CaUC09G158960 (gene) Watermelon (USVL246-FR2) v1

Overview
NameCaUC09G158960
Typegene
OrganismCitrullus amarus (Watermelon (USVL246-FR2) v1)
DescriptionGDSL esterase/lipase
LocationCiama_Chr09: 1242276 .. 1246201 (-)
RNA-Seq ExpressionCaUC09G158960
SyntenyCaUC09G158960
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCATTATTTAACCTTGAAGGGAATAAGATGGGTCTAAACTTTTAATTTAATCAGAATACACTTTAATTTTAAATAGGTATGGCAAGTTGTCATTCGTTAAAAACTTAAAATTGGTCAGATTTGGTTGGGTTATTTAATGGTTTATGTTGGGTGTGAATTAAAATTTAATTTTATTCAAAACTTTTTTTTTGGTTAAATTATGGAGTTTTTGAATTCTTATTTGTTAAAACTGCTTATTGAGAAATAGCATCTACAAATAATGTGTTTGTAAGTCCACTCTACTTTATACGAATCTTCTCCATCGATCAGTTTCAAAGGACAAAGATATGAAGATATCAAACTTCCATTTCCTTTTCTTGATTTTCTCCGCATTGTTCTTCATCGCACAAAGCTCTCAAATCAACGATGTTCCTTCATCGCAAAAACGCCTCGCCTTCTTCATATTCGGCGATTCCTTTTTCGATCCTGGAAACAACAATTTCATCAACACCACCCAGGATTTCCGCGCCAATTTCACGCCCTACGGTGAAACTTTCTTCAAATATCCGACCGGAAGATTCTCCGACGGCCGTCTCATCCCAGATTTCATCGGTAATTACTCAAAATAGAGACTGAAACTAAAATTCCTACTCGTTTCAATTTCGTCTCTCCTTGTTCTTACAAGAAAATTGTTCTTACAGCCGAGTATGCGAATTTGCCCTTGATTCCGGCGTATTTAGATCCTCGCAACAAACTCTACATCCATGGAGTTAATTTTGCATCCGGTGGAGGCGGCGCTTTGGTCGAAACTCACCGAGGATTTGTACCATTCTCCAATCGATCAAACCGCATTGTTTTGCCGTTTTCGCGATCTTTCTTTTTCTATGCTGAAAATTGACTGTGTTGATTTAATTTTCAGGCGATAGACATTGAAACTCAACTTAGGTATTTCATGAAGGTGGAGAGATCGGTGAGGAAGAAACTGGGAGATTCGAGAGCGTATAGCTTGTTTTCGAACTCTGTTTATATGTTCAGTATTGGCGGAAACGATTACATCGTTCCGTTTGAAGGTTCCACCGTTCTTGAGAAGTACACTGAAACAGAGTATGTGAATATGGTGCTCGGAAACGCCACGGCGGTGCTCGAAGTAAGTTTCAATCTCTCGAAGCTTAAATTTAACGTGTTTATTCGGTGAATTCAACTTTGAAAATTGCATTTTTTTTAAAAAAAATAGAAAATTACATTTTTGTCCACATATAGTCACTATTTGTTTGACCTAATTTTCAAAATGGCAAGGACTTCTCATTGAGCAGGCTCAATGCTATCTTGAACAGTCCAAGAGAACGAAAACTAAGTTCAAGTTTTAAGTTTAGTTTCAATATACTTCATAGATTTCAAAATGAAATTTGAGTTTTATTTTAATTTGGACTTTTAAGTTTCAAGGTTTTACATGTTTAGTTTTGAATTTTCATTAAATACTCACTTTCTATTATTGGTATCAATGTCCATTCATTAAGTGAGATTTTAAATTTAATTTTAAAATAGATGAAAAATAATAAAATTTAATTAATTATAACTCTTTTAATTATTTTAGATTTATTAACATATATTAATACTAAAGAAGAAAAATGACTATTTAGTGAAAAAAAAAAAAGTTAAAAATATAAGGCTTGAAATCTAATAACCAAATTGAAACAAAACTCGAATGCTGAAAAAATTGTAACGTTTGGAAACTTAGAGACTAAATTAAAACAAAACTCAAATGTTAAAATTGAAAATAATGTATCTATCAAGTATAGAATTTAAAATTTAAAGTTTATTTAACATAAAATTCAATTTATTCACTGTGTTTAATTTGATGAATAATTTTTTTTTTTTAATTTCAATCTGGTCCCAATAATGCATTTGAAAATATGAAAATCAAACATGTTTATATAATTTATTTCAAGCTTTACTTTAATTTATGTCTCATTTAGCAACTATTTGGTTTTTTAAAATTATGCCTACAAATGCTTTTTCACCTTTAAATTTCATGTTTTGTTATCTACTTTTTATCAAAGTTTTAAAAAATTAAGCCAAGTTTTCAAAAAAAAAAAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAAAATTAAGCCAATTTTTTAAAAAAAAAAAAAAAAAAAAAGTAGTTTTTAGTTTAGAATTTTGATCAGAATTCAAGTTTTGTACTTATAAAAGATGTAAAAGCATGGTACAGAAAGTGAGAGGAAATCGAGTTAATTTTAAAAACTAAAAACCAAAATCAAATGGCTATGAAATTGAAATAATGTTTACAAAATCTATTTTTAGTTGGGAAAAAAATGTGTTGGTAATTTTAAAATTTGGCTAAGAGTATAAAATTTACCCTAAAAAGATAAGACAACCCTAAAAAAGATGGTAAGAAAACAATCACAATTTTCAAAAGAAAAAATTAAATAGTGAATAATTATTAAATGAGACTATAGTTTCTAATATATATTCTTTGGGTTTGGAGAATATATATCTTAACTAAAAGATGTAGTAAAAAAAAAAAAAAAAAAAGGGACAAGTAAAACACGATTTTAACCAAACCAAATAAATGATAAAAACAAACTTTCAAACCATAGGAATCTAAAGTAAATTGAAAGCGAACTGAATTATGAAAAGAAAAAAAAAGAATAGAAATTAGTTGAGAAAGGAGAAGTTTTAGAATTGAAAATGCAGTTTGATTTGGAGTAATTAATGGGATAGGAAATATACAAAAAAGGAGGAAGGAAATTTGCATTTGTGGCAGTGCCTCCATTAGGTTGTTTGCCACACAGTAGATTGATGAAAGAAGGCAGCCATGGAAGCTGTTATGATGAAGCTTCAGCTCTTGCAAGGCTTCACAATAAGCTTCTTCCTGCTGCTTTGCACAAACTTGCCAACAAACTCCAAGGATTCAAATACACCTTTGCAGATACCTATACTCTGCTTCAAAACAGAATTGACAACCCTTCAAAATATGGTAATCCCCATTTCTTAATTAATCCATCCATGTTTATGCTTTGTTCCATAAAGCTTTATTTTAATTTTTTTGCTAAGCTAGAACTTGGATGATACATATCTATTAACATTTAAACTTTCTTTTGATCTAAACACATCTCACTCAAAATTAGTCTAAGTATATAATTTGGAGGAAATAATTAAAAGTTTGTTTTGAAGATAAATCATGAGGGCCTCTACATGTAAAACCAAACATGGAAAGAATTAACTTAGGAAGAAATTAGTTGTTTAAGCTTATTTGAAAATTTAGGGGATGTTTGTTTTAGAGTAATTAAAGTGAATTATTTTGAAATAGTGTTTATGAGATTATAATGAGAGAAATGATGATGATGATGTTTGTGGATGAAGGTTTCAAGGAAGGAAAAAAAGCATGCTGTGGAAGTGGAGAGTTGAGAGGAATATACACATGTGGAGGAATGAGAGGACAGAAAGAATTTGAGTTATGTGAAAACCCCAATGAATATTTGTTTTTTGATTCTTATCATCCCAATCAAAGAGCTTATGAACAATTTGCAAAGCTGATGTGGAGTGGAGATGCCCAAGTCATCAAGCCTTACAACCTCAAACAACTCTTTCAATATGGATCATCACAACCTAATTGAACATTCACTGTATTAACTACCTTTCTATTTTCCATATATATCAATCACTCGATTACATAAACCCACATACACAACTATCACTTTATCATATATATATCTATAGATATCTACTTGGATCTATTGCGGTCTATCACAAATACGAAGTAAAATTTTGCTAT

mRNA sequence

CCATTATTTAACCTTGAAGGGAATAAGATGGGTCTAAACTTTTAATTTAATCAGAATACACTTTAATTTTAAATAGGTATGGCAAGTTGTCATTCGTTAAAAACTTAAAATTGGTCAGATTTGGTTGGGTTATTTAATGGTTTATGTTGGGTGTGAATTAAAATTTAATTTTATTCAAAACTTTTTTTTTGGTTAAATTATGGAGTTTTTGAATTCTTATTTGTTAAAACTGCTTATTGAGAAATAGCATCTACAAATAATGTGTTTGTAAGTCCACTCTACTTTATACGAATCTTCTCCATCGATCAGTTTCAAAGGACAAAGATATGAAGATATCAAACTTCCATTTCCTTTTCTTGATTTTCTCCGCATTGTTCTTCATCGCACAAAGCTCTCAAATCAACGATGTTCCTTCATCGCAAAAACGCCTCGCCTTCTTCATATTCGGCGATTCCTTTTTCGATCCTGGAAACAACAATTTCATCAACACCACCCAGGATTTCCGCGCCAATTTCACGCCCTACGGTGAAACTTTCTTCAAATATCCGACCGGAAGATTCTCCGACGGCCGTCTCATCCCAGATTTCATCGCCGAGTATGCGAATTTGCCCTTGATTCCGGCGTATTTAGATCCTCGCAACAAACTCTACATCCATGGAGTTAATTTTGCATCCGGTGGAGGCGGCGCTTTGGTCGAAACTCACCGAGGATTTGCGATAGACATTGAAACTCAACTTAGGTATTTCATGAAGGTGGAGAGATCGGTGAGGAAGAAACTGGGAGATTCGAGAGCGTATAGCTTGTTTTCGAACTCTGTTTATATGTTCAGTATTGGCGGAAACGATTACATCGTTCCGTTTGAAGGTTCCACCGTTCTTGAGAAGTACACTGAAACAGAGTATGTGAATATGGTGCTCGGAAACGCCACGGCGGTGCTCGAAGAAATATACAAAAAAGGAGGAAGGAAATTTGCATTTGTGGCAGTGCCTCCATTAGGTTGTTTGCCACACAGTAGATTGATGAAAGAAGGCAGCCATGGAAGCTGTTATGATGAAGCTTCAGCTCTTGCAAGGCTTCACAATAAGCTTCTTCCTGCTGCTTTGCACAAACTTGCCAACAAACTCCAAGGATTCAAATACACCTTTGCAGATACCTATACTCTGCTTCAAAACAGAATTGACAACCCTTCAAAATATGGTAATCCCCATTTCTTAATTAATCCATCCATGGGATGTTTCAAGGAAGGAAAAAAAGCATGCTGTGGAAGTGGAGAGTTGAGAGGAATATACACATGTGGAGGAATGAGAGGACAGAAAGAATTTGAGTTATGTGAAAACCCCAATGAATATTTGTTTTTTGATTCTTATCATCCCAATCAAAGAGCTTATGAACAATTTGCAAAGCTGATGTGGAGTGGAGATGCCCAAGTCATCAAGCCTTACAACCTCAAACAACTCTTTCAATATGGATCATCACAACCTAATTGAACATTCACTGTATTAACTACCTTTCTATTTTCCATATATATCAATCACTCGATTACATAAACCCACATACACAACTATCACTTTATCATATATATATCTATAGATATCTACTTGGATCTATTGCGGTCTATCACAAATACGAAGTAAAATTTTGCTAT

Coding sequence (CDS)

ATGAAGATATCAAACTTCCATTTCCTTTTCTTGATTTTCTCCGCATTGTTCTTCATCGCACAAAGCTCTCAAATCAACGATGTTCCTTCATCGCAAAAACGCCTCGCCTTCTTCATATTCGGCGATTCCTTTTTCGATCCTGGAAACAACAATTTCATCAACACCACCCAGGATTTCCGCGCCAATTTCACGCCCTACGGTGAAACTTTCTTCAAATATCCGACCGGAAGATTCTCCGACGGCCGTCTCATCCCAGATTTCATCGCCGAGTATGCGAATTTGCCCTTGATTCCGGCGTATTTAGATCCTCGCAACAAACTCTACATCCATGGAGTTAATTTTGCATCCGGTGGAGGCGGCGCTTTGGTCGAAACTCACCGAGGATTTGCGATAGACATTGAAACTCAACTTAGGTATTTCATGAAGGTGGAGAGATCGGTGAGGAAGAAACTGGGAGATTCGAGAGCGTATAGCTTGTTTTCGAACTCTGTTTATATGTTCAGTATTGGCGGAAACGATTACATCGTTCCGTTTGAAGGTTCCACCGTTCTTGAGAAGTACACTGAAACAGAGTATGTGAATATGGTGCTCGGAAACGCCACGGCGGTGCTCGAAGAAATATACAAAAAAGGAGGAAGGAAATTTGCATTTGTGGCAGTGCCTCCATTAGGTTGTTTGCCACACAGTAGATTGATGAAAGAAGGCAGCCATGGAAGCTGTTATGATGAAGCTTCAGCTCTTGCAAGGCTTCACAATAAGCTTCTTCCTGCTGCTTTGCACAAACTTGCCAACAAACTCCAAGGATTCAAATACACCTTTGCAGATACCTATACTCTGCTTCAAAACAGAATTGACAACCCTTCAAAATATGGTAATCCCCATTTCTTAATTAATCCATCCATGGGATGTTTCAAGGAAGGAAAAAAAGCATGCTGTGGAAGTGGAGAGTTGAGAGGAATATACACATGTGGAGGAATGAGAGGACAGAAAGAATTTGAGTTATGTGAAAACCCCAATGAATATTTGTTTTTTGATTCTTATCATCCCAATCAAAGAGCTTATGAACAATTTGCAAAGCTGATGTGGAGTGGAGATGCCCAAGTCATCAAGCCTTACAACCTCAAACAACTCTTTCAATATGGATCATCACAACCTAATTGA

Protein sequence

MKISNFHFLFLIFSALFFIAQSSQINDVPSSQKRLAFFIFGDSFFDPGNNNFINTTQDFRANFTPYGETFFKYPTGRFSDGRLIPDFIAEYANLPLIPAYLDPRNKLYIHGVNFASGGGGALVETHRGFAIDIETQLRYFMKVERSVRKKLGDSRAYSLFSNSVYMFSIGGNDYIVPFEGSTVLEKYTETEYVNMVLGNATAVLEEIYKKGGRKFAFVAVPPLGCLPHSRLMKEGSHGSCYDEASALARLHNKLLPAALHKLANKLQGFKYTFADTYTLLQNRIDNPSKYGNPHFLINPSMGCFKEGKKACCGSGELRGIYTCGGMRGQKEFELCENPNEYLFFDSYHPNQRAYEQFAKLMWSGDAQVIKPYNLKQLFQYGSSQPN
Homology
BLAST of CaUC09G158960 vs. NCBI nr
Match: XP_038897092.1 (GDSL lipase-like [Benincasa hispida])

HSP 1 Score: 694.9 bits (1792), Expect = 4.0e-196
Identity = 338/384 (88.02%), Postives = 354/384 (92.19%), Query Frame = 0

Query: 1   MKISNFHFLFLIFSALFFIAQSSQINDVPSSQKRLAFFIFGDSFFDPGNNNFINTTQDFR 60
           MKISNFHF FLIFS+LF IA+SS+I+DVP S+ RLAFFIFGDSFFDPGNNNFINTT+DFR
Sbjct: 1   MKISNFHFHFLIFSSLFLIARSSRIDDVPPSENRLAFFIFGDSFFDPGNNNFINTTEDFR 60

Query: 61  ANFTPYGETFFKYPTGRFSDGRLIPDFIAEYANLPLIPAYLDPRNKLYIHGVNFASGGGG 120
           ANFTPYGE+FFK PTGRFSDGRLIPDFIAEYANLPLIPAYLDPRNKLYIHGVNFASGGGG
Sbjct: 61  ANFTPYGESFFKCPTGRFSDGRLIPDFIAEYANLPLIPAYLDPRNKLYIHGVNFASGGGG 120

Query: 121 ALVETHRGFAIDIETQLRYFMKVERSVRKKLGDSRAYSLFSNSVYMFSIGGNDYIVPFEG 180
           ALVETHRGFAIDIETQLRYF KVERS+RKKLGDSRAY+LF NSVYMFSIGGNDYIVPFEG
Sbjct: 121 ALVETHRGFAIDIETQLRYFKKVERSMRKKLGDSRAYNLFLNSVYMFSIGGNDYIVPFEG 180

Query: 181 STVLEKYTETEYVNMVLGNATAVLEEIYKKGGRKFAFVAVPPLGCLPHSRLMKEGSHGSC 240
           S VLEKYTETEYVNMV+GN TAVLEEIYKKGGRKFAF AVPPLGCLPH RLMK+G HGSC
Sbjct: 181 SPVLEKYTETEYVNMVIGNTTAVLEEIYKKGGRKFAFAAVPPLGCLPHIRLMKKGGHGSC 240

Query: 241 YDEASALARLHNKLLPAALHKLANKLQGFKYTFADTYTLLQNRIDNPSKYGNPHFLINPS 300
           +DEASAL RLHNKLLP AL KLA+KLQGFKYT ADTYTLLQNRIDNPSKYG         
Sbjct: 241 WDEASALTRLHNKLLPIALQKLADKLQGFKYTVADTYTLLQNRIDNPSKYG--------- 300

Query: 301 MGCFKEGKKACCGSGELRGIYTCGGMRGQKEFELCENPNEYLFFDSYHPNQRAYEQFAKL 360
              FKEGKKACCGSGELRGIY+CGGMRGQKEFELCENPNEYLFFDSYHPN++AYEQFAKL
Sbjct: 301 ---FKEGKKACCGSGELRGIYSCGGMRGQKEFELCENPNEYLFFDSYHPNEKAYEQFAKL 360

Query: 361 MWSGDAQVIKPYNLKQLFQYGSSQ 385
           MWSGDAQVIKPYNLKQLFQ G SQ
Sbjct: 361 MWSGDAQVIKPYNLKQLFQCGLSQ 372

BLAST of CaUC09G158960 vs. NCBI nr
Match: XP_008462253.1 (PREDICTED: GDSL esterase/lipase 5-like isoform X1 [Cucumis melo] >KAA0059365.1 GDSL esterase/lipase 5-like isoform X1 [Cucumis melo var. makuwa] >TYK03961.1 GDSL esterase/lipase 5-like isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 673.7 bits (1737), Expect = 9.4e-190
Identity = 325/385 (84.42%), Postives = 347/385 (90.13%), Query Frame = 0

Query: 1   MKISNFHFLFLIFSALFFIAQSSQINDVPSSQKRLAFFIFGDSFFDPGNNNFINTTQDFR 60
           MKIS+ HF FLIFSA+FFIAQSS+I+DV SS+KRLAFFIFGDSFFDPGNNNFINTT+DFR
Sbjct: 1   MKISSVHFFFLIFSAVFFIAQSSRIDDVSSSEKRLAFFIFGDSFFDPGNNNFINTTEDFR 60

Query: 61  ANFTPYGETFFKYPTGRFSDGRLIPDFIAEYANLPLIPAYLDPRNKLYIHGVNFASGGGG 120
           ANFTPYG++FF+ PTGRFSD RL+PDF+AEYANLPLIPAYLDPRNK YIHGVNFASGGGG
Sbjct: 61  ANFTPYGKSFFRTPTGRFSDSRLVPDFVAEYANLPLIPAYLDPRNKRYIHGVNFASGGGG 120

Query: 121 ALVETHRGFAIDIETQLRYFMKVERSVRKKLGDSRAYSLFSNSVYMFSIGGNDYIVPFEG 180
           ALVETHRGFAIDIETQLRYF KVERS+RKKLGDSRAY+LFSNSVY+FSIGGNDYIVPFEG
Sbjct: 121 ALVETHRGFAIDIETQLRYFKKVERSIRKKLGDSRAYNLFSNSVYLFSIGGNDYIVPFEG 180

Query: 181 STVLEKYTETEYVNMVLGNATAVLEEIYKKGGRKFAFVAVPPLGCLPHSRLMKEGSHGSC 240
           S V EKYTETEYVNMV+GN TAVLEEIYKKGGRKFAFVAVPPLGCLPH RLMK+  HGSC
Sbjct: 181 SPVFEKYTETEYVNMVIGNVTAVLEEIYKKGGRKFAFVAVPPLGCLPHIRLMKKDGHGSC 240

Query: 241 YDEASALARLHNKLLPAALHKLANKLQGFKYTFADTYTLLQNRIDNPSKYGNPHFLINPS 300
            DEASAL RLHNKLL AAL KLA+KL GFKYT  DTYT+LQNRIDNPSKYG         
Sbjct: 241 LDEASALVRLHNKLLHAALQKLADKLHGFKYTIGDTYTMLQNRIDNPSKYG--------- 300

Query: 301 MGCFKEGKKACCGSGELRGIYTCGGMRGQKEFELCENPNEYLFFDSYHPNQRAYEQFAKL 360
               KEGKKACCGSG+LRGIY+CGGMRG KEFELCENPNEYLFFDSYHPN+RAYEQFAKL
Sbjct: 301 ---LKEGKKACCGSGKLRGIYSCGGMRGVKEFELCENPNEYLFFDSYHPNERAYEQFAKL 360

Query: 361 MWSGDAQVIKPYNLKQLFQYGSSQP 386
           MWSGD+QVI PYNLKQ FQY  SQP
Sbjct: 361 MWSGDSQVINPYNLKQFFQYIQSQP 373

BLAST of CaUC09G158960 vs. NCBI nr
Match: XP_004141726.1 (GDSL lipase isoform X1 [Cucumis sativus] >KGN45451.1 hypothetical protein Csa_016520 [Cucumis sativus])

HSP 1 Score: 662.9 bits (1709), Expect = 1.7e-186
Identity = 320/386 (82.90%), Postives = 344/386 (89.12%), Query Frame = 0

Query: 1   MKISNFHFLFLIFSALFFIAQSSQINDVPSSQKRLAFFIFGDSFFDPGNNNFINTTQDFR 60
           MKI N HFLFLIF+A+FFIAQSS I+DV S +KRLAFFIFGDS FDPGNNNFINTT+DFR
Sbjct: 1   MKIPNLHFLFLIFTAVFFIAQSSLIDDVSSPEKRLAFFIFGDSLFDPGNNNFINTTEDFR 60

Query: 61  ANFTPYGETFFKYPTGRFSDGRLIPDFIAEYANLPLIPAYLDPRNKLYIHGVNFASGGGG 120
           ANFTPYGE+FFK PTGRFSDGRL+PDF+AEYANLPLIPAYLDP NK YIHGVNFASGGGG
Sbjct: 61  ANFTPYGESFFKTPTGRFSDGRLVPDFVAEYANLPLIPAYLDPHNKRYIHGVNFASGGGG 120

Query: 121 ALVETHRGFAIDIETQLRYFMKVERSVRKKLGDSRAYSLFSNSVYMFSIGGNDYIVPFEG 180
           ALVETHRGFAIDIETQLRYF KVERS+RKKLGD RAY+LFSNSVY+FSIGGNDYIVPFEG
Sbjct: 121 ALVETHRGFAIDIETQLRYFKKVERSIRKKLGDWRAYNLFSNSVYLFSIGGNDYIVPFEG 180

Query: 181 STVLEKYTETEYVNMVLGNATAVLEEIYKKGGRKFAFVAVPPLGCLPHSRLMKE-GSHGS 240
           S + +KYTE EYVNMV+GNATAVLEEIYKKGGRKFAFVAVPPLGCLPH RL+K+ G HGS
Sbjct: 181 SPIFDKYTEREYVNMVIGNATAVLEEIYKKGGRKFAFVAVPPLGCLPHIRLVKKAGGHGS 240

Query: 241 CYDEASALARLHNKLLPAALHKLANKLQGFKYTFADTYTLLQNRIDNPSKYGNPHFLINP 300
           C+DE SAL RLHNKLLP AL KLA+KLQGFKYT  DTYT+LQNRIDNPSKYG        
Sbjct: 241 CWDEPSALVRLHNKLLPGALQKLADKLQGFKYTVGDTYTMLQNRIDNPSKYG-------- 300

Query: 301 SMGCFKEGKKACCGSGELRGIYTCGGMRGQKEFELCENPNEYLFFDSYHPNQRAYEQFAK 360
               FKE K ACCGSG+ RGIY+CGGMRG KEFELCENPNEYLFFDSYHPN+RAYEQFAK
Sbjct: 301 ----FKEEKTACCGSGKFRGIYSCGGMRGVKEFELCENPNEYLFFDSYHPNERAYEQFAK 360

Query: 361 LMWSGDAQVIKPYNLKQLFQYGSSQP 386
           LMWSGD+QVI PY+LKQ FQY SSQP
Sbjct: 361 LMWSGDSQVINPYSLKQFFQYASSQP 374

BLAST of CaUC09G158960 vs. NCBI nr
Match: XP_022964041.1 (GDSL esterase/lipase 5-like [Cucurbita moschata])

HSP 1 Score: 637.9 bits (1644), Expect = 5.7e-179
Identity = 308/384 (80.21%), Postives = 333/384 (86.72%), Query Frame = 0

Query: 1   MKISNFHFLFLIFSALFFIAQSSQINDVPSSQKRLAFFIFGDSFFDPGNNNFINTTQDFR 60
           MKISN HF F IF  + F +  SQI+DVPSS   +AFF+FGDSF DPGNNNFINTT+DFR
Sbjct: 5   MKISNVHFFFKIFFTILFTSHGSQIDDVPSSNNGVAFFVFGDSFLDPGNNNFINTTEDFR 64

Query: 61  ANFTPYGETFFKYPTGRFSDGRLIPDFIAEYANLPLIPAYLDPRNKLYIHGVNFASGGGG 120
           ANFTPYGETFF  PTGRFSDGRLIPDFIAEYANLPLIPAYLDP N LYIHGVNFASGGGG
Sbjct: 65  ANFTPYGETFFNSPTGRFSDGRLIPDFIAEYANLPLIPAYLDPHNNLYIHGVNFASGGGG 124

Query: 121 ALVETHRGFAIDIETQLRYFMKVERSVRKKLGDSRAYSLFSNSVYMFSIGGNDYIVPFEG 180
           ALVETH+GFAI+IETQLRYF KVERS+RKKLGD+RA SL S+SVYMFSIGGNDYIV FEG
Sbjct: 125 ALVETHQGFAINIETQLRYFKKVERSLRKKLGDARASSLLSDSVYMFSIGGNDYIVAFEG 184

Query: 181 STVLEKYTETEYVNMVLGNATAVLEEIYKKGGRKFAFVAVPPLGCLPHSRLMKEGSHGSC 240
           S VLEKYTE EYVNMV+GN T+VL+EIYKKGGRKFAF+AVPPLGC+PH+RLMK G HGSC
Sbjct: 185 SPVLEKYTEIEYVNMVIGNVTSVLQEIYKKGGRKFAFLAVPPLGCMPHTRLMKVGGHGSC 244

Query: 241 YDEASALARLHNKLLPAALHKLANKLQGFKYTFADTYTLLQNRIDNPSKYGNPHFLINPS 300
           +DEASALARLHNKLLPAAL KLA+ LQGFKYT ADTY +LQNRIDNPSKYG         
Sbjct: 245 WDEASALARLHNKLLPAALQKLAHDLQGFKYTLADTYNMLQNRIDNPSKYG--------- 304

Query: 301 MGCFKEGKKACCGSGELRGIYTCGGMRGQKEFELCENPNEYLFFDSYHPNQRAYEQFAKL 360
               K+G+ ACCGSGELRGIY+CGGMRGQ EFELCENPNEYLFFDSYHPN+RAYEQFAKL
Sbjct: 305 ---LKDGRTACCGSGELRGIYSCGGMRGQTEFELCENPNEYLFFDSYHPNERAYEQFAKL 364

Query: 361 MWSGDAQVIKPYNLKQLFQYGSSQ 385
           +W GDAQ I P NLKQ FQ GSSQ
Sbjct: 365 IWYGDAQFINPSNLKQFFQCGSSQ 376

BLAST of CaUC09G158960 vs. NCBI nr
Match: XP_023515160.1 (GDSL esterase/lipase 5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 636.0 bits (1639), Expect = 2.2e-178
Identity = 305/384 (79.43%), Postives = 333/384 (86.72%), Query Frame = 0

Query: 1   MKISNFHFLFLIFSALFFIAQSSQINDVPSSQKRLAFFIFGDSFFDPGNNNFINTTQDFR 60
           MKISN HF F IF  + F +  SQI D+PSS   +AFF+FGDS  DPGNNNFINTT+DFR
Sbjct: 5   MKISNVHFFFKIFFTILFTSHGSQIGDIPSSNNGVAFFVFGDSLLDPGNNNFINTTEDFR 64

Query: 61  ANFTPYGETFFKYPTGRFSDGRLIPDFIAEYANLPLIPAYLDPRNKLYIHGVNFASGGGG 120
           ANFTPYGETFF  PTGRFSDGRLIPDFIAEYANLPLIPAYLDP N LYIHGVNFASGGGG
Sbjct: 65  ANFTPYGETFFNSPTGRFSDGRLIPDFIAEYANLPLIPAYLDPHNNLYIHGVNFASGGGG 124

Query: 121 ALVETHRGFAIDIETQLRYFMKVERSVRKKLGDSRAYSLFSNSVYMFSIGGNDYIVPFEG 180
           ALVETH+GFAI+IETQLRYF KVERS+RKKLGD+RA SL S+SVYMFSIGGNDYIV FEG
Sbjct: 125 ALVETHQGFAINIETQLRYFKKVERSLRKKLGDARASSLLSDSVYMFSIGGNDYIVAFEG 184

Query: 181 STVLEKYTETEYVNMVLGNATAVLEEIYKKGGRKFAFVAVPPLGCLPHSRLMKEGSHGSC 240
           S VLEKYTE EYVNMV+GN T+VL+EIYKKGGRKFAF+AVPPLGC+PH+RLMK G HGSC
Sbjct: 185 SPVLEKYTEIEYVNMVIGNVTSVLQEIYKKGGRKFAFLAVPPLGCMPHTRLMKVGGHGSC 244

Query: 241 YDEASALARLHNKLLPAALHKLANKLQGFKYTFADTYTLLQNRIDNPSKYGNPHFLINPS 300
           +DEASALARLHNKLLPAAL KLA+ LQGFKYT ADTY +LQ RIDNPSKYG         
Sbjct: 245 WDEASALARLHNKLLPAALQKLAHDLQGFKYTLADTYNMLQTRIDNPSKYG--------- 304

Query: 301 MGCFKEGKKACCGSGELRGIYTCGGMRGQKEFELCENPNEYLFFDSYHPNQRAYEQFAKL 360
               K+G++ACCGSGELRGIY+CGGMRGQ+EFELCENPNEYLFFDSYHPN+RAYEQFAKL
Sbjct: 305 ---LKDGRRACCGSGELRGIYSCGGMRGQREFELCENPNEYLFFDSYHPNERAYEQFAKL 364

Query: 361 MWSGDAQVIKPYNLKQLFQYGSSQ 385
           +W+GDAQ I P NLKQ FQ GSSQ
Sbjct: 365 IWNGDAQFINPSNLKQFFQCGSSQ 376

BLAST of CaUC09G158960 vs. ExPASy Swiss-Prot
Match: H6U1I8 (GDSL lipase OS=Tanacetum cinerariifolium OX=118510 GN=GLIP PE=1 SV=1)

HSP 1 Score: 364.8 bits (935), Expect = 1.2e-99
Identity = 181/350 (51.71%), Postives = 233/350 (66.57%), Query Frame = 0

Query: 30  SSQKRLAFFIFGDSFFDPGNNNFINTTQDFRANFTPYGETFFKYPTGRFSDGRLIPDFIA 89
           SSQ+  A FIFGDS FDPGNNN INT  +F+ANF PYG+++F  PTGRFSDGR+IPDFIA
Sbjct: 27  SSQQAAALFIFGDSVFDPGNNNHINTHVNFKANFWPYGQSYFSSPTGRFSDGRIIPDFIA 86

Query: 90  EYANLPLIPAYLDPRNKLYIHGVNFASGGGGALVETHRGFAIDIETQLRYFMKVERSVRK 149
           EYA+LP+IPAYL+P N  + HG NFAS G GAL+ +H G A+ ++TQLRYF  +    R+
Sbjct: 87  EYASLPIIPAYLEPNND-FTHGANFASAGAGALIASHAGLAVGLQTQLRYFGDLVDHYRQ 146

Query: 150 KLGDSRAYSLFSNSVYMFSIGGNDYIVPFEGSTVLEKYTETEYVNMVLGNATAVLEEIYK 209
            LGD ++  L S++VY+FS GGNDY  P+        YT+ +YV++V+GN T V++ IY+
Sbjct: 147 NLGDIKSRQLLSDAVYLFSCGGNDYQSPY------YPYTQEQYVDIVIGNMTNVIKGIYE 206

Query: 210 KGGRKFAFVAVPPLGCLPHSRLMKEGSHGSCYDEASALARLHNKLLPAALHKLANKLQGF 269
           KGGRKF  V VP +GC P  R  + G+  +C  E   L RLHN+     L +L  +L+GF
Sbjct: 207 KGGRKFGVVNVPLIGCWPGMRAKQPGN--TCNTEVDELTRLHNQAFAKRLEQLEKQLEGF 266

Query: 270 KYTFADTYTLLQNRIDNPSKYGNPHFLINPSMGCFKEGKKACCGSGELRGIYTCGGMRGQ 329
            Y   D  T + NR+ NPSKYG            FKEG+ ACCGSG   G Y CG +   
Sbjct: 267 VYAKFDLSTAILNRMKNPSKYG------------FKEGESACCGSGPFGGNYDCGRI--- 326

Query: 330 KEFELCENPNEYLFFDSYHPNQRAYEQFAKLMWSGDAQVIKPYNLKQLFQ 380
           KEF LC+N  EY FFD +HPN+ A  QFA++ W GD+ V +PYNLK LF+
Sbjct: 327 KEFGLCDNATEYFFFDPFHPNELASRQFAEMFWDGDSMVTQPYNLKALFE 352

BLAST of CaUC09G158960 vs. ExPASy Swiss-Prot
Match: Q9SSA7 (GDSL esterase/lipase 5 OS=Arabidopsis thaliana OX=3702 GN=GLIP5 PE=2 SV=2)

HSP 1 Score: 347.8 bits (891), Expect = 1.6e-94
Identity = 180/382 (47.12%), Postives = 246/382 (64.40%), Query Frame = 0

Query: 3   ISNFHFLFLIFSALFFIAQSSQINDVPSSQKRLAFFIFGDSFFDPGNNNFINTTQDFRAN 62
           IS+F F  +  + LF   +SS            A F+FGDSF D GNNN+INTT   +AN
Sbjct: 15  ISSFIFFIVSSTILFLAGKSSAKISHNGDNNVTALFLFGDSFLDAGNNNYINTTTLDQAN 74

Query: 63  FTPYGETFFKYPTGRFSDGRLIPDFIAEYANLPLIPAYLDPRN-KLYIHGVNFASGGGGA 122
           F PYG+TFF  PTGRFSDGRLI DFIAEYANLPLIP +L+P N +  ++GVNFAS G GA
Sbjct: 75  FPPYGQTFFGLPTGRFSDGRLISDFIAEYANLPLIPPFLEPGNSQKKLYGVNFASAGAGA 134

Query: 123 LVETHRGFAIDIETQLRYFMKVERSVRKKLGDSRAYSLFSNSVYMFSIGGNDYIVPFEGS 182
           LVET +G  I++ TQL ++ KVER  R   G   +    S +VY+ SIG NDY   F  +
Sbjct: 135 LVETFQGSVINLRTQLDHYKKVERLWRTNFGKEESKKRISRAVYLISIGSNDYSSIFLTN 194

Query: 183 TVLEKYTETEYVNMVLGNATAVLEEIYKKGGRKFAFVAVPPLGCLPHSRLMKEGSHGSCY 242
             L   + +++V++V+GN T  + EIYK GGRKF F+ VP LGC P  R+++  +  SC 
Sbjct: 195 QSL-PISMSQHVDIVIGNLTTFIHEIYKIGGRKFGFLNVPDLGCFPALRILQPKNDDSCL 254

Query: 243 DEASALARLHNKLLPAALHKLANKLQGFKYTFADTYTLLQNRIDNPSKYGNPHFLINPSM 302
            +AS LA +HN+ L   L ++  +++GFK++  D    L+ R+ +PSK+G          
Sbjct: 255 RDASRLASMHNRALTNLLFQMQRQVKGFKFSLFDMNKSLRLRMQHPSKFG---------- 314

Query: 303 GCFKEGKKACCGSGELRGIYTCGGMRGQKEFELCENPNEYLFFDSYHPNQRAYEQFAKLM 362
             FKEG++ACCG+G+ RG+++CGG R  KE++LCENP +Y+F+DS H  Q  Y QFA L+
Sbjct: 315 --FKEGEEACCGTGKWRGVFSCGGKRIVKEYQLCENPKDYIFWDSLHLTQNTYNQFANLI 374

Query: 363 WSG----DAQVIKPYNLKQLFQ 380
           W+G    D+ V+ PYN+  LFQ
Sbjct: 375 WNGGHMSDSLVVGPYNINNLFQ 383

BLAST of CaUC09G158960 vs. ExPASy Swiss-Prot
Match: Q9SYF0 (GDSL esterase/lipase 2 OS=Arabidopsis thaliana OX=3702 GN=GLIP2 PE=2 SV=1)

HSP 1 Score: 346.3 bits (887), Expect = 4.5e-94
Identity = 180/386 (46.63%), Postives = 243/386 (62.95%), Query Frame = 0

Query: 3   ISNFHFLFLIFSALFFIAQSSQINDVPSSQKRL-----AFFIFGDSFFDPGNNNFINTTQ 62
           + N     +IF A   I     IN   ++   L     A F+FGDS FD GNNN+I+T  
Sbjct: 1   MENSRSTLIIFFAYTTIILIGSINCRDNNNNNLVTNQSALFVFGDSVFDAGNNNYIDTLP 60

Query: 63  DFRANFTPYGETFFKYPTGRFSDGRLIPDFIAEYANLPLIPAYLDPRN--KLYIHGVNFA 122
            FR+N+ PYG+T FK+PTGR SDGR IPDFIAEYA LPLIPAYL P N    + +GV+FA
Sbjct: 61  SFRSNYWPYGQTTFKFPTGRVSDGRTIPDFIAEYAWLPLIPAYLQPSNGKNQFPYGVSFA 120

Query: 123 SGGGGALVETHRGFAIDIETQLRYFMKVERSVRKKLGDSRAYSLFSNSVYMFSIGGNDYI 182
           S G GALV T  G  I++++QL  F KVE+ +R  LG+++   + S +VY+F IG NDY 
Sbjct: 121 SAGAGALVGTFPGMVINLKSQLNNFKKVEKLLRSTLGEAQGKMVISRAVYLFHIGVNDYQ 180

Query: 183 VPFE-GSTVLEKYTETEYVNMVLGNATAVLEEIYKKGGRKFAFVAVPPLGCLPHSRLMKE 242
            PF   S++ +   +  YV+ V+GN TAV++E+YK GGRKF F+ +    C P S ++ +
Sbjct: 181 YPFSTNSSIFQSSPQEIYVDFVVGNTTAVIKEVYKIGGRKFGFLNMGAYDCAPASLIIDQ 240

Query: 243 GSHGSCYDEASALARLHNKLLPAALHKLANKLQGFKYTFADTYTLLQNRIDNPSKYGNPH 302
              G+C+   + L  LHN+ L + L +L  +L GFKY   D +T L  R++NPSKYG   
Sbjct: 241 TKIGTCFKPVTELINLHNEKLESGLRRLERELSGFKYALHDYHTSLSVRMNNPSKYG--- 300

Query: 303 FLINPSMGCFKEGKKACCGSGELRGIYTCGGMRG-QKEFELCENPNEYLFFDSYHPNQRA 362
                    FKEGK ACCG+G LRGI TCGG  G  + +ELCE   +YLFFD +H  ++A
Sbjct: 301 ---------FKEGKMACCGTGPLRGINTCGGRMGVSQSYELCEKVTDYLFFDHFHLTEKA 360

Query: 363 YEQFAKLMWSGDAQVIKPYNLKQLFQ 380
           ++Q A+L+WSG   V KPYNL+ LF+
Sbjct: 361 HQQIAELIWSGPTNVTKPYNLQALFE 374

BLAST of CaUC09G158960 vs. ExPASy Swiss-Prot
Match: Q9FLN0 (GDSL esterase/lipase 1 OS=Arabidopsis thaliana OX=3702 GN=GLIP1 PE=1 SV=1)

HSP 1 Score: 342.8 bits (878), Expect = 5.0e-93
Identity = 177/374 (47.33%), Postives = 233/374 (62.30%), Query Frame = 0

Query: 10  FLIFSALFFIAQSSQINDVPSSQKRLAFFIFGDSFFDPGNNNFINTTQDFRANFTPYGET 69
           FL ++ +  I   + I++      + A F+FGDS FD GNNN+I+T    R+N+ PYG+T
Sbjct: 11  FLAYTIIISIGSINCIDNNNLVTNQSALFVFGDSVFDAGNNNYIDTLSSVRSNYWPYGQT 70

Query: 70  FFKYPTGRFSDGRLIPDFIAEYANLPLIPAYLDP--RNKLYIHGVNFASGGGGALVETHR 129
            FK PTGR SDGRLIPDFIAEYA LPLIP  L P   N  + +GVNFASGG GALV T  
Sbjct: 71  TFKSPTGRVSDGRLIPDFIAEYAWLPLIPPNLQPFNGNSQFAYGVNFASGGAGALVGTFS 130

Query: 130 GFAIDIETQLRYFMKVERSVRKKLGDSRAYSLFSNSVYMFSIGGNDYIVPF-EGSTVLEK 189
           G  I++ TQL  F KVE  +R KLGD+    + S +VY+F IG NDY  PF   S++ + 
Sbjct: 131 GLVINLRTQLNNFKKVEEMLRSKLGDAEGKRVISRAVYLFHIGLNDYQYPFTTNSSLFQS 190

Query: 190 YTETEYVNMVLGNATAVLEEIYKKGGRKFAFVAVPPLGCLPHSRLMKEGSHGSCYDEASA 249
            +  +YV+ V+GN T V +E+Y  GGRKF  +   P  C P S ++ +    SC+   + 
Sbjct: 191 ISNEKYVDYVVGNMTDVFKEVYNLGGRKFGILNTGPYDCAPASLVIDQTKIRSCFQPVTE 250

Query: 250 LARLHNKLLPAALHKLANKLQGFKYTFADTYTLLQNRIDNPSKYGNPHFLINPSMGCFKE 309
           L  +HN+ L   L +L ++L GFKY   D +T L  R+++PSKYG            FKE
Sbjct: 251 LINMHNEKLLNGLRRLNHELSGFKYALHDYHTSLSERMNDPSKYG------------FKE 310

Query: 310 GKKACCGSGELRGIYTCGGMRG-QKEFELCENPNEYLFFDSYHPNQRAYEQFAKLMWSGD 369
           GKKACCGSG LRGI TCGG  G  + +ELCEN  +YLFFD +H  ++A  Q A+L+WSG 
Sbjct: 311 GKKACCGSGPLRGINTCGGRMGLSQSYELCENVTDYLFFDPFHLTEKANRQIAELIWSGP 370

Query: 370 AQVIKPYNLKQLFQ 380
             +  PYNLK LF+
Sbjct: 371 TNITGPYNLKALFE 372

BLAST of CaUC09G158960 vs. ExPASy Swiss-Prot
Match: Q9SYF5 (GDSL esterase/lipase 3 OS=Arabidopsis thaliana OX=3702 GN=GLIP3 PE=2 SV=2)

HSP 1 Score: 327.0 bits (837), Expect = 2.8e-88
Identity = 168/375 (44.80%), Postives = 229/375 (61.07%), Query Frame = 0

Query: 9   LFLIFSALFFIAQSSQINDVPSSQKRLAFFIFGDSFFDPGNNNFINTTQDFRANFTPYGE 68
           +F +++ +  I   + I++      + A F+FGDS FD GNNN+INT   FR+N  PYG+
Sbjct: 8   IFFVYTIILSIGSINCIDNNNLVTNQAALFVFGDSLFDAGNNNYINTVSSFRSNIWPYGQ 67

Query: 69  TFFKYPTGRFSDGRLIPDFIAEYANLPLIPAYLDPR--NKLYIHGVNFASGGGGALVETH 128
           T FK+PTGR SDG        E A LP IP  L P   N  + +GV+FAS G GAL E+ 
Sbjct: 68  TNFKFPTGRLSDG-------PEKAWLPSIPPNLQPNNGNNQFTYGVSFASAGAGALAESF 127

Query: 129 RGFAIDIETQLRYFMKVERSVRKKLGDSRAYSLFSNSVYMFSIGGNDYIVPFE-GSTVLE 188
            G  I++ TQL  F  VE+S+R +LGD+    +FS +VY+F IG NDY  PF   S+  +
Sbjct: 128 LGMVINLGTQLNNFKDVEKSLRSELGDAETKRVFSRAVYLFHIGANDYFYPFSANSSTFK 187

Query: 189 KYTETEYVNMVLGNATAVLEEIYKKGGRKFAFVAVPPLGCLPHSRLMKEGSHGSCYDEAS 248
             ++ ++V+ V+GN T V+EE+YK GGRKF F+ V P  C P+S +      GSC+   +
Sbjct: 188 SNSKEKFVDFVIGNITFVIEEVYKMGGRKFGFLNVGPYECSPNSLIRDRTKIGSCFKPVA 247

Query: 249 ALARLHNKLLPAALHKLANKLQGFKYTFADTYTLLQNRIDNPSKYGNPHFLINPSMGCFK 308
            L  +HNK  P  L +L  +L GF+Y   D +T L  RI++PSKYG            FK
Sbjct: 248 ELIDMHNKKFPDVLRRLQRQLSGFRYALHDYHTSLSERINSPSKYG------------FK 307

Query: 309 EGKKACCGSGELRGIYTCGGMRGQKE-FELCENPNEYLFFDSYHPNQRAYEQFAKLMWSG 368
           EGKKACCGSG LRGI TCG   G  + + LCEN  +YLF+DS H  ++A+ Q A+L+W+G
Sbjct: 308 EGKKACCGSGPLRGINTCGNRIGPSQGYGLCENVTDYLFYDSSHLTEKAHRQIAELIWNG 363

Query: 369 DAQVIKPYNLKQLFQ 380
              V +PYNLK LF+
Sbjct: 368 PPNVTRPYNLKALFE 363

BLAST of CaUC09G158960 vs. ExPASy TrEMBL
Match: A0A5A7V0K2 (GDSL esterase/lipase 5-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G001580 PE=3 SV=1)

HSP 1 Score: 673.7 bits (1737), Expect = 4.6e-190
Identity = 325/385 (84.42%), Postives = 347/385 (90.13%), Query Frame = 0

Query: 1   MKISNFHFLFLIFSALFFIAQSSQINDVPSSQKRLAFFIFGDSFFDPGNNNFINTTQDFR 60
           MKIS+ HF FLIFSA+FFIAQSS+I+DV SS+KRLAFFIFGDSFFDPGNNNFINTT+DFR
Sbjct: 1   MKISSVHFFFLIFSAVFFIAQSSRIDDVSSSEKRLAFFIFGDSFFDPGNNNFINTTEDFR 60

Query: 61  ANFTPYGETFFKYPTGRFSDGRLIPDFIAEYANLPLIPAYLDPRNKLYIHGVNFASGGGG 120
           ANFTPYG++FF+ PTGRFSD RL+PDF+AEYANLPLIPAYLDPRNK YIHGVNFASGGGG
Sbjct: 61  ANFTPYGKSFFRTPTGRFSDSRLVPDFVAEYANLPLIPAYLDPRNKRYIHGVNFASGGGG 120

Query: 121 ALVETHRGFAIDIETQLRYFMKVERSVRKKLGDSRAYSLFSNSVYMFSIGGNDYIVPFEG 180
           ALVETHRGFAIDIETQLRYF KVERS+RKKLGDSRAY+LFSNSVY+FSIGGNDYIVPFEG
Sbjct: 121 ALVETHRGFAIDIETQLRYFKKVERSIRKKLGDSRAYNLFSNSVYLFSIGGNDYIVPFEG 180

Query: 181 STVLEKYTETEYVNMVLGNATAVLEEIYKKGGRKFAFVAVPPLGCLPHSRLMKEGSHGSC 240
           S V EKYTETEYVNMV+GN TAVLEEIYKKGGRKFAFVAVPPLGCLPH RLMK+  HGSC
Sbjct: 181 SPVFEKYTETEYVNMVIGNVTAVLEEIYKKGGRKFAFVAVPPLGCLPHIRLMKKDGHGSC 240

Query: 241 YDEASALARLHNKLLPAALHKLANKLQGFKYTFADTYTLLQNRIDNPSKYGNPHFLINPS 300
            DEASAL RLHNKLL AAL KLA+KL GFKYT  DTYT+LQNRIDNPSKYG         
Sbjct: 241 LDEASALVRLHNKLLHAALQKLADKLHGFKYTIGDTYTMLQNRIDNPSKYG--------- 300

Query: 301 MGCFKEGKKACCGSGELRGIYTCGGMRGQKEFELCENPNEYLFFDSYHPNQRAYEQFAKL 360
               KEGKKACCGSG+LRGIY+CGGMRG KEFELCENPNEYLFFDSYHPN+RAYEQFAKL
Sbjct: 301 ---LKEGKKACCGSGKLRGIYSCGGMRGVKEFELCENPNEYLFFDSYHPNERAYEQFAKL 360

Query: 361 MWSGDAQVIKPYNLKQLFQYGSSQP 386
           MWSGD+QVI PYNLKQ FQY  SQP
Sbjct: 361 MWSGDSQVINPYNLKQFFQYIQSQP 373

BLAST of CaUC09G158960 vs. ExPASy TrEMBL
Match: A0A1S3CI17 (GDSL esterase/lipase 5-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103500655 PE=3 SV=1)

HSP 1 Score: 673.7 bits (1737), Expect = 4.6e-190
Identity = 325/385 (84.42%), Postives = 347/385 (90.13%), Query Frame = 0

Query: 1   MKISNFHFLFLIFSALFFIAQSSQINDVPSSQKRLAFFIFGDSFFDPGNNNFINTTQDFR 60
           MKIS+ HF FLIFSA+FFIAQSS+I+DV SS+KRLAFFIFGDSFFDPGNNNFINTT+DFR
Sbjct: 1   MKISSVHFFFLIFSAVFFIAQSSRIDDVSSSEKRLAFFIFGDSFFDPGNNNFINTTEDFR 60

Query: 61  ANFTPYGETFFKYPTGRFSDGRLIPDFIAEYANLPLIPAYLDPRNKLYIHGVNFASGGGG 120
           ANFTPYG++FF+ PTGRFSD RL+PDF+AEYANLPLIPAYLDPRNK YIHGVNFASGGGG
Sbjct: 61  ANFTPYGKSFFRTPTGRFSDSRLVPDFVAEYANLPLIPAYLDPRNKRYIHGVNFASGGGG 120

Query: 121 ALVETHRGFAIDIETQLRYFMKVERSVRKKLGDSRAYSLFSNSVYMFSIGGNDYIVPFEG 180
           ALVETHRGFAIDIETQLRYF KVERS+RKKLGDSRAY+LFSNSVY+FSIGGNDYIVPFEG
Sbjct: 121 ALVETHRGFAIDIETQLRYFKKVERSIRKKLGDSRAYNLFSNSVYLFSIGGNDYIVPFEG 180

Query: 181 STVLEKYTETEYVNMVLGNATAVLEEIYKKGGRKFAFVAVPPLGCLPHSRLMKEGSHGSC 240
           S V EKYTETEYVNMV+GN TAVLEEIYKKGGRKFAFVAVPPLGCLPH RLMK+  HGSC
Sbjct: 181 SPVFEKYTETEYVNMVIGNVTAVLEEIYKKGGRKFAFVAVPPLGCLPHIRLMKKDGHGSC 240

Query: 241 YDEASALARLHNKLLPAALHKLANKLQGFKYTFADTYTLLQNRIDNPSKYGNPHFLINPS 300
            DEASAL RLHNKLL AAL KLA+KL GFKYT  DTYT+LQNRIDNPSKYG         
Sbjct: 241 LDEASALVRLHNKLLHAALQKLADKLHGFKYTIGDTYTMLQNRIDNPSKYG--------- 300

Query: 301 MGCFKEGKKACCGSGELRGIYTCGGMRGQKEFELCENPNEYLFFDSYHPNQRAYEQFAKL 360
               KEGKKACCGSG+LRGIY+CGGMRG KEFELCENPNEYLFFDSYHPN+RAYEQFAKL
Sbjct: 301 ---LKEGKKACCGSGKLRGIYSCGGMRGVKEFELCENPNEYLFFDSYHPNERAYEQFAKL 360

Query: 361 MWSGDAQVIKPYNLKQLFQYGSSQP 386
           MWSGD+QVI PYNLKQ FQY  SQP
Sbjct: 361 MWSGDSQVINPYNLKQFFQYIQSQP 373

BLAST of CaUC09G158960 vs. ExPASy TrEMBL
Match: A0A0A0KCD4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G448130 PE=3 SV=1)

HSP 1 Score: 662.9 bits (1709), Expect = 8.1e-187
Identity = 320/386 (82.90%), Postives = 344/386 (89.12%), Query Frame = 0

Query: 1   MKISNFHFLFLIFSALFFIAQSSQINDVPSSQKRLAFFIFGDSFFDPGNNNFINTTQDFR 60
           MKI N HFLFLIF+A+FFIAQSS I+DV S +KRLAFFIFGDS FDPGNNNFINTT+DFR
Sbjct: 1   MKIPNLHFLFLIFTAVFFIAQSSLIDDVSSPEKRLAFFIFGDSLFDPGNNNFINTTEDFR 60

Query: 61  ANFTPYGETFFKYPTGRFSDGRLIPDFIAEYANLPLIPAYLDPRNKLYIHGVNFASGGGG 120
           ANFTPYGE+FFK PTGRFSDGRL+PDF+AEYANLPLIPAYLDP NK YIHGVNFASGGGG
Sbjct: 61  ANFTPYGESFFKTPTGRFSDGRLVPDFVAEYANLPLIPAYLDPHNKRYIHGVNFASGGGG 120

Query: 121 ALVETHRGFAIDIETQLRYFMKVERSVRKKLGDSRAYSLFSNSVYMFSIGGNDYIVPFEG 180
           ALVETHRGFAIDIETQLRYF KVERS+RKKLGD RAY+LFSNSVY+FSIGGNDYIVPFEG
Sbjct: 121 ALVETHRGFAIDIETQLRYFKKVERSIRKKLGDWRAYNLFSNSVYLFSIGGNDYIVPFEG 180

Query: 181 STVLEKYTETEYVNMVLGNATAVLEEIYKKGGRKFAFVAVPPLGCLPHSRLMKE-GSHGS 240
           S + +KYTE EYVNMV+GNATAVLEEIYKKGGRKFAFVAVPPLGCLPH RL+K+ G HGS
Sbjct: 181 SPIFDKYTEREYVNMVIGNATAVLEEIYKKGGRKFAFVAVPPLGCLPHIRLVKKAGGHGS 240

Query: 241 CYDEASALARLHNKLLPAALHKLANKLQGFKYTFADTYTLLQNRIDNPSKYGNPHFLINP 300
           C+DE SAL RLHNKLLP AL KLA+KLQGFKYT  DTYT+LQNRIDNPSKYG        
Sbjct: 241 CWDEPSALVRLHNKLLPGALQKLADKLQGFKYTVGDTYTMLQNRIDNPSKYG-------- 300

Query: 301 SMGCFKEGKKACCGSGELRGIYTCGGMRGQKEFELCENPNEYLFFDSYHPNQRAYEQFAK 360
               FKE K ACCGSG+ RGIY+CGGMRG KEFELCENPNEYLFFDSYHPN+RAYEQFAK
Sbjct: 301 ----FKEEKTACCGSGKFRGIYSCGGMRGVKEFELCENPNEYLFFDSYHPNERAYEQFAK 360

Query: 361 LMWSGDAQVIKPYNLKQLFQYGSSQP 386
           LMWSGD+QVI PY+LKQ FQY SSQP
Sbjct: 361 LMWSGDSQVINPYSLKQFFQYASSQP 374

BLAST of CaUC09G158960 vs. ExPASy TrEMBL
Match: A0A6J1HHR5 (GDSL esterase/lipase 5-like OS=Cucurbita moschata OX=3662 GN=LOC111464186 PE=3 SV=1)

HSP 1 Score: 637.9 bits (1644), Expect = 2.8e-179
Identity = 308/384 (80.21%), Postives = 333/384 (86.72%), Query Frame = 0

Query: 1   MKISNFHFLFLIFSALFFIAQSSQINDVPSSQKRLAFFIFGDSFFDPGNNNFINTTQDFR 60
           MKISN HF F IF  + F +  SQI+DVPSS   +AFF+FGDSF DPGNNNFINTT+DFR
Sbjct: 5   MKISNVHFFFKIFFTILFTSHGSQIDDVPSSNNGVAFFVFGDSFLDPGNNNFINTTEDFR 64

Query: 61  ANFTPYGETFFKYPTGRFSDGRLIPDFIAEYANLPLIPAYLDPRNKLYIHGVNFASGGGG 120
           ANFTPYGETFF  PTGRFSDGRLIPDFIAEYANLPLIPAYLDP N LYIHGVNFASGGGG
Sbjct: 65  ANFTPYGETFFNSPTGRFSDGRLIPDFIAEYANLPLIPAYLDPHNNLYIHGVNFASGGGG 124

Query: 121 ALVETHRGFAIDIETQLRYFMKVERSVRKKLGDSRAYSLFSNSVYMFSIGGNDYIVPFEG 180
           ALVETH+GFAI+IETQLRYF KVERS+RKKLGD+RA SL S+SVYMFSIGGNDYIV FEG
Sbjct: 125 ALVETHQGFAINIETQLRYFKKVERSLRKKLGDARASSLLSDSVYMFSIGGNDYIVAFEG 184

Query: 181 STVLEKYTETEYVNMVLGNATAVLEEIYKKGGRKFAFVAVPPLGCLPHSRLMKEGSHGSC 240
           S VLEKYTE EYVNMV+GN T+VL+EIYKKGGRKFAF+AVPPLGC+PH+RLMK G HGSC
Sbjct: 185 SPVLEKYTEIEYVNMVIGNVTSVLQEIYKKGGRKFAFLAVPPLGCMPHTRLMKVGGHGSC 244

Query: 241 YDEASALARLHNKLLPAALHKLANKLQGFKYTFADTYTLLQNRIDNPSKYGNPHFLINPS 300
           +DEASALARLHNKLLPAAL KLA+ LQGFKYT ADTY +LQNRIDNPSKYG         
Sbjct: 245 WDEASALARLHNKLLPAALQKLAHDLQGFKYTLADTYNMLQNRIDNPSKYG--------- 304

Query: 301 MGCFKEGKKACCGSGELRGIYTCGGMRGQKEFELCENPNEYLFFDSYHPNQRAYEQFAKL 360
               K+G+ ACCGSGELRGIY+CGGMRGQ EFELCENPNEYLFFDSYHPN+RAYEQFAKL
Sbjct: 305 ---LKDGRTACCGSGELRGIYSCGGMRGQTEFELCENPNEYLFFDSYHPNERAYEQFAKL 364

Query: 361 MWSGDAQVIKPYNLKQLFQYGSSQ 385
           +W GDAQ I P NLKQ FQ GSSQ
Sbjct: 365 IWYGDAQFINPSNLKQFFQCGSSQ 376

BLAST of CaUC09G158960 vs. ExPASy TrEMBL
Match: A0A6J1KD54 (GDSL esterase/lipase 5-like OS=Cucurbita maxima OX=3661 GN=LOC111494533 PE=3 SV=1)

HSP 1 Score: 627.5 bits (1617), Expect = 3.8e-176
Identity = 302/384 (78.65%), Postives = 330/384 (85.94%), Query Frame = 0

Query: 1   MKISNFHFLFLIFSALFFIAQSSQINDVPSSQKRLAFFIFGDSFFDPGNNNFINTTQDFR 60
           MKISN HF F IF  + F +  SQI DVPSS   +AFF+FGDS  DPGNNNFINTT+DFR
Sbjct: 1   MKISNVHFFFKIFFTILFTSHGSQIGDVPSSHNGVAFFVFGDSLLDPGNNNFINTTEDFR 60

Query: 61  ANFTPYGETFFKYPTGRFSDGRLIPDFIAEYANLPLIPAYLDPRNKLYIHGVNFASGGGG 120
           ANFTPYGETFF  P GRFSDGRLIPDFIAEYANLPLIPAYLDP + LYIHGVNFASGGGG
Sbjct: 61  ANFTPYGETFFNSPIGRFSDGRLIPDFIAEYANLPLIPAYLDPHSNLYIHGVNFASGGGG 120

Query: 121 ALVETHRGFAIDIETQLRYFMKVERSVRKKLGDSRAYSLFSNSVYMFSIGGNDYIVPFEG 180
           ALVETH+GFAI+IETQLRYF KVERS+RKKLG +RA SL S+SVYMFSIGGNDYIV FEG
Sbjct: 121 ALVETHQGFAINIETQLRYFKKVERSLRKKLGGARASSLLSDSVYMFSIGGNDYIVAFEG 180

Query: 181 STVLEKYTETEYVNMVLGNATAVLEEIYKKGGRKFAFVAVPPLGCLPHSRLMKEGSHGSC 240
           S VLEK+TE EYVNMV+GN T+VL+EIYKKGGRKFAF+AVPPLGC+PH+RLMK G HGSC
Sbjct: 181 SPVLEKFTEIEYVNMVIGNVTSVLQEIYKKGGRKFAFLAVPPLGCMPHTRLMKVGGHGSC 240

Query: 241 YDEASALARLHNKLLPAALHKLANKLQGFKYTFADTYTLLQNRIDNPSKYGNPHFLINPS 300
           +DEASALARLHNKLLPAAL KL + LQGFKYT ADTY +LQNRIDNPSKYG         
Sbjct: 241 WDEASALARLHNKLLPAALQKLTHDLQGFKYTLADTYNMLQNRIDNPSKYG--------- 300

Query: 301 MGCFKEGKKACCGSGELRGIYTCGGMRGQKEFELCENPNEYLFFDSYHPNQRAYEQFAKL 360
              FK+G+ ACCGSGELRGIY+CGGMRGQ+EFELCENPNEYLFFDSYHPN+RAYEQFAKL
Sbjct: 301 ---FKDGRTACCGSGELRGIYSCGGMRGQREFELCENPNEYLFFDSYHPNERAYEQFAKL 360

Query: 361 MWSGDAQVIKPYNLKQLFQYGSSQ 385
           +W+GD Q I P NLKQ FQ GSSQ
Sbjct: 361 IWNGDDQFINPSNLKQFFQCGSSQ 372

BLAST of CaUC09G158960 vs. TAIR 10
Match: AT1G53920.1 (GDSL-motif lipase 5 )

HSP 1 Score: 347.8 bits (891), Expect = 1.1e-95
Identity = 180/382 (47.12%), Postives = 246/382 (64.40%), Query Frame = 0

Query: 3   ISNFHFLFLIFSALFFIAQSSQINDVPSSQKRLAFFIFGDSFFDPGNNNFINTTQDFRAN 62
           IS+F F  +  + LF   +SS            A F+FGDSF D GNNN+INTT   +AN
Sbjct: 15  ISSFIFFIVSSTILFLAGKSSAKISHNGDNNVTALFLFGDSFLDAGNNNYINTTTLDQAN 74

Query: 63  FTPYGETFFKYPTGRFSDGRLIPDFIAEYANLPLIPAYLDPRN-KLYIHGVNFASGGGGA 122
           F PYG+TFF  PTGRFSDGRLI DFIAEYANLPLIP +L+P N +  ++GVNFAS G GA
Sbjct: 75  FPPYGQTFFGLPTGRFSDGRLISDFIAEYANLPLIPPFLEPGNSQKKLYGVNFASAGAGA 134

Query: 123 LVETHRGFAIDIETQLRYFMKVERSVRKKLGDSRAYSLFSNSVYMFSIGGNDYIVPFEGS 182
           LVET +G  I++ TQL ++ KVER  R   G   +    S +VY+ SIG NDY   F  +
Sbjct: 135 LVETFQGSVINLRTQLDHYKKVERLWRTNFGKEESKKRISRAVYLISIGSNDYSSIFLTN 194

Query: 183 TVLEKYTETEYVNMVLGNATAVLEEIYKKGGRKFAFVAVPPLGCLPHSRLMKEGSHGSCY 242
             L   + +++V++V+GN T  + EIYK GGRKF F+ VP LGC P  R+++  +  SC 
Sbjct: 195 QSL-PISMSQHVDIVIGNLTTFIHEIYKIGGRKFGFLNVPDLGCFPALRILQPKNDDSCL 254

Query: 243 DEASALARLHNKLLPAALHKLANKLQGFKYTFADTYTLLQNRIDNPSKYGNPHFLINPSM 302
            +AS LA +HN+ L   L ++  +++GFK++  D    L+ R+ +PSK+G          
Sbjct: 255 RDASRLASMHNRALTNLLFQMQRQVKGFKFSLFDMNKSLRLRMQHPSKFG---------- 314

Query: 303 GCFKEGKKACCGSGELRGIYTCGGMRGQKEFELCENPNEYLFFDSYHPNQRAYEQFAKLM 362
             FKEG++ACCG+G+ RG+++CGG R  KE++LCENP +Y+F+DS H  Q  Y QFA L+
Sbjct: 315 --FKEGEEACCGTGKWRGVFSCGGKRIVKEYQLCENPKDYIFWDSLHLTQNTYNQFANLI 374

Query: 363 WSG----DAQVIKPYNLKQLFQ 380
           W+G    D+ V+ PYN+  LFQ
Sbjct: 375 WNGGHMSDSLVVGPYNINNLFQ 383

BLAST of CaUC09G158960 vs. TAIR 10
Match: AT5G40990.1 (GDSL lipase 1 )

HSP 1 Score: 342.8 bits (878), Expect = 3.6e-94
Identity = 177/374 (47.33%), Postives = 233/374 (62.30%), Query Frame = 0

Query: 10  FLIFSALFFIAQSSQINDVPSSQKRLAFFIFGDSFFDPGNNNFINTTQDFRANFTPYGET 69
           FL ++ +  I   + I++      + A F+FGDS FD GNNN+I+T    R+N+ PYG+T
Sbjct: 11  FLAYTIIISIGSINCIDNNNLVTNQSALFVFGDSVFDAGNNNYIDTLSSVRSNYWPYGQT 70

Query: 70  FFKYPTGRFSDGRLIPDFIAEYANLPLIPAYLDP--RNKLYIHGVNFASGGGGALVETHR 129
            FK PTGR SDGRLIPDFIAEYA LPLIP  L P   N  + +GVNFASGG GALV T  
Sbjct: 71  TFKSPTGRVSDGRLIPDFIAEYAWLPLIPPNLQPFNGNSQFAYGVNFASGGAGALVGTFS 130

Query: 130 GFAIDIETQLRYFMKVERSVRKKLGDSRAYSLFSNSVYMFSIGGNDYIVPF-EGSTVLEK 189
           G  I++ TQL  F KVE  +R KLGD+    + S +VY+F IG NDY  PF   S++ + 
Sbjct: 131 GLVINLRTQLNNFKKVEEMLRSKLGDAEGKRVISRAVYLFHIGLNDYQYPFTTNSSLFQS 190

Query: 190 YTETEYVNMVLGNATAVLEEIYKKGGRKFAFVAVPPLGCLPHSRLMKEGSHGSCYDEASA 249
            +  +YV+ V+GN T V +E+Y  GGRKF  +   P  C P S ++ +    SC+   + 
Sbjct: 191 ISNEKYVDYVVGNMTDVFKEVYNLGGRKFGILNTGPYDCAPASLVIDQTKIRSCFQPVTE 250

Query: 250 LARLHNKLLPAALHKLANKLQGFKYTFADTYTLLQNRIDNPSKYGNPHFLINPSMGCFKE 309
           L  +HN+ L   L +L ++L GFKY   D +T L  R+++PSKYG            FKE
Sbjct: 251 LINMHNEKLLNGLRRLNHELSGFKYALHDYHTSLSERMNDPSKYG------------FKE 310

Query: 310 GKKACCGSGELRGIYTCGGMRG-QKEFELCENPNEYLFFDSYHPNQRAYEQFAKLMWSGD 369
           GKKACCGSG LRGI TCGG  G  + +ELCEN  +YLFFD +H  ++A  Q A+L+WSG 
Sbjct: 311 GKKACCGSGPLRGINTCGGRMGLSQSYELCENVTDYLFFDPFHLTEKANRQIAELIWSGP 370

Query: 370 AQVIKPYNLKQLFQ 380
             +  PYNLK LF+
Sbjct: 371 TNITGPYNLKALFE 372

BLAST of CaUC09G158960 vs. TAIR 10
Match: AT1G53940.1 (GDSL-motif lipase 2 )

HSP 1 Score: 329.3 bits (843), Expect = 4.1e-90
Identity = 172/371 (46.36%), Postives = 233/371 (62.80%), Query Frame = 0

Query: 3   ISNFHFLFLIFSALFFIAQSSQINDVPSSQKRL-----AFFIFGDSFFDPGNNNFINTTQ 62
           + N     +IF A   I     IN   ++   L     A F+FGDS FD GNNN+I+T  
Sbjct: 1   MENSRSTLIIFFAYTTIILIGSINCRDNNNNNLVTNQSALFVFGDSVFDAGNNNYIDTLP 60

Query: 63  DFRANFTPYGETFFKYPTGRFSDGRLIPDFIAEYANLPLIPAYLDPRN--KLYIHGVNFA 122
            FR+N+ PYG+T FK+PTGR SDGR IPDFIAEYA LPLIPAYL P N    + +GV+FA
Sbjct: 61  SFRSNYWPYGQTTFKFPTGRVSDGRTIPDFIAEYAWLPLIPAYLQPSNGKNQFPYGVSFA 120

Query: 123 SGGGGALVETHRGFAIDIETQLRYFMKVERSVRKKLGDSRAYSLFSNSVYMFSIGGNDYI 182
           S G GALV T  G  I++++QL  F KVE+ +R  LG+++   + S +VY+F IG NDY 
Sbjct: 121 SAGAGALVGTFPGMVINLKSQLNNFKKVEKLLRSTLGEAQGKMVISRAVYLFHIGVNDYQ 180

Query: 183 VPFE-GSTVLEKYTETEYVNMVLGNATAVLEEIYKKGGRKFAFVAVPPLGCLPHSRLMKE 242
            PF   S++ +   +  YV+ V+GN TAV++E+YK GGRKF F+ +    C P S ++ +
Sbjct: 181 YPFSTNSSIFQSSPQEIYVDFVVGNTTAVIKEVYKIGGRKFGFLNMGAYDCAPASLIIDQ 240

Query: 243 GSHGSCYDEASALARLHNKLLPAALHKLANKLQGFKYTFADTYTLLQNRIDNPSKYGNPH 302
              G+C+   + L  LHN+ L + L +L  +L GFKY   D +T L  R++NPSKYG   
Sbjct: 241 TKIGTCFKPVTELINLHNEKLESGLRRLERELSGFKYALHDYHTSLSVRMNNPSKYG--- 300

Query: 303 FLINPSMGCFKEGKKACCGSGELRGIYTCGGMRG-QKEFELCENPNEYLFFDSYHPNQRA 362
                    FKEGK ACCG+G LRGI TCGG  G  + +ELCE   +YLFFD +H  ++A
Sbjct: 301 ---------FKEGKMACCGTGPLRGINTCGGRMGVSQSYELCEKVTDYLFFDHFHLTEKA 359

Query: 363 YEQFAKLMWSG 365
           ++Q A+L+WSG
Sbjct: 361 HQQIAELIWSG 359

BLAST of CaUC09G158960 vs. TAIR 10
Match: AT1G53990.1 (GDSL-motif lipase 3 )

HSP 1 Score: 327.0 bits (837), Expect = 2.0e-89
Identity = 168/375 (44.80%), Postives = 229/375 (61.07%), Query Frame = 0

Query: 9   LFLIFSALFFIAQSSQINDVPSSQKRLAFFIFGDSFFDPGNNNFINTTQDFRANFTPYGE 68
           +F +++ +  I   + I++      + A F+FGDS FD GNNN+INT   FR+N  PYG+
Sbjct: 8   IFFVYTIILSIGSINCIDNNNLVTNQAALFVFGDSLFDAGNNNYINTVSSFRSNIWPYGQ 67

Query: 69  TFFKYPTGRFSDGRLIPDFIAEYANLPLIPAYLDPR--NKLYIHGVNFASGGGGALVETH 128
           T FK+PTGR SDG        E A LP IP  L P   N  + +GV+FAS G GAL E+ 
Sbjct: 68  TNFKFPTGRLSDG-------PEKAWLPSIPPNLQPNNGNNQFTYGVSFASAGAGALAESF 127

Query: 129 RGFAIDIETQLRYFMKVERSVRKKLGDSRAYSLFSNSVYMFSIGGNDYIVPFE-GSTVLE 188
            G  I++ TQL  F  VE+S+R +LGD+    +FS +VY+F IG NDY  PF   S+  +
Sbjct: 128 LGMVINLGTQLNNFKDVEKSLRSELGDAETKRVFSRAVYLFHIGANDYFYPFSANSSTFK 187

Query: 189 KYTETEYVNMVLGNATAVLEEIYKKGGRKFAFVAVPPLGCLPHSRLMKEGSHGSCYDEAS 248
             ++ ++V+ V+GN T V+EE+YK GGRKF F+ V P  C P+S +      GSC+   +
Sbjct: 188 SNSKEKFVDFVIGNITFVIEEVYKMGGRKFGFLNVGPYECSPNSLIRDRTKIGSCFKPVA 247

Query: 249 ALARLHNKLLPAALHKLANKLQGFKYTFADTYTLLQNRIDNPSKYGNPHFLINPSMGCFK 308
            L  +HNK  P  L +L  +L GF+Y   D +T L  RI++PSKYG            FK
Sbjct: 248 ELIDMHNKKFPDVLRRLQRQLSGFRYALHDYHTSLSERINSPSKYG------------FK 307

Query: 309 EGKKACCGSGELRGIYTCGGMRGQKE-FELCENPNEYLFFDSYHPNQRAYEQFAKLMWSG 368
           EGKKACCGSG LRGI TCG   G  + + LCEN  +YLF+DS H  ++A+ Q A+L+W+G
Sbjct: 308 EGKKACCGSGPLRGINTCGNRIGPSQGYGLCENVTDYLFYDSSHLTEKAHRQIAELIWNG 363

Query: 369 DAQVIKPYNLKQLFQ 380
              V +PYNLK LF+
Sbjct: 368 PPNVTRPYNLKALFE 363

BLAST of CaUC09G158960 vs. TAIR 10
Match: AT3G14225.1 (GDSL-motif lipase 4 )

HSP 1 Score: 320.9 bits (821), Expect = 1.4e-87
Identity = 167/379 (44.06%), Postives = 232/379 (61.21%), Query Frame = 0

Query: 9   LFLIFSALFFIAQSSQINDVPSSQKRLAFFIFGDSFFDPGNNNFINTTQDFRANFTPYGE 68
           LF+   +L  ++ S +  D+ ++Q   A F FGDS F+ GNNN+ ++   FR+NF PYG+
Sbjct: 13  LFICTISLSIVSISCK-EDLKTNQ--AALFAFGDSLFEAGNNNYFDSISSFRSNFWPYGK 72

Query: 69  TFFKYPTGRFSDGRLIPDFIAEYANLPLIPAYLDP--RNKLYIHGVNFASGGGGALVETH 128
           T FK+PTGR SDGR++ DFIAEYA LPLIP  L P   N    +G+NFA+   G    T 
Sbjct: 73  TTFKFPTGRVSDGRIMIDFIAEYAWLPLIPPNLQPGYSNSQLTYGLNFATTAAGVFAGTF 132

Query: 129 RG----FAIDIETQLRYFMKVERSVRKKLGDSRAYSLFSNSVYMFSIGGNDYIVPFEGST 188
            G     + D+ TQL  F  VE+++R  LGD+ A  + S +VY+F IG NDY  PF  +T
Sbjct: 133 PGSVTNLSKDLGTQLNNFKNVEKTLRSNLGDAEARRVISKAVYLFHIGANDYQYPFFANT 192

Query: 189 -VLEKYTETEYVNMVLGNATAVLEEIYKKGGRKFAFVAVPPLGCLPHSRLMKEGSHGSCY 248
                 T+  +++ V+GN T V+EE+YK G RKF F+++ P GC P + ++     GSC+
Sbjct: 193 STFSNTTKERFIDFVIGNTTTVIEELYKLGARKFGFLSLGPFGCTPSALIINSTKIGSCF 252

Query: 249 DEASALARLHNKLLPAALHKLANKLQGFKYTFADTYTLLQNRIDNPSKYGNPHFLINPSM 308
           +  + L  LHN+  P  L +L  +L GFKY   D +T L  RI+NPS+YG          
Sbjct: 253 EPVTELINLHNQEFPKVLRRLERRLSGFKYALHDFHTSLSQRINNPSRYG---------- 312

Query: 309 GCFKEGKKACCGSGELRGIYTCGGMRGQKE-FELCENPNEYLFFDSYHPNQRAYEQFAKL 368
             FKEG+ ACCGSG LRGI TCG   G  + ++LCEN ++Y+FFD  H  + A++Q A+L
Sbjct: 313 --FKEGEMACCGSGPLRGINTCGFRNGPSQGYKLCENADDYVFFDPSHLTETAHQQIAEL 372

Query: 369 MWSGDAQVIKPYNLKQLFQ 380
           +WSG   V  PYNLK LF+
Sbjct: 373 IWSGPPNVTAPYNLKTLFR 376

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038897092.14.0e-19688.02GDSL lipase-like [Benincasa hispida][more]
XP_008462253.19.4e-19084.42PREDICTED: GDSL esterase/lipase 5-like isoform X1 [Cucumis melo] >KAA0059365.1 G... [more]
XP_004141726.11.7e-18682.90GDSL lipase isoform X1 [Cucumis sativus] >KGN45451.1 hypothetical protein Csa_01... [more]
XP_022964041.15.7e-17980.21GDSL esterase/lipase 5-like [Cucurbita moschata][more]
XP_023515160.12.2e-17879.43GDSL esterase/lipase 5-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
H6U1I81.2e-9951.71GDSL lipase OS=Tanacetum cinerariifolium OX=118510 GN=GLIP PE=1 SV=1[more]
Q9SSA71.6e-9447.12GDSL esterase/lipase 5 OS=Arabidopsis thaliana OX=3702 GN=GLIP5 PE=2 SV=2[more]
Q9SYF04.5e-9446.63GDSL esterase/lipase 2 OS=Arabidopsis thaliana OX=3702 GN=GLIP2 PE=2 SV=1[more]
Q9FLN05.0e-9347.33GDSL esterase/lipase 1 OS=Arabidopsis thaliana OX=3702 GN=GLIP1 PE=1 SV=1[more]
Q9SYF52.8e-8844.80GDSL esterase/lipase 3 OS=Arabidopsis thaliana OX=3702 GN=GLIP3 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A5A7V0K24.6e-19084.42GDSL esterase/lipase 5-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A1S3CI174.6e-19084.42GDSL esterase/lipase 5-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103500655 P... [more]
A0A0A0KCD48.1e-18782.90Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G448130 PE=3 SV=1[more]
A0A6J1HHR52.8e-17980.21GDSL esterase/lipase 5-like OS=Cucurbita moschata OX=3662 GN=LOC111464186 PE=3 S... [more]
A0A6J1KD543.8e-17678.65GDSL esterase/lipase 5-like OS=Cucurbita maxima OX=3661 GN=LOC111494533 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
AT1G53920.11.1e-9547.12GDSL-motif lipase 5 [more]
AT5G40990.13.6e-9447.33GDSL lipase 1 [more]
AT1G53940.14.1e-9046.36GDSL-motif lipase 2 [more]
AT1G53990.12.0e-8944.80GDSL-motif lipase 3 [more]
AT3G14225.11.4e-8744.06GDSL-motif lipase 4 [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (USVL246-FR2) v1
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036514SGNH hydrolase superfamilyGENE3D3.40.50.1110SGNH hydrolasecoord: 24..366
e-value: 3.1E-70
score: 239.1
IPR001087GDSL lipase/esterasePFAMPF00657Lipase_GDSLcoord: 38..360
e-value: 2.2E-30
score: 106.8
IPR044552GDSL esterase/lipase GLIP1-5/GLL25PANTHERPTHR45966GDSL-LIKE LIPASE/ACYLHYDROLASEcoord: 7..379
NoneNo IPR availablePANTHERPTHR45966:SF4GDSL ESTERASE/LIPASE 5coord: 7..379
NoneNo IPR availableSUPERFAMILY52266SGNH hydrolasecoord: 37..363
IPR035669GDSL lipase/esterase-like, plantCDDcd01837SGNH_plant_lipase_likecoord: 36..365
e-value: 1.45912E-124
score: 360.007

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CaUC09G158960.1CaUC09G158960.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016298 lipase activity
molecular_function GO:0016788 hydrolase activity, acting on ester bonds