Homology
BLAST of CaUC03G061480 vs. NCBI nr
Match:
XP_038877759.1 (LOW QUALITY PROTEIN: beta-amyrin 28-monooxygenase-like [Benincasa hispida])
HSP 1 Score: 689.1 bits (1777), Expect = 2.8e-194
Identity = 348/443 (78.56%), Postives = 363/443 (81.94%), Query Frame = 0
Query: 57 MEHFLISLLILFLFLLSLTFFILFHKHKSLFSYPNVPPGAIGFPILGESLEFLSTGWKGH 116
MEHFLISLLI FLF LSLT FILFH HKSLFSYPNVPPGAIG PILGESLEFLSTGWKG
Sbjct: 1 MEHFLISLLIFFLFFLSLTLFILFHNHKSLFSYPNVPPGAIGLPILGESLEFLSTGWKGE 60
Query: 117 PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPS 176
PEKFIFDRL+KYKSDVFKTSIVGVPAAIFCG ACNKFLFSNENKLVT WWPDSVNKIFPS
Sbjct: 61 PEKFIFDRLHKYKSDVFKTSIVGVPAAIFCGSACNKFLFSNENKLVTPWWPDSVNKIFPS 120
Query: 177 STQTNSKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVFPLAKSL 236
STQT+SKEEAKKLKKFLPQFLKPEALQRYIG+MDELAQRHFASFWENREEVLVFPLAKS
Sbjct: 121 STQTSSKEEAKKLKKFLPQFLKPEALQRYIGVMDELAQRHFASFWENREEVLVFPLAKS- 180
Query: 237 IEIQFLQSLILVDRLPIMELVSCSRSISISASSQSRSISFSVLIFLKLISDEIRFTFSLA 296
FTFSLA
Sbjct: 181 ------------------------------------------------------FTFSLA 240
Query: 297 CRLFVSVDDEIQVERLSRPFEHIVAGIISLPIDLPGTPFNRAIKASNFIRKELVAIVRQR 356
CRLFVSV+DEI V+RLS PFEHI AGIIS+PIDLPGTPFNRAIKAS FIRKELVAIV++R
Sbjct: 241 CRLFVSVEDEIHVKRLSGPFEHIAAGIISVPIDLPGTPFNRAIKASKFIRKELVAIVKKR 300
Query: 357 KWDLAEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVACTFILKF 416
+WDLAEGKAS TQDILSHMLLTCDENG +MNESDIADKILGLLIGGHDT SVACTFI+KF
Sbjct: 301 RWDLAEGKASATQDILSHMLLTCDENGVFMNESDIADKILGLLIGGHDTASVACTFIVKF 360
Query: 417 LAELPHVYDGVYAEQMEIAKAKAPGEALKWEDINKMKYSWNVACEVLRIASPVQGAFREA 476
LAELPH+Y GVY EQMEIAK KAPGEALKWEDI KMKYSWNV CEVLR+ASP QGAFREA
Sbjct: 361 LAELPHIYHGVYTEQMEIAKGKAPGEALKWEDIKKMKYSWNVVCEVLRLASPXQGAFREA 388
Query: 477 LNDFVFNGFYIPKGWKVIFSPPS 500
L DFVFNGFYIPKGWK+ +S S
Sbjct: 421 LTDFVFNGFYIPKGWKLYWSANS 388
BLAST of CaUC03G061480 vs. NCBI nr
Match:
XP_008443638.1 (PREDICTED: beta-amyrin 28-oxidase [Cucumis melo])
HSP 1 Score: 676.0 bits (1743), Expect = 2.5e-190
Identity = 341/443 (76.98%), Postives = 359/443 (81.04%), Query Frame = 0
Query: 57 MEHFLISLLILFLFLLSLTFFILFHKHKSLFSYPNVPPGAIGFPILGESLEFLSTGWKGH 116
MEHFLISLLIL F LSLT FILFH HKSLFSYPN PPGAIG PILGESLEFLS+GW+GH
Sbjct: 1 MEHFLISLLILLFFFLSLTLFILFHNHKSLFSYPNTPPGAIGLPILGESLEFLSSGWRGH 60
Query: 117 PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPS 176
PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGP CNKFLFSNENKLVT WWPDSVNKIFPS
Sbjct: 61 PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNKIFPS 120
Query: 177 STQTNSKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVFPLAKSL 236
+TQT+SKEEAKKLKK LPQFLKPEALQRYIGIMDELA+RHF SFW+NREEVLVFPLAKS
Sbjct: 121 TTQTSSKEEAKKLKKLLPQFLKPEALQRYIGIMDELAERHFNSFWKNREEVLVFPLAKS- 180
Query: 237 IEIQFLQSLILVDRLPIMELVSCSRSISISASSQSRSISFSVLIFLKLISDEIRFTFSLA 296
FTFSLA
Sbjct: 181 ------------------------------------------------------FTFSLA 240
Query: 297 CRLFVSVDDEIQVERLSRPFEHIVAGIISLPIDLPGTPFNRAIKASNFIRKELVAIVRQR 356
CRLF+SV+DEI VERLS PFEHI AGIIS+PIDLPGTPFNRAIKAS FIRKE+VAIVRQR
Sbjct: 241 CRLFMSVEDEIHVERLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEVVAIVRQR 300
Query: 357 KWDLAEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVACTFILKF 416
K DLAEGKA TQDILSHMLLTCDENG YMNESDI DKILGLLIGGHDT SVACTFI+KF
Sbjct: 301 KQDLAEGKALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVACTFIVKF 360
Query: 417 LAELPHVYDGVYAEQMEIAKAKAPGEALKWEDINKMKYSWNVACEVLRIASPVQGAFREA 476
LAELPH+YD VY EQMEIA+AKA GE LKWEDI KMKYSWNVACEVLR+ASP+QGAFREA
Sbjct: 361 LAELPHIYDAVYTEQMEIARAKAEGETLKWEDIKKMKYSWNVACEVLRLASPLQGAFREA 388
Query: 477 LNDFVFNGFYIPKGWKVIFSPPS 500
LNDFVFNGF+IPKGWK+ +S S
Sbjct: 421 LNDFVFNGFFIPKGWKLYWSANS 388
BLAST of CaUC03G061480 vs. NCBI nr
Match:
XP_004139087.1 (beta-amyrin 28-monooxygenase [Cucumis sativus] >KGN66615.1 hypothetical protein Csa_007564 [Cucumis sativus])
HSP 1 Score: 668.7 bits (1724), Expect = 4.0e-188
Identity = 338/443 (76.30%), Postives = 358/443 (80.81%), Query Frame = 0
Query: 57 MEHFLISLLILFLFLLSLTFFILFHKHKSLFSYPNVPPGAIGFPILGESLEFLSTGWKGH 116
ME FLISLLIL F LSLT FILFH HKSLFSYPN PPGAIG PILGES+EFLS+GWKGH
Sbjct: 1 MELFLISLLILLFFFLSLTLFILFHNHKSLFSYPNTPPGAIGLPILGESVEFLSSGWKGH 60
Query: 117 PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPS 176
PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGP CNKFLFSNENKLVT WWPDSVNKIFPS
Sbjct: 61 PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNKIFPS 120
Query: 177 STQTNSKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVFPLAKSL 236
+TQT++KEEAKKLKK LPQFLKPEALQRYIGIMDELA+RHF SFW+NREEVLVFPLAKS
Sbjct: 121 TTQTSTKEEAKKLKKLLPQFLKPEALQRYIGIMDELAERHFNSFWKNREEVLVFPLAKS- 180
Query: 237 IEIQFLQSLILVDRLPIMELVSCSRSISISASSQSRSISFSVLIFLKLISDEIRFTFSLA 296
FTFS+A
Sbjct: 181 ------------------------------------------------------FTFSIA 240
Query: 297 CRLFVSVDDEIQVERLSRPFEHIVAGIISLPIDLPGTPFNRAIKASNFIRKELVAIVRQR 356
CRLF+SV+DEI VERLS PFEHI AGIIS+PIDLPGTPFNRAIKAS FIRKE+VAIVRQR
Sbjct: 241 CRLFMSVEDEIHVERLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEVVAIVRQR 300
Query: 357 KWDLAEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVACTFILKF 416
K DLAEGKA TQDILSHMLLTCDENG YMNESDI DKILGLLIGGHDT SVACTFI+KF
Sbjct: 301 KQDLAEGKALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVACTFIVKF 360
Query: 417 LAELPHVYDGVYAEQMEIAKAKAPGEALKWEDINKMKYSWNVACEVLRIASPVQGAFREA 476
LAELPH+YD VY EQMEIA+AKA GE LKWEDI KMKYSWNVACEVLRIASP+QGAFREA
Sbjct: 361 LAELPHIYDAVYTEQMEIARAKAEGETLKWEDIKKMKYSWNVACEVLRIASPLQGAFREA 388
Query: 477 LNDFVFNGFYIPKGWKVIFSPPS 500
L+DFVFNGF+IPKGWK+ +S S
Sbjct: 421 LSDFVFNGFFIPKGWKLYWSANS 388
BLAST of CaUC03G061480 vs. NCBI nr
Match:
KAG6590124.1 (Beta-amyrin 28-monooxygenase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7023791.1 hypothetical protein SDJN02_14817 [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 653.3 bits (1684), Expect = 1.7e-183
Identity = 330/443 (74.49%), Postives = 355/443 (80.14%), Query Frame = 0
Query: 57 MEHFLISLLILFLFLLSLTFFILFHKHKSLFSYPNVPPGAIGFPILGESLEFLSTGWKGH 116
M FLISLLILFL L+SLT F+LF+KHKSLFSYPN PPGAIG PI+GESLEFLSTGWKG
Sbjct: 1 MADFLISLLILFLSLISLTLFVLFYKHKSLFSYPNTPPGAIGLPIIGESLEFLSTGWKGE 60
Query: 117 PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPS 176
PEKFIFDRLNKYKSD+FKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFP+
Sbjct: 61 PEKFIFDRLNKYKSDIFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPA 120
Query: 177 STQTNSKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVFPLAKSL 236
ST+ NSKE A +LKK LP+FLKPEALQRYIG+MDELAQRHFAS WENRE+VLVFPLAKS
Sbjct: 121 STEINSKESAIRLKKLLPRFLKPEALQRYIGVMDELAQRHFASSWENREQVLVFPLAKS- 180
Query: 237 IEIQFLQSLILVDRLPIMELVSCSRSISISASSQSRSISFSVLIFLKLISDEIRFTFSLA 296
FTFSLA
Sbjct: 181 ------------------------------------------------------FTFSLA 240
Query: 297 CRLFVSVDDEIQVERLSRPFEHIVAGIISLPIDLPGTPFNRAIKASNFIRKELVAIVRQR 356
CRLFVS++D I VERLS PF+ I AGIIS+PIDLPGTPFNRAIKASNF+RKEL++IVRQR
Sbjct: 241 CRLFVSLEDRIHVERLSEPFQSIAAGIISMPIDLPGTPFNRAIKASNFVRKELLSIVRQR 300
Query: 357 KWDLAEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVACTFILKF 416
K DLAEGKASPTQDILSHMLLTCDE G YMNES IAD ILGLLIGGHDTTS ACTFI+K+
Sbjct: 301 KSDLAEGKASPTQDILSHMLLTCDEIGQYMNESAIADNILGLLIGGHDTTSAACTFIVKY 360
Query: 417 LAELPHVYDGVYAEQMEIAKAKAPGEALKWEDINKMKYSWNVACEVLRIASPVQGAFREA 476
LAELP +YDGVY EQMEIAKAKA GE LKWEDI KMKYSWNV CEVLR+ASP+QGAFREA
Sbjct: 361 LAELPQIYDGVYREQMEIAKAKAAGELLKWEDIQKMKYSWNVGCEVLRLASPLQGAFREA 388
Query: 477 LNDFVFNGFYIPKGWKVIFSPPS 500
+N FVFNGFYIPKGWKV +S S
Sbjct: 421 INHFVFNGFYIPKGWKVYWSSNS 388
BLAST of CaUC03G061480 vs. NCBI nr
Match:
XP_022960871.1 (beta-amyrin 28-oxidase [Cucurbita moschata])
HSP 1 Score: 652.9 bits (1683), Expect = 2.3e-183
Identity = 330/443 (74.49%), Postives = 355/443 (80.14%), Query Frame = 0
Query: 57 MEHFLISLLILFLFLLSLTFFILFHKHKSLFSYPNVPPGAIGFPILGESLEFLSTGWKGH 116
M FLISLLILFL L+SLT F+LF+KHKSLFSYPN PPGAIG PI+GESLEFLSTGWKG
Sbjct: 1 MADFLISLLILFLSLISLTLFVLFYKHKSLFSYPNTPPGAIGLPIIGESLEFLSTGWKGE 60
Query: 117 PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPS 176
PEKFIFDRLNKYKSD+FKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFP+
Sbjct: 61 PEKFIFDRLNKYKSDIFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPA 120
Query: 177 STQTNSKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVFPLAKSL 236
ST+ NSKE A +LKK LP+FLKPEALQRYIG+MDELAQRHFAS WENRE+VLVFPLAKS
Sbjct: 121 STEINSKESAIRLKKLLPRFLKPEALQRYIGVMDELAQRHFASSWENREQVLVFPLAKS- 180
Query: 237 IEIQFLQSLILVDRLPIMELVSCSRSISISASSQSRSISFSVLIFLKLISDEIRFTFSLA 296
FTFSLA
Sbjct: 181 ------------------------------------------------------FTFSLA 240
Query: 297 CRLFVSVDDEIQVERLSRPFEHIVAGIISLPIDLPGTPFNRAIKASNFIRKELVAIVRQR 356
CRLFVS++D I VERLS PF+ I AGIIS+PIDLPGTPFNRAIKASNF+RKEL++IVRQR
Sbjct: 241 CRLFVSLEDRIHVERLSEPFQSIAAGIISMPIDLPGTPFNRAIKASNFVRKELLSIVRQR 300
Query: 357 KWDLAEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVACTFILKF 416
K DLAEGKASPTQDILSHMLLTCDE G YMNES IAD ILGLLIGGHDTTS ACTFI+ +
Sbjct: 301 KSDLAEGKASPTQDILSHMLLTCDEIGQYMNESAIADNILGLLIGGHDTTSAACTFIVNY 360
Query: 417 LAELPHVYDGVYAEQMEIAKAKAPGEALKWEDINKMKYSWNVACEVLRIASPVQGAFREA 476
LAELP +YDGVY EQMEIAKAKA GE LKWEDI KMKYSWNVACEVLR+ASP+QGAFREA
Sbjct: 361 LAELPQIYDGVYREQMEIAKAKAAGELLKWEDIQKMKYSWNVACEVLRLASPLQGAFREA 388
Query: 477 LNDFVFNGFYIPKGWKVIFSPPS 500
+N FVFNGFYIPKGWKV +S S
Sbjct: 421 INHFVFNGFYIPKGWKVYWSSNS 388
BLAST of CaUC03G061480 vs. ExPASy Swiss-Prot
Match:
F6H9N6 (Beta-amyrin 28-monooxygenase OS=Vitis vinifera OX=29760 GN=CYP716A15 PE=1 SV=1)
HSP 1 Score: 556.2 bits (1432), Expect = 3.8e-157
Identity = 271/443 (61.17%), Postives = 333/443 (75.17%), Query Frame = 0
Query: 57 MEHFLISLLILFLFLLSLTFFILFHKHKSLFSYPNVPPGAIGFPILGESLEFLSTGWKGH 116
ME F +SLL++F+ +S+ +LF+KH+S F+ PN+PPG IG+P++GESLEFLSTGWKGH
Sbjct: 1 MEVFFLSLLLIFVLSVSIGLHLLFYKHRSHFTGPNLPPGKIGWPMVGESLEFLSTGWKGH 60
Query: 117 PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPS 176
PEKFIFDR++KY S+VFKTS++G PAA+F G A NKFLFSNENKLV +WWP SV+K+FPS
Sbjct: 61 PEKFIFDRISKYSSEVFKTSLLGEPAAVFAGAAGNKFLFSNENKLVHAWWPSSVDKVFPS 120
Query: 177 STQTNSKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVFPLAKSL 236
STQT+SKEEAKK++K LPQF KPEALQRYIGIMD +AQRHFA W+NR+EV+VFPLAK
Sbjct: 121 STQTSSKEEAKKMRKLLPQFFKPEALQRYIGIMDHIAQRHFADSWDNRDEVIVFPLAK-- 180
Query: 237 IEIQFLQSLILVDRLPIMELVSCSRSISISASSQSRSISFSVLIFLKLISDEIRFTFSLA 296
RFTF LA
Sbjct: 181 -----------------------------------------------------RFTFWLA 240
Query: 297 CRLFVSVDDEIQVERLSRPFEHIVAGIISLPIDLPGTPFNRAIKASNFIRKELVAIVRQR 356
CRLF+S++D V + +PF + +G+I++PIDLPGTPF+RAIKASNFIRKEL AI++QR
Sbjct: 241 CRLFMSIEDPAHVAKFEKPFHVLASGLITVPIDLPGTPFHRAIKASNFIRKELRAIIKQR 300
Query: 357 KWDLAEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVACTFILKF 416
K DLAEGKAS QDILSHMLL DE+G +MNE +IADKILGLLIGGHDT S A TF++K+
Sbjct: 301 KIDLAEGKASQNQDILSHMLLATDEDGCHMNEMEIADKILGLLIGGHDTASAAITFLIKY 360
Query: 417 LAELPHVYDGVYAEQMEIAKAKAPGEALKWEDINKMKYSWNVACEVLRIASPVQGAFREA 476
+AELPH+Y+ VY EQMEIA +KAPGE L W+D+ KM+YSWNVACEV+R+A P+QGAFREA
Sbjct: 361 MAELPHIYEKVYEEQMEIANSKAPGELLNWDDVQKMRYSWNVACEVMRLAPPLQGAFREA 388
Query: 477 LNDFVFNGFYIPKGWKVIFSPPS 500
+ DFVFNGF IPKGWK+ +S S
Sbjct: 421 ITDFVFNGFSIPKGWKLYWSANS 388
BLAST of CaUC03G061480 vs. ExPASy Swiss-Prot
Match:
A5BFI4 (Beta-amyrin 28-monooxygenase OS=Vitis vinifera OX=29760 GN=CYP716A17 PE=1 SV=2)
HSP 1 Score: 550.4 bits (1417), Expect = 2.1e-155
Identity = 269/443 (60.72%), Postives = 331/443 (74.72%), Query Frame = 0
Query: 57 MEHFLISLLILFLFLLSLTFFILFHKHKSLFSYPNVPPGAIGFPILGESLEFLSTGWKGH 116
ME F +SLL++ + +S+ ++L +KH+S F+ PN+PPG IG+P++GESLEFLSTGWKGH
Sbjct: 1 MEVFFLSLLLICVLSVSIRLYLLLYKHRSHFTGPNLPPGKIGWPMVGESLEFLSTGWKGH 60
Query: 117 PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPS 176
PEKFIFDR++KY S+VFKTS++G PAA+F G A NKFLFSNENKLV +WWP SV+K+FPS
Sbjct: 61 PEKFIFDRISKYSSEVFKTSLLGEPAAVFAGAAGNKFLFSNENKLVHAWWPSSVDKVFPS 120
Query: 177 STQTNSKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVFPLAKSL 236
STQT+SKEEAKK++K LPQFLKPEALQRY GIMD +AQRHFA W+NR+EV+VFPLAK
Sbjct: 121 STQTSSKEEAKKMRKLLPQFLKPEALQRYTGIMDHIAQRHFADSWDNRDEVIVFPLAK-- 180
Query: 237 IEIQFLQSLILVDRLPIMELVSCSRSISISASSQSRSISFSVLIFLKLISDEIRFTFSLA 296
RFTF LA
Sbjct: 181 -----------------------------------------------------RFTFWLA 240
Query: 297 CRLFVSVDDEIQVERLSRPFEHIVAGIISLPIDLPGTPFNRAIKASNFIRKELVAIVRQR 356
CRLF+S++D V + +PF + +G+I++PIDLPGTPF+RAIKASNFIRKEL AI++QR
Sbjct: 241 CRLFMSIEDPAHVAKFEKPFHVLASGLITIPIDLPGTPFHRAIKASNFIRKELRAIIKQR 300
Query: 357 KWDLAEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVACTFILKF 416
K DLAE KAS TQDILSHMLL DE+G +MNE IADKILGLLIGGHDT S A TF++K+
Sbjct: 301 KIDLAESKASKTQDILSHMLLATDEDGCHMNEMSIADKILGLLIGGHDTASSAITFLVKY 360
Query: 417 LAELPHVYDGVYAEQMEIAKAKAPGEALKWEDINKMKYSWNVACEVLRIASPVQGAFREA 476
+AELPH+Y+ VY EQMEIA +KAPGE L W+D+ KM+YSWNVACEV+R+A P+QGAFREA
Sbjct: 361 MAELPHIYEKVYKEQMEIANSKAPGELLNWDDVQKMRYSWNVACEVMRLAPPLQGAFREA 388
Query: 477 LNDFVFNGFYIPKGWKVIFSPPS 500
+ DFVFNGF IPKGWK+ +S S
Sbjct: 421 ITDFVFNGFSIPKGWKLYWSANS 388
BLAST of CaUC03G061480 vs. ExPASy Swiss-Prot
Match:
A0A0S2II38 (Beta-amyrin 28-monooxygenase OS=Kalopanax septemlobus OX=228393 GN=CYP716A94 PE=1 SV=1)
HSP 1 Score: 539.3 bits (1388), Expect = 4.8e-152
Identity = 270/444 (60.81%), Postives = 323/444 (72.75%), Query Frame = 0
Query: 57 MEHFLISLLILFLFLLSLTFFILFHKHKS-LFSYPNVPPGAIGFPILGESLEFLSTGWKG 116
M+ F + LL LF+ +SL+F+ LF+K KS S +PPG G+P++GES EFLSTGWKG
Sbjct: 1 MQLFYVPLLSLFVLFVSLSFYFLFYKSKSGSTSGLPLPPGKTGWPVIGESFEFLSTGWKG 60
Query: 117 HPEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFP 176
+PEKFIFDRL KY S+VFKTS+ G PAA FCG ACNKFLFSNENKLV +WWPDSVNK+FP
Sbjct: 61 YPEKFIFDRLAKYSSNVFKTSLFGQPAAAFCGAACNKFLFSNENKLVQAWWPDSVNKVFP 120
Query: 177 SSTQTNSKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVFPLAKS 236
SSTQT+SKEEA K++K LP FLKPEALQRY+G+MD++A +HF S WEN++EVLVFPLAK+
Sbjct: 121 SSTQTSSKEEAIKMRKLLPNFLKPEALQRYVGVMDQIATKHFKSGWENKKEVLVFPLAKN 180
Query: 237 LIEIQFLQSLILVDRLPIMELVSCSRSISISASSQSRSISFSVLIFLKLISDEIRFTFSL 296
+TF +
Sbjct: 181 -------------------------------------------------------YTFWI 240
Query: 297 ACRLFVSVDDEIQVERLSRPFEHIVAGIISLPIDLPGTPFNRAIKASNFIRKELVAIVRQ 356
AC++FVSV++ QV +L PF I +GIIS+PIDLPGTPFN AIK+S +R +L+ I++Q
Sbjct: 241 ACKVFVSVEEPTQVAKLLEPFNAIASGIISVPIDLPGTPFNSAIKSSKIVRDKLMGIIKQ 300
Query: 357 RKWDLAEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVACTFILK 416
RK DL EGKASPTQDILSHMLLT DENG +M E DIADKILGLLIGGHDT S ACTF++K
Sbjct: 301 RKTDLGEGKASPTQDILSHMLLTSDENGKFMTEGDIADKILGLLIGGHDTASSACTFVVK 360
Query: 417 FLAELPHVYDGVYAEQMEIAKAKAPGEALKWEDINKMKYSWNVACEVLRIASPVQGAFRE 476
FLAELP +Y+GVY EQMEIAK+K GE L WEDI KMKYSWNVACEVLR+A P+QGAFRE
Sbjct: 361 FLAELPEIYEGVYKEQMEIAKSKKAGELLNWEDIQKMKYSWNVACEVLRLAPPLQGAFRE 389
Query: 477 ALNDFVFNGFYIPKGWKVIFSPPS 500
AL DF +NGF IPKGWK+ +S S
Sbjct: 421 ALTDFSYNGFSIPKGWKLYWSANS 389
BLAST of CaUC03G061480 vs. ExPASy Swiss-Prot
Match:
A0A3Q7HBJ5 (Beta-amyrin 28-monooxygenase OS=Solanum lycopersicum OX=4081 GN=CYP716A44 PE=1 SV=1)
HSP 1 Score: 538.1 bits (1385), Expect = 1.1e-151
Identity = 268/443 (60.50%), Postives = 323/443 (72.91%), Query Frame = 0
Query: 57 MEHFLISLLILFLFLLSLTFFILFHKHKSLFSYPNVPPGAIGFPILGESLEFLSTGWKGH 116
ME + L+ +F+FL+SL +L +K K S +PPG G+P+ GESLEFLS+GWKGH
Sbjct: 1 MELLYVCLVCVFVFLVSL---LLLYKKK---SGEGLPPGKTGWPVFGESLEFLSSGWKGH 60
Query: 117 PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPS 176
PEKFIFDR+ KY S VFKT ++G AA+FCG + NKFLFSNENKLV +WWP+SVNK+FPS
Sbjct: 61 PEKFIFDRVAKYSSSVFKTHLLGEEAAVFCGASANKFLFSNENKLVQAWWPNSVNKVFPS 120
Query: 177 STQTNSKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVFPLAKSL 236
STQT+SKEEA K++K LP F KPEALQRY+GIMD + QRHFA+ WEN+E+V+VFPL K
Sbjct: 121 STQTSSKEEAIKMRKMLPNFFKPEALQRYVGIMDHITQRHFATGWENKEQVVVFPLTK-- 180
Query: 237 IEIQFLQSLILVDRLPIMELVSCSRSISISASSQSRSISFSVLIFLKLISDEIRFTFSLA 296
R+TF LA
Sbjct: 181 -----------------------------------------------------RYTFWLA 240
Query: 297 CRLFVSVDDEIQVERLSRPFEHIVAGIISLPIDLPGTPFNRAIKASNFIRKELVAIVRQR 356
CRLF+SV+D V + + PF+ + +G+IS+PIDLPGTPFNRAIKASNFIRKELV I++QR
Sbjct: 241 CRLFLSVEDPKHVAKFADPFDVLASGLISIPIDLPGTPFNRAIKASNFIRKELVRIIKQR 300
Query: 357 KWDLAEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVACTFILKF 416
K DL EGK S TQDILSHMLLTCDENG ++ + DIADKILGLLIGGHDT S AC+FI+K+
Sbjct: 301 KIDLGEGKVSSTQDILSHMLLTCDENGKFLGDLDIADKILGLLIGGHDTASSACSFIVKY 360
Query: 417 LAELPHVYDGVYAEQMEIAKAKAPGEALKWEDINKMKYSWNVACEVLRIASPVQGAFREA 476
LAELPH+Y VY EQMEIAK+K PGE L+WEDI KMKYSWNVACEVLR+A P+QGAFREA
Sbjct: 361 LAELPHIYQRVYTEQMEIAKSKGPGELLRWEDIQKMKYSWNVACEVLRLAPPLQGAFREA 382
Query: 477 LNDFVFNGFYIPKGWKVIFSPPS 500
L+DF+FNGFYIPKGWK+ +S S
Sbjct: 421 LSDFMFNGFYIPKGWKIYWSANS 382
BLAST of CaUC03G061480 vs. ExPASy Swiss-Prot
Match:
K4CEE8 (Beta-amyrin 28-monooxygenase OS=Solanum lycopersicum OX=4081 GN=CYP716A46 PE=1 SV=1)
HSP 1 Score: 538.1 bits (1385), Expect = 1.1e-151
Identity = 275/444 (61.94%), Postives = 327/444 (73.65%), Query Frame = 0
Query: 57 MEHFLISLLILFLFLLSLTFFILFH-KHKSLFSYPNVPPGAIGFPILGESLEFLSTGWKG 116
ME F SL+ LF+ LLSL+F LF+ K+KS+ +PPG G+P++GE+LEFLSTGWKG
Sbjct: 1 MELFYASLVCLFVLLLSLSFLFLFNKKNKSMILSCPLPPGNSGWPVIGETLEFLSTGWKG 60
Query: 117 HPEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFP 176
HPEKFIFDR++KYKS +FKT ++G A +FCG + NKFLFSNENKLV +WWP SV+K+FP
Sbjct: 61 HPEKFIFDRISKYKSSIFKTHLLGEKAVVFCGASSNKFLFSNENKLVQTWWPSSVDKVFP 120
Query: 177 SSTQTNSKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVFPLAKS 236
SSTQT+SKEEA K++K LP FLKPEALQRYI IMD +AQ+HF S WEN++EV+VFPLAK
Sbjct: 121 SSTQTSSKEEAIKMRKMLPNFLKPEALQRYIRIMDHIAQKHFQS-WENQQEVVVFPLAK- 180
Query: 237 LIEIQFLQSLILVDRLPIMELVSCSRSISISASSQSRSISFSVLIFLKLISDEIRFTFSL 296
R+TF L
Sbjct: 181 ------------------------------------------------------RYTFWL 240
Query: 297 ACRLFVSVDDEIQVERLSRPFEHIVAGIISLPIDLPGTPFNRAIKASNFIRKELVAIVRQ 356
ACRLFVSV+D V RL+ PF+ + +G+IS+PI+LPGTPFNRAIKASNFIRKELVAI++Q
Sbjct: 241 ACRLFVSVEDPNHVARLADPFDVLASGLISIPINLPGTPFNRAIKASNFIRKELVAIIKQ 300
Query: 357 RKWDLAEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVACTFILK 416
RK DL EGKAS +QDILSHMLLT DENG +M+E DIADKILGLLIGGHDT S AC FI+K
Sbjct: 301 RKIDLTEGKASDSQDILSHMLLTSDENGKFMHELDIADKILGLLIGGHDTASSACAFIVK 360
Query: 417 FLAELPHVYDGVYAEQMEIAKAKAPGEALKWEDINKMKYSWNVACEVLRIASPVQGAFRE 476
+LAELP +YD VY EQ+EIAK+K PGE L WEDI KMKYSWNVACEVLR+A P+QGAFRE
Sbjct: 361 YLAELPEIYDQVYKEQIEIAKSKGPGELLSWEDIKKMKYSWNVACEVLRLAPPLQGAFRE 388
Query: 477 ALNDFVFNGFYIPKGWKVIFSPPS 500
AL DF FNGF IPKGWK+ +S S
Sbjct: 421 ALVDFTFNGFSIPKGWKIYWSANS 388
BLAST of CaUC03G061480 vs. ExPASy TrEMBL
Match:
A0A1S3B8H4 (beta-amyrin 28-oxidase OS=Cucumis melo OX=3656 GN=LOC103487187 PE=3 SV=1)
HSP 1 Score: 676.0 bits (1743), Expect = 1.2e-190
Identity = 341/443 (76.98%), Postives = 359/443 (81.04%), Query Frame = 0
Query: 57 MEHFLISLLILFLFLLSLTFFILFHKHKSLFSYPNVPPGAIGFPILGESLEFLSTGWKGH 116
MEHFLISLLIL F LSLT FILFH HKSLFSYPN PPGAIG PILGESLEFLS+GW+GH
Sbjct: 1 MEHFLISLLILLFFFLSLTLFILFHNHKSLFSYPNTPPGAIGLPILGESLEFLSSGWRGH 60
Query: 117 PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPS 176
PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGP CNKFLFSNENKLVT WWPDSVNKIFPS
Sbjct: 61 PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNKIFPS 120
Query: 177 STQTNSKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVFPLAKSL 236
+TQT+SKEEAKKLKK LPQFLKPEALQRYIGIMDELA+RHF SFW+NREEVLVFPLAKS
Sbjct: 121 TTQTSSKEEAKKLKKLLPQFLKPEALQRYIGIMDELAERHFNSFWKNREEVLVFPLAKS- 180
Query: 237 IEIQFLQSLILVDRLPIMELVSCSRSISISASSQSRSISFSVLIFLKLISDEIRFTFSLA 296
FTFSLA
Sbjct: 181 ------------------------------------------------------FTFSLA 240
Query: 297 CRLFVSVDDEIQVERLSRPFEHIVAGIISLPIDLPGTPFNRAIKASNFIRKELVAIVRQR 356
CRLF+SV+DEI VERLS PFEHI AGIIS+PIDLPGTPFNRAIKAS FIRKE+VAIVRQR
Sbjct: 241 CRLFMSVEDEIHVERLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEVVAIVRQR 300
Query: 357 KWDLAEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVACTFILKF 416
K DLAEGKA TQDILSHMLLTCDENG YMNESDI DKILGLLIGGHDT SVACTFI+KF
Sbjct: 301 KQDLAEGKALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVACTFIVKF 360
Query: 417 LAELPHVYDGVYAEQMEIAKAKAPGEALKWEDINKMKYSWNVACEVLRIASPVQGAFREA 476
LAELPH+YD VY EQMEIA+AKA GE LKWEDI KMKYSWNVACEVLR+ASP+QGAFREA
Sbjct: 361 LAELPHIYDAVYTEQMEIARAKAEGETLKWEDIKKMKYSWNVACEVLRLASPLQGAFREA 388
Query: 477 LNDFVFNGFYIPKGWKVIFSPPS 500
LNDFVFNGF+IPKGWK+ +S S
Sbjct: 421 LNDFVFNGFFIPKGWKLYWSANS 388
BLAST of CaUC03G061480 vs. ExPASy TrEMBL
Match:
A0A0A0LXN3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G649550 PE=3 SV=1)
HSP 1 Score: 668.7 bits (1724), Expect = 1.9e-188
Identity = 338/443 (76.30%), Postives = 358/443 (80.81%), Query Frame = 0
Query: 57 MEHFLISLLILFLFLLSLTFFILFHKHKSLFSYPNVPPGAIGFPILGESLEFLSTGWKGH 116
ME FLISLLIL F LSLT FILFH HKSLFSYPN PPGAIG PILGES+EFLS+GWKGH
Sbjct: 1 MELFLISLLILLFFFLSLTLFILFHNHKSLFSYPNTPPGAIGLPILGESVEFLSSGWKGH 60
Query: 117 PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPS 176
PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGP CNKFLFSNENKLVT WWPDSVNKIFPS
Sbjct: 61 PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNKIFPS 120
Query: 177 STQTNSKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVFPLAKSL 236
+TQT++KEEAKKLKK LPQFLKPEALQRYIGIMDELA+RHF SFW+NREEVLVFPLAKS
Sbjct: 121 TTQTSTKEEAKKLKKLLPQFLKPEALQRYIGIMDELAERHFNSFWKNREEVLVFPLAKS- 180
Query: 237 IEIQFLQSLILVDRLPIMELVSCSRSISISASSQSRSISFSVLIFLKLISDEIRFTFSLA 296
FTFS+A
Sbjct: 181 ------------------------------------------------------FTFSIA 240
Query: 297 CRLFVSVDDEIQVERLSRPFEHIVAGIISLPIDLPGTPFNRAIKASNFIRKELVAIVRQR 356
CRLF+SV+DEI VERLS PFEHI AGIIS+PIDLPGTPFNRAIKAS FIRKE+VAIVRQR
Sbjct: 241 CRLFMSVEDEIHVERLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEVVAIVRQR 300
Query: 357 KWDLAEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVACTFILKF 416
K DLAEGKA TQDILSHMLLTCDENG YMNESDI DKILGLLIGGHDT SVACTFI+KF
Sbjct: 301 KQDLAEGKALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVACTFIVKF 360
Query: 417 LAELPHVYDGVYAEQMEIAKAKAPGEALKWEDINKMKYSWNVACEVLRIASPVQGAFREA 476
LAELPH+YD VY EQMEIA+AKA GE LKWEDI KMKYSWNVACEVLRIASP+QGAFREA
Sbjct: 361 LAELPHIYDAVYTEQMEIARAKAEGETLKWEDIKKMKYSWNVACEVLRIASPLQGAFREA 388
Query: 477 LNDFVFNGFYIPKGWKVIFSPPS 500
L+DFVFNGF+IPKGWK+ +S S
Sbjct: 421 LSDFVFNGFFIPKGWKLYWSANS 388
BLAST of CaUC03G061480 vs. ExPASy TrEMBL
Match:
A0A6J1H8T4 (beta-amyrin 28-oxidase OS=Cucurbita moschata OX=3662 GN=LOC111461545 PE=3 SV=1)
HSP 1 Score: 652.9 bits (1683), Expect = 1.1e-183
Identity = 330/443 (74.49%), Postives = 355/443 (80.14%), Query Frame = 0
Query: 57 MEHFLISLLILFLFLLSLTFFILFHKHKSLFSYPNVPPGAIGFPILGESLEFLSTGWKGH 116
M FLISLLILFL L+SLT F+LF+KHKSLFSYPN PPGAIG PI+GESLEFLSTGWKG
Sbjct: 1 MADFLISLLILFLSLISLTLFVLFYKHKSLFSYPNTPPGAIGLPIIGESLEFLSTGWKGE 60
Query: 117 PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPS 176
PEKFIFDRLNKYKSD+FKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFP+
Sbjct: 61 PEKFIFDRLNKYKSDIFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPA 120
Query: 177 STQTNSKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVFPLAKSL 236
ST+ NSKE A +LKK LP+FLKPEALQRYIG+MDELAQRHFAS WENRE+VLVFPLAKS
Sbjct: 121 STEINSKESAIRLKKLLPRFLKPEALQRYIGVMDELAQRHFASSWENREQVLVFPLAKS- 180
Query: 237 IEIQFLQSLILVDRLPIMELVSCSRSISISASSQSRSISFSVLIFLKLISDEIRFTFSLA 296
FTFSLA
Sbjct: 181 ------------------------------------------------------FTFSLA 240
Query: 297 CRLFVSVDDEIQVERLSRPFEHIVAGIISLPIDLPGTPFNRAIKASNFIRKELVAIVRQR 356
CRLFVS++D I VERLS PF+ I AGIIS+PIDLPGTPFNRAIKASNF+RKEL++IVRQR
Sbjct: 241 CRLFVSLEDRIHVERLSEPFQSIAAGIISMPIDLPGTPFNRAIKASNFVRKELLSIVRQR 300
Query: 357 KWDLAEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVACTFILKF 416
K DLAEGKASPTQDILSHMLLTCDE G YMNES IAD ILGLLIGGHDTTS ACTFI+ +
Sbjct: 301 KSDLAEGKASPTQDILSHMLLTCDEIGQYMNESAIADNILGLLIGGHDTTSAACTFIVNY 360
Query: 417 LAELPHVYDGVYAEQMEIAKAKAPGEALKWEDINKMKYSWNVACEVLRIASPVQGAFREA 476
LAELP +YDGVY EQMEIAKAKA GE LKWEDI KMKYSWNVACEVLR+ASP+QGAFREA
Sbjct: 361 LAELPQIYDGVYREQMEIAKAKAAGELLKWEDIQKMKYSWNVACEVLRLASPLQGAFREA 388
Query: 477 LNDFVFNGFYIPKGWKVIFSPPS 500
+N FVFNGFYIPKGWKV +S S
Sbjct: 421 INHFVFNGFYIPKGWKVYWSSNS 388
BLAST of CaUC03G061480 vs. ExPASy TrEMBL
Match:
A0A6J1DZX7 (beta-amyrin 28-oxidase OS=Momordica charantia OX=3673 GN=LOC111024635 PE=3 SV=1)
HSP 1 Score: 649.4 bits (1674), Expect = 1.2e-182
Identity = 322/443 (72.69%), Postives = 351/443 (79.23%), Query Frame = 0
Query: 57 MEHFLISLLILFLFLLSLTFFILFHKHKSLFSYPNVPPGAIGFPILGESLEFLSTGWKGH 116
MEHFL+SLLI F+ ++ + FILF+KHKS FSYPN+PPG IGFPI+GESLEF STGWKG
Sbjct: 1 MEHFLVSLLIFFVSVVCVGLFILFYKHKSPFSYPNIPPGGIGFPIIGESLEFFSTGWKGE 60
Query: 117 PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPS 176
PEKFIFDR+NKYKS +FKTSIVG PAA+FCGP CNKFLFSNENKLVT+WWPDSVNKIFPS
Sbjct: 61 PEKFIFDRINKYKSHIFKTSIVGAPAAVFCGPTCNKFLFSNENKLVTAWWPDSVNKIFPS 120
Query: 177 STQTNSKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVFPLAKSL 236
STQT+S EEAKKLKK LPQFLKPEAL RYIGIMD LA RHFAS WEN+++VLVFPLAKS
Sbjct: 121 STQTSSMEEAKKLKKLLPQFLKPEALHRYIGIMDALAHRHFASSWENKDQVLVFPLAKS- 180
Query: 237 IEIQFLQSLILVDRLPIMELVSCSRSISISASSQSRSISFSVLIFLKLISDEIRFTFSLA 296
FTFSLA
Sbjct: 181 ------------------------------------------------------FTFSLA 240
Query: 297 CRLFVSVDDEIQVERLSRPFEHIVAGIISLPIDLPGTPFNRAIKASNFIRKELVAIVRQR 356
CRLFVSV+DE VERLS PF+HI AGIIS+PID PGTPFNR IKASNFIRKEL++IV+QR
Sbjct: 241 CRLFVSVEDEAHVERLSGPFQHIAAGIISMPIDFPGTPFNRGIKASNFIRKELLSIVKQR 300
Query: 357 KWDLAEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVACTFILKF 416
K DLAEGKASPTQDILSHMLLTCDENG YMNE DIADKILGLLIGGHDT S ACTFI+KF
Sbjct: 301 KVDLAEGKASPTQDILSHMLLTCDENGAYMNELDIADKILGLLIGGHDTASAACTFIVKF 360
Query: 417 LAELPHVYDGVYAEQMEIAKAKAPGEALKWEDINKMKYSWNVACEVLRIASPVQGAFREA 476
LAELPH+YDGVY EQM IAKAKAPGE L WEDI KMKYSWNVACEVLR+ASP+QGAFREA
Sbjct: 361 LAELPHIYDGVYKEQMAIAKAKAPGELLNWEDIQKMKYSWNVACEVLRLASPLQGAFREA 388
Query: 477 LNDFVFNGFYIPKGWKVIFSPPS 500
+NDFVFNGFYIPKGWK+ +S S
Sbjct: 421 INDFVFNGFYIPKGWKLYWSSNS 388
BLAST of CaUC03G061480 vs. ExPASy TrEMBL
Match:
A0A6J1JJQ7 (beta-amyrin 28-oxidase OS=Cucurbita maxima OX=3661 GN=LOC111485071 PE=3 SV=1)
HSP 1 Score: 641.0 bits (1652), Expect = 4.3e-180
Identity = 326/445 (73.26%), Postives = 353/445 (79.33%), Query Frame = 0
Query: 57 MEHFLISLLILFLFLLSLTFFILFHKHKSLFSYPNVPPGAIGFPILGESLEFLSTGWKGH 116
ME FLISLLILFL +SLT F+LF+KHKSLFSYPN PPGAIG PI+GESLEFLSTGWKG
Sbjct: 1 MEDFLISLLILFLSFISLTLFVLFYKHKSLFSYPNTPPGAIGLPIIGESLEFLSTGWKGE 60
Query: 117 PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPS 176
PEKFIFDRLNKYKSD+FKTSIVGVPAAIFCGP CNKFLFSNENKLVTSWWPDSVNKIFP+
Sbjct: 61 PEKFIFDRLNKYKSDIFKTSIVGVPAAIFCGPVCNKFLFSNENKLVTSWWPDSVNKIFPA 120
Query: 177 STQTNSKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVFPLAKSL 236
ST+ NSKE A +LKK LP+FLKPEALQRYIG+MDELAQRHFAS WENRE VLVFPLAKS
Sbjct: 121 STEINSKESAIRLKKLLPRFLKPEALQRYIGVMDELAQRHFASSWENREHVLVFPLAKS- 180
Query: 237 IEIQFLQSLILVDRLPIMELVSCSRSISISASSQSRSISFSVLIFLKLISDEIRFTFSLA 296
FTFSLA
Sbjct: 181 ------------------------------------------------------FTFSLA 240
Query: 297 CRLFVSVDDEIQVERLSRPFEHIVAGIISLPIDLPGTPFNRAIKASNFIRKELVAIVRQR 356
CRLF+S++D I VERLS PF++I AGIIS+PIDLPGTPFNRAIKASNF+RKEL++IVRQR
Sbjct: 241 CRLFLSLEDRIHVERLSEPFQNIAAGIISMPIDLPGTPFNRAIKASNFVRKELLSIVRQR 300
Query: 357 KWDLAEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVACTFILKF 416
K DLAEGKASPTQDILSHMLLTCDE G YMNES IAD ILGLLIGGHDT S ACTFI+K+
Sbjct: 301 KSDLAEGKASPTQDILSHMLLTCDEIGQYMNESAIADNILGLLIGGHDTASAACTFIVKY 360
Query: 417 LAELPHVYDGVYAEQMEI--AKAKAPGEALKWEDINKMKYSWNVACEVLRIASPVQGAFR 476
LAELP +YD VY EQMEI AKAKA GE LKWEDI KMKYSWNVACEVLR+ASP+QGAFR
Sbjct: 361 LAELPQIYDRVYREQMEIAKAKAKAKGELLKWEDIQKMKYSWNVACEVLRLASPLQGAFR 390
Query: 477 EALNDFVFNGFYIPKGWKVIFSPPS 500
EA+NDFVFNGF IPKGWK+ +S S
Sbjct: 421 EAINDFVFNGFNIPKGWKLYWSSNS 390
BLAST of CaUC03G061480 vs. TAIR 10
Match:
AT5G36110.1 (cytochrome P450, family 716, subfamily A, polypeptide 1 )
HSP 1 Score: 448.7 bits (1153), Expect = 6.0e-126
Identity = 227/440 (51.59%), Postives = 286/440 (65.00%), Query Frame = 0
Query: 60 FLISLLILFLFLLSLTFFILFHKHKSLFSYPNVPPGAIGFPILGESLEFLSTGWKGHPEK 119
++ ++ILFL + L+ +L KH S FSYPN+PPG G P++GES FLS G +GHPEK
Sbjct: 2 YMAIMIILFLSSILLSLLLLLRKHLSHFSYPNLPPGNTGLPLIGESFSFLSAGRQGHPEK 61
Query: 120 FIFDRLNKYKSD---VFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPS 179
FI DR+ ++ S VFKT + G P A+ G + NKFLF+NENKLV SWWPDSVNKIFPS
Sbjct: 62 FITDRVRRFSSSSSCVFKTHLFGSPTAVVTGASGNKFLFTNENKLVVSWWPDSVNKIFPS 121
Query: 180 STQTNSKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVFPLAKSL 239
S QT+SKEEA+KL+ L QF+KPEAL+RY+G+MDE+AQRHF + W N+++V+VFPL K
Sbjct: 122 SMQTSSKEEARKLRMLLSQFMKPEALRRYVGVMDEIAQRHFETEWANQDQVIVFPLTK-- 181
Query: 240 IEIQFLQSLILVDRLPIMELVSCSRSISISASSQSRSISFSVLIFLKLISDEIRFTFSLA 299
+FTFS+A
Sbjct: 182 -----------------------------------------------------KFTFSIA 241
Query: 300 CRLFVSVDDEIQVERLSRPFEHIVAGIISLPIDLPGTPFNRAIKASNFIRKELVAIVRQR 359
CR F+S++D +V +L F + GI S+PIDLPGT FNRAIKAS +RKE+ AIVRQR
Sbjct: 242 CRSFLSMEDPARVRQLEEQFNTVAVGIFSIPIDLPGTRFNRAIKASRLLRKEVSAIVRQR 301
Query: 360 KWDLAEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVACTFILKF 419
K +L GKA DILSHML+ E + D+ADKI+GLLIGGHDT S+ CTF++ +
Sbjct: 302 KEELKAGKALEEHDILSHMLMNIGET----KDEDLADKIIGLLIGGHDTASIVCTFVVNY 361
Query: 420 LAELPHVYDGVYAEQMEIAKAKAPGEALKWEDINKMKYSWNVACEVLRIASPVQGAFREA 479
LAE PHVY V EQ EI K K E L+WEDI KM+YSWNVACEV+RI P+ G FREA
Sbjct: 362 LAEFPHVYQRVLQEQKEILKEKKEKEGLRWEDIEKMRYSWNVACEVMRIVPPLSGTFREA 382
Query: 480 LNDFVFNGFYIPKGWKVIFS 497
++ F F GFYIPKGWK+ +S
Sbjct: 422 IDHFSFKGFYIPKGWKLYWS 382
BLAST of CaUC03G061480 vs. TAIR 10
Match:
AT5G36140.1 (cytochrome P450, family 716, subfamily A, polypeptide 2 )
HSP 1 Score: 353.6 bits (906), Expect = 2.6e-97
Identity = 184/373 (49.33%), Postives = 239/373 (64.08%), Query Frame = 0
Query: 64 LLILFLFLLSLTFFILFH--KHKSLFSYPNVPPGAIGFPILGESLEFLSTGWKGHPEKFI 123
L I+FLF+ S+ F +LF KH S F YPN+PPG IGFP++GE+L FLS G +GHPEKF+
Sbjct: 3 LTIIFLFISSIIFPLLFFLGKHLSNFRYPNLPPGKIGFPLIGETLSFLSAGRQGHPEKFV 62
Query: 124 FDRLNKYKSDVFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPSSTQTN 183
DR+ + S +FKT + G P A+ G + NKFLF+NENKLV SWWPDSVNKIFPSSTQT+
Sbjct: 63 TDRVRHFSSGIFKTHLFGSPFAVVTGASGNKFLFTNENKLVISWWPDSVNKIFPSSTQTS 122
Query: 184 SKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVFPLAKSLIEIQF 243
SKEEA K + L +KPEAL+RY+G+MDE+AQ+HF + W N+++++VFPL K
Sbjct: 123 SKEEAIKTRMLLMPSMKPEALRRYVGVMDEIAQKHFETEWANQDQLIVFPLTK------- 182
Query: 244 LQSLILVDRLPIMELVSCSRSISISASSQSRSISFSVLIFLKLISDEIRFTFSLACRLFV 303
+FTFS+ACRLF+
Sbjct: 183 ------------------------------------------------KFTFSIACRLFL 242
Query: 304 SVDDEIQVERLSRPFEHIVAGIISLPIDLPGTPFNRAIKASNFIRKELVAIVRQRKWDLA 363
S+DD +V +L PF ++ G+ S+PIDLPGT FNRAIKAS + KE+ I+RQRK +L
Sbjct: 243 SMDDLERVRKLEEPFTTVMTGVFSIPIDLPGTRFNRAIKASRLLSKEVSTIIRQRKEELK 302
Query: 364 EGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVACTFILKFLAELP 423
GK S QDILSHML+ E + D+ADKI+ LLIGGHDTTS+ CTF++ +LAE P
Sbjct: 303 AGKVSVEQDILSHMLMNIGET----KDEDLADKIIALLIGGHDTTSIVCTFVVNYLAEFP 315
Query: 424 HVYDGVYAEQMEI 435
H+Y V E M+I
Sbjct: 363 HIYQRV-LEGMQI 315
BLAST of CaUC03G061480 vs. TAIR 10
Match:
AT2G42850.1 (cytochrome P450, family 718 )
HSP 1 Score: 157.9 bits (398), Expect = 2.1e-38
Identity = 118/447 (26.40%), Postives = 200/447 (44.74%), Query Frame = 0
Query: 59 HFLISLLILFL-FLLSLTFFILFHKH-----KSLFSYPNVPPGAIGFPILGESLEFLSTG 118
+F++S + L + +S T F KH K + + PG +G P +GE+++F
Sbjct: 6 NFVLSWVFLCIAATISSTLFFFRKKHHRFITKKIQKKKKLLPGEMGLPWIGETMDFYKAQ 65
Query: 119 WKGHP-EKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWP-DSV 178
E F+ R+ K+ ++FKT I+G P + G N+ + SNE LV S WP SV
Sbjct: 66 KSNRVFEDFVNPRIIKH-GNIFKTRIMGSPTIVVNGAEANRLILSNEFSLVVSSWPSSSV 125
Query: 179 NKIFPSSTQTNSKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVF 238
+ + E+ + L+ + L L+ I + + + H + W +EE+ ++
Sbjct: 126 QLMGMNCIMAKQGEKHRVLRGIVANSLSYIGLESLIPKLCDTVKFHHETEWRGKEEISLY 185
Query: 239 PLAKSLIEIQFLQSLILVDRLPIMELVSCSRSISISASSQSRSISFSVLIFLKLISDEIR 298
AK L
Sbjct: 186 RSAKVL------------------------------------------------------ 245
Query: 299 FTFSLACRLFVSVDDEIQVERLSRPFEHIVAGIISLPIDLPGTPFNRAIKASNFIRKELV 358
TF++ + EI + + FE ++ G+ +LP++ P + F RA KA I LV
Sbjct: 246 -TFTVVFECLYGIKVEIGMLEV---FERVLEGVFALPVEFPCSKFARAKKARLEIETFLV 305
Query: 359 AIVRQRKWDL-AEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVA 418
VR+++ ++ EG P + S ++ + + E ++ D ++ L+ HDTTS A
Sbjct: 306 GKVREKRREMEKEGAEKPNTTLFSRLVEELIKG--VITEEEVVDNMVLLVFAAHDTTSYA 365
Query: 419 CTFILKFLAELPHVYDGVYAEQMEIAKAKAPGEALKWEDINKMKYSWNVACEVLRIASPV 478
+ K LA+ P D + E +I K GE L ED+ KMKYSW V E +R++ P+
Sbjct: 366 MSMTFKMLAQHPTCRDTLLQEHAQIKANKGEGEYLTVEDVKKMKYSWQVVRETMRLSPPI 391
Query: 479 QGAFREALNDFVFNGFYIPKGWKVIFS 497
G+FR+A+ D + G+ IPKGWK++++
Sbjct: 426 FGSFRKAVADIDYGGYTIPKGWKILWT 391
BLAST of CaUC03G061480 vs. TAIR 10
Match:
AT5G45340.1 (cytochrome P450, family 707, subfamily A, polypeptide 3 )
HSP 1 Score: 151.4 bits (381), Expect = 2.0e-36
Identity = 122/434 (28.11%), Postives = 198/434 (45.62%), Query Frame = 0
Query: 62 ISLLILFLFLLSLTFFILFHKHKSLFSYPNVPPGAIGFPILGESLEFLSTGWKGHPEKFI 121
+S LFL LL FI + S P +PPG +G+P +GE+ + S P F
Sbjct: 10 LSAAALFLCLLR---FIAGVRRSSSTKLP-LPPGTMGYPYVGETFQLYSQ----DPNVFF 69
Query: 122 FDRLNKYKSDVFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIF-PSSTQT 181
+ +Y S VFKT ++G P + P KF+ ++ L +P S ++ +
Sbjct: 70 AAKQRRYGS-VFKTHVLGCPCVMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFF 129
Query: 182 NSKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVFPLAKSLIEIQ 241
+ + KL+K + + P+A++ + ++ +AQ S W+ +
Sbjct: 130 HQGDYHSKLRKLVLRAFMPDAIRNMVPHIESIAQESLNS-WDGTQ--------------- 189
Query: 242 FLQSLILVDRLPIMELVSCSRSISISASSQSRSISFSVLIFLKLISDEIRFTFSLACRLF 301
++ + ++ +F+V + L DE+ +
Sbjct: 190 ------------------------LNTYQEMKTYTFNVALISILGKDEVYYR-------- 249
Query: 302 VSVDDEIQVERLSRPFEHIVAGIISLPIDLPGTPFNRAIKASNFIRKELVAIVRQRKWDL 361
E L R + + G S+PI+LPGT F++A+KA RKEL I+
Sbjct: 250 ---------EDLKRCYYILEKGYNSMPINLPGTLFHKAMKA----RKELAQILANILSKR 309
Query: 362 AEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVACTFILKFLAEL 421
+ +S T D+L + E+ + + IAD I+G++ DTT+ T+ILK+LA+
Sbjct: 310 RQNPSSHT-DLLGSFM----EDKAGLTDEQIADNIIGVIFAARDTTASVLTWILKYLADN 368
Query: 422 PHVYDGVYAEQMEIAKAKAPGEALKWEDINKMKYSWNVACEVLRIASPVQGAFREALNDF 481
P V + V EQM I K K GE+L WED KM ++ V E LR A+ + FREA+ D
Sbjct: 370 PTVLEAVTEEQMAIRKDKKEGESLTWEDTKKMPLTYRVIQETLRAATILSFTFREAVEDV 368
Query: 482 VFNGFYIPKGWKVI 495
+ G+ IPKGWKV+
Sbjct: 430 EYEGYLIPKGWKVL 368
BLAST of CaUC03G061480 vs. TAIR 10
Match:
AT5G45340.2 (cytochrome P450, family 707, subfamily A, polypeptide 3 )
HSP 1 Score: 151.4 bits (381), Expect = 2.0e-36
Identity = 122/434 (28.11%), Postives = 198/434 (45.62%), Query Frame = 0
Query: 62 ISLLILFLFLLSLTFFILFHKHKSLFSYPNVPPGAIGFPILGESLEFLSTGWKGHPEKFI 121
+S LFL LL FI + S P +PPG +G+P +GE+ + S P F
Sbjct: 10 LSAAALFLCLLR---FIAGVRRSSSTKLP-LPPGTMGYPYVGETFQLYSQ----DPNVFF 69
Query: 122 FDRLNKYKSDVFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIF-PSSTQT 181
+ +Y S VFKT ++G P + P KF+ ++ L +P S ++ +
Sbjct: 70 AAKQRRYGS-VFKTHVLGCPCVMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFF 129
Query: 182 NSKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVFPLAKSLIEIQ 241
+ + KL+K + + P+A++ + ++ +AQ S W+ +
Sbjct: 130 HQGDYHSKLRKLVLRAFMPDAIRNMVPHIESIAQESLNS-WDGTQ--------------- 189
Query: 242 FLQSLILVDRLPIMELVSCSRSISISASSQSRSISFSVLIFLKLISDEIRFTFSLACRLF 301
++ + ++ +F+V + L DE+ +
Sbjct: 190 ------------------------LNTYQEMKTYTFNVALISILGKDEVYYR-------- 249
Query: 302 VSVDDEIQVERLSRPFEHIVAGIISLPIDLPGTPFNRAIKASNFIRKELVAIVRQRKWDL 361
E L R + + G S+PI+LPGT F++A+KA RKEL I+
Sbjct: 250 ---------EDLKRCYYILEKGYNSMPINLPGTLFHKAMKA----RKELAQILANILSKR 309
Query: 362 AEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVACTFILKFLAEL 421
+ +S T D+L + E+ + + IAD I+G++ DTT+ T+ILK+LA+
Sbjct: 310 RQNPSSHT-DLLGSFM----EDKAGLTDEQIADNIIGVIFAARDTTASVLTWILKYLADN 368
Query: 422 PHVYDGVYAEQMEIAKAKAPGEALKWEDINKMKYSWNVACEVLRIASPVQGAFREALNDF 481
P V + V EQM I K K GE+L WED KM ++ V E LR A+ + FREA+ D
Sbjct: 370 PTVLEAVTEEQMAIRKDKKEGESLTWEDTKKMPLTYRVIQETLRAATILSFTFREAVEDV 368
Query: 482 VFNGFYIPKGWKVI 495
+ G+ IPKGWKV+
Sbjct: 430 EYEGYLIPKGWKVL 368
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
XP_038877759.1 | 2.8e-194 | 78.56 | LOW QUALITY PROTEIN: beta-amyrin 28-monooxygenase-like [Benincasa hispida] | [more] |
XP_008443638.1 | 2.5e-190 | 76.98 | PREDICTED: beta-amyrin 28-oxidase [Cucumis melo] | [more] |
XP_004139087.1 | 4.0e-188 | 76.30 | beta-amyrin 28-monooxygenase [Cucumis sativus] >KGN66615.1 hypothetical protein ... | [more] |
KAG6590124.1 | 1.7e-183 | 74.49 | Beta-amyrin 28-monooxygenase, partial [Cucurbita argyrosperma subsp. sororia] >K... | [more] |
XP_022960871.1 | 2.3e-183 | 74.49 | beta-amyrin 28-oxidase [Cucurbita moschata] | [more] |
Match Name | E-value | Identity | Description | |
F6H9N6 | 3.8e-157 | 61.17 | Beta-amyrin 28-monooxygenase OS=Vitis vinifera OX=29760 GN=CYP716A15 PE=1 SV=1 | [more] |
A5BFI4 | 2.1e-155 | 60.72 | Beta-amyrin 28-monooxygenase OS=Vitis vinifera OX=29760 GN=CYP716A17 PE=1 SV=2 | [more] |
A0A0S2II38 | 4.8e-152 | 60.81 | Beta-amyrin 28-monooxygenase OS=Kalopanax septemlobus OX=228393 GN=CYP716A94 PE=... | [more] |
A0A3Q7HBJ5 | 1.1e-151 | 60.50 | Beta-amyrin 28-monooxygenase OS=Solanum lycopersicum OX=4081 GN=CYP716A44 PE=1 S... | [more] |
K4CEE8 | 1.1e-151 | 61.94 | Beta-amyrin 28-monooxygenase OS=Solanum lycopersicum OX=4081 GN=CYP716A46 PE=1 S... | [more] |
Match Name | E-value | Identity | Description | |
A0A1S3B8H4 | 1.2e-190 | 76.98 | beta-amyrin 28-oxidase OS=Cucumis melo OX=3656 GN=LOC103487187 PE=3 SV=1 | [more] |
A0A0A0LXN3 | 1.9e-188 | 76.30 | Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G649550 PE=3 SV=1 | [more] |
A0A6J1H8T4 | 1.1e-183 | 74.49 | beta-amyrin 28-oxidase OS=Cucurbita moschata OX=3662 GN=LOC111461545 PE=3 SV=1 | [more] |
A0A6J1DZX7 | 1.2e-182 | 72.69 | beta-amyrin 28-oxidase OS=Momordica charantia OX=3673 GN=LOC111024635 PE=3 SV=1 | [more] |
A0A6J1JJQ7 | 4.3e-180 | 73.26 | beta-amyrin 28-oxidase OS=Cucurbita maxima OX=3661 GN=LOC111485071 PE=3 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
AT5G36110.1 | 6.0e-126 | 51.59 | cytochrome P450, family 716, subfamily A, polypeptide 1 | [more] |
AT5G36140.1 | 2.6e-97 | 49.33 | cytochrome P450, family 716, subfamily A, polypeptide 2 | [more] |
AT2G42850.1 | 2.1e-38 | 26.40 | cytochrome P450, family 718 | [more] |
AT5G45340.1 | 2.0e-36 | 28.11 | cytochrome P450, family 707, subfamily A, polypeptide 3 | [more] |
AT5G45340.2 | 2.0e-36 | 28.11 | cytochrome P450, family 707, subfamily A, polypeptide 3 | [more] |