CaUC08G150960 (gene) Watermelon (USVL246-FR2) v1

BLAST of CaUC08G150960 vs. NCBI nr
Match: XP_008444882.1 (PREDICTED: dihydrofolate reductase-like [Cucumis melo])

HSP 1 Score: 449.5 bits (1155), Expect = 1.9e-122
Identity = 220/251 (87.65%), Postives = 236/251 (94.02%), Query Frame = 0

Query: 5   MTSFSPPLSSPQPLFTPIPEFNFHTTIPIMGTHAKLLTKPRFLCLHGFRTSAAILKKQVG 64
           M+SF P LSS Q L T IP+F+F  T+PIMG+  K+ TKPRFLCLHGFRTSAAILKKQVG
Sbjct: 1   MSSFFPSLSSHQFLST-IPQFHF-PTVPIMGSDQKIFTKPRFLCLHGFRTSAAILKKQVG 60

Query: 65  KWPASVLDQLDLHFLDAPFPAEGKSDVEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIED 124
           KWP SVLDQLDLHFLDAPFPAEGKSDVEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIE+
Sbjct: 61  KWPPSVLDQLDLHFLDAPFPAEGKSDVEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIEN 120

Query: 125 YMLKHGPFDGFLGFSQGAILSAALPGFQAKGIALTKVPKIKFVIIISGAKFRSESVAEKA 184
           YM++HGPFDGFLGFSQGAILSAALPGFQAKGIALTKVPKIKFVII+SGAKFRSESVAEKA
Sbjct: 121 YMIEHGPFDGFLGFSQGAILSAALPGFQAKGIALTKVPKIKFVIIVSGAKFRSESVAEKA 180

Query: 185 YSTLIGCPSLHFLGEEDFLMPHGQKLLESYIDPTVINHPKGHTVPRLDDKALEIMESFIQ 244
           YST IGCPSLHFLGEEDFLMPHG+KLLESYI+PT+I HPKGHT+PRLDDKALE+MESFIQ
Sbjct: 181 YSTPIGCPSLHFLGEEDFLMPHGKKLLESYIEPTIITHPKGHTIPRLDDKALEVMESFIQ 240

Query: 245 RISKTLDETEE 256
           RISK L+ETEE
Sbjct: 241 RISKMLNETEE 249

BLAST of CaUC08G150960 vs. NCBI nr
Match: XP_038885360.1 (dihydrofolate reductase-like [Benincasa hispida])

HSP 1 Score: 446.0 bits (1146), Expect = 2.1e-121
Identity = 213/236 (90.25%), Postives = 224/236 (94.92%), Query Frame = 0

Query: 20  TPIPEFNFHTTIPIMGTHAKLLTKPRFLCLHGFRTSAAILKKQVGKWPASVLDQLDLHFL 79
           +P P  + ++ IPIMGT AK+  KPRFLCLHGFRTSAAILKKQVGKWPASVLDQ DLHFL
Sbjct: 3   SPFPSLHTNSRIPIMGTDAKIHRKPRFLCLHGFRTSAAILKKQVGKWPASVLDQFDLHFL 62

Query: 80  DAPFPAEGKSDVEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFS 139
           DAPFPAEGKSDVEGIFDPPYFEWFQFS EFTEYRNFDECLSFIEDYMLKHGPFDGFLGFS
Sbjct: 63  DAPFPAEGKSDVEGIFDPPYFEWFQFSQEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFS 122

Query: 140 QGAILSAALPGFQAKGIALTKVPKIKFVIIISGAKFRSESVAEKAYSTLIGCPSLHFLGE 199
           QGAILSAALPGFQAKGIALTKVPKIKFVII+SGAKFRSESVA+KAYST IGCPSLHFLGE
Sbjct: 123 QGAILSAALPGFQAKGIALTKVPKIKFVIIVSGAKFRSESVAQKAYSTPIGCPSLHFLGE 182

Query: 200 EDFLMPHGQKLLESYIDPTVINHPKGHTVPRLDDKALEIMESFIQRISKTLDETEE 256
           EDFLMPHGQKLLESY++PTVINHPKGHTVPRLDDK LE+MESFI+RISKTLDETEE
Sbjct: 183 EDFLMPHGQKLLESYVEPTVINHPKGHTVPRLDDKGLEVMESFIKRISKTLDETEE 238

BLAST of CaUC08G150960 vs. NCBI nr
Match: XP_004152614.1 (dihydrofolate reductase [Cucumis sativus])

HSP 1 Score: 445.3 bits (1144), Expect = 3.6e-121
Identity = 219/251 (87.25%), Postives = 230/251 (91.63%), Query Frame = 0

Query: 5   MTSFSPPLSSPQPLFTPIPEFNFHTTIPIMGTHAKLLTKPRFLCLHGFRTSAAILKKQVG 64
           M  F P LSS Q L T IP+F F  TIPIMG+  K  TKPRFLCLHGFRTSAAILKKQVG
Sbjct: 1   MPPFFPSLSSHQSL-TTIPQFRF-PTIPIMGSDQKFFTKPRFLCLHGFRTSAAILKKQVG 60

Query: 65  KWPASVLDQLDLHFLDAPFPAEGKSDVEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIED 124
           KWP SVLDQLDLHFLDAPFPAEGKSDVEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIE+
Sbjct: 61  KWPLSVLDQLDLHFLDAPFPAEGKSDVEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIEN 120

Query: 125 YMLKHGPFDGFLGFSQGAILSAALPGFQAKGIALTKVPKIKFVIIISGAKFRSESVAEKA 184
           YM+KHGPFDGFLGFSQGAILSAALPGFQAKGIALTKVPKIKFVII+SGAKFRSESVAEKA
Sbjct: 121 YMIKHGPFDGFLGFSQGAILSAALPGFQAKGIALTKVPKIKFVIIVSGAKFRSESVAEKA 180

Query: 185 YSTLIGCPSLHFLGEEDFLMPHGQKLLESYIDPTVINHPKGHTVPRLDDKALEIMESFIQ 244
           YST IGCPSLHFLGEEDFLMPHG+KLLESYI+PT+I HPKGHT+PRLDDKALE+MESFI 
Sbjct: 181 YSTPIGCPSLHFLGEEDFLMPHGKKLLESYIEPTIITHPKGHTIPRLDDKALEVMESFIH 240

Query: 245 RISKTLDETEE 256
           RISK L+E EE
Sbjct: 241 RISKILNENEE 249

BLAST of CaUC08G150960 vs. NCBI nr
Match: KAA0065116.1 (dihydrofolate reductase-like [Cucumis melo var. makuwa])

HSP 1 Score: 429.1 bits (1102), Expect = 2.7e-116
Identity = 203/222 (91.44%), Postives = 215/222 (96.85%), Query Frame = 0

Query: 34  MGTHAKLLTKPRFLCLHGFRTSAAILKKQVGKWPASVLDQLDLHFLDAPFPAEGKSDVEG 93
           MG+  K+ TKPRFLCLHGFRTSAAILKKQVGKWP SVLDQLDLHFLDAPFPAEGKSDVEG
Sbjct: 1   MGSDQKIFTKPRFLCLHGFRTSAAILKKQVGKWPPSVLDQLDLHFLDAPFPAEGKSDVEG 60

Query: 94  IFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQGAILSAALPGFQA 153
           IFDPPYFEWFQFSPEFTEYRNFDECLSFIE+YM++HGPFDGFLGFSQGAILSAALPGFQA
Sbjct: 61  IFDPPYFEWFQFSPEFTEYRNFDECLSFIENYMIEHGPFDGFLGFSQGAILSAALPGFQA 120

Query: 154 KGIALTKVPKIKFVIIISGAKFRSESVAEKAYSTLIGCPSLHFLGEEDFLMPHGQKLLES 213
           KGIALTKVPKIKFVII+SGAKFRSESVAEKAYST IGCPSLHFLGEEDFLMPHG+KLLES
Sbjct: 121 KGIALTKVPKIKFVIIVSGAKFRSESVAEKAYSTPIGCPSLHFLGEEDFLMPHGKKLLES 180

Query: 214 YIDPTVINHPKGHTVPRLDDKALEIMESFIQRISKTLDETEE 256
           YI+PT+I HPKGHT+PRLDDKALE+MESFIQRISK L+ETEE
Sbjct: 181 YIEPTIITHPKGHTIPRLDDKALEVMESFIQRISKMLNETEE 222

BLAST of CaUC08G150960 vs. NCBI nr
Match: XP_023545459.1 (dihydrofolate reductase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 427.9 bits (1099), Expect = 6.0e-116
Identity = 209/251 (83.27%), Postives = 225/251 (89.64%), Query Frame = 0

Query: 5   MTSFSPPLSSPQPLFTPIPEFNFHTTIPIMGTHAKLLTKPRFLCLHGFRTSAAILKKQVG 64
           M+S SP L SP   +   PEF+    IPIMGT  ++  KPRFLCLHGFRTS AILKKQVG
Sbjct: 1   MSSLSPLLCSPISKYRS-PEFHLQ-RIPIMGTDGEIRRKPRFLCLHGFRTSGAILKKQVG 60

Query: 65  KWPASVLDQLDLHFLDAPFPAEGKSDVEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIED 124
           KWP SVL+QLDL FLDAPFPAEGKSD+EGIFDPPYFEWFQFSPEFTEYRNFDECLSFIED
Sbjct: 61  KWPDSVLEQLDLDFLDAPFPAEGKSDIEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIED 120

Query: 125 YMLKHGPFDGFLGFSQGAILSAALPGFQAKGIALTKVPKIKFVIIISGAKFRSESVAEKA 184
           YMLKHGPFDGFLGFSQGAILSAALPG QAKGIALTKVPKIKFVII+SGAKFRS+SVAEKA
Sbjct: 121 YMLKHGPFDGFLGFSQGAILSAALPGLQAKGIALTKVPKIKFVIIVSGAKFRSQSVAEKA 180

Query: 185 YSTLIGCPSLHFLGEEDFLMPHGQKLLESYIDPTVINHPKGHTVPRLDDKALEIMESFIQ 244
           YS+ IGCPSLHFLG+EDFLMPHGQKLLESYI+P VINHPKGHT+PRLDDK LE+MESFI+
Sbjct: 181 YSSAIGCPSLHFLGKEDFLMPHGQKLLESYIEPVVINHPKGHTIPRLDDKGLEVMESFIE 240

Query: 245 RISKTLDETEE 256
           +ISK LDE EE
Sbjct: 241 KISKMLDEAEE 249

BLAST of CaUC08G150960 vs. ExPASy Swiss-Prot
Match: P36591 (Dihydrofolate reductase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=dfr1 PE=2 SV=2)

HSP 1 Score: 95.9 bits (237), Expect = 7.0e-19
Identity = 66/209 (31.58%), Postives = 100/209 (47.85%), Query Frame = 0

Query: 41  LTKP-RFLCLHGFRTSAAILKKQVGKWPASVLDQLDLHFLDAPFPAEGKSD--------- 100
           ++KP + LCLHG+  S  +  K++G     +    +LHF   P  A+ ++D         
Sbjct: 1   MSKPLKVLCLHGWIQSGPVFSKKMGSVQKYLSKYAELHFPTGPVVADEEADPNDEEEKKR 60

Query: 101 ---VEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQGAILSAA 160
              + G  +   F WF+       Y ++DE L  I  YM + GPFDG +GFSQGA + A 
Sbjct: 61  LAALGGEQNGGKFGWFEVEDFKNTYGSWDESLECINQYMQEKGPFDGLIGFSQGAGIGAM 120

Query: 161 LPGFQAKG---IALTKVPKIKFVIIISGAKFRSESVA-EKAYSTLIGCPSLHFLGEEDFL 220
           L      G       + P  KFV+ + G  FR+E    +  Y+  +  PSLH  G  D L
Sbjct: 121 LAQMLQPGQPPNPYVQHPPFKFVVFVGG--FRAEKPEFDHFYNPKLTTPSLHIAGTSDTL 180

Query: 221 MP--HGQKLLESYIDPTVINHPKGHTVPR 231
           +P    ++L+E   +  V+ HP  H VP+
Sbjct: 181 VPLARSKQLVERCENAHVLLHPGQHIVPQ 207

BLAST of CaUC08G150960 vs. ExPASy Swiss-Prot
Match: Q7QBJ0 (Esterase AGAP003155 OS=Anopheles gambiae OX=7165 GN=AGAP003155 PE=3 SV=3)

HSP 1 Score: 88.2 bits (217), Expect = 1.5e-16
Identity = 66/224 (29.46%), Postives = 102/224 (45.54%), Query Frame = 0

Query: 43  KPRFLCLHGFRTSAAILKKQVGKWPASVLDQLDLHFLDAPFPAEGKSDVEGIFDPPYFEW 102
           K + L LHG+R +A   K ++G +   +   ++  F+ AP PA     V G  DP    W
Sbjct: 11  KLKVLALHGYRQNADSFKSKLGSFRKMLNKYVEFVFVSAPHPAAPLEAVGGEPDPNQRSW 70

Query: 103 FQFSPEFTEYR---------NFDECLSFIEDYMLKHGPFDGFLGFSQGAILSAALPGFQA 162
           + F+ +   ++          FDE L  +E      G   G LGFSQGA     L    A
Sbjct: 71  W-FNKDDRTFKGTNQGGPAYGFDESLRLVERTWQAEG-CHGLLGFSQGACFVGLLCDLSA 130

Query: 163 KGIALTKVPKIKFVIIISGAKFRSESVAE-KAYSTLIGCPSLHFLGEEDFLMPH--GQKL 222
           +G+   K    +F ++ SG  FRS S+     Y   +  PSLH  GE D ++     + L
Sbjct: 131 RGMTTMKP---QFAVVASG--FRSGSLVHLNYYENKVQIPSLHIFGETDEIITKDMSEAL 190

Query: 223 LESYIDPTVINHPKGHTVPRLDDKALEIMESFIQRISKTLDETE 255
            E+++DP V+ HP GH  P         ++ F  ++ + L+  E
Sbjct: 191 AETFLDPEVVTHPGGHYFPAQASLKETYVDFFRDQLQQHLEAKE 227

BLAST of CaUC08G150960 vs. ExPASy Swiss-Prot
Match: Q0C7C4 (Esterase AAEL000016 OS=Aedes aegypti OX=7159 GN=AAEL000016 PE=3 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 4.7e-15
Identity = 66/226 (29.20%), Postives = 104/226 (46.02%), Query Frame = 0

Query: 42  TKPRFLCLHGFRTSAAILKKQVGKWPASVLDQLDLHFLDAPFPAEGKSDVEGIFDPPYFE 101
           +K + L LHG+R +    K ++G +   +    +L F+ AP  A    D E   +P   +
Sbjct: 23  SKLKILALHGYRQNGDGFKSKLGSFRKFIGKHAELVFVTAPHIAPPLPDSEAGTEPDPAQ 82

Query: 102 ---WFQFSPEFTEYRN-------FDECLSFIEDYMLKHGPFDGFLGFSQGAILSAALPGF 161
              WF       +  N       F++ L  +E  + K     G LGFSQGA     L   
Sbjct: 83  RSWWFNKDDGTFKGTNKNGPAIGFEDSLKLVEK-VWKQEQCCGLLGFSQGACFVGLLCDL 142

Query: 162 QAKGIALTKVPKIKFVIIISGAKFRSESVAE-KAYSTLIGCPSLHFLGEEDFLMPH--GQ 221
            A+G  +T + K +F ++ SG  FRS S+     Y T +  PSLH  GE D ++P     
Sbjct: 143 SARG--MTSI-KPEFAVLSSG--FRSGSLVHLNCYETKVQIPSLHIYGEADEIIPKEMSM 202

Query: 222 KLLESYIDPTVINHPKGHTVPRLDDKALEIMESFIQRISKTLDETE 255
            L +++ DP ++ HP GH +P    +    +E F +R+   L+  E
Sbjct: 203 ALADTFTDPQILTHPGGHFLPAQASQKQTYVEFFRERLQFHLEAQE 242

BLAST of CaUC08G150960 vs. ExPASy Swiss-Prot
Match: Q94AC1 (Rhodanese-like domain-containing protein 6 OS=Arabidopsis thaliana OX=3702 GN=STR6 PE=2 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 1.1e-14
Identity = 66/217 (30.41%), Postives = 96/217 (44.24%), Query Frame = 0

Query: 43  KPRFLCLHGFRTSAAILKKQVGKWPASVLDQLDLHFLDAPF----------PAEGKSD-- 102
           K R LCLHGFR +A+  K + G     + +  +L F+DAP           P  G  +  
Sbjct: 353 KLRILCLHGFRQNASSFKGRTGSLAKKLKNIAELVFIDAPHELQFIYQTATPPSGVCNKK 412

Query: 103 ----VEGIFDPPYFEWF-----QFSP--EFTEYRNFDECLSFIEDYMLKHGPFDGFLGFS 162
               V   FD P    +     QF P    T+   FD+ L++++    + GPFDG LGFS
Sbjct: 413 FAWLVSSDFDKPSETGWTVAQCQFDPLQYQTQTEGFDKSLTYLKTAFEEKGPFDGILGFS 472

Query: 163 QGAILSAALPGFQAKGIALTKVPKIKFVIIISGAKFRSESVAEKAYSTLIGCPSLHFLG- 222
           QGA ++AA+ G Q +   L      +F ++ SG  F    + E      I CPSLH  G 
Sbjct: 473 QGAAMAAAVCGKQEQ---LVGEIDFRFCVLCSG--FTPWPLLEMKEKRSIKCPSLHIFGS 532

Query: 223 ----EEDFLMPHGQKLLESYID--PTVINHPKGHTVP 230
               +   +      L   + D   T++ H  GH +P
Sbjct: 533 QPGKDRQIVTQASSDLAGLFEDGCATIVEHDFGHIIP 564

BLAST of CaUC08G150960 vs. ExPASy Swiss-Prot
Match: Q9VDL1 (Esterase CG5412 OS=Drosophila melanogaster OX=7227 GN=CG5412 PE=2 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 5.2e-14
Identity = 63/228 (27.63%), Postives = 100/228 (43.86%), Query Frame = 0

Query: 39  KLLTKPRFLCLHGFRTSAAILKKQVGKWPASVLDQLDLHFLDAPFPAEG-KSDVEGIFDP 98
           ++  K R LCLHG+R +    K ++G +        +  F+ AP  A+  +S  E +  P
Sbjct: 26  EITEKVRVLCLHGYRQNGEAFKNKLGSFRKFANKYAEFVFITAPHVAKALESAAEPV--P 85

Query: 99  PYFEW--------FQFSPEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQGAILSAALP 158
               W        F+ + +      F E L  +E+     GPF G LGFSQGA     + 
Sbjct: 86  EQRSWWANKDDGSFKGTNKGGPAFGFQESLRCVEEAWRTQGPFQGLLGFSQGACFVGLIC 145

Query: 159 GFQAKGIALTKVPKIKFVIIISGAKFRSESVAE-KAYSTLIGCPSLHFLGEEDFLMPH-- 218
           G   K   LT + + +F ++ SG  F S S+    AY   I  P+LH  G+ D ++P   
Sbjct: 146 GLAKK--KLTSI-RPEFAVLASG--FLSGSLVHMSAYEEAISIPTLHIYGQTDEIIPKEM 205

Query: 219 GQKLLESYIDPTVINHPKGHTVPRLDDKALEIMESFIQRISKTLDETE 255
            + L   + +  V+ H  GH  P    +    +  F  R+ + L+  E
Sbjct: 206 SESLAARFKNAEVLEHSGGHYFPATAQQKQTFINFFQDRLQEYLEHEE 246

BLAST of CaUC08G150960 vs. ExPASy TrEMBL
Match: A0A1S3BC97 (dihydrofolate reductase-like OS=Cucumis melo OX=3656 GN=LOC103488098 PE=4 SV=1)

HSP 1 Score: 449.5 bits (1155), Expect = 9.2e-123
Identity = 220/251 (87.65%), Postives = 236/251 (94.02%), Query Frame = 0

Query: 5   MTSFSPPLSSPQPLFTPIPEFNFHTTIPIMGTHAKLLTKPRFLCLHGFRTSAAILKKQVG 64
           M+SF P LSS Q L T IP+F+F  T+PIMG+  K+ TKPRFLCLHGFRTSAAILKKQVG
Sbjct: 1   MSSFFPSLSSHQFLST-IPQFHF-PTVPIMGSDQKIFTKPRFLCLHGFRTSAAILKKQVG 60

Query: 65  KWPASVLDQLDLHFLDAPFPAEGKSDVEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIED 124
           KWP SVLDQLDLHFLDAPFPAEGKSDVEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIE+
Sbjct: 61  KWPPSVLDQLDLHFLDAPFPAEGKSDVEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIEN 120

Query: 125 YMLKHGPFDGFLGFSQGAILSAALPGFQAKGIALTKVPKIKFVIIISGAKFRSESVAEKA 184
           YM++HGPFDGFLGFSQGAILSAALPGFQAKGIALTKVPKIKFVII+SGAKFRSESVAEKA
Sbjct: 121 YMIEHGPFDGFLGFSQGAILSAALPGFQAKGIALTKVPKIKFVIIVSGAKFRSESVAEKA 180

Query: 185 YSTLIGCPSLHFLGEEDFLMPHGQKLLESYIDPTVINHPKGHTVPRLDDKALEIMESFIQ 244
           YST IGCPSLHFLGEEDFLMPHG+KLLESYI+PT+I HPKGHT+PRLDDKALE+MESFIQ
Sbjct: 181 YSTPIGCPSLHFLGEEDFLMPHGKKLLESYIEPTIITHPKGHTIPRLDDKALEVMESFIQ 240

Query: 245 RISKTLDETEE 256
           RISK L+ETEE
Sbjct: 241 RISKMLNETEE 249

BLAST of CaUC08G150960 vs. ExPASy TrEMBL
Match: A0A0A0LNX4 (FSH1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G369800 PE=4 SV=1)

HSP 1 Score: 445.3 bits (1144), Expect = 1.7e-121
Identity = 219/251 (87.25%), Postives = 230/251 (91.63%), Query Frame = 0

Query: 5   MTSFSPPLSSPQPLFTPIPEFNFHTTIPIMGTHAKLLTKPRFLCLHGFRTSAAILKKQVG 64
           M  F P LSS Q L T IP+F F  TIPIMG+  K  TKPRFLCLHGFRTSAAILKKQVG
Sbjct: 1   MPPFFPSLSSHQSL-TTIPQFRF-PTIPIMGSDQKFFTKPRFLCLHGFRTSAAILKKQVG 60

Query: 65  KWPASVLDQLDLHFLDAPFPAEGKSDVEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIED 124
           KWP SVLDQLDLHFLDAPFPAEGKSDVEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIE+
Sbjct: 61  KWPLSVLDQLDLHFLDAPFPAEGKSDVEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIEN 120

Query: 125 YMLKHGPFDGFLGFSQGAILSAALPGFQAKGIALTKVPKIKFVIIISGAKFRSESVAEKA 184
           YM+KHGPFDGFLGFSQGAILSAALPGFQAKGIALTKVPKIKFVII+SGAKFRSESVAEKA
Sbjct: 121 YMIKHGPFDGFLGFSQGAILSAALPGFQAKGIALTKVPKIKFVIIVSGAKFRSESVAEKA 180

Query: 185 YSTLIGCPSLHFLGEEDFLMPHGQKLLESYIDPTVINHPKGHTVPRLDDKALEIMESFIQ 244
           YST IGCPSLHFLGEEDFLMPHG+KLLESYI+PT+I HPKGHT+PRLDDKALE+MESFI 
Sbjct: 181 YSTPIGCPSLHFLGEEDFLMPHGKKLLESYIEPTIITHPKGHTIPRLDDKALEVMESFIH 240

Query: 245 RISKTLDETEE 256
           RISK L+E EE
Sbjct: 241 RISKILNENEE 249

BLAST of CaUC08G150960 vs. ExPASy TrEMBL
Match: A0A5A7VFL2 (Dihydrofolate reductase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G004510 PE=4 SV=1)

HSP 1 Score: 429.1 bits (1102), Expect = 1.3e-116
Identity = 203/222 (91.44%), Postives = 215/222 (96.85%), Query Frame = 0

Query: 34  MGTHAKLLTKPRFLCLHGFRTSAAILKKQVGKWPASVLDQLDLHFLDAPFPAEGKSDVEG 93
           MG+  K+ TKPRFLCLHGFRTSAAILKKQVGKWP SVLDQLDLHFLDAPFPAEGKSDVEG
Sbjct: 1   MGSDQKIFTKPRFLCLHGFRTSAAILKKQVGKWPPSVLDQLDLHFLDAPFPAEGKSDVEG 60

Query: 94  IFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQGAILSAALPGFQA 153
           IFDPPYFEWFQFSPEFTEYRNFDECLSFIE+YM++HGPFDGFLGFSQGAILSAALPGFQA
Sbjct: 61  IFDPPYFEWFQFSPEFTEYRNFDECLSFIENYMIEHGPFDGFLGFSQGAILSAALPGFQA 120

Query: 154 KGIALTKVPKIKFVIIISGAKFRSESVAEKAYSTLIGCPSLHFLGEEDFLMPHGQKLLES 213
           KGIALTKVPKIKFVII+SGAKFRSESVAEKAYST IGCPSLHFLGEEDFLMPHG+KLLES
Sbjct: 121 KGIALTKVPKIKFVIIVSGAKFRSESVAEKAYSTPIGCPSLHFLGEEDFLMPHGKKLLES 180

Query: 214 YIDPTVINHPKGHTVPRLDDKALEIMESFIQRISKTLDETEE 256
           YI+PT+I HPKGHT+PRLDDKALE+MESFIQRISK L+ETEE
Sbjct: 181 YIEPTIITHPKGHTIPRLDDKALEVMESFIQRISKMLNETEE 222

BLAST of CaUC08G150960 vs. ExPASy TrEMBL
Match: A0A6J1K928 (dihydrofolate reductase-like OS=Cucurbita maxima OX=3661 GN=LOC111492210 PE=4 SV=1)

HSP 1 Score: 425.6 bits (1093), Expect = 1.4e-115
Identity = 208/251 (82.87%), Postives = 225/251 (89.64%), Query Frame = 0

Query: 5   MTSFSPPLSSPQPLFTPIPEFNFHTTIPIMGTHAKLLTKPRFLCLHGFRTSAAILKKQVG 64
           M+S SP L SP    T  PEF+    IPIMGT  ++  KPRFLCLHGFRTS AILKKQVG
Sbjct: 1   MSSLSPLLCSPISK-TRSPEFHLQ-RIPIMGTDGEIRRKPRFLCLHGFRTSGAILKKQVG 60

Query: 65  KWPASVLDQLDLHFLDAPFPAEGKSDVEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIED 124
           KWP SVL+QLDL FLDAPFPAEGKSD+EGIFDPPYFEWFQFSPEFTEYRNFDECLSFIED
Sbjct: 61  KWPDSVLEQLDLDFLDAPFPAEGKSDIEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIED 120

Query: 125 YMLKHGPFDGFLGFSQGAILSAALPGFQAKGIALTKVPKIKFVIIISGAKFRSESVAEKA 184
           YMLKHGPFDGFLGFSQGAILSAALPG QAKG+ALTKVPKIKFVII+SGAKFRS+SVAEKA
Sbjct: 121 YMLKHGPFDGFLGFSQGAILSAALPGLQAKGMALTKVPKIKFVIIVSGAKFRSQSVAEKA 180

Query: 185 YSTLIGCPSLHFLGEEDFLMPHGQKLLESYIDPTVINHPKGHTVPRLDDKALEIMESFIQ 244
           YS+ IGCPSLHFLG+EDFLMPHGQKLLESYI+P VINHPKGHT+PRLDD+ LE+MESFI+
Sbjct: 181 YSSAIGCPSLHFLGKEDFLMPHGQKLLESYIEPVVINHPKGHTIPRLDDRGLEVMESFIE 240

Query: 245 RISKTLDETEE 256
           +ISK LDE EE
Sbjct: 241 KISKMLDEAEE 249

BLAST of CaUC08G150960 vs. ExPASy TrEMBL
Match: A0A6J1HF66 (dihydrofolate reductase-like OS=Cucurbita moschata OX=3662 GN=LOC111462484 PE=4 SV=1)

HSP 1 Score: 424.5 bits (1090), Expect = 3.2e-115
Identity = 207/251 (82.47%), Postives = 225/251 (89.64%), Query Frame = 0

Query: 5   MTSFSPPLSSPQPLFTPIPEFNFHTTIPIMGTHAKLLTKPRFLCLHGFRTSAAILKKQVG 64
           M+S SP L SP    +  PEF+    IPIMGT  ++  KPRFLCLHGFRTS AILKKQVG
Sbjct: 1   MSSLSPLLCSPISK-SRSPEFHLQ-RIPIMGTDGEIRRKPRFLCLHGFRTSGAILKKQVG 60

Query: 65  KWPASVLDQLDLHFLDAPFPAEGKSDVEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIED 124
           KWP SVL+QLDL FLDAPFPAEGKSD+EGIFDPPYFEWFQFSPEFTEYRNFDECLSFIED
Sbjct: 61  KWPDSVLEQLDLDFLDAPFPAEGKSDIEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIED 120

Query: 125 YMLKHGPFDGFLGFSQGAILSAALPGFQAKGIALTKVPKIKFVIIISGAKFRSESVAEKA 184
           YMLKHGPFDGFLGFSQGAILSAALPG QAKGIALTKVPKIKFVII+SGAKFRS+SVAEKA
Sbjct: 121 YMLKHGPFDGFLGFSQGAILSAALPGLQAKGIALTKVPKIKFVIIVSGAKFRSQSVAEKA 180

Query: 185 YSTLIGCPSLHFLGEEDFLMPHGQKLLESYIDPTVINHPKGHTVPRLDDKALEIMESFIQ 244
           YS +IGCPSLHFLG+EDFLMPHGQKLLESYI+P +INHPKGHT+PRLDD+ LE+MESFI+
Sbjct: 181 YSPVIGCPSLHFLGKEDFLMPHGQKLLESYIEPVIINHPKGHTIPRLDDEGLEVMESFIE 240

Query: 245 RISKTLDETEE 256
           +ISK LDE EE
Sbjct: 241 KISKMLDEAEE 249

BLAST of CaUC08G150960 vs. TAIR 10
Match: AT4G24380.1 (INVOLVED IN: 10-formyltetrahydrofolate biosynthetic process, folic acid and derivative biosynthetic process; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Serine hydrolase (InterPro:IPR005645); BEST Arabidopsis thaliana protein match is: alpha/beta-Hydrolases superfamily protein (TAIR:AT5G65400.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 304.7 bits (779), Expect = 7.1e-83
Identity = 143/223 (64.13%), Postives = 177/223 (79.37%), Query Frame = 0

Query: 34  MGTHAK-LLTKPRFLCLHGFRTSAAILKKQVGKWPASVLDQLDLHFLDAPFPAEGKSDVE 93
           MG+  + +  KPRFLCLHGFRTS  I+K Q+ KWP SV+D+LDL FLDAPFP +GKSDVE
Sbjct: 1   MGSEGRSIARKPRFLCLHGFRTSGEIMKIQLHKWPKSVIDRLDLVFLDAPFPCQGKSDVE 60

Query: 94  GIFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQGAILSAALPGFQ 153
           GIFDPPY+EWFQF+ EFTEY NF++CL ++ED M+K GPFDG +GFSQGAILS  LPG Q
Sbjct: 61  GIFDPPYYEWFQFNKEFTEYTNFEKCLEYLEDRMIKLGPFDGLIGFSQGAILSGGLPGLQ 120

Query: 154 AKGIALTKVPKIKFVIIISGAKFRSESVAEKAYSTLIGCPSLHFLGEEDFLMPHGQKLLE 213
           AKGIA  KVPKIKFVIII GAK +S  +AE AYS+ +   SLHFLGE DFL P+G +L+E
Sbjct: 121 AKGIAFQKVPKIKFVIIIGGAKLKSAKLAENAYSSSLETLSLHFLGETDFLKPYGTQLIE 180

Query: 214 SYIDPTVINHPKGHTVPRLDDKALEIMESFIQRISKTLDETEE 256
           SY +P V++HPKGHTVPRLD+K+LE + +FI  +   + E ++
Sbjct: 181 SYKNPVVVHHPKGHTVPRLDEKSLEKVTAFIDTLEHLVMEEDK 223

BLAST of CaUC08G150960 vs. TAIR 10
Match: AT5G65400.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 276.2 bits (705), Expect = 2.7e-74
Identity = 129/214 (60.28%), Postives = 161/214 (75.23%), Query Frame = 0

Query: 44  PRFLCLHGFRTSAAILKKQVGKWPASVLDQLDLHFLDAPFPAEGKSDVEGIFDPPYFEWF 103
           PR LCLHGFRTS  IL+  +GKWP ++L  LDL FLDAPFPA GKSDVE  FDPPY+EW+
Sbjct: 30  PRILCLHGFRTSGRILQAGIGKWPDTILRDLDLDFLDAPFPATGKSDVERFFDPPYYEWY 89

Query: 104 QFSPEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQGAILSAALPGFQAKGIALTKVPK 163
           Q +  F EYRNF+ECL++IEDYM+K+GPFDG LGFSQGA L+AA+PG Q +G ALTKVPK
Sbjct: 90  QANKGFKEYRNFEECLAYIEDYMIKNGPFDGLLGFSQGAFLTAAIPGMQEQGSALTKVPK 149

Query: 164 IKFVIIISGAK-----FRSESVAEKAYSTLIGCPSLHFLGEEDFLMPHGQKLLESYIDPT 223
           +KF++IISGAK     F     A  A+S+ + CPSLHF+GE DFL   G+ L+ES+++P 
Sbjct: 150 VKFLVIISGAKIPGLMFGEPKAAVNAFSSPVRCPSLHFIGERDFLKIEGEVLVESFVEPV 209

Query: 224 VINHPKGHTVPRLDDKALEIMESFIQRISKTLDE 253
           VI+H  GH +P+LD KA E M SF Q I + L +
Sbjct: 210 VIHHSGGHIIPKLDTKAEETMLSFFQSIRQMLSD 243

BLAST of CaUC08G150960 vs. TAIR 10
Match: AT4G24380.2 (CONTAINS InterPro DOMAIN/s: Serine hydrolase (InterPro:IPR005645); BEST Arabidopsis thaliana protein match is: alpha/beta-Hydrolases superfamily protein (TAIR:AT5G65400.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink). )

HSP 1 Score: 241.5 bits (615), Expect = 7.4e-64
Identity = 114/166 (68.67%), Postives = 134/166 (80.72%), Query Frame = 0

Query: 34  MGTHAK-LLTKPRFLCLHGFRTSAAILKKQVGKWPASVLDQLDLHFLDAPFPAEGKSDVE 93
           MG+  + +  KPRFLCLHGFRTS  I+K Q+ KWP SV+D+LDL FLDAPFP +GKSDVE
Sbjct: 1   MGSEGRSIARKPRFLCLHGFRTSGEIMKIQLHKWPKSVIDRLDLVFLDAPFPCQGKSDVE 60

Query: 94  GIFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQGAILSAALPGFQ 153
           GIFDPPY+EWFQF+ EFTEY NF++CL ++ED M+K GPFDG +GFSQGAILS  LPG Q
Sbjct: 61  GIFDPPYYEWFQFNKEFTEYTNFEKCLEYLEDRMIKLGPFDGLIGFSQGAILSGGLPGLQ 120

Query: 154 AKGIALTKVPKIKFVIIISGAKFRSESVAEKAYSTLIGCPSLHFLG 199
           AKGIA  KVPKIKFVIII GAK +S  +AE AYS+ +   SLHFLG
Sbjct: 121 AKGIAFQKVPKIKFVIIIGGAKLKSAKLAENAYSSSLETLSLHFLG 166

BLAST of CaUC08G150960 vs. TAIR 10
Match: AT1G09280.1 (CONTAINS InterPro DOMAIN/s: Rhodanese-like (InterPro:IPR001763), Serine hydrolase (InterPro:IPR005645); BEST Arabidopsis thaliana protein match is: Rhodanese/Cell cycle control phosphatase superfamily protein (TAIR:AT2G40760.1); Has 5925 Blast hits to 5912 proteins in 1592 species: Archae - 0; Bacteria - 2946; Metazoa - 156; Fungi - 408; Plants - 229; Viruses - 0; Other Eukaryotes - 2186 (source: NCBI BLink). )

HSP 1 Score: 82.0 bits (201), Expect = 7.5e-16
Identity = 66/217 (30.41%), Postives = 96/217 (44.24%), Query Frame = 0

Query: 43  KPRFLCLHGFRTSAAILKKQVGKWPASVLDQLDLHFLDAPF----------PAEGKSD-- 102
           K R LCLHGFR +A+  K + G     + +  +L F+DAP           P  G  +  
Sbjct: 353 KLRILCLHGFRQNASSFKGRTGSLAKKLKNIAELVFIDAPHELQFIYQTATPPSGVCNKK 412

Query: 103 ----VEGIFDPPYFEWF-----QFSP--EFTEYRNFDECLSFIEDYMLKHGPFDGFLGFS 162
               V   FD P    +     QF P    T+   FD+ L++++    + GPFDG LGFS
Sbjct: 413 FAWLVSSDFDKPSETGWTVAQCQFDPLQYQTQTEGFDKSLTYLKTAFEEKGPFDGILGFS 472

Query: 163 QGAILSAALPGFQAKGIALTKVPKIKFVIIISGAKFRSESVAEKAYSTLIGCPSLHFLG- 222
           QGA ++AA+ G Q +   L      +F ++ SG  F    + E      I CPSLH  G 
Sbjct: 473 QGAAMAAAVCGKQEQ---LVGEIDFRFCVLCSG--FTPWPLLEMKEKRSIKCPSLHIFGS 532

Query: 223 ----EEDFLMPHGQKLLESYID--PTVINHPKGHTVP 230
               +   +      L   + D   T++ H  GH +P
Sbjct: 533 QPGKDRQIVTQASSDLAGLFEDGCATIVEHDFGHIIP 564