CaUC08G155270 (gene) Watermelon (USVL246-FR2) v1

Overview
NameCaUC08G155270
Typegene
OrganismCitrullus amarus (Watermelon (USVL246-FR2) v1)
DescriptionDPH4-like protein
LocationCiama_Chr08: 26828639 .. 26831657 (-)
RNA-Seq ExpressionCaUC08G155270
SyntenyCaUC08G155270
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCTCTCCATTTTCACGCCAGCTTCCCGTTGCGTCGCACCACCCCGCCGCCGCCATCTCCGTTGGGCTCAACGCCGCACCCTTTCTTCGCCGATCTTCTCCCCTCCGTTAGTCTCAGGTATTTATTATCTTACATATATATCTTCGAGTTTCATTCTTTACAATGGATGAGCTTCTGTAAGCTCAAACGAACGAAGATCGGTCCGTTTGTGAATCGATTTTTCCTAATCAAGGACTTTGGACATTCGTTTGGTTGTTCATTCTTACAATCGAGATTATTGGTTTGATAATCAGTTTAGCCAAAAGATCATAGAACCGAAGCTTGTGCTAAATGATGGTTTTTATGTAATGGAAGAAGCTTGAATTGAATATCCAGTAGCTGTATGTTTTATTCGGCTAAAGATTAAAATTGTGCGTTTGAAATTGTTGACAATCCTGTAATCTTATGAATTCAGTAACTTAACAAAAGTAGTTTATCGATTAGAACAAACTAAAAGGAAACTTCCACACAATACCCTAATATCTCATACGTTTGGGATTCTGGTAACTATTTCATTTGTTCTAGGAATTTGATATCATCTTTTCCATTAAGTTCGCAATTGGTATTTTCAGCATCTGTACAGTTTACCCCTTTTTAACAGGCACTAGCACTGTAATTAGCTAATAATTTCCATTGCATATATCTCAAGGTTGTTGGAAATTGATCCCATCCCACAATGAGTTCAAGCAGTGACTCCATTGGTGAAACTTATTATGATGTACTGTCTTTGAGAGAAGATGCTAGCTACGACGAAATTCGAGCCAGCTATCGGTCTGCTCTCCTCAATTTCCACCCTGATAAGTTACAAGCTATGTGCCATAAATCTCATCCGGACAACTTTGCGGGAGAAAGATACTTCAAGGTGCAGAAGGCTTGGGAAGTCCTCGGCAGCTCAATGTCCCGTGCATCTTATGACAGAGAGCTCCAAGCTGCCAAAGGGGATGCAATTGGTGCAGAGAGCATAAGCTTAGAGGATATGGTTGTGGAAGATAAAGGTGAACTCGTAGAACTTTTTTATCAGTGCCGTTGTGGGGATTACTTCTTTATTGATTCGGGGGAGTTGGATGAAATGGGATATCCATTGTTGAGGAATGGAAGTAAGGTTTCTTTAAGGACTCTGGATGCTTTGCCTGCTTCCGTTGTTTTACCTTGTGGTTCTTGCTCTTTGAAAGTTCGTTTGCTTATTGATTCAAATTCAAGTGTTTCAATTGATTGAAATCACAATGATTTCTTTTAAGATCCTTGGCGTTTCTTTCTTGAATGATTTCGATGTCTGAGAGAGAGAGCTTATGGCTGTGTTGTGCTCTTGGGTAGGGTTTCCTTGGGTTTTCTTTAAATGCAACTTTGTTGAGTGAAGTTGAACAACTTTCCTAGGCTGGTTGGTTCAATTTTGGACATGTGGATTGTGGGGTTACTATAGGCATCTGAAAGACATCAGCTTGAAGGTCTCTTGTCTATCAACTGAATTATCAAGATCTGAGAGAAGTATAACCATTAATCAGAGTATGATGTATGGCTGAAAAACAATTCCACTTGCTTTAGGTACCATGATATGGGGAAAATGTTGCAGTTTCCGTTTCCAAACTATGACTGCTGAGAAAGATGCAAGGTAATTTCCTTCTTTTGTGGACCAGTGTAATATGATTTAGGATGGGACAAGAGATTTTTTTTCCCCTCGCAGGCTCTCTTGTAATACTGAAGACGCCCAAAATGAAGTACAACTGAAAGGGGGCATGATAAATAAGTCTGGCTGTAATCGAATAGGAATAAGCGATGAACTATTTTAGAATAGGTTTACTTCATTTTCGAGCTAGCATATCAACTAAATGATTGATTTGTTCCTCTTAATGTGCCACCTTACGAGAAAATATGTGACAAATTGCCAAGGAAATTCCCAAACTGGTCCTTTGTTCCCTAGGATTGCTGCTTGTGTTACAAGAGGGATGAATCCTCGATCGTTCTCTCCTTCACTGTCCCTTTGCCTTCAGAAGTAATCTTTCATTTTGAGGAAAATTGGCCTGTCCACATGCCTTCCTAGACGGGTAAAGGAGGCCTTGGGAGGTTGGTGTCTTAAAGGCGAGTTGAATTAAAGGGTTCTCTTGATTCTTTTTGTAATAATGTGCAGTACAATGTCTCGTGGTAGTGTTCTAACCGCCAAAGTTCTGTTGTAATTTTAAAATTATAATTAATCTTAATGCTATTATAGTCATTTACGATTGGAAGACTTTCTTATATAGCTCAAGGTTTTCTCCACTCTATAGGTTATTTACCCAGGGTTCAATTTTTAGTTTTTGTTGATATGTTGATATTTCTGTATGTTCATGGTTTGACATCAATCTAAGGAGTGAGTTGGCATTTTCATTCTATTGGAAAAGTTTTTTTTTTTTCCTTTGATGAAACATTACAAATGTAAATGTGCATAATATGCATTTTAGCTTATTTAAAAAATTGTACAATGTTTATTTATTAACTTAAATATTTATTATTCTATTATAAATTATTCAAAAATATCTAGCATTGACATTTTGTCATTATTATCATTAAAATTGAGATTTCGGTATTTTTCATCTTGACATTGAAAAAATATTAGGTATTTACAAAGGCAATGGACAAGATAACGAAAGACAACTACTAACAAACTTGATAAAAATGACAATGCAAGCATATCACTCAACAAATTAAAATGGGACCAAGCATCATTTTTCTAGAAAAATACGACATAATTAACCAGATTAAAAGGCTTTTGTACCCAACTAAACGTGAAAAGGGAAGTAATATTTTAAGAAATGCCTAATAACGTTCTCAACTTACTTAAGATGAGGTGTCTCAATCCCATCTTGTAGGAATAGATTTTCAGCAGTGTCATCTTTGCCTTTAAGCAGCCGGATCAAGATGCTTTGCCTCACATAACAGGCACGATGGAGAACAAGTCAAATTGGATGGCTATCAATCAATTTTATGACACTGTTAAAGCTTAA

mRNA sequence

ATGCCTCTCCATTTTCACGCCAGCTTCCCGTTGCGTCGCACCACCCCGCCGCCGCCATCTCCGTTGGGCTCAACGCCGCACCCTTTCTTCGCCGATCTTCTCCCCTCCGTTAGTCTCAGTGACTCCATTGGTGAAACTTATTATGATGTACTGTCTTTGAGAGAAGATGCTAGCTACGACGAAATTCGAGCCAGCTATCGGTCTGCTCTCCTCAATTTCCACCCTGATAAGTTACAAGCTATGTGCCATAAATCTCATCCGGACAACTTTGCGGGAGAAAGATACTTCAAGGTGCAGAAGGCTTGGGAAGTCCTCGGCAGCTCAATGTCCCGTGCATCTTATGACAGAGAGCTCCAAGCTGCCAAAGGGGATGCAATTGGTGCAGAGAGCATAAGCTTAGAGGATATGGTTGTGGAAGATAAAGGTGAACTCGTAGAACTTTTTTATCAGTGCCGTTGTGGGGATTACTTCTTTATTGATTCGGGGGAGTTGGATGAAATGGGATATCCATTGTTGAGGAATGGAAGTAAGGTTTCTTTAAGGACTCTGGATGCTTTGCCTGCTTCCGTTGTTTTACCTTGTGGTTCTTGCTCTTTGAAAGTTCGTTTGCTTATTGATTCAAATTCAAGTACCATGATATGGGGAAAATGTTGCAGTTTCCGTTTCCAAACTATGACTGCTGAGAAAGATGCAAGTACAATGTCTCGTGGTAGTGTTCTAACCGCCAAAGTTCTGTTTGTCATCTTTGCCTTTAAGCAGCCGGATCAAGATGCTTTGCCTCACATAACAGGCACGATGGAGAACAAGTCAAATTGGATGGCTATCAATCAATTTTATGACACTGTTAAAGCTTAA

Coding sequence (CDS)

ATGCCTCTCCATTTTCACGCCAGCTTCCCGTTGCGTCGCACCACCCCGCCGCCGCCATCTCCGTTGGGCTCAACGCCGCACCCTTTCTTCGCCGATCTTCTCCCCTCCGTTAGTCTCAGTGACTCCATTGGTGAAACTTATTATGATGTACTGTCTTTGAGAGAAGATGCTAGCTACGACGAAATTCGAGCCAGCTATCGGTCTGCTCTCCTCAATTTCCACCCTGATAAGTTACAAGCTATGTGCCATAAATCTCATCCGGACAACTTTGCGGGAGAAAGATACTTCAAGGTGCAGAAGGCTTGGGAAGTCCTCGGCAGCTCAATGTCCCGTGCATCTTATGACAGAGAGCTCCAAGCTGCCAAAGGGGATGCAATTGGTGCAGAGAGCATAAGCTTAGAGGATATGGTTGTGGAAGATAAAGGTGAACTCGTAGAACTTTTTTATCAGTGCCGTTGTGGGGATTACTTCTTTATTGATTCGGGGGAGTTGGATGAAATGGGATATCCATTGTTGAGGAATGGAAGTAAGGTTTCTTTAAGGACTCTGGATGCTTTGCCTGCTTCCGTTGTTTTACCTTGTGGTTCTTGCTCTTTGAAAGTTCGTTTGCTTATTGATTCAAATTCAAGTACCATGATATGGGGAAAATGTTGCAGTTTCCGTTTCCAAACTATGACTGCTGAGAAAGATGCAAGTACAATGTCTCGTGGTAGTGTTCTAACCGCCAAAGTTCTGTTTGTCATCTTTGCCTTTAAGCAGCCGGATCAAGATGCTTTGCCTCACATAACAGGCACGATGGAGAACAAGTCAAATTGGATGGCTATCAATCAATTTTATGACACTGTTAAAGCTTAA

Protein sequence

MPLHFHASFPLRRTTPPPPSPLGSTPHPFFADLLPSVSLSDSIGETYYDVLSLREDASYDEIRASYRSALLNFHPDKLQAMCHKSHPDNFAGERYFKVQKAWEVLGSSMSRASYDRELQAAKGDAIGAESISLEDMVVEDKGELVELFYQCRCGDYFFIDSGELDEMGYPLLRNGSKVSLRTLDALPASVVLPCGSCSLKVRLLIDSNSSTMIWGKCCSFRFQTMTAEKDASTMSRGSVLTAKVLFVIFAFKQPDQDALPHITGTMENKSNWMAINQFYDTVKA
Homology
BLAST of CaUC08G155270 vs. NCBI nr
Match: KAA0060877.1 (DPH4-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 335.5 bits (859), Expect = 4.5e-88
Identity = 175/210 (83.33%), Postives = 180/210 (85.71%), Query Frame = 0

Query: 1   MPLHFHASFPLRRTTPPPPSPLGSTPHPFFADLLPSV----------SLSDSIGETYYDV 60
           MPLH    FPLR T  PP SP  S PHPFFADLLP +          S S +IGETYYDV
Sbjct: 1   MPLH----FPLRPTIAPPRSPPCSAPHPFFADLLPPLVVGNWTNTMSSSSGAIGETYYDV 60

Query: 61  LSLREDASYDEIRASYRSALLNFHPDKLQAMCHKSHPDNFAGERYFKVQKAWEVLGSSMS 120
           LSLREDAS+DEIRASYRSALLNFHPDKLQAMC KSHPD+  GERYFKVQKAWEVLGS  S
Sbjct: 61  LSLREDASFDEIRASYRSALLNFHPDKLQAMCRKSHPDDIKGERYFKVQKAWEVLGSPKS 120

Query: 121 RASYDRELQAAKGDAIGAESISLEDMVVEDKGELVELFYQCRCGDYFFIDSGELDEMGYP 180
           RASYDRELQAAKGDAIGAESISLEDMVVEDKGE+VELFYQCRCGD FFIDSGELDEMGYP
Sbjct: 121 RASYDRELQAAKGDAIGAESISLEDMVVEDKGEVVELFYQCRCGDCFFIDSGELDEMGYP 180

Query: 181 LLRNGSKVSLRTLDALPASVVLPCGSCSLK 201
           LLRNGSKVSLRTLDALPASVVLPCGSCSLK
Sbjct: 181 LLRNGSKVSLRTLDALPASVVLPCGSCSLK 206

BLAST of CaUC08G155270 vs. NCBI nr
Match: XP_038884890.1 (DPH4 homolog isoform X2 [Benincasa hispida])

HSP 1 Score: 333.6 bits (854), Expect = 1.7e-87
Identity = 167/176 (94.89%), Postives = 170/176 (96.59%), Query Frame = 0

Query: 38  SLSDSIGETYYDVLSLREDASYDEIRASYRSALLNFHPDKLQAMCHKSHPDNFAGERYFK 97
           S S SIGETYYDVLSL+EDASYDEIRASYRSALLNFHPDKLQAMCHKSHPD+ A ERYFK
Sbjct: 3   SSSGSIGETYYDVLSLKEDASYDEIRASYRSALLNFHPDKLQAMCHKSHPDDIALERYFK 62

Query: 98  VQKAWEVLGSSMSRASYDRELQAAKGDAIGAESISLEDMVVEDKGELVELFYQCRCGDYF 157
           VQKAWEVLGSSMSRASYDRELQAAKGDAIGAESISLEDMVVEDKGE+VELFYQCRCGDYF
Sbjct: 63  VQKAWEVLGSSMSRASYDRELQAAKGDAIGAESISLEDMVVEDKGEVVELFYQCRCGDYF 122

Query: 158 FIDSGELDEMGYPLLRNGSKVSLRTLDALPASVVLPCGSCSLKVRLLIDSNSSTMI 214
           FIDSGELDEMGYPLLRNGSKVSLRTLDALPASVVLPCGSCSLKVRLLIDSNSS  I
Sbjct: 123 FIDSGELDEMGYPLLRNGSKVSLRTLDALPASVVLPCGSCSLKVRLLIDSNSSVSI 178

BLAST of CaUC08G155270 vs. NCBI nr
Match: XP_004152732.1 (DPH4 homolog [Cucumis sativus] >KGN62535.1 hypothetical protein Csa_022638 [Cucumis sativus])

HSP 1 Score: 325.9 bits (834), Expect = 3.5e-85
Identity = 161/171 (94.15%), Postives = 166/171 (97.08%), Query Frame = 0

Query: 38  SLSDSIGETYYDVLSLREDASYDEIRASYRSALLNFHPDKLQAMCHKSHPDNFAGERYFK 97
           S+S SIGETYYDVLSLREDAS+DEIRASYRSALLNFHPDKLQAMCHKSHPD+  GERYFK
Sbjct: 3   SISGSIGETYYDVLSLREDASFDEIRASYRSALLNFHPDKLQAMCHKSHPDDIMGERYFK 62

Query: 98  VQKAWEVLGSSMSRASYDRELQAAKGDAIGAESISLEDMVVEDKGELVELFYQCRCGDYF 157
           VQKAWEVLGSS SRASYDRELQAAKGDAIGAESISLEDMVVEDKGE+VEL YQCRCGDYF
Sbjct: 63  VQKAWEVLGSSKSRASYDRELQAAKGDAIGAESISLEDMVVEDKGEVVELLYQCRCGDYF 122

Query: 158 FIDSGELDEMGYPLLRNGSKVSLRTLDALPASVVLPCGSCSLKVRLLIDSN 209
           FIDSGELDEMGYPLLRNGSKVSLRTL+ALPASVVLPCGSCSLKVRLLIDSN
Sbjct: 123 FIDSGELDEMGYPLLRNGSKVSLRTLNALPASVVLPCGSCSLKVRLLIDSN 173

BLAST of CaUC08G155270 vs. NCBI nr
Match: XP_022996470.1 (DPH4 homolog [Cucurbita maxima] >XP_022996471.1 DPH4 homolog [Cucurbita maxima])

HSP 1 Score: 321.2 bits (822), Expect = 8.7e-84
Identity = 160/175 (91.43%), Postives = 164/175 (93.71%), Query Frame = 0

Query: 38  SLSDSIGETYYDVLSLREDASYDEIRASYRSALLNFHPDKLQAMCHKSHPDNFAGERYFK 97
           S S SIGETYYDVLSLREDASYDEIRASYRSALLNFHPDKLQAMCHKSH D+ AGERY K
Sbjct: 3   SSSGSIGETYYDVLSLREDASYDEIRASYRSALLNFHPDKLQAMCHKSHLDDIAGERYLK 62

Query: 98  VQKAWEVLGSSMSRASYDRELQAAKGDAIGAESISLEDMVVEDKGELVELFYQCRCGDYF 157
           VQKAWEVL SSMSRA+YDRELQAAKGDAIGAESI LEDM VEDKGE+VELFYQCRCGDYF
Sbjct: 63  VQKAWEVLSSSMSRAAYDRELQAAKGDAIGAESIRLEDMAVEDKGEVVELFYQCRCGDYF 122

Query: 158 FIDSGELDEMGYPLLRNGSKVSLRTLDALPASVVLPCGSCSLKVRLLIDSNSSTM 213
           FIDSGELDEMGYPLLRNG KVSLRTLD LPASVVLPCGSCSLKVRLLIDSNSS +
Sbjct: 123 FIDSGELDEMGYPLLRNGRKVSLRTLDVLPASVVLPCGSCSLKVRLLIDSNSSVL 177

BLAST of CaUC08G155270 vs. NCBI nr
Match: XP_022962179.1 (DPH4 homolog [Cucurbita moschata] >XP_022962180.1 DPH4 homolog [Cucurbita moschata] >KAG7029347.1 JJJ3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 319.7 bits (818), Expect = 2.5e-83
Identity = 159/175 (90.86%), Postives = 164/175 (93.71%), Query Frame = 0

Query: 38  SLSDSIGETYYDVLSLREDASYDEIRASYRSALLNFHPDKLQAMCHKSHPDNFAGERYFK 97
           S S SIGETYYDVLSLREDASYDEIRASYRSALLNFHPDKLQAMCHKSH D+ AGERY K
Sbjct: 3   SSSGSIGETYYDVLSLREDASYDEIRASYRSALLNFHPDKLQAMCHKSHLDDIAGERYLK 62

Query: 98  VQKAWEVLGSSMSRASYDRELQAAKGDAIGAESISLEDMVVEDKGELVELFYQCRCGDYF 157
           VQKAWEVL SSMSRA+YDRELQAAKGDAIGAESI LEDM VEDKGE+VELFYQCRCGDYF
Sbjct: 63  VQKAWEVLSSSMSRAAYDRELQAAKGDAIGAESIRLEDMAVEDKGEVVELFYQCRCGDYF 122

Query: 158 FIDSGELDEMGYPLLRNGSKVSLRTLDALPASVVLPCGSCSLKVRLLIDSNSSTM 213
           FIDSGELDEMGYPLLRNG KVSLRTLD LPASVVLPCGSCSL+VRLLIDSNSS +
Sbjct: 123 FIDSGELDEMGYPLLRNGRKVSLRTLDVLPASVVLPCGSCSLEVRLLIDSNSSVL 177

BLAST of CaUC08G155270 vs. ExPASy Swiss-Prot
Match: Q54CI5 (DPH4 homolog OS=Dictyostelium discoideum OX=44689 GN=dph4 PE=3 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 2.1e-11
Identity = 46/156 (29.49%), Postives = 76/156 (48.72%), Query Frame = 0

Query: 45  ETYYDVLSLREDASYDEIRASYRSALLNFHPDKL--------------QAMCHKSHPDNF 104
           + YY++L +  DA  +EI+ SYR   L +HPDKL                + + ++ +N 
Sbjct: 14  KNYYEILKVSIDADIEEIKKSYRKLALLYHPDKLNKEENIEENINNFSNCLVNNNNNNNN 73

Query: 105 AGERYFK-VQKAWEVLGSSMSRASYDRELQAAKGDAIG-AESISLEDM-VVEDKGELVEL 164
           +  + F  +Q AWE L   + R  YD  L   K      ++ I L+DM  +E+  E V  
Sbjct: 74  SNTKDFNDIQIAWETLKDDLLRKQYDSLLLEQKRQKYSVSDEIDLDDMEFIEENSEYV-- 133

Query: 165 FYQCRCGDYFFIDSGELDEMGYPLLRNGSKVSLRTL 184
            Y CRCGD++ I   +L E    +  +G  +S++ +
Sbjct: 134 -YPCRCGDHYIITEDQLSEGSDVVCCSGCSLSIKVI 166

BLAST of CaUC08G155270 vs. ExPASy Swiss-Prot
Match: P47138 (Diphthamide biosynthesis protein 4 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=JJJ3 PE=1 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 1.9e-09
Identity = 53/182 (29.12%), Postives = 86/182 (47.25%), Query Frame = 0

Query: 37  VSLSDSIGETYYDVLSLREDASYDEIRASYRSALLNFHPDKLQAMCHKSHPDNFAGERYF 96
           +SL +S+  T+Y++L +  DA+ DEI+ +YR+ LLN HPDKL    H    D  +     
Sbjct: 1   MSLVNSL--THYEILRIPSDATQDEIKKAYRNRLLNTHPDKLSKSIH----DTVSNVTIN 60

Query: 97  KVQKAWEVLGSSMSRASYDR------ELQAAKGDAIGAESISLEDMVV-EDKGELVELFY 156
           K+Q A+++L +  +R  YDR      + Q       G +  SL+D    EDK E +    
Sbjct: 61  KIQDAYKILSNIKTRREYDRLILENYKRQGFHNCGDGLDEFSLDDFSFDEDKLEFMMNCP 120

Query: 157 QCRCGDYFFIDSGELDEMGYPLLRNGSKVSLRTLDALPAS-----VVLPCGSCSLKVRLL 207
           +C+    F      LDE             +  +DA+  S     ++  C +CSL +++ 
Sbjct: 121 RCQFVGGFHFSESLLDE------------CIDNVDAMERSHSGYQLLTQCSACSLWLKVN 164

BLAST of CaUC08G155270 vs. ExPASy Swiss-Prot
Match: Q6FWM1 (Diphthamide biosynthesis protein 4 OS=Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) OX=284593 GN=DPH4 PE=3 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 1.4e-07
Identity = 48/167 (28.74%), Postives = 82/167 (49.10%), Query Frame = 0

Query: 46  TYYDVLSLREDASYDEIRASYRSALLNFHPDKLQAMCHKSHPDNFAGERYFKVQKAWEVL 105
           ++Y+VL +  DAS DEI+ +YR  LL  HPDK + +   S     +G    ++Q+A+ VL
Sbjct: 10  SHYEVLGVPVDASTDEIKLTYRKMLLTLHPDKSKTVPIDSTIKIRSGVSINQIQEAYRVL 69

Query: 106 GSSMSRASYDRELQAAK--------GDAIGAESISLEDMVV-EDKGELVELFYQCRCGDY 165
            +   RA+Y+R L  +         GD  G +  +L++    E+K E V    +C+ G  
Sbjct: 70  SNDQLRAAYNRALNDSNKLAGFNNFGD--GLDEFNLDEFEFNEEKLEYVMTCPRCQSGGG 129

Query: 166 FFIDSGELDEMGYPLLRNGSKVSLRTLDALPASVVLPCGSCSLKVRL 204
           F +    L+E     + +G    L   +     V+  C +CSL +++
Sbjct: 130 FVLSEDMLEE----CIEDG----LTESEEQGYQVLTQCTACSLWLKV 166

BLAST of CaUC08G155270 vs. ExPASy Swiss-Prot
Match: B9KH92 (Chaperone protein DnaJ OS=Anaplasma marginale (strain Florida) OX=320483 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 4.0e-07
Identity = 28/73 (38.36%), Postives = 44/73 (60.27%), Query Frame = 0

Query: 44  GETYYDVLSLREDASYDEIRASYRSALLNFHPDKLQAMCHKSHPDNFAGERYFKVQKAWE 103
           G  YY++L +  +AS +EI+ SYR  +  +HPD       K+  D  A E++ K+ +A+E
Sbjct: 3   GNDYYEILEVSRNASAEEIKKSYRKMVFKYHPD-------KNPGDKKAEEKFKKISEAYE 62

Query: 104 VLGSSMSRASYDR 117
           VL +   RA+YDR
Sbjct: 63  VLSNPEKRAAYDR 68

BLAST of CaUC08G155270 vs. ExPASy Swiss-Prot
Match: Q5P9E0 (Chaperone protein DnaJ OS=Anaplasma marginale (strain St. Maries) OX=234826 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 4.0e-07
Identity = 28/73 (38.36%), Postives = 44/73 (60.27%), Query Frame = 0

Query: 44  GETYYDVLSLREDASYDEIRASYRSALLNFHPDKLQAMCHKSHPDNFAGERYFKVQKAWE 103
           G  YY++L +  +AS +EI+ SYR  +  +HPD       K+  D  A E++ K+ +A+E
Sbjct: 3   GNDYYEILEVSRNASAEEIKKSYRKMVFKYHPD-------KNPGDKKAEEKFKKISEAYE 62

Query: 104 VLGSSMSRASYDR 117
           VL +   RA+YDR
Sbjct: 63  VLSNPEKRAAYDR 68

BLAST of CaUC08G155270 vs. ExPASy TrEMBL
Match: A0A5A7V202 (DPH4-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G00090 PE=3 SV=1)

HSP 1 Score: 335.5 bits (859), Expect = 2.2e-88
Identity = 175/210 (83.33%), Postives = 180/210 (85.71%), Query Frame = 0

Query: 1   MPLHFHASFPLRRTTPPPPSPLGSTPHPFFADLLPSV----------SLSDSIGETYYDV 60
           MPLH    FPLR T  PP SP  S PHPFFADLLP +          S S +IGETYYDV
Sbjct: 1   MPLH----FPLRPTIAPPRSPPCSAPHPFFADLLPPLVVGNWTNTMSSSSGAIGETYYDV 60

Query: 61  LSLREDASYDEIRASYRSALLNFHPDKLQAMCHKSHPDNFAGERYFKVQKAWEVLGSSMS 120
           LSLREDAS+DEIRASYRSALLNFHPDKLQAMC KSHPD+  GERYFKVQKAWEVLGS  S
Sbjct: 61  LSLREDASFDEIRASYRSALLNFHPDKLQAMCRKSHPDDIKGERYFKVQKAWEVLGSPKS 120

Query: 121 RASYDRELQAAKGDAIGAESISLEDMVVEDKGELVELFYQCRCGDYFFIDSGELDEMGYP 180
           RASYDRELQAAKGDAIGAESISLEDMVVEDKGE+VELFYQCRCGD FFIDSGELDEMGYP
Sbjct: 121 RASYDRELQAAKGDAIGAESISLEDMVVEDKGEVVELFYQCRCGDCFFIDSGELDEMGYP 180

Query: 181 LLRNGSKVSLRTLDALPASVVLPCGSCSLK 201
           LLRNGSKVSLRTLDALPASVVLPCGSCSLK
Sbjct: 181 LLRNGSKVSLRTLDALPASVVLPCGSCSLK 206

BLAST of CaUC08G155270 vs. ExPASy TrEMBL
Match: A0A0A0LKX8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G360030 PE=3 SV=1)

HSP 1 Score: 325.9 bits (834), Expect = 1.7e-85
Identity = 161/171 (94.15%), Postives = 166/171 (97.08%), Query Frame = 0

Query: 38  SLSDSIGETYYDVLSLREDASYDEIRASYRSALLNFHPDKLQAMCHKSHPDNFAGERYFK 97
           S+S SIGETYYDVLSLREDAS+DEIRASYRSALLNFHPDKLQAMCHKSHPD+  GERYFK
Sbjct: 3   SISGSIGETYYDVLSLREDASFDEIRASYRSALLNFHPDKLQAMCHKSHPDDIMGERYFK 62

Query: 98  VQKAWEVLGSSMSRASYDRELQAAKGDAIGAESISLEDMVVEDKGELVELFYQCRCGDYF 157
           VQKAWEVLGSS SRASYDRELQAAKGDAIGAESISLEDMVVEDKGE+VEL YQCRCGDYF
Sbjct: 63  VQKAWEVLGSSKSRASYDRELQAAKGDAIGAESISLEDMVVEDKGEVVELLYQCRCGDYF 122

Query: 158 FIDSGELDEMGYPLLRNGSKVSLRTLDALPASVVLPCGSCSLKVRLLIDSN 209
           FIDSGELDEMGYPLLRNGSKVSLRTL+ALPASVVLPCGSCSLKVRLLIDSN
Sbjct: 123 FIDSGELDEMGYPLLRNGSKVSLRTLNALPASVVLPCGSCSLKVRLLIDSN 173

BLAST of CaUC08G155270 vs. ExPASy TrEMBL
Match: A0A6J1K4U3 (DPH4 homolog OS=Cucurbita maxima OX=3661 GN=LOC111491712 PE=3 SV=1)

HSP 1 Score: 321.2 bits (822), Expect = 4.2e-84
Identity = 160/175 (91.43%), Postives = 164/175 (93.71%), Query Frame = 0

Query: 38  SLSDSIGETYYDVLSLREDASYDEIRASYRSALLNFHPDKLQAMCHKSHPDNFAGERYFK 97
           S S SIGETYYDVLSLREDASYDEIRASYRSALLNFHPDKLQAMCHKSH D+ AGERY K
Sbjct: 3   SSSGSIGETYYDVLSLREDASYDEIRASYRSALLNFHPDKLQAMCHKSHLDDIAGERYLK 62

Query: 98  VQKAWEVLGSSMSRASYDRELQAAKGDAIGAESISLEDMVVEDKGELVELFYQCRCGDYF 157
           VQKAWEVL SSMSRA+YDRELQAAKGDAIGAESI LEDM VEDKGE+VELFYQCRCGDYF
Sbjct: 63  VQKAWEVLSSSMSRAAYDRELQAAKGDAIGAESIRLEDMAVEDKGEVVELFYQCRCGDYF 122

Query: 158 FIDSGELDEMGYPLLRNGSKVSLRTLDALPASVVLPCGSCSLKVRLLIDSNSSTM 213
           FIDSGELDEMGYPLLRNG KVSLRTLD LPASVVLPCGSCSLKVRLLIDSNSS +
Sbjct: 123 FIDSGELDEMGYPLLRNGRKVSLRTLDVLPASVVLPCGSCSLKVRLLIDSNSSVL 177

BLAST of CaUC08G155270 vs. ExPASy TrEMBL
Match: A0A6J1HGB5 (DPH4 homolog OS=Cucurbita moschata OX=3662 GN=LOC111462713 PE=3 SV=1)

HSP 1 Score: 319.7 bits (818), Expect = 1.2e-83
Identity = 159/175 (90.86%), Postives = 164/175 (93.71%), Query Frame = 0

Query: 38  SLSDSIGETYYDVLSLREDASYDEIRASYRSALLNFHPDKLQAMCHKSHPDNFAGERYFK 97
           S S SIGETYYDVLSLREDASYDEIRASYRSALLNFHPDKLQAMCHKSH D+ AGERY K
Sbjct: 3   SSSGSIGETYYDVLSLREDASYDEIRASYRSALLNFHPDKLQAMCHKSHLDDIAGERYLK 62

Query: 98  VQKAWEVLGSSMSRASYDRELQAAKGDAIGAESISLEDMVVEDKGELVELFYQCRCGDYF 157
           VQKAWEVL SSMSRA+YDRELQAAKGDAIGAESI LEDM VEDKGE+VELFYQCRCGDYF
Sbjct: 63  VQKAWEVLSSSMSRAAYDRELQAAKGDAIGAESIRLEDMAVEDKGEVVELFYQCRCGDYF 122

Query: 158 FIDSGELDEMGYPLLRNGSKVSLRTLDALPASVVLPCGSCSLKVRLLIDSNSSTM 213
           FIDSGELDEMGYPLLRNG KVSLRTLD LPASVVLPCGSCSL+VRLLIDSNSS +
Sbjct: 123 FIDSGELDEMGYPLLRNGRKVSLRTLDVLPASVVLPCGSCSLEVRLLIDSNSSVL 177

BLAST of CaUC08G155270 vs. ExPASy TrEMBL
Match: A0A1S3BBM0 (DPH4 homolog OS=Cucumis melo OX=3656 GN=LOC103487907 PE=3 SV=1)

HSP 1 Score: 318.2 bits (814), Expect = 3.6e-83
Identity = 159/176 (90.34%), Postives = 165/176 (93.75%), Query Frame = 0

Query: 38  SLSDSIGETYYDVLSLREDASYDEIRASYRSALLNFHPDKLQAMCHKSHPDNFAGERYFK 97
           S S +IGETYYDVLSLREDAS+DEIRASYRSALLNFHPDKLQAMC KSHPD+  GERYFK
Sbjct: 3   SSSGAIGETYYDVLSLREDASFDEIRASYRSALLNFHPDKLQAMCRKSHPDDIKGERYFK 62

Query: 98  VQKAWEVLGSSMSRASYDRELQAAKGDAIGAESISLEDMVVEDKGELVELFYQCRCGDYF 157
           VQKAWEVLGS  SRASYDRELQAAKGDAIGAESISLEDMVVEDKGE+VELFYQCRCGD F
Sbjct: 63  VQKAWEVLGSPKSRASYDRELQAAKGDAIGAESISLEDMVVEDKGEVVELFYQCRCGDCF 122

Query: 158 FIDSGELDEMGYPLLRNGSKVSLRTLDALPASVVLPCGSCSLKVRLLIDSNSSTMI 214
           FIDSGELDEMGYPLLRNGSKVSLRTLDALPASVVLPCGSCSLKV L+ID+NSS  I
Sbjct: 123 FIDSGELDEMGYPLLRNGSKVSLRTLDALPASVVLPCGSCSLKVLLVIDTNSSVSI 178

BLAST of CaUC08G155270 vs. TAIR 10
Match: AT4G10130.1 (DNAJ heat shock N-terminal domain-containing protein )

HSP 1 Score: 188.3 bits (477), Expect = 8.2e-48
Identity = 91/166 (54.82%), Postives = 122/166 (73.49%), Query Frame = 0

Query: 43  IGETYYDVLSLREDASYDEIRASYRSALLNFHPDKLQAMCHKSHPDNFAGERYFKVQKAW 102
           + ETYY++LS++EDASY+EIR SYRSA+L+ HPDKL      S  D    E++ K+QKAW
Sbjct: 8   VHETYYEILSVKEDASYEEIRNSYRSAILHSHPDKLNNTSRSSSDD----EKFLKIQKAW 67

Query: 103 EVLGSSMSRASYDRELQAAKGDAIGAESISLEDMVVEDKGELVELFYQCRCGDYFFIDSG 162
           EVL  +  R  YD +L++++ D I A+ IS+EDM VE  G++++LFYQCRCGDYF +DS 
Sbjct: 68  EVLSDAELRVVYDNDLRSSRHDGITADEISIEDMSVEITGDVIDLFYQCRCGDYFCVDSS 127

Query: 163 ELDEMGYPLLRNGSKVSLRTLDALPASVVLPCGSCSLKVRLLIDSN 209
           EL  MG+ LLR+G  V ++ L A  ASVVLPCGSCSLK R+ +DS+
Sbjct: 128 ELGTMGFALLRDGDFVYVKRLGAFVASVVLPCGSCSLKTRVWVDSD 169

BLAST of CaUC08G155270 vs. TAIR 10
Match: AT1G56300.1 (Chaperone DnaJ-domain superfamily protein )

HSP 1 Score: 50.1 bits (118), Expect = 3.5e-06
Identity = 33/113 (29.20%), Postives = 57/113 (50.44%), Query Frame = 0

Query: 42  SIGETYYDVLSLREDASYDEIRASYRSALLNFHPDKLQAMCHKSHPDNFAGE---RYFKV 101
           ++  +YY +L +R+DAS  +IR +YR   + +HPD+       +     AGE   R+ ++
Sbjct: 9   NVRSSYYTILGIRKDASVSDIRTAYRKLAMKWHPDRY------ARNPGVAGEAKRRFQQI 68

Query: 102 QKAWEVLGSSMSRASYDRELQAAKGDAIGAESISLEDMV-----VEDKGELVE 147
           Q+A+ VL     R+ YD  L     D        +++M+     V+D GE +E
Sbjct: 69  QEAYSVLNDENKRSMYDVGLYDPHEDDDDDFCDFMQEMISMMNNVKDAGESLE 115

BLAST of CaUC08G155270 vs. TAIR 10
Match: AT4G37480.1 (Chaperone DnaJ-domain superfamily protein )

HSP 1 Score: 50.1 bits (118), Expect = 3.5e-06
Identity = 28/74 (37.84%), Postives = 42/74 (56.76%), Query Frame = 0

Query: 45  ETYYDVLSLREDASYDEIRASYRSALLNFHPDKLQAMCHKSHPDNFAGERYFKVQKAWEV 104
           E  YD+L++ E +S  EI+AS+R      HPD +++   K  P N    R+ ++  A+E+
Sbjct: 55  ENAYDILNVSETSSIAEIKASFRRLAKETHPDLIES---KKDPSN--SRRFVQILAAYEI 114

Query: 105 LGSSMSRASYDREL 119
           L  S  RA YDR L
Sbjct: 115 LSDSEKRAHYDRYL 123

BLAST of CaUC08G155270 vs. TAIR 10
Match: AT1G28210.1 (DNAJ heat shock family protein )

HSP 1 Score: 49.7 bits (117), Expect = 4.6e-06
Identity = 29/99 (29.29%), Postives = 53/99 (53.54%), Query Frame = 0

Query: 28  PFFADLLPSVSLSDSIGETYYDVLSLREDASYDEIRASYRSALLNFHPDKLQAMCHKSHP 87
           P F   + +  +++S    YYDVL +   A+ +EI+ S+      FHPD      ++++P
Sbjct: 30  PLFERYIHATGINNSSARNYYDVLGVSPKATREEIKKSFHELAKKFHPD-----TNRNNP 89

Query: 88  DNFAGERYFKVQKAWEVLGSSMSRASYDRELQAAKGDAI 127
              A  ++ ++++A+E LG+S  R  YD+ LQ    D +
Sbjct: 90  S--AKRKFQEIREAYETLGNSERREEYDK-LQYRNSDYV 120

BLAST of CaUC08G155270 vs. TAIR 10
Match: AT1G28210.2 (DNAJ heat shock family protein )

HSP 1 Score: 49.7 bits (117), Expect = 4.6e-06
Identity = 29/99 (29.29%), Postives = 53/99 (53.54%), Query Frame = 0

Query: 28  PFFADLLPSVSLSDSIGETYYDVLSLREDASYDEIRASYRSALLNFHPDKLQAMCHKSHP 87
           P F   + +  +++S    YYDVL +   A+ +EI+ S+      FHPD      ++++P
Sbjct: 30  PLFERYIHATGINNSSARNYYDVLGVSPKATREEIKKSFHELAKKFHPD-----TNRNNP 89

Query: 88  DNFAGERYFKVQKAWEVLGSSMSRASYDRELQAAKGDAI 127
              A  ++ ++++A+E LG+S  R  YD+ LQ    D +
Sbjct: 90  S--AKRKFQEIREAYETLGNSERREEYDK-LQYRNSDYV 120

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAA0060877.14.5e-8883.33DPH4-like protein [Cucumis melo var. makuwa][more]
XP_038884890.11.7e-8794.89DPH4 homolog isoform X2 [Benincasa hispida][more]
XP_004152732.13.5e-8594.15DPH4 homolog [Cucumis sativus] >KGN62535.1 hypothetical protein Csa_022638 [Cucu... [more]
XP_022996470.18.7e-8491.43DPH4 homolog [Cucurbita maxima] >XP_022996471.1 DPH4 homolog [Cucurbita maxima][more]
XP_022962179.12.5e-8390.86DPH4 homolog [Cucurbita moschata] >XP_022962180.1 DPH4 homolog [Cucurbita moscha... [more]
Match NameE-valueIdentityDescription
Q54CI52.1e-1129.49DPH4 homolog OS=Dictyostelium discoideum OX=44689 GN=dph4 PE=3 SV=1[more]
P471381.9e-0929.12Diphthamide biosynthesis protein 4 OS=Saccharomyces cerevisiae (strain ATCC 2045... [more]
Q6FWM11.4e-0728.74Diphthamide biosynthesis protein 4 OS=Candida glabrata (strain ATCC 2001 / CBS 1... [more]
B9KH924.0e-0738.36Chaperone protein DnaJ OS=Anaplasma marginale (strain Florida) OX=320483 GN=dnaJ... [more]
Q5P9E04.0e-0738.36Chaperone protein DnaJ OS=Anaplasma marginale (strain St. Maries) OX=234826 GN=d... [more]
Match NameE-valueIdentityDescription
A0A5A7V2022.2e-8883.33DPH4-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G00... [more]
A0A0A0LKX81.7e-8594.15Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G360030 PE=3 SV=1[more]
A0A6J1K4U34.2e-8491.43DPH4 homolog OS=Cucurbita maxima OX=3661 GN=LOC111491712 PE=3 SV=1[more]
A0A6J1HGB51.2e-8390.86DPH4 homolog OS=Cucurbita moschata OX=3662 GN=LOC111462713 PE=3 SV=1[more]
A0A1S3BBM03.6e-8390.34DPH4 homolog OS=Cucumis melo OX=3656 GN=LOC103487907 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G10130.18.2e-4854.82DNAJ heat shock N-terminal domain-containing protein [more]
AT1G56300.13.5e-0629.20Chaperone DnaJ-domain superfamily protein [more]
AT4G37480.13.5e-0637.84Chaperone DnaJ-domain superfamily protein [more]
AT1G28210.14.6e-0629.29DNAJ heat shock family protein [more]
AT1G28210.24.6e-0629.29DNAJ heat shock family protein [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (USVL246-FR2) v1
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001623DnaJ domainPRINTSPR00625JDOMAINcoord: 90..110
score: 26.34
coord: 110..129
score: 24.62
coord: 48..66
score: 38.0
coord: 66..81
score: 34.71
IPR001623DnaJ domainSMARTSM00271dnaj_3coord: 45..110
e-value: 1.5E-8
score: 44.4
IPR001623DnaJ domainPFAMPF00226DnaJcoord: 46..115
e-value: 3.1E-14
score: 52.8
IPR001623DnaJ domainPROSITEPS50076DNAJ_2coord: 46..118
score: 14.849801
IPR001623DnaJ domainCDDcd06257DnaJcoord: 46..106
e-value: 5.61128E-14
score: 63.3333
IPR036869Chaperone J-domain superfamilyGENE3D1.10.287.110DnaJ domaincoord: 43..122
e-value: 1.2E-16
score: 62.6
IPR036869Chaperone J-domain superfamilySUPERFAMILY46565Chaperone J-domaincoord: 45..118
IPR007872DPH-type metal-binding domainPFAMPF05207zf-CSLcoord: 130..204
e-value: 3.3E-12
score: 45.9
IPR007872DPH-type metal-binding domainPROSITEPS51074DPH_MBcoord: 127..206
score: 14.937167
IPR036671DPH-type metal-binding domain superfamilyGENE3D3.10.660.10DPH Zinc fingercoord: 123..214
e-value: 7.1E-16
score: 59.5
IPR036671DPH-type metal-binding domain superfamilySUPERFAMILY144217CSL zinc fingercoord: 127..207
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..25
NoneNo IPR availablePANTHERPTHR21454:SF31DIPHTHAMIDE BIOSYNTHESIS PROTEIN 4coord: 40..207
IPR044248Diphthamide biosynthesis protein 3/4-likePANTHERPTHR21454DPH3 HOMOLOG-RELATEDcoord: 40..207

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CaUC08G155270.1CaUC08G155270.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0017183 peptidyl-diphthamide biosynthetic process from peptidyl-histidine
biological_process GO:0002098 tRNA wobble uridine modification
cellular_component GO:0005829 cytosol
cellular_component GO:0005634 nucleus
molecular_function GO:0046872 metal ion binding