CaUC07G125780 (gene) Watermelon (USVL246-FR2) v1

Overview


Name	CaUC07G125780
Type	gene
Organism	Citrullus amarus (Watermelon (USVL246-FR2) v1)
Description	Trypsin inhibitor
Location	Ciama_Chr07: 813911 .. 814248 (+)
RNA-Seq Expression	CaUC07G125780
Synteny	CaUC07G125780

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTGAAGAAAAAATTGTTCTTCCTTCAGGTTAGTAAGACTTCTCCTTCCCACTTGGGTGTTTTCAAATGATTCTTTTGTCCTTTTATTTTTTCTTAATATTTTGAATAACTATTTACTTTTTTACCATTCAATTTACCTAGGGAAATCTTCGTGGCCCGAACTCGTATTCATCGAATCTTCCACTGCAATATGTCTTATAGAGAGAGATAATCCTAATGTGAAGGCAGTTGTGCTATTAGCTGGATCTCCAGTCACTAAAGATTTAAGGCTAGATCGAGTTCGAGTTTTTGTTAACATAAATGGTGTGGTGGTTAGCATTCCTACGGTTGGTTGA

mRNA sequence

ATGGCTGAAGAAAAAATTGTTCTTCCTTCAGGGAAATCTTCGTGGCCCGAACTCGTATTCATCGAATCTTCCACTGCAATATGTCTTATAGAGAGAGATAATCCTAATGTGAAGGCAGTTGTGCTATTAGCTGGATCTCCAGTCACTAAAGATTTAAGGCTAGATCGAGTTCGAGTTTTTGTTAACATAAATGGTGTGGTGGTTAGCATTCCTACGGTTGGTTGA

Coding sequence (CDS)

ATGGCTGAAGAAAAAATTGTTCTTCCTTCAGGGAAATCTTCGTGGCCCGAACTCGTATTCATCGAATCTTCCACTGCAATATGTCTTATAGAGAGAGATAATCCTAATGTGAAGGCAGTTGTGCTATTAGCTGGATCTCCAGTCACTAAAGATTTAAGGCTAGATCGAGTTCGAGTTTTTGTTAACATAAATGGTGTGGTGGTTAGCATTCCTACGGTTGGTTGA

Protein sequence

MAEEKIVLPSGKSSWPELVFIESSTAICLIERDNPNVKAVVLLAGSPVTKDLRLDRVRVFVNINGVVVSIPTVG

Homology

[Close]

BLAST of CaUC07G125780 vs. NCBI nr
Match: XP_011654473.1 (inhibitor of trypsin and hageman factor [Cucumis sativus] >KGN49621.1 hypothetical protein Csa_018430 [Cucumis sativus])

HSP 1 Score: 103.2 bits (256), Expect = 9.7e-19
Identity = 53/75 (70.67%), Postives = 62/75 (82.67%), Query Frame = 0


Query: 1  MAEEKIVLP-SGKSSWPELVFIESSTAICLIERDNPNVKAVVLLAGSPVTKDLRLDRVRV 60
          MAEEKIV P SGKSSWPELVF++SS A+  IERD P+VK VVLLAGSPVT+DLR +RVR+
Sbjct: 1  MAEEKIVFPSSGKSSWPELVFVKSSVAVHWIERDRPDVKPVVLLAGSPVTEDLRPNRVRI 60

Query: 61 FVNINGVVVSIPTVG 75
          FVN+N  VV +P  G
Sbjct: 61 FVNMNDRVVEVPRTG 75

[Close]

BLAST of CaUC07G125780 vs. NCBI nr
Match: KAA0049292.1 (trypsin inhibitor [Cucumis melo var. makuwa] >TYK17266.1 trypsin inhibitor [Cucumis melo var. makuwa])

HSP 1 Score: 83.6 bits (205), Expect = 8.0e-13
Identity = 40/64 (62.50%), Postives = 50/64 (78.12%), Query Frame = 0


Query: 11 GKSSWPELVFIESSTAICLIERDNPNVKAVVLLAGSPVTKDLRLDRVRVFVNINGVVVSI 70
          GK SWP+LV +  S A  +IER+NPNV+AVVL  G+PVTKD R +RVRV+VN NG+V S 
Sbjct: 6  GKRSWPQLVGVGGSVAKAIIERENPNVRAVVLEVGTPVTKDFRCNRVRVWVNKNGLVASP 65

Query: 71 PTVG 75
          PT+G
Sbjct: 66 PTIG 69

[Close]

BLAST of CaUC07G125780 vs. NCBI nr
Match: KAA8527727.1 (hypothetical protein F0562_035404 [Nyssa sinensis])

HSP 1 Score: 83.6 bits (205), Expect = 8.0e-13
Identity = 38/64 (59.38%), Postives = 48/64 (75.00%), Query Frame = 0


Query: 11 GKSSWPELVFIESSTAICLIERDNPNVKAVVLLAGSPVTKDLRLDRVRVFVNINGVVVSI 70
          GKSSWPELV ++  TA   IER+NP V A+++L GSPVT D R DRVRV+VN NG+V  +
Sbjct: 7  GKSSWPELVGVQGETATATIERENPLVTALIVLEGSPVTPDFRCDRVRVYVNENGIVTLV 66

Query: 71 PTVG 75
          P +G
Sbjct: 67 PAIG 70

[Close]

BLAST of CaUC07G125780 vs. NCBI nr
Match: XP_022975284.1 (inhibitor of trypsin and hageman factor [Cucurbita maxima])

HSP 1 Score: 81.6 bits (200), Expect = 3.0e-12
Identity = 39/64 (60.94%), Postives = 49/64 (76.56%), Query Frame = 0


Query: 11 GKSSWPELVFIESSTAICLIERDNPNVKAVVLLAGSPVTKDLRLDRVRVFVNINGVVVSI 70
          GKSSWP LV +  S A  +IER+NPNVKAV+L  G+PVTKD R +RVR++VN  G+VVS 
Sbjct: 6  GKSSWPHLVGVGGSVAKAIIERENPNVKAVILEEGTPVTKDFRCNRVRIWVNKRGLVVSP 65

Query: 71 PTVG 75
          P +G
Sbjct: 66 PRIG 69

[Close]

BLAST of CaUC07G125780 vs. NCBI nr
Match: KAG6571494.1 (hypothetical protein SDJN03_28222, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 81.3 bits (199), Expect = 4.0e-12
Identity = 40/67 (59.70%), Postives = 52/67 (77.61%), Query Frame = 0


Query: 9  PSGKSSWPELVFIESSTAICLIERDNPNVKAV-VLLAGSPVTKDLRLDRVRVFVNINGVV 68
          P GK+ WPELV I+S+ A  +I +DN NV+ V  +LAGS VT+DLR DRVR+FVNI G++
Sbjct: 6  PPGKNRWPELVGIKSTIAEHIIRKDNSNVEHVETILAGSAVTEDLRCDRVRIFVNIKGII 65

Query: 69 VSIPTVG 75
          V IPT+G
Sbjct: 66 VQIPTIG 72

[Close]

BLAST of CaUC07G125780 vs. ExPASy Swiss-Prot
Match: P19873 (Inhibitor of trypsin and hageman factor OS=Cucurbita maxima OX=3661 PE=1 SV=1)

HSP 1 Score: 80.9 bits (198), Expect = 6.8e-15
Identity = 39/64 (60.94%), Postives = 48/64 (75.00%), Query Frame = 0


Query: 11 GKSSWPELVFIESSTAICLIERDNPNVKAVVLLAGSPVTKDLRLDRVRVFVNINGVVVSI 70
          GKSSWP LV +  S A  +IER NPNVKAV+L  G+PVTKD R +RVR++VN  G+VVS 
Sbjct: 5  GKSSWPHLVGVGGSVAKAIIERQNPNVKAVILEEGTPVTKDFRCNRVRIWVNKRGLVVSP 64

Query: 71 PTVG 75
          P +G
Sbjct: 65 PRIG 68

[Close]

BLAST of CaUC07G125780 vs. ExPASy Swiss-Prot
Match: P24076 (Glu S.griseus protease inhibitor OS=Momordica charantia OX=3673 PE=1 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 1.2e-11
Identity = 33/64 (51.56%), Postives = 43/64 (67.19%), Query Frame = 0


Query: 11 GKSSWPELVFIESSTAICLIERDNPNVKAVVLLAGSPVTKDLRLDRVRVFVNINGVVVSI 70
          GK SWP+LV    + A  +IER+NP V+AV++  GSPVT D R DRVRV+V   G+V   
Sbjct: 5  GKRSWPQLVGSTGAAAKAVIERENPRVRAVIVRVGSPVTADFRCDRVRVWVTERGIVARP 64

Query: 71 PTVG 75
          P +G
Sbjct: 65 PAIG 68

[Close]

BLAST of CaUC07G125780 vs. ExPASy Swiss-Prot
Match: P16231 (Wound-induced proteinase inhibitor 1 OS=Solanum peruvianum OX=4082 PE=3 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 4.6e-11
Identity = 35/64 (54.69%), Postives = 46/64 (71.88%), Query Frame = 0


Query: 11  GKSSWPELVFIESSTAICLIERDNPNVKAV-VLLAGSPVTKDLRLDRVRVFVNINGVVVS 70
           GK  WPEL+ + +  A  +IE++NP++  + +LL GSPVTKD R DRVR+FVNI G VV 
Sbjct: 47  GKQFWPELIGVPALYAKGIIEKENPSITNIPILLNGSPVTKDFRCDRVRLFVNILGDVVQ 106

Query: 71  IPTV 74
           IP V
Sbjct: 107 IPRV 110

[Close]

BLAST of CaUC07G125780 vs. ExPASy Swiss-Prot
Match: P82381 (Proteinase inhibitor OS=Linum usitatissimum OX=4006 PE=1 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 1.0e-10
Identity = 33/63 (52.38%), Postives = 43/63 (68.25%), Query Frame = 0


Query: 11 GKSSWPELVFIESSTAICLIERDNPNVKAVVLLAGSPVTKDLRLDRVRVFVNINGVVVSI 70
          GK++WPELV    + A   +ER+N NV A+VL  GS +TKD R DRV V VN +GVV S+
Sbjct: 6  GKNAWPELVGKSGNMAAATVERENRNVHAIVLKEGSAMTKDFRCDRVWVIVNDHGVVTSV 65

Query: 71 PTV 74
          P +
Sbjct: 66 PHI 68

[Close]

BLAST of CaUC07G125780 vs. ExPASy Swiss-Prot
Match: Q6XNP7 (Protease inhibitor HPI OS=Hevea brasiliensis OX=3981 GN=PI1 PE=1 SV=2)

HSP 1 Score: 66.6 bits (161), Expect = 1.3e-10
Identity = 31/63 (49.21%), Postives = 43/63 (68.25%), Query Frame = 0


Query: 12 KSSWPELVFIESSTAICLIERDNPNVKAVVLLAGSPVTKDLRLDRVRVFVNINGVVVSIP 71
          K+SWPELV      A  +I+ +N NVKA+V+  G P+T+DL  +RVRVFV+ N VV  +P
Sbjct: 8  KNSWPELVGTNGDIAAGIIQTENANVKAIVVKEGLPITQDLNFNRVRVFVDENRVVTQVP 67

Query: 72 TVG 75
           +G
Sbjct: 68 AIG 70

[Close]

BLAST of CaUC07G125780 vs. ExPASy TrEMBL
Match: A0A0A0KPI0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G027950 PE=3 SV=1)

HSP 1 Score: 103.2 bits (256), Expect = 4.7e-19
Identity = 53/75 (70.67%), Postives = 62/75 (82.67%), Query Frame = 0


Query: 1  MAEEKIVLP-SGKSSWPELVFIESSTAICLIERDNPNVKAVVLLAGSPVTKDLRLDRVRV 60
          MAEEKIV P SGKSSWPELVF++SS A+  IERD P+VK VVLLAGSPVT+DLR +RVR+
Sbjct: 1  MAEEKIVFPSSGKSSWPELVFVKSSVAVHWIERDRPDVKPVVLLAGSPVTEDLRPNRVRI 60

Query: 61 FVNINGVVVSIPTVG 75
          FVN+N  VV +P  G
Sbjct: 61 FVNMNDRVVEVPRTG 75

[Close]

BLAST of CaUC07G125780 vs. ExPASy TrEMBL
Match: A0A5A7U0I2 (Trypsin inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G001190 PE=3 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 3.9e-13
Identity = 40/64 (62.50%), Postives = 50/64 (78.12%), Query Frame = 0


Query: 11 GKSSWPELVFIESSTAICLIERDNPNVKAVVLLAGSPVTKDLRLDRVRVFVNINGVVVSI 70
          GK SWP+LV +  S A  +IER+NPNV+AVVL  G+PVTKD R +RVRV+VN NG+V S 
Sbjct: 6  GKRSWPQLVGVGGSVAKAIIERENPNVRAVVLEVGTPVTKDFRCNRVRVWVNKNGLVASP 65

Query: 71 PTVG 75
          PT+G
Sbjct: 66 PTIG 69

[Close]

BLAST of CaUC07G125780 vs. ExPASy TrEMBL
Match: A0A5J5AE65 (Uncharacterized protein OS=Nyssa sinensis OX=561372 GN=F0562_035404 PE=3 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 3.9e-13
Identity = 38/64 (59.38%), Postives = 48/64 (75.00%), Query Frame = 0


Query: 11 GKSSWPELVFIESSTAICLIERDNPNVKAVVLLAGSPVTKDLRLDRVRVFVNINGVVVSI 70
          GKSSWPELV ++  TA   IER+NP V A+++L GSPVT D R DRVRV+VN NG+V  +
Sbjct: 7  GKSSWPELVGVQGETATATIERENPLVTALIVLEGSPVTPDFRCDRVRVYVNENGIVTLV 66

Query: 71 PTVG 75
          P +G
Sbjct: 67 PAIG 70

[Close]

BLAST of CaUC07G125780 vs. ExPASy TrEMBL
Match: A0A6J1IK14 (inhibitor of trypsin and hageman factor OS=Cucurbita maxima OX=3661 GN=LOC111474422 PE=3 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 1.5e-12
Identity = 39/64 (60.94%), Postives = 49/64 (76.56%), Query Frame = 0


Query: 11 GKSSWPELVFIESSTAICLIERDNPNVKAVVLLAGSPVTKDLRLDRVRVFVNINGVVVSI 70
          GKSSWP LV +  S A  +IER+NPNVKAV+L  G+PVTKD R +RVR++VN  G+VVS 
Sbjct: 6  GKSSWPHLVGVGGSVAKAIIERENPNVKAVILEEGTPVTKDFRCNRVRIWVNKRGLVVSP 65

Query: 71 PTVG 75
          P +G
Sbjct: 66 PRIG 69

[Close]

BLAST of CaUC07G125780 vs. ExPASy TrEMBL
Match: A0A5C7ISX9 (Uncharacterized protein OS=Acer yangbiense OX=1000413 GN=EZV62_000797 PE=3 SV=1)

HSP 1 Score: 80.9 bits (198), Expect = 2.5e-12
Identity = 35/64 (54.69%), Postives = 48/64 (75.00%), Query Frame = 0


Query: 11 GKSSWPELVFIESSTAICLIERDNPNVKAVVLLAGSPVTKDLRLDRVRVFVNINGVVVSI 70
          GK+SWPELV  +   A   IER+NPNV A++LL G+PVT+D+   RVRV+VN NG+++ I
Sbjct: 10 GKASWPELVGAQGVDAAATIERENPNVSAIILLEGTPVTRDISCTRVRVWVNTNGIIIEI 69

Query: 71 PTVG 75
          P +G
Sbjct: 70 PRIG 73

[Close]

BLAST of CaUC07G125780 vs. TAIR 10
Match: AT2G38870.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 69.3 bits (168), Expect = 1.5e-12
Identity = 35/63 (55.56%), Postives = 41/63 (65.08%), Query Frame = 0


Query: 12 KSSWPELVFIESSTAICLIERDNPNVKAVVLLAGSPVTKDLRLDRVRVFVNINGVVVSIP 71
          K+SWPEL       A  +IER+NP V A V+L GSPVT D R DRVRVFV+ N +VV  P
Sbjct: 8  KNSWPELTGTNGDYAAVVIERENPTVNAAVILDGSPVTADFRCDRVRVFVDGNRIVVKTP 67

Query: 72 TVG 75
            G
Sbjct: 68 KSG 70

[Close]

BLAST of CaUC07G125780 vs. TAIR 10
Match: AT5G43580.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 60.5 bits (145), Expect = 6.7e-10
Identity = 31/64 (48.44%), Postives = 43/64 (67.19%), Query Frame = 0


Query: 11 GKSSWPELVFIESSTAICLIERDNPNVKAVVLLAGSPVTKDLRLDRVRVFVNINGVVVSI 70
          GKSSWPEL+  +   A  +IER+NP +KAV++L G+ V +     RV V+VN  G+VV I
Sbjct: 36 GKSSWPELLGAKGEDAKEVIERENPKMKAVIILDGTVVPEIFICSRVYVWVNDCGIVVQI 95

Query: 71 PTVG 75
          P +G
Sbjct: 96 PIIG 99

[Close]

BLAST of CaUC07G125780 vs. TAIR 10
Match: AT3G46860.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 47.8 bits (112), Expect = 4.5e-06
Identity = 26/63 (41.27%), Postives = 34/63 (53.97%), Query Frame = 0


Query: 12 KSSWPELVFIESSTAICLIERDNPNVKAVVLLAGSPVTKDLRLDRVRVFVNINGVVVSIP 71
          K  WPELV      A   IER+NPNV A+VL  G    ++   +RV V++  NG V   P
Sbjct: 23 KYRWPELVGKNGQLAKMTIERENPNVLAIVLRYGEKRIENFCCNRVFVYLGSNGQVADAP 82

Query: 72 TVG 75
           +G
Sbjct: 83 MIG 85

The following BLAST results are available for this feature:

BLAST of CaUC07G125780 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011654473.1	9.7e-19	70.67	inhibitor of trypsin and hageman factor [Cucumis sativus] >KGN49621.1 hypothetic...	[more]
KAA0049292.1	8.0e-13	62.50	trypsin inhibitor [Cucumis melo var. makuwa] >TYK17266.1 trypsin inhibitor [Cucu...	[more]
KAA8527727.1	8.0e-13	59.38	hypothetical protein F0562_035404 [Nyssa sinensis]	[more]
XP_022975284.1	3.0e-12	60.94	inhibitor of trypsin and hageman factor [Cucurbita maxima]	[more]
KAG6571494.1	4.0e-12	59.70	hypothetical protein SDJN03_28222, partial [Cucurbita argyrosperma subsp. sorori...	[more]

BLAST of CaUC07G125780 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P19873	6.8e-15	60.94	Inhibitor of trypsin and hageman factor OS=Cucurbita maxima OX=3661 PE=1 SV=1	[more]
P24076	1.2e-11	51.56	Glu S.griseus protease inhibitor OS=Momordica charantia OX=3673 PE=1 SV=1	[more]
P16231	4.6e-11	54.69	Wound-induced proteinase inhibitor 1 OS=Solanum peruvianum OX=4082 PE=3 SV=1	[more]
P82381	1.0e-10	52.38	Proteinase inhibitor OS=Linum usitatissimum OX=4006 PE=1 SV=1	[more]
Q6XNP7	1.3e-10	49.21	Protease inhibitor HPI OS=Hevea brasiliensis OX=3981 GN=PI1 PE=1 SV=2	[more]

BLAST of CaUC07G125780 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KPI0	4.7e-19	70.67	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G027950 PE=3 SV=1	[more]
A0A5A7U0I2	3.9e-13	62.50	Trypsin inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G00...	[more]
A0A5J5AE65	3.9e-13	59.38	Uncharacterized protein OS=Nyssa sinensis OX=561372 GN=F0562_035404 PE=3 SV=1	[more]
A0A6J1IK14	1.5e-12	60.94	inhibitor of trypsin and hageman factor OS=Cucurbita maxima OX=3661 GN=LOC111474...	[more]
A0A5C7ISX9	2.5e-12	54.69	Uncharacterized protein OS=Acer yangbiense OX=1000413 GN=EZV62_000797 PE=3 SV=1	[more]

BLAST of CaUC07G125780 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G38870.1	1.5e-12	55.56	Serine protease inhibitor, potato inhibitor I-type family protein	[more]
AT5G43580.1	6.7e-10	48.44	Serine protease inhibitor, potato inhibitor I-type family protein	[more]
AT3G46860.1	4.5e-06	41.27	Serine protease inhibitor, potato inhibitor I-type family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (USVL246-FR2) v1
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PRINTS	PR00292	POTATOINHBTR	coord: 51..61 score: 59.29 coord: 39..50 score: 46.74 coord: 14..27 score: 39.75
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PFAM	PF00280	potato_inhibit	coord: 12..74 e-value: 1.2E-21 score: 77.2
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PANTHER	PTHR33091	PROTEIN, PUTATIVE, EXPRESSED-RELATED	coord: 6..74
None	No IPR available	GENE3D	3.30.10.10	Trypsin Inhibitor V, subunit A	coord: 8..73 e-value: 9.3E-24 score: 85.5
None	No IPR available	PANTHER	PTHR33091:SF83	ETHYLENE-RESPONSIVE PROTEINASE INHIBITOR 1	coord: 6..74
IPR036354	Proteinase inhibitor I13, potato inhibitor I superfamily	SUPERFAMILY	54654	CI-2 family of serine protease inhibitors	coord: 9..74

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CaUC07G125780.1	CaUC07G125780.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010466	negative regulation of peptidase activity
biological_process	GO:0009611	response to wounding
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity