CaUC08G157650 (gene) Watermelon (USVL246-FR2) v1

Overview


Name	CaUC08G157650
Type	gene
Organism	Citrullus amarus (Watermelon (USVL246-FR2) v1)
Description	protein NUCLEAR FUSION DEFECTIVE 6, chloroplastic/mitochondrial
Location	Ciama_Chr08: 28783397 .. 28784321 (+)
RNA-Seq Expression	CaUC08G157650
Synteny	CaUC08G157650

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GTCTCAGGCTCTACCGGATAGCTCTTCTCTTCTTTACTTCCAAATTTTGGCTTGAAACTTGAAAGTAAGGGAACATGGCGGCTTGCTATTCCTGGAAAACCCTACGAATTTCCTCCTCTTCCGCTAGAACTCTCCTTCACCGTTCCTCCTCCTTTACCTCTGCATCTCATTTTGTGTCCAAACCTACTATGTCTGCCGGACTTCCTTCCGCAAAGCCGCCCGCATCTTCCCGCTTCTCTGTTCAGAAGCTTACCAACTTCAGGTTATTTATTCATTTTTCCTTTTTTGGTATTCGTTCTTTTTCGATTTGGAGGTATGTTGGAACTATATGAATCAGTAATGTAATGTCGTCTTCTTCGGCTCCAAGATTTGGTATTTCTCAATTTCAATGGCCATAACTTTTTATTTTTGCCTTTTTTTAAGTTAGTTTCTTGGATTTAGTCCATGTTTTTTTCTGCTTCTATTTCCATAAAGAGGCGACCATTTTTCTTTTCTAAATCTAAGTATTGACCCCAGTTCTTTAAAGTACACTTACTGGGTTAGCTTATCTATTTTTACCCATTCACGTTCAATCTATGGGATTCTATGCATATCTACTTACCGAAGATAATCATCATGACGACTCAGTGATTTTATGCTAAATGTCGCTGCTTCTTTAGGCTTCCAGTGGAGTTATCAAGCTTGCAGTCTCTAATGCCATTGCACAGTGTTACTGCTTCTGCCTTGTTTACTTCGTTGCTTTCTTTGCATAACAACAGTTGGGGTTGTCTATCTGAAGGTAACCTTTCTAGTTTTAGGCTTTTAGCTCATTCCATATTGTCAAAATTAGCCCACCTAAAATTTCAAAAATTTACTCTTCGATAAGATATTAAGATCTTTGAGGTTTATTGTAGTTTTACGATTCTCCAAGTTATTTGCAATCTGA

mRNA sequence

GTCTCAGGCTCTACCGGATAGCTCTTCTCTTCTTTACTTCCAAATTTTGGCTTGAAACTTGAAAGTAAGGGAACATGGCGGCTTGCTATTCCTGGAAAACCCTACGAATTTCCTCCTCTTCCGCTAGAACTCTCCTTCACCGTTCCTCCTCCTTTACCTCTGCATCTCATTTTGTGTCCAAACCTACTATGTCTGCCGGACTTCCTTCCGCAAAGCCGCCCGCATCTTCCCGCTTCTCTGTTCAGAAGCTTACCAACTTCAGGCTTCCAGTGGAGTTATCAAGCTTGCAGTCTCTAATGCCATTGCACAGTGTTACTGCTTCTGCCTTGTTTACTTCGTTGCTTTCTTTGCATAACAACAGTTGGGGTTGTCTATCTGAAGTTTTACGATTCTCCAAGTTATTTGCAATCTGA

Coding sequence (CDS)

ATGGCGGCTTGCTATTCCTGGAAAACCCTACGAATTTCCTCCTCTTCCGCTAGAACTCTCCTTCACCGTTCCTCCTCCTTTACCTCTGCATCTCATTTTGTGTCCAAACCTACTATGTCTGCCGGACTTCCTTCCGCAAAGCCGCCCGCATCTTCCCGCTTCTCTGTTCAGAAGCTTACCAACTTCAGGCTTCCAGTGGAGTTATCAAGCTTGCAGTCTCTAATGCCATTGCACAGTGTTACTGCTTCTGCCTTGTTTACTTCGTTGCTTTCTTTGCATAACAACAGTTGGGGTTGTCTATCTGAAGTTTTACGATTCTCCAAGTTATTTGCAATCTGA

Protein sequence

MAACYSWKTLRISSSSARTLLHRSSSFTSASHFVSKPTMSAGLPSAKPPASSRFSVQKLTNFRLPVELSSLQSLMPLHSVTASALFTSLLSLHNNSWGCLSEVLRFSKLFAI

Homology

[Close]

BLAST of CaUC08G157650 vs. NCBI nr
Match: XP_038885251.1 (protein NUCLEAR FUSION DEFECTIVE 6, mitochondrial isoform X2 [Benincasa hispida])

HSP 1 Score: 176.0 bits (445), Expect = 1.8e-40
Identity = 96/102 (94.12%), Postives = 97/102 (95.10%), Query Frame = 0


Query: 1   MAACYSWKTLRISSSSARTLLHRSSSFTSASHFVSKPTMSAGLPSAKPPASSRFSVQKLT 60
           MAAC SWKTLRISSSSARTLLHRSSS  SASHFVSKPTMSAGLPSAKPPASSRF VQKLT
Sbjct: 1   MAACCSWKTLRISSSSARTLLHRSSSSPSASHFVSKPTMSAGLPSAKPPASSRFYVQKLT 60

Query: 61  NFRLPVELSSLQSLMPLHSVTASALFTSLLSLHNNSWGCLSE 103
           NFRLPVELSS+QSLMPLHSVTASALFTSLLSLHNNSWG LSE
Sbjct: 61  NFRLPVELSSVQSLMPLHSVTASALFTSLLSLHNNSWGYLSE 102

[Close]

BLAST of CaUC08G157650 vs. NCBI nr
Match: XP_022996536.1 (uncharacterized protein LOC111491756 isoform X2 [Cucurbita maxima] >XP_023546953.1 uncharacterized protein LOC111805899 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 166.4 bits (420), Expect = 1.4e-37
Identity = 91/104 (87.50%), Postives = 94/104 (90.38%), Query Frame = 0


Query: 1   MAACYSWKTLRISSSSARTLLHRSSSFTSASHFVSKPTMSAGLPSAKPPASSRFSVQKLT 60
           MAAC +WKTLRISSSSARTLL RSSS  SASH VSKPTMSA LPSAKP ASSRFSV KLT
Sbjct: 1   MAACSTWKTLRISSSSARTLLQRSSSSPSASHLVSKPTMSAPLPSAKPSASSRFSVPKLT 60

Query: 61  NFRLPVELSSLQSLMPLHSVTASALFTSLLSLHNNSWGCLSEVL 105
           NFRLPVELSS+QSLMPLHS TASALFTSLLSLHNNSWGCLSE +
Sbjct: 61  NFRLPVELSSVQSLMPLHSATASALFTSLLSLHNNSWGCLSEAM 104

[Close]

BLAST of CaUC08G157650 vs. NCBI nr
Match: XP_022961783.1 (protein NUCLEAR FUSION DEFECTIVE 6, chloroplastic/mitochondrial [Cucurbita moschata] >KAG6598206.1 hypothetical protein SDJN03_07984, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 161.4 bits (407), Expect = 4.6e-36
Identity = 91/103 (88.35%), Postives = 93/103 (90.29%), Query Frame = 0


Query: 1   MAACYSWKTLRISSSSARTLLHRSSSF-TSASHFVSKPTMSAGLPSAKPPASSRFSVQKL 60
           MAAC +WKTLRISSSSARTLL RSSS   SASH VSKPTMSA LPSAKP ASSRFSV KL
Sbjct: 1   MAACSTWKTLRISSSSARTLLQRSSSSPPSASHLVSKPTMSAPLPSAKPSASSRFSVPKL 60

Query: 61  TNFRLPVELSSLQSLMPLHSVTASALFTSLLSLHNNSWGCLSE 103
           TNFRLPVELSS+QSLMPLHS TASALFTSLLSLHNNSWGCLSE
Sbjct: 61  TNFRLPVELSSVQSLMPLHSATASALFTSLLSLHNNSWGCLSE 103

[Close]

BLAST of CaUC08G157650 vs. NCBI nr
Match: KAG7029191.1 (hypothetical protein SDJN02_07528, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 161.4 bits (407), Expect = 4.6e-36
Identity = 91/103 (88.35%), Postives = 93/103 (90.29%), Query Frame = 0


Query: 1   MAACYSWKTLRISSSSARTLLHRSSSF-TSASHFVSKPTMSAGLPSAKPPASSRFSVQKL 60
           MAAC +WKTLRISSSSARTLL RSSS   SASH VSKPTMSA LPSAKP ASSRFSV KL
Sbjct: 44  MAACSTWKTLRISSSSARTLLQRSSSSPPSASHLVSKPTMSAPLPSAKPSASSRFSVPKL 103

Query: 61  TNFRLPVELSSLQSLMPLHSVTASALFTSLLSLHNNSWGCLSE 103
           TNFRLPVELSS+QSLMPLHS TASALFTSLLSLHNNSWGCLSE
Sbjct: 104 TNFRLPVELSSVQSLMPLHSATASALFTSLLSLHNNSWGCLSE 146

[Close]

BLAST of CaUC08G157650 vs. NCBI nr
Match: XP_004145570.1 (uncharacterized protein LOC101210124 [Cucumis sativus] >KGN55566.1 hypothetical protein Csa_012846 [Cucumis sativus])

HSP 1 Score: 161.4 bits (407), Expect = 4.6e-36
Identity = 86/104 (82.69%), Postives = 92/104 (88.46%), Query Frame = 0


Query: 1   MAACYSWKTLRISSSSARTLLHRSSSFTSASHFVSKPTMSAGLPSAKPPASSRFSVQKLT 60
           MAAC SWKT+ ISSSSAR LLHRSSS  SASHFVS PTMS GLPSAKPPASSRFSV +LT
Sbjct: 1   MAACCSWKTIPISSSSARGLLHRSSSSPSASHFVSNPTMSGGLPSAKPPASSRFSVHRLT 60

Query: 61  NFRLPVELSSLQSLMPLHSVTASALFTSLLSLHNNSWGCLSEVL 105
           NFRLPVEL+S+QSLMPL SVTAS LFTSLLS HNNSWGCLS+ +
Sbjct: 61  NFRLPVELASMQSLMPLRSVTASPLFTSLLSFHNNSWGCLSKAM 104

[Close]

BLAST of CaUC08G157650 vs. ExPASy TrEMBL
Match: A0A6J1K524 (uncharacterized protein LOC111491756 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111491756 PE=4 SV=1)

HSP 1 Score: 166.4 bits (420), Expect = 6.9e-38
Identity = 91/104 (87.50%), Postives = 94/104 (90.38%), Query Frame = 0


Query: 1   MAACYSWKTLRISSSSARTLLHRSSSFTSASHFVSKPTMSAGLPSAKPPASSRFSVQKLT 60
           MAAC +WKTLRISSSSARTLL RSSS  SASH VSKPTMSA LPSAKP ASSRFSV KLT
Sbjct: 1   MAACSTWKTLRISSSSARTLLQRSSSSPSASHLVSKPTMSAPLPSAKPSASSRFSVPKLT 60

Query: 61  NFRLPVELSSLQSLMPLHSVTASALFTSLLSLHNNSWGCLSEVL 105
           NFRLPVELSS+QSLMPLHS TASALFTSLLSLHNNSWGCLSE +
Sbjct: 61  NFRLPVELSSVQSLMPLHSATASALFTSLLSLHNNSWGCLSEAM 104

[Close]

BLAST of CaUC08G157650 vs. ExPASy TrEMBL
Match: A0A0A0L605 (Radical_SAM domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G667190 PE=4 SV=1)

HSP 1 Score: 161.4 bits (407), Expect = 2.2e-36
Identity = 86/104 (82.69%), Postives = 92/104 (88.46%), Query Frame = 0


Query: 1   MAACYSWKTLRISSSSARTLLHRSSSFTSASHFVSKPTMSAGLPSAKPPASSRFSVQKLT 60
           MAAC SWKT+ ISSSSAR LLHRSSS  SASHFVS PTMS GLPSAKPPASSRFSV +LT
Sbjct: 1   MAACCSWKTIPISSSSARGLLHRSSSSPSASHFVSNPTMSGGLPSAKPPASSRFSVHRLT 60

Query: 61  NFRLPVELSSLQSLMPLHSVTASALFTSLLSLHNNSWGCLSEVL 105
           NFRLPVEL+S+QSLMPL SVTAS LFTSLLS HNNSWGCLS+ +
Sbjct: 61  NFRLPVELASMQSLMPLRSVTASPLFTSLLSFHNNSWGCLSKAM 104

[Close]

BLAST of CaUC08G157650 vs. ExPASy TrEMBL
Match: A0A6J1HCT7 (protein NUCLEAR FUSION DEFECTIVE 6, chloroplastic/mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111462447 PE=4 SV=1)

HSP 1 Score: 161.4 bits (407), Expect = 2.2e-36
Identity = 91/103 (88.35%), Postives = 93/103 (90.29%), Query Frame = 0


Query: 1   MAACYSWKTLRISSSSARTLLHRSSSF-TSASHFVSKPTMSAGLPSAKPPASSRFSVQKL 60
           MAAC +WKTLRISSSSARTLL RSSS   SASH VSKPTMSA LPSAKP ASSRFSV KL
Sbjct: 1   MAACSTWKTLRISSSSARTLLQRSSSSPPSASHLVSKPTMSAPLPSAKPSASSRFSVPKL 60

Query: 61  TNFRLPVELSSLQSLMPLHSVTASALFTSLLSLHNNSWGCLSE 103
           TNFRLPVELSS+QSLMPLHS TASALFTSLLSLHNNSWGCLSE
Sbjct: 61  TNFRLPVELSSVQSLMPLHSATASALFTSLLSLHNNSWGCLSE 103

[Close]

BLAST of CaUC08G157650 vs. ExPASy TrEMBL
Match: A0A6J1E309 (protein NUCLEAR FUSION DEFECTIVE 6, chloroplastic/mitochondrial OS=Momordica charantia OX=3673 GN=LOC111025568 PE=4 SV=1)

HSP 1 Score: 160.2 bits (404), Expect = 4.9e-36
Identity = 86/102 (84.31%), Postives = 92/102 (90.20%), Query Frame = 0


Query: 1   MAACYSWKTLRISSSSARTLLHRSSSFTSASHFVSKPTMSAGLPSAKPPASSRFSVQKLT 60
           MAAC +WKTLR+SSSS RTL+ RSSS +SASHFVS+PT   GLPSAKP AS RFSVQKLT
Sbjct: 1   MAACCTWKTLRMSSSSTRTLICRSSSSSSASHFVSRPTKPTGLPSAKPAASPRFSVQKLT 60

Query: 61  NFRLPVELSSLQSLMPLHSVTASALFTSLLSLHNNSWGCLSE 103
           N RLPVELSS+QSLMPLHSVTASALFTSLLSLHNNSWGCLSE
Sbjct: 61  NLRLPVELSSVQSLMPLHSVTASALFTSLLSLHNNSWGCLSE 102

[Close]

BLAST of CaUC08G157650 vs. ExPASy TrEMBL
Match: A0A5D3D8K9 (Dual-specificity RNA methyltransferase RlmN isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G00010 PE=4 SV=1)

HSP 1 Score: 144.4 bits (363), Expect = 2.8e-31
Identity = 81/104 (77.88%), Postives = 88/104 (84.62%), Query Frame = 0


Query: 1   MAACYSWKTLRISSSSARTLLHRSSSFTSASHFVSKPTMSAGLPSAKPPASSRFSVQKLT 60
           MAAC SWKT+ ISSSSAR LLHR SS  SASHFVS PTMS GL SA+P ASSRFSVQ+LT
Sbjct: 1   MAACCSWKTIPISSSSARALLHRPSSSPSASHFVSIPTMSGGLHSARPAASSRFSVQRLT 60

Query: 61  NFRLPVELSSLQSLMPLHSVTASALFTSLLSLHNNSWGCLSEVL 105
           NF LPVEL+S+QSLMPL SVTASALFTSLLS HNNS  CLS+ +
Sbjct: 61  NFSLPVELASMQSLMPLRSVTASALFTSLLSFHNNSRRCLSKAM 104

[Close]

BLAST of CaUC08G157650 vs. TAIR 10
Match: AT1G70350.1 (unknown protein; Has 98 Blast hits to 98 proteins in 11 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 98; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 74.7 bits (182), Expect = 5.2e-14
Identity = 53/102 (51.96%), Postives = 69/102 (67.65%), Query Frame = 0


Query: 1   MAACYSWKTLRISSSSARTLLHRSSSFTSASHFVSKPTMSAGLPSAKPPASSRFSVQKLT 60
           MAA    + L+ SSSS + L  RSS+ TSAS       ++ G  S+   +S+R S+++LT
Sbjct: 1   MAANGVRRALQASSSSGKILFGRSSAATSASKIGKSAGIAFGNGSS---SSTRPSLRRLT 60

Query: 61  NFRLPVELSSLQSLMPLHSVTASALFTSLLSLHNNSWGCLSE 103
             R+PVELS+  SL+PLHSVTASAL TSLLSL N +W CLSE
Sbjct: 61  FSRVPVELSAGISLIPLHSVTASALLTSLLSLSNQNWSCLSE 99

[Close]

BLAST of CaUC08G157650 vs. TAIR 10
Match: AT1G55205.2 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G13674.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 46.2 bits (108), Expect = 2.0e-05
Identity = 35/98 (35.71%), Postives = 54/98 (55.10%), Query Frame = 0


Query: 15  SSARTLLHRSSSFTSASHFVSKPTMS-AGLPSAKPPASSRFSVQKLT-NFRLPVELSSLQ 74
           +S+  +   SS   S +  +SK  +  + LPS    +S   +  ++  +FRLPVELS   
Sbjct: 2   ASSSKICRLSSKIHSLTQKLSKTNVHISSLPSPIKSSSPSSATSRINQSFRLPVELSCCL 61

Query: 75  SLMPLHSVTASALFTSLLSLHNNSWGCLSEVLRFSKLF 111
           S++PLHS  ASA   S LS+ + SWG + +   FS L+
Sbjct: 62  SMLPLHSAIASARLISSLSVESKSWGLVPQGQSFSSLW 99

[Close]

BLAST of CaUC08G157650 vs. TAIR 10
Match: AT1G55205.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G13674.1); Has 137 Blast hits to 137 proteins in 11 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 137; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 43.1 bits (100), Expect = 1.7e-04
Identity = 32/90 (35.56%), Postives = 50/90 (55.56%), Query Frame = 0


Query: 15  SSARTLLHRSSSFTSASHFVSKPTMS-AGLPSAKPPASSRFSVQKLT-NFRLPVELSSLQ 74
           +S+  +   SS   S +  +SK  +  + LPS    +S   +  ++  +FRLPVELS   
Sbjct: 2   ASSSKICRLSSKIHSLTQKLSKTNVHISSLPSPIKSSSPSSATSRINQSFRLPVELSCCL 61

Query: 75  SLMPLHSVTASALFTSLLSLHNNSWGCLSE 103
           S++PLHS  ASA   S LS+ + SWG + +
Sbjct: 62  SMLPLHSAIASARLISSLSVESKSWGLVPQ 91

The following BLAST results are available for this feature:

BLAST of CaUC08G157650 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038885251.1	1.8e-40	94.12	protein NUCLEAR FUSION DEFECTIVE 6, mitochondrial isoform X2 [Benincasa hispida]	[more]
XP_022996536.1	1.4e-37	87.50	uncharacterized protein LOC111491756 isoform X2 [Cucurbita maxima] >XP_023546953...	[more]
XP_022961783.1	4.6e-36	88.35	protein NUCLEAR FUSION DEFECTIVE 6, chloroplastic/mitochondrial [Cucurbita mosch...	[more]
KAG7029191.1	4.6e-36	88.35	hypothetical protein SDJN02_07528, partial [Cucurbita argyrosperma subsp. argyro...	[more]
XP_004145570.1	4.6e-36	82.69	uncharacterized protein LOC101210124 [Cucumis sativus] >KGN55566.1 hypothetical ...	[more]

BLAST of CaUC08G157650 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of CaUC08G157650 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1K524	6.9e-38	87.50	uncharacterized protein LOC111491756 isoform X2 OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A0A0L605	2.2e-36	82.69	Radical_SAM domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G667190...	[more]
A0A6J1HCT7	2.2e-36	88.35	protein NUCLEAR FUSION DEFECTIVE 6, chloroplastic/mitochondrial OS=Cucurbita mos...	[more]
A0A6J1E309	4.9e-36	84.31	protein NUCLEAR FUSION DEFECTIVE 6, chloroplastic/mitochondrial OS=Momordica cha...	[more]
A0A5D3D8K9	2.8e-31	77.88	Dual-specificity RNA methyltransferase RlmN isoform X1 OS=Cucumis melo var. maku...	[more]

BLAST of CaUC08G157650 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G70350.1	5.2e-14	51.96	unknown protein; Has 98 Blast hits to 98 proteins in 11 species: Archae - 0; Bac...	[more]
AT1G55205.2	2.0e-05	35.71	unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic...	[more]
AT1G55205.1	1.7e-04	35.56	unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic...	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (USVL246-FR2) v1
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR043459	NFD6/NOXY2-like	PANTHER	PTHR33156	OS02G0230000 PROTEIN	coord: 1..103
None	No IPR available	PANTHER	PTHR33156:SF2	OS01G0738000 PROTEIN	coord: 1..103

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CaUC08G157650.1	CaUC08G157650.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0070475	rRNA base methylation
biological_process	GO:0030488	tRNA methylation
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0051539	4 iron, 4 sulfur cluster binding
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0008173	RNA methyltransferase activity