G++  A I L P   + ++++V Y  F    R+  Q Y+
Sbjct: 230 CS-EKRNSELFFSVLGGLGQFGIITRARISLEPAPHMVKWIRVLYSDFSAFSRD--QEYL 289

Query: 258 DSLAPRDGDQDNPEKVPDFVETMI 279
            S           EK  D+VE  +
Sbjct: 290 IS----------KEKTFDYVEGFV 300

BLAST of CaUC09G162250 vs. TAIR 10
Match: AT5G56970.1 (cytokinin oxidase 3 )

HSP 1 Score: 50.8 bits (120), Expect = 4.1e-06
Identity = 41/140 (29.29%), Postives = 64/140 (45.71%), Query Frame = 0

Query: 89  KPWIAVGMRNVDYKRARHFEVDLSAFRNILDIDKDRMIAKVEPLVNMGQISRATVPLNLS 148
           K  + V MR++   R R  +V     R  L +D D     +E L         T+ L L+
Sbjct: 114 KDGVVVNMRSM-VNRDRGIKVS----RTCLYVDVDAAWLWIEVL-------NKTLELGLT 173

Query: 149 LAVVAELDDLTVGGLINGYGIEGSSHLYGLFSDTVVAYEIVLADGQVVRATKDNEYSDLF 208
                +   LTVGG ++  GI G +  YG     V+  +++   G++   +KD   SDLF
Sbjct: 174 PVSWTDYLYLTVGGTLSNGGISGQTFRYGPQITNVLEMDVITGKGEIATCSKDMN-SDLF 233

Query: 209 YAIPWSQGTIGLLVAAEIKL 229
           +A+    G  G++  A IKL
Sbjct: 234 FAVLGGLGQFGIITRARIKL 240

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038898378.10.0e+0099.12delta(24)-sterol reductase [Benincasa hispida] >XP_038898379.1 delta(24)-sterol ... [more]
KAG7014278.10.0e+0097.88Delta(24)-sterol reductase [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023548025.10.0e+0097.70delta(24)-sterol reductase [Cucurbita pepo subsp. pepo] >XP_023548027.1 delta(24... [more]
XP_022953594.10.0e+0097.70delta(24)-sterol reductase [Cucurbita moschata] >XP_022953595.1 delta(24)-sterol... [more]
XP_022960164.10.0e+0097.00delta(24)-sterol reductase-like [Cucurbita moschata] >XP_022960165.1 delta(24)-s... [more]
Match NameE-valueIdentityDescription
P934728.2e-29484.25Delta(24)-sterol reductase OS=Pisum sativum OX=3888 GN=DIM PE=1 SV=1[more]
Q390858.5e-27579.08Delta(24)-sterol reductase OS=Arabidopsis thaliana OX=3702 GN=DIM PE=1 SV=2[more]
Q153927.5e-10641.52Delta(24)-sterol reductase OS=Homo sapiens OX=9606 GN=DHCR24 PE=1 SV=2[more]
Q8VCH62.2e-10541.52Delta(24)-sterol reductase OS=Mus musculus OX=10090 GN=Dhcr24 PE=1 SV=1[more]
Q60HC52.9e-10541.33Delta(24)-sterol reductase OS=Macaca fascicularis OX=9541 GN=DHCR24 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1GQ500.0e+0097.70Delta(24)-sterol reductase OS=Cucurbita moschata OX=3662 GN=LOC111456086 PE=4 SV... [more]
A0A6J1H6V40.0e+0097.00Delta(24)-sterol reductase OS=Cucurbita moschata OX=3662 GN=LOC111460987 PE=4 SV... [more]
A0A6J1JUW60.0e+0097.35Delta(24)-sterol reductase OS=Cucurbita maxima OX=3661 GN=LOC111488060 PE=4 SV=1[more]
A0A6J1KZQ60.0e+0096.82Delta(24)-sterol reductase OS=Cucurbita maxima OX=3661 GN=LOC111497787 PE=4 SV=1[more]
A0A1S3CE880.0e+0095.94Delta(24)-sterol reductase OS=Cucumis melo OX=3656 GN=LOC103499976 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT3G19820.16.0e-27679.08cell elongation protein / DWARF1 / DIMINUTO (DIM) [more]
AT3G19820.26.0e-27679.08cell elongation protein / DWARF1 / DIMINUTO (DIM) [more]
AT3G19820.36.0e-27679.08cell elongation protein / DWARF1 / DIMINUTO (DIM) [more]
AT2G41510.13.1e-0629.17cytokinin oxidase/dehydrogenase 1 [more]
AT5G56970.14.1e-0629.29cytokinin oxidase 3 [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (USVL246-FR2) v1
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006094FAD linked oxidase, N-terminalPFAMPF01565FAD_binding_4coord: 109..198
e-value: 1.9E-14
score: 53.6
IPR016169FAD-binding, type PCMH, subdomain 2GENE3D3.30.465.10coord: 110..231
e-value: 2.9E-26
score: 93.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 541..556
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 541..566
NoneNo IPR availablePANTHERPTHR10801:SF16S-LOCUS GLYCOPROTEIN DOMAIN, BULB-TYPE LECTIN DOMAIN, FAD-BINDING, TYPE 2-RELATEDcoord: 1..564
IPR040165Delta(24)-sterol reductasePANTHERPTHR1080124-DEHYDROCHOLESTEROL REDUCTASEcoord: 1..564
IPR016166FAD-binding domain, PCMH-typePROSITEPS51387FAD_PCMHcoord: 45..231
score: 13.133762
IPR036318FAD-binding, type PCMH-like superfamilySUPERFAMILY56176FAD-binding/transporter-associated domain-likecoord: 105..231

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CaUC09G162250.1CaUC09G162250.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008202 steroid metabolic process
cellular_component GO:0005737 cytoplasm
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0050614 delta24-sterol reductase activity
molecular_function GO:0071949 FAD binding
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0016491 oxidoreductase activity