CaUC03G061480 (gene) Watermelon (USVL246-FR2) v1

Overview
NameCaUC03G061480
Typegene
OrganismCitrullus amarus (Watermelon (USVL246-FR2) v1)
Descriptionbeta-amyrin 28-oxidase
LocationCiama_Chr03: 24638329 .. 24644542 (+)
RNA-Seq ExpressionCaUC03G061480
SyntenyCaUC03G061480
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAAAAGTACCCATGCGCCACACACCTTGCACACGTTTTGCCATTAATCTTATTATTTTCGATTTTTGCTATATATCCAAACAAAACCTTATTTGTTGCTATTTTATCCAATTTCCCTATTTTTTATTGGACAACTCAAAATATAGCCACCTAAGGGCTTGTTTGGTTAACAATTCGGGTTCTATTTTCAAATATGTTTAACAAACAACTCGAATTTTTTTTTTTTTAAAACTATTTTCAGATTTTGTGATAAATAGTGTTAATTCTAAAAATATCACTTTTATGTTTTCATTGTTTTCATTGTATCAAAATTTTGAATATTAAATTTCAGAATGCACAATGATATTAAATAATGATAAAAATATTTAGAAATACAATTATGTTATAAACTCAATTCATTTTTTAAAAATCTAAAATATGTAATGTATTATAAATTATAGGGTTGTTTTCAAATACAACAAAATGAGCCAAAATTTTTACAAATATAGAAAATTTTCACTTTTTATCAGCGATAGACCACGATAGACTGCAATAGACTTCTATCGCAAGTCTATCGTAGTCTATTGTGAAATTTTTTTATATTTGTAAATAGTTTGATATTTTTTCTATTTATAATAATTTTCCTGAATTATATAATATTAGAAAATAATAAATATACACATAAAATATGTTTATAAATAAGATAATAATTATTTATTGTCAATTAGTATTTATTGAGTAACTACTAGCTAGTTTTATGATTTATAAATATAATATCGAACACATTTTAAACCATGGTTTAAATTAAAAGAGAAATTATTTTAAATAAAAAAACGGTTGAAAATATTTACAGATAATAGCAAAATATCACAGTCCATCTATCGCACATAAATATAATTTTTAAAAATATTTTAGTTCATTTTTCTATATTTAAAAATATAAATTAAAATTCCATTAGGTAATTGGAAAATTATAATTCTATATGCAATAGATCCAATAAATCGCCTTAGACTACTATCTCTGTCACATCGATACTAGTCTATACCAAATCTATCATCTATATTTATAAATATTTTAGTTTCTTAAGAAGAGCTCACGTATTTGTGCCTTTATATTTCCCACAAAAATGACCAATCTCCCTCCAACCTTCTTCCTCTCTGCAGTTCAAAACCTCTTTCTCAACGTAACAATGGAGCATTTCTTAATCTCTCTCTTAATCCTCTTCCTCTTTCTCCTCTCTCTCACTTTCTTCATCCTCTTCCACAAGCACAAATCCTTGTTCTCCTATCCCAACGTTCCTCCCGGCGCCATAGGCTTTCCCATTCTTGGTGAGAGCCTCGAGTTCTTGTCCACTGGCTGGAAAGGCCACCCTGAGAAGTTCATCTTCGACCGTTTGAATAAGTACAAGTCTGATGTCTTCAAAACCTCCATCGTCGGCGTTCCGGCCGCCATTTTCTGCGGCCCTGCCTGCAACAAGTTCCTCTTCTCTAACGAGAATAAACTCGTTACTTCTTGGTGGCCGGATTCCGTTAACAAGATCTTTCCCTCTTCCACTCAGACTAACTCCAAAGAGGAAGCTAAGAAACTCAAGAAATTCCTCCCGCAATTCCTTAAGCCGGAAGCGCTTCAGCGTTATATTGGAATTATGGATGAACTCGCTCAACGCCATTTCGCTTCCTTCTGGGAGAACAGGGAAGAGGTCCTTGTGTTTCCTCTCGCTAAAAGGTAAAAAATCAAACAGTTATAGTTTAATCGAAATTCAATTTCTCCAATCTTTGATTTTGGTTGATCGACTTCCGATAATGGAATTAGTTTCATGTTCTCGTTCGATTTCGATTTCTGGTGTGAATTCTGATTTCGACTTCCGATAATGGAATTAGCTTTTCTCTTTCGATTGAGATTTCGATTTCGTCTTTTGTTGCGAATTCTGATTGATCGACTTCCGAGAATGTGATTAGCTTCTTCACGTTCCCGTTCGATTTCTGCAGTGAACTCTGATTGATCGATTTCCGATGGAACTAGCTTCTTCACAATCTCGTTCGATTTCGTTTTCCGTTTTAATTTTTCTTAAACTGATTTCCGATGAAATTAGGTTCACATTCTCGCTGGCGTGCCGATTGTTCGTGAGCGTAGACGATGAAATCCAGGTGGAGAGATTATCGAGACCGTTTGAGCACATTGTGGCAGGAATCATATCGTTGCCGATCGATTTACCAGGAACGCCGTTCAATCGAGCAATAAAGGCATCGAATTTCATCAGAAAGGAACTGGTGGCGATCGTGAGGCAGAGGAAATGGGATCTGGCGGAAGGAAAGGCGTCGCCGACGCAGGACATTCTGTCTCACATGCTTCTCACGTGCGATGAGAACGGATTTTACATGAACGAGTCCGATATCGCCGATAAGATTCTGGGGCTGTTGATCGGCGGCCATGACACTACCAGTGTCGCATGCACCTTCATCCTTAAGTTCCTTGCTGAGCTTCCTCATGTGTACGATGGCGTCTACGCGGGTAAGCTTAATATATATGAAAAGACTAATACATCCCTCTCCATATACATCCTCCTCAAAATAATATCTTAAAATTTTCTTTCTTTACAAAAAGAAAGAAAAGAAAAGAAAAGAAAATAGTAAACTTCAAAAATCACCCCTAAACTTTTAGGGGTAGTTGTAATTATATCTTCAAATCGAGTCTTAAACAAAGTATTAAAATTCAACCATCAAACTTAGATAATTGTAGAGATTGTAACAATGGTATTGGTTCGAGGAGTTAATTTTATCATTTGAGAGTCCAATTTTTACAACCGTAAATTTTAAAGTCTAATTTTAACATTTAAAGAAGTTTGAAGACCCAATTTAATATAATTGAAAGTTTGAGAATGTAATTACAATTACCATTATAATTCAAGGATGATTTTTGCATTTTGCCCAAACAATTACATGCTAAACTTAAGATTATGAATTATGTGGACACACCTGGTAGTTGCAATAAAGATGCACCAAATGAAAAGTAATATGGTGTATTCCTATCTACACCTAGGCCACTAATAATTCAATCACAATTTATCATGTGACAAACTCTTTTTCTTTTTTTAAAACAATTGATTTATGTGTAATTGATTTGGAAGATGGATGCAACCTTAGCTAGAGAAGTATTACTTGAGCATTGATTTTACAAAATTTTCAAATCACATTTACTTATTTAGATTAGAAAAATAGTTTGTTGAATACATAATTATAAGGGTGTTAAAAAAACTAGATGACCCAACTCAACCAGTACAATTTGGGTTGGGTTATAAACTCATTTGGGTTGGGTTGAGTTCAAATAATTAAAAATTGGTTAAAATACTATTTTAATCCCTATATTTTGTAATTTGTTAAATTTTAGTCCATATACTTTCAAATGTATAATTTTAGTCTCTATACTTTCAATAAATCTTAAATGTAGTCTCTCGAAGTTAAATTTTATCGAAATTGATTAAATAATTACAATATCTTTCATGCAAAGAAAAACAATATGTGAATACGTTCTCAAAATTTATAGTGAATATGCTAATAAAAAATAAAAGGTAATGAAAAAATTCACAACCAACTAGTAGGAGAGACTAAATTTAAGATTTATTGAAAGTACAGATACTAAAATTGAACGTTTGAAAGTAGGCTAAAATTAAACAAACTCCAAAGTATAGAGACCAAAATGGTATTTTTCACCATAAAAATTTTATGGGTTGTATTGGTTATGGATTCACTTAAAATAACTCAAACCAACTCAAACCAACCTGAACTTCTTAATAATTATAAAAAGTACGTCTTTTACTTATGATAATATATTTATATATGCATTATATTTATTTTTATTTTATTAATTTTTTGGATCAATAATTCTCCAAAAACTTCTAAAAATAATTGTAACGACTTTGTAAAGAAAAAAATTCATAATATAATTTGAAATTGAGTCTTAATCTTAATCCAATATATAGAAATAATTAAATAAAACTTTTACATATTAATATTTTATTTCTTACTAGACTAAAAATTTAAATAAATGACTTGAGTAGCCTAAACCAACCAAACCCAAATGTATCGTGGTTGGGTTGGATTAGGTTCGGGTTAGGTTAGGTTTATTATTTAATAAGAGTTATTTGGGTTGAAAATCCTTACAACCCGAATAATTGAGTTGGGTTTATAAGGTACTTCCACCCAACCCAACCCAACCCATGAACGCCCCTACCTATTTTTGTATAGTTCATTCAATAATTTTATCATAGTTTGTCAAGTGACAAATTTTTTTTTTTAGAAAAATTATTTTAAATTTTATTTTAATCATAGTTTGCCAACTAAATATTTCTATGTGATGGGAAGAGTTCTCTATGTAATGCTGTTTAAATTAAGAAAAACAAATTTTTAGTTTGAGTGTGGAGACTACCTCTTGTTCACGTATATAGCTCACTCGAAAAGTTGAACCGTTTTTAAAAGTTGAACAGTTTTTAATCTCTCTACGTCTCAGTTATATATATGTATAACATGGTAATGGTAGAAAATTAAAGTGAGGGAAACAAAAATTTTAGGTTAAAATACTAGTGAATCACTAGTTTTACTTTATTTGTTGATTTTATTTTTTAAAAAACTAATCGCTTTATAAATTATAAATATATATTCTGGTTTTGATATTAAAATTATTATTATTATTTACTAATTTTATTAAATTTAAGATTTATGAATATATAAGAACTAACTTAAAATTGATTGAAAATATTGAAACTGTAAAATTTAACCATTAAAAATATAGAGACCAAAATAAAATTGATCTCGGAGTAGATGTACTTTTGAGTCTTTTACTTCAAGTTTATCAATTTTAGTTATGTAATTTTAAATATTTAATTTTAGTTTTTATATTTTTAATAAATTTTGAAATGGTTGTAATTTTTTTTAAAAAGAAAAAGTTTCAATGGCATTTTTCTCTTATAAATTTGGAAATATATAGAAAATTATTATTATTATTTTACTGATTTGATGAAAGTAAACTAGAAGGAAAACATTTTAAGATTATTGAAAATGCACGCACTAAAATCCAACCACTGTAATTATATCAACTAAAATAAATAAAATTGAAATTGTAAAGCAGATTATGTAACCTAAGAAATTTATATAATAAAAATAATATTTAAATTTTCTCCTTCACTTTTCTTTTTATATTTCTAATATCATATATATTTGTGTAGGTGAAATCTTAAAGTTTACTCGACAAGTTTAGTATCACAATATTTTTAGTTGGCGTATATTTAGTAATAAATTTTTAAATTTTTTTAATAAAATATGAATTTTCAATTTTATGTTCAATAAATCTTTAAATTTAAGGAATATCTAGTAAGTTCCTAATTATTCAATTTCATGTCCAATAAATCTCTAAACCTTAAAAAATATCTTATTTTATTTTATTTTATGTAAGTATATAACCTATTTAAAGATTTTTTATCCCACCTATATATATAACTATAAAGATTTTATAGTTTTGTTTTCCTAATTCGTTCTTTGTTTTTAATGACAAAACTGCTGTAAATATTTTCAAGCATAACACAATGTTATTGACACTAATAGATAGTGACATTTTACTATATTTATAAATATTTTAGCTCATTTTGCTATATTAGAATTAGTTTATTAAAAAATAATAGTAATAAAATTAATGATGAAAATGATATTACTAATGAACTAATTTTTATAATTTAATTTTTCTTTAATTGTTTTATTTTTAAGTATTGCATTTGGAAAGAAGTATTTTGTTTCATAAAAATAGAAAACCAAAACCAAAAAAAAAAAAAAAAAAGTCATGTGATAAATTGTAGTTAGAACAATTAAATAAAAGTGTAGGATACGTCTAATTATTTTTCTCAGTATAATTTTTTTATATCAAAATAATTCCATAACCAAAATGATAATATTATGATAACTTTTTTTTGCAAATTGATTAACAAATTAATTTGCAAAATTTGAATATGAACAGAGCAAATGGAAATAGCAAAAGCAAAAGCCCCAGGGGAAGCATTGAAGTGGGAAGACATAAACAAGATGAAATATTCATGGAATGTGGCCTGTGAGGTTCTAAGAATTGCTTCCCCAGTCCAAGGTGCCTTTAGGGAAGCCTTAAACGACTTCGTTTTTAATGGCTTCTACATTCCCAAGGGCTGGAAGGTAATTTTCTCTCCTCCCTCCTTTACCTTAATATATATATAA

mRNA sequence

ATGAAAAACCACCTAAGGGCTTGTTTGGTTAACAATTCGGATTTTGTGATAAATAGTTTTCTTAAGAAGAGCTCACGTATTTGTGCCTTTATATTTCCCACAAAAATGACCAATCTCCCTCCAACCTTCTTCCTCTCTGCAGTTCAAAACCTCTTTCTCAACGTAACAATGGAGCATTTCTTAATCTCTCTCTTAATCCTCTTCCTCTTTCTCCTCTCTCTCACTTTCTTCATCCTCTTCCACAAGCACAAATCCTTGTTCTCCTATCCCAACGTTCCTCCCGGCGCCATAGGCTTTCCCATTCTTGGTGAGAGCCTCGAGTTCTTGTCCACTGGCTGGAAAGGCCACCCTGAGAAGTTCATCTTCGACCGTTTGAATAAGTACAAGTCTGATGTCTTCAAAACCTCCATCGTCGGCGTTCCGGCCGCCATTTTCTGCGGCCCTGCCTGCAACAAGTTCCTCTTCTCTAACGAGAATAAACTCGTTACTTCTTGGTGGCCGGATTCCGTTAACAAGATCTTTCCCTCTTCCACTCAGACTAACTCCAAAGAGGAAGCTAAGAAACTCAAGAAATTCCTCCCGCAATTCCTTAAGCCGGAAGCGCTTCAGCGTTATATTGGAATTATGGATGAACTCGCTCAACGCCATTTCGCTTCCTTCTGGGAGAACAGGGAAGAGGTCCTTGTGTTTCCTCTCGCTAAAAGTTTAATCGAAATTCAATTTCTCCAATCTTTGATTTTGGTTGATCGACTTCCGATAATGGAATTAGTTTCATGTTCTCGTTCGATTTCGATTTCTGCTTCTTCACAATCTCGTTCGATTTCGTTTTCCGTTTTAATTTTTCTTAAACTGATTTCCGATGAAATTAGGTTCACATTCTCGCTGGCGTGCCGATTGTTCGTGAGCGTAGACGATGAAATCCAGGTGGAGAGATTATCGAGACCGTTTGAGCACATTGTGGCAGGAATCATATCGTTGCCGATCGATTTACCAGGAACGCCGTTCAATCGAGCAATAAAGGCATCGAATTTCATCAGAAAGGAACTGGTGGCGATCGTGAGGCAGAGGAAATGGGATCTGGCGGAAGGAAAGGCGTCGCCGACGCAGGACATTCTGTCTCACATGCTTCTCACGTGCGATGAGAACGGATTTTACATGAACGAGTCCGATATCGCCGATAAGATTCTGGGGCTGTTGATCGGCGGCCATGACACTACCAGTGTCGCATGCACCTTCATCCTTAAGTTCCTTGCTGAGCTTCCTCATGTGTACGATGGCGTCTACGCGGAGCAAATGGAAATAGCAAAAGCAAAAGCCCCAGGGGAAGCATTGAAGTGGGAAGACATAAACAAGATGAAATATTCATGGAATGTGGCCTGTGAGGTTCTAAGAATTGCTTCCCCAGTCCAAGGTGCCTTTAGGGAAGCCTTAAACGACTTCGTTTTTAATGGCTTCTACATTCCCAAGGGCTGGAAGGTAATTTTCTCTCCTCCCTCCTTTACCTTAATATATATATAA

Coding sequence (CDS)

ATGAAAAACCACCTAAGGGCTTGTTTGGTTAACAATTCGGATTTTGTGATAAATAGTTTTCTTAAGAAGAGCTCACGTATTTGTGCCTTTATATTTCCCACAAAAATGACCAATCTCCCTCCAACCTTCTTCCTCTCTGCAGTTCAAAACCTCTTTCTCAACGTAACAATGGAGCATTTCTTAATCTCTCTCTTAATCCTCTTCCTCTTTCTCCTCTCTCTCACTTTCTTCATCCTCTTCCACAAGCACAAATCCTTGTTCTCCTATCCCAACGTTCCTCCCGGCGCCATAGGCTTTCCCATTCTTGGTGAGAGCCTCGAGTTCTTGTCCACTGGCTGGAAAGGCCACCCTGAGAAGTTCATCTTCGACCGTTTGAATAAGTACAAGTCTGATGTCTTCAAAACCTCCATCGTCGGCGTTCCGGCCGCCATTTTCTGCGGCCCTGCCTGCAACAAGTTCCTCTTCTCTAACGAGAATAAACTCGTTACTTCTTGGTGGCCGGATTCCGTTAACAAGATCTTTCCCTCTTCCACTCAGACTAACTCCAAAGAGGAAGCTAAGAAACTCAAGAAATTCCTCCCGCAATTCCTTAAGCCGGAAGCGCTTCAGCGTTATATTGGAATTATGGATGAACTCGCTCAACGCCATTTCGCTTCCTTCTGGGAGAACAGGGAAGAGGTCCTTGTGTTTCCTCTCGCTAAAAGTTTAATCGAAATTCAATTTCTCCAATCTTTGATTTTGGTTGATCGACTTCCGATAATGGAATTAGTTTCATGTTCTCGTTCGATTTCGATTTCTGCTTCTTCACAATCTCGTTCGATTTCGTTTTCCGTTTTAATTTTTCTTAAACTGATTTCCGATGAAATTAGGTTCACATTCTCGCTGGCGTGCCGATTGTTCGTGAGCGTAGACGATGAAATCCAGGTGGAGAGATTATCGAGACCGTTTGAGCACATTGTGGCAGGAATCATATCGTTGCCGATCGATTTACCAGGAACGCCGTTCAATCGAGCAATAAAGGCATCGAATTTCATCAGAAAGGAACTGGTGGCGATCGTGAGGCAGAGGAAATGGGATCTGGCGGAAGGAAAGGCGTCGCCGACGCAGGACATTCTGTCTCACATGCTTCTCACGTGCGATGAGAACGGATTTTACATGAACGAGTCCGATATCGCCGATAAGATTCTGGGGCTGTTGATCGGCGGCCATGACACTACCAGTGTCGCATGCACCTTCATCCTTAAGTTCCTTGCTGAGCTTCCTCATGTGTACGATGGCGTCTACGCGGAGCAAATGGAAATAGCAAAAGCAAAAGCCCCAGGGGAAGCATTGAAGTGGGAAGACATAAACAAGATGAAATATTCATGGAATGTGGCCTGTGAGGTTCTAAGAATTGCTTCCCCAGTCCAAGGTGCCTTTAGGGAAGCCTTAAACGACTTCGTTTTTAATGGCTTCTACATTCCCAAGGGCTGGAAGGTAATTTTCTCTCCTCCCTCCTTTACCTTAATATATATATAA

Protein sequence

MKNHLRACLVNNSDFVINSFLKKSSRICAFIFPTKMTNLPPTFFLSAVQNLFLNVTMEHFLISLLILFLFLLSLTFFILFHKHKSLFSYPNVPPGAIGFPILGESLEFLSTGWKGHPEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPSSTQTNSKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVFPLAKSLIEIQFLQSLILVDRLPIMELVSCSRSISISASSQSRSISFSVLIFLKLISDEIRFTFSLACRLFVSVDDEIQVERLSRPFEHIVAGIISLPIDLPGTPFNRAIKASNFIRKELVAIVRQRKWDLAEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVACTFILKFLAELPHVYDGVYAEQMEIAKAKAPGEALKWEDINKMKYSWNVACEVLRIASPVQGAFREALNDFVFNGFYIPKGWKVIFSPPSFTLIYI
Homology
BLAST of CaUC03G061480 vs. NCBI nr
Match: XP_038877759.1 (LOW QUALITY PROTEIN: beta-amyrin 28-monooxygenase-like [Benincasa hispida])

HSP 1 Score: 689.1 bits (1777), Expect = 2.8e-194
Identity = 348/443 (78.56%), Postives = 363/443 (81.94%), Query Frame = 0

Query: 57  MEHFLISLLILFLFLLSLTFFILFHKHKSLFSYPNVPPGAIGFPILGESLEFLSTGWKGH 116
           MEHFLISLLI FLF LSLT FILFH HKSLFSYPNVPPGAIG PILGESLEFLSTGWKG 
Sbjct: 1   MEHFLISLLIFFLFFLSLTLFILFHNHKSLFSYPNVPPGAIGLPILGESLEFLSTGWKGE 60

Query: 117 PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPS 176
           PEKFIFDRL+KYKSDVFKTSIVGVPAAIFCG ACNKFLFSNENKLVT WWPDSVNKIFPS
Sbjct: 61  PEKFIFDRLHKYKSDVFKTSIVGVPAAIFCGSACNKFLFSNENKLVTPWWPDSVNKIFPS 120

Query: 177 STQTNSKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVFPLAKSL 236
           STQT+SKEEAKKLKKFLPQFLKPEALQRYIG+MDELAQRHFASFWENREEVLVFPLAKS 
Sbjct: 121 STQTSSKEEAKKLKKFLPQFLKPEALQRYIGVMDELAQRHFASFWENREEVLVFPLAKS- 180

Query: 237 IEIQFLQSLILVDRLPIMELVSCSRSISISASSQSRSISFSVLIFLKLISDEIRFTFSLA 296
                                                                 FTFSLA
Sbjct: 181 ------------------------------------------------------FTFSLA 240

Query: 297 CRLFVSVDDEIQVERLSRPFEHIVAGIISLPIDLPGTPFNRAIKASNFIRKELVAIVRQR 356
           CRLFVSV+DEI V+RLS PFEHI AGIIS+PIDLPGTPFNRAIKAS FIRKELVAIV++R
Sbjct: 241 CRLFVSVEDEIHVKRLSGPFEHIAAGIISVPIDLPGTPFNRAIKASKFIRKELVAIVKKR 300

Query: 357 KWDLAEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVACTFILKF 416
           +WDLAEGKAS TQDILSHMLLTCDENG +MNESDIADKILGLLIGGHDT SVACTFI+KF
Sbjct: 301 RWDLAEGKASATQDILSHMLLTCDENGVFMNESDIADKILGLLIGGHDTASVACTFIVKF 360

Query: 417 LAELPHVYDGVYAEQMEIAKAKAPGEALKWEDINKMKYSWNVACEVLRIASPVQGAFREA 476
           LAELPH+Y GVY EQMEIAK KAPGEALKWEDI KMKYSWNV CEVLR+ASP QGAFREA
Sbjct: 361 LAELPHIYHGVYTEQMEIAKGKAPGEALKWEDIKKMKYSWNVVCEVLRLASPXQGAFREA 388

Query: 477 LNDFVFNGFYIPKGWKVIFSPPS 500
           L DFVFNGFYIPKGWK+ +S  S
Sbjct: 421 LTDFVFNGFYIPKGWKLYWSANS 388

BLAST of CaUC03G061480 vs. NCBI nr
Match: XP_008443638.1 (PREDICTED: beta-amyrin 28-oxidase [Cucumis melo])

HSP 1 Score: 676.0 bits (1743), Expect = 2.5e-190
Identity = 341/443 (76.98%), Postives = 359/443 (81.04%), Query Frame = 0

Query: 57  MEHFLISLLILFLFLLSLTFFILFHKHKSLFSYPNVPPGAIGFPILGESLEFLSTGWKGH 116
           MEHFLISLLIL  F LSLT FILFH HKSLFSYPN PPGAIG PILGESLEFLS+GW+GH
Sbjct: 1   MEHFLISLLILLFFFLSLTLFILFHNHKSLFSYPNTPPGAIGLPILGESLEFLSSGWRGH 60

Query: 117 PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPS 176
           PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGP CNKFLFSNENKLVT WWPDSVNKIFPS
Sbjct: 61  PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNKIFPS 120

Query: 177 STQTNSKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVFPLAKSL 236
           +TQT+SKEEAKKLKK LPQFLKPEALQRYIGIMDELA+RHF SFW+NREEVLVFPLAKS 
Sbjct: 121 TTQTSSKEEAKKLKKLLPQFLKPEALQRYIGIMDELAERHFNSFWKNREEVLVFPLAKS- 180

Query: 237 IEIQFLQSLILVDRLPIMELVSCSRSISISASSQSRSISFSVLIFLKLISDEIRFTFSLA 296
                                                                 FTFSLA
Sbjct: 181 ------------------------------------------------------FTFSLA 240

Query: 297 CRLFVSVDDEIQVERLSRPFEHIVAGIISLPIDLPGTPFNRAIKASNFIRKELVAIVRQR 356
           CRLF+SV+DEI VERLS PFEHI AGIIS+PIDLPGTPFNRAIKAS FIRKE+VAIVRQR
Sbjct: 241 CRLFMSVEDEIHVERLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEVVAIVRQR 300

Query: 357 KWDLAEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVACTFILKF 416
           K DLAEGKA  TQDILSHMLLTCDENG YMNESDI DKILGLLIGGHDT SVACTFI+KF
Sbjct: 301 KQDLAEGKALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVACTFIVKF 360

Query: 417 LAELPHVYDGVYAEQMEIAKAKAPGEALKWEDINKMKYSWNVACEVLRIASPVQGAFREA 476
           LAELPH+YD VY EQMEIA+AKA GE LKWEDI KMKYSWNVACEVLR+ASP+QGAFREA
Sbjct: 361 LAELPHIYDAVYTEQMEIARAKAEGETLKWEDIKKMKYSWNVACEVLRLASPLQGAFREA 388

Query: 477 LNDFVFNGFYIPKGWKVIFSPPS 500
           LNDFVFNGF+IPKGWK+ +S  S
Sbjct: 421 LNDFVFNGFFIPKGWKLYWSANS 388

BLAST of CaUC03G061480 vs. NCBI nr
Match: XP_004139087.1 (beta-amyrin 28-monooxygenase [Cucumis sativus] >KGN66615.1 hypothetical protein Csa_007564 [Cucumis sativus])

HSP 1 Score: 668.7 bits (1724), Expect = 4.0e-188
Identity = 338/443 (76.30%), Postives = 358/443 (80.81%), Query Frame = 0

Query: 57  MEHFLISLLILFLFLLSLTFFILFHKHKSLFSYPNVPPGAIGFPILGESLEFLSTGWKGH 116
           ME FLISLLIL  F LSLT FILFH HKSLFSYPN PPGAIG PILGES+EFLS+GWKGH
Sbjct: 1   MELFLISLLILLFFFLSLTLFILFHNHKSLFSYPNTPPGAIGLPILGESVEFLSSGWKGH 60

Query: 117 PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPS 176
           PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGP CNKFLFSNENKLVT WWPDSVNKIFPS
Sbjct: 61  PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNKIFPS 120

Query: 177 STQTNSKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVFPLAKSL 236
           +TQT++KEEAKKLKK LPQFLKPEALQRYIGIMDELA+RHF SFW+NREEVLVFPLAKS 
Sbjct: 121 TTQTSTKEEAKKLKKLLPQFLKPEALQRYIGIMDELAERHFNSFWKNREEVLVFPLAKS- 180

Query: 237 IEIQFLQSLILVDRLPIMELVSCSRSISISASSQSRSISFSVLIFLKLISDEIRFTFSLA 296
                                                                 FTFS+A
Sbjct: 181 ------------------------------------------------------FTFSIA 240

Query: 297 CRLFVSVDDEIQVERLSRPFEHIVAGIISLPIDLPGTPFNRAIKASNFIRKELVAIVRQR 356
           CRLF+SV+DEI VERLS PFEHI AGIIS+PIDLPGTPFNRAIKAS FIRKE+VAIVRQR
Sbjct: 241 CRLFMSVEDEIHVERLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEVVAIVRQR 300

Query: 357 KWDLAEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVACTFILKF 416
           K DLAEGKA  TQDILSHMLLTCDENG YMNESDI DKILGLLIGGHDT SVACTFI+KF
Sbjct: 301 KQDLAEGKALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVACTFIVKF 360

Query: 417 LAELPHVYDGVYAEQMEIAKAKAPGEALKWEDINKMKYSWNVACEVLRIASPVQGAFREA 476
           LAELPH+YD VY EQMEIA+AKA GE LKWEDI KMKYSWNVACEVLRIASP+QGAFREA
Sbjct: 361 LAELPHIYDAVYTEQMEIARAKAEGETLKWEDIKKMKYSWNVACEVLRIASPLQGAFREA 388

Query: 477 LNDFVFNGFYIPKGWKVIFSPPS 500
           L+DFVFNGF+IPKGWK+ +S  S
Sbjct: 421 LSDFVFNGFFIPKGWKLYWSANS 388

BLAST of CaUC03G061480 vs. NCBI nr
Match: KAG6590124.1 (Beta-amyrin 28-monooxygenase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7023791.1 hypothetical protein SDJN02_14817 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 653.3 bits (1684), Expect = 1.7e-183
Identity = 330/443 (74.49%), Postives = 355/443 (80.14%), Query Frame = 0

Query: 57  MEHFLISLLILFLFLLSLTFFILFHKHKSLFSYPNVPPGAIGFPILGESLEFLSTGWKGH 116
           M  FLISLLILFL L+SLT F+LF+KHKSLFSYPN PPGAIG PI+GESLEFLSTGWKG 
Sbjct: 1   MADFLISLLILFLSLISLTLFVLFYKHKSLFSYPNTPPGAIGLPIIGESLEFLSTGWKGE 60

Query: 117 PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPS 176
           PEKFIFDRLNKYKSD+FKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFP+
Sbjct: 61  PEKFIFDRLNKYKSDIFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPA 120

Query: 177 STQTNSKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVFPLAKSL 236
           ST+ NSKE A +LKK LP+FLKPEALQRYIG+MDELAQRHFAS WENRE+VLVFPLAKS 
Sbjct: 121 STEINSKESAIRLKKLLPRFLKPEALQRYIGVMDELAQRHFASSWENREQVLVFPLAKS- 180

Query: 237 IEIQFLQSLILVDRLPIMELVSCSRSISISASSQSRSISFSVLIFLKLISDEIRFTFSLA 296
                                                                 FTFSLA
Sbjct: 181 ------------------------------------------------------FTFSLA 240

Query: 297 CRLFVSVDDEIQVERLSRPFEHIVAGIISLPIDLPGTPFNRAIKASNFIRKELVAIVRQR 356
           CRLFVS++D I VERLS PF+ I AGIIS+PIDLPGTPFNRAIKASNF+RKEL++IVRQR
Sbjct: 241 CRLFVSLEDRIHVERLSEPFQSIAAGIISMPIDLPGTPFNRAIKASNFVRKELLSIVRQR 300

Query: 357 KWDLAEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVACTFILKF 416
           K DLAEGKASPTQDILSHMLLTCDE G YMNES IAD ILGLLIGGHDTTS ACTFI+K+
Sbjct: 301 KSDLAEGKASPTQDILSHMLLTCDEIGQYMNESAIADNILGLLIGGHDTTSAACTFIVKY 360

Query: 417 LAELPHVYDGVYAEQMEIAKAKAPGEALKWEDINKMKYSWNVACEVLRIASPVQGAFREA 476
           LAELP +YDGVY EQMEIAKAKA GE LKWEDI KMKYSWNV CEVLR+ASP+QGAFREA
Sbjct: 361 LAELPQIYDGVYREQMEIAKAKAAGELLKWEDIQKMKYSWNVGCEVLRLASPLQGAFREA 388

Query: 477 LNDFVFNGFYIPKGWKVIFSPPS 500
           +N FVFNGFYIPKGWKV +S  S
Sbjct: 421 INHFVFNGFYIPKGWKVYWSSNS 388

BLAST of CaUC03G061480 vs. NCBI nr
Match: XP_022960871.1 (beta-amyrin 28-oxidase [Cucurbita moschata])

HSP 1 Score: 652.9 bits (1683), Expect = 2.3e-183
Identity = 330/443 (74.49%), Postives = 355/443 (80.14%), Query Frame = 0

Query: 57  MEHFLISLLILFLFLLSLTFFILFHKHKSLFSYPNVPPGAIGFPILGESLEFLSTGWKGH 116
           M  FLISLLILFL L+SLT F+LF+KHKSLFSYPN PPGAIG PI+GESLEFLSTGWKG 
Sbjct: 1   MADFLISLLILFLSLISLTLFVLFYKHKSLFSYPNTPPGAIGLPIIGESLEFLSTGWKGE 60

Query: 117 PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPS 176
           PEKFIFDRLNKYKSD+FKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFP+
Sbjct: 61  PEKFIFDRLNKYKSDIFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPA 120

Query: 177 STQTNSKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVFPLAKSL 236
           ST+ NSKE A +LKK LP+FLKPEALQRYIG+MDELAQRHFAS WENRE+VLVFPLAKS 
Sbjct: 121 STEINSKESAIRLKKLLPRFLKPEALQRYIGVMDELAQRHFASSWENREQVLVFPLAKS- 180

Query: 237 IEIQFLQSLILVDRLPIMELVSCSRSISISASSQSRSISFSVLIFLKLISDEIRFTFSLA 296
                                                                 FTFSLA
Sbjct: 181 ------------------------------------------------------FTFSLA 240

Query: 297 CRLFVSVDDEIQVERLSRPFEHIVAGIISLPIDLPGTPFNRAIKASNFIRKELVAIVRQR 356
           CRLFVS++D I VERLS PF+ I AGIIS+PIDLPGTPFNRAIKASNF+RKEL++IVRQR
Sbjct: 241 CRLFVSLEDRIHVERLSEPFQSIAAGIISMPIDLPGTPFNRAIKASNFVRKELLSIVRQR 300

Query: 357 KWDLAEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVACTFILKF 416
           K DLAEGKASPTQDILSHMLLTCDE G YMNES IAD ILGLLIGGHDTTS ACTFI+ +
Sbjct: 301 KSDLAEGKASPTQDILSHMLLTCDEIGQYMNESAIADNILGLLIGGHDTTSAACTFIVNY 360

Query: 417 LAELPHVYDGVYAEQMEIAKAKAPGEALKWEDINKMKYSWNVACEVLRIASPVQGAFREA 476
           LAELP +YDGVY EQMEIAKAKA GE LKWEDI KMKYSWNVACEVLR+ASP+QGAFREA
Sbjct: 361 LAELPQIYDGVYREQMEIAKAKAAGELLKWEDIQKMKYSWNVACEVLRLASPLQGAFREA 388

Query: 477 LNDFVFNGFYIPKGWKVIFSPPS 500
           +N FVFNGFYIPKGWKV +S  S
Sbjct: 421 INHFVFNGFYIPKGWKVYWSSNS 388

BLAST of CaUC03G061480 vs. ExPASy Swiss-Prot
Match: F6H9N6 (Beta-amyrin 28-monooxygenase OS=Vitis vinifera OX=29760 GN=CYP716A15 PE=1 SV=1)

HSP 1 Score: 556.2 bits (1432), Expect = 3.8e-157
Identity = 271/443 (61.17%), Postives = 333/443 (75.17%), Query Frame = 0

Query: 57  MEHFLISLLILFLFLLSLTFFILFHKHKSLFSYPNVPPGAIGFPILGESLEFLSTGWKGH 116
           ME F +SLL++F+  +S+   +LF+KH+S F+ PN+PPG IG+P++GESLEFLSTGWKGH
Sbjct: 1   MEVFFLSLLLIFVLSVSIGLHLLFYKHRSHFTGPNLPPGKIGWPMVGESLEFLSTGWKGH 60

Query: 117 PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPS 176
           PEKFIFDR++KY S+VFKTS++G PAA+F G A NKFLFSNENKLV +WWP SV+K+FPS
Sbjct: 61  PEKFIFDRISKYSSEVFKTSLLGEPAAVFAGAAGNKFLFSNENKLVHAWWPSSVDKVFPS 120

Query: 177 STQTNSKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVFPLAKSL 236
           STQT+SKEEAKK++K LPQF KPEALQRYIGIMD +AQRHFA  W+NR+EV+VFPLAK  
Sbjct: 121 STQTSSKEEAKKMRKLLPQFFKPEALQRYIGIMDHIAQRHFADSWDNRDEVIVFPLAK-- 180

Query: 237 IEIQFLQSLILVDRLPIMELVSCSRSISISASSQSRSISFSVLIFLKLISDEIRFTFSLA 296
                                                                RFTF LA
Sbjct: 181 -----------------------------------------------------RFTFWLA 240

Query: 297 CRLFVSVDDEIQVERLSRPFEHIVAGIISLPIDLPGTPFNRAIKASNFIRKELVAIVRQR 356
           CRLF+S++D   V +  +PF  + +G+I++PIDLPGTPF+RAIKASNFIRKEL AI++QR
Sbjct: 241 CRLFMSIEDPAHVAKFEKPFHVLASGLITVPIDLPGTPFHRAIKASNFIRKELRAIIKQR 300

Query: 357 KWDLAEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVACTFILKF 416
           K DLAEGKAS  QDILSHMLL  DE+G +MNE +IADKILGLLIGGHDT S A TF++K+
Sbjct: 301 KIDLAEGKASQNQDILSHMLLATDEDGCHMNEMEIADKILGLLIGGHDTASAAITFLIKY 360

Query: 417 LAELPHVYDGVYAEQMEIAKAKAPGEALKWEDINKMKYSWNVACEVLRIASPVQGAFREA 476
           +AELPH+Y+ VY EQMEIA +KAPGE L W+D+ KM+YSWNVACEV+R+A P+QGAFREA
Sbjct: 361 MAELPHIYEKVYEEQMEIANSKAPGELLNWDDVQKMRYSWNVACEVMRLAPPLQGAFREA 388

Query: 477 LNDFVFNGFYIPKGWKVIFSPPS 500
           + DFVFNGF IPKGWK+ +S  S
Sbjct: 421 ITDFVFNGFSIPKGWKLYWSANS 388

BLAST of CaUC03G061480 vs. ExPASy Swiss-Prot
Match: A5BFI4 (Beta-amyrin 28-monooxygenase OS=Vitis vinifera OX=29760 GN=CYP716A17 PE=1 SV=2)

HSP 1 Score: 550.4 bits (1417), Expect = 2.1e-155
Identity = 269/443 (60.72%), Postives = 331/443 (74.72%), Query Frame = 0

Query: 57  MEHFLISLLILFLFLLSLTFFILFHKHKSLFSYPNVPPGAIGFPILGESLEFLSTGWKGH 116
           ME F +SLL++ +  +S+  ++L +KH+S F+ PN+PPG IG+P++GESLEFLSTGWKGH
Sbjct: 1   MEVFFLSLLLICVLSVSIRLYLLLYKHRSHFTGPNLPPGKIGWPMVGESLEFLSTGWKGH 60

Query: 117 PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPS 176
           PEKFIFDR++KY S+VFKTS++G PAA+F G A NKFLFSNENKLV +WWP SV+K+FPS
Sbjct: 61  PEKFIFDRISKYSSEVFKTSLLGEPAAVFAGAAGNKFLFSNENKLVHAWWPSSVDKVFPS 120

Query: 177 STQTNSKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVFPLAKSL 236
           STQT+SKEEAKK++K LPQFLKPEALQRY GIMD +AQRHFA  W+NR+EV+VFPLAK  
Sbjct: 121 STQTSSKEEAKKMRKLLPQFLKPEALQRYTGIMDHIAQRHFADSWDNRDEVIVFPLAK-- 180

Query: 237 IEIQFLQSLILVDRLPIMELVSCSRSISISASSQSRSISFSVLIFLKLISDEIRFTFSLA 296
                                                                RFTF LA
Sbjct: 181 -----------------------------------------------------RFTFWLA 240

Query: 297 CRLFVSVDDEIQVERLSRPFEHIVAGIISLPIDLPGTPFNRAIKASNFIRKELVAIVRQR 356
           CRLF+S++D   V +  +PF  + +G+I++PIDLPGTPF+RAIKASNFIRKEL AI++QR
Sbjct: 241 CRLFMSIEDPAHVAKFEKPFHVLASGLITIPIDLPGTPFHRAIKASNFIRKELRAIIKQR 300

Query: 357 KWDLAEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVACTFILKF 416
           K DLAE KAS TQDILSHMLL  DE+G +MNE  IADKILGLLIGGHDT S A TF++K+
Sbjct: 301 KIDLAESKASKTQDILSHMLLATDEDGCHMNEMSIADKILGLLIGGHDTASSAITFLVKY 360

Query: 417 LAELPHVYDGVYAEQMEIAKAKAPGEALKWEDINKMKYSWNVACEVLRIASPVQGAFREA 476
           +AELPH+Y+ VY EQMEIA +KAPGE L W+D+ KM+YSWNVACEV+R+A P+QGAFREA
Sbjct: 361 MAELPHIYEKVYKEQMEIANSKAPGELLNWDDVQKMRYSWNVACEVMRLAPPLQGAFREA 388

Query: 477 LNDFVFNGFYIPKGWKVIFSPPS 500
           + DFVFNGF IPKGWK+ +S  S
Sbjct: 421 ITDFVFNGFSIPKGWKLYWSANS 388

BLAST of CaUC03G061480 vs. ExPASy Swiss-Prot
Match: A0A0S2II38 (Beta-amyrin 28-monooxygenase OS=Kalopanax septemlobus OX=228393 GN=CYP716A94 PE=1 SV=1)

HSP 1 Score: 539.3 bits (1388), Expect = 4.8e-152
Identity = 270/444 (60.81%), Postives = 323/444 (72.75%), Query Frame = 0

Query: 57  MEHFLISLLILFLFLLSLTFFILFHKHKS-LFSYPNVPPGAIGFPILGESLEFLSTGWKG 116
           M+ F + LL LF+  +SL+F+ LF+K KS   S   +PPG  G+P++GES EFLSTGWKG
Sbjct: 1   MQLFYVPLLSLFVLFVSLSFYFLFYKSKSGSTSGLPLPPGKTGWPVIGESFEFLSTGWKG 60

Query: 117 HPEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFP 176
           +PEKFIFDRL KY S+VFKTS+ G PAA FCG ACNKFLFSNENKLV +WWPDSVNK+FP
Sbjct: 61  YPEKFIFDRLAKYSSNVFKTSLFGQPAAAFCGAACNKFLFSNENKLVQAWWPDSVNKVFP 120

Query: 177 SSTQTNSKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVFPLAKS 236
           SSTQT+SKEEA K++K LP FLKPEALQRY+G+MD++A +HF S WEN++EVLVFPLAK+
Sbjct: 121 SSTQTSSKEEAIKMRKLLPNFLKPEALQRYVGVMDQIATKHFKSGWENKKEVLVFPLAKN 180

Query: 237 LIEIQFLQSLILVDRLPIMELVSCSRSISISASSQSRSISFSVLIFLKLISDEIRFTFSL 296
                                                                  +TF +
Sbjct: 181 -------------------------------------------------------YTFWI 240

Query: 297 ACRLFVSVDDEIQVERLSRPFEHIVAGIISLPIDLPGTPFNRAIKASNFIRKELVAIVRQ 356
           AC++FVSV++  QV +L  PF  I +GIIS+PIDLPGTPFN AIK+S  +R +L+ I++Q
Sbjct: 241 ACKVFVSVEEPTQVAKLLEPFNAIASGIISVPIDLPGTPFNSAIKSSKIVRDKLMGIIKQ 300

Query: 357 RKWDLAEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVACTFILK 416
           RK DL EGKASPTQDILSHMLLT DENG +M E DIADKILGLLIGGHDT S ACTF++K
Sbjct: 301 RKTDLGEGKASPTQDILSHMLLTSDENGKFMTEGDIADKILGLLIGGHDTASSACTFVVK 360

Query: 417 FLAELPHVYDGVYAEQMEIAKAKAPGEALKWEDINKMKYSWNVACEVLRIASPVQGAFRE 476
           FLAELP +Y+GVY EQMEIAK+K  GE L WEDI KMKYSWNVACEVLR+A P+QGAFRE
Sbjct: 361 FLAELPEIYEGVYKEQMEIAKSKKAGELLNWEDIQKMKYSWNVACEVLRLAPPLQGAFRE 389

Query: 477 ALNDFVFNGFYIPKGWKVIFSPPS 500
           AL DF +NGF IPKGWK+ +S  S
Sbjct: 421 ALTDFSYNGFSIPKGWKLYWSANS 389

BLAST of CaUC03G061480 vs. ExPASy Swiss-Prot
Match: A0A3Q7HBJ5 (Beta-amyrin 28-monooxygenase OS=Solanum lycopersicum OX=4081 GN=CYP716A44 PE=1 SV=1)

HSP 1 Score: 538.1 bits (1385), Expect = 1.1e-151
Identity = 268/443 (60.50%), Postives = 323/443 (72.91%), Query Frame = 0

Query: 57  MEHFLISLLILFLFLLSLTFFILFHKHKSLFSYPNVPPGAIGFPILGESLEFLSTGWKGH 116
           ME   + L+ +F+FL+SL   +L +K K   S   +PPG  G+P+ GESLEFLS+GWKGH
Sbjct: 1   MELLYVCLVCVFVFLVSL---LLLYKKK---SGEGLPPGKTGWPVFGESLEFLSSGWKGH 60

Query: 117 PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPS 176
           PEKFIFDR+ KY S VFKT ++G  AA+FCG + NKFLFSNENKLV +WWP+SVNK+FPS
Sbjct: 61  PEKFIFDRVAKYSSSVFKTHLLGEEAAVFCGASANKFLFSNENKLVQAWWPNSVNKVFPS 120

Query: 177 STQTNSKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVFPLAKSL 236
           STQT+SKEEA K++K LP F KPEALQRY+GIMD + QRHFA+ WEN+E+V+VFPL K  
Sbjct: 121 STQTSSKEEAIKMRKMLPNFFKPEALQRYVGIMDHITQRHFATGWENKEQVVVFPLTK-- 180

Query: 237 IEIQFLQSLILVDRLPIMELVSCSRSISISASSQSRSISFSVLIFLKLISDEIRFTFSLA 296
                                                                R+TF LA
Sbjct: 181 -----------------------------------------------------RYTFWLA 240

Query: 297 CRLFVSVDDEIQVERLSRPFEHIVAGIISLPIDLPGTPFNRAIKASNFIRKELVAIVRQR 356
           CRLF+SV+D   V + + PF+ + +G+IS+PIDLPGTPFNRAIKASNFIRKELV I++QR
Sbjct: 241 CRLFLSVEDPKHVAKFADPFDVLASGLISIPIDLPGTPFNRAIKASNFIRKELVRIIKQR 300

Query: 357 KWDLAEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVACTFILKF 416
           K DL EGK S TQDILSHMLLTCDENG ++ + DIADKILGLLIGGHDT S AC+FI+K+
Sbjct: 301 KIDLGEGKVSSTQDILSHMLLTCDENGKFLGDLDIADKILGLLIGGHDTASSACSFIVKY 360

Query: 417 LAELPHVYDGVYAEQMEIAKAKAPGEALKWEDINKMKYSWNVACEVLRIASPVQGAFREA 476
           LAELPH+Y  VY EQMEIAK+K PGE L+WEDI KMKYSWNVACEVLR+A P+QGAFREA
Sbjct: 361 LAELPHIYQRVYTEQMEIAKSKGPGELLRWEDIQKMKYSWNVACEVLRLAPPLQGAFREA 382

Query: 477 LNDFVFNGFYIPKGWKVIFSPPS 500
           L+DF+FNGFYIPKGWK+ +S  S
Sbjct: 421 LSDFMFNGFYIPKGWKIYWSANS 382

BLAST of CaUC03G061480 vs. ExPASy Swiss-Prot
Match: K4CEE8 (Beta-amyrin 28-monooxygenase OS=Solanum lycopersicum OX=4081 GN=CYP716A46 PE=1 SV=1)

HSP 1 Score: 538.1 bits (1385), Expect = 1.1e-151
Identity = 275/444 (61.94%), Postives = 327/444 (73.65%), Query Frame = 0

Query: 57  MEHFLISLLILFLFLLSLTFFILFH-KHKSLFSYPNVPPGAIGFPILGESLEFLSTGWKG 116
           ME F  SL+ LF+ LLSL+F  LF+ K+KS+     +PPG  G+P++GE+LEFLSTGWKG
Sbjct: 1   MELFYASLVCLFVLLLSLSFLFLFNKKNKSMILSCPLPPGNSGWPVIGETLEFLSTGWKG 60

Query: 117 HPEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFP 176
           HPEKFIFDR++KYKS +FKT ++G  A +FCG + NKFLFSNENKLV +WWP SV+K+FP
Sbjct: 61  HPEKFIFDRISKYKSSIFKTHLLGEKAVVFCGASSNKFLFSNENKLVQTWWPSSVDKVFP 120

Query: 177 SSTQTNSKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVFPLAKS 236
           SSTQT+SKEEA K++K LP FLKPEALQRYI IMD +AQ+HF S WEN++EV+VFPLAK 
Sbjct: 121 SSTQTSSKEEAIKMRKMLPNFLKPEALQRYIRIMDHIAQKHFQS-WENQQEVVVFPLAK- 180

Query: 237 LIEIQFLQSLILVDRLPIMELVSCSRSISISASSQSRSISFSVLIFLKLISDEIRFTFSL 296
                                                                 R+TF L
Sbjct: 181 ------------------------------------------------------RYTFWL 240

Query: 297 ACRLFVSVDDEIQVERLSRPFEHIVAGIISLPIDLPGTPFNRAIKASNFIRKELVAIVRQ 356
           ACRLFVSV+D   V RL+ PF+ + +G+IS+PI+LPGTPFNRAIKASNFIRKELVAI++Q
Sbjct: 241 ACRLFVSVEDPNHVARLADPFDVLASGLISIPINLPGTPFNRAIKASNFIRKELVAIIKQ 300

Query: 357 RKWDLAEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVACTFILK 416
           RK DL EGKAS +QDILSHMLLT DENG +M+E DIADKILGLLIGGHDT S AC FI+K
Sbjct: 301 RKIDLTEGKASDSQDILSHMLLTSDENGKFMHELDIADKILGLLIGGHDTASSACAFIVK 360

Query: 417 FLAELPHVYDGVYAEQMEIAKAKAPGEALKWEDINKMKYSWNVACEVLRIASPVQGAFRE 476
           +LAELP +YD VY EQ+EIAK+K PGE L WEDI KMKYSWNVACEVLR+A P+QGAFRE
Sbjct: 361 YLAELPEIYDQVYKEQIEIAKSKGPGELLSWEDIKKMKYSWNVACEVLRLAPPLQGAFRE 388

Query: 477 ALNDFVFNGFYIPKGWKVIFSPPS 500
           AL DF FNGF IPKGWK+ +S  S
Sbjct: 421 ALVDFTFNGFSIPKGWKIYWSANS 388

BLAST of CaUC03G061480 vs. ExPASy TrEMBL
Match: A0A1S3B8H4 (beta-amyrin 28-oxidase OS=Cucumis melo OX=3656 GN=LOC103487187 PE=3 SV=1)

HSP 1 Score: 676.0 bits (1743), Expect = 1.2e-190
Identity = 341/443 (76.98%), Postives = 359/443 (81.04%), Query Frame = 0

Query: 57  MEHFLISLLILFLFLLSLTFFILFHKHKSLFSYPNVPPGAIGFPILGESLEFLSTGWKGH 116
           MEHFLISLLIL  F LSLT FILFH HKSLFSYPN PPGAIG PILGESLEFLS+GW+GH
Sbjct: 1   MEHFLISLLILLFFFLSLTLFILFHNHKSLFSYPNTPPGAIGLPILGESLEFLSSGWRGH 60

Query: 117 PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPS 176
           PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGP CNKFLFSNENKLVT WWPDSVNKIFPS
Sbjct: 61  PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNKIFPS 120

Query: 177 STQTNSKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVFPLAKSL 236
           +TQT+SKEEAKKLKK LPQFLKPEALQRYIGIMDELA+RHF SFW+NREEVLVFPLAKS 
Sbjct: 121 TTQTSSKEEAKKLKKLLPQFLKPEALQRYIGIMDELAERHFNSFWKNREEVLVFPLAKS- 180

Query: 237 IEIQFLQSLILVDRLPIMELVSCSRSISISASSQSRSISFSVLIFLKLISDEIRFTFSLA 296
                                                                 FTFSLA
Sbjct: 181 ------------------------------------------------------FTFSLA 240

Query: 297 CRLFVSVDDEIQVERLSRPFEHIVAGIISLPIDLPGTPFNRAIKASNFIRKELVAIVRQR 356
           CRLF+SV+DEI VERLS PFEHI AGIIS+PIDLPGTPFNRAIKAS FIRKE+VAIVRQR
Sbjct: 241 CRLFMSVEDEIHVERLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEVVAIVRQR 300

Query: 357 KWDLAEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVACTFILKF 416
           K DLAEGKA  TQDILSHMLLTCDENG YMNESDI DKILGLLIGGHDT SVACTFI+KF
Sbjct: 301 KQDLAEGKALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVACTFIVKF 360

Query: 417 LAELPHVYDGVYAEQMEIAKAKAPGEALKWEDINKMKYSWNVACEVLRIASPVQGAFREA 476
           LAELPH+YD VY EQMEIA+AKA GE LKWEDI KMKYSWNVACEVLR+ASP+QGAFREA
Sbjct: 361 LAELPHIYDAVYTEQMEIARAKAEGETLKWEDIKKMKYSWNVACEVLRLASPLQGAFREA 388

Query: 477 LNDFVFNGFYIPKGWKVIFSPPS 500
           LNDFVFNGF+IPKGWK+ +S  S
Sbjct: 421 LNDFVFNGFFIPKGWKLYWSANS 388

BLAST of CaUC03G061480 vs. ExPASy TrEMBL
Match: A0A0A0LXN3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G649550 PE=3 SV=1)

HSP 1 Score: 668.7 bits (1724), Expect = 1.9e-188
Identity = 338/443 (76.30%), Postives = 358/443 (80.81%), Query Frame = 0

Query: 57  MEHFLISLLILFLFLLSLTFFILFHKHKSLFSYPNVPPGAIGFPILGESLEFLSTGWKGH 116
           ME FLISLLIL  F LSLT FILFH HKSLFSYPN PPGAIG PILGES+EFLS+GWKGH
Sbjct: 1   MELFLISLLILLFFFLSLTLFILFHNHKSLFSYPNTPPGAIGLPILGESVEFLSSGWKGH 60

Query: 117 PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPS 176
           PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGP CNKFLFSNENKLVT WWPDSVNKIFPS
Sbjct: 61  PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPICNKFLFSNENKLVTPWWPDSVNKIFPS 120

Query: 177 STQTNSKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVFPLAKSL 236
           +TQT++KEEAKKLKK LPQFLKPEALQRYIGIMDELA+RHF SFW+NREEVLVFPLAKS 
Sbjct: 121 TTQTSTKEEAKKLKKLLPQFLKPEALQRYIGIMDELAERHFNSFWKNREEVLVFPLAKS- 180

Query: 237 IEIQFLQSLILVDRLPIMELVSCSRSISISASSQSRSISFSVLIFLKLISDEIRFTFSLA 296
                                                                 FTFS+A
Sbjct: 181 ------------------------------------------------------FTFSIA 240

Query: 297 CRLFVSVDDEIQVERLSRPFEHIVAGIISLPIDLPGTPFNRAIKASNFIRKELVAIVRQR 356
           CRLF+SV+DEI VERLS PFEHI AGIIS+PIDLPGTPFNRAIKAS FIRKE+VAIVRQR
Sbjct: 241 CRLFMSVEDEIHVERLSGPFEHIAAGIISMPIDLPGTPFNRAIKASKFIRKEVVAIVRQR 300

Query: 357 KWDLAEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVACTFILKF 416
           K DLAEGKA  TQDILSHMLLTCDENG YMNESDI DKILGLLIGGHDT SVACTFI+KF
Sbjct: 301 KQDLAEGKALATQDILSHMLLTCDENGVYMNESDITDKILGLLIGGHDTASVACTFIVKF 360

Query: 417 LAELPHVYDGVYAEQMEIAKAKAPGEALKWEDINKMKYSWNVACEVLRIASPVQGAFREA 476
           LAELPH+YD VY EQMEIA+AKA GE LKWEDI KMKYSWNVACEVLRIASP+QGAFREA
Sbjct: 361 LAELPHIYDAVYTEQMEIARAKAEGETLKWEDIKKMKYSWNVACEVLRIASPLQGAFREA 388

Query: 477 LNDFVFNGFYIPKGWKVIFSPPS 500
           L+DFVFNGF+IPKGWK+ +S  S
Sbjct: 421 LSDFVFNGFFIPKGWKLYWSANS 388

BLAST of CaUC03G061480 vs. ExPASy TrEMBL
Match: A0A6J1H8T4 (beta-amyrin 28-oxidase OS=Cucurbita moschata OX=3662 GN=LOC111461545 PE=3 SV=1)

HSP 1 Score: 652.9 bits (1683), Expect = 1.1e-183
Identity = 330/443 (74.49%), Postives = 355/443 (80.14%), Query Frame = 0

Query: 57  MEHFLISLLILFLFLLSLTFFILFHKHKSLFSYPNVPPGAIGFPILGESLEFLSTGWKGH 116
           M  FLISLLILFL L+SLT F+LF+KHKSLFSYPN PPGAIG PI+GESLEFLSTGWKG 
Sbjct: 1   MADFLISLLILFLSLISLTLFVLFYKHKSLFSYPNTPPGAIGLPIIGESLEFLSTGWKGE 60

Query: 117 PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPS 176
           PEKFIFDRLNKYKSD+FKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFP+
Sbjct: 61  PEKFIFDRLNKYKSDIFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPA 120

Query: 177 STQTNSKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVFPLAKSL 236
           ST+ NSKE A +LKK LP+FLKPEALQRYIG+MDELAQRHFAS WENRE+VLVFPLAKS 
Sbjct: 121 STEINSKESAIRLKKLLPRFLKPEALQRYIGVMDELAQRHFASSWENREQVLVFPLAKS- 180

Query: 237 IEIQFLQSLILVDRLPIMELVSCSRSISISASSQSRSISFSVLIFLKLISDEIRFTFSLA 296
                                                                 FTFSLA
Sbjct: 181 ------------------------------------------------------FTFSLA 240

Query: 297 CRLFVSVDDEIQVERLSRPFEHIVAGIISLPIDLPGTPFNRAIKASNFIRKELVAIVRQR 356
           CRLFVS++D I VERLS PF+ I AGIIS+PIDLPGTPFNRAIKASNF+RKEL++IVRQR
Sbjct: 241 CRLFVSLEDRIHVERLSEPFQSIAAGIISMPIDLPGTPFNRAIKASNFVRKELLSIVRQR 300

Query: 357 KWDLAEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVACTFILKF 416
           K DLAEGKASPTQDILSHMLLTCDE G YMNES IAD ILGLLIGGHDTTS ACTFI+ +
Sbjct: 301 KSDLAEGKASPTQDILSHMLLTCDEIGQYMNESAIADNILGLLIGGHDTTSAACTFIVNY 360

Query: 417 LAELPHVYDGVYAEQMEIAKAKAPGEALKWEDINKMKYSWNVACEVLRIASPVQGAFREA 476
           LAELP +YDGVY EQMEIAKAKA GE LKWEDI KMKYSWNVACEVLR+ASP+QGAFREA
Sbjct: 361 LAELPQIYDGVYREQMEIAKAKAAGELLKWEDIQKMKYSWNVACEVLRLASPLQGAFREA 388

Query: 477 LNDFVFNGFYIPKGWKVIFSPPS 500
           +N FVFNGFYIPKGWKV +S  S
Sbjct: 421 INHFVFNGFYIPKGWKVYWSSNS 388

BLAST of CaUC03G061480 vs. ExPASy TrEMBL
Match: A0A6J1DZX7 (beta-amyrin 28-oxidase OS=Momordica charantia OX=3673 GN=LOC111024635 PE=3 SV=1)

HSP 1 Score: 649.4 bits (1674), Expect = 1.2e-182
Identity = 322/443 (72.69%), Postives = 351/443 (79.23%), Query Frame = 0

Query: 57  MEHFLISLLILFLFLLSLTFFILFHKHKSLFSYPNVPPGAIGFPILGESLEFLSTGWKGH 116
           MEHFL+SLLI F+ ++ +  FILF+KHKS FSYPN+PPG IGFPI+GESLEF STGWKG 
Sbjct: 1   MEHFLVSLLIFFVSVVCVGLFILFYKHKSPFSYPNIPPGGIGFPIIGESLEFFSTGWKGE 60

Query: 117 PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPS 176
           PEKFIFDR+NKYKS +FKTSIVG PAA+FCGP CNKFLFSNENKLVT+WWPDSVNKIFPS
Sbjct: 61  PEKFIFDRINKYKSHIFKTSIVGAPAAVFCGPTCNKFLFSNENKLVTAWWPDSVNKIFPS 120

Query: 177 STQTNSKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVFPLAKSL 236
           STQT+S EEAKKLKK LPQFLKPEAL RYIGIMD LA RHFAS WEN+++VLVFPLAKS 
Sbjct: 121 STQTSSMEEAKKLKKLLPQFLKPEALHRYIGIMDALAHRHFASSWENKDQVLVFPLAKS- 180

Query: 237 IEIQFLQSLILVDRLPIMELVSCSRSISISASSQSRSISFSVLIFLKLISDEIRFTFSLA 296
                                                                 FTFSLA
Sbjct: 181 ------------------------------------------------------FTFSLA 240

Query: 297 CRLFVSVDDEIQVERLSRPFEHIVAGIISLPIDLPGTPFNRAIKASNFIRKELVAIVRQR 356
           CRLFVSV+DE  VERLS PF+HI AGIIS+PID PGTPFNR IKASNFIRKEL++IV+QR
Sbjct: 241 CRLFVSVEDEAHVERLSGPFQHIAAGIISMPIDFPGTPFNRGIKASNFIRKELLSIVKQR 300

Query: 357 KWDLAEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVACTFILKF 416
           K DLAEGKASPTQDILSHMLLTCDENG YMNE DIADKILGLLIGGHDT S ACTFI+KF
Sbjct: 301 KVDLAEGKASPTQDILSHMLLTCDENGAYMNELDIADKILGLLIGGHDTASAACTFIVKF 360

Query: 417 LAELPHVYDGVYAEQMEIAKAKAPGEALKWEDINKMKYSWNVACEVLRIASPVQGAFREA 476
           LAELPH+YDGVY EQM IAKAKAPGE L WEDI KMKYSWNVACEVLR+ASP+QGAFREA
Sbjct: 361 LAELPHIYDGVYKEQMAIAKAKAPGELLNWEDIQKMKYSWNVACEVLRLASPLQGAFREA 388

Query: 477 LNDFVFNGFYIPKGWKVIFSPPS 500
           +NDFVFNGFYIPKGWK+ +S  S
Sbjct: 421 INDFVFNGFYIPKGWKLYWSSNS 388

BLAST of CaUC03G061480 vs. ExPASy TrEMBL
Match: A0A6J1JJQ7 (beta-amyrin 28-oxidase OS=Cucurbita maxima OX=3661 GN=LOC111485071 PE=3 SV=1)

HSP 1 Score: 641.0 bits (1652), Expect = 4.3e-180
Identity = 326/445 (73.26%), Postives = 353/445 (79.33%), Query Frame = 0

Query: 57  MEHFLISLLILFLFLLSLTFFILFHKHKSLFSYPNVPPGAIGFPILGESLEFLSTGWKGH 116
           ME FLISLLILFL  +SLT F+LF+KHKSLFSYPN PPGAIG PI+GESLEFLSTGWKG 
Sbjct: 1   MEDFLISLLILFLSFISLTLFVLFYKHKSLFSYPNTPPGAIGLPIIGESLEFLSTGWKGE 60

Query: 117 PEKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPS 176
           PEKFIFDRLNKYKSD+FKTSIVGVPAAIFCGP CNKFLFSNENKLVTSWWPDSVNKIFP+
Sbjct: 61  PEKFIFDRLNKYKSDIFKTSIVGVPAAIFCGPVCNKFLFSNENKLVTSWWPDSVNKIFPA 120

Query: 177 STQTNSKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVFPLAKSL 236
           ST+ NSKE A +LKK LP+FLKPEALQRYIG+MDELAQRHFAS WENRE VLVFPLAKS 
Sbjct: 121 STEINSKESAIRLKKLLPRFLKPEALQRYIGVMDELAQRHFASSWENREHVLVFPLAKS- 180

Query: 237 IEIQFLQSLILVDRLPIMELVSCSRSISISASSQSRSISFSVLIFLKLISDEIRFTFSLA 296
                                                                 FTFSLA
Sbjct: 181 ------------------------------------------------------FTFSLA 240

Query: 297 CRLFVSVDDEIQVERLSRPFEHIVAGIISLPIDLPGTPFNRAIKASNFIRKELVAIVRQR 356
           CRLF+S++D I VERLS PF++I AGIIS+PIDLPGTPFNRAIKASNF+RKEL++IVRQR
Sbjct: 241 CRLFLSLEDRIHVERLSEPFQNIAAGIISMPIDLPGTPFNRAIKASNFVRKELLSIVRQR 300

Query: 357 KWDLAEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVACTFILKF 416
           K DLAEGKASPTQDILSHMLLTCDE G YMNES IAD ILGLLIGGHDT S ACTFI+K+
Sbjct: 301 KSDLAEGKASPTQDILSHMLLTCDEIGQYMNESAIADNILGLLIGGHDTASAACTFIVKY 360

Query: 417 LAELPHVYDGVYAEQMEI--AKAKAPGEALKWEDINKMKYSWNVACEVLRIASPVQGAFR 476
           LAELP +YD VY EQMEI  AKAKA GE LKWEDI KMKYSWNVACEVLR+ASP+QGAFR
Sbjct: 361 LAELPQIYDRVYREQMEIAKAKAKAKGELLKWEDIQKMKYSWNVACEVLRLASPLQGAFR 390

Query: 477 EALNDFVFNGFYIPKGWKVIFSPPS 500
           EA+NDFVFNGF IPKGWK+ +S  S
Sbjct: 421 EAINDFVFNGFNIPKGWKLYWSSNS 390

BLAST of CaUC03G061480 vs. TAIR 10
Match: AT5G36110.1 (cytochrome P450, family 716, subfamily A, polypeptide 1 )

HSP 1 Score: 448.7 bits (1153), Expect = 6.0e-126
Identity = 227/440 (51.59%), Postives = 286/440 (65.00%), Query Frame = 0

Query: 60  FLISLLILFLFLLSLTFFILFHKHKSLFSYPNVPPGAIGFPILGESLEFLSTGWKGHPEK 119
           ++  ++ILFL  + L+  +L  KH S FSYPN+PPG  G P++GES  FLS G +GHPEK
Sbjct: 2   YMAIMIILFLSSILLSLLLLLRKHLSHFSYPNLPPGNTGLPLIGESFSFLSAGRQGHPEK 61

Query: 120 FIFDRLNKYKSD---VFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPS 179
           FI DR+ ++ S    VFKT + G P A+  G + NKFLF+NENKLV SWWPDSVNKIFPS
Sbjct: 62  FITDRVRRFSSSSSCVFKTHLFGSPTAVVTGASGNKFLFTNENKLVVSWWPDSVNKIFPS 121

Query: 180 STQTNSKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVFPLAKSL 239
           S QT+SKEEA+KL+  L QF+KPEAL+RY+G+MDE+AQRHF + W N+++V+VFPL K  
Sbjct: 122 SMQTSSKEEARKLRMLLSQFMKPEALRRYVGVMDEIAQRHFETEWANQDQVIVFPLTK-- 181

Query: 240 IEIQFLQSLILVDRLPIMELVSCSRSISISASSQSRSISFSVLIFLKLISDEIRFTFSLA 299
                                                                +FTFS+A
Sbjct: 182 -----------------------------------------------------KFTFSIA 241

Query: 300 CRLFVSVDDEIQVERLSRPFEHIVAGIISLPIDLPGTPFNRAIKASNFIRKELVAIVRQR 359
           CR F+S++D  +V +L   F  +  GI S+PIDLPGT FNRAIKAS  +RKE+ AIVRQR
Sbjct: 242 CRSFLSMEDPARVRQLEEQFNTVAVGIFSIPIDLPGTRFNRAIKASRLLRKEVSAIVRQR 301

Query: 360 KWDLAEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVACTFILKF 419
           K +L  GKA    DILSHML+   E      + D+ADKI+GLLIGGHDT S+ CTF++ +
Sbjct: 302 KEELKAGKALEEHDILSHMLMNIGET----KDEDLADKIIGLLIGGHDTASIVCTFVVNY 361

Query: 420 LAELPHVYDGVYAEQMEIAKAKAPGEALKWEDINKMKYSWNVACEVLRIASPVQGAFREA 479
           LAE PHVY  V  EQ EI K K   E L+WEDI KM+YSWNVACEV+RI  P+ G FREA
Sbjct: 362 LAEFPHVYQRVLQEQKEILKEKKEKEGLRWEDIEKMRYSWNVACEVMRIVPPLSGTFREA 382

Query: 480 LNDFVFNGFYIPKGWKVIFS 497
           ++ F F GFYIPKGWK+ +S
Sbjct: 422 IDHFSFKGFYIPKGWKLYWS 382

BLAST of CaUC03G061480 vs. TAIR 10
Match: AT5G36140.1 (cytochrome P450, family 716, subfamily A, polypeptide 2 )

HSP 1 Score: 353.6 bits (906), Expect = 2.6e-97
Identity = 184/373 (49.33%), Postives = 239/373 (64.08%), Query Frame = 0

Query: 64  LLILFLFLLSLTFFILFH--KHKSLFSYPNVPPGAIGFPILGESLEFLSTGWKGHPEKFI 123
           L I+FLF+ S+ F +LF   KH S F YPN+PPG IGFP++GE+L FLS G +GHPEKF+
Sbjct: 3   LTIIFLFISSIIFPLLFFLGKHLSNFRYPNLPPGKIGFPLIGETLSFLSAGRQGHPEKFV 62

Query: 124 FDRLNKYKSDVFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIFPSSTQTN 183
            DR+  + S +FKT + G P A+  G + NKFLF+NENKLV SWWPDSVNKIFPSSTQT+
Sbjct: 63  TDRVRHFSSGIFKTHLFGSPFAVVTGASGNKFLFTNENKLVISWWPDSVNKIFPSSTQTS 122

Query: 184 SKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVFPLAKSLIEIQF 243
           SKEEA K +  L   +KPEAL+RY+G+MDE+AQ+HF + W N+++++VFPL K       
Sbjct: 123 SKEEAIKTRMLLMPSMKPEALRRYVGVMDEIAQKHFETEWANQDQLIVFPLTK------- 182

Query: 244 LQSLILVDRLPIMELVSCSRSISISASSQSRSISFSVLIFLKLISDEIRFTFSLACRLFV 303
                                                           +FTFS+ACRLF+
Sbjct: 183 ------------------------------------------------KFTFSIACRLFL 242

Query: 304 SVDDEIQVERLSRPFEHIVAGIISLPIDLPGTPFNRAIKASNFIRKELVAIVRQRKWDLA 363
           S+DD  +V +L  PF  ++ G+ S+PIDLPGT FNRAIKAS  + KE+  I+RQRK +L 
Sbjct: 243 SMDDLERVRKLEEPFTTVMTGVFSIPIDLPGTRFNRAIKASRLLSKEVSTIIRQRKEELK 302

Query: 364 EGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVACTFILKFLAELP 423
            GK S  QDILSHML+   E      + D+ADKI+ LLIGGHDTTS+ CTF++ +LAE P
Sbjct: 303 AGKVSVEQDILSHMLMNIGET----KDEDLADKIIALLIGGHDTTSIVCTFVVNYLAEFP 315

Query: 424 HVYDGVYAEQMEI 435
           H+Y  V  E M+I
Sbjct: 363 HIYQRV-LEGMQI 315

BLAST of CaUC03G061480 vs. TAIR 10
Match: AT2G42850.1 (cytochrome P450, family 718 )

HSP 1 Score: 157.9 bits (398), Expect = 2.1e-38
Identity = 118/447 (26.40%), Postives = 200/447 (44.74%), Query Frame = 0

Query: 59  HFLISLLILFL-FLLSLTFFILFHKH-----KSLFSYPNVPPGAIGFPILGESLEFLSTG 118
           +F++S + L +   +S T F    KH     K +     + PG +G P +GE+++F    
Sbjct: 6   NFVLSWVFLCIAATISSTLFFFRKKHHRFITKKIQKKKKLLPGEMGLPWIGETMDFYKAQ 65

Query: 119 WKGHP-EKFIFDRLNKYKSDVFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWP-DSV 178
                 E F+  R+ K+  ++FKT I+G P  +  G   N+ + SNE  LV S WP  SV
Sbjct: 66  KSNRVFEDFVNPRIIKH-GNIFKTRIMGSPTIVVNGAEANRLILSNEFSLVVSSWPSSSV 125

Query: 179 NKIFPSSTQTNSKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVF 238
             +  +       E+ + L+  +   L    L+  I  + +  + H  + W  +EE+ ++
Sbjct: 126 QLMGMNCIMAKQGEKHRVLRGIVANSLSYIGLESLIPKLCDTVKFHHETEWRGKEEISLY 185

Query: 239 PLAKSLIEIQFLQSLILVDRLPIMELVSCSRSISISASSQSRSISFSVLIFLKLISDEIR 298
             AK L                                                      
Sbjct: 186 RSAKVL------------------------------------------------------ 245

Query: 299 FTFSLACRLFVSVDDEIQVERLSRPFEHIVAGIISLPIDLPGTPFNRAIKASNFIRKELV 358
            TF++       +  EI +  +   FE ++ G+ +LP++ P + F RA KA   I   LV
Sbjct: 246 -TFTVVFECLYGIKVEIGMLEV---FERVLEGVFALPVEFPCSKFARAKKARLEIETFLV 305

Query: 359 AIVRQRKWDL-AEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVA 418
             VR+++ ++  EG   P   + S ++    +    + E ++ D ++ L+   HDTTS A
Sbjct: 306 GKVREKRREMEKEGAEKPNTTLFSRLVEELIKG--VITEEEVVDNMVLLVFAAHDTTSYA 365

Query: 419 CTFILKFLAELPHVYDGVYAEQMEIAKAKAPGEALKWEDINKMKYSWNVACEVLRIASPV 478
            +   K LA+ P   D +  E  +I   K  GE L  ED+ KMKYSW V  E +R++ P+
Sbjct: 366 MSMTFKMLAQHPTCRDTLLQEHAQIKANKGEGEYLTVEDVKKMKYSWQVVRETMRLSPPI 391

Query: 479 QGAFREALNDFVFNGFYIPKGWKVIFS 497
            G+FR+A+ D  + G+ IPKGWK++++
Sbjct: 426 FGSFRKAVADIDYGGYTIPKGWKILWT 391

BLAST of CaUC03G061480 vs. TAIR 10
Match: AT5G45340.1 (cytochrome P450, family 707, subfamily A, polypeptide 3 )

HSP 1 Score: 151.4 bits (381), Expect = 2.0e-36
Identity = 122/434 (28.11%), Postives = 198/434 (45.62%), Query Frame = 0

Query: 62  ISLLILFLFLLSLTFFILFHKHKSLFSYPNVPPGAIGFPILGESLEFLSTGWKGHPEKFI 121
           +S   LFL LL    FI   +  S    P +PPG +G+P +GE+ +  S      P  F 
Sbjct: 10  LSAAALFLCLLR---FIAGVRRSSSTKLP-LPPGTMGYPYVGETFQLYSQ----DPNVFF 69

Query: 122 FDRLNKYKSDVFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIF-PSSTQT 181
             +  +Y S VFKT ++G P  +   P   KF+   ++ L    +P S  ++    +   
Sbjct: 70  AAKQRRYGS-VFKTHVLGCPCVMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFF 129

Query: 182 NSKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVFPLAKSLIEIQ 241
           +  +   KL+K + +   P+A++  +  ++ +AQ    S W+  +               
Sbjct: 130 HQGDYHSKLRKLVLRAFMPDAIRNMVPHIESIAQESLNS-WDGTQ--------------- 189

Query: 242 FLQSLILVDRLPIMELVSCSRSISISASSQSRSISFSVLIFLKLISDEIRFTFSLACRLF 301
                                   ++   + ++ +F+V +   L  DE+ +         
Sbjct: 190 ------------------------LNTYQEMKTYTFNVALISILGKDEVYYR-------- 249

Query: 302 VSVDDEIQVERLSRPFEHIVAGIISLPIDLPGTPFNRAIKASNFIRKELVAIVRQRKWDL 361
                    E L R +  +  G  S+PI+LPGT F++A+KA    RKEL  I+       
Sbjct: 250 ---------EDLKRCYYILEKGYNSMPINLPGTLFHKAMKA----RKELAQILANILSKR 309

Query: 362 AEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVACTFILKFLAEL 421
            +  +S T D+L   +    E+   + +  IAD I+G++    DTT+   T+ILK+LA+ 
Sbjct: 310 RQNPSSHT-DLLGSFM----EDKAGLTDEQIADNIIGVIFAARDTTASVLTWILKYLADN 368

Query: 422 PHVYDGVYAEQMEIAKAKAPGEALKWEDINKMKYSWNVACEVLRIASPVQGAFREALNDF 481
           P V + V  EQM I K K  GE+L WED  KM  ++ V  E LR A+ +   FREA+ D 
Sbjct: 370 PTVLEAVTEEQMAIRKDKKEGESLTWEDTKKMPLTYRVIQETLRAATILSFTFREAVEDV 368

Query: 482 VFNGFYIPKGWKVI 495
            + G+ IPKGWKV+
Sbjct: 430 EYEGYLIPKGWKVL 368

BLAST of CaUC03G061480 vs. TAIR 10
Match: AT5G45340.2 (cytochrome P450, family 707, subfamily A, polypeptide 3 )

HSP 1 Score: 151.4 bits (381), Expect = 2.0e-36
Identity = 122/434 (28.11%), Postives = 198/434 (45.62%), Query Frame = 0

Query: 62  ISLLILFLFLLSLTFFILFHKHKSLFSYPNVPPGAIGFPILGESLEFLSTGWKGHPEKFI 121
           +S   LFL LL    FI   +  S    P +PPG +G+P +GE+ +  S      P  F 
Sbjct: 10  LSAAALFLCLLR---FIAGVRRSSSTKLP-LPPGTMGYPYVGETFQLYSQ----DPNVFF 69

Query: 122 FDRLNKYKSDVFKTSIVGVPAAIFCGPACNKFLFSNENKLVTSWWPDSVNKIF-PSSTQT 181
             +  +Y S VFKT ++G P  +   P   KF+   ++ L    +P S  ++    +   
Sbjct: 70  AAKQRRYGS-VFKTHVLGCPCVMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFF 129

Query: 182 NSKEEAKKLKKFLPQFLKPEALQRYIGIMDELAQRHFASFWENREEVLVFPLAKSLIEIQ 241
           +  +   KL+K + +   P+A++  +  ++ +AQ    S W+  +               
Sbjct: 130 HQGDYHSKLRKLVLRAFMPDAIRNMVPHIESIAQESLNS-WDGTQ--------------- 189

Query: 242 FLQSLILVDRLPIMELVSCSRSISISASSQSRSISFSVLIFLKLISDEIRFTFSLACRLF 301
                                   ++   + ++ +F+V +   L  DE+ +         
Sbjct: 190 ------------------------LNTYQEMKTYTFNVALISILGKDEVYYR-------- 249

Query: 302 VSVDDEIQVERLSRPFEHIVAGIISLPIDLPGTPFNRAIKASNFIRKELVAIVRQRKWDL 361
                    E L R +  +  G  S+PI+LPGT F++A+KA    RKEL  I+       
Sbjct: 250 ---------EDLKRCYYILEKGYNSMPINLPGTLFHKAMKA----RKELAQILANILSKR 309

Query: 362 AEGKASPTQDILSHMLLTCDENGFYMNESDIADKILGLLIGGHDTTSVACTFILKFLAEL 421
            +  +S T D+L   +    E+   + +  IAD I+G++    DTT+   T+ILK+LA+ 
Sbjct: 310 RQNPSSHT-DLLGSFM----EDKAGLTDEQIADNIIGVIFAARDTTASVLTWILKYLADN 368

Query: 422 PHVYDGVYAEQMEIAKAKAPGEALKWEDINKMKYSWNVACEVLRIASPVQGAFREALNDF 481
           P V + V  EQM I K K  GE+L WED  KM  ++ V  E LR A+ +   FREA+ D 
Sbjct: 370 PTVLEAVTEEQMAIRKDKKEGESLTWEDTKKMPLTYRVIQETLRAATILSFTFREAVEDV 368

Query: 482 VFNGFYIPKGWKVI 495
            + G+ IPKGWKV+
Sbjct: 430 EYEGYLIPKGWKVL 368

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038877759.12.8e-19478.56LOW QUALITY PROTEIN: beta-amyrin 28-monooxygenase-like [Benincasa hispida][more]
XP_008443638.12.5e-19076.98PREDICTED: beta-amyrin 28-oxidase [Cucumis melo][more]
XP_004139087.14.0e-18876.30beta-amyrin 28-monooxygenase [Cucumis sativus] >KGN66615.1 hypothetical protein ... [more]
KAG6590124.11.7e-18374.49Beta-amyrin 28-monooxygenase, partial [Cucurbita argyrosperma subsp. sororia] >K... [more]
XP_022960871.12.3e-18374.49beta-amyrin 28-oxidase [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
F6H9N63.8e-15761.17Beta-amyrin 28-monooxygenase OS=Vitis vinifera OX=29760 GN=CYP716A15 PE=1 SV=1[more]
A5BFI42.1e-15560.72Beta-amyrin 28-monooxygenase OS=Vitis vinifera OX=29760 GN=CYP716A17 PE=1 SV=2[more]
A0A0S2II384.8e-15260.81Beta-amyrin 28-monooxygenase OS=Kalopanax septemlobus OX=228393 GN=CYP716A94 PE=... [more]
A0A3Q7HBJ51.1e-15160.50Beta-amyrin 28-monooxygenase OS=Solanum lycopersicum OX=4081 GN=CYP716A44 PE=1 S... [more]
K4CEE81.1e-15161.94Beta-amyrin 28-monooxygenase OS=Solanum lycopersicum OX=4081 GN=CYP716A46 PE=1 S... [more]
Match NameE-valueIdentityDescription
A0A1S3B8H41.2e-19076.98beta-amyrin 28-oxidase OS=Cucumis melo OX=3656 GN=LOC103487187 PE=3 SV=1[more]
A0A0A0LXN31.9e-18876.30Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G649550 PE=3 SV=1[more]
A0A6J1H8T41.1e-18374.49beta-amyrin 28-oxidase OS=Cucurbita moschata OX=3662 GN=LOC111461545 PE=3 SV=1[more]
A0A6J1DZX71.2e-18272.69beta-amyrin 28-oxidase OS=Momordica charantia OX=3673 GN=LOC111024635 PE=3 SV=1[more]
A0A6J1JJQ74.3e-18073.26beta-amyrin 28-oxidase OS=Cucurbita maxima OX=3661 GN=LOC111485071 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G36110.16.0e-12651.59cytochrome P450, family 716, subfamily A, polypeptide 1 [more]
AT5G36140.12.6e-9749.33cytochrome P450, family 716, subfamily A, polypeptide 2 [more]
AT2G42850.12.1e-3826.40cytochrome P450, family 718 [more]
AT5G45340.12.0e-3628.11cytochrome P450, family 707, subfamily A, polypeptide 3 [more]
AT5G45340.22.0e-3628.11cytochrome P450, family 707, subfamily A, polypeptide 3 [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (USVL246-FR2) v1
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 83..252
e-value: 3.3E-24
score: 87.4
coord: 268..501
e-value: 1.6E-47
score: 164.3
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 93..497
IPR001128Cytochrome P450PFAMPF00067p450coord: 331..498
e-value: 5.5E-28
score: 97.9
NoneNo IPR availablePANTHERPTHR24286CYTOCHROME P450 26coord: 63..235
NoneNo IPR availablePANTHERPTHR24286:SF297BETA-AMYRIN 28-OXIDASE-LIKEcoord: 63..235
coord: 289..499
NoneNo IPR availablePANTHERPTHR24286CYTOCHROME P450 26coord: 289..499

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CaUC03G061480.1CaUC03G061480.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016125 sterol metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen