CaUC03G061730 (gene) Watermelon (USVL246-FR2) v1

Overview
NameCaUC03G061730
Typegene
OrganismCitrullus amarus (Watermelon (USVL246-FR2) v1)
DescriptionIsoleucine N-monooxygenase 1
LocationCiama_Chr03: 25607557 .. 25609229 (+)
RNA-Seq ExpressionCaUC03G061730
SyntenyCaUC03G061730
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTGAGGAACAAAAACTCACCAACACACCAATGGATTCATGCAATAATGAAGCAAGTCAACACTGAAATTGCTTGCATTCGTCTCGGATCAAATACCCACATAATCCCTGTCACTTCTCCAGAACTCGCTCTTGAATTTTTAAAGACGCATGATTCTGTTTTTGTACCACGACCCATTTCCATGACTGCCGAGATATTGAGCCGTGGATATCTAAGTGCAGTTCTTTCACCCACGGGGGATCAATGGAAGAAGATGAGAAGGATACTCGTTTCTCAAGTAACCAATCCTCCGACTCTCCATCGAATGCTTGGCCGGAGAACGAAGAAACCTGATACTCTCCTACACTACATTTTTAGCCTCACTAGAAATAGTGAAGCGATCAACATAAGAAGCATTACACAACATTATTGCGGTAACACTATTAGAAAAATGGTGTTCAATGGAAGATACTATGGAAAAGGGAGGGAACATGGGGGGCCAACTTTTGAAGAAGAGAACCATAATCAAGCACTGTTTACAGTTCTAAAGCATCTTAATGCATTTTCCATATTAGATTTTATGCCTTGCTTGAAGCCATTTGATCTGGGTGGACACGAGAAAATAGTAAAAAGGGCTTTTAAAGTCATAAGAGATCTTGACGAACCCATTATAGACAAGAGAGTACAAGAGTGGAGAGATGAGAAGAAAAAGGAGGCTGAGGACATTCTTGATATCCTTATCTCACTAAAAAATGAGAATGGCAAATAGCTATTGTCAATTGAAGAGATTAAGGCTCAAGTCACGGTAATGTATAACACTTTCTTCATTATATTTTTGTAGAGTATCCTACATTAAAAAGATGAAGAGAGATCTTATCATTTTCTTTGTAAGATATGGGAGTTATTCCTCTCATTATAGCCCATTGCTTTTTATGTAAAAAATCATGTATTTCTAATGTGGTATCAAAGAGCGTGAAGCCCAAACAGGTATTTGGTCCATACTTGAGATCCAATTCAAGAATGGTGAATTCATAGAGACACCATCTTGAGGATGACATGTTCAGGATCTTGTCATAAAAAAGATGAAGAAACTTCACAATTTTTATAAGATACATGTATTAATTAGTTTTATTTATTACCAATTGGTTTTGAGATGGAACCTCATGCTTACCTTATTCATATATGGCACACACAAACAAAGTTGAAGCATTTCCTTATGTTAATGAAATTATTTTTTTTCCCGTCATATGACAGGAGTTGCAAATTGCAACCATAGACAATCCTTCAAACACTGTGGAATGTGCAATGGCAGAATTACTCAATCAACCAAAAATTCTCCAAAAGGATGTTGAAGAATTGGACCAAGTAGTTGGAAGAAATAGGTTGGTTCAAGAATCGGATATACCTAATCTCAAGTATCTCATAGCTTGTACTAGAGAATCTTTTCGACTTCATCCATTTTCACCCTTCAATGTTCCTCATGTTTCCAACTGTGACATTGTTGTGGCTGGCTACTTCGTCCCCAAAGGTAGCCAAGTTTTGTTATCTCGTCTAGGCTTTGGCCAAAACCCAAGGATTTGGGAGGATCCGATGAGATTTGATCCAAAATGTCATCTAAAAAATGGTACACTTGAATTAGGAATTTCAGAACCCAGCCTACAATTCATCACCTTCACTAGAGGGAGGAGATGA

mRNA sequence

ATGTTGAGGAACAAAAACTCACCAACACACCAATGGATTCATGCAATAATGAAGCAAGTCAACACTGAAATTGCTTGCATTCGTCTCGGATCAAATACCCACATAATCCCTGTCACTTCTCCAGAACTCGCTCTTGAATTTTTAAAGACGCATGATTCTGTTTTTGTACCACGACCCATTTCCATGACTGCCGAGATATTGAGCCGTGGATATCTAAGTGCAGTTCTTTCACCCACGGGGGATCAATGGAAGAAGATGAGAAGGATACTCGTTTCTCAAGTAACCAATCCTCCGACTCTCCATCGAATGCTTGGCCGGAGAACGAAGAAACCTGATACTCTCCTACACTACATTTTTAGCCTCACTAGAAATAGTGAAGCGATCAACATAAGAAGCATTACACAACATTATTGCGGTAACACTATTAGAAAAATGGTGTTCAATGGAAGATACTATGGAAAAGGGAGGGAACATGGGGGGCCAACTTTTGAAGAAGAGAACCATAATCAAGCACTGTTTACAGTTCTAAAGCATCTTAATGCATTTTCCATATTAGATTTTATGCCTTGCTTGAAGCCATTTGATCTGGGTGGACACGAGAAAATAGAGTTGCAAATTGCAACCATAGACAATCCTTCAAACACTGTGGAATGTGCAATGGCAGAATTACTCAATCAACCAAAAATTCTCCAAAAGGATGTTGAAGAATTGGACCAAGTAGTTGGAAGAAATAGGTTGGTTCAAGAATCGGATATACCTAATCTCAAGTATCTCATAGCTTGTACTAGAGAATCTTTTCGACTTCATCCATTTTCACCCTTCAATGTTCCTCATGTTTCCAACTGTGACATTGTTGTGGCTGGCTACTTCGTCCCCAAAGGTAGCCAAGTTTTGTTATCTCGTCTAGGCTTTGGCCAAAACCCAAGGATTTGGGAGGATCCGATGAGATTTGATCCAAAATGTCATCTAAAAAATGGTACACTTGAATTAGGAATTTCAGAACCCAGCCTACAATTCATCACCTTCACTAGAGGGAGGAGATGA

Coding sequence (CDS)

ATGTTGAGGAACAAAAACTCACCAACACACCAATGGATTCATGCAATAATGAAGCAAGTCAACACTGAAATTGCTTGCATTCGTCTCGGATCAAATACCCACATAATCCCTGTCACTTCTCCAGAACTCGCTCTTGAATTTTTAAAGACGCATGATTCTGTTTTTGTACCACGACCCATTTCCATGACTGCCGAGATATTGAGCCGTGGATATCTAAGTGCAGTTCTTTCACCCACGGGGGATCAATGGAAGAAGATGAGAAGGATACTCGTTTCTCAAGTAACCAATCCTCCGACTCTCCATCGAATGCTTGGCCGGAGAACGAAGAAACCTGATACTCTCCTACACTACATTTTTAGCCTCACTAGAAATAGTGAAGCGATCAACATAAGAAGCATTACACAACATTATTGCGGTAACACTATTAGAAAAATGGTGTTCAATGGAAGATACTATGGAAAAGGGAGGGAACATGGGGGGCCAACTTTTGAAGAAGAGAACCATAATCAAGCACTGTTTACAGTTCTAAAGCATCTTAATGCATTTTCCATATTAGATTTTATGCCTTGCTTGAAGCCATTTGATCTGGGTGGACACGAGAAAATAGAGTTGCAAATTGCAACCATAGACAATCCTTCAAACACTGTGGAATGTGCAATGGCAGAATTACTCAATCAACCAAAAATTCTCCAAAAGGATGTTGAAGAATTGGACCAAGTAGTTGGAAGAAATAGGTTGGTTCAAGAATCGGATATACCTAATCTCAAGTATCTCATAGCTTGTACTAGAGAATCTTTTCGACTTCATCCATTTTCACCCTTCAATGTTCCTCATGTTTCCAACTGTGACATTGTTGTGGCTGGCTACTTCGTCCCCAAAGGTAGCCAAGTTTTGTTATCTCGTCTAGGCTTTGGCCAAAACCCAAGGATTTGGGAGGATCCGATGAGATTTGATCCAAAATGTCATCTAAAAAATGGTACACTTGAATTAGGAATTTCAGAACCCAGCCTACAATTCATCACCTTCACTAGAGGGAGGAGATGA

Protein sequence

MLRNKNSPTHQWIHAIMKQVNTEIACIRLGSNTHIIPVTSPELALEFLKTHDSVFVPRPISMTAEILSRGYLSAVLSPTGDQWKKMRRILVSQVTNPPTLHRMLGRRTKKPDTLLHYIFSLTRNSEAINIRSITQHYCGNTIRKMVFNGRYYGKGREHGGPTFEEENHNQALFTVLKHLNAFSILDFMPCLKPFDLGGHEKIELQIATIDNPSNTVECAMAELLNQPKILQKDVEELDQVVGRNRLVQESDIPNLKYLIACTRESFRLHPFSPFNVPHVSNCDIVVAGYFVPKGSQVLLSRLGFGQNPRIWEDPMRFDPKCHLKNGTLELGISEPSLQFITFTRGRR
Homology
BLAST of CaUC03G061730 vs. NCBI nr
Match: KAA0067056.1 (isoleucine N-monooxygenase 1 [Cucumis melo var. makuwa] >TYK26293.1 isoleucine N-monooxygenase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 540.0 bits (1390), Expect = 1.5e-149
Identity = 267/407 (65.60%), Postives = 306/407 (75.18%), Query Frame = 0

Query: 1   MLRNKNSPTHQWIHAIMKQVNTEIACIRLGSNTHIIPVTSPELALEFLKTHDSVFVPRPI 60
           MLRNKN PT+QWIH +MKQ NTEIACIRLGSNTHIIPV SPEL+LEFL THDSVF  R I
Sbjct: 51  MLRNKNLPTYQWIHEVMKQFNTEIACIRLGSNTHIIPVASPELSLEFLNTHDSVFGSRSI 110

Query: 61  SMTAEILSRGYLSAVLSPTGDQWKKMRRILVSQVTNPPTLHRMLGRRTKKPDTLLHYIFS 120
           SMTAEI+S GYLS VLSP G+QWKKMR+ILVSQV NPPTLH+M+G+RT + D LL YIF 
Sbjct: 111 SMTAEIVSNGYLSTVLSPMGEQWKKMRKILVSQVLNPPTLHQMVGQRTDEADILLRYIFG 170

Query: 121 LTRNSEAINIRSITQHYCGNTIRKMVFNGRYYGKGREHGGPTFEEENHNQALFTVLKHLN 180
           LTRN EAINIRSI +HYCG  IR+M+F+ RYYGKGRE GGP+ EEE+HNQAL ++L+H+N
Sbjct: 171 LTRNGEAINIRSIVRHYCGTVIRRMIFSRRYYGKGREDGGPSLEEEDHNQALLSILRHVN 230

Query: 181 AFSILDFMPCLKPFDLGGHEKI-------------------------------------- 240
           AFSI +F+P LK FDL GHEKI                                      
Sbjct: 231 AFSISEFIPLLKKFDLDGHEKIVKRALKVIRNNDEPIIEERVQDWRDGKKKKAEDILDIL 290

Query: 241 ----------------------ELQIATIDNPSNTVECAMAELLNQPKILQKDVEELDQV 300
                                 +LQ+A+IDNPSN VE A+AELLNQPKI+QK VEELD+V
Sbjct: 291 ISLKNENGKSLLSIEEIKAQVTDLQLASIDNPSNAVEWALAELLNQPKIIQKAVEELDKV 350

Query: 301 VGRNRLVQESDIPNLKYLIACTRESFRLHPFSPFNVPHVSNCDIVVAGYFVPKGSQVLLS 348
           +GR+RLVQE DIPNL YL AC RESFRLHPFSPFNVPHVSN D+ VAGYF+P+GSQVLLS
Sbjct: 351 IGRDRLVQECDIPNLMYLTACIRESFRLHPFSPFNVPHVSNSDVFVAGYFIPRGSQVLLS 410

BLAST of CaUC03G061730 vs. NCBI nr
Match: XP_011655052.1 (isoleucine N-monooxygenase 1 [Cucumis sativus] >KAE8648059.1 hypothetical protein Csa_005885 [Cucumis sativus])

HSP 1 Score: 527.7 bits (1358), Expect = 7.5e-146
Identity = 262/407 (64.37%), Postives = 301/407 (73.96%), Query Frame = 0

Query: 1   MLRNKNSPTHQWIHAIMKQVNTEIACIRLGSNTHIIPVTSPELALEFLKTHDSVFVPRPI 60
           ML NKN P +QWIH +MKQ NTEIACIRLGSNTHIIPV SPEL+LEFL THDSVF  R I
Sbjct: 51  MLSNKNLPAYQWIHEVMKQFNTEIACIRLGSNTHIIPVASPELSLEFLNTHDSVFGSRSI 110

Query: 61  SMTAEILSRGYLSAVLSPTGDQWKKMRRILVSQVTNPPTLHRMLGRRTKKPDTLLHYIFS 120
           SMTAEI+S GYLS VLSP G+QWKKMR+IL SQV N  TLHRMLG+RT + D LL YIF 
Sbjct: 111 SMTAEIVSNGYLSTVLSPMGEQWKKMRKILASQVLNSSTLHRMLGQRTDEADILLRYIFG 170

Query: 121 LTRNSEAINIRSITQHYCGNTIRKMVFNGRYYGKGREHGGPTFEEENHNQALFTVLKHLN 180
           LT+N EAINIRSI +HYCG  IR+M+F+ RYYGKGRE GGP+ EEE HNQAL ++L+H+N
Sbjct: 171 LTKNGEAINIRSIVRHYCGTVIRRMIFSRRYYGKGREDGGPSLEEEEHNQALLSILRHVN 230

Query: 181 AFSILDFMPCLKPFDLGGHEKI-------------------------------------- 240
           AFSI +F+P LK FDL GH KI                                      
Sbjct: 231 AFSISEFIPLLKTFDLDGHGKIVKRALKVIRNHDEPIIEERVQEWGDGKKKKVEDILDIL 290

Query: 241 ----------------------ELQIATIDNPSNTVECAMAELLNQPKILQKDVEELDQV 300
                                 +LQ+A+IDNPSN VE A+AELLNQPKI+Q+ +EELD+V
Sbjct: 291 ISLKNENGKSLLSIEEIKAQVTDLQLASIDNPSNAVEWALAELLNQPKIIQQALEELDKV 350

Query: 301 VGRNRLVQESDIPNLKYLIACTRESFRLHPFSPFNVPHVSNCDIVVAGYFVPKGSQVLLS 348
           +GR+RLVQESDIPNLKYL AC RESFRLHPFSPFNVPHVSN D+ VAGYF+PKGSQVLLS
Sbjct: 351 IGRDRLVQESDIPNLKYLTACIRESFRLHPFSPFNVPHVSNSDVFVAGYFIPKGSQVLLS 410

BLAST of CaUC03G061730 vs. NCBI nr
Match: XP_038880438.1 (tryptophan N-monooxygenase CYP79A68-like [Benincasa hispida])

HSP 1 Score: 510.8 bits (1314), Expect = 9.5e-141
Identity = 263/411 (63.99%), Postives = 302/411 (73.48%), Query Frame = 0

Query: 1   MLRNKNSPTHQWIHAIMKQVNTEIACIRLGSNTHIIPVTSPELALEFLKTHDSVFVPRPI 60
           ML NK SPTHQWIH++MK++NTEIA IRLG NT++IPVTSPELALEFLKT+DSVF  R  
Sbjct: 72  MLINK-SPTHQWIHSVMKKLNTEIASIRLG-NTYVIPVTSPELALEFLKTYDSVFASRSS 131

Query: 61  SMTA-EILSRGYLSAVLSPTGDQWKKMRRILVSQVTNPPTLHRMLGRRTKKPDTLLHYIF 120
           +  A  IL+RGY+S +LSP GDQWKKMRRIL S++ NP TLHRMLG+RT + DTLL YIF
Sbjct: 132 TSNAIYILTRGYVSTILSPLGDQWKKMRRILTSKILNPSTLHRMLGQRTAEADTLLRYIF 191

Query: 121 SLTRNSE---AINIRSITQHYCGNTIRKMVFNGRYYGKGREHGGPTFEEENHNQALFTVL 180
           + T N+     IN+RSITQHYCGN +R+M+FN RYYGKGRE GGPTFEEE H QAL T++
Sbjct: 192 TQTNNNRGGAVINLRSITQHYCGNILRQMLFNRRYYGKGREDGGPTFEEEEHVQALLTIV 251

Query: 181 KHLNAFSILDFMPCLKPFDLGGHEKI---------------------------------- 240
           KH++AFSI DFMPCLK FDL GH+KI                                  
Sbjct: 252 KHIHAFSISDFMPCLKSFDLDGHQKIMKNALNILQKYDEPIIDERVQEWKDKKIKEVEDI 311

Query: 241 --------------------------ELQIATIDNPSNTVECAMAELLNQPKILQKDVEE 300
                                     +LQIATIDNPSN VE AMAELLNQPKIL+K VEE
Sbjct: 312 LDILISLKDENTNSLLSIEEIKAQITDLQIATIDNPSNAVEWAMAELLNQPKILEKVVEE 371

Query: 301 LDQVVGRNRLVQESDIPNLKYLIACTRESFRLHPFSPFNVPHVSNCDIVVAGYFVPKGSQ 348
           LD+VVGR RLVQESDIP LKYL AC RESFRLHPFS FNVPH+S  DIVVAGYF+PKGS+
Sbjct: 372 LDKVVGRERLVQESDIPKLKYLTACARESFRLHPFSSFNVPHISTSDIVVAGYFIPKGSE 431

BLAST of CaUC03G061730 vs. NCBI nr
Match: XP_008450287.1 (PREDICTED: phenylalanine N-monooxygenase-like isoform X1 [Cucumis melo])

HSP 1 Score: 507.7 bits (1306), Expect = 8.0e-140
Identity = 254/403 (63.03%), Postives = 289/403 (71.71%), Query Frame = 0

Query: 5   KNSPTHQWIHAIMKQVNTEIACIRLGSNTHIIPVTSPELALEFLKTHDSVFVPRPISMTA 64
           K SP HQWIH +MKQ+NTEIAC  LGSNTHII VTSPELALEFLKTHDS F  RPI+ T 
Sbjct: 77  KRSPRHQWIHTVMKQLNTEIACFHLGSNTHIIIVTSPELALEFLKTHDSTFASRPIASTT 136

Query: 65  EILSRGYLSAVLSPTGDQWKKMRRILVSQVTNPPTLHRMLGRRTKKPDTLLHYIFSLTRN 124
           EILS G+LS VLSP GDQWKKM+RIL S V NP TLH+M G+R K+ D LLHYIF     
Sbjct: 137 EILSCGFLSTVLSPMGDQWKKMKRILASHVLNPSTLHQMRGQRIKEADALLHYIFDHASR 196

Query: 125 SEAINIRSITQHYCGNTIRKMVFNGRYYGKGREHGGPTFEEENHNQALFTVLKHLNAFSI 184
            EAINIRSITQHYC N IR+M+FN RYY KGRE+ GP+ EEE++NQAL T+L HL +FSI
Sbjct: 197 CEAINIRSITQHYCSNIIRRMIFNRRYYKKGRENEGPSLEEEDYNQALLTILMHLYSFSI 256

Query: 185 LDFMPCLKPFDLGGHEKI------------------------------------------ 244
            +++ CLKPFDLGGH+KI                                          
Sbjct: 257 SEYIACLKPFDLGGHQKIIRKALKVITNHDGPIIEERVQEWRDGKKKEVQDILDILITVK 316

Query: 245 ------------------ELQIATIDNPSNTVECAMAELLNQPKILQKDVEELDQVVGRN 304
                             ELQ+ATIDNPSN VE AM ELLNQPKILQK VEELD+VVGR+
Sbjct: 317 KENGESLLSIEEIKAQVTELQLATIDNPSNAVEWAMIELLNQPKILQKVVEELDEVVGRD 376

Query: 305 RLVQESDIPNLKYLIACTRESFRLHPFSPFNVPHVSNCDIVVAGYFVPKGSQVLLSRLGF 348
           RLVQE DIPNLKYL AC RESFRLHPFSPFN+PHVS  DIVV GYF+PKGSQ+LL R+G 
Sbjct: 377 RLVQEFDIPNLKYLTACMRESFRLHPFSPFNLPHVSISDIVVGGYFIPKGSQILLCRVGL 436

BLAST of CaUC03G061730 vs. NCBI nr
Match: XP_008450288.1 (PREDICTED: phenylalanine N-monooxygenase-like isoform X2 [Cucumis melo])

HSP 1 Score: 507.7 bits (1306), Expect = 8.0e-140
Identity = 254/403 (63.03%), Postives = 289/403 (71.71%), Query Frame = 0

Query: 5   KNSPTHQWIHAIMKQVNTEIACIRLGSNTHIIPVTSPELALEFLKTHDSVFVPRPISMTA 64
           K SP HQWIH +MKQ+NTEIAC  LGSNTHII VTSPELALEFLKTHDS F  RPI+ T 
Sbjct: 77  KRSPRHQWIHTVMKQLNTEIACFHLGSNTHIIIVTSPELALEFLKTHDSTFASRPIASTT 136

Query: 65  EILSRGYLSAVLSPTGDQWKKMRRILVSQVTNPPTLHRMLGRRTKKPDTLLHYIFSLTRN 124
           EILS G+LS VLSP GDQWKKM+RIL S V NP TLH+M G+R K+ D LLHYIF     
Sbjct: 137 EILSCGFLSTVLSPMGDQWKKMKRILASHVLNPSTLHQMRGQRIKEADALLHYIFDHASR 196

Query: 125 SEAINIRSITQHYCGNTIRKMVFNGRYYGKGREHGGPTFEEENHNQALFTVLKHLNAFSI 184
            EAINIRSITQHYC N IR+M+FN RYY KGRE+ GP+ EEE++NQAL T+L HL +FSI
Sbjct: 197 CEAINIRSITQHYCSNIIRRMIFNRRYYKKGRENEGPSLEEEDYNQALLTILMHLYSFSI 256

Query: 185 LDFMPCLKPFDLGGHEKI------------------------------------------ 244
            +++ CLKPFDLGGH+KI                                          
Sbjct: 257 SEYIACLKPFDLGGHQKIIRKALKVITNHDGPIIEERVQEWRDGKKKEVQDILDILITVK 316

Query: 245 ------------------ELQIATIDNPSNTVECAMAELLNQPKILQKDVEELDQVVGRN 304
                             ELQ+ATIDNPSN VE AM ELLNQPKILQK VEELD+VVGR+
Sbjct: 317 KENGESLLSIEEIKAQVTELQLATIDNPSNAVEWAMIELLNQPKILQKVVEELDEVVGRD 376

Query: 305 RLVQESDIPNLKYLIACTRESFRLHPFSPFNVPHVSNCDIVVAGYFVPKGSQVLLSRLGF 348
           RLVQE DIPNLKYL AC RESFRLHPFSPFN+PHVS  DIVV GYF+PKGSQ+LL R+G 
Sbjct: 377 RLVQEFDIPNLKYLTACMRESFRLHPFSPFNLPHVSISDIVVGGYFIPKGSQILLCRVGL 436

BLAST of CaUC03G061730 vs. ExPASy Swiss-Prot
Match: A0A068Q605 (Tryptophan N-monooxygenase CYP79A68 OS=Prunus mume OX=102107 GN=CYP79A68 PE=1 SV=1)

HSP 1 Score: 370.9 bits (951), Expect = 1.5e-101
Identity = 181/407 (44.47%), Postives = 255/407 (62.65%), Query Frame = 0

Query: 5   KNSPTHQWIHAIMKQVNTEIACIRLGSNTHIIPVTSPELALEFLKTHDSVFVPRPISMTA 64
           +N P H+WIH++MK++NT+IACIRLG N H+IPVTSPE+A EFLK +D+VF  RP++M  
Sbjct: 64  RNRPAHRWIHSLMKKLNTDIACIRLG-NVHVIPVTSPEIAREFLKKNDAVFASRPVTMAT 123

Query: 65  EILSRGYLSAVLSPTGDQWKKMRRILVSQVTNPPTLHRMLGRRTKKPDTLLHYIF---SL 124
           + LS GYL+ V+ P GDQW+KMRR+LV++  NP  +H +LG+R ++ D L+ +++   S 
Sbjct: 124 KTLSSGYLTTVVGPWGDQWRKMRRVLVAEAFNPSRVHWLLGKRNEEADNLVKFLYNQCSA 183

Query: 125 TRNSEAINIRSITQHYCGNTIRKMVFNGRYYGKGREHGGPTFEEENHNQALFTVLKHLNA 184
            +N   +N+R   Q Y G+ +RKM+FN  Y+GKGRE GGP  EEE H  AL T+L +  A
Sbjct: 184 NQNGAVVNVRIAAQFYSGSIMRKMIFNRTYFGKGREDGGPGVEEEEHVSALLTLLTYAYA 243

Query: 185 FSILDFMPCLKPFDLGGHEK---------------------------------------- 244
           F + D++P L+ FD+ GHEK                                        
Sbjct: 244 FCVSDYLPWLRVFDIDGHEKKVRKAMNIVKKHQEPIVNERLQEWRDGKRNEPDDLLDVFI 303

Query: 245 --------------------IELQIATIDNPSNTVECAMAELLNQPKILQKDVEELDQVV 304
                                ELQ++T+D+P N  E A+ E+LNQP++L+K  EELD+VV
Sbjct: 304 SLKDANGQPLLSDEEIKAQTTELQLSTVDSPFNIAEWALTEMLNQPEMLKKAEEELDRVV 363

Query: 305 GRNRLVQESDIPNLKYLIACTRESFRLHPFSPFNVPHVSNCDIVVAGYFVPKGSQVLLSR 348
           G+  LVQES +P+L Y+ AC +E  RLHP  PFN+PHVS  D  VAGYF+PKGS V+LSR
Sbjct: 364 GKKTLVQESHVPHLPYIRACAKEVMRLHPVGPFNLPHVSIADAEVAGYFIPKGSNVILSR 423

BLAST of CaUC03G061730 vs. ExPASy Swiss-Prot
Match: Q9FLC8 (Phenylalanine N-monooxygenase OS=Arabidopsis thaliana OX=3702 GN=CYP79A2 PE=1 SV=1)

HSP 1 Score: 357.1 bits (915), Expect = 2.3e-97
Identity = 181/409 (44.25%), Postives = 251/409 (61.37%), Query Frame = 0

Query: 5   KNSPTHQWIHAIMKQVNTEIACIRLGSNTHIIPVTSPELALEFLKTHDSVFVPRPISMTA 64
           +N P  +WIH++MK++NT+IACIRL +NTH+IPVTSP +A E LK  DSVF  RP++M  
Sbjct: 59  RNKPVFRWIHSLMKELNTDIACIRL-ANTHVIPVTSPRIAREILKKQDSVFATRPLTMGT 118

Query: 65  EILSRGYLSAVLSPTGDQWKKMRRILVSQVTNPPTLHRMLGRRTKKPDTLLHYIFSLTRN 124
           E  SRGYL+  + P G+QWKKMRR++ S VT+  +   ML +RT++ D L+ YI + +  
Sbjct: 119 EYCSRGYLTVAVEPQGEQWKKMRRVVASHVTSKKSFQMMLQKRTEEADNLVRYINNRSVK 178

Query: 125 SE-----AINIRSITQHYCGNTIRKMVFNGRYYGKGREHG-GPTFEEENHNQALFTVLKH 184
           +       I++R   + Y GN  RKM+F  R++GKG E G GP  EE  H ++LFTVL H
Sbjct: 179 NRGNAFVVIDLRLAVRQYSGNVARKMMFGIRHFGKGSEDGSGPGLEEIEHVESLFTVLTH 238

Query: 185 LNAFSILDFMPCLKPFDLGGHEKI------------------------------------ 244
           L AF++ D++P L+  DL GHEK+                                    
Sbjct: 239 LYAFALSDYVPWLRFLDLEGHEKVVSNAMRNVSKYNDPFVDERLMQWRNGKMKEPQDFLD 298

Query: 245 ------------------------ELQIATIDNPSNTVECAMAELLNQPKILQKDVEELD 304
                                   EL +AT+DNPSN  E  MAE++N+P I+QK VEE+D
Sbjct: 299 MFIIAKDTDGKPTLSDEEIKAQVTELMLATVDNPSNAAEWGMAEMINEPSIMQKAVEEID 358

Query: 305 QVVGRNRLVQESDIPNLKYLIACTRESFRLHPFSPFNVPHVSNCDIVVAGYFVPKGSQVL 348
           +VVG++RLV ESD+PNL Y+ AC +E+FRLHP +PFN+PH+S  D VV GYF+PKGS VL
Sbjct: 359 RVVGKDRLVIESDLPNLNYVKACVKEAFRLHPVAPFNLPHMSTTDTVVDGYFIPKGSHVL 418

BLAST of CaUC03G061730 vs. ExPASy Swiss-Prot
Match: Q6J541 (Isoleucine N-monooxygenase 1 OS=Lotus japonicus OX=34305 GN=CYP79D3 PE=1 SV=1)

HSP 1 Score: 348.2 bits (892), Expect = 1.1e-94
Identity = 179/407 (43.98%), Postives = 244/407 (59.95%), Query Frame = 0

Query: 6   NSPTHQWIHAIMKQVNTEIACIRLGSNTHIIPVTSPELALEFLKTHDSVFVPRPISMTAE 65
           N P   WIH +MK++NTEIACIRL +NT +IPVT P +A EFLK HD+ F  RP  M+ +
Sbjct: 64  NRPATIWIHKLMKEMNTEIACIRL-ANTIVIPVTCPTIACEFLKKHDASFASRPKIMSTD 123

Query: 66  ILSRGYLSAVLSPTGDQWKKMRRILVSQVTNPPTLHRMLGRRTKKPDTLLHYIFS----- 125
           I S G+++ VL P G+QWKKM+R+LV+ + +P     +LG+R ++ D L+ YI++     
Sbjct: 124 IASDGFITTVLVPYGEQWKKMKRVLVNNLLSPQKHQWLLGKRNEEADNLMFYIYNKCCKD 183

Query: 126 LTRNSEAINIRSITQHYCGNTIRKMVFNGRYYGKGREHGGPTFEEENHNQALFTVLKHLN 185
           +      +NIR   QHY GN  RK++FN RY+GK  E GGP FEE  H  A FT+LK++ 
Sbjct: 184 VNDGPGLVNIRIAAQHYGGNVFRKLIFNSRYFGKVMEDGGPGFEEVEHINATFTILKYVY 243

Query: 186 AFSILDFMPCLKPFDLGGHEK--------------------------------------- 245
           AFSI DF+P L+  DL GH                                         
Sbjct: 244 AFSISDFVPFLRRLDLDGHRSKIMKAMRIMRKYHDPIIDDRIKQWNDGLKTVEEDLLDVL 303

Query: 246 ---------------------IELQIATIDNPSNTVECAMAELLNQPKILQKDVEELDQV 305
                                IEL I  +DNPSN  E A+AE++NQP++L++  EELD V
Sbjct: 304 IKLKDANNKPLLTLKELKAQIIELAIEMVDNPSNAFEWALAEMINQPELLKRATEELDNV 363

Query: 306 VGRNRLVQESDIPNLKYLIACTRESFRLHPFSPFNVPHVSNCDIVVAGYFVPKGSQVLLS 348
           VG+ RLVQESDIP L+++ AC RE+ RLHP   FNVPH+   D +V  Y  PKG+QVLLS
Sbjct: 364 VGKERLVQESDIPKLQFVKACAREALRLHPMEYFNVPHLCMNDTMVGDYLFPKGTQVLLS 423

BLAST of CaUC03G061730 vs. ExPASy Swiss-Prot
Match: Q6J540 (Isoleucine N-monooxygenase 2 OS=Lotus japonicus OX=34305 GN=CYP79D4 PE=1 SV=1)

HSP 1 Score: 347.1 bits (889), Expect = 2.4e-94
Identity = 180/407 (44.23%), Postives = 243/407 (59.71%), Query Frame = 0

Query: 6   NSPTHQWIHAIMKQVNTEIACIRLGSNTHIIPVTSPELALEFLKTHDSVFVPRPISMTAE 65
           N P   WIH +MK++NTEIACIRL +NT +IPVT P +A EFLK HD+ F  RP  M+ +
Sbjct: 64  NRPATIWIHKLMKEMNTEIACIRL-ANTIVIPVTCPTIACEFLKKHDASFASRPKIMSTD 123

Query: 66  ILSRGYLSAVLSPTGDQWKKMRRILVSQVTNPPTLHRMLGRRTKKPDTLLHYIFS----- 125
           I S G+L+ VL P G+QWKKM+R+LV+ + +P     +LG+R ++ D L+ YI++     
Sbjct: 124 IASDGFLTTVLVPYGEQWKKMKRVLVNNLLSPQKHQWLLGKRNEEADNLMFYIYNKCCKD 183

Query: 126 LTRNSEAINIRSITQHYCGNTIRKMVFNGRYYGKGREHGGPTFEEENHNQALFTVLKHLN 185
           +      +NIR   QHY GN  RK++FN RY+GK  E GGP FEE  H  A FT+LK++ 
Sbjct: 184 VNDGPGLVNIRIAAQHYGGNVFRKLIFNTRYFGKVMEDGGPGFEEVEHINATFTILKYVY 243

Query: 186 AFSILDFMPCLKPFDLGGHEK--------------------------------------- 245
           AFSI DF+P L+  DL GH                                         
Sbjct: 244 AFSISDFIPFLRRLDLDGHRSKIMKAMGIMKKYHDPIIHDRIKQWNDGLKTVEEDLLDVL 303

Query: 246 ---------------------IELQIATIDNPSNTVECAMAELLNQPKILQKDVEELDQV 305
                                 EL I  +DNPSN  E A+AE+LNQP++L++  EELD V
Sbjct: 304 IKLKDASNKPLLTLKEIKAQITELAIEMVDNPSNAFEWALAEMLNQPELLKRATEELDNV 363

Query: 306 VGRNRLVQESDIPNLKYLIACTRESFRLHPFSPFNVPHVSNCDIVVAGYFVPKGSQVLLS 348
           VG+ RLVQESDIP L+++ AC RE+ RLHP   FNVPH+   D +V  Y  PKG+QVLLS
Sbjct: 364 VGKERLVQESDIPKLQFVKACAREALRLHPMEYFNVPHLCMNDTMVGDYLFPKGTQVLLS 423

BLAST of CaUC03G061730 vs. ExPASy Swiss-Prot
Match: Q43135 (Tyrosine N-monooxygenase OS=Sorghum bicolor OX=4558 GN=CYP79A1 PE=1 SV=3)

HSP 1 Score: 341.3 bits (874), Expect = 1.3e-92
Identity = 168/406 (41.38%), Postives = 242/406 (59.61%), Query Frame = 0

Query: 6   NSPTHQWIHAIMKQVNTEIACIRLGSNTHIIPVTSPELALEFLKTHDSVFVPRPISMTAE 65
           N P  +WIH +M+++ T+IAC++LG   H++ +T PE+A E L+  D+ F+ RP++  +E
Sbjct: 87  NKPAFRWIHQMMREMGTDIACVKLG-GVHVVSITCPEIAREVLRKQDANFISRPLTFASE 146

Query: 66  ILSRGYLSAVLSPTGDQWKKMRRILVSQVTNPPTLHRMLGRRTKKPDTLLHYIFSLTRNS 125
             S GY +AVLSP GDQWKKMRR+L S++  P     +  +RT + D L  Y+++L   +
Sbjct: 147 TFSGGYRNAVLSPYGDQWKKMRRVLTSEIICPSRHAWLHDKRTDEADNLTRYVYNLATKA 206

Query: 126 E----AINIRSITQHYCGNTIRKMVFNGRYYGKGREHGGPTFEEENHNQALFTVLKHLNA 185
                A+++R + +HYCGN IR+++FN RY+G+ +  GGP   E  H  A+FT L  L A
Sbjct: 207 ATGDVAVDVRHVARHYCGNVIRRLMFNRRYFGEPQADGGPGPMEVLHMDAVFTSLGLLYA 266

Query: 186 FSILDFMPCLKPFDLGGHEKI--------------------------------------- 245
           F + D++P L+  DL GHEKI                                       
Sbjct: 267 FCVSDYLPWLRGLDLDGHEKIVKEANVAVNRLHDTVIDDRWRQWKSGERQEMEDFLDVLI 326

Query: 246 ---------------------ELQIATIDNPSNTVECAMAELLNQPKILQKDVEELDQVV 305
                                ++  A +DNPSN VE A+AE++N P+++ K +EELD+VV
Sbjct: 327 TLKDAQGNPLLTIEEVKAQSQDITFAAVDNPSNAVEWALAEMVNNPEVMAKAMEELDRVV 386

Query: 306 GRNRLVQESDIPNLKYLIACTRESFRLHPFSPFNVPHVSNCDIVVAGYFVPKGSQVLLSR 348
           GR RLVQESDIP L Y+ AC RE+FRLHP +PFNVPHV+  D  +AGY VPKGS V+LSR
Sbjct: 387 GRERLVQESDIPKLNYVKACIREAFRLHPVAPFNVPHVALADTTIAGYRVPKGSHVILSR 446

BLAST of CaUC03G061730 vs. ExPASy TrEMBL
Match: A0A5A7VKT9 (Isoleucine N-monooxygenase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold14G00680 PE=3 SV=1)

HSP 1 Score: 540.0 bits (1390), Expect = 7.1e-150
Identity = 267/407 (65.60%), Postives = 306/407 (75.18%), Query Frame = 0

Query: 1   MLRNKNSPTHQWIHAIMKQVNTEIACIRLGSNTHIIPVTSPELALEFLKTHDSVFVPRPI 60
           MLRNKN PT+QWIH +MKQ NTEIACIRLGSNTHIIPV SPEL+LEFL THDSVF  R I
Sbjct: 51  MLRNKNLPTYQWIHEVMKQFNTEIACIRLGSNTHIIPVASPELSLEFLNTHDSVFGSRSI 110

Query: 61  SMTAEILSRGYLSAVLSPTGDQWKKMRRILVSQVTNPPTLHRMLGRRTKKPDTLLHYIFS 120
           SMTAEI+S GYLS VLSP G+QWKKMR+ILVSQV NPPTLH+M+G+RT + D LL YIF 
Sbjct: 111 SMTAEIVSNGYLSTVLSPMGEQWKKMRKILVSQVLNPPTLHQMVGQRTDEADILLRYIFG 170

Query: 121 LTRNSEAINIRSITQHYCGNTIRKMVFNGRYYGKGREHGGPTFEEENHNQALFTVLKHLN 180
           LTRN EAINIRSI +HYCG  IR+M+F+ RYYGKGRE GGP+ EEE+HNQAL ++L+H+N
Sbjct: 171 LTRNGEAINIRSIVRHYCGTVIRRMIFSRRYYGKGREDGGPSLEEEDHNQALLSILRHVN 230

Query: 181 AFSILDFMPCLKPFDLGGHEKI-------------------------------------- 240
           AFSI +F+P LK FDL GHEKI                                      
Sbjct: 231 AFSISEFIPLLKKFDLDGHEKIVKRALKVIRNNDEPIIEERVQDWRDGKKKKAEDILDIL 290

Query: 241 ----------------------ELQIATIDNPSNTVECAMAELLNQPKILQKDVEELDQV 300
                                 +LQ+A+IDNPSN VE A+AELLNQPKI+QK VEELD+V
Sbjct: 291 ISLKNENGKSLLSIEEIKAQVTDLQLASIDNPSNAVEWALAELLNQPKIIQKAVEELDKV 350

Query: 301 VGRNRLVQESDIPNLKYLIACTRESFRLHPFSPFNVPHVSNCDIVVAGYFVPKGSQVLLS 348
           +GR+RLVQE DIPNL YL AC RESFRLHPFSPFNVPHVSN D+ VAGYF+P+GSQVLLS
Sbjct: 351 IGRDRLVQECDIPNLMYLTACIRESFRLHPFSPFNVPHVSNSDVFVAGYFIPRGSQVLLS 410

BLAST of CaUC03G061730 vs. ExPASy TrEMBL
Match: A0A1S3BNA6 (phenylalanine N-monooxygenase-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103491937 PE=3 SV=1)

HSP 1 Score: 507.7 bits (1306), Expect = 3.9e-140
Identity = 254/403 (63.03%), Postives = 289/403 (71.71%), Query Frame = 0

Query: 5   KNSPTHQWIHAIMKQVNTEIACIRLGSNTHIIPVTSPELALEFLKTHDSVFVPRPISMTA 64
           K SP HQWIH +MKQ+NTEIAC  LGSNTHII VTSPELALEFLKTHDS F  RPI+ T 
Sbjct: 77  KRSPRHQWIHTVMKQLNTEIACFHLGSNTHIIIVTSPELALEFLKTHDSTFASRPIASTT 136

Query: 65  EILSRGYLSAVLSPTGDQWKKMRRILVSQVTNPPTLHRMLGRRTKKPDTLLHYIFSLTRN 124
           EILS G+LS VLSP GDQWKKM+RIL S V NP TLH+M G+R K+ D LLHYIF     
Sbjct: 137 EILSCGFLSTVLSPMGDQWKKMKRILASHVLNPSTLHQMRGQRIKEADALLHYIFDHASR 196

Query: 125 SEAINIRSITQHYCGNTIRKMVFNGRYYGKGREHGGPTFEEENHNQALFTVLKHLNAFSI 184
            EAINIRSITQHYC N IR+M+FN RYY KGRE+ GP+ EEE++NQAL T+L HL +FSI
Sbjct: 197 CEAINIRSITQHYCSNIIRRMIFNRRYYKKGRENEGPSLEEEDYNQALLTILMHLYSFSI 256

Query: 185 LDFMPCLKPFDLGGHEKI------------------------------------------ 244
            +++ CLKPFDLGGH+KI                                          
Sbjct: 257 SEYIACLKPFDLGGHQKIIRKALKVITNHDGPIIEERVQEWRDGKKKEVQDILDILITVK 316

Query: 245 ------------------ELQIATIDNPSNTVECAMAELLNQPKILQKDVEELDQVVGRN 304
                             ELQ+ATIDNPSN VE AM ELLNQPKILQK VEELD+VVGR+
Sbjct: 317 KENGESLLSIEEIKAQVTELQLATIDNPSNAVEWAMIELLNQPKILQKVVEELDEVVGRD 376

Query: 305 RLVQESDIPNLKYLIACTRESFRLHPFSPFNVPHVSNCDIVVAGYFVPKGSQVLLSRLGF 348
           RLVQE DIPNLKYL AC RESFRLHPFSPFN+PHVS  DIVV GYF+PKGSQ+LL R+G 
Sbjct: 377 RLVQEFDIPNLKYLTACMRESFRLHPFSPFNLPHVSISDIVVGGYFIPKGSQILLCRVGL 436

BLAST of CaUC03G061730 vs. ExPASy TrEMBL
Match: A0A1S3BPW4 (phenylalanine N-monooxygenase-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103491937 PE=3 SV=1)

HSP 1 Score: 507.7 bits (1306), Expect = 3.9e-140
Identity = 254/403 (63.03%), Postives = 289/403 (71.71%), Query Frame = 0

Query: 5   KNSPTHQWIHAIMKQVNTEIACIRLGSNTHIIPVTSPELALEFLKTHDSVFVPRPISMTA 64
           K SP HQWIH +MKQ+NTEIAC  LGSNTHII VTSPELALEFLKTHDS F  RPI+ T 
Sbjct: 77  KRSPRHQWIHTVMKQLNTEIACFHLGSNTHIIIVTSPELALEFLKTHDSTFASRPIASTT 136

Query: 65  EILSRGYLSAVLSPTGDQWKKMRRILVSQVTNPPTLHRMLGRRTKKPDTLLHYIFSLTRN 124
           EILS G+LS VLSP GDQWKKM+RIL S V NP TLH+M G+R K+ D LLHYIF     
Sbjct: 137 EILSCGFLSTVLSPMGDQWKKMKRILASHVLNPSTLHQMRGQRIKEADALLHYIFDHASR 196

Query: 125 SEAINIRSITQHYCGNTIRKMVFNGRYYGKGREHGGPTFEEENHNQALFTVLKHLNAFSI 184
            EAINIRSITQHYC N IR+M+FN RYY KGRE+ GP+ EEE++NQAL T+L HL +FSI
Sbjct: 197 CEAINIRSITQHYCSNIIRRMIFNRRYYKKGRENEGPSLEEEDYNQALLTILMHLYSFSI 256

Query: 185 LDFMPCLKPFDLGGHEKI------------------------------------------ 244
            +++ CLKPFDLGGH+KI                                          
Sbjct: 257 SEYIACLKPFDLGGHQKIIRKALKVITNHDGPIIEERVQEWRDGKKKEVQDILDILITVK 316

Query: 245 ------------------ELQIATIDNPSNTVECAMAELLNQPKILQKDVEELDQVVGRN 304
                             ELQ+ATIDNPSN VE AM ELLNQPKILQK VEELD+VVGR+
Sbjct: 317 KENGESLLSIEEIKAQVTELQLATIDNPSNAVEWAMIELLNQPKILQKVVEELDEVVGRD 376

Query: 305 RLVQESDIPNLKYLIACTRESFRLHPFSPFNVPHVSNCDIVVAGYFVPKGSQVLLSRLGF 348
           RLVQE DIPNLKYL AC RESFRLHPFSPFN+PHVS  DIVV GYF+PKGSQ+LL R+G 
Sbjct: 377 RLVQEFDIPNLKYLTACMRESFRLHPFSPFNLPHVSISDIVVGGYFIPKGSQILLCRVGL 436

BLAST of CaUC03G061730 vs. ExPASy TrEMBL
Match: A0A6J1D6G9 (isoleucine N-monooxygenase 2-like OS=Momordica charantia OX=3673 GN=LOC111017498 PE=3 SV=1)

HSP 1 Score: 505.8 bits (1301), Expect = 1.5e-139
Identity = 258/414 (62.32%), Postives = 297/414 (71.74%), Query Frame = 0

Query: 1   MLRNKNSPTHQWIHAIMKQVNTEIACIRLGSNTHIIPVTSPELALEFLKTHDSVFVPR-P 60
           ML  K SP ++WIHA+MKQ +TEIACIRLG NT+++PVTSPELA+EFLK HDSVF  R  
Sbjct: 66  MLATK-SPKYKWIHAVMKQFDTEIACIRLG-NTYVMPVTSPELAVEFLKRHDSVFASRST 125

Query: 61  ISMTAEILSRGYLSAVLSPTGDQWKKMRRILVSQVTNPPTLHRMLGRRTKKPDTLLHYIF 120
           IS T  +LSRG L+   SP G QWKKMRRIL S++  P TLH+MLG RT + DTLL YIF
Sbjct: 126 ISNTVNVLSRGLLTTAFSPLGHQWKKMRRILASEIFCPSTLHQMLGHRTNEADTLLRYIF 185

Query: 121 SLT------RNSEAINIRSITQHYCGNTIRKMVFNGRYYGKGREHGGPTFEEENHNQALF 180
           SLT      ++   +N+RS+TQHYCGN IR+M+FN RYYGKGRE GGP+FEEE HNQAL 
Sbjct: 186 SLTTTNTVNKDGAVVNVRSVTQHYCGNIIRRMLFNKRYYGKGREDGGPSFEEEEHNQALL 245

Query: 181 TVLKHLNAFSILDFMPCLKPFDLGGHEKI------------------------------- 240
           T+L+H+NAFSI DFMPCLKPFDL GHEKI                               
Sbjct: 246 TILRHVNAFSISDFMPCLKPFDLDGHEKIMKRALKALWKYDEPIINERVQQWKDGKREGA 305

Query: 241 -----------------------------ELQIATIDNPSNTVECAMAELLNQPKILQKD 300
                                        ELQIATIDNPSN VE  MAELLNQPKILQK 
Sbjct: 306 EDILDILISTKDGNGKSLLTIDEIKAQITELQIATIDNPSNAVEWTMAELLNQPKILQKA 365

Query: 301 VEELDQVVGRNRLVQESDIPNLKYLIACTRESFRLHPFSPFNVPHVSNCDIVVAGYFVPK 348
           +EELD+VVGR RLVQESDIP LKYL AC RESFRLHPFSPFN+PHVSN DIVVAGYF+PK
Sbjct: 366 IEELDRVVGRERLVQESDIPKLKYLTACARESFRLHPFSPFNIPHVSNADIVVAGYFIPK 425

BLAST of CaUC03G061730 vs. ExPASy TrEMBL
Match: A0A6J1D470 (isoleucine N-monooxygenase 2-like OS=Momordica charantia OX=3673 GN=LOC111017448 PE=3 SV=1)

HSP 1 Score: 500.4 bits (1287), Expect = 6.2e-138
Identity = 249/408 (61.03%), Postives = 293/408 (71.81%), Query Frame = 0

Query: 6   NSPTHQWIHAIMKQVNTEIACIRLGSNTHIIPVTSPELALEFLKTHDSVFVPRPISMTAE 65
           NSP +QWIHA+MKQ N+EIACIRLG NT+IIPVTSPELALEFLKT+DS F  RP+  T  
Sbjct: 58  NSPKYQWIHAVMKQFNSEIACIRLG-NTYIIPVTSPELALEFLKTYDSAFSSRPVHQTIN 117

Query: 66  ILSRGYLSAVLSPTGDQWKKMRRILVSQVTNPPTLHRMLGRRTKKPDTLLHYIFSLT--- 125
           IL+ G+L  + SP GDQWKKMRRIL SQ+ +  TL RM+G RT + DTLL YIFSLT   
Sbjct: 118 ILTNGFLGTIFSPMGDQWKKMRRILASQIFSSSTLQRMIGHRTDEADTLLRYIFSLTTTN 177

Query: 126 ---RNSEAINIRSITQHYCGNTIRKMVFNGRYYGKGREHGGPTFEEENHNQALFTVLKHL 185
              + +  +N+RS+TQHY GN IR+M+FN RYYGKGRE GGPT EEE HNQAL T+L+H+
Sbjct: 178 TSNKGNAVVNVRSVTQHYSGNIIRRMLFNRRYYGKGREDGGPTTEEEEHNQALLTILRHV 237

Query: 186 NAFSILDFMPCLKPFDLGGHEK-------------------------------------- 245
           NAFSILDFMPCLKPFDL GHEK                                      
Sbjct: 238 NAFSILDFMPCLKPFDLDGHEKTMKKALEVLRKYDEPIINKRVHQWKDGKREGVEDILDI 297

Query: 246 ----------------------IELQIATIDNPSNTVECAMAELLNQPKILQKDVEELDQ 305
                                 +ELQ+A IDNPSN VE  +AELLNQ KILQK +EE+D+
Sbjct: 298 LISLQDENKRMLLSIEEIKAQTMELQLAMIDNPSNAVEWTIAELLNQSKILQKVIEEMDR 357

Query: 306 VVGRNRLVQESDIPNLKYLIACTRESFRLHPFSPFNVPHVSNCDIVVAGYFVPKGSQVLL 348
           +VGR+RLVQESDIP LKYL AC RESFRLHPFSPFNVPHVSN DI+VAGYF+PKGS+V+L
Sbjct: 358 IVGRDRLVQESDIPKLKYLTACVRESFRLHPFSPFNVPHVSNSDIIVAGYFIPKGSEVVL 417

BLAST of CaUC03G061730 vs. TAIR 10
Match: AT5G05260.1 (cytochrome p450 79a2 )

HSP 1 Score: 357.1 bits (915), Expect = 1.6e-98
Identity = 181/409 (44.25%), Postives = 251/409 (61.37%), Query Frame = 0

Query: 5   KNSPTHQWIHAIMKQVNTEIACIRLGSNTHIIPVTSPELALEFLKTHDSVFVPRPISMTA 64
           +N P  +WIH++MK++NT+IACIRL +NTH+IPVTSP +A E LK  DSVF  RP++M  
Sbjct: 53  RNKPVFRWIHSLMKELNTDIACIRL-ANTHVIPVTSPRIAREILKKQDSVFATRPLTMGT 112

Query: 65  EILSRGYLSAVLSPTGDQWKKMRRILVSQVTNPPTLHRMLGRRTKKPDTLLHYIFSLTRN 124
           E  SRGYL+  + P G+QWKKMRR++ S VT+  +   ML +RT++ D L+ YI + +  
Sbjct: 113 EYCSRGYLTVAVEPQGEQWKKMRRVVASHVTSKKSFQMMLQKRTEEADNLVRYINNRSVK 172

Query: 125 SE-----AINIRSITQHYCGNTIRKMVFNGRYYGKGREHG-GPTFEEENHNQALFTVLKH 184
           +       I++R   + Y GN  RKM+F  R++GKG E G GP  EE  H ++LFTVL H
Sbjct: 173 NRGNAFVVIDLRLAVRQYSGNVARKMMFGIRHFGKGSEDGSGPGLEEIEHVESLFTVLTH 232

Query: 185 LNAFSILDFMPCLKPFDLGGHEKI------------------------------------ 244
           L AF++ D++P L+  DL GHEK+                                    
Sbjct: 233 LYAFALSDYVPWLRFLDLEGHEKVVSNAMRNVSKYNDPFVDERLMQWRNGKMKEPQDFLD 292

Query: 245 ------------------------ELQIATIDNPSNTVECAMAELLNQPKILQKDVEELD 304
                                   EL +AT+DNPSN  E  MAE++N+P I+QK VEE+D
Sbjct: 293 MFIIAKDTDGKPTLSDEEIKAQVTELMLATVDNPSNAAEWGMAEMINEPSIMQKAVEEID 352

Query: 305 QVVGRNRLVQESDIPNLKYLIACTRESFRLHPFSPFNVPHVSNCDIVVAGYFVPKGSQVL 348
           +VVG++RLV ESD+PNL Y+ AC +E+FRLHP +PFN+PH+S  D VV GYF+PKGS VL
Sbjct: 353 RVVGKDRLVIESDLPNLNYVKACVKEAFRLHPVAPFNLPHMSTTDTVVDGYFIPKGSHVL 412

BLAST of CaUC03G061730 vs. TAIR 10
Match: AT2G22330.1 (cytochrome P450, family 79, subfamily B, polypeptide 3 )

HSP 1 Score: 330.5 bits (846), Expect = 1.6e-90
Identity = 166/404 (41.09%), Postives = 245/404 (60.64%), Query Frame = 0

Query: 5   KNSPTHQWIHAIMKQVNTEIACIRLGSNTHIIPVTSPELALEFLKTHDSVFVPRPISMTA 64
           KN P  +W+H++MK++NTEIAC+RLG NTH+IPVT P++A E  K  D++F  RP++   
Sbjct: 76  KNRPVFRWLHSLMKELNTEIACVRLG-NTHVIPVTCPKIAREIFKQQDALFASRPLTYAQ 135

Query: 65  EILSRGYLSAVLSPTGDQWKKMRRILVSQVTNPPTLHRMLGRRTKKPDTLLHYIFSLTRN 124
           +ILS GY + V++P G+Q+KKMR+++++++  P     +   R ++ D L  +++++ +N
Sbjct: 136 KILSNGYKTCVITPFGEQFKKMRKVIMTEIVCPARHRWLHDNRAEETDHLTAWLYNMVKN 195

Query: 125 SEAINIRSITQHYCGNTIRKMVFNGRYYGKGRE-HGGPTFEEENHNQALFTVLKHLNAFS 184
           SE +++R +T+HYCGN I++++F  R + +  E  GGPT E+  H  A+F  L    AF 
Sbjct: 196 SEPVDLRFVTRHYCGNAIKRLMFGTRTFSEKTEADGGPTLEDIEHMDAMFEGLGFTFAFC 255

Query: 185 ILDFMPCLKPFDLGGHEKI----------------------------------------- 244
           I D++P L   DL GHEKI                                         
Sbjct: 256 ISDYLPMLTGLDLNGHEKIMRESSAIMDKYHDPIIDERIKMWREGKRTQIEDFLDIFISI 315

Query: 245 -------------------ELQIATIDNPSNTVECAMAELLNQPKILQKDVEELDQVVGR 304
                              EL +A  DNPSN VE A+AE++N+P+IL K +EE+D+VVG+
Sbjct: 316 KDEAGQPLLTADEIKPTIKELVMAAPDNPSNAVEWAIAEMINKPEILHKAMEEIDRVVGK 375

Query: 305 NRLVQESDIPNLKYLIACTRESFRLHPFSPFNVPHVSNCDIVVAGYFVPKGSQVLLSRLG 348
            R VQESDIP L Y+ A  RE+FRLHP + FN+PHV+  D  VAGY +PKGSQVLLSR G
Sbjct: 376 ERFVQESDIPKLNYVKAIIREAFRLHPVAAFNLPHVALSDTTVAGYHIPKGSQVLLSRYG 435

BLAST of CaUC03G061730 vs. TAIR 10
Match: AT4G39950.1 (cytochrome P450, family 79, subfamily B, polypeptide 2 )

HSP 1 Score: 330.5 bits (846), Expect = 1.6e-90
Identity = 169/404 (41.83%), Postives = 248/404 (61.39%), Query Frame = 0

Query: 5   KNSPTHQWIHAIMKQVNTEIACIRLGSNTHIIPVTSPELALEFLKTHDSVFVPRPISMTA 64
           K+ P  +W+H+IMKQ+NTEIAC++LG NTH+I VT P++A E LK  D++F  RP++   
Sbjct: 74  KSRPVFRWLHSIMKQLNTEIACVKLG-NTHVITVTCPKIAREILKQQDALFASRPLTYAQ 133

Query: 65  EILSRGYLSAVLSPTGDQWKKMRRILVSQVTNPPTLHRMLGRRTKKPDTLLHYIFSLTRN 124
           +ILS GY + V++P GDQ+KKMR+++++++  P     +  +R+++ D L  +++++ +N
Sbjct: 134 KILSNGYKTCVITPFGDQFKKMRKVVMTELVCPARHRWLHQKRSEENDHLTAWVYNMVKN 193

Query: 125 SEAINIRSITQHYCGNTIRKMVFNGRYYGKG-REHGGPTFEEENHNQALFTVLKHLNAFS 184
           S +++ R +T+HYCGN I+K++F  R + K     GGPT E+  H +A+F  L    AF 
Sbjct: 194 SGSVDFRFMTRHYCGNAIKKLMFGTRTFSKNTAPDGGPTVEDVEHMEAMFEALGFTFAFC 253

Query: 185 ILDFMPCLKPFDLGGHEKI----------------------------------------- 244
           I D++P L   DL GHEKI                                         
Sbjct: 254 ISDYLPMLTGLDLNGHEKIMRESSAIMDKYHDPIIDERIKMWREGKRTQIEDFLDIFISI 313

Query: 245 -------------------ELQIATIDNPSNTVECAMAELLNQPKILQKDVEELDQVVGR 304
                              EL +A  DNPSN VE AMAE++N+P+IL+K +EE+D+VVG+
Sbjct: 314 KDEQGNPLLTADEIKPTIKELVMAAPDNPSNAVEWAMAEMVNKPEILRKAMEEIDRVVGK 373

Query: 305 NRLVQESDIPNLKYLIACTRESFRLHPFSPFNVPHVSNCDIVVAGYFVPKGSQVLLSRLG 348
            RLVQESDIP L Y+ A  RE+FRLHP + FN+PHV+  D  VAGY +PKGSQVLLSR G
Sbjct: 374 ERLVQESDIPKLNYVKAILREAFRLHPVAAFNLPHVALSDTTVAGYHIPKGSQVLLSRYG 433

BLAST of CaUC03G061730 vs. TAIR 10
Match: AT5G35917.1 (cytochrome P450, family 79, subfamily A, polypeptide 3 pseudogene )

HSP 1 Score: 295.4 bits (755), Expect = 5.8e-80
Identity = 157/391 (40.15%), Postives = 224/391 (57.29%), Query Frame = 0

Query: 5   KNSPTHQWIHAIMKQVNTEIACIRLGSNTHIIPVTSPELALEFLKTHDSVFVPRPISMTA 64
           +N P  +WIH++M+++NT+IACIRL +NTH+IPVTSP +A E LK  DSVF  RP++M  
Sbjct: 61  RNKPVFRWIHSLMEELNTDIACIRL-ANTHVIPVTSPRIAREILKKQDSVFAIRPLTMGT 120

Query: 65  EILSRGYLSAVLSPTGDQWKKMRRILVSQVTNPPTLHRMLGRRTKKPDTLLHYIFSLTRN 124
           E  SRGYL+  +   G+QWKKMRR++ S VT+  +   ML +RT++ D L+ YI +    
Sbjct: 121 EYCSRGYLTIAVQSQGEQWKKMRRVVASHVTSKKSFKLMLEKRTEEADNLVRYINNRCVK 180

Query: 125 SE-------AINIRSITQHYCGNTIRKMVFNGRYYGKGRE-HGGPTFEEENHNQALFTVL 184
           +         I++R + + Y GN  RKM+F  R++GKG E   GP  EE  H ++LFTVL
Sbjct: 181 NRGNGNGLAVIDLRFVVRQYSGNVARKMMFGIRHFGKGSEDESGPGSEEIEHVESLFTVL 240

Query: 185 KHLNAFSILDFMPCLKPFDLGGHEKI---------------------------------- 244
            HL AF++ D++P L+  DL GHEK+                                  
Sbjct: 241 THLYAFALSDYVPWLRFLDLEGHEKVVADAMRNVSKYNDPFVDERLMQWRNGKMKEPQDF 300

Query: 245 --------------------------ELQIATIDNPSNTVECAMAELLNQPKILQKDVEE 304
                                     EL +AT+DNPSN  E AMAE++N+P I+QK VEE
Sbjct: 301 LDMFIMAKDTNGKPTLSEEEIKAQVTELMLATVDNPSNAAEWAMAEMINEPSIMQKAVEE 360

Query: 305 LDQVVGRNRLVQESDIPNLKYLIACTRESFRLHPFSPFNVPHVSNCDIVVAGYFVPKGSQ 328
           +D+              NL+  +    E+FRLHP +PFN+PH+S  D VV GYF+PKGS 
Sbjct: 361 IDR-------------SNLRESVC--EETFRLHPVAPFNLPHMSTADAVVDGYFIPKGSH 420

BLAST of CaUC03G061730 vs. TAIR 10
Match: AT1G58260.1 (cytochrome p450 79c2 )

HSP 1 Score: 254.2 bits (648), Expect = 1.5e-67
Identity = 142/410 (34.63%), Postives = 226/410 (55.12%), Query Frame = 0

Query: 6   NSPTHQWIHAIMKQVNTEIACIRLGSNTHIIPVTSPELALEFLKTHDSVFVPRPISMTAE 65
           N P H WIH +M+++ T+IAC R  +  H+I VTS ++A E L+  D V   R  S  + 
Sbjct: 56  NRPAHLWIHRVMEELQTDIACYRF-ARFHVITVTSSKIAREVLREKDEVLADRSESYASH 115

Query: 66  ILSRGYLSAVLSPTGDQWKKMRRILVSQVTNPPTLHRMLGRRTKKPDTLLHYIFSLTRNS 125
           ++S GY +   S  G+ WK +++++ +++ +P TL + LG R  + D ++ Y+++L +  
Sbjct: 116 LISHGYKNISFSSYGENWKLVKKVMTTKLMSPTTLSKTLGYRNIEADNIVTYVYNLCQLG 175

Query: 126 ---EAINIRSITQHYCGNTIRKMVFNGRYYGKGREHGGPTFEEENHNQALFTVLKHLNAF 185
              + IN+R     YC   + +M+F  R++ +  E+GG   +E+ H  A++  L    +F
Sbjct: 176 SVRKPINVRDTILTYCHAVMMRMMFGQRHFDEVVENGGLGPKEKEHMDAIYLALDCFFSF 235

Query: 186 SILDFMPCLKPF------------------------------------------------ 245
           ++ +++P L+ +                                                
Sbjct: 236 NLTNYIPFLRGWNVDKAETEVREAVHIINICNDPIIQERIHLWRKKGGKQMEEDWLDILI 295

Query: 246 ----DLGGH---------EKIELQIATIDNPSNTVECAMAELLNQPKILQKDVEELDQVV 305
               D G H         +  E+ +ATIDN  N VE  +AE+LN P+IL+K   ELD +V
Sbjct: 296 TLKDDQGMHLFTFDEIRAQCKEINLATIDNTMNNVEWTIAEMLNHPEILEKATNELDIIV 355

Query: 306 GRNRLVQESDIPNLKYLIACTRESFRLHPFSPFNVPHVSNCDIVVAGYFVPKGSQVLLSR 348
           G++RLVQESDI  L Y+ AC++ESFRLHP + F   HV+  D  +AGYFVPKGSQ+L+SR
Sbjct: 356 GKDRLVQESDISQLNYIKACSKESFRLHPANVFMPHHVAREDTTLAGYFVPKGSQILVSR 415

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAA0067056.11.5e-14965.60isoleucine N-monooxygenase 1 [Cucumis melo var. makuwa] >TYK26293.1 isoleucine N... [more]
XP_011655052.17.5e-14664.37isoleucine N-monooxygenase 1 [Cucumis sativus] >KAE8648059.1 hypothetical protei... [more]
XP_038880438.19.5e-14163.99tryptophan N-monooxygenase CYP79A68-like [Benincasa hispida][more]
XP_008450287.18.0e-14063.03PREDICTED: phenylalanine N-monooxygenase-like isoform X1 [Cucumis melo][more]
XP_008450288.18.0e-14063.03PREDICTED: phenylalanine N-monooxygenase-like isoform X2 [Cucumis melo][more]
Match NameE-valueIdentityDescription
A0A068Q6051.5e-10144.47Tryptophan N-monooxygenase CYP79A68 OS=Prunus mume OX=102107 GN=CYP79A68 PE=1 SV... [more]
Q9FLC82.3e-9744.25Phenylalanine N-monooxygenase OS=Arabidopsis thaliana OX=3702 GN=CYP79A2 PE=1 SV... [more]
Q6J5411.1e-9443.98Isoleucine N-monooxygenase 1 OS=Lotus japonicus OX=34305 GN=CYP79D3 PE=1 SV=1[more]
Q6J5402.4e-9444.23Isoleucine N-monooxygenase 2 OS=Lotus japonicus OX=34305 GN=CYP79D4 PE=1 SV=1[more]
Q431351.3e-9241.38Tyrosine N-monooxygenase OS=Sorghum bicolor OX=4558 GN=CYP79A1 PE=1 SV=3[more]
Match NameE-valueIdentityDescription
A0A5A7VKT97.1e-15065.60Isoleucine N-monooxygenase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A1S3BNA63.9e-14063.03phenylalanine N-monooxygenase-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC1034... [more]
A0A1S3BPW43.9e-14063.03phenylalanine N-monooxygenase-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC1034... [more]
A0A6J1D6G91.5e-13962.32isoleucine N-monooxygenase 2-like OS=Momordica charantia OX=3673 GN=LOC111017498... [more]
A0A6J1D4706.2e-13861.03isoleucine N-monooxygenase 2-like OS=Momordica charantia OX=3673 GN=LOC111017448... [more]
Match NameE-valueIdentityDescription
AT5G05260.11.6e-9844.25cytochrome p450 79a2 [more]
AT2G22330.11.6e-9041.09cytochrome P450, family 79, subfamily B, polypeptide 3 [more]
AT4G39950.11.6e-9041.83cytochrome P450, family 79, subfamily B, polypeptide 2 [more]
AT5G35917.15.8e-8040.15cytochrome P450, family 79, subfamily A, polypeptide 3 pseudogene [more]
AT1G58260.11.5e-6734.63cytochrome p450 79c2 [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (USVL246-FR2) v1
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 339..347
score: 35.5
coord: 259..277
score: 33.82
coord: 300..324
score: 24.3
coord: 137..155
score: 22.94
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 1..206
e-value: 2.1E-20
score: 74.7
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 207..347
e-value: 7.6E-37
score: 128.9
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 6..347
IPR001128Cytochrome P450PFAMPF00067p450coord: 202..347
e-value: 6.5E-34
score: 117.5
coord: 22..189
e-value: 6.0E-9
score: 35.2
NoneNo IPR availablePANTHERPTHR24298:SF675PHENYLALANINE N-MONOOXYGENASEcoord: 5..202
coord: 202..347
NoneNo IPR availablePANTHERPTHR24298FLAVONOID 3'-MONOOXYGENASE-RELATEDcoord: 5..202
coord: 202..347

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CaUC03G061730.1CaUC03G061730.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016020 membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen