CaUC07G136570 (gene) Watermelon (USVL246-FR2) v1

Overview
NameCaUC07G136570
Typegene
OrganismCitrullus amarus (Watermelon (USVL246-FR2) v1)
DescriptionCUE domain-containing protein
LocationCiama_Chr07: 26773007 .. 26775609 (+)
RNA-Seq ExpressionCaUC07G136570
SyntenyCaUC07G136570
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TCTTTTAAAATCCCATGTAACGCTCCTCTCTGTAAAATGGGAATGCATTAGGAAAGGGGAAAGCAAAGACTTTAAAAGAACCTAACCCCAAAGAAATCAAATTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTTCCCAAGCAATCTCCTTAAACCCTAACCTCTTCCCTTTCTGATTTGCATCGGATTTGAATTTGGTGTTTGCTCCGTGGACGAAGGCCTTCTTTAGGAATTAGCTTCTGCAAGCCACTACCCGGAGACCATGTCTGCTGTGGTGTGCGGTACCAAGAGATCCTTCTTTGAAGAACTCCCGCCTTCCCCTCCCATTGCCAAGCGCCTCCGCTGCTCCACTTCTACTTCTCCGATTCCATTCGCTGCCCCTTCTCATGTTGATCACCTCCACCATTTGTTTCCCCACATGGACCATCAGGTTTCTTCTTTCTTCCTTTTGTTTTTAAATTAACTTTCGCTTCAATTTGATAAACTGTCGTACTGAGATTGCTGGTTGTTTTTGTTTCCTGCGTTGCTTAGTTCCTGCTTGTGTGTAATTTGATACTTCTTGATAGAATAGGATTATAAACTGAATCTATATGGAACTTTTTACAATAGTTTTGGACCTTGGGGGGGAATCATGTTATTCTCTTCTTTTGGATTGTGATTTACCAACTAACTTGAATTAGTTGCGGTATGAAATTGGAGTGGTTCTATTCAGTTTTGATTTGTAATGCATCTCTTTCGGTGTCTTGTTAGATAACTGCTGCACATGTATGTTATAATTAATATTAAGTTTGTTTTTTCCCCAAGGTTTTCTTTATCATGCTTTGATTTTGCCTTTCAGCTTCTTGTGAGAGCCCTTGAAGAATGTGGCAACGACTTGGATGCTGCCATCAGAAGTCTGAGTGACCTGTGTTTGGGGTCTGCTGTTGAGAATCCTGTTACTACTGCAGAACCAGAGAAAAATTTGGATCAAGGTATCACTGAGTGCTAAGTAATGAAGGAGATGCTTCATATGTAGTTCCTTGTTTCTGTTGGCTCATGATTTTAATTATATTATGACGATGAATTTCTTACATTCAAGATTAAATTCCCCCATCAAGGATTTGATGCATGTTTATTTCATAGAAGAGATCTAATGGGTCCCCGTCCCTATAACTTGCTGTCCCTAGTTTGGAATCCTAATTATACCCCTGCCTTGTGCAGGTTAGAGGAAGCTTTAAGTTTTGATTGAAATTTGAAAAACAAGAAACAGAGTTCTCATGCAGAGTACTATGCATCCATTATATTAGAATAAGATGTATTCTTTTCTCGTGTAAGTGACTAAATGCTTTGCTACCTACCCTCCTGTTTGTATTTTTGGTTGATAAGCTTATGTTTACGAAATTGAATTGGCTGGTACATATACATAAACAGTTGGAAAAAAACCTTAAATCTGAATTTCTGGAGATAAAGCTGTATGAGGAGTTATAATCTTCTTTAATTCTTTTGTTATTGATGCTGTATTGCCCCTGATCGTTTTGTAATGTGCTTTTTTCTAGCCTGGACATTTATTTCTAGCTTTTCAGCACTATCTATCGTAATACGTTCTTTTTGGTTCTTTTGATGAAAATACATCTCTGCTTTTTATTAAAAAAAAGCACTATCTATTGTAAAACTTTGATATTAATGTTGAACTTATCATACAGCTTTTCTGCTGAAAGCACCGTTCTTAACCTTTTGTTGTTAGCCTTGAGCTTTTATCTTATTTCTTTTCTGAAAACATCAGTAATTCAGTGCACGTTTTATAAATTTGCAGGTTCATTTGCTAACAATGGAGAGGCTGCTGCTTCTGTGAATTGTTCAGCACCAAGCAGTGTTTCTCTTGATGGTAGAAAATGGATTGACTTGTTTGTTGTGGAAATGATGAACGCTACCACGATAGCTGATGCTAAGACTCGTGCAGCAAGAGCACTAGAGGCATTAGAGAACTCAATTAGTGAACATGCTGGTGTTGATGCTGCAAAAAATTTCCACAAGGTACTTCAATTTTAGTGCCCACAACTCTAAAAAAGTTTCCACCTCCTTGGGGGACAATTTTTGGATACTAATATGTTTTCGGAGCACTCGAAAGCAGTTAGATTTGGTTTGGGCTTATTTGCTTGTTAAACATGCTATTGCTATAAAGTAATGATCCAGGCTTGTAAACTTATGTTCTTTTCAATCTCACTCAGGAAAATACGCAACTGAAGGAACAAATTGATGTACTTCTACGAGAAAATACAATTCTTAAGCGGGCGGTTGCCATACAACATGAGCGTCAGAAGGAATTTGAGGATAAGAATCTAGAGTTGCAGCACCTTAAACAGATGGTATCTCAATATCAGGAGCAGCTCAGAACCCTAGAGGTCAGAATAAATGTAGAACTATTTTAAGTTCTTCCCCAGTGTTAACTGACCTGTTTTAATTTCTTAAACACGTTCATGTTGATGCAACTTTTCTGACCAGTTGAACAAAAATCCTGTCAATTTGTTACAGATAAACAATTATGCATTGACAATGCATTTGAAGCAGGCTCAACAAAGTAGCTCGATTCCAGGACGCTTCCATCCCGATGTCTTCTAG

mRNA sequence

TCTTTTAAAATCCCATGTAACGCTCCTCTCTGTAAAATGGGAATGCATTAGGAAAGGGGAAAGCAAAGACTTTAAAAGAACCTAACCCCAAAGAAATCAAATTCTTCTTCTTCTTCTCTTTCCCAAGCAATCTCCTTAAACCCTAACCTCTTCCCTTTCTGATTTGCATCGGATTTGAATTTGGTGTTTGCTCCGTGGACGAAGGCCTTCTTTAGGAATTAGCTTCTGCAAGCCACTACCCGGAGACCATGTCTGCTGTGGTGTGCGGTACCAAGAGATCCTTCTTTGAAGAACTCCCGCCTTCCCCTCCCATTGCCAAGCGCCTCCGCTGCTCCACTTCTACTTCTCCGATTCCATTCGCTGCCCCTTCTCATGTTGATCACCTCCACCATTTGTTTCCCCACATGGACCATCAGCTTCTTGTGAGAGCCCTTGAAGAATGTGGCAACGACTTGGATGCTGCCATCAGAAGTCTGAGTGACCTGTGTTTGGGGTCTGCTGTTGAGAATCCTGTTACTACTGCAGAACCAGAGAAAAATTTGGATCAAGGTTCATTTGCTAACAATGGAGAGGCTGCTGCTTCTGTGAATTGTTCAGCACCAAGCAGTGTTTCTCTTGATGGTAGAAAATGGATTGACTTGTTTGTTGTGGAAATGATGAACGCTACCACGATAGCTGATGCTAAGACTCGTGCAGCAAGAGCACTAGAGGCATTAGAGAACTCAATTAGTGAACATGCTGGTGTTGATGCTGCAAAAAATTTCCACAAGGAAAATACGCAACTGAAGGAACAAATTGATGTACTTCTACGAGAAAATACAATTCTTAAGCGGGCGGTTGCCATACAACATGAGCGTCAGAAGGAATTTGAGGATAAGAATCTAGAGTTGCAGCACCTTAAACAGATGGTATCTCAATATCAGGAGCAGCTCAGAACCCTAGAGATAAACAATTATGCATTGACAATGCATTTGAAGCAGGCTCAACAAAGTAGCTCGATTCCAGGACGCTTCCATCCCGATGTCTTCTAG

Coding sequence (CDS)

ATGTCTGCTGTGGTGTGCGGTACCAAGAGATCCTTCTTTGAAGAACTCCCGCCTTCCCCTCCCATTGCCAAGCGCCTCCGCTGCTCCACTTCTACTTCTCCGATTCCATTCGCTGCCCCTTCTCATGTTGATCACCTCCACCATTTGTTTCCCCACATGGACCATCAGCTTCTTGTGAGAGCCCTTGAAGAATGTGGCAACGACTTGGATGCTGCCATCAGAAGTCTGAGTGACCTGTGTTTGGGGTCTGCTGTTGAGAATCCTGTTACTACTGCAGAACCAGAGAAAAATTTGGATCAAGGTTCATTTGCTAACAATGGAGAGGCTGCTGCTTCTGTGAATTGTTCAGCACCAAGCAGTGTTTCTCTTGATGGTAGAAAATGGATTGACTTGTTTGTTGTGGAAATGATGAACGCTACCACGATAGCTGATGCTAAGACTCGTGCAGCAAGAGCACTAGAGGCATTAGAGAACTCAATTAGTGAACATGCTGGTGTTGATGCTGCAAAAAATTTCCACAAGGAAAATACGCAACTGAAGGAACAAATTGATGTACTTCTACGAGAAAATACAATTCTTAAGCGGGCGGTTGCCATACAACATGAGCGTCAGAAGGAATTTGAGGATAAGAATCTAGAGTTGCAGCACCTTAAACAGATGGTATCTCAATATCAGGAGCAGCTCAGAACCCTAGAGATAAACAATTATGCATTGACAATGCATTTGAAGCAGGCTCAACAAAGTAGCTCGATTCCAGGACGCTTCCATCCCGATGTCTTCTAG

Protein sequence

MSAVVCGTKRSFFEELPPSPPIAKRLRCSTSTSPIPFAAPSHVDHLHHLFPHMDHQLLVRALEECGNDLDAAIRSLSDLCLGSAVENPVTTAEPEKNLDQGSFANNGEAAASVNCSAPSSVSLDGRKWIDLFVVEMMNATTIADAKTRAARALEALENSISEHAGVDAAKNFHKENTQLKEQIDVLLRENTILKRAVAIQHERQKEFEDKNLELQHLKQMVSQYQEQLRTLEINNYALTMHLKQAQQSSSIPGRFHPDVF
Homology
BLAST of CaUC07G136570 vs. NCBI nr
Match: XP_023528958.1 (uncharacterized protein LOC111791720 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 448.7 bits (1153), Expect = 3.3e-122
Identity = 230/261 (88.12%), Postives = 245/261 (93.87%), Query Frame = 0

Query: 1   MSAVVCGTKRSFFEELPPSPPIAKRLRCSTSTSPIPFAAPSH-VDHLHHLFPHMDHQLLV 60
           MSAVVCGTKRSFFEELPPSPPI+KRLRCS+STSPI F APS  +DHLHHLFPHMDHQLLV
Sbjct: 1   MSAVVCGTKRSFFEELPPSPPISKRLRCSSSTSPITFPAPSSLIDHLHHLFPHMDHQLLV 60

Query: 61  RALEECGNDLDAAIRSLSDLCLGSAVENPVTTAEPEKNLDQGSFANNGEAAASVNCSAPS 120
           RALEECGNDLDAAI+SLSDLCLGSAVENPV TAEPE NLDQGSFAN+GEAAAS NCS PS
Sbjct: 61  RALEECGNDLDAAIKSLSDLCLGSAVENPVATAEPEPNLDQGSFANDGEAAASENCSVPS 120

Query: 121 SVSLDGRKWIDLFVVEMMNATTIADAKTRAARALEALENSISEHAGVDAAKNFHKENTQL 180
           SVSLDGRKWIDLFVVEM++A  +ADA++RAARALEALENSIS  AGVDAA+NFHKEN QL
Sbjct: 121 SVSLDGRKWIDLFVVEMVSAIDVADARSRAARALEALENSISASAGVDAAQNFHKENMQL 180

Query: 181 KEQIDVLLRENTILKRAVAIQHERQKEFEDKNLELQHLKQMVSQYQEQLRTLEINNYALT 240
           KEQI+VLLRENTILKRAVAIQHERQKEFE KN+ELQHLKQ+V QYQEQLRTLEINNYALT
Sbjct: 181 KEQIEVLLRENTILKRAVAIQHERQKEFEGKNIELQHLKQLVCQYQEQLRTLEINNYALT 240

Query: 241 MHLKQAQQSSSIPGRFHPDVF 261
           MHLKQ +QSSSIPGRFHPDVF
Sbjct: 241 MHLKQTEQSSSIPGRFHPDVF 261

BLAST of CaUC07G136570 vs. NCBI nr
Match: XP_038896372.1 (uncharacterized protein LOC120084606 [Benincasa hispida])

HSP 1 Score: 448.4 bits (1152), Expect = 4.3e-122
Identity = 230/260 (88.46%), Postives = 242/260 (93.08%), Query Frame = 0

Query: 1   MSAVVCGTKRSFFEELPPSPPIAKRLRCSTSTSPIPFAAPSHVDHLHHLFPHMDHQLLVR 60
           MSAVVCGTKRSFFEELPPS PIAKRLRCSTSTSPI FAAPSH+DHLHHLFP MDHQLLV+
Sbjct: 1   MSAVVCGTKRSFFEELPPSTPIAKRLRCSTSTSPIRFAAPSHIDHLHHLFPQMDHQLLVK 60

Query: 61  ALEECGNDLDAAIRSLSDLCLGSAVENPVTTAEPEKNLDQGSFANNGEAAASVNCSAPSS 120
           ALEECGNDLDAAIR+LSD+CLGSAVENPV +AE E NLDQGSF NNGEAAAS N S PSS
Sbjct: 61  ALEECGNDLDAAIRNLSDMCLGSAVENPVASAELETNLDQGSFTNNGEAAASENSSIPSS 120

Query: 121 VSLDGRKWIDLFVVEMMNATTIADAKTRAARALEALENSISEHAGVDAAKNFHKENTQLK 180
           VSLDGRKWIDLFVVEMMNATT+ADAKTRAARALEALENSI+  A VDAA+NFHKEN QLK
Sbjct: 121 VSLDGRKWIDLFVVEMMNATTVADAKTRAARALEALENSITAQARVDAAQNFHKENMQLK 180

Query: 181 EQIDVLLRENTILKRAVAIQHERQKEFEDKNLELQHLKQMVSQYQEQLRTLEINNYALTM 240
           E I+VL+RENTILKRAVAIQHERQKEFEDKNLELQH KQ+V+QYQEQLRTLEINNYALTM
Sbjct: 181 EHIEVLVRENTILKRAVAIQHERQKEFEDKNLELQHFKQLVAQYQEQLRTLEINNYALTM 240

Query: 241 HLKQAQQSSSIPGRFHPDVF 261
           HLK AQQSSSIPGR HPDVF
Sbjct: 241 HLKHAQQSSSIPGRVHPDVF 260

BLAST of CaUC07G136570 vs. NCBI nr
Match: XP_022955806.1 (uncharacterized protein LOC111457686 [Cucurbita moschata])

HSP 1 Score: 448.4 bits (1152), Expect = 4.3e-122
Identity = 230/261 (88.12%), Postives = 243/261 (93.10%), Query Frame = 0

Query: 1   MSAVVCGTKRSFFEELPPSPPIAKRLRCSTSTSPIPFAAPSH-VDHLHHLFPHMDHQLLV 60
           MSAVVCGTKRSFFEELPPSPPI+KRLRCS+STSPI F APS  +DHLHHLFPHMDHQLLV
Sbjct: 1   MSAVVCGTKRSFFEELPPSPPISKRLRCSSSTSPITFPAPSSLIDHLHHLFPHMDHQLLV 60

Query: 61  RALEECGNDLDAAIRSLSDLCLGSAVENPVTTAEPEKNLDQGSFANNGEAAASVNCSAPS 120
           RALEECGNDLDAAI+SLSDLCLGSAVENPV TAEPE NLDQGS ANNGEAAAS NCS PS
Sbjct: 61  RALEECGNDLDAAIKSLSDLCLGSAVENPVATAEPEPNLDQGSIANNGEAAASENCSVPS 120

Query: 121 SVSLDGRKWIDLFVVEMMNATTIADAKTRAARALEALENSISEHAGVDAAKNFHKENTQL 180
           SVSLDGRKWIDLFVVEM +A  +ADA++RAARALEALENSIS  AGVDAA+NFHKEN QL
Sbjct: 121 SVSLDGRKWIDLFVVEMASAVDVADARSRAARALEALENSISARAGVDAAQNFHKENMQL 180

Query: 181 KEQIDVLLRENTILKRAVAIQHERQKEFEDKNLELQHLKQMVSQYQEQLRTLEINNYALT 240
           KEQI+VLLRENTILKRAVAIQHERQKEFE KN+ELQHLKQ+V QYQEQLRTLEINNYALT
Sbjct: 181 KEQIEVLLRENTILKRAVAIQHERQKEFEGKNIELQHLKQLVCQYQEQLRTLEINNYALT 240

Query: 241 MHLKQAQQSSSIPGRFHPDVF 261
           MHLKQ +QSSSIPGRFHPDVF
Sbjct: 241 MHLKQTEQSSSIPGRFHPDVF 261

BLAST of CaUC07G136570 vs. NCBI nr
Match: XP_023528850.1 (uncharacterized protein LOC111791655 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023528851.1 uncharacterized protein LOC111791655 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 448.0 bits (1151), Expect = 5.7e-122
Identity = 230/261 (88.12%), Postives = 243/261 (93.10%), Query Frame = 0

Query: 1   MSAVVCGTKRSFFEELPPSPPIAKRLRCSTSTSPIPFAAPSH-VDHLHHLFPHMDHQLLV 60
           MSAVVCGTKRSFFEELPPSPPI+KRLRCS+STSPI F APS  +DHLHHLFPHMDHQLLV
Sbjct: 1   MSAVVCGTKRSFFEELPPSPPISKRLRCSSSTSPITFPAPSSLIDHLHHLFPHMDHQLLV 60

Query: 61  RALEECGNDLDAAIRSLSDLCLGSAVENPVTTAEPEKNLDQGSFANNGEAAASVNCSAPS 120
           RALEECGNDLDAAI+SLSDLCLGSAVENPV TAEPE NLDQGS ANNGEAAAS NCS PS
Sbjct: 61  RALEECGNDLDAAIKSLSDLCLGSAVENPVATAEPEPNLDQGSIANNGEAAASENCSVPS 120

Query: 121 SVSLDGRKWIDLFVVEMMNATTIADAKTRAARALEALENSISEHAGVDAAKNFHKENTQL 180
           SVSLDGRKWIDLFVVEM +A  +ADA++RAARALEALENSIS  AGVDAA+NFHKEN QL
Sbjct: 121 SVSLDGRKWIDLFVVEMASAIDVADARSRAARALEALENSISARAGVDAAQNFHKENMQL 180

Query: 181 KEQIDVLLRENTILKRAVAIQHERQKEFEDKNLELQHLKQMVSQYQEQLRTLEINNYALT 240
           KEQI+VLLRENTILKRAVAIQHERQKEFE KN+ELQHLKQ+V QYQEQLRTLEINNYALT
Sbjct: 181 KEQIEVLLRENTILKRAVAIQHERQKEFEGKNIELQHLKQLVCQYQEQLRTLEINNYALT 240

Query: 241 MHLKQAQQSSSIPGRFHPDVF 261
           MHLKQ +QSSSIPGRFHPDVF
Sbjct: 241 MHLKQTEQSSSIPGRFHPDVF 261

BLAST of CaUC07G136570 vs. NCBI nr
Match: XP_022955509.1 (uncharacterized protein LOC111457514 [Cucurbita moschata])

HSP 1 Score: 448.0 bits (1151), Expect = 5.7e-122
Identity = 229/261 (87.74%), Postives = 245/261 (93.87%), Query Frame = 0

Query: 1   MSAVVCGTKRSFFEELPPSPPIAKRLRCSTSTSPIPFAAPSH-VDHLHHLFPHMDHQLLV 60
           MSAVVCGTKRSFFEE+PPSPPI+KRLRCS+STSPI F APS  +DHLHHLFPHMDHQLLV
Sbjct: 1   MSAVVCGTKRSFFEEIPPSPPISKRLRCSSSTSPITFPAPSSLIDHLHHLFPHMDHQLLV 60

Query: 61  RALEECGNDLDAAIRSLSDLCLGSAVENPVTTAEPEKNLDQGSFANNGEAAASVNCSAPS 120
           RALEECGNDLDAAI+SLSDLCLGSAVENPV TAEPE NLDQGSFAN+GEAAAS NCS PS
Sbjct: 61  RALEECGNDLDAAIKSLSDLCLGSAVENPVATAEPEPNLDQGSFANDGEAAASENCSVPS 120

Query: 121 SVSLDGRKWIDLFVVEMMNATTIADAKTRAARALEALENSISEHAGVDAAKNFHKENTQL 180
           SVSLDGRKWIDLFVVEM++A  +ADA++RAARALEALENSIS  AGVDAA+NFHKEN QL
Sbjct: 121 SVSLDGRKWIDLFVVEMVSAIDVADARSRAARALEALENSISASAGVDAAQNFHKENMQL 180

Query: 181 KEQIDVLLRENTILKRAVAIQHERQKEFEDKNLELQHLKQMVSQYQEQLRTLEINNYALT 240
           KEQI+VLLRENTILKRAVAIQHERQKEFE KN+ELQHLKQ+V QYQEQLRTLEINNYALT
Sbjct: 181 KEQIEVLLRENTILKRAVAIQHERQKEFEGKNIELQHLKQLVCQYQEQLRTLEINNYALT 240

Query: 241 MHLKQAQQSSSIPGRFHPDVF 261
           MHLKQ +QSSSIPGRFHPDVF
Sbjct: 241 MHLKQTEQSSSIPGRFHPDVF 261

BLAST of CaUC07G136570 vs. ExPASy TrEMBL
Match: A0A6J1GUV7 (uncharacterized protein LOC111457686 OS=Cucurbita moschata OX=3662 GN=LOC111457686 PE=4 SV=1)

HSP 1 Score: 448.4 bits (1152), Expect = 2.1e-122
Identity = 230/261 (88.12%), Postives = 243/261 (93.10%), Query Frame = 0

Query: 1   MSAVVCGTKRSFFEELPPSPPIAKRLRCSTSTSPIPFAAPSH-VDHLHHLFPHMDHQLLV 60
           MSAVVCGTKRSFFEELPPSPPI+KRLRCS+STSPI F APS  +DHLHHLFPHMDHQLLV
Sbjct: 1   MSAVVCGTKRSFFEELPPSPPISKRLRCSSSTSPITFPAPSSLIDHLHHLFPHMDHQLLV 60

Query: 61  RALEECGNDLDAAIRSLSDLCLGSAVENPVTTAEPEKNLDQGSFANNGEAAASVNCSAPS 120
           RALEECGNDLDAAI+SLSDLCLGSAVENPV TAEPE NLDQGS ANNGEAAAS NCS PS
Sbjct: 61  RALEECGNDLDAAIKSLSDLCLGSAVENPVATAEPEPNLDQGSIANNGEAAASENCSVPS 120

Query: 121 SVSLDGRKWIDLFVVEMMNATTIADAKTRAARALEALENSISEHAGVDAAKNFHKENTQL 180
           SVSLDGRKWIDLFVVEM +A  +ADA++RAARALEALENSIS  AGVDAA+NFHKEN QL
Sbjct: 121 SVSLDGRKWIDLFVVEMASAVDVADARSRAARALEALENSISARAGVDAAQNFHKENMQL 180

Query: 181 KEQIDVLLRENTILKRAVAIQHERQKEFEDKNLELQHLKQMVSQYQEQLRTLEINNYALT 240
           KEQI+VLLRENTILKRAVAIQHERQKEFE KN+ELQHLKQ+V QYQEQLRTLEINNYALT
Sbjct: 181 KEQIEVLLRENTILKRAVAIQHERQKEFEGKNIELQHLKQLVCQYQEQLRTLEINNYALT 240

Query: 241 MHLKQAQQSSSIPGRFHPDVF 261
           MHLKQ +QSSSIPGRFHPDVF
Sbjct: 241 MHLKQTEQSSSIPGRFHPDVF 261

BLAST of CaUC07G136570 vs. ExPASy TrEMBL
Match: A0A6J1GWH1 (uncharacterized protein LOC111457514 OS=Cucurbita moschata OX=3662 GN=LOC111457514 PE=4 SV=1)

HSP 1 Score: 448.0 bits (1151), Expect = 2.7e-122
Identity = 229/261 (87.74%), Postives = 245/261 (93.87%), Query Frame = 0

Query: 1   MSAVVCGTKRSFFEELPPSPPIAKRLRCSTSTSPIPFAAPSH-VDHLHHLFPHMDHQLLV 60
           MSAVVCGTKRSFFEE+PPSPPI+KRLRCS+STSPI F APS  +DHLHHLFPHMDHQLLV
Sbjct: 1   MSAVVCGTKRSFFEEIPPSPPISKRLRCSSSTSPITFPAPSSLIDHLHHLFPHMDHQLLV 60

Query: 61  RALEECGNDLDAAIRSLSDLCLGSAVENPVTTAEPEKNLDQGSFANNGEAAASVNCSAPS 120
           RALEECGNDLDAAI+SLSDLCLGSAVENPV TAEPE NLDQGSFAN+GEAAAS NCS PS
Sbjct: 61  RALEECGNDLDAAIKSLSDLCLGSAVENPVATAEPEPNLDQGSFANDGEAAASENCSVPS 120

Query: 121 SVSLDGRKWIDLFVVEMMNATTIADAKTRAARALEALENSISEHAGVDAAKNFHKENTQL 180
           SVSLDGRKWIDLFVVEM++A  +ADA++RAARALEALENSIS  AGVDAA+NFHKEN QL
Sbjct: 121 SVSLDGRKWIDLFVVEMVSAIDVADARSRAARALEALENSISASAGVDAAQNFHKENMQL 180

Query: 181 KEQIDVLLRENTILKRAVAIQHERQKEFEDKNLELQHLKQMVSQYQEQLRTLEINNYALT 240
           KEQI+VLLRENTILKRAVAIQHERQKEFE KN+ELQHLKQ+V QYQEQLRTLEINNYALT
Sbjct: 181 KEQIEVLLRENTILKRAVAIQHERQKEFEGKNIELQHLKQLVCQYQEQLRTLEINNYALT 240

Query: 241 MHLKQAQQSSSIPGRFHPDVF 261
           MHLKQ +QSSSIPGRFHPDVF
Sbjct: 241 MHLKQTEQSSSIPGRFHPDVF 261

BLAST of CaUC07G136570 vs. ExPASy TrEMBL
Match: A0A0A0KVN0 (CUE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G006460 PE=4 SV=1)

HSP 1 Score: 446.0 bits (1146), Expect = 1.0e-121
Identity = 232/260 (89.23%), Postives = 243/260 (93.46%), Query Frame = 0

Query: 1   MSAVVCGTKRSFFEELPPSPPIAKRLRCSTSTSPIPFAAPSHVDHLHHLFPHMDHQLLVR 60
           MSAVVCG+KRSFFEELPPSPPIAKRLRCSTSTSPI FAAPSH+DHL HLFP MD QLLVR
Sbjct: 1   MSAVVCGSKRSFFEELPPSPPIAKRLRCSTSTSPIRFAAPSHIDHLQHLFPQMDRQLLVR 60

Query: 61  ALEECGNDLDAAIRSLSDLCLGSAVENPVTTAEPEKNLDQGSFANNGEAAASVNCSAPSS 120
           ALEECGNDLDAAIRSLSDLCLGSAVENPV +AEPE NLDQGS ANNGE AAS N S  SS
Sbjct: 61  ALEECGNDLDAAIRSLSDLCLGSAVENPVASAEPETNLDQGSIANNGEVAASENSS--SS 120

Query: 121 VSLDGRKWIDLFVVEMMNATTIADAKTRAARALEALENSISEHAGVDAAKNFHKENTQLK 180
           VSLDGRKWIDLFVVEM NATT+ADAKTRAARALEALENSI+  A VDAA+NFHKEN QLK
Sbjct: 121 VSLDGRKWIDLFVVEMTNATTVADAKTRAARALEALENSITARASVDAAQNFHKENMQLK 180

Query: 181 EQIDVLLRENTILKRAVAIQHERQKEFEDKNLELQHLKQMVSQYQEQLRTLEINNYALTM 240
           EQI++L+RENTILKRAVAIQHERQKEFEDKNLELQHLKQ+V+QYQEQLRTLEINNYALTM
Sbjct: 181 EQIELLVRENTILKRAVAIQHERQKEFEDKNLELQHLKQLVAQYQEQLRTLEINNYALTM 240

Query: 241 HLKQAQQSSSIPGRFHPDVF 261
           HLKQAQQSSSIPGRFHPDVF
Sbjct: 241 HLKQAQQSSSIPGRFHPDVF 258

BLAST of CaUC07G136570 vs. ExPASy TrEMBL
Match: A0A6J1IXF9 (uncharacterized protein LOC111480055 OS=Cucurbita maxima OX=3661 GN=LOC111480055 PE=4 SV=1)

HSP 1 Score: 444.5 bits (1142), Expect = 3.0e-121
Identity = 228/261 (87.36%), Postives = 243/261 (93.10%), Query Frame = 0

Query: 1   MSAVVCGTKRSFFEELPPSPPIAKRLRCSTSTSPIPFAAPSH-VDHLHHLFPHMDHQLLV 60
           MSAVVCGTKRSFFEELPPSPPI+KRLRCS+STSPI F APS  +DHLHHLFPHMDHQLLV
Sbjct: 1   MSAVVCGTKRSFFEELPPSPPISKRLRCSSSTSPITFPAPSSLIDHLHHLFPHMDHQLLV 60

Query: 61  RALEECGNDLDAAIRSLSDLCLGSAVENPVTTAEPEKNLDQGSFANNGEAAASVNCSAPS 120
           RALEECGNDLDAAI++LSDLCLGSAVENPV TAEPE NLDQGSFAN+GEAAAS NCS PS
Sbjct: 61  RALEECGNDLDAAIKNLSDLCLGSAVENPVATAEPEPNLDQGSFANDGEAAASENCSVPS 120

Query: 121 SVSLDGRKWIDLFVVEMMNATTIADAKTRAARALEALENSISEHAGVDAAKNFHKENTQL 180
           SVSLDGRKWIDLFVVEM +A  +ADA++RAARALEALENSIS  AGVDAA+NFHKEN QL
Sbjct: 121 SVSLDGRKWIDLFVVEMASAIDVADARSRAARALEALENSISARAGVDAAQNFHKENMQL 180

Query: 181 KEQIDVLLRENTILKRAVAIQHERQKEFEDKNLELQHLKQMVSQYQEQLRTLEINNYALT 240
           KEQI+VLLRENTILKRAVAIQHERQKEFE KN+ LQHLKQ+V QYQEQLRTLEINNYALT
Sbjct: 181 KEQIEVLLRENTILKRAVAIQHERQKEFEGKNIGLQHLKQLVCQYQEQLRTLEINNYALT 240

Query: 241 MHLKQAQQSSSIPGRFHPDVF 261
           MHLKQ +QSSSIPGRFHPDVF
Sbjct: 241 MHLKQTEQSSSIPGRFHPDVF 261

BLAST of CaUC07G136570 vs. ExPASy TrEMBL
Match: A0A6J1DJ22 (uncharacterized protein LOC111020986 OS=Momordica charantia OX=3673 GN=LOC111020986 PE=4 SV=1)

HSP 1 Score: 440.3 bits (1131), Expect = 5.7e-120
Identity = 225/260 (86.54%), Postives = 240/260 (92.31%), Query Frame = 0

Query: 1   MSAVVCGTKRSFFEELPPSPPIAKRLRCSTSTSPIPFAAPSHVDHLHHLFPHMDHQLLVR 60
           MSAVVCGTKRSFFEELPPSPPIAKRLRCSTSTSPI FAAPS +D LH LFPHMDHQLL R
Sbjct: 1   MSAVVCGTKRSFFEELPPSPPIAKRLRCSTSTSPIRFAAPSLIDQLHDLFPHMDHQLLAR 60

Query: 61  ALEECGNDLDAAIRSLSDLCLGSAVENPVTTAEPEKNLDQGSFANNGEAAASVNCSAPSS 120
           ALEECGNDLDAAI+ LSDLCLGSA ENP  TAEPE NLDQGSF N+GEAAAS N SAPSS
Sbjct: 61  ALEECGNDLDAAIKCLSDLCLGSAAENPGATAEPETNLDQGSFVNDGEAAASENLSAPSS 120

Query: 121 VSLDGRKWIDLFVVEMMNATTIADAKTRAARALEALENSISEHAGVDAAKNFHKENTQLK 180
           VSLDGR+W+DLFV EMM+AT++ DA+TRAARALEALENSIS  AG DA +NFHKEN QLK
Sbjct: 121 VSLDGREWVDLFVREMMSATSVDDARTRAARALEALENSISARAGADAVQNFHKENIQLK 180

Query: 181 EQIDVLLRENTILKRAVAIQHERQKEFEDKNLELQHLKQMVSQYQEQLRTLEINNYALTM 240
           EQI+VL+RENTILKRAV+IQHERQKEF+DKNLELQHLKQ+VSQYQEQLRTLEINNYALTM
Sbjct: 181 EQIEVLIRENTILKRAVSIQHERQKEFDDKNLELQHLKQLVSQYQEQLRTLEINNYALTM 240

Query: 241 HLKQAQQSSSIPGRFHPDVF 261
           HLKQAQQSSSIPGRFHPDVF
Sbjct: 241 HLKQAQQSSSIPGRFHPDVF 260

BLAST of CaUC07G136570 vs. TAIR 10
Match: AT5G32440.1 (Ubiquitin system component Cue protein )

HSP 1 Score: 263.1 bits (671), Expect = 2.4e-70
Identity = 148/275 (53.82%), Postives = 195/275 (70.91%), Query Frame = 0

Query: 1   MSAVVCGTKRSFFEEL-PPSPPIAKRLRCSTSTS------PIPFAAPSHVDHLHHLFPHM 60
           MSA+VCG KRS FE+L   SPP++K+LRC +S+S      PIP ++   +DHL  +FP M
Sbjct: 1   MSAIVCG-KRSLFEDLAAASPPVSKKLRCFSSSSSSRFSPPIPPSSSLLLDHLAAIFPDM 60

Query: 61  DHQLLVRALEECGNDLDAAIRSLSDLCLGSAVEN--------PVTTAEPEKNLDQGSFAN 120
           D Q+L RA+EECG+DLD+AIR L+ L L SA +N        PV   EP     Q   A 
Sbjct: 61  DKQILERAIEECGDDLDSAIRCLNQLRLESANKNSDSATNQSPVVIQEPNVEPQQQGSAK 120

Query: 121 NGEAAASVNCSAPSSVSLDGRKWIDLFVVEMMNATTIADAKTRAARALEALENSISEHAG 180
                       P+ ++LDG +W++LFV EMMNA+ + DAK RAARALEALE SI+   G
Sbjct: 121 ----------EEPNVLNLDGTEWVELFVREMMNASDMKDAKARAARALEALEKSINARTG 180

Query: 181 VDAAKNFHKENTQLKEQIDVLLRENTILKRAVAIQHERQKEFEDKNLELQHLKQMVSQYQ 240
            DA +N  +EN  LK+Q++ +++EN++LKRAV  Q +RQ+E ED++ ELQHL+Q+V+QYQ
Sbjct: 181 TDAMQNLQQENMMLKQQLEAIVQENSLLKRAVVTQQKRQRESEDQSQELQHLRQLVTQYQ 240

Query: 241 EQLRTLEINNYALTMHLKQAQQSSSIPGRFHPDVF 261
           EQLRTLE+NNYALT+HLKQAQQ+SSIPGR+HPDVF
Sbjct: 241 EQLRTLEVNNYALTLHLKQAQQNSSIPGRYHPDVF 264

BLAST of CaUC07G136570 vs. TAIR 10
Match: AT5G32440.3 (Ubiquitin system component Cue protein )

HSP 1 Score: 262.7 bits (670), Expect = 3.1e-70
Identity = 149/275 (54.18%), Postives = 197/275 (71.64%), Query Frame = 0

Query: 1   MSAVVCGTKRSFFEEL-PPSPPIAKRLRCSTSTS------PIPFAAPSHVDHLHHLFPHM 60
           MSA+VCG KRS FE+L   SPP++K+LRC +S+S      PIP ++   +DHL  +FP M
Sbjct: 1   MSAIVCG-KRSLFEDLAAASPPVSKKLRCFSSSSSSRFSPPIPPSSSLLLDHLAAIFPDM 60

Query: 61  DHQLLVRALEECGNDLDAAIRSLSDLCLGSAVEN--------PVTTAEPEKNLDQGSFAN 120
           D Q+L RA+EECG+DLD+AIR L+ L L SA +N        PV   EP     Q     
Sbjct: 61  DKQILERAIEECGDDLDSAIRCLNQLRLESANKNSDSATNQSPVVIQEPNVEPQQ----- 120

Query: 121 NGEAAASVNCSAPSSVSLDGRKWIDLFVVEMMNATTIADAKTRAARALEALENSISEHAG 180
            G +A       P+ ++LDG +W++LFV EMMNA+ + DAK RAARALEALE SI+   G
Sbjct: 121 QGRSAK----EEPNVLNLDGTEWVELFVREMMNASDMKDAKARAARALEALEKSINARTG 180

Query: 181 VDAAKNFHKENTQLKEQIDVLLRENTILKRAVAIQHERQKEFEDKNLELQHLKQMVSQYQ 240
            DA +N  +EN  LK+Q++ +++EN++LKRAV  Q +RQ+E ED++ ELQHL+Q+V+QYQ
Sbjct: 181 TDAMQNLQQENMMLKQQLEAIVQENSLLKRAVVTQQKRQRESEDQSQELQHLRQLVTQYQ 240

Query: 241 EQLRTLEINNYALTMHLKQAQQSSSIPGRFHPDVF 261
           EQLRTLE+NNYALT+HLKQAQQ+SSIPGR+HPDVF
Sbjct: 241 EQLRTLEVNNYALTLHLKQAQQNSSIPGRYHPDVF 265

BLAST of CaUC07G136570 vs. TAIR 10
Match: AT1G80040.1 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Ubiquitin system component Cue (InterPro:IPR003892); BEST Arabidopsis thaliana protein match is: Ubiquitin system component Cue protein (TAIR:AT5G32440.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 200.7 bits (509), Expect = 1.5e-51
Identity = 118/262 (45.04%), Postives = 165/262 (62.98%), Query Frame = 0

Query: 1   MSAVVCGTKRSFFEELPPSPPIAKRLRC-STSTSPIPFAAP-SHVDHLHHLFPHMDHQLL 60
           MSAV CGTKRS+F++   SPP +KR RC S S SPI  + P S +D LH  FPH++  +L
Sbjct: 1   MSAVYCGTKRSYFDD-NSSPPSSKRFRCFSPSNSPIWSSPPSSSLDQLHSAFPHIELTVL 60

Query: 61  VRALEECGNDLDAAIRSLSDLCLGSAVENPVTTAEPEKNLDQGSFANNGEAAASVNCSAP 120
           V+ALE+ G+D +AA++SL               +  EK  ++   A  G A    +    
Sbjct: 61  VKALEDNGSDFNAAMKSLYSF-----------ASSEEKKAEE--LAAGGAATQETDAVCG 120

Query: 121 SSVSLDGRKWIDLFVVEMMNATTIADAKTRAARALEALENSISEHAGVDAAKNFHKENTQ 180
            +    G  W++L V E++ ++   DAK RAAR LEALE  +S  A  +A   F +E   
Sbjct: 121 GNPPTSGDDWVELLVREVLQSSGTDDAKVRAARVLEALEKMLSARAREEAGNKFQEEKVA 180

Query: 181 LKEQIDVLLRENTILKRAVAIQHERQKEFEDKNLELQHLKQMVSQYQEQLRTLEINNYAL 240
           +++Q++ L+++NT+LKRAVAIQHERQK  ED N +L  LKQ+V QYQE+LR LE+NNYAL
Sbjct: 181 VQQQVETLVKDNTVLKRAVAIQHERQKALEDANHQLGLLKQLVPQYQEKLRNLEVNNYAL 240

Query: 241 TMHLKQAQQSSSIPGRFHPDVF 261
            M L+Q +  +S+P RF+PDVF
Sbjct: 241 RMQLQQVEHGNSMPARFNPDVF 248

BLAST of CaUC07G136570 vs. TAIR 10
Match: AT1G80040.3 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; BEST Arabidopsis thaliana protein match is: Ubiquitin system component Cue protein (TAIR:AT5G32440.1). )

HSP 1 Score: 194.9 bits (494), Expect = 8.1e-50
Identity = 119/273 (43.59%), Postives = 166/273 (60.81%), Query Frame = 0

Query: 1   MSAVVCGTKRSFFEELPPSPPIAKRLRC-STSTSPIPFAAP-SHVDHLHHLFPHMD---- 60
           MSAV CGTKRS+F++   SPP +KR RC S S SPI  + P S +D LH  FPH++    
Sbjct: 1   MSAVYCGTKRSYFDD-NSSPPSSKRFRCFSPSNSPIWSSPPSSSLDQLHSAFPHIELTVA 60

Query: 61  -------HQLLVRALEECGNDLDAAIRSLSDLCLGSAVENPVTTAEPEKNLDQGSFANNG 120
                   Q+LV+ALE+ G+D +AA++SL               +  EK  ++   A  G
Sbjct: 61  SKIHVSVAQVLVKALEDNGSDFNAAMKSLYSF-----------ASSEEKKAEE--LAAGG 120

Query: 121 EAAASVNCSAPSSVSLDGRKWIDLFVVEMMNATTIADAKTRAARALEALENSISEHAGVD 180
            A    +     +    G  W++L V E++ ++   DAK RAAR LEALE  +S  A  +
Sbjct: 121 AATQETDAVCGGNPPTSGDDWVELLVREVLQSSGTDDAKVRAARVLEALEKMLSARAREE 180

Query: 181 AAKNFHKENTQLKEQIDVLLRENTILKRAVAIQHERQKEFEDKNLELQHLKQMVSQYQEQ 240
           A   F +E   +++Q++ L+++NT+LKRAVAIQHERQK  ED N +L  LKQ+V QYQE+
Sbjct: 181 AGNKFQEEKVAVQQQVETLVKDNTVLKRAVAIQHERQKALEDANHQLGLLKQLVPQYQEK 240

Query: 241 LRTLEINNYALTMHLKQAQQSSSIPGRFHPDVF 261
           LR LE+NNYAL M L+Q +  +S+P RF+PDVF
Sbjct: 241 LRNLEVNNYALRMQLQQVEHGNSMPARFNPDVF 259

BLAST of CaUC07G136570 vs. TAIR 10
Match: AT5G32440.2 (Ubiquitin system component Cue protein )

HSP 1 Score: 142.5 bits (358), Expect = 4.7e-34
Identity = 89/190 (46.84%), Postives = 118/190 (62.11%), Query Frame = 0

Query: 1   MSAVVCGTKRSFFEEL-PPSPPIAKRLRCSTSTS------PIPFAAPSHVDHLHHLFPHM 60
           MSA+VCG KRS FE+L   SPP++K+LRC +S+S      PIP ++   +DHL  +FP M
Sbjct: 1   MSAIVCG-KRSLFEDLAAASPPVSKKLRCFSSSSSSRFSPPIPPSSSLLLDHLAAIFPDM 60

Query: 61  DHQLLVRALEECGNDLDAAIRSLSDLCLGSAVEN--------PVTTAEPEKNLDQGSFAN 120
           D Q+L RA+EECG+DLD+AIR L+ L L SA +N        PV   EP     Q   A 
Sbjct: 61  DKQILERAIEECGDDLDSAIRCLNQLRLESANKNSDSATNQSPVVIQEPNVEPQQQGSAK 120

Query: 121 NGEAAASVNCSAPSSVSLDGRKWIDLFVVEMMNATTIADAKTRAARALEALENSISEHAG 176
                       P+ ++LDG +W++LFV EMMNA+ + DAK RAARALEALE SI+   G
Sbjct: 121 ----------EEPNVLNLDGTEWVELFVREMMNASDMKDAKARAARALEALEKSINARTG 179

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023528958.13.3e-12288.12uncharacterized protein LOC111791720 [Cucurbita pepo subsp. pepo][more]
XP_038896372.14.3e-12288.46uncharacterized protein LOC120084606 [Benincasa hispida][more]
XP_022955806.14.3e-12288.12uncharacterized protein LOC111457686 [Cucurbita moschata][more]
XP_023528850.15.7e-12288.12uncharacterized protein LOC111791655 isoform X1 [Cucurbita pepo subsp. pepo] >XP... [more]
XP_022955509.15.7e-12287.74uncharacterized protein LOC111457514 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1GUV72.1e-12288.12uncharacterized protein LOC111457686 OS=Cucurbita moschata OX=3662 GN=LOC1114576... [more]
A0A6J1GWH12.7e-12287.74uncharacterized protein LOC111457514 OS=Cucurbita moschata OX=3662 GN=LOC1114575... [more]
A0A0A0KVN01.0e-12189.23CUE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G006460 PE=4 SV... [more]
A0A6J1IXF93.0e-12187.36uncharacterized protein LOC111480055 OS=Cucurbita maxima OX=3661 GN=LOC111480055... [more]
A0A6J1DJ225.7e-12086.54uncharacterized protein LOC111020986 OS=Momordica charantia OX=3673 GN=LOC111020... [more]
Match NameE-valueIdentityDescription
AT5G32440.12.4e-7053.82Ubiquitin system component Cue protein [more]
AT5G32440.33.1e-7054.18Ubiquitin system component Cue protein [more]
AT1G80040.11.5e-5145.04FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknow... [more]
AT1G80040.38.1e-5043.59FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknow... [more]
AT5G32440.24.7e-3446.84Ubiquitin system component Cue protein [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (USVL246-FR2) v1
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 207..234
NoneNo IPR availableCOILSCoilCoilcoord: 169..196
NoneNo IPR availableCOILSCoilCoilcoord: 142..162
NoneNo IPR availableGENE3D1.10.8.10coord: 37..81
e-value: 4.9E-6
score: 28.0
NoneNo IPR availablePANTHERPTHR31245:SF20F18B13.13 PROTEINcoord: 1..260
NoneNo IPR availablePANTHERPTHR31245UBIQUITIN SYSTEM COMPONENT CUE PROTEINcoord: 1..260
NoneNo IPR availableCDDcd14279CUEcoord: 43..76
e-value: 3.66887E-8
score: 46.3073
IPR003892Ubiquitin system component CUEPFAMPF02845CUEcoord: 40..77
e-value: 2.5E-7
score: 30.2
IPR003892Ubiquitin system component CUEPROSITEPS51140CUEcoord: 38..84
score: 10.917902
IPR009060UBA-like superfamilySUPERFAMILY46934UBA-likecoord: 41..80

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CaUC07G136570.1CaUC07G136570.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0043130 ubiquitin binding
molecular_function GO:0005515 protein binding