CaUC07G136690 (gene) Watermelon (USVL246-FR2) v1

Overview
NameCaUC07G136690
Typegene
OrganismCitrullus amarus (Watermelon (USVL246-FR2) v1)
DescriptionProtein NUCLEAR FUSION DEFECTIVE 4
LocationCiama_Chr07: 26857454 .. 26861173 (+)
RNA-Seq ExpressionCaUC07G136690
SyntenyCaUC07G136690
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCGAAGCTGGTTTTGAAAGGGGGGAGTAGGCCTCCATGGGTTGGCTTAGCTGCTGCTGTTTGGGTTCAAATAGCTGGTGGTTCTTCTTATAACTTTTCTCTTTATTCACACTTGCTTAAATCTGTTCTGGGTTTGAATCAGCAGCAGCTTACTGTGTTGGGAGTGGCTAATGACATTGGAGAGAGTATGGGTTTGGTTCCTGGCCTTGCCTGTAACAGATTTCCTCCATGGGTTATACTTTTGTTTGGTGCTTTCTGTTGCTTTCTGGGTTATGGGGCTATTTGGCTTGCTGTTAGCAGGACTGTCCCCAATCTGCCTTATTGGCTGGTGAGTTTAATGTTTATCTTTTCCCTTTGTGAAGATCTTTCTCTCATTTTTCTATCTGTTCTACTGGCTGCTGATTTTCATGAACTTTTGAGGTTTTTTCTTTGTTGGTTGTTGTTTGCTGTTCTTGATGAATGGGACAATTTGTTTTTTTTGTTACTATAAGATTGCCTATGATCTAAGTCTTGATGTTTCTCTATTAATGAAGTTGTTTTTTTGGTTTCTGGGAGATCTTGAATCAAAGCTGCTTTGTAACTAGTTATAATTGGATTGATAACTGATAAGCTAAATGCTGAGGGTTTTGATACAGAAGTTACTTTGTAAGTTTTGATGCAAAACTTATTATATTAATAATGCAAAGGCTTGATAAGAGATCCCCGCAATCCACCTAAATGAGATTATACGAAAAAGAGCTTTTCAATTAGTAAAAAAGAAAGAAAAAAAAAATCCTATTATTTCCTTCTACCCATTACTCCAATTAGTTAGCCAAGAAGTAGACTAAGGAAGTTTAGCTTTTGTTTCGGTTGGATGCGGGGATGTGCAGTGGCGGGATCAAACATAAATTATCTTACACTGAGGTCGACCGAGGTTTGATCGAGTTAAGCAATCATGATATACGAGAATTTCCTTGTGGACAAGATTGTCTATCCGAATCTTTGCAACTACGTGAACCCTGTTAAAGTTCAATGGAGTGAACATACAACATTGGGTAACTAATGAGGAAAATTTGTCAACAATGTTGACATTATTTCATTTCCTTTATCCATGGTCAAAGTTTTTCATCTTTAGCACAGAAACAAGAGATGGATGATATGACTGTTTAATAGGTTTAGGGTCAAACTTTGAGCAACTTTAGCAGAATTAGTTATTTCGTTGGCATTTCTCTATATTAAAATGACAGCCATTATTTCTTTTTTGAATATTTGAATGTATATGATTAAAATAATGATGTTATCTATTCATAGCTTCTTGTCCATTTTGAAACTAAAGGTAATTGAGATCTTCAGATGATCTAAATGAAACTAAGCAAGTATATTGATGCCAATATGCTCATGCAATGACTAAGAGGTCAAGGGTTCAATCCATGATGGCCACCTACCTAGGAATTAATTTCTTATGAGTTTCCTTGACACCCAAATGTTGTAGGGTCAGGCAAGTTGTTCTGTGAGATTAGTTGAGGTGCGCGTAAGCTGGCCCGAACACTCACGGATATATATATATATATATATATAAAGCTCATGCCATCTCATGTAACTCACAAGCACAATAAGTCAGATGAAACTTTATATGTATTGAATGGATAAGGTTGTAGTTGATTACAAGATGAATCCTACTATGGTATTCAAGCGACTTTGGAACTCCGTCCTACTTGATTCCCAAGTTCCCTTCATCTACTCTCGATCATACTCTCCTACTCCCAACCGATTTTCCATTTTGTGCCATTCTATTATCTACTAACTCTACTACACACCTTCAATTACATTTTCATATCTCCCATGGTTGATTGATAATTTTCCTAACAGTTTTACCCACCTAGCATAGGTAAAGCAGAAAGGAGGCCTAACATATATGTTAAACGTAGAAAATGGATGGGATATGACTGCTTACATATTCATCCATTTGCAAAACTTAATTTCCTTCACTTTTTCGTAGTCTTTGCAACATTTCTATTAGCTTTGCTTCTTCATCTTGTATTCTTTCATTTCTTCTCAATTAAAGGTTGGTTATTCATTAAAAAAAGAAAAAAAAAATATAGGTCATGTGCTTTAAACATTGTATTCTGAATGTCATGGCAGCTATGGATTACACATTGTATTGCTACGAATAGCAATGCATGGTTTGGAACAGCTGTGCTCGTAACCAACATGAGGAATTTCCCTCTTAGCAGAGGTACTGTTGCTGGCATTCTTAAAGGTTATGTTGGGCTTAGTGCTGCTGTATACACAGTGATATACAGTATGGTGCTGAAAAAGTCTGCTTTGAATTTACTGTTGTTTCTTGCGATCGGGATACCCATTTTGTGCTTAGCCTTGATGTACTTTGTTCGACCTTGTACTCCGGCTTCATCAGAGGATCCATCTGAGCGTGCTCATTTTCTTTTCACACAAGCTGCTTGTGTTTTGCTAGCTGTTTTTTTAGTCTCGACCACAATTCTAGATGCAACAACAACCCCTAGTGACGCCGTTGGATACACCTTAGTTGCCATAATGGTTATCCTTTTAATGTCTCCTCTTGCAGTTCCCATAAAAATGACCATTTGTGCAAAATCCAAAAAACTTGGCCCTCGAGTTGATTCTGCAGAACCTTTGGCTTCTGGAGAAAGCGATTCTTCCCAAATTGAACCTCTATTGACACCATCTTCCTCAGCTACAAATCTTGGAAGTTTCTGCGAGAATGATGATTCATCAGATGTAGAAACACTTCTTGCTGTGGGAGAGGGAGCGATTCATAAGAAGAAGAGAAGACCTAAAAGAGGGGAAGATTTCAAGCTCCGTGAAGCCGTAATCAAAGCTGATTTCTGGCTGCTTTGGTTCTTGTACTTCCTCGGGGTCGGTCCAGGAATAACAATTCTCAACAATTTGTCTCAAATTGGGATTTCATTAGGTGTTAATGACACAACATTGCTGTTGGCTCTTTTCAGCTTTTGCAATTTTGTCGGTCGTCTAGGCTCTGGTGTTATTTCCGAACACTTTGTCAGGTAAATACTTATACAGAACAGATCACTGAATTACTAATTTGTTCCAACTTTTGTCGATAAGGCTTCAAAATCTTCAAATTCAACTATTGCAGAGTCATTTCAGTGTTAAACTGGAGAAATTTTCCAAATTACTTTGATTTTATATTCTGAGTATCTAGTGATTTTCTCATGAAGTTGCTCAATCTACCAAATGCTCATCGGTTCGGTTTGGTATTCATTGGTCGTGCAGGTCAAGAATGATACCTCGCTCACTGTGGATGATGTTTGCACTAATACTCATGGCTATAGCATTCCTCCTGTATGCATCTGCTCTCACGGGTACTCTCTACGTCGCCACTGGTTTAACTGGAATCAGCTACGGAGTTCTGTACTCTATGATGGTCCCTCTCGCATCTGAGATTTTCGGTCTAAAGAATTTTGGAGTAATCTTCAACTTCATGCAGCTGGGGAATCCAATAGGAGCAGTCCTTTTCTCAGTTCTACTCGCCAGTACTCTATACGACAACGAAGCTGCTAAGCAAGGTTCGATAACCTGCATAGGCCAACAGTGCTTCAGGACGACTTTCTTCATCCTTTCGGGCGTTGCTGGGTTAGGTTCGATTGTGAGCATGATTCTGACTGTTAGATTAAGGCCAGTCTATCAAATGCTATACTCCGGTGGCTCGTTCCGCTTGCCTCAAAGTTCAGGTCATTGA

mRNA sequence

ATGCCGAAGCTGGTTTTGAAAGGGGGGAGTAGGCCTCCATGGGTTGGCTTAGCTGCTGCTGTTTGGGTTCAAATAGCTGGTGGTTCTTCTTATAACTTTTCTCTTTATTCACACTTGCTTAAATCTGTTCTGGGTTTGAATCAGCAGCAGCTTACTGTGTTGGGAGTGGCTAATGACATTGGAGAGAGTATGGGTTTGGTTCCTGGCCTTGCCTGTAACAGATTTCCTCCATGGGTTATACTTTTGTTTGGTGCTTTCTGTTGCTTTCTGGGTTATGGGGCTATTTGGCTTGCTGTTAGCAGGACTGTCCCCAATCTGCCTTATTGGCTGGTGAGTTTAATGTTTATCTTTTCCCTTTGTGAAGATCTTTCTCTCATTTTTCTATCTGTTCTACTGGCTGCTGATTTTCATGAACTTTTGAGCAATGCATGGTTTGGAACAGCTGTGCTCGTAACCAACATGAGGAATTTCCCTCTTAGCAGAGGTACTGTTGCTGGCATTCTTAAAGGTTATGTTGGGCTTAGTGCTGCTGTATACACAGTGATATACAGTATGGTGCTGAAAAAGTCTGCTTTGAATTTACTGTTGTTTCTTGCGATCGGGATACCCATTTTGTGCTTAGCCTTGATGTACTTTGTTCGACCTTGTACTCCGGCTTCATCAGAGGATCCATCTGAGCGTGCTCATTTTCTTTTCACACAAGCTGCTTGTGTTTTGCTAGCTGTTTTTTTAGTCTCGACCACAATTCTAGATGCAACAACAACCCCTAGTGACGCCGTTGGATACACCTTAGTTGCCATAATGGTTATCCTTTTAATGTCTCCTCTTGCAGTTCCCATAAAAATGACCATTTGTGCAAAATCCAAAAAACTTGGCCCTCGAGTTGATTCTGCAGAACCTTTGGCTTCTGGAGAAAGCGATTCTTCCCAAATTGAACCTCTATTGACACCATCTTCCTCAGCTACAAATCTTGGAAGTTTCTGCGAGAATGATGATTCATCAGATGTAGAAACACTTCTTGCTGTGGGAGAGGGAGCGATTCATAAGAAGAAGAGAAGACCTAAAAGAGGGGAAGATTTCAAGCTCCGTGAAGCCGTAATCAAAGCTGATTTCTGGCTGCTTTGGTTCTTGTACTTCCTCGGGGTCGGTCCAGGAATAACAATTCTCAACAATTTGTCTCAAATTGGGATTTCATTAGGTGTTAATGACACAACATTGCTGTTGGCTCTTTTCAGCTTTTGCAATTTTGTCGGTCGTCTAGGCTCTGGTGTTATTTCCGAACACTTTGTCAGGTCAAGAATGATACCTCGCTCACTGTGGATGATGTTTGCACTAATACTCATGGCTATAGCATTCCTCCTGTATGCATCTGCTCTCACGGGTACTCTCTACGTCGCCACTGGTTTAACTGGAATCAGCTACGGAGTTCTGTACTCTATGATGGTCCCTCTCGCATCTGAGATTTTCGGTCTAAAGAATTTTGGAGTAATCTTCAACTTCATGCAGCTGGGGAATCCAATAGGAGCAGTCCTTTTCTCAGTTCTACTCGCCAGTACTCTATACGACAACGAAGCTGCTAAGCAAGGTTCGATAACCTGCATAGGCCAACAGTGCTTCAGGACGACTTTCTTCATCCTTTCGGGCGTTGCTGGGTTAGGTTCGATTGTGAGCATGATTCTGACTGTTAGATTAAGGCCAGTCTATCAAATGCTATACTCCGGTGGCTCGTTCCGCTTGCCTCAAAGTTCAGGTCATTGA

Coding sequence (CDS)

ATGCCGAAGCTGGTTTTGAAAGGGGGGAGTAGGCCTCCATGGGTTGGCTTAGCTGCTGCTGTTTGGGTTCAAATAGCTGGTGGTTCTTCTTATAACTTTTCTCTTTATTCACACTTGCTTAAATCTGTTCTGGGTTTGAATCAGCAGCAGCTTACTGTGTTGGGAGTGGCTAATGACATTGGAGAGAGTATGGGTTTGGTTCCTGGCCTTGCCTGTAACAGATTTCCTCCATGGGTTATACTTTTGTTTGGTGCTTTCTGTTGCTTTCTGGGTTATGGGGCTATTTGGCTTGCTGTTAGCAGGACTGTCCCCAATCTGCCTTATTGGCTGGTGAGTTTAATGTTTATCTTTTCCCTTTGTGAAGATCTTTCTCTCATTTTTCTATCTGTTCTACTGGCTGCTGATTTTCATGAACTTTTGAGCAATGCATGGTTTGGAACAGCTGTGCTCGTAACCAACATGAGGAATTTCCCTCTTAGCAGAGGTACTGTTGCTGGCATTCTTAAAGGTTATGTTGGGCTTAGTGCTGCTGTATACACAGTGATATACAGTATGGTGCTGAAAAAGTCTGCTTTGAATTTACTGTTGTTTCTTGCGATCGGGATACCCATTTTGTGCTTAGCCTTGATGTACTTTGTTCGACCTTGTACTCCGGCTTCATCAGAGGATCCATCTGAGCGTGCTCATTTTCTTTTCACACAAGCTGCTTGTGTTTTGCTAGCTGTTTTTTTAGTCTCGACCACAATTCTAGATGCAACAACAACCCCTAGTGACGCCGTTGGATACACCTTAGTTGCCATAATGGTTATCCTTTTAATGTCTCCTCTTGCAGTTCCCATAAAAATGACCATTTGTGCAAAATCCAAAAAACTTGGCCCTCGAGTTGATTCTGCAGAACCTTTGGCTTCTGGAGAAAGCGATTCTTCCCAAATTGAACCTCTATTGACACCATCTTCCTCAGCTACAAATCTTGGAAGTTTCTGCGAGAATGATGATTCATCAGATGTAGAAACACTTCTTGCTGTGGGAGAGGGAGCGATTCATAAGAAGAAGAGAAGACCTAAAAGAGGGGAAGATTTCAAGCTCCGTGAAGCCGTAATCAAAGCTGATTTCTGGCTGCTTTGGTTCTTGTACTTCCTCGGGGTCGGTCCAGGAATAACAATTCTCAACAATTTGTCTCAAATTGGGATTTCATTAGGTGTTAATGACACAACATTGCTGTTGGCTCTTTTCAGCTTTTGCAATTTTGTCGGTCGTCTAGGCTCTGGTGTTATTTCCGAACACTTTGTCAGGTCAAGAATGATACCTCGCTCACTGTGGATGATGTTTGCACTAATACTCATGGCTATAGCATTCCTCCTGTATGCATCTGCTCTCACGGGTACTCTCTACGTCGCCACTGGTTTAACTGGAATCAGCTACGGAGTTCTGTACTCTATGATGGTCCCTCTCGCATCTGAGATTTTCGGTCTAAAGAATTTTGGAGTAATCTTCAACTTCATGCAGCTGGGGAATCCAATAGGAGCAGTCCTTTTCTCAGTTCTACTCGCCAGTACTCTATACGACAACGAAGCTGCTAAGCAAGGTTCGATAACCTGCATAGGCCAACAGTGCTTCAGGACGACTTTCTTCATCCTTTCGGGCGTTGCTGGGTTAGGTTCGATTGTGAGCATGATTCTGACTGTTAGATTAAGGCCAGTCTATCAAATGCTATACTCCGGTGGCTCGTTCCGCTTGCCTCAAAGTTCAGGTCATTGA

Protein sequence

MPKLVLKGGSRPPWVGLAAAVWVQIAGGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDIGESMGLVPGLACNRFPPWVILLFGAFCCFLGYGAIWLAVSRTVPNLPYWLVSLMFIFSLCEDLSLIFLSVLLAADFHELLSNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYTVIYSMVLKKSALNLLLFLAIGIPILCLALMYFVRPCTPASSEDPSERAHFLFTQAACVLLAVFLVSTTILDATTTPSDAVGYTLVAIMVILLMSPLAVPIKMTICAKSKKLGPRVDSAEPLASGESDSSQIEPLLTPSSSATNLGSFCENDDSSDVETLLAVGEGAIHKKKRRPKRGEDFKLREAVIKADFWLLWFLYFLGVGPGITILNNLSQIGISLGVNDTTLLLALFSFCNFVGRLGSGVISEHFVRSRMIPRSLWMMFALILMAIAFLLYASALTGTLYVATGLTGISYGVLYSMMVPLASEIFGLKNFGVIFNFMQLGNPIGAVLFSVLLASTLYDNEAAKQGSITCIGQQCFRTTFFILSGVAGLGSIVSMILTVRLRPVYQMLYSGGSFRLPQSSGH
Homology
BLAST of CaUC07G136690 vs. NCBI nr
Match: XP_038896285.1 (protein NUCLEAR FUSION DEFECTIVE 4 isoform X1 [Benincasa hispida])

HSP 1 Score: 1018.5 bits (2632), Expect = 2.4e-293
Identity = 537/585 (91.79%), Postives = 552/585 (94.36%), Query Frame = 0

Query: 1   MPKLVLKGGSRPPWVGLAAAVWVQIAGGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI 60
           MPKLVLKGGSRPPWVGLAAAVWVQIA GSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI
Sbjct: 1   MPKLVLKGGSRPPWVGLAAAVWVQIAAGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI 60

Query: 61  GESMGLVPGLACNRFPPWVILLFGAFCCFLGYGAIWLAVSRTVPNLPYWLVSLMFIFSLC 120
           GESMGL+PGLACNRFPPWVILLFGAFCCF+GYGAIWLAVSRTVPNLPYW           
Sbjct: 61  GESMGLIPGLACNRFPPWVILLFGAFCCFVGYGAIWLAVSRTVPNLPYW----------- 120

Query: 121 EDLSLIFLSVLLAADFHELLSNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYT 180
               L++L+  +A +     SNAWFGTAVLVTNMRNFPLSRG+VAGILKGYVGLSAAVYT
Sbjct: 121 ----LLWLTHCIATN-----SNAWFGTAVLVTNMRNFPLSRGSVAGILKGYVGLSAAVYT 180

Query: 181 VIYSMVLKKSALNLLLFLAIGIPILCLALMYFVRPCTPASSEDPSERAHFLFTQAACVLL 240
           VIYSMVL+KSALNLLLFLAIGIPILCLALMYFVRPCTPASSEDPSER+HFLFTQA+CVLL
Sbjct: 181 VIYSMVLRKSALNLLLFLAIGIPILCLALMYFVRPCTPASSEDPSERSHFLFTQASCVLL 240

Query: 241 AVFLVSTTILDATTTPSDAVGYTLVAIMVILLMSPLAVPIKMTICAKSKKLGPRVDSAEP 300
           AVFLVSTTI+DATTTPSDAV YTLVAIMVI LMSPLAVPIKMTICAK+KKLG RVDS EP
Sbjct: 241 AVFLVSTTIVDATTTPSDAVAYTLVAIMVIFLMSPLAVPIKMTICAKTKKLGSRVDSVEP 300

Query: 301 LASGESDSSQIEPLLTPSSSATNLGSFCENDDSSDVETLLAVGEGAIHKKKRRPKRGEDF 360
           LASGESDSSQIEPLLTPSSSATNLGSF ENDD+SDVETLLAVGEGAIHKKKRRPKRGEDF
Sbjct: 301 LASGESDSSQIEPLLTPSSSATNLGSFYENDDASDVETLLAVGEGAIHKKKRRPKRGEDF 360

Query: 361 KLREAVIKADFWLLWFLYFLGVGPGITILNNLSQIGISLGVNDTTLLLALFSFCNFVGRL 420
           KLREAVIKADFWLLWFLYF GVGPGIT+LNNLSQIGISLGVNDTTLLLALFSFCNFVGRL
Sbjct: 361 KLREAVIKADFWLLWFLYFFGVGPGITVLNNLSQIGISLGVNDTTLLLALFSFCNFVGRL 420

Query: 421 GSGVISEHFVRSRMIPRSLWMMFALILMAIAFLLYASALTGTLYVATGLTGISYGVLYSM 480
           GSGVISEHFVRSRMIPRSLWMMFALILMAIAFLLYASALTGTLYVATGL GISYGVLYSM
Sbjct: 421 GSGVISEHFVRSRMIPRSLWMMFALILMAIAFLLYASALTGTLYVATGLIGISYGVLYSM 480

Query: 481 MVPLASEIFGLKNFGVIFNFMQLGNPIGAVLFSVLLASTLYDNEAAKQGSITCIGQQCFR 540
           MVPLASEIFGLKNFGVIFNFMQLGNPIGAVLFSVLLASTLYD EAAKQGSITCIGQQCFR
Sbjct: 481 MVPLASEIFGLKNFGVIFNFMQLGNPIGAVLFSVLLASTLYDKEAAKQGSITCIGQQCFR 540

Query: 541 TTFFILSGVAGLGSIVSMILTVRLRPVYQMLYSGGSFRLPQSSGH 586
            TFFILSGVAGLGSIVSMILTVRLRPVYQMLYSGGSFRLPQSSGH
Sbjct: 541 MTFFILSGVAGLGSIVSMILTVRLRPVYQMLYSGGSFRLPQSSGH 565

BLAST of CaUC07G136690 vs. NCBI nr
Match: XP_004152187.1 (protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis sativus] >KGN52923.1 hypothetical protein Csa_014743 [Cucumis sativus])

HSP 1 Score: 1015.4 bits (2624), Expect = 2.0e-292
Identity = 530/585 (90.60%), Postives = 555/585 (94.87%), Query Frame = 0

Query: 1   MPKLVLKGGSRPPWVGLAAAVWVQIAGGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI 60
           MPKLVLKGG+RPPWVGLAAAVWVQIAGGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI
Sbjct: 1   MPKLVLKGGTRPPWVGLAAAVWVQIAGGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI 60

Query: 61  GESMGLVPGLACNRFPPWVILLFGAFCCFLGYGAIWLAVSRTVPNLPYWLVSLMFIFSLC 120
           GESMGL+PGLACN+FPPWVILLFGAFCCF+GYGAIWLAVSRTVPNLPYW           
Sbjct: 61  GESMGLIPGLACNKFPPWVILLFGAFCCFIGYGAIWLAVSRTVPNLPYW----------- 120

Query: 121 EDLSLIFLSVLLAADFHELLSNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYT 180
               L++L+  +A +     SNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYT
Sbjct: 121 ----LLWLTHCVATN-----SNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYT 180

Query: 181 VIYSMVLKKSALNLLLFLAIGIPILCLALMYFVRPCTPASSEDPSERAHFLFTQAACVLL 240
           VIYS+VL+KSALNLLLFLAIGIPILCLA+MYFVRPCTPASSEDPSERAHFLFTQAACVLL
Sbjct: 181 VIYSLVLRKSALNLLLFLAIGIPILCLAMMYFVRPCTPASSEDPSERAHFLFTQAACVLL 240

Query: 241 AVFLVSTTILDATTTPSDAVGYTLVAIMVILLMSPLAVPIKMTICAKSKKLGPRVDSAEP 300
            +FLVSTTILDATTTPSDAVGYTLVAIMVILLMSPLAVPIKMTICA++K LGPRVDS+EP
Sbjct: 241 GLFLVSTTILDATTTPSDAVGYTLVAIMVILLMSPLAVPIKMTICARTKTLGPRVDSSEP 300

Query: 301 LASGESDSSQIEPLLTPSSSATNLGSFCENDDSSDVETLLAVGEGAIHKKKRRPKRGEDF 360
           LASGESDSSQIEPLLTPSSSATNLGSF ENDD+SDVETLLAVGEGAIHKKKRRPKRGEDF
Sbjct: 301 LASGESDSSQIEPLLTPSSSATNLGSFYENDDASDVETLLAVGEGAIHKKKRRPKRGEDF 360

Query: 361 KLREAVIKADFWLLWFLYFLGVGPGITILNNLSQIGISLGVNDTTLLLALFSFCNFVGRL 420
           KLREAVIKADFWLLWFLYFLGVGPGIT+LNNLSQIGISLG+ND TLLLALFSFCNFVGRL
Sbjct: 361 KLREAVIKADFWLLWFLYFLGVGPGITVLNNLSQIGISLGINDATLLLALFSFCNFVGRL 420

Query: 421 GSGVISEHFVRSRMIPRSLWMMFALILMAIAFLLYASALTGTLYVATGLTGISYGVLYSM 480
           GSGVISEHFVRSRMIPRSLWMMFAL+LM+IAFLLYASALT TLY+ATGLTGISYGVLYSM
Sbjct: 421 GSGVISEHFVRSRMIPRSLWMMFALVLMSIAFLLYASALTITLYIATGLTGISYGVLYSM 480

Query: 481 MVPLASEIFGLKNFGVIFNFMQLGNPIGAVLFSVLLASTLYDNEAAKQGSITCIGQQCFR 540
           MVPLASEIFGLKNFGVIFNFMQLGNPIGAVLFSVLL STLYD EAAKQGSITCIGQQCFR
Sbjct: 481 MVPLASEIFGLKNFGVIFNFMQLGNPIGAVLFSVLLTSTLYDIEAAKQGSITCIGQQCFR 540

Query: 541 TTFFILSGVAGLGSIVSMILTVRLRPVYQMLYSGGSFRLPQSSGH 586
           TTFFILSGVAGLGSIVS+ILT+RLRPVYQMLY+GGSFRLPQSSGH
Sbjct: 541 TTFFILSGVAGLGSIVSLILTIRLRPVYQMLYAGGSFRLPQSSGH 565

BLAST of CaUC07G136690 vs. NCBI nr
Match: TYK29592.1 (protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis melo var. makuwa])

HSP 1 Score: 1013.1 bits (2618), Expect = 9.9e-292
Identity = 530/585 (90.60%), Postives = 552/585 (94.36%), Query Frame = 0

Query: 1   MPKLVLKGGSRPPWVGLAAAVWVQIAGGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI 60
           MPKLVLKGG+RPPWVGLAAAVWVQIAGGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI
Sbjct: 1   MPKLVLKGGTRPPWVGLAAAVWVQIAGGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI 60

Query: 61  GESMGLVPGLACNRFPPWVILLFGAFCCFLGYGAIWLAVSRTVPNLPYWLVSLMFIFSLC 120
           GESMGLVPGLACNRFPPWVILLFGAFCCF+GYGAIWLAVSRTVPNLPYW           
Sbjct: 61  GESMGLVPGLACNRFPPWVILLFGAFCCFIGYGAIWLAVSRTVPNLPYW----------- 120

Query: 121 EDLSLIFLSVLLAADFHELLSNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYT 180
               L++L+  +A +     SNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYT
Sbjct: 121 ----LLWLTHCIATN-----SNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYT 180

Query: 181 VIYSMVLKKSALNLLLFLAIGIPILCLALMYFVRPCTPASSEDPSERAHFLFTQAACVLL 240
           VIYS+VLKKSALNLLLFL IGIPILCLALMYFVRPCTPAS+EDPSERAHFLFTQAACVLL
Sbjct: 181 VIYSLVLKKSALNLLLFLTIGIPILCLALMYFVRPCTPASTEDPSERAHFLFTQAACVLL 240

Query: 241 AVFLVSTTILDATTTPSDAVGYTLVAIMVILLMSPLAVPIKMTICAKSKKLGPRVDSAEP 300
            +FLVSTTILDATTTPSDAVGYTLVAIMVILLMSPLAVPIKMTICAK+K +GPRVDS+EP
Sbjct: 241 GLFLVSTTILDATTTPSDAVGYTLVAIMVILLMSPLAVPIKMTICAKTKTVGPRVDSSEP 300

Query: 301 LASGESDSSQIEPLLTPSSSATNLGSFCENDDSSDVETLLAVGEGAIHKKKRRPKRGEDF 360
           LASG SDSSQIEPLLTPSSSATNLGSF ENDD+SDVETLLAVGEGAIHKKKRRPKRGEDF
Sbjct: 301 LASGGSDSSQIEPLLTPSSSATNLGSFYENDDASDVETLLAVGEGAIHKKKRRPKRGEDF 360

Query: 361 KLREAVIKADFWLLWFLYFLGVGPGITILNNLSQIGISLGVNDTTLLLALFSFCNFVGRL 420
           KLREAVIKADFWLLWFLYFLGVGPGIT+LNNLSQIGISLG+NDTTLLLALFSFCNFVGRL
Sbjct: 361 KLREAVIKADFWLLWFLYFLGVGPGITVLNNLSQIGISLGINDTTLLLALFSFCNFVGRL 420

Query: 421 GSGVISEHFVRSRMIPRSLWMMFALILMAIAFLLYASALTGTLYVATGLTGISYGVLYSM 480
           GSGVISEHFVRSRMIPRSLWMMFAL+LM+IAFLLYAS LT TLY+ATGLTGISYGVLYSM
Sbjct: 421 GSGVISEHFVRSRMIPRSLWMMFALVLMSIAFLLYASGLTITLYIATGLTGISYGVLYSM 480

Query: 481 MVPLASEIFGLKNFGVIFNFMQLGNPIGAVLFSVLLASTLYDNEAAKQGSITCIGQQCFR 540
           MVPLASEIFGLKNFGVIFNFMQLGNPIGAVLFSVLL STLYD EAA QGSITCIGQQCFR
Sbjct: 481 MVPLASEIFGLKNFGVIFNFMQLGNPIGAVLFSVLLTSTLYDKEAAMQGSITCIGQQCFR 540

Query: 541 TTFFILSGVAGLGSIVSMILTVRLRPVYQMLYSGGSFRLPQSSGH 586
           TTFFILSGVAGLGSIVS+ILT+RLRPVYQMLY+GGSFRLPQSSGH
Sbjct: 541 TTFFILSGVAGLGSIVSLILTIRLRPVYQMLYAGGSFRLPQSSGH 565

BLAST of CaUC07G136690 vs. NCBI nr
Match: XP_008454196.2 (PREDICTED: LOW QUALITY PROTEIN: protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis melo])

HSP 1 Score: 1011.5 bits (2614), Expect = 2.9e-291
Identity = 530/585 (90.60%), Postives = 551/585 (94.19%), Query Frame = 0

Query: 1   MPKLVLKGGSRPPWVGLAAAVWVQIAGGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI 60
           MPKLVLKGG+RPPWVGLAAAVWVQIAGGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI
Sbjct: 1   MPKLVLKGGTRPPWVGLAAAVWVQIAGGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI 60

Query: 61  GESMGLVPGLACNRFPPWVILLFGAFCCFLGYGAIWLAVSRTVPNLPYWLVSLMFIFSLC 120
           GESMGLVPGLACNRFPPWVILLFGAFCCF+GYGAIWLAVSRTVPNLPYW           
Sbjct: 61  GESMGLVPGLACNRFPPWVILLFGAFCCFIGYGAIWLAVSRTVPNLPYW----------- 120

Query: 121 EDLSLIFLSVLLAADFHELLSNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYT 180
               L++L+  +A +     SNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYT
Sbjct: 121 ----LLWLTHCIATN-----SNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYT 180

Query: 181 VIYSMVLKKSALNLLLFLAIGIPILCLALMYFVRPCTPASSEDPSERAHFLFTQAACVLL 240
           VIYS+VLKKSALNLLLFL IGIPILCLALMYFVRPCTPAS+EDPSERAHFLFTQAACVLL
Sbjct: 181 VIYSLVLKKSALNLLLFLTIGIPILCLALMYFVRPCTPASTEDPSERAHFLFTQAACVLL 240

Query: 241 AVFLVSTTILDATTTPSDAVGYTLVAIMVILLMSPLAVPIKMTICAKSKKLGPRVDSAEP 300
             FLVSTTILDATTTPSDAVGYTLVAIMVILLMSPLAVPIKMTICAK+K +GPRVDS+EP
Sbjct: 241 XSFLVSTTILDATTTPSDAVGYTLVAIMVILLMSPLAVPIKMTICAKTKTVGPRVDSSEP 300

Query: 301 LASGESDSSQIEPLLTPSSSATNLGSFCENDDSSDVETLLAVGEGAIHKKKRRPKRGEDF 360
           LASG SDSSQIEPLLTPSSSATNLGSF ENDD+SDVETLLAVGEGAIHKKKRRPKRGEDF
Sbjct: 301 LASGGSDSSQIEPLLTPSSSATNLGSFYENDDASDVETLLAVGEGAIHKKKRRPKRGEDF 360

Query: 361 KLREAVIKADFWLLWFLYFLGVGPGITILNNLSQIGISLGVNDTTLLLALFSFCNFVGRL 420
           KLREAVIKADFWLLWFLYFLGVGPGIT+LNNLSQIGISLG+NDTTLLLALFSFCNFVGRL
Sbjct: 361 KLREAVIKADFWLLWFLYFLGVGPGITVLNNLSQIGISLGINDTTLLLALFSFCNFVGRL 420

Query: 421 GSGVISEHFVRSRMIPRSLWMMFALILMAIAFLLYASALTGTLYVATGLTGISYGVLYSM 480
           GSGVISEHFVRSRMIPRSLWMMFAL+LM+IAFLLYAS LT TLY+ATGLTGISYGVLYSM
Sbjct: 421 GSGVISEHFVRSRMIPRSLWMMFALVLMSIAFLLYASGLTITLYIATGLTGISYGVLYSM 480

Query: 481 MVPLASEIFGLKNFGVIFNFMQLGNPIGAVLFSVLLASTLYDNEAAKQGSITCIGQQCFR 540
           MVPLASEIFGLKNFGVIFNFMQLGNPIGAVLFSVLL STLYD EAA QGSITCIGQQCFR
Sbjct: 481 MVPLASEIFGLKNFGVIFNFMQLGNPIGAVLFSVLLTSTLYDKEAAMQGSITCIGQQCFR 540

Query: 541 TTFFILSGVAGLGSIVSMILTVRLRPVYQMLYSGGSFRLPQSSGH 586
           TTFFILSGVAGLGSIVS+ILT+RLRPVYQMLY+GGSFRLPQSSGH
Sbjct: 541 TTFFILSGVAGLGSIVSLILTIRLRPVYQMLYAGGSFRLPQSSGH 565

BLAST of CaUC07G136690 vs. NCBI nr
Match: KAA0044465.1 (protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis melo var. makuwa])

HSP 1 Score: 1008.4 bits (2606), Expect = 2.4e-290
Identity = 528/585 (90.26%), Postives = 550/585 (94.02%), Query Frame = 0

Query: 1   MPKLVLKGGSRPPWVGLAAAVWVQIAGGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI 60
           MPKLVLKGG+RPPWVGLAAAVWVQIAGGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI
Sbjct: 1   MPKLVLKGGTRPPWVGLAAAVWVQIAGGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI 60

Query: 61  GESMGLVPGLACNRFPPWVILLFGAFCCFLGYGAIWLAVSRTVPNLPYWLVSLMFIFSLC 120
           GESMGLVPGLACNRFPPWVILLFGAFCCF+GYGAIWLAVSRTVPNLPYW           
Sbjct: 61  GESMGLVPGLACNRFPPWVILLFGAFCCFIGYGAIWLAVSRTVPNLPYW----------- 120

Query: 121 EDLSLIFLSVLLAADFHELLSNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYT 180
               L++L+  +A +     SNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYT
Sbjct: 121 ----LLWLTHCIATN-----SNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYT 180

Query: 181 VIYSMVLKKSALNLLLFLAIGIPILCLALMYFVRPCTPASSEDPSERAHFLFTQAACVLL 240
           VIYS+VLKKSALNLLLFL IGIPILCLALMYFVRPCTPAS+EDPSERAHFLFTQAACVLL
Sbjct: 181 VIYSLVLKKSALNLLLFLTIGIPILCLALMYFVRPCTPASTEDPSERAHFLFTQAACVLL 240

Query: 241 AVFLVSTTILDATTTPSDAVGYTLVAIMVILLMSPLAVPIKMTICAKSKKLGPRVDSAEP 300
            +FLVSTTILD TTTPSDAVGYTLVAIMVILLMSPLAVPIKMTICAK+K +GPRVDS+EP
Sbjct: 241 GLFLVSTTILDGTTTPSDAVGYTLVAIMVILLMSPLAVPIKMTICAKTKTVGPRVDSSEP 300

Query: 301 LASGESDSSQIEPLLTPSSSATNLGSFCENDDSSDVETLLAVGEGAIHKKKRRPKRGEDF 360
           LASG SDSSQIEPLLTPSSSATNLGSF ENDD+SDVETLLAVGEGAIHKKKRRPKRGEDF
Sbjct: 301 LASGGSDSSQIEPLLTPSSSATNLGSFYENDDASDVETLLAVGEGAIHKKKRRPKRGEDF 360

Query: 361 KLREAVIKADFWLLWFLYFLGVGPGITILNNLSQIGISLGVNDTTLLLALFSFCNFVGRL 420
           KL EAVIKADFWLLWFLYFLGVGPGIT+LNNLSQIGISLG+NDTTLLLALFSFCNFVGRL
Sbjct: 361 KLCEAVIKADFWLLWFLYFLGVGPGITVLNNLSQIGISLGINDTTLLLALFSFCNFVGRL 420

Query: 421 GSGVISEHFVRSRMIPRSLWMMFALILMAIAFLLYASALTGTLYVATGLTGISYGVLYSM 480
           GSGVISEHFVRSRMIPRSLWMMFAL+LM+IAFLLYAS LT TLY+ATGLTGISYGVLYSM
Sbjct: 421 GSGVISEHFVRSRMIPRSLWMMFALVLMSIAFLLYASGLTITLYIATGLTGISYGVLYSM 480

Query: 481 MVPLASEIFGLKNFGVIFNFMQLGNPIGAVLFSVLLASTLYDNEAAKQGSITCIGQQCFR 540
           MVPLASEIFGLKNFGVIFNFMQLGNPIGAVLFSVLL STLYD EAA QGSITCIGQQCFR
Sbjct: 481 MVPLASEIFGLKNFGVIFNFMQLGNPIGAVLFSVLLTSTLYDKEAAMQGSITCIGQQCFR 540

Query: 541 TTFFILSGVAGLGSIVSMILTVRLRPVYQMLYSGGSFRLPQSSGH 586
           TTFFILSGVAGLGSIVS+ILT+RLRPVYQMLY+GGSFRLPQSSGH
Sbjct: 541 TTFFILSGVAGLGSIVSLILTIRLRPVYQMLYAGGSFRLPQSSGH 565

BLAST of CaUC07G136690 vs. ExPASy Swiss-Prot
Match: F4I9E1 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 196.1 bits (497), Expect = 1.1e-48
Identity = 178/588 (30.27%), Postives = 285/588 (48.47%), Query Frame = 0

Query: 1   MPKLVLKGGSRPPWVGLAAAVWVQIAGGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI 60
           +P L    G    W  L AA+W+Q + G++++FS YS  LKSVLG++Q +L  L VA+D+
Sbjct: 31  LPPLRTMFGRWRKWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDL 90

Query: 61  GESMGLVPGLACNRFPPWVILLFGAFCCFLGYGAIWLAVSRTVPNLPYWLVSLMFIFSLC 120
           G++ G   G+A   FP  V+L   A   F+GYG  WL ++  +  LPY            
Sbjct: 91  GKAFGWSSGIALGYFPLSVVLFAAAAMGFVGYGVQWLVITNII-TLPY------------ 150

Query: 121 EDLSLIFLSVLLAADFHELLSNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYT 180
              SL+FL  LLA      LS  WF TA  +  +R+FP +R     +   + G+SAA+Y+
Sbjct: 151 ---SLVFLCCLLAG-----LSICWFNTACFILCIRHFPNNRALALSLTVSFNGISAALYS 210

Query: 181 VIYSMVLKKSALNLLLFLAIGIP-ILCLALMY--FVRPCTPASSEDPSERAHFLFTQAAC 240
           + ++  +  S+ NL L L   +P ++  A +Y    +P    ++ D   R H        
Sbjct: 211 LAFN-AINPSSSNLYLLLNSLVPLVVSFAALYPVLTKPSLD-TTPDYDSRRHDSHVFTIL 270

Query: 241 VLLAVFLVSTTILDATTTPSDAVGYTLVAIMVILLMSPLAVPIKMTICAKSKKLGPRVDS 300
            +LAV      +L +++T S  + +      V+LL+ PL  P  + + A+   L      
Sbjct: 271 NVLAVITSFHLLLSSSSTSSARLNFIGA---VVLLVFPLCAP--LLVYARDYFL------ 330

Query: 301 AEPLASGESDSSQIEPLLTPSSSATNLGSFCEN-DDSSDVETLLAVGEGAIH----KKKR 360
                          P++    +  + G    N D+  + +T ++   G  H    K+  
Sbjct: 331 ---------------PVINARLNHESSGYVMLNIDELKNQKTSVSSKTGYEHMGTAKEGN 390

Query: 361 RPKRGEDFKLREAVIKADFWLLWFLYFLGVGPGITILNNLSQIGISLGVNDTTLLLALFS 420
             + G++   R  + + +FWL +  YF G   G+   NNL QI  SLG N TT L+ ++S
Sbjct: 391 TVRLGDEHSFRLLISRLEFWLYYIAYFCGGTIGLVYSNNLGQIAQSLGQNSTT-LVTIYS 450

Query: 421 FCNFVGRLGSGVISEHFVRSRM-IPRSLWMMFALILMAIAFLLYA--SALTGTLYVATGL 480
             +F GRL S   +  F+  R  + R+ W   AL+   IAF L A  S+    L  AT L
Sbjct: 451 SFSFFGRLLSA--APDFMHKRFRLTRTGWFAIALLPTPIAFFLLAVSSSQQTALQTATAL 510

Query: 481 TGISYGVLYSMMVPLASEIFGLKNFGVIFNFMQLGNPIGAVLFSVLLASTLYDNEAAK-- 540
            G+S G +++  V + S++FG  + GV  N +    PIG++L+  + AS +Y+  A+   
Sbjct: 511 IGLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIPIGSLLYGYIAAS-IYEANASPDI 565

Query: 541 ----QGSITCIGQQCFRTTFFILSGVAGLGSIVSMILTVRLRPVYQML 572
                 SI CIG+ C+  TF     ++ LG + S+ L +R +PVY  L
Sbjct: 571 TPIVSDSIVCIGRDCYFKTFVFWGCLSILGVVSSLSLYIRTKPVYHRL 565

BLAST of CaUC07G136690 vs. ExPASy Swiss-Prot
Match: Q5AXV1 (Probable transporter mch1 OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=mch1 PE=3 SV=2)

HSP 1 Score: 52.4 bits (124), Expect = 2.0e-05
Identity = 58/262 (22.14%), Postives = 116/262 (44.27%), Query Frame = 0

Query: 329 ENDDSSDVETLLAVGEGAIHKKKRRPKRGEDFKLRE---------AVIKADFWLLWFL-- 388
           +++D+  V+         + +  RR K  E+ + R+          +  AD  + W    
Sbjct: 325 DHEDNGSVDDQSVTISEELREAARREKEREEEERRKKNWLLNYETRIFLADHTMWWLALG 384

Query: 389 YFLGVGPGITILNNLSQIGISLGVNDTTLL-----------LALFSFCNFVGRLGSGVIS 448
           +FL  GPG   LNNL  I  +L ++ T ++           + + +  + + RL +G +S
Sbjct: 385 FFLVTGPGEAYLNNLGTIVQTLNLDTTAIVDSHPAGLPSTHVTIIALTSTIARLLTGSLS 444

Query: 449 E--------HFV-------------RSRMIPRSLWMMFALILMAIAFLLYASAL----TG 508
           +        HF              +   + R  +++ + +L+++ FLL AS L      
Sbjct: 445 DLFAPTARRHFTVDQETAGPDPFTKQRPALSRLAFLIPSALLLSLGFLLLASPLPTHHPE 504

Query: 509 TLYVATGLTGISYGVLYSMMVPLASEIFGLKNFGVIFNFMQLGNPIGAVLFSVLLASTLY 540
             ++ T L G+ YG ++S++  + S ++G++NFG  +  + +    GA ++ V+ +    
Sbjct: 505 LSHLTTALVGLGYGSIFSLVPIIISVVWGVENFGTNWGIVAMFPAAGAAMWGVIYSRAY- 564

BLAST of CaUC07G136690 vs. ExPASy TrEMBL
Match: A0A0A0KTP3 (Nodulin-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G006300 PE=4 SV=1)

HSP 1 Score: 1015.4 bits (2624), Expect = 9.7e-293
Identity = 530/585 (90.60%), Postives = 555/585 (94.87%), Query Frame = 0

Query: 1   MPKLVLKGGSRPPWVGLAAAVWVQIAGGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI 60
           MPKLVLKGG+RPPWVGLAAAVWVQIAGGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI
Sbjct: 1   MPKLVLKGGTRPPWVGLAAAVWVQIAGGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI 60

Query: 61  GESMGLVPGLACNRFPPWVILLFGAFCCFLGYGAIWLAVSRTVPNLPYWLVSLMFIFSLC 120
           GESMGL+PGLACN+FPPWVILLFGAFCCF+GYGAIWLAVSRTVPNLPYW           
Sbjct: 61  GESMGLIPGLACNKFPPWVILLFGAFCCFIGYGAIWLAVSRTVPNLPYW----------- 120

Query: 121 EDLSLIFLSVLLAADFHELLSNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYT 180
               L++L+  +A +     SNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYT
Sbjct: 121 ----LLWLTHCVATN-----SNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYT 180

Query: 181 VIYSMVLKKSALNLLLFLAIGIPILCLALMYFVRPCTPASSEDPSERAHFLFTQAACVLL 240
           VIYS+VL+KSALNLLLFLAIGIPILCLA+MYFVRPCTPASSEDPSERAHFLFTQAACVLL
Sbjct: 181 VIYSLVLRKSALNLLLFLAIGIPILCLAMMYFVRPCTPASSEDPSERAHFLFTQAACVLL 240

Query: 241 AVFLVSTTILDATTTPSDAVGYTLVAIMVILLMSPLAVPIKMTICAKSKKLGPRVDSAEP 300
            +FLVSTTILDATTTPSDAVGYTLVAIMVILLMSPLAVPIKMTICA++K LGPRVDS+EP
Sbjct: 241 GLFLVSTTILDATTTPSDAVGYTLVAIMVILLMSPLAVPIKMTICARTKTLGPRVDSSEP 300

Query: 301 LASGESDSSQIEPLLTPSSSATNLGSFCENDDSSDVETLLAVGEGAIHKKKRRPKRGEDF 360
           LASGESDSSQIEPLLTPSSSATNLGSF ENDD+SDVETLLAVGEGAIHKKKRRPKRGEDF
Sbjct: 301 LASGESDSSQIEPLLTPSSSATNLGSFYENDDASDVETLLAVGEGAIHKKKRRPKRGEDF 360

Query: 361 KLREAVIKADFWLLWFLYFLGVGPGITILNNLSQIGISLGVNDTTLLLALFSFCNFVGRL 420
           KLREAVIKADFWLLWFLYFLGVGPGIT+LNNLSQIGISLG+ND TLLLALFSFCNFVGRL
Sbjct: 361 KLREAVIKADFWLLWFLYFLGVGPGITVLNNLSQIGISLGINDATLLLALFSFCNFVGRL 420

Query: 421 GSGVISEHFVRSRMIPRSLWMMFALILMAIAFLLYASALTGTLYVATGLTGISYGVLYSM 480
           GSGVISEHFVRSRMIPRSLWMMFAL+LM+IAFLLYASALT TLY+ATGLTGISYGVLYSM
Sbjct: 421 GSGVISEHFVRSRMIPRSLWMMFALVLMSIAFLLYASALTITLYIATGLTGISYGVLYSM 480

Query: 481 MVPLASEIFGLKNFGVIFNFMQLGNPIGAVLFSVLLASTLYDNEAAKQGSITCIGQQCFR 540
           MVPLASEIFGLKNFGVIFNFMQLGNPIGAVLFSVLL STLYD EAAKQGSITCIGQQCFR
Sbjct: 481 MVPLASEIFGLKNFGVIFNFMQLGNPIGAVLFSVLLTSTLYDIEAAKQGSITCIGQQCFR 540

Query: 541 TTFFILSGVAGLGSIVSMILTVRLRPVYQMLYSGGSFRLPQSSGH 586
           TTFFILSGVAGLGSIVS+ILT+RLRPVYQMLY+GGSFRLPQSSGH
Sbjct: 541 TTFFILSGVAGLGSIVSLILTIRLRPVYQMLYAGGSFRLPQSSGH 565

BLAST of CaUC07G136690 vs. ExPASy TrEMBL
Match: A0A5D3E0Y7 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G002010 PE=4 SV=1)

HSP 1 Score: 1013.1 bits (2618), Expect = 4.8e-292
Identity = 530/585 (90.60%), Postives = 552/585 (94.36%), Query Frame = 0

Query: 1   MPKLVLKGGSRPPWVGLAAAVWVQIAGGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI 60
           MPKLVLKGG+RPPWVGLAAAVWVQIAGGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI
Sbjct: 1   MPKLVLKGGTRPPWVGLAAAVWVQIAGGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI 60

Query: 61  GESMGLVPGLACNRFPPWVILLFGAFCCFLGYGAIWLAVSRTVPNLPYWLVSLMFIFSLC 120
           GESMGLVPGLACNRFPPWVILLFGAFCCF+GYGAIWLAVSRTVPNLPYW           
Sbjct: 61  GESMGLVPGLACNRFPPWVILLFGAFCCFIGYGAIWLAVSRTVPNLPYW----------- 120

Query: 121 EDLSLIFLSVLLAADFHELLSNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYT 180
               L++L+  +A +     SNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYT
Sbjct: 121 ----LLWLTHCIATN-----SNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYT 180

Query: 181 VIYSMVLKKSALNLLLFLAIGIPILCLALMYFVRPCTPASSEDPSERAHFLFTQAACVLL 240
           VIYS+VLKKSALNLLLFL IGIPILCLALMYFVRPCTPAS+EDPSERAHFLFTQAACVLL
Sbjct: 181 VIYSLVLKKSALNLLLFLTIGIPILCLALMYFVRPCTPASTEDPSERAHFLFTQAACVLL 240

Query: 241 AVFLVSTTILDATTTPSDAVGYTLVAIMVILLMSPLAVPIKMTICAKSKKLGPRVDSAEP 300
            +FLVSTTILDATTTPSDAVGYTLVAIMVILLMSPLAVPIKMTICAK+K +GPRVDS+EP
Sbjct: 241 GLFLVSTTILDATTTPSDAVGYTLVAIMVILLMSPLAVPIKMTICAKTKTVGPRVDSSEP 300

Query: 301 LASGESDSSQIEPLLTPSSSATNLGSFCENDDSSDVETLLAVGEGAIHKKKRRPKRGEDF 360
           LASG SDSSQIEPLLTPSSSATNLGSF ENDD+SDVETLLAVGEGAIHKKKRRPKRGEDF
Sbjct: 301 LASGGSDSSQIEPLLTPSSSATNLGSFYENDDASDVETLLAVGEGAIHKKKRRPKRGEDF 360

Query: 361 KLREAVIKADFWLLWFLYFLGVGPGITILNNLSQIGISLGVNDTTLLLALFSFCNFVGRL 420
           KLREAVIKADFWLLWFLYFLGVGPGIT+LNNLSQIGISLG+NDTTLLLALFSFCNFVGRL
Sbjct: 361 KLREAVIKADFWLLWFLYFLGVGPGITVLNNLSQIGISLGINDTTLLLALFSFCNFVGRL 420

Query: 421 GSGVISEHFVRSRMIPRSLWMMFALILMAIAFLLYASALTGTLYVATGLTGISYGVLYSM 480
           GSGVISEHFVRSRMIPRSLWMMFAL+LM+IAFLLYAS LT TLY+ATGLTGISYGVLYSM
Sbjct: 421 GSGVISEHFVRSRMIPRSLWMMFALVLMSIAFLLYASGLTITLYIATGLTGISYGVLYSM 480

Query: 481 MVPLASEIFGLKNFGVIFNFMQLGNPIGAVLFSVLLASTLYDNEAAKQGSITCIGQQCFR 540
           MVPLASEIFGLKNFGVIFNFMQLGNPIGAVLFSVLL STLYD EAA QGSITCIGQQCFR
Sbjct: 481 MVPLASEIFGLKNFGVIFNFMQLGNPIGAVLFSVLLTSTLYDKEAAMQGSITCIGQQCFR 540

Query: 541 TTFFILSGVAGLGSIVSMILTVRLRPVYQMLYSGGSFRLPQSSGH 586
           TTFFILSGVAGLGSIVS+ILT+RLRPVYQMLY+GGSFRLPQSSGH
Sbjct: 541 TTFFILSGVAGLGSIVSLILTIRLRPVYQMLYAGGSFRLPQSSGH 565

BLAST of CaUC07G136690 vs. ExPASy TrEMBL
Match: A0A1S3BY60 (LOW QUALITY PROTEIN: protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo OX=3656 GN=LOC103494674 PE=4 SV=1)

HSP 1 Score: 1011.5 bits (2614), Expect = 1.4e-291
Identity = 530/585 (90.60%), Postives = 551/585 (94.19%), Query Frame = 0

Query: 1   MPKLVLKGGSRPPWVGLAAAVWVQIAGGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI 60
           MPKLVLKGG+RPPWVGLAAAVWVQIAGGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI
Sbjct: 1   MPKLVLKGGTRPPWVGLAAAVWVQIAGGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI 60

Query: 61  GESMGLVPGLACNRFPPWVILLFGAFCCFLGYGAIWLAVSRTVPNLPYWLVSLMFIFSLC 120
           GESMGLVPGLACNRFPPWVILLFGAFCCF+GYGAIWLAVSRTVPNLPYW           
Sbjct: 61  GESMGLVPGLACNRFPPWVILLFGAFCCFIGYGAIWLAVSRTVPNLPYW----------- 120

Query: 121 EDLSLIFLSVLLAADFHELLSNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYT 180
               L++L+  +A +     SNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYT
Sbjct: 121 ----LLWLTHCIATN-----SNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYT 180

Query: 181 VIYSMVLKKSALNLLLFLAIGIPILCLALMYFVRPCTPASSEDPSERAHFLFTQAACVLL 240
           VIYS+VLKKSALNLLLFL IGIPILCLALMYFVRPCTPAS+EDPSERAHFLFTQAACVLL
Sbjct: 181 VIYSLVLKKSALNLLLFLTIGIPILCLALMYFVRPCTPASTEDPSERAHFLFTQAACVLL 240

Query: 241 AVFLVSTTILDATTTPSDAVGYTLVAIMVILLMSPLAVPIKMTICAKSKKLGPRVDSAEP 300
             FLVSTTILDATTTPSDAVGYTLVAIMVILLMSPLAVPIKMTICAK+K +GPRVDS+EP
Sbjct: 241 XSFLVSTTILDATTTPSDAVGYTLVAIMVILLMSPLAVPIKMTICAKTKTVGPRVDSSEP 300

Query: 301 LASGESDSSQIEPLLTPSSSATNLGSFCENDDSSDVETLLAVGEGAIHKKKRRPKRGEDF 360
           LASG SDSSQIEPLLTPSSSATNLGSF ENDD+SDVETLLAVGEGAIHKKKRRPKRGEDF
Sbjct: 301 LASGGSDSSQIEPLLTPSSSATNLGSFYENDDASDVETLLAVGEGAIHKKKRRPKRGEDF 360

Query: 361 KLREAVIKADFWLLWFLYFLGVGPGITILNNLSQIGISLGVNDTTLLLALFSFCNFVGRL 420
           KLREAVIKADFWLLWFLYFLGVGPGIT+LNNLSQIGISLG+NDTTLLLALFSFCNFVGRL
Sbjct: 361 KLREAVIKADFWLLWFLYFLGVGPGITVLNNLSQIGISLGINDTTLLLALFSFCNFVGRL 420

Query: 421 GSGVISEHFVRSRMIPRSLWMMFALILMAIAFLLYASALTGTLYVATGLTGISYGVLYSM 480
           GSGVISEHFVRSRMIPRSLWMMFAL+LM+IAFLLYAS LT TLY+ATGLTGISYGVLYSM
Sbjct: 421 GSGVISEHFVRSRMIPRSLWMMFALVLMSIAFLLYASGLTITLYIATGLTGISYGVLYSM 480

Query: 481 MVPLASEIFGLKNFGVIFNFMQLGNPIGAVLFSVLLASTLYDNEAAKQGSITCIGQQCFR 540
           MVPLASEIFGLKNFGVIFNFMQLGNPIGAVLFSVLL STLYD EAA QGSITCIGQQCFR
Sbjct: 481 MVPLASEIFGLKNFGVIFNFMQLGNPIGAVLFSVLLTSTLYDKEAAMQGSITCIGQQCFR 540

Query: 541 TTFFILSGVAGLGSIVSMILTVRLRPVYQMLYSGGSFRLPQSSGH 586
           TTFFILSGVAGLGSIVS+ILT+RLRPVYQMLY+GGSFRLPQSSGH
Sbjct: 541 TTFFILSGVAGLGSIVSLILTIRLRPVYQMLYAGGSFRLPQSSGH 565

BLAST of CaUC07G136690 vs. ExPASy TrEMBL
Match: A0A5A7TSX4 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G002010 PE=4 SV=1)

HSP 1 Score: 1008.4 bits (2606), Expect = 1.2e-290
Identity = 528/585 (90.26%), Postives = 550/585 (94.02%), Query Frame = 0

Query: 1   MPKLVLKGGSRPPWVGLAAAVWVQIAGGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI 60
           MPKLVLKGG+RPPWVGLAAAVWVQIAGGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI
Sbjct: 1   MPKLVLKGGTRPPWVGLAAAVWVQIAGGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI 60

Query: 61  GESMGLVPGLACNRFPPWVILLFGAFCCFLGYGAIWLAVSRTVPNLPYWLVSLMFIFSLC 120
           GESMGLVPGLACNRFPPWVILLFGAFCCF+GYGAIWLAVSRTVPNLPYW           
Sbjct: 61  GESMGLVPGLACNRFPPWVILLFGAFCCFIGYGAIWLAVSRTVPNLPYW----------- 120

Query: 121 EDLSLIFLSVLLAADFHELLSNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYT 180
               L++L+  +A +     SNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYT
Sbjct: 121 ----LLWLTHCIATN-----SNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYT 180

Query: 181 VIYSMVLKKSALNLLLFLAIGIPILCLALMYFVRPCTPASSEDPSERAHFLFTQAACVLL 240
           VIYS+VLKKSALNLLLFL IGIPILCLALMYFVRPCTPAS+EDPSERAHFLFTQAACVLL
Sbjct: 181 VIYSLVLKKSALNLLLFLTIGIPILCLALMYFVRPCTPASTEDPSERAHFLFTQAACVLL 240

Query: 241 AVFLVSTTILDATTTPSDAVGYTLVAIMVILLMSPLAVPIKMTICAKSKKLGPRVDSAEP 300
            +FLVSTTILD TTTPSDAVGYTLVAIMVILLMSPLAVPIKMTICAK+K +GPRVDS+EP
Sbjct: 241 GLFLVSTTILDGTTTPSDAVGYTLVAIMVILLMSPLAVPIKMTICAKTKTVGPRVDSSEP 300

Query: 301 LASGESDSSQIEPLLTPSSSATNLGSFCENDDSSDVETLLAVGEGAIHKKKRRPKRGEDF 360
           LASG SDSSQIEPLLTPSSSATNLGSF ENDD+SDVETLLAVGEGAIHKKKRRPKRGEDF
Sbjct: 301 LASGGSDSSQIEPLLTPSSSATNLGSFYENDDASDVETLLAVGEGAIHKKKRRPKRGEDF 360

Query: 361 KLREAVIKADFWLLWFLYFLGVGPGITILNNLSQIGISLGVNDTTLLLALFSFCNFVGRL 420
           KL EAVIKADFWLLWFLYFLGVGPGIT+LNNLSQIGISLG+NDTTLLLALFSFCNFVGRL
Sbjct: 361 KLCEAVIKADFWLLWFLYFLGVGPGITVLNNLSQIGISLGINDTTLLLALFSFCNFVGRL 420

Query: 421 GSGVISEHFVRSRMIPRSLWMMFALILMAIAFLLYASALTGTLYVATGLTGISYGVLYSM 480
           GSGVISEHFVRSRMIPRSLWMMFAL+LM+IAFLLYAS LT TLY+ATGLTGISYGVLYSM
Sbjct: 421 GSGVISEHFVRSRMIPRSLWMMFALVLMSIAFLLYASGLTITLYIATGLTGISYGVLYSM 480

Query: 481 MVPLASEIFGLKNFGVIFNFMQLGNPIGAVLFSVLLASTLYDNEAAKQGSITCIGQQCFR 540
           MVPLASEIFGLKNFGVIFNFMQLGNPIGAVLFSVLL STLYD EAA QGSITCIGQQCFR
Sbjct: 481 MVPLASEIFGLKNFGVIFNFMQLGNPIGAVLFSVLLTSTLYDKEAAMQGSITCIGQQCFR 540

Query: 541 TTFFILSGVAGLGSIVSMILTVRLRPVYQMLYSGGSFRLPQSSGH 586
           TTFFILSGVAGLGSIVS+ILT+RLRPVYQMLY+GGSFRLPQSSGH
Sbjct: 541 TTFFILSGVAGLGSIVSLILTIRLRPVYQMLYAGGSFRLPQSSGH 565

BLAST of CaUC07G136690 vs. ExPASy TrEMBL
Match: A0A6J1GWT3 (protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucurbita moschata OX=3662 GN=LOC111457872 PE=4 SV=1)

HSP 1 Score: 983.8 bits (2542), Expect = 3.1e-283
Identity = 517/586 (88.23%), Postives = 548/586 (93.52%), Query Frame = 0

Query: 1   MPKLVLKGGSRPPWVGLAAAVWVQIAGGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI 60
           M KLVLKGGSRPPWVGLAAAVWVQIA GSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI
Sbjct: 1   MSKLVLKGGSRPPWVGLAAAVWVQIAAGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI 60

Query: 61  GESMGLVPGLACNRFPPWVILLFGAFCCFLGYGAIWLAVSRTVPNLPYWLVSLMFIFSLC 120
           GES+GL+PGLACNRFPPW +LL GA CCF+GYGAIWLAVSRTVPNLPYW           
Sbjct: 61  GESVGLLPGLACNRFPPWAVLLVGAVCCFVGYGAIWLAVSRTVPNLPYW----------- 120

Query: 121 EDLSLIFLSVLLAADFHELLSNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYT 180
               L++L+  +A +     SNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYT
Sbjct: 121 ----LLWLTHCIATN-----SNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYT 180

Query: 181 VIYSMVLKKSALNLLLFLAIGIPILCLALMYFVRPCTPASSEDPSERAHFLFTQAACVLL 240
           VIYSMVL+KSAL+L+LFLAIGIPILCLALMYFVRPCTPAS+EDPSER HFLFTQAACVLL
Sbjct: 181 VIYSMVLRKSALDLVLFLAIGIPILCLALMYFVRPCTPASTEDPSERNHFLFTQAACVLL 240

Query: 241 AVFLVSTTILDATTTPSDAVGYTLVAIMVILLMSPLAVPIKMTICA-KSKKLGPRVDSAE 300
           AVFLVSTTILDATTTPS+AV YTLVAIMV+LLMSPLAVPIKMTIC+ K+KKLGPRVDS E
Sbjct: 241 AVFLVSTTILDATTTPSNAVAYTLVAIMVVLLMSPLAVPIKMTICSVKTKKLGPRVDSTE 300

Query: 301 PLASGESDSSQIEPLLTPSSSATNLGSFCENDDSSDVETLLAVGEGAIHKKKRRPKRGED 360
           PLASGESDSSQIEPLLTPSSSATNLGSF END+SSDVETLLAVGEGAIHKKKR+PKRGED
Sbjct: 301 PLASGESDSSQIEPLLTPSSSATNLGSFYENDESSDVETLLAVGEGAIHKKKRKPKRGED 360

Query: 361 FKLREAVIKADFWLLWFLYFLGVGPGITILNNLSQIGISLGVNDTTLLLALFSFCNFVGR 420
           FKLREAVIKADFWLLWFLYFLGVGPGIT+LNNLSQIGI+LGVNDTTLLLALFSFCNFVGR
Sbjct: 361 FKLREAVIKADFWLLWFLYFLGVGPGITVLNNLSQIGIALGVNDTTLLLALFSFCNFVGR 420

Query: 421 LGSGVISEHFVRSRMIPRSLWMMFALILMAIAFLLYASALTGTLYVATGLTGISYGVLYS 480
           LGSGVISEHFVRSRMIPRSLWMMFAL+LM++AFLLYASA T TLYVATGL GISYGVLY+
Sbjct: 421 LGSGVISEHFVRSRMIPRSLWMMFALMLMSVAFLLYASAHTVTLYVATGLMGISYGVLYA 480

Query: 481 MMVPLASEIFGLKNFGVIFNFMQLGNPIGAVLFSVLLASTLYDNEAAKQGSITCIGQQCF 540
           MMVPLASEIFGLKNFGVIFNFMQLGNPIGAVLFSVLLASTLYDNEAAKQGSITCIGQ+CF
Sbjct: 481 MMVPLASEIFGLKNFGVIFNFMQLGNPIGAVLFSVLLASTLYDNEAAKQGSITCIGQECF 540

Query: 541 RTTFFILSGVAGLGSIVSMILTVRLRPVYQMLYSGGSFRLPQSSGH 586
           R TFF+LSGVAGLGSI+ +ILTVRLRPVYQMLY+GGSFRLPQ+SGH
Sbjct: 541 RVTFFVLSGVAGLGSILCIILTVRLRPVYQMLYAGGSFRLPQNSGH 566

BLAST of CaUC07G136690 vs. TAIR 10
Match: AT1G80530.1 (Major facilitator superfamily protein )

HSP 1 Score: 641.0 bits (1652), Expect = 9.6e-184
Identity = 335/586 (57.17%), Postives = 432/586 (73.72%), Query Frame = 0

Query: 1   MPKLVLKGGSRPPWVGLAAAVWVQIAGGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDI 60
           M KL  K GSRPPWVGLAAA WVQ++ GS   F LYS  LKSVLG +QQQ+T+LGVA D+
Sbjct: 1   MTKLAEKSGSRPPWVGLAAAAWVQVSAGSGSTFPLYSSALKSVLGFSQQQVTILGVACDL 60

Query: 61  GESMGLVPGLACNRFPPWVILLFGAFCCFLGYGAIWLAVSRTVPNLPYWLVSLMFIFSLC 120
           GE+MGL+PG A N+ PPW +LL GA  CFLG+G +WL+VS+ V  LP+W           
Sbjct: 61  GENMGLLPGYASNKLPPWSMLLIGASSCFLGFGVLWLSVSQIVLGLPFW----------- 120

Query: 121 EDLSLIFLSVLLAADFHELLSNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYT 180
               L+F+++ LA +     SN+WFGTA LVTNMRNFP+SRG VAG+LKGY+G+S A +T
Sbjct: 121 ----LLFVALALATN-----SNSWFGTASLVTNMRNFPMSRGPVAGLLKGYIGISGAAFT 180

Query: 181 VIYSMVLKKSALNLLLFLAIGIPILCLALMYFVRPCTPASSEDPSERAHFLFTQAACVLL 240
           V++SMVL  SA++LLLFL +GIP++CL +MYF+RPC PA+ EDPSE  +F F     +L 
Sbjct: 181 VLFSMVLHHSAMDLLLFLTVGIPVICLTVMYFIRPCIPATGEDPSEPMYFAFLLVTSILF 240

Query: 241 AVFLVSTTILDATTTPSDAVGYTLVAIMVILLMSPLAVPIKMTICAKSKKLGPRVDSAEP 300
           A +LV TT+L         + Y LVAIMV+LL+SPLAVPIKMT+   + K  P + S++ 
Sbjct: 241 AAYLVVTTVLSEVFILPSILKYVLVAIMVLLLLSPLAVPIKMTLFRSNAKSSP-LGSSDN 300

Query: 301 LASGESDSSQIEPLLTPSSSATNLGSFCENDDSSDVETLLAVGEGAIHKKKRRPKRGEDF 360
           LA  + + +  EPLLTPS+SA+NLG   E DD SD+E LLA  EGA+ KKKR+P+RGEDF
Sbjct: 301 LA--KEEGTHEEPLLTPSTSASNLGPIFEGDDESDMEILLAEAEGAV-KKKRKPRRGEDF 360

Query: 361 KLREAVIKADFWLLWFLYFLGVGPGITILNNLSQIGISLGVNDTTLLLALFSFCNFVGRL 420
           K  +  +KADFWLLWF+YFLG+G G+T+ NNL+QIG + G+ DTT+LL LFSF NF+GRL
Sbjct: 361 KFGQVFVKADFWLLWFVYFLGMGSGVTVSNNLAQIGFAFGIKDTTILLCLFSFFNFIGRL 420

Query: 421 GSGVISEHFVRSRMIPRSLWMMFALILMAIAFLLYASALTGTLYVATGLTGISYGVLYSM 480
            SG ISEHFVRSR +PR+LWM  A ++M   FLL+A A+  T+YVAT L GI  G  + +
Sbjct: 421 ASGAISEHFVRSRTLPRTLWMGAAQLVMVFTFLLFAMAIDHTIYVATALIGICMGFQF-L 480

Query: 481 MVPLASEIFGLKNFGVIFNFMQLGNPIGAVLFSVLLASTLYDNEAAKQGSITCIGQQCFR 540
            +   SE+FGL++FG+ FNF+ LGNP+GA +FS +LA  +YD EA KQG +TCIG  CFR
Sbjct: 481 SIATISELFGLRHFGINFNFILLGNPLGATIFSAILAGYIYDKEADKQGKMTCIGPDCFR 540

Query: 541 TTFFILSGVAGLGSIVSMILTVRLRPVYQMLYSGGSFRL-PQSSGH 586
            TF +L+GV GLG+++S+ILTVR+RPVYQ LY+ GSFRL PQS+GH
Sbjct: 541 VTFLVLAGVCGLGTLLSIILTVRIRPVYQALYASGSFRLQPQSTGH 561

BLAST of CaUC07G136690 vs. TAIR 10
Match: AT4G34950.1 (Major facilitator superfamily protein )

HSP 1 Score: 332.4 bits (851), Expect = 7.3e-91
Identity = 215/583 (36.88%), Postives = 329/583 (56.43%), Query Frame = 0

Query: 14  WVGLAAAVWVQIAGGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDIGESMGLVPGLACN 73
           W+G   AVWVQ   G++Y FS YS  LKS++ L Q +L  L VA D+G++ G++ GLA +
Sbjct: 17  WLGFVTAVWVQSISGNNYTFSNYSGALKSLMNLTQLELNSLSVAKDVGKAFGILAGLASD 76

Query: 74  RFPPWVILLFGAFCCFLGYGAIWLAVSRTVPNLPYWLVSLMFIFSLCEDLSLIFLSVLLA 133
           R    VILL G+F   LGYG  WL VSRT+  +PYW    M +F LC   +         
Sbjct: 77  RLSTPVILLIGSFEGLLGYGVQWLVVSRTIQPIPYW---QMCVF-LCMGGN--------- 136

Query: 134 ADFHELLSNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYTVIYSMVLKKSALN 193
                  S  W  TAVLVT +RNF  +RG V+GILKGYVGLS A++T + + +      +
Sbjct: 137 -------STTWMNTAVLVTCIRNFRRNRGPVSGILKGYVGLSTAIFTDLCNALFSSDPAS 196

Query: 194 LLLFLAIGIPILCLALMYFVRPCTPAS--SEDPSERAHFLFTQAACVLLAVFLVSTTILD 253
            L+ L++    +CL  ++F+R   P++  +ED  E  +F       V++AV+L S  I+ 
Sbjct: 197 FLVLLSVVPFAVCLTAVFFLREIPPSTTFAEDNEESKYFAVFNIVAVVVAVYLQSYDIIG 256

Query: 254 ATTTPSDAVGYTLVAIMVILLMSPLAVPIKMTICAK---SKKLGPRVDSAEPLASGESDS 313
             T    A      +I++ILL SP+AVP    I +K    + +  R+D  EPL    S+ 
Sbjct: 257 IKT---GAFSIAFASILLILLASPVAVPFHAFIRSKVHDEQDVEGRID--EPLLRSGSEI 316

Query: 314 SQIEPLLTPSSSATN----LGSFCENDDSSDVETLLAVGEGAIHKKKRRPKRGEDFKLRE 373
              E ++  +++A N          N++  +  T++         +K+RP  GE+  + E
Sbjct: 317 EVEETIVGAAAAADNELPPSLKPLSNEEEENHGTIVTT-------EKKRPVLGEEHTIME 376

Query: 374 AVIKADFWLLWFLYFLGVGPGITILNNLSQIGISLGVNDTTLLLALFSFCNFVGRLGSGV 433
           A++  DFW+L+  +  GVG G+ ++NN+ QIG++LG  D ++ +++ S   F GR+ SG 
Sbjct: 377 AMLTVDFWVLFVSFLCGVGTGLAVMNNMGQIGLALGYTDVSIFVSMTSIWGFFGRILSGT 436

Query: 434 ISEHFVRSRMIPRSLWMMFALILMAIAFLLYASALTGTLYVATGLTGISYGVLYSMMVPL 493
           ISEHF++    PR LW   A I+MA+ +LL A AL G+LY+ + + G+ YGV  ++ VP 
Sbjct: 437 ISEHFIKKAGTPRPLWNAAAQIIMAVGYLLMALALPGSLYIGSMVVGVCYGVRLAITVPT 496

Query: 494 ASEIFGLKNFGVIFNFMQLGNPIGAVLFSVLLASTLYDNEAAKQ--GSITCIGQQCFRTT 553
           ASE+FGLK +G+I+N + L  P+G+ LFS LLA  LYD EA     G  TC+G  CFR  
Sbjct: 497 ASELFGLKYYGLIYNILILNMPLGSFLFSGLLAGLLYDAEATPTPGGGNTCVGAHCFRIV 556

Query: 554 FFILSGVAGLGSIVSMILTVRLRPVYQMLY-------SGGSFR 579
           F +++  + +G  + ++L  R + +Y  ++       SGG+ R
Sbjct: 557 FIVMAFASIIGVGLDLLLAYRTKGIYAKIHASKKTKKSGGNLR 567

BLAST of CaUC07G136690 vs. TAIR 10
Match: AT2G16660.1 (Major facilitator superfamily protein )

HSP 1 Score: 329.7 bits (844), Expect = 4.7e-90
Identity = 214/582 (36.77%), Postives = 319/582 (54.81%), Query Frame = 0

Query: 6   LKGGSRPPWVGLAAAVWVQIAGGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDIGESMG 65
           +   S   W+G   AVWVQ   G++Y FS YS  LKS++ LNQ +L  L VA D+G++ G
Sbjct: 7   ISSSSALKWLGFVTAVWVQSISGNNYTFSNYSGALKSLMNLNQLELNNLSVAKDVGKAFG 66

Query: 66  LVPGLACNRFPPWVILLFGAFCCFLGYGAIWLAVSRTVPNLPYWLVSLMFIFSLCEDLSL 125
           ++ GLA +R P  VILL G F   LGYG  WL VSRT+  +PYW    M IF LC   + 
Sbjct: 67  ILAGLASDRLPTPVILLIGCFEGLLGYGVQWLVVSRTIQPIPYW---QMCIF-LCMGGN- 126

Query: 126 IFLSVLLAADFHELLSNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYTVIYSM 185
                          S  W  TAVLVT +RNF  +RG V+GILKGYVGLS A++T + + 
Sbjct: 127 ---------------STTWMNTAVLVTCIRNFRRNRGPVSGILKGYVGLSTAIFTDLCTA 186

Query: 186 VLKKSALNLLLFLAIGIPILCLALMYFVRPCTPASS--EDPSERAHFLFTQAACVLLAVF 245
           +      + L+ LA+    +CL  ++F+R   PASS  E+  E  +F       V++AV+
Sbjct: 187 LFSNDPASFLVLLAVVPFAVCLTAVFFLREIPPASSAAEENEETRYFTIFNIVAVVVAVY 246

Query: 246 LVSTTILDATTTPSDAVGYTLVAIMVILLMSPLAVPIKMTICAKSKKLGPRVDSAEPLAS 305
           L S  I+   T           +I++ LL SP+A+P    I  KS   G + D    +  
Sbjct: 247 LQSYDIIGIKT---GVFSVAFASILLFLLASPIAIPFHSFI--KSLNYGEQDDLEGRIQ- 306

Query: 306 GESDSSQIEPLLTPSSSATNLGSFCENDDSSDVETLLAVGEGAIHKKKRRPKRGEDFKLR 365
                   EPLL    +A             +V  + AV      KK+++P  GED  + 
Sbjct: 307 --------EPLLRSEIAAA----------EKEVIVVAAVAAEEEEKKRKKPVLGEDHTIM 366

Query: 366 EAVIKADFWLLWFLYFLGVGPGITILNNLSQIGISLGVNDTTLLLALFSFCNFVGRLGSG 425
           EAV+  DFW+L+  +  GVG G+ ++NN+ QIG++LG  + ++ +++ S   F GR+ SG
Sbjct: 367 EAVLTVDFWVLFVSFLCGVGTGLAVMNNMGQIGLALGYTNVSIFVSMTSIWGFFGRILSG 426

Query: 426 VISEHFVRSRMIPRSLWMMFALILMAIAFLLYASALTGTLYVATGLTGISYGVLYSMMVP 485
            +SE+F++    PR LW   + ILMA+ ++L A A+  +LY+ + + G+ YGV  ++ VP
Sbjct: 427 TLSEYFLKKAGTPRPLWNAASQILMAVGYILMALAVPNSLYIGSMVVGVCYGVRLAITVP 486

Query: 486 LASEIFGLKNFGVIFNFMQLGNPIGAVLFSVLLASTLYDNEAAKQ--GSITCIGQQCFRT 545
            ASE+FGLK +G+I+N + L  P+G+ LFS LLA  LYD EA     G  TC+G  C+R 
Sbjct: 487 TASELFGLKYYGLIYNILVLNLPLGSFLFSGLLAGFLYDAEATPTPGGGNTCVGAHCYRL 544

Query: 546 TFFILSGVAGLGSIVSMILTVRLRPVYQMLY-------SGGS 577
            F +++  + +G  + ++L  R + +Y  ++       SGGS
Sbjct: 547 IFIVMALASVIGVGLDLVLAYRTKEIYAKIHASKKSKKSGGS 544

BLAST of CaUC07G136690 vs. TAIR 10
Match: AT5G14120.1 (Major facilitator superfamily protein )

HSP 1 Score: 318.2 bits (814), Expect = 1.4e-86
Identity = 201/586 (34.30%), Postives = 315/586 (53.75%), Query Frame = 0

Query: 14  WVGLAAAVWVQIAGGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDIGESMGLVPGLACN 73
           W+   AA+W+Q   G  Y F   S ++KS L  NQ++L+ LGVA D+G+S+G + G    
Sbjct: 17  WLVFVAAMWIQSCAGIGYLFGSISPVIKSSLNYNQKELSRLGVAKDLGDSVGFIAGTLSE 76

Query: 74  RFPPWVILLFGAFCCFLGYGAIWLAVSRTVPNLPYWLVSLMFIFSLCEDLSLIFLSVLLA 133
             P W  LL GA    +GYG +WL V+   P LP W + +           LIF+     
Sbjct: 77  ILPLWAALLVGAVQNLIGYGWVWLIVTGRAPILPLWAMCV-----------LIFVG---- 136

Query: 134 ADFHELLSNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYTVIYSMVLKKSALN 193
                     +F T  LV+ ++NFP SRG V GILKG+ GL  A+ + IY+M+   +  +
Sbjct: 137 -----NNGETYFNTGALVSGVQNFPKSRGPVVGILKGFAGLGGAIISQIYTMIHSSNPAS 196

Query: 194 LLLFLAIGIPILCLALMYFVRPCTPASSEDPSERAHFLFTQAACVLLAVFLVSTTILDAT 253
           L+L +A+   ++ + LM+F+RP        P++ A F F    C+LLA +L+S  ++   
Sbjct: 197 LILMVAVTPAVVVVCLMFFIRPVGGHKQIRPTDGASFTFIYGVCLLLAAYLMSVMLIQDL 256

Query: 254 TTPSDAVGYTLVAIMVILLMSPLAVPIKMTICAKSKKLGPRVDSAEPLASGESDSSQIEP 313
              S  V      ++ ++L+ P+ VPI  +   ++ +    ++  EPL     D  Q   
Sbjct: 257 VVVSHNVITVFTIVLFVILVVPILVPIMTSFFTETNEPDDTIE--EPLVPKRED--QEPG 316

Query: 314 LLTPSSSATNLGSFCENDDSSDVETL-----------------LAVGEGAIHKKKRR-PK 373
           L TP    + +    E++   DV+ L                  A  EGA+   +RR P 
Sbjct: 317 LQTPDLILSEV----EDEKPKDVDLLPASERHKRIAHLQAQLMQAAAEGAVRVNRRRGPH 376

Query: 374 RGEDFKLREAVIKADFWLLWFLYFLGVGPGITILNNLSQIGISLGVNDTTLLLALFSFCN 433
           RGEDF L +A++KADFWL++F   LG G G+T+++NL Q+  SLG ++T +L+++ S  N
Sbjct: 377 RGEDFTLTQALVKADFWLIFFSLLLGSGSGLTVIDNLGQMSQSLGYDNTHVLVSMISIWN 436

Query: 434 FVGRLGSGVISEHFVRSRMIPRSLWMMFALILMAIAFLLYASALTGTLYVATGLTGISYG 493
           F+GR+G G  SE  VR    PR + M  A ++M++  + +A    G +Y+ T L G+ YG
Sbjct: 437 FLGRIGGGYFSELVVRDYAYPRPVAMAVAQLIMSVGHIFFAYGWPGAMYIGTLLIGLGYG 496

Query: 494 VLYSMMVPLASEIFGLKNFGVIFNFMQLGNPIGAVLFSVLLASTLYDNEAAKQG------ 553
             ++++   ASE+FGLK FG ++NF+ L NP G+++FS ++AS++YD EA +Q       
Sbjct: 497 AHWAIVPATASELFGLKKFGALYNFLTLANPAGSLVFSGMIASSIYDREAERQAHGSVFD 556

Query: 554 ---SITCIGQQCFRTTFFILSGVAGLGSIVSMILTVRLRPVYQMLY 573
              ++ C G  CF  T  I+SG   +  ++SMIL  R + VY  LY
Sbjct: 557 PDDALRCNGSICFFLTSLIMSGFCIIACMLSMILVRRTKSVYTHLY 574

BLAST of CaUC07G136690 vs. TAIR 10
Match: AT3G01930.2 (Major facilitator superfamily protein )

HSP 1 Score: 309.3 bits (791), Expect = 6.6e-84
Identity = 194/586 (33.11%), Postives = 309/586 (52.73%), Query Frame = 0

Query: 14  WVGLAAAVWVQIAGGSSYNFSLYSHLLKSVLGLNQQQLTVLGVANDIGESMGLVPGLACN 73
           W+   AA+W+Q   G  Y F   S ++KS L  NQ+QL+ LGVA D+G+S+G + G    
Sbjct: 17  WLVFVAAMWIQSCAGIGYLFGSISPVIKSSLNYNQKQLSRLGVAKDLGDSVGFLAGTLSE 76

Query: 74  RFPPWVILLFGAFCCFLGYGAIWLAVSRTVPNLPYWLVSLMFIFSLCEDLSLIFLSVLLA 133
             P W  LL G+    +GYG +WL V+   P LP W + +           LIF+     
Sbjct: 77  ILPLWAALLVGSVQNLVGYGWVWLIVTGRAPILPLWAMCI-----------LIFVG---- 136

Query: 134 ADFHELLSNAWFGTAVLVTNMRNFPLSRGTVAGILKGYVGLSAAVYTVIYSMVLKKSALN 193
                     +F TA LV+ ++NFP SRG V GILKG+ GL  A+ + +Y+M+      +
Sbjct: 137 -----NNGETYFNTAALVSGVQNFPKSRGPVVGILKGFAGLGGAILSQVYTMIHSSDRAS 196

Query: 194 LLLFLAIGIPILCLALMYFVRPCTPASSEDPSERAHFLFTQAACVLLAVFLVSTTILDAT 253
           L+  +A+   ++ + LM+F+RP         S+   F    A C+LLA +L++  +++  
Sbjct: 197 LIFMVAVAPSVVVVPLMFFIRPVGGHRQIRSSDATSFTVIYAVCILLAAYLMAVMLVEDF 256

Query: 254 TTPSDAVGYTLVAIMVILLMSPLAVPIKMTICAKSKKLGPRVDSAEPLASGESDSSQIEP 313
              S ++      ++  +L+ P+ +PI  +    S       D+ E    G+        
Sbjct: 257 IDLSHSIIIAFTVVLFAILLVPIFIPIATSCFTAST---DPCDTLEEPLLGDQQGQDPGQ 316

Query: 314 LLTPSSSATNLGSFCENDDSSDVETLLAV-----------------GEGAIHKKKRR-PK 373
             TP      + S  E++   +V+ L AV                  EGA+  K+RR P 
Sbjct: 317 STTPDHGPELIFSEVEDEKPKEVDLLPAVERHKRIAQLQAKLMQAAAEGAVRVKRRRGPH 376

Query: 374 RGEDFKLREAVIKADFWLLWFLYFLGVGPGITILNNLSQIGISLGVNDTTLLLALFSFCN 433
           RGEDF L +A++KADFWL++F   LG G G+T+++NL Q+  SLG ++T + +++ S  N
Sbjct: 377 RGEDFTLTQALVKADFWLIFFSLLLGSGSGLTVIDNLGQMSQSLGYDNTHVFVSMISIWN 436

Query: 434 FVGRLGSGVISEHFVRSRMIPRSLWMMFALILMAIAFLLYASALTGTLYVATGLTGISYG 493
           F+GR+G G  SE  VR    PR + +  A ++M++  + +A    G +++ T L G+ YG
Sbjct: 437 FLGRIGGGYFSELIVRDYAYPRPVAIAVAQLVMSVGHIFFAYGWPGAMHIGTLLIGLGYG 496

Query: 494 VLYSMMVPLASEIFGLKNFGVIFNFMQLGNPIGAVLFSVLLASTLYDNEAAKQGS----- 553
             ++++   ASE+FGLK FG ++NF+ L NP G+++FS L+AS++YD EA +Q       
Sbjct: 497 AHWAIVPATASELFGLKKFGALYNFLTLANPAGSLVFSGLIASSIYDREAERQAQGSLFN 556

Query: 554 ----ITCIGQQCFRTTFFILSGVAGLGSIVSMILTVRLRPVYQMLY 573
               + C G  C+  T  I+SG   + + +SMIL  R +PVY  LY
Sbjct: 557 PDDVLRCRGSICYFLTSLIMSGFCLIAAALSMILVQRTKPVYTNLY 579

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038896285.12.4e-29391.79protein NUCLEAR FUSION DEFECTIVE 4 isoform X1 [Benincasa hispida][more]
XP_004152187.12.0e-29290.60protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis sativus] >KGN52923.1 hypothetical pr... [more]
TYK29592.19.9e-29290.60protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis melo var. makuwa][more]
XP_008454196.22.9e-29190.60PREDICTED: LOW QUALITY PROTEIN: protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis melo... [more]
KAA0044465.12.4e-29090.26protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
F4I9E11.1e-4830.27Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 ... [more]
Q5AXV12.0e-0522.14Probable transporter mch1 OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / ... [more]
Match NameE-valueIdentityDescription
A0A0A0KTP39.7e-29390.60Nodulin-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G00630... [more]
A0A5D3E0Y74.8e-29290.60Protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3BY601.4e-29190.60LOW QUALITY PROTEIN: protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo OX=3656 ... [more]
A0A5A7TSX41.2e-29090.26Protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C... [more]
A0A6J1GWT33.1e-28388.23protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucurbita moschata OX=3662 GN=LOC111457872... [more]
Match NameE-valueIdentityDescription
AT1G80530.19.6e-18457.17Major facilitator superfamily protein [more]
AT4G34950.17.3e-9136.88Major facilitator superfamily protein [more]
AT2G16660.14.7e-9036.77Major facilitator superfamily protein [more]
AT5G14120.11.4e-8634.30Major facilitator superfamily protein [more]
AT3G01930.26.6e-8433.11Major facilitator superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (USVL246-FR2) v1
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR010658Nodulin-likePFAMPF06813Nodulin-likecoord: 13..280
e-value: 5.6E-79
score: 265.2
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 366..568
e-value: 2.5E-11
score: 45.4
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 11..219
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 352..566
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 294..318
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 302..318
NoneNo IPR availablePANTHERPTHR21576UNCHARACTERIZED NODULIN-LIKE PROTEINcoord: 3..585
NoneNo IPR availablePANTHERPTHR21576:SF97MAJOR FACILITATOR SUPERFAMILY PROTEINcoord: 3..585
NoneNo IPR availableCDDcd17354MFS_Mch1p_likecoord: 12..553
e-value: 8.88482E-101
score: 308.797

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CaUC07G136690.1CaUC07G136690.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0022857 transmembrane transporter activity