CaUC06G121850 (gene) Watermelon (USVL246-FR2) v1

Overview


Name	CaUC06G121850
Type	gene
Organism	Citrullus amarus (Watermelon (USVL246-FR2) v1)
Description	protein MIS12 homolog
Location	Ciama_Chr06: 26938110 .. 26941345 (+)
RNA-Seq Expression	CaUC06G121850
Synteny	CaUC06G121850

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTTATTAGCATTTAGCGCGACTATGTAAGCCCGCTCCGTGTCCCCATCATTCTTCGTCACTACCATTTTGGCGGCAAAATTTCATCTTGGGAGCTTCCATTCATCTCTCCATTTGAATTTCCTCTTCATTTTCTTCATTGGGGAACAAGCTTTTTTCAGGAGGCGAAAGGGTAATTTTGATGGAAGGAAGTAAAGGCGAAGCAGTATTCGATTCACTGAATCTGAATCCGCAGCTCTTCATTAATGAAGCTCTCAATATTGTCGACGATTTGCTTGATGACGCTTTCGATTTCTATCAATCGTATTGTTCTTTCTGTTTCCATCTCTTCAACATAGGTTTTCAATTTTGTTCGGTTAATTTTATTGATTCTTGTCAATTCCTTTTCCCTTCAGACAAGCATCGGCCGCCTTGAAGACCGAGGGCTCCGACAGATCCCAGGATCTTACCCTGGTAAGGTTTAATTTGTATTATATTACTTGGCATATTCTTATCAGCCGTCAATTTCTGATTTCTTAAGTTTGTGCTCTTAAGCACTTCTTTGAATTGTTCCGATTTTCTTTGAACAAAATTGTTTTCTTGCCTCGTTTCTGTAGGGAATATCGCGAGTCCGAGCGTTGGTCCAATTGGGTCTGGATAAGCGGTTGGCCATGTGGGAGAAGTACTGTCTCAATCACTGTTTTTCAGTTCCTGAAGGTTTTTCTTTACCCTCAAATGTAGGTTTTTCTTCTACTCTTTCTGTGATAAGGCTCCGGTGCCCAAGGATAAAAATGTCGAAGTCCGCATCAGTATCGAAATTACAAGTTATAGTTTATCAATTGAATATCAATATCGACAGATATTTGTGTAAATCAACAAGTTTATCCAAAAAAAAAAAATTAAATTAATAATTAAGTTATTTTTGCTTTCAAAGAAGTTGTAGTTCTTATTGAGATTAATAGATGACAGCTTCTTATTTTTTTATATATTTATAAAAGATACCGAGATGTCTATAAACCCTTAATATCAATGTCAAATCTTCCTATTTATAGATATATCAACATATCAACAAATAATTGAAACCTTGTCTGTGCTTTGTTACGTGAAGTTGCTCTATTAGTGTTGTTTTTATAACTGAATTAGTGGCCCTCTATGTCCGAATCATTTCACAAGCGATTAATAATTGAGTATGTAAAATGGCTGCTTGTTGATTTTGCGAGTTTCCCACTTCTTGTGTACTGATTAGAGAGCTTCCTCAAATTTTGGTTCTGGGGCGAAATTTTTTTTGGAACTTTGGAGAAATGTGCATCATCAAGTTTCAAGTTTTTTACAAGATATACTCATTGTTTAGATCCAGTTTCGAGTTTCCATATTTTCTGACCTTAGAGCTTCCACATTTTGTTTGCTATAAATCAATGTGGTTCCATTGTCACTAGAAATATGGTCATGATGGTAAAATATTGTTTTTAGCTCTAAAAGGAATGTAAGCAAATGTCTGCAGAATTTTTTAGAAAATCTGTCGTTGGTGCATCATGGCTTTTCTTTTTCAATCATTTTTTGAAGTCTAAACTATGTGAATTCCAGTTCGACTCCTTTCATTTTGAACCAATATAGTCACTTGGGGTTGGGCAATCTCTGTCTGTTTCACTGGCATCTCAATGGAATCTGCGATATCTTATTGTCAAGTTGCTAAATTATCAATGGTTCCCATATTCTGTTTGTGAACTTTGAGTTATGGTTAATTGAGTCTATGTACTCTCTTACTACTTTTTCTTTTTTCTAATGCAATAAACATATTTCTGATTCAGATCTAAATATTTTCTTTAAGTTGTCTTAGGATGAATCACCTGGTGTCACTTCAATAAGTCATGACTATGACGTGGATCTGGATACAGAGTTAGATTTTTTGAGGAATAAGCTCTCTGAGGTATTGAGTGTGTCGTATGTTAATTACTTTTTGTGTTTATGTATATCATATTTCATGAGGAAATGTATTTTTCAGGCCAGAAAAGAGAATATTGTATTGAGCCAAGAGCTACAAGCTTTGGAAAGGCAAACTGCTTCCAGTAACTCCCAAATTAATCATTTCAACGAAGCATTACAACTATATGAGCAAAATTCTGTGAATGAGATGTTCCAGGGTAATTAAATTGTGCTTAATTAAAATCATTTTTCTCTTCCTGCACTCCTGGGTTTACTTGCGCCCTACTCTACTAGTATACTCATGAAGGCATACTCGAAATGAAATATACAAATAATGTATGAAGCTGTTTTTTCTTATAATGAACCTTTATTGTCATAGTCAGCTTGGTAGAGCAAAGTTTCTGTAAACACTAACCTTCTCTCTGACGGATCAATCTGGCCTGCATATCTCATGGTGACACAAATTCTAATAGTTCCAAATGATACTTCTTATTGAACACCTGCAAAATCATTTCATTTAGGCAATTTTTAGATTACCCTAAAGTAAACTCTAGTAGTCCCATTTTCTGTTGTCCATTCCATAAAAGAGGAACTTCAGTATGTGAATAAGGCAATTTATTTTCCTATTTTCATTTTGAAGGCCACTTGGACAAAATTTATTCACTAGTATTAAATTTCATTGTACTGAAATTTTAACGGTTGGCTGGTCATTTGGGGTTTGTGCTATTTTGTGGGATATTTGGGAGAAGACAAATAACAAAGTGTTTAGAGGTGTGGCTGAACCATGGTGATGTTTGGTCCTTGGTGAGGTTTCATGTTTCTCTTTAAAATTCAGTTTCAAAAACCTTTTGTAACCATCCTCTAGATAGTGTCTTATTTAGTTGGAAACCCTTCCTATAGAGGGGTTTTGCAGGCTTGTCCTTTTTGTTGTCTTGTATTCTTCATTTTTTATCTATAAAAACCGTTTTCCCTATCAAACTTCAATGGTTGGCAAGTTAAAGCTGTTCATGATTTTAGGAAGCAGACTTCTTATGCATATCAACTTCTTGCAGAAATGATGGGAACTGCATCGGAGCTACGAGCGAAAATAGGAAAGCTGAAGAAAAGGAGGATGGAGGAAGCCAAGCTCAGTAAAGTAGAGAAGGTTCATACAAACGGAGACATCTCTCACCATCACAATGGTAGTAGGATTTTCAAGCTACTTTATTATCATTTTAGCTTTCTGGAAGTTGAATTTCATCTCACATGCTGGTTGATACTTGTTTCTGTTCTGTAGGTTTCTCTAATGCTAAGCTGGATGACATTCAAGAATTCTTAGCTGATTTGAAGACCCTT

mRNA sequence

ATTTATTAGCATTTAGCGCGACTATGTAAGCCCGCTCCGTGTCCCCATCATTCTTCGTCACTACCATTTTGGCGGCAAAATTTCATCTTGGGAGCTTCCATTCATCTCTCCATTTGAATTTCCTCTTCATTTTCTTCATTGGGGAACAAGCTTTTTTCAGGAGGCGAAAGGGTAATTTTGATGGAAGGAAGTAAAGGCGAAGCAGTATTCGATTCACTGAATCTGAATCCGCAGCTCTTCATTAATGAAGCTCTCAATATTGTCGACGATTTGCTTGATGACGCTTTCGATTTCTATCAATCACAAGCATCGGCCGCCTTGAAGACCGAGGGCTCCGACAGATCCCAGGATCTTACCCTGGGAATATCGCGAGTCCGAGCGTTGGTCCAATTGGGTCTGGATAAGCGGTTGGCCATGTGGGAGAAGTACTGTCTCAATCACTGTTTTTCAGTTCCTGAAGGTTTTTCTTTACCCTCAAATGATGAATCACCTGGTGTCACTTCAATAAGTCATGACTATGACGTGGATCTGGATACAGAGTTAGATTTTTTGAGGAATAAGCTCTCTGAGGTATTGAGTGTGTCGTATGTTAATTACTTTTTGTGTTTATGTATATCATATTTCATGAGGAAATGTATTTTTCAGGCCAGAAAAGAGAATATTGTATTGAGCCAAGAGCTACAAGCTTTGGAAAGGCAAACTGCTTCCAGTAACTCCCAAATTAATCATTTCAACGAAGCATTACAACTATATGAGCAAAATTCTGTGAATGAGATGTTCCAGGAAATGATGGGAACTGCATCGGAGCTACGAGCGAAAATAGGAAAGCTGAAGAAAAGGAGGATGGAGGAAGCCAAGCTCAGTAAAGTAGAGAAGGTTCATACAAACGGAGACATCTCTCACCATCACAATGGTTTCTCTAATGCTAAGCTGGATGACATTCAAGAATTCTTAGCTGATTTGAAGACCCTT

Coding sequence (CDS)

ATGGAAGGAAGTAAAGGCGAAGCAGTATTCGATTCACTGAATCTGAATCCGCAGCTCTTCATTAATGAAGCTCTCAATATTGTCGACGATTTGCTTGATGACGCTTTCGATTTCTATCAATCACAAGCATCGGCCGCCTTGAAGACCGAGGGCTCCGACAGATCCCAGGATCTTACCCTGGGAATATCGCGAGTCCGAGCGTTGGTCCAATTGGGTCTGGATAAGCGGTTGGCCATGTGGGAGAAGTACTGTCTCAATCACTGTTTTTCAGTTCCTGAAGGTTTTTCTTTACCCTCAAATGATGAATCACCTGGTGTCACTTCAATAAGTCATGACTATGACGTGGATCTGGATACAGAGTTAGATTTTTTGAGGAATAAGCTCTCTGAGGTATTGAGTGTGTCGTATGTTAATTACTTTTTGTGTTTATGTATATCATATTTCATGAGGAAATGTATTTTTCAGGCCAGAAAAGAGAATATTGTATTGAGCCAAGAGCTACAAGCTTTGGAAAGGCAAACTGCTTCCAGTAACTCCCAAATTAATCATTTCAACGAAGCATTACAACTATATGAGCAAAATTCTGTGAATGAGATGTTCCAGGAAATGATGGGAACTGCATCGGAGCTACGAGCGAAAATAGGAAAGCTGAAGAAAAGGAGGATGGAGGAAGCCAAGCTCAGTAAAGTAGAGAAGGTTCATACAAACGGAGACATCTCTCACCATCACAATGGTTTCTCTAATGCTAAGCTGGATGACATTCAAGAATTCTTAGCTGATTTGAAGACCCTT

Protein sequence

MEGSKGEAVFDSLNLNPQLFINEALNIVDDLLDDAFDFYQSQASAALKTEGSDRSQDLTLGISRVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSISHDYDVDLDTELDFLRNKLSEVLSVSYVNYFLCLCISYFMRKCIFQARKENIVLSQELQALERQTASSNSQINHFNEALQLYEQNSVNEMFQEMMGTASELRAKIGKLKKRRMEEAKLSKVEKVHTNGDISHHHNGFSNAKLDDIQEFLADLKTL

Homology

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BLAST of CaUC06G121850 vs. NCBI nr
Match: XP_038879945.1 (protein MIS12 homolog [Benincasa hispida])

HSP 1 Score: 425.6 bits (1093), Expect = 3.1e-115
Identity = 225/264 (85.23%), Postives = 233/264 (88.26%), Query Frame = 0


Query: 1   MEGSKGEAVFDSLNLNPQLFINEALNIVDDLLDDAFDFYQSQASAALKTEGSDRSQDLTL 60
           MEGSKGEAVFDSLNLNPQLFINEALNIVDDL+DDAF FYQSQASA LKTEGSDRSQDLTL
Sbjct: 31  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFGFYQSQASATLKTEGSDRSQDLTL 90

Query: 61  GISRVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSISHDYDVDLDTE 120
           GISRVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSND+SPGVTSISHDYDV LDTE
Sbjct: 91  GISRVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDDSPGVTSISHDYDVGLDTE 150

Query: 121 LDFLRNKLSEVLSVSYVNYFLCLCISYFMRKCIFQARKENIVLSQELQALERQTASSNSQ 180
           LDFLRNKLSEV                         RKENIVL+QELQALERQTA+SNSQ
Sbjct: 151 LDFLRNKLSEV-------------------------RKENIVLNQELQALERQTATSNSQ 210

Query: 181 INHFNEALQLYEQNSVNEMFQEMMGTASELRAKIGKLKKRRMEEAKLSKVEKVHTNGDIS 240
           INHFNEALQLYEQNSVNEMFQEMMGTASELRAKIGKLKKRRMEE+KL+KVEKVHTNGDIS
Sbjct: 211 INHFNEALQLYEQNSVNEMFQEMMGTASELRAKIGKLKKRRMEESKLTKVEKVHTNGDIS 269

Query: 241 HHHNGFSNAKLDDIQEFLADLKTL 265
           H+HNG SNAKLDDI+ FL DLKTL
Sbjct: 271 HYHNGLSNAKLDDIKIFLDDLKTL 269

[Close]

BLAST of CaUC06G121850 vs. NCBI nr
Match: KAA0058004.1 (Centromere protein Mis12 [Cucumis melo var. makuwa] >TYK28378.1 Centromere protein Mis12 [Cucumis melo var. makuwa])

HSP 1 Score: 417.5 bits (1072), Expect = 8.3e-113
Identity = 219/264 (82.95%), Postives = 233/264 (88.26%), Query Frame = 0


Query: 1   MEGSKGEAVFDSLNLNPQLFINEALNIVDDLLDDAFDFYQSQASAALKTEGSDRSQDLTL 60
           MEGSKGE+VF+SLNLNPQLFINEALN VDDL+DDAFDFYQSQASAALKTEGSDRSQDLTL
Sbjct: 1   MEGSKGESVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTL 60

Query: 61  GISRVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSISHDYDVDLDTE 120
           GIS+VRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSI+HD DVDLDTE
Sbjct: 61  GISQVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSINHDQDVDLDTE 120

Query: 121 LDFLRNKLSEVLSVSYVNYFLCLCISYFMRKCIFQARKENIVLSQELQALERQTASSNSQ 180
           LD LRNKLSEV                         RKENIVL+QELQALERQTASSNSQ
Sbjct: 121 LDLLRNKLSEV-------------------------RKENIVLNQELQALERQTASSNSQ 180

Query: 181 INHFNEALQLYEQNSVNEMFQEMMGTASELRAKIGKLKKRRMEEAKLSKVEKVHTNGDIS 240
           I+HFNEALQLYEQ+SVN+MFQEM+ TASELRAKIGK+KKRR EEAKL+KVEKVHTNGDIS
Sbjct: 181 ISHFNEALQLYEQSSVNDMFQEMIKTASELRAKIGKMKKRRTEEAKLAKVEKVHTNGDIS 239

Query: 241 HHHNGFSNAKLDDIQEFLADLKTL 265
           HHHNGFSNAKLDDIQEFL+DLK +
Sbjct: 241 HHHNGFSNAKLDDIQEFLSDLKKI 239

[Close]

BLAST of CaUC06G121850 vs. NCBI nr
Match: XP_023515499.1 (protein MIS12 homolog isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 416.8 bits (1070), Expect = 1.4e-112
Identity = 220/266 (82.71%), Postives = 231/266 (86.84%), Query Frame = 0


Query: 1   MEGSKGEAVFDSLNLNPQLFINEALNIVDDLLDDAFDFYQSQASAALKTEGSDRSQDLTL 60
           MEGSKGE VFDSLNLNPQLFINEALNIVDDL+DDAFDFYQ+QASA+LKTEGSDRSQDL+L
Sbjct: 1   MEGSKGEEVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQTQASASLKTEGSDRSQDLSL 60

Query: 61  GISRVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSISHD--YDVDLD 120
           GISRVR  VQ GLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESP VTS+S D  YDVDLD
Sbjct: 61  GISRVRTFVQSGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPVVTSVSRDCLYDVDLD 120

Query: 121 TELDFLRNKLSEVLSVSYVNYFLCLCISYFMRKCIFQARKENIVLSQELQALERQTASSN 180
           TELDFLRNKLSEV                         RKEN+VL+QELQALERQTASSN
Sbjct: 121 TELDFLRNKLSEV-------------------------RKENVVLNQELQALERQTASSN 180

Query: 181 SQINHFNEALQLYEQNSVNEMFQEMMGTASELRAKIGKLKKRRMEEAKLSKVEKVHTNGD 240
           SQ+N FNEALQLYEQNSVNEMFQEMMGTASELRAKIGKLKKRRMEE+KL+KVEK+HTNGD
Sbjct: 181 SQVNRFNEALQLYEQNSVNEMFQEMMGTASELRAKIGKLKKRRMEESKLTKVEKIHTNGD 240

Query: 241 ISHHHNGFSNAKLDDIQEFLADLKTL 265
           ISHHH GFSNAKLDDIQEFLADLKTL
Sbjct: 241 ISHHHKGFSNAKLDDIQEFLADLKTL 241

[Close]

BLAST of CaUC06G121850 vs. NCBI nr
Match: KAA0058032.1 (Centromere protein Mis12 [Cucumis melo var. makuwa])

HSP 1 Score: 414.8 bits (1065), Expect = 5.4e-112
Identity = 217/264 (82.20%), Postives = 232/264 (87.88%), Query Frame = 0


Query: 1   MEGSKGEAVFDSLNLNPQLFINEALNIVDDLLDDAFDFYQSQASAALKTEGSDRSQDLTL 60
           MEGSKGE+VF+SLNLNPQLFINEALN VDDL+DDAFDFYQSQASA LKTEGSDRSQDLTL
Sbjct: 1   MEGSKGESVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASATLKTEGSDRSQDLTL 60

Query: 61  GISRVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSISHDYDVDLDTE 120
           GIS+VRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSI+HD DVDLDTE
Sbjct: 61  GISQVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSINHDQDVDLDTE 120

Query: 121 LDFLRNKLSEVLSVSYVNYFLCLCISYFMRKCIFQARKENIVLSQELQALERQTASSNSQ 180
           LD LRNKLSEV                         RKENIVL+QELQALERQTASSNSQ
Sbjct: 121 LDLLRNKLSEV-------------------------RKENIVLNQELQALERQTASSNSQ 180

Query: 181 INHFNEALQLYEQNSVNEMFQEMMGTASELRAKIGKLKKRRMEEAKLSKVEKVHTNGDIS 240
           I+HFNEALQLYEQ+SVN+MFQEM+ TASELR+KIGK+KKRR EEAKL+KVEKVHTNGDIS
Sbjct: 181 ISHFNEALQLYEQSSVNDMFQEMIKTASELRSKIGKMKKRRTEEAKLAKVEKVHTNGDIS 239

Query: 241 HHHNGFSNAKLDDIQEFLADLKTL 265
           HHHNGFSNAKLDDIQEFL+DLK +
Sbjct: 241 HHHNGFSNAKLDDIQEFLSDLKKI 239

[Close]

BLAST of CaUC06G121850 vs. NCBI nr
Match: KAG6589575.1 (Protein MIS12-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 414.1 bits (1063), Expect = 9.2e-112
Identity = 219/266 (82.33%), Postives = 229/266 (86.09%), Query Frame = 0


Query: 1   MEGSKGEAVFDSLNLNPQLFINEALNIVDDLLDDAFDFYQSQASAALKTEGSDRSQDLTL 60
           MEGSKGE VFDSLNLNPQLFINEALNIVDDL+DDAFDFYQ+QASA+LKTEGSDRSQDL L
Sbjct: 1   MEGSKGEEVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQTQASASLKTEGSDRSQDLIL 60

Query: 61  GISRVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSISHD--YDVDLD 120
           GISRVR  VQ GLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESP VTS+S D  YDVDLD
Sbjct: 61  GISRVRTFVQSGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPVVTSVSRDCLYDVDLD 120

Query: 121 TELDFLRNKLSEVLSVSYVNYFLCLCISYFMRKCIFQARKENIVLSQELQALERQTASSN 180
           TELDFLRNKLSEV                         RKEN+VL+QELQALERQTASSN
Sbjct: 121 TELDFLRNKLSEV-------------------------RKENVVLNQELQALERQTASSN 180

Query: 181 SQINHFNEALQLYEQNSVNEMFQEMMGTASELRAKIGKLKKRRMEEAKLSKVEKVHTNGD 240
           SQ+N FNEALQLYEQNSVNEMFQEMMGTASELRAKIGKLKKRRMEE+KL+KVEK+HTNGD
Sbjct: 181 SQVNRFNEALQLYEQNSVNEMFQEMMGTASELRAKIGKLKKRRMEESKLTKVEKIHTNGD 240

Query: 241 ISHHHNGFSNAKLDDIQEFLADLKTL 265
           ISHHH GFSNAKLDDIQEFLADLK L
Sbjct: 241 ISHHHKGFSNAKLDDIQEFLADLKAL 241

[Close]

BLAST of CaUC06G121850 vs. ExPASy Swiss-Prot
Match: Q2V0Z5 (Protein MIS12 homolog OS=Arabidopsis thaliana OX=3702 GN=MIS12 PE=3 SV=1)

HSP 1 Score: 200.7 bits (509), Expect = 2.1e-50
Identity = 120/272 (44.12%), Postives = 173/272 (63.60%), Query Frame = 0


Query: 1   MEGSKGEAVFDSLNLNPQLFINEALNIVDDLLDDAFDFYQSQASAALKTEGSD--RSQDL 60
           MEGSK EAVFDS+NLNPQ+FINEA+N V+D +D AFDFY   AS +LK +GSD  +SQ L
Sbjct: 1   MEGSKSEAVFDSMNLNPQIFINEAINSVEDYVDQAFDFYARDASKSLKIKGSDKQKSQAL 60

Query: 61  TLGISRVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSISHD--YDVD 120
           + GI+RVR L+   +D RL +WE Y L  CF+VP+GF LP ++ES   +S+  D  YD++
Sbjct: 61  SNGIARVRGLLLSVIDNRLKLWESYSLRFCFAVPDGFVLPKSEES---SSVHQDGLYDLE 120

Query: 121 LDTELDFLRNKLSEVLSVSYVNYFLCLCISYFMRKCIFQARKENIVLSQELQALERQTAS 180
           LD ELD LR+KL+ V                          K ++ L  ELQALER + S
Sbjct: 121 LDAELDSLRDKLNVV-------------------------GKRSVELDSELQALERSSVS 180

Query: 181 SNSQINHFNEALQLYEQNSVNEMFQEMMGTASELRAKIGKLKKRRMEEAKLSKVEKVHTN 240
               +   NEAL+LY+++S++E+F+EM   ASELRA + +LK RRM+ ++ +KV++    
Sbjct: 181 RERSLRLVNEALELYDESSMDEIFKEMTKMASELRASVERLKTRRMKASESAKVKR---- 238

Query: 241 GDISHHHNGFS----NAKLDDIQEFLADLKTL 265
             + +H   FS    + KL+D+++F A+L+ +
Sbjct: 241 --LKNHGKEFSAMTFDGKLEDLEKFQAELRKM 238

[Close]

BLAST of CaUC06G121850 vs. ExPASy TrEMBL
Match: A0A5A7US57 (Centromere protein Mis12 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold629G00040 PE=3 SV=1)

HSP 1 Score: 417.5 bits (1072), Expect = 4.0e-113
Identity = 219/264 (82.95%), Postives = 233/264 (88.26%), Query Frame = 0


Query: 1   MEGSKGEAVFDSLNLNPQLFINEALNIVDDLLDDAFDFYQSQASAALKTEGSDRSQDLTL 60
           MEGSKGE+VF+SLNLNPQLFINEALN VDDL+DDAFDFYQSQASAALKTEGSDRSQDLTL
Sbjct: 1   MEGSKGESVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTL 60

Query: 61  GISRVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSISHDYDVDLDTE 120
           GIS+VRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSI+HD DVDLDTE
Sbjct: 61  GISQVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSINHDQDVDLDTE 120

Query: 121 LDFLRNKLSEVLSVSYVNYFLCLCISYFMRKCIFQARKENIVLSQELQALERQTASSNSQ 180
           LD LRNKLSEV                         RKENIVL+QELQALERQTASSNSQ
Sbjct: 121 LDLLRNKLSEV-------------------------RKENIVLNQELQALERQTASSNSQ 180

Query: 181 INHFNEALQLYEQNSVNEMFQEMMGTASELRAKIGKLKKRRMEEAKLSKVEKVHTNGDIS 240
           I+HFNEALQLYEQ+SVN+MFQEM+ TASELRAKIGK+KKRR EEAKL+KVEKVHTNGDIS
Sbjct: 181 ISHFNEALQLYEQSSVNDMFQEMIKTASELRAKIGKMKKRRTEEAKLAKVEKVHTNGDIS 239

Query: 241 HHHNGFSNAKLDDIQEFLADLKTL 265
           HHHNGFSNAKLDDIQEFL+DLK +
Sbjct: 241 HHHNGFSNAKLDDIQEFLSDLKKI 239

[Close]

BLAST of CaUC06G121850 vs. ExPASy TrEMBL
Match: A0A5A7UQ95 (Centromere protein Mis12 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G003370 PE=3 SV=1)

HSP 1 Score: 414.8 bits (1065), Expect = 2.6e-112
Identity = 217/264 (82.20%), Postives = 232/264 (87.88%), Query Frame = 0


Query: 1   MEGSKGEAVFDSLNLNPQLFINEALNIVDDLLDDAFDFYQSQASAALKTEGSDRSQDLTL 60
           MEGSKGE+VF+SLNLNPQLFINEALN VDDL+DDAFDFYQSQASA LKTEGSDRSQDLTL
Sbjct: 1   MEGSKGESVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASATLKTEGSDRSQDLTL 60

Query: 61  GISRVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSISHDYDVDLDTE 120
           GIS+VRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSI+HD DVDLDTE
Sbjct: 61  GISQVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSINHDQDVDLDTE 120

Query: 121 LDFLRNKLSEVLSVSYVNYFLCLCISYFMRKCIFQARKENIVLSQELQALERQTASSNSQ 180
           LD LRNKLSEV                         RKENIVL+QELQALERQTASSNSQ
Sbjct: 121 LDLLRNKLSEV-------------------------RKENIVLNQELQALERQTASSNSQ 180

Query: 181 INHFNEALQLYEQNSVNEMFQEMMGTASELRAKIGKLKKRRMEEAKLSKVEKVHTNGDIS 240
           I+HFNEALQLYEQ+SVN+MFQEM+ TASELR+KIGK+KKRR EEAKL+KVEKVHTNGDIS
Sbjct: 181 ISHFNEALQLYEQSSVNDMFQEMIKTASELRSKIGKMKKRRTEEAKLAKVEKVHTNGDIS 239

Query: 241 HHHNGFSNAKLDDIQEFLADLKTL 265
           HHHNGFSNAKLDDIQEFL+DLK +
Sbjct: 241 HHHNGFSNAKLDDIQEFLSDLKKI 239

[Close]

BLAST of CaUC06G121850 vs. ExPASy TrEMBL
Match: A0A6J1JME3 (protein MIS12 homolog isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111485708 PE=3 SV=1)

HSP 1 Score: 409.1 bits (1050), Expect = 1.4e-110
Identity = 218/266 (81.95%), Postives = 227/266 (85.34%), Query Frame = 0


Query: 1   MEGSKGEAVFDSLNLNPQLFINEALNIVDDLLDDAFDFYQSQASAALKTEGSDRSQDLTL 60
           MEGSKGE VFDSLNLNPQLFINEALNIVDDL+DDAFDFYQ+QASA+LKTEGSDRSQDL L
Sbjct: 1   MEGSKGEEVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQTQASASLKTEGSDRSQDLIL 60

Query: 61  GISRVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSISHD--YDVDLD 120
           GISRVR  VQ GLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESP VTS+S D  YDVDLD
Sbjct: 61  GISRVRTFVQSGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPVVTSVSRDCLYDVDLD 120

Query: 121 TELDFLRNKLSEVLSVSYVNYFLCLCISYFMRKCIFQARKENIVLSQELQALERQTASSN 180
           TELDFLR  LSEV                         RKENIVL+QELQALERQTASSN
Sbjct: 121 TELDFLRKNLSEV-------------------------RKENIVLNQELQALERQTASSN 180

Query: 181 SQINHFNEALQLYEQNSVNEMFQEMMGTASELRAKIGKLKKRRMEEAKLSKVEKVHTNGD 240
           SQ+N FNEALQLYEQNSVNEMFQEMMGTASELRAKIGKLKKRRMEE+KL+KVEK+HTN D
Sbjct: 181 SQVNRFNEALQLYEQNSVNEMFQEMMGTASELRAKIGKLKKRRMEESKLTKVEKIHTNRD 240

Query: 241 ISHHHNGFSNAKLDDIQEFLADLKTL 265
           ISHHH GFSNAKLDDIQEFLADLKTL
Sbjct: 241 ISHHHKGFSNAKLDDIQEFLADLKTL 241

[Close]

BLAST of CaUC06G121850 vs. ExPASy TrEMBL
Match: A0A6J1E0A1 (protein MIS12 homolog OS=Cucurbita moschata OX=3662 GN=LOC111429660 PE=3 SV=1)

HSP 1 Score: 409.1 bits (1050), Expect = 1.4e-110
Identity = 217/266 (81.58%), Postives = 227/266 (85.34%), Query Frame = 0


Query: 1   MEGSKGEAVFDSLNLNPQLFINEALNIVDDLLDDAFDFYQSQASAALKTEGSDRSQDLTL 60
           MEGSKGE VFDSLNLNPQLFINEALNIVDDL+DDAFDFYQ+QASA+LKTEGSDRSQDL L
Sbjct: 1   MEGSKGEEVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQTQASASLKTEGSDRSQDLIL 60

Query: 61  GISRVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSISHD--YDVDLD 120
           GISRVR  VQ GLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESP VTS+S D  YDVDLD
Sbjct: 61  GISRVRTFVQSGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPVVTSVSRDCLYDVDLD 120

Query: 121 TELDFLRNKLSEVLSVSYVNYFLCLCISYFMRKCIFQARKENIVLSQELQALERQTASSN 180
           TELDFLRNKLSEV                         RKENIVL+QELQALERQ ASSN
Sbjct: 121 TELDFLRNKLSEV-------------------------RKENIVLNQELQALERQAASSN 180

Query: 181 SQINHFNEALQLYEQNSVNEMFQEMMGTASELRAKIGKLKKRRMEEAKLSKVEKVHTNGD 240
           SQ+N FNEALQLYE+NSVNEMFQEMMGTASELRAKIGKLKKRRMEE+KL+KVEK+HTNGD
Sbjct: 181 SQVNRFNEALQLYERNSVNEMFQEMMGTASELRAKIGKLKKRRMEESKLTKVEKIHTNGD 240

Query: 241 ISHHHNGFSNAKLDDIQEFLADLKTL 265
            SHHH GFSNAKLDDIQEFLADLK L
Sbjct: 241 ASHHHKGFSNAKLDDIQEFLADLKAL 241

[Close]

BLAST of CaUC06G121850 vs. ExPASy TrEMBL
Match: A0A6J1JD43 (protein MIS12 homolog isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111485708 PE=3 SV=1)

HSP 1 Score: 404.4 bits (1038), Expect = 3.5e-109
Identity = 218/267 (81.65%), Postives = 227/267 (85.02%), Query Frame = 0


Query: 1   MEGSKGEAVFDSLNLNPQLFINEALNIVDDLLDDAFDFYQSQASAALKTEGSDRSQDLTL 60
           MEGSKGE VFDSLNLNPQLFINEALNIVDDL+DDAFDFYQ+QASA+LKTEGSDRSQDL L
Sbjct: 1   MEGSKGEEVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQTQASASLKTEGSDRSQDLIL 60

Query: 61  GISRVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSISHD--YDVDLD 120
           GISRVR  VQ GLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESP VTS+S D  YDVDLD
Sbjct: 61  GISRVRTFVQSGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPVVTSVSRDCLYDVDLD 120

Query: 121 TELDFLRNKLSEVLSVSYVNYFLCLCISYFMRKCIFQARKENIVLSQELQALERQTASSN 180
           TELDFLR  LSEV                         RKENIVL+QELQALERQTASSN
Sbjct: 121 TELDFLRKNLSEV-------------------------RKENIVLNQELQALERQTASSN 180

Query: 181 SQINHFNEALQLYEQNSVNEMFQ-EMMGTASELRAKIGKLKKRRMEEAKLSKVEKVHTNG 240
           SQ+N FNEALQLYEQNSVNEMFQ EMMGTASELRAKIGKLKKRRMEE+KL+KVEK+HTN 
Sbjct: 181 SQVNRFNEALQLYEQNSVNEMFQAEMMGTASELRAKIGKLKKRRMEESKLTKVEKIHTNR 240

Query: 241 DISHHHNGFSNAKLDDIQEFLADLKTL 265
           DISHHH GFSNAKLDDIQEFLADLKTL
Sbjct: 241 DISHHHKGFSNAKLDDIQEFLADLKTL 242

[Close]

BLAST of CaUC06G121850 vs. TAIR 10
Match: AT5G35520.1 (minichromosome instability 12 (mis12)-like )

HSP 1 Score: 200.7 bits (509), Expect = 1.5e-51
Identity = 120/272 (44.12%), Postives = 173/272 (63.60%), Query Frame = 0


Query: 1   MEGSKGEAVFDSLNLNPQLFINEALNIVDDLLDDAFDFYQSQASAALKTEGSD--RSQDL 60
           MEGSK EAVFDS+NLNPQ+FINEA+N V+D +D AFDFY   AS +LK +GSD  +SQ L
Sbjct: 1   MEGSKSEAVFDSMNLNPQIFINEAINSVEDYVDQAFDFYARDASKSLKIKGSDKQKSQAL 60

Query: 61  TLGISRVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSISHD--YDVD 120
           + GI+RVR L+   +D RL +WE Y L  CF+VP+GF LP ++ES   +S+  D  YD++
Sbjct: 61  SNGIARVRGLLLSVIDNRLKLWESYSLRFCFAVPDGFVLPKSEES---SSVHQDGLYDLE 120

Query: 121 LDTELDFLRNKLSEVLSVSYVNYFLCLCISYFMRKCIFQARKENIVLSQELQALERQTAS 180
           LD ELD LR+KL+ V                          K ++ L  ELQALER + S
Sbjct: 121 LDAELDSLRDKLNVV-------------------------GKRSVELDSELQALERSSVS 180

Query: 181 SNSQINHFNEALQLYEQNSVNEMFQEMMGTASELRAKIGKLKKRRMEEAKLSKVEKVHTN 240
               +   NEAL+LY+++S++E+F+EM   ASELRA + +LK RRM+ ++ +KV++    
Sbjct: 181 RERSLRLVNEALELYDESSMDEIFKEMTKMASELRASVERLKTRRMKASESAKVKR---- 238

Query: 241 GDISHHHNGFS----NAKLDDIQEFLADLKTL 265
             + +H   FS    + KL+D+++F A+L+ +
Sbjct: 241 --LKNHGKEFSAMTFDGKLEDLEKFQAELRKM 238

The following BLAST results are available for this feature:

BLAST of CaUC06G121850 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038879945.1	3.1e-115	85.23	protein MIS12 homolog [Benincasa hispida]	[more]
KAA0058004.1	8.3e-113	82.95	Centromere protein Mis12 [Cucumis melo var. makuwa] >TYK28378.1 Centromere prote...	[more]
XP_023515499.1	1.4e-112	82.71	protein MIS12 homolog isoform X2 [Cucurbita pepo subsp. pepo]	[more]
KAA0058032.1	5.4e-112	82.20	Centromere protein Mis12 [Cucumis melo var. makuwa]	[more]
KAG6589575.1	9.2e-112	82.33	Protein MIS12-like protein, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of CaUC06G121850 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q2V0Z5	2.1e-50	44.12	Protein MIS12 homolog OS=Arabidopsis thaliana OX=3702 GN=MIS12 PE=3 SV=1	[more]

BLAST of CaUC06G121850 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7US57	4.0e-113	82.95	Centromere protein Mis12 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol...	[more]
A0A5A7UQ95	2.6e-112	82.20	Centromere protein Mis12 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffol...	[more]
A0A6J1JME3	1.4e-110	81.95	protein MIS12 homolog isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111485708 PE=...	[more]
A0A6J1E0A1	1.4e-110	81.58	protein MIS12 homolog OS=Cucurbita moschata OX=3662 GN=LOC111429660 PE=3 SV=1	[more]
A0A6J1JD43	3.5e-109	81.65	protein MIS12 homolog isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111485708 PE=...	[more]

BLAST of CaUC06G121850 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G35520.1	1.5e-51	44.12	minichromosome instability 12 (mis12)-like	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (USVL246-FR2) v1
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 153..173
IPR008685	Centromere protein Mis12	PFAM	PF05859	Mis12	coord: 11..133 e-value: 4.7E-18 score: 65.6
IPR008685	Centromere protein Mis12	PANTHER	PTHR14527	PROTEIN MIS12 HOMOLOG	coord: 1..131 coord: 150..263

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CaUC06G121850.1	CaUC06G121850.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051301	cell division
biological_process	GO:0051382	kinetochore assembly
biological_process	GO:0000070	mitotic sister chromatid segregation
biological_process	GO:0034501	protein localization to kinetochore
biological_process	GO:0000278	mitotic cell cycle
cellular_component	GO:0000818	nuclear MIS12/MIND complex
cellular_component	GO:0000775	chromosome, centromeric region
cellular_component	GO:0005634	nucleus