CaUC03G058350 (gene) Watermelon (USVL246-FR2) v1

Overview
NameCaUC03G058350
Typegene
OrganismCitrullus amarus (Watermelon (USVL246-FR2) v1)
DescriptionFAD-binding Berberine family protein
LocationCiama_Chr03: 10057305 .. 10060238 (-)
RNA-Seq ExpressionCaUC03G058350
SyntenyCaUC03G058350
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATTTGGTTGAGGAGAATTTATCTTTTTCTTCTCTTTTGTGTTACTTTGATTTGGTTATGTTCTTGTAAATCTCTTCAAGAGAACTTCTTGGAATGCTTCAATTCCACATCTTATTCAAAGCATTCAATTCCCATTTCGGAAGTGGTTTACACAAACGAAAGTGCTTCTTTCTCCTCTCTGCTGCAGCTTTCCATTAGAAATCTCAGATTTCTTACCACTACTTCGCCTAAACCACTGTTTTTGGTGACCCCATTTCATGAGTCTCATGTCCAAGCAGCCATAGTTTGTGCTAGAAAGAAGGGTTTGCAAGTTCGAGTACGTAGTGGAGGACATGACTACGAAGGTTAGAACCTAAAAGCTTAAGTTTAATGTTACCGCTGTGTTTTAGTACATCAATGGTCGGAAAAATTACATTTTTGGTTATTAGGTTTTATGTTTAGTTTCCATTTTGTCTATAGGTTTTAAAATGTTACACTTTTAGTTCTTAAATTTTGAATTTGATTTCACTTAGTCCTTAGATTTCAAAATGTTATAATTTTACCTTTGAAATTTCAGCTTTGTATCAATTTGGCCTCTAGGTTTCATGATTTACACTTTTAAACTCGATTTTTTACTTAATATTCACTTCCCAACTTTAGTATGAAGAATCAAAATAATTATAATTAATTAAGTTTCATTATCTTACATCACTATACAAATTATAAATTTAAAATTTCATTTCACAACTATTTTAAATTAATTAATAGAGACTAAAAGTATTTTGTGAAAACTAGAGGTGTTAAAAGTTTAAATTTAATCTTTATACCTGGGAACCATAAATTGAAACAAAACTTAAATCTCAAGAATAAACTTGTAACATTGTGAAATTTAGGGACTAAATTGAAATAACTAAACTCAAAATTTAAAGGACTAGGAGTATATAACATTTTGAAACCTAGGGACCAAATGGAAACTAAACCCAAAACCTAAGGAGTAAAAAATATATATTTTTCCTTGATTGTTTTATATGCCTAGGAATAAGTACTGGTTCCTCTAGCCCTCGCCATGTCTGATATTTTTTGTAATCTCTCGATGCTTATTGAATTTTAATGAACGAGAGATGATGATGGTGTTAAGAAAGTGTCCACCTAGTGGAGATGTTTCGGTATACCTTATTTATTATTATTATTTTAAAAAAAAGTACTAATTGAAAATTTTGTATTAGTTGGCTAAATTTTAAAAGGTGGCAAAAAGTGATTTTTAACATATTAAATTCACTTTTAAAAAAATGTTATGGTGAAAAGTGAGTTGAAAAATTTTAAAAATAAATTAGAAGACACTTAAAATGCTTTTGGAAGAAGTTTTTTTGTTTCTTTTTTCAATATATATATATATATATATATATATATATATATATATCATCTTCACCAAAAGTGCTCATATATTTAATCACACATTTCTCCAAAGGCGCAGCGCACGGGGTAGTTTATAAGCATATCCATAAATTTAAATTCTTAACACTTTGAAAATTACCGCAAAGTTTAACACGCGAGTACCAATATTAATTAAACGGACAAACTCTATTCGTAGTATTACATGAAATCATCACTGAACCTAAAAGTTAACGATGTATTTGAACCAGGTCTCTCTTATGTATCATCACAAGCTCCGTTCATAGTAATTGATCTCATCAACCTTAGCTCCATAATTATCGACATCCAAACCGAAACAGCATTGGTGGGGACCGGTGCCACACTCGGCGAGTTGTACTACAGAATTGCAAAGAAAAGCCCCACCCATGGCTTTCCAGCAGGAAGCTGCCCCACAGTGGGAGTGGGAGGGCATATAAGTGGAGGAGGCTTTGGAACCCTTTTCAGAAAATATGGTCTTGCAGCTGATAATGTAATTGATGCTAAAATTGTTGACTTTAATGGAAGAATTATGGACAGAAACTCCATGGGAGAAGACCTTTTTTGGGCCATTAGAGGAGGTGGAGGAGCAAGCTTTGGAGTGATTTTATCATGGAAACTCAAACTAGTTTCTGTTCCTTCAATTGTCACTGTTTTCAATGTGCAAAGAACTCTAGAACAAGGAGCTACTCAACTTTTCCAAAAATGGCAAACCATTGGACACAAACTTCATCAAGATATTTTCCTTCATGTCACTACAAAAGTTACCAATTTTCCTTCCAAAAAAACAATTAGACTTTCATTTACATCATTGTTTCTTGGACCAATTGAAAGGCTTATCCCATTAATGAAGACTCATTTTCCAGAGTTGGGATTGAAGAGCATTGACTGCACTGAGATGAGTTGGATTCAATCAGTTCTCTTCTTTGCTGACCTGCCAACAGAAACCCCACTTGAAGTTTTGATGGAAAGATCACTTCCAGTTTCTGAATCATTTTTCACTGCAAAATCTGATTATGTTACATCACCAATTTCGCAAAATGGGTTGGAAGGGCTTTGGACTAAGCTGCTAGAGGAAGAAAAATCTGAGCTGATTTTGACACCATATGGGGGAAAAATGAGCCAAATTTCTGAATCACAAATCCCTTTTCCACATAGAAAAGGGAGCATTTTTGGGATTCAATATTTGGCAACTTGGGATAATGCTAATGAAACTGAGAAGCATTTGAGTTGGATAAGGGAGGTTTACACTTACATGGAACCCTATGTTTCCAAGTCTCCAAGAGCTGCTTATTTGAATTATAGGGATCTTGATTTGGGAAGAAACTATGGTAGAAATACAAGCTATGAACAGGCAAAAGTGTGGGGTTTGAAGTATTTCAGTGATAATTTTGAGAGATTGGTGAAGGTGAAGACCAAAGTTGATCCTTCAAACTTCTTTTGGAATGAACAGAGCATTCCTCTTCTCTATCATTATGAAGATGACACAAGAATTGCAAAGGTTTACTCAGGCTTGGACTTTGAAATTGTCCAAGAAAGATGA

mRNA sequence

ATGATTTGGTTGAGGAGAATTTATCTTTTTCTTCTCTTTTGTGTTACTTTGATTTGGTTATGTTCTTGTAAATCTCTTCAAGAGAACTTCTTGGAATGCTTCAATTCCACATCTTATTCAAAGCATTCAATTCCCATTTCGGAAGTGGTTTACACAAACGAAAGTGCTTCTTTCTCCTCTCTGCTGCAGCTTTCCATTAGAAATCTCAGATTTCTTACCACTACTTCGCCTAAACCACTGTTTTTGGTGACCCCATTTCATGAGTCTCATGTCCAAGCAGCCATAGTTTGTGCTAGAAAGAAGGGTTTGCAAGTTCGAGTACGTAGTGGAGGACATGACTACGAAGGTCTCTCTTATGTATCATCACAAGCTCCGTTCATAGTAATTGATCTCATCAACCTTAGCTCCATAATTATCGACATCCAAACCGAAACAGCATTGGTGGGGACCGGTGCCACACTCGGCGAGTTGTACTACAGAATTGCAAAGAAAAGCCCCACCCATGGCTTTCCAGCAGGAAGCTGCCCCACAGTGGGAGTGGGAGGGCATATAAGTGGAGGAGGCTTTGGAACCCTTTTCAGAAAATATGGTCTTGCAGCTGATAATGTAATTGATGCTAAAATTGTTGACTTTAATGGAAGAATTATGGACAGAAACTCCATGGGAGAAGACCTTTTTTGGGCCATTAGAGGAGGTGGAGGAGCAAGCTTTGGAGTGATTTTATCATGGAAACTCAAACTAGTTTCTGTTCCTTCAATTGTCACTGTTTTCAATGTGCAAAGAACTCTAGAACAAGGAGCTACTCAACTTTTCCAAAAATGGCAAACCATTGGACACAAACTTCATCAAGATATTTTCCTTCATGTCACTACAAAAGTTACCAATTTTCCTTCCAAAAAAACAATTAGACTTTCATTTACATCATTGTTTCTTGGACCAATTGAAAGGCTTATCCCATTAATGAAGACTCATTTTCCAGAGTTGGGATTGAAGAGCATTGACTGCACTGAGATGAGTTGGATTCAATCAGTTCTCTTCTTTGCTGACCTGCCAACAGAAACCCCACTTGAAGTTTTGATGGAAAGATCACTTCCAGTTTCTGAATCATTTTTCACTGCAAAATCTGATTATGTTACATCACCAATTTCGCAAAATGGGTTGGAAGGGCTTTGGACTAAGCTGCTAGAGGAAGAAAAATCTGAGCTGATTTTGACACCATATGGGGGAAAAATGAGCCAAATTTCTGAATCACAAATCCCTTTTCCACATAGAAAAGGGAGCATTTTTGGGATTCAATATTTGGCAACTTGGGATAATGCTAATGAAACTGAGAAGCATTTGAGTTGGATAAGGGAGGTTTACACTTACATGGAACCCTATGTTTCCAAGTCTCCAAGAGCTGCTTATTTGAATTATAGGGATCTTGATTTGGGAAGAAACTATGGTAGAAATACAAGCTATGAACAGGCAAAAGTGTGGGGTTTGAAGTATTTCAGTGATAATTTTGAGAGATTGGTGAAGGTGAAGACCAAAGTTGATCCTTCAAACTTCTTTTGGAATGAACAGAGCATTCCTCTTCTCTATCATTATGAAGATGACACAAGAATTGCAAAGGTTTACTCAGGCTTGGACTTTGAAATTGTCCAAGAAAGATGA

Coding sequence (CDS)

ATGATTTGGTTGAGGAGAATTTATCTTTTTCTTCTCTTTTGTGTTACTTTGATTTGGTTATGTTCTTGTAAATCTCTTCAAGAGAACTTCTTGGAATGCTTCAATTCCACATCTTATTCAAAGCATTCAATTCCCATTTCGGAAGTGGTTTACACAAACGAAAGTGCTTCTTTCTCCTCTCTGCTGCAGCTTTCCATTAGAAATCTCAGATTTCTTACCACTACTTCGCCTAAACCACTGTTTTTGGTGACCCCATTTCATGAGTCTCATGTCCAAGCAGCCATAGTTTGTGCTAGAAAGAAGGGTTTGCAAGTTCGAGTACGTAGTGGAGGACATGACTACGAAGGTCTCTCTTATGTATCATCACAAGCTCCGTTCATAGTAATTGATCTCATCAACCTTAGCTCCATAATTATCGACATCCAAACCGAAACAGCATTGGTGGGGACCGGTGCCACACTCGGCGAGTTGTACTACAGAATTGCAAAGAAAAGCCCCACCCATGGCTTTCCAGCAGGAAGCTGCCCCACAGTGGGAGTGGGAGGGCATATAAGTGGAGGAGGCTTTGGAACCCTTTTCAGAAAATATGGTCTTGCAGCTGATAATGTAATTGATGCTAAAATTGTTGACTTTAATGGAAGAATTATGGACAGAAACTCCATGGGAGAAGACCTTTTTTGGGCCATTAGAGGAGGTGGAGGAGCAAGCTTTGGAGTGATTTTATCATGGAAACTCAAACTAGTTTCTGTTCCTTCAATTGTCACTGTTTTCAATGTGCAAAGAACTCTAGAACAAGGAGCTACTCAACTTTTCCAAAAATGGCAAACCATTGGACACAAACTTCATCAAGATATTTTCCTTCATGTCACTACAAAAGTTACCAATTTTCCTTCCAAAAAAACAATTAGACTTTCATTTACATCATTGTTTCTTGGACCAATTGAAAGGCTTATCCCATTAATGAAGACTCATTTTCCAGAGTTGGGATTGAAGAGCATTGACTGCACTGAGATGAGTTGGATTCAATCAGTTCTCTTCTTTGCTGACCTGCCAACAGAAACCCCACTTGAAGTTTTGATGGAAAGATCACTTCCAGTTTCTGAATCATTTTTCACTGCAAAATCTGATTATGTTACATCACCAATTTCGCAAAATGGGTTGGAAGGGCTTTGGACTAAGCTGCTAGAGGAAGAAAAATCTGAGCTGATTTTGACACCATATGGGGGAAAAATGAGCCAAATTTCTGAATCACAAATCCCTTTTCCACATAGAAAAGGGAGCATTTTTGGGATTCAATATTTGGCAACTTGGGATAATGCTAATGAAACTGAGAAGCATTTGAGTTGGATAAGGGAGGTTTACACTTACATGGAACCCTATGTTTCCAAGTCTCCAAGAGCTGCTTATTTGAATTATAGGGATCTTGATTTGGGAAGAAACTATGGTAGAAATACAAGCTATGAACAGGCAAAAGTGTGGGGTTTGAAGTATTTCAGTGATAATTTTGAGAGATTGGTGAAGGTGAAGACCAAAGTTGATCCTTCAAACTTCTTTTGGAATGAACAGAGCATTCCTCTTCTCTATCATTATGAAGATGACACAAGAATTGCAAAGGTTTACTCAGGCTTGGACTTTGAAATTGTCCAAGAAAGATGA

Protein sequence

MIWLRRIYLFLLFCVTLIWLCSCKSLQENFLECFNSTSYSKHSIPISEVVYTNESASFSSLLQLSIRNLRFLTTTSPKPLFLVTPFHESHVQAAIVCARKKGLQVRVRSGGHDYEGLSYVSSQAPFIVIDLINLSSIIIDIQTETALVGTGATLGELYYRIAKKSPTHGFPAGSCPTVGVGGHISGGGFGTLFRKYGLAADNVIDAKIVDFNGRIMDRNSMGEDLFWAIRGGGGASFGVILSWKLKLVSVPSIVTVFNVQRTLEQGATQLFQKWQTIGHKLHQDIFLHVTTKVTNFPSKKTIRLSFTSLFLGPIERLIPLMKTHFPELGLKSIDCTEMSWIQSVLFFADLPTETPLEVLMERSLPVSESFFTAKSDYVTSPISQNGLEGLWTKLLEEEKSELILTPYGGKMSQISESQIPFPHRKGSIFGIQYLATWDNANETEKHLSWIREVYTYMEPYVSKSPRAAYLNYRDLDLGRNYGRNTSYEQAKVWGLKYFSDNFERLVKVKTKVDPSNFFWNEQSIPLLYHYEDDTRIAKVYSGLDFEIVQER
Homology
BLAST of CaUC03G058350 vs. NCBI nr
Match: XP_038895849.1 (berberine bridge enzyme-like 8 [Benincasa hispida])

HSP 1 Score: 1004.2 bits (2595), Expect = 4.3e-289
Identity = 506/554 (91.34%), Postives = 525/554 (94.77%), Query Frame = 0

Query: 1   MIWLRRIYLFLLFCVTLIWLCSCK-SLQENFLECFNSTSYSKHSIPISEVVYTNESASFS 60
           M+ LRR++LFLLF V+LIWLCS   SLQE FLECFNSTSYSKHSIPISEVV+TNESASFS
Sbjct: 1   MLCLRRVHLFLLFFVSLIWLCSSSDSLQERFLECFNSTSYSKHSIPISEVVFTNESASFS 60

Query: 61  SLLQLSIRNLRFLTTTSPKPLFLVTPFHESHVQAAIVCARKKGLQVRVRSGGHDYEGLSY 120
           SLLQLSIRNLRFLTTT PKPLFLVTPFHESHVQAAIVCARKKGLQVRVRSGGHDYEGLSY
Sbjct: 61  SLLQLSIRNLRFLTTTMPKPLFLVTPFHESHVQAAIVCARKKGLQVRVRSGGHDYEGLSY 120

Query: 121 VSSQAPFIVIDLINLSSIIIDIQTETALVGTGATLGELYYRIAKKSPTHGFPAGSCPTVG 180
           VSSQAPFIVIDLINL SI IDI+TET  V TGATLGELYY+IA+KSP HGFPAGSCPTVG
Sbjct: 121 VSSQAPFIVIDLINLHSITIDIETETTSVETGATLGELYYKIAEKSPIHGFPAGSCPTVG 180

Query: 181 VGGHISGGGFGTLFRKYGLAADNVIDAKIVDFNGRIMDRNSMGEDLFWAIRGGGGASFGV 240
           VGGH+SGGGFGTLFRKYGLAADNVIDAKIVDFNGRIMDRNSMGEDLFWAIRGGGGASFGV
Sbjct: 181 VGGHVSGGGFGTLFRKYGLAADNVIDAKIVDFNGRIMDRNSMGEDLFWAIRGGGGASFGV 240

Query: 241 ILSWKLKLVSVPSIVTVFNVQRTLEQGATQLFQKWQTIGHKLHQDIFLHVTTKV-TNFPS 300
           ILSWKLKLVSVPSIVTVFNVQRTLEQGAT LFQKWQTI HKL QDIFLHVTTKV TNF S
Sbjct: 241 ILSWKLKLVSVPSIVTVFNVQRTLEQGATHLFQKWQTIAHKLDQDIFLHVTTKVITNFSS 300

Query: 301 KKTIRLSFTSLFLGPIERLIPLMKTHFPELGLKSIDCTEMSWIQSVLFFADLPTETPLEV 360
           KKTIRLSFTSLFLGPIERLIP+MKTHF ELGLK  DCTEMSWIQSVLFFAD   ETPLEV
Sbjct: 301 KKTIRLSFTSLFLGPIERLIPIMKTHFSELGLKRNDCTEMSWIQSVLFFADFSIETPLEV 360

Query: 361 LMERSL-PVSESFFTAKSDYVTSPISQNGLEGLWTKLLEEEKSELILTPYGGKMSQISES 420
           LMERS  P+S++FFTAKSDYVTSPISQNGLEGLWTKLLEEEKSELILTPYGGKMSQISES
Sbjct: 361 LMERSSPPISDAFFTAKSDYVTSPISQNGLEGLWTKLLEEEKSELILTPYGGKMSQISES 420

Query: 421 QIPFPHRKGSIFGIQYLATWDNANETEKHLSWIREVYTYMEPYVSKSPRAAYLNYRDLDL 480
           Q PFPHR+GSIFGIQYLATWDNANETEKHLSWIREVYTYMEPYVSKSPRAAYLNYRDLDL
Sbjct: 421 QTPFPHRRGSIFGIQYLATWDNANETEKHLSWIREVYTYMEPYVSKSPRAAYLNYRDLDL 480

Query: 481 GRNYGRNTSYEQAKVWGLKYFSDNFERLVKVKTKVDPSNFFWNEQSIPLLYHYEDDTRIA 540
           GRNY +NTSYE+AKVWGLKYFSDNFERLV+VKTKVDP NFFWNEQSIPLLYHYEDDTRI+
Sbjct: 481 GRNYRKNTSYEEAKVWGLKYFSDNFERLVRVKTKVDPLNFFWNEQSIPLLYHYEDDTRIS 540

Query: 541 KVYSGLDFEIVQER 552
           KV+SGLDFEIVQER
Sbjct: 541 KVHSGLDFEIVQER 554

BLAST of CaUC03G058350 vs. NCBI nr
Match: XP_008445632.1 (PREDICTED: flavin-dependent oxidoreductase FOX5-like [Cucumis melo] >KAA0047127.1 flavin-dependent oxidoreductase FOX5-like [Cucumis melo var. makuwa] >TYK14944.1 flavin-dependent oxidoreductase FOX5-like [Cucumis melo var. makuwa])

HSP 1 Score: 988.0 bits (2553), Expect = 3.2e-284
Identity = 497/554 (89.71%), Postives = 518/554 (93.50%), Query Frame = 0

Query: 1   MIWLRRIYLFLLFCVTLIWLCSCK-SLQENFLECFNSTSYSKHSIPISEVVYTNESASFS 60
           MI LRR +LFLLFCV+LIWLCS   SLQ+NFLECFNSTSYSKHSIP+SEVV+TNESASFS
Sbjct: 3   MICLRRTHLFLLFCVSLIWLCSSSDSLQDNFLECFNSTSYSKHSIPVSEVVFTNESASFS 62

Query: 61  SLLQLSIRNLRFLTTTSPKPLFLVTPFHESHVQAAIVCARKKGLQVRVRSGGHDYEGLSY 120
           SL +LSIRNLRFLTTT PKPLFLVTPFHESHVQAAIVCAR+KGLQVRVRSGGHDYEGLSY
Sbjct: 63  SLFRLSIRNLRFLTTTLPKPLFLVTPFHESHVQAAIVCAREKGLQVRVRSGGHDYEGLSY 122

Query: 121 VSSQAPFIVIDLINLSSIIIDIQTETALVGTGATLGELYYRIAKKSPTHGFPAGSCPTVG 180
           VSSQAPFIVIDLINL SI IDI+ ETA VGTGA LGELYYRIAKKS  HGFPAGSCPTVG
Sbjct: 123 VSSQAPFIVIDLINLRSITIDIEDETASVGTGAALGELYYRIAKKSSIHGFPAGSCPTVG 182

Query: 181 VGGHISGGGFGTLFRKYGLAADNVIDAKIVDFNGRIMDRNSMGEDLFWAIRGGGGASFGV 240
           VGGHISGGGFGTLFRKYGLAADNVIDAKI+DFNGRIMDR+SMGEDLFWAIRGGGGASFGV
Sbjct: 183 VGGHISGGGFGTLFRKYGLAADNVIDAKIIDFNGRIMDRSSMGEDLFWAIRGGGGASFGV 242

Query: 241 ILSWKLKLVSVPSIVTVFNVQRTLEQGATQLFQKWQTIGHKLHQDIFLHVTTK-VTNFPS 300
           ILSWKLKLVSVPSIVTVFNVQRTLEQGAT LFQKWQ I HKL QDIFLHVTTK VT+FPS
Sbjct: 243 ILSWKLKLVSVPSIVTVFNVQRTLEQGATHLFQKWQNISHKLDQDIFLHVTTKVVTDFPS 302

Query: 301 KKTIRLSFTSLFLGPIERLIPLMKTHFPELGLKSIDCTEMSWIQSVLFFADLPTETPLEV 360
           KKTIRLSFTSLFLGPIERLIP+MKT F ELGLK  DC EMSWIQSVLFFAD   +TPLEV
Sbjct: 303 KKTIRLSFTSLFLGPIERLIPIMKTRFSELGLKRSDCIEMSWIQSVLFFADFSIDTPLEV 362

Query: 361 LMERSLP-VSESFFTAKSDYVTSPISQNGLEGLWTKLLEEEKSELILTPYGGKMSQISES 420
           LMERS P +S++FFTAKSDYV SPISQ GLEGLW KLLEEEKSELILTPYGGKMSQISES
Sbjct: 363 LMERSSPQISDAFFTAKSDYVISPISQKGLEGLWKKLLEEEKSELILTPYGGKMSQISES 422

Query: 421 QIPFPHRKGSIFGIQYLATWDNANETEKHLSWIREVYTYMEPYVSKSPRAAYLNYRDLDL 480
           Q PFPHR+G IFGIQYLATWDNANETEKHLSWIREVY YM+PYVSKSPRAAYLNYRDLDL
Sbjct: 423 QTPFPHREGRIFGIQYLATWDNANETEKHLSWIREVYAYMKPYVSKSPRAAYLNYRDLDL 482

Query: 481 GRNYGRNTSYEQAKVWGLKYFSDNFERLVKVKTKVDPSNFFWNEQSIPLLYHYEDDTRIA 540
           GRNYGRNTSYE+AKVWGLKYFSDNFERLV+VKTKVDPSNFFWNEQSIPLLYHYEDDTRI 
Sbjct: 483 GRNYGRNTSYEEAKVWGLKYFSDNFERLVRVKTKVDPSNFFWNEQSIPLLYHYEDDTRIT 542

Query: 541 KVYSGLDFEIVQER 552
           KV+SGLDFEIVQER
Sbjct: 543 KVHSGLDFEIVQER 556

BLAST of CaUC03G058350 vs. NCBI nr
Match: KAE8653305.1 (hypothetical protein Csa_023319 [Cucumis sativus])

HSP 1 Score: 973.4 bits (2515), Expect = 8.2e-280
Identity = 487/555 (87.75%), Postives = 517/555 (93.15%), Query Frame = 0

Query: 1   MIWLRRIYLFLLFCVTLIWLC--SCKSLQENFLECFNSTSYSKHSIPISEVVYTNESASF 60
           MI  RRI+LFLL CV+LIWLC  S  SLQ+NFLECFNSTSYSKHSIPISEVV+TNESASF
Sbjct: 3   MICFRRIHLFLLLCVSLIWLCGSSSDSLQDNFLECFNSTSYSKHSIPISEVVFTNESASF 62

Query: 61  SSLLQLSIRNLRFLTTTSPKPLFLVTPFHESHVQAAIVCARKKGLQVRVRSGGHDYEGLS 120
           SSLL+LSIRNLRFLTTT PKPL LVTPFHESHVQAAI+CAR+KGLQVRVRSGGHDYEGLS
Sbjct: 63  SSLLRLSIRNLRFLTTTLPKPLLLVTPFHESHVQAAILCAREKGLQVRVRSGGHDYEGLS 122

Query: 121 YVSSQAPFIVIDLINLSSIIIDIQTETALVGTGATLGELYYRIAKKSPTHGFPAGSCPTV 180
           Y+SSQAPFIVIDLINL SI IDI+TETA V TGA+LGELYYRIAKKS  HGFPAGSCPTV
Sbjct: 123 YISSQAPFIVIDLINLRSIKIDIKTETASVETGASLGELYYRIAKKSSIHGFPAGSCPTV 182

Query: 181 GVGGHISGGGFGTLFRKYGLAADNVIDAKIVDFNGRIMDRNSMGEDLFWAIRGGGGASFG 240
           GVGGHISGGGFGTLFRKYGLAADNVIDAKI+DFNGRIMDR+SMGEDLFWAIRGGGGASFG
Sbjct: 183 GVGGHISGGGFGTLFRKYGLAADNVIDAKIIDFNGRIMDRSSMGEDLFWAIRGGGGASFG 242

Query: 241 VILSWKLKLVSVPSIVTVFNVQRTLEQGATQLFQKWQTIGHKLHQDIFLHVTTK-VTNFP 300
           VILSWKLKLVSVPSIVTVFNVQRTLE+GAT LFQKWQ I HKL QDIFLHVTTK VTNFP
Sbjct: 243 VILSWKLKLVSVPSIVTVFNVQRTLEEGATHLFQKWQNISHKLDQDIFLHVTTKVVTNFP 302

Query: 301 SKKTIRLSFTSLFLGPIERLIPLMKTHFPELGLKSIDCTEMSWIQSVLFFADLPTETPLE 360
           SKKTIRLSFTSLFLGPIERLIP+MKT F ELGLK  DC EMSWIQSVLFFAD   + PLE
Sbjct: 303 SKKTIRLSFTSLFLGPIERLIPIMKTRFSELGLKRNDCIEMSWIQSVLFFADFSIDAPLE 362

Query: 361 VLMERSLP-VSESFFTAKSDYVTSPISQNGLEGLWTKLLEEEKSELILTPYGGKMSQISE 420
           VLM+RS P +S++FFTAKSDYVTSPIS+NGLEGLW+KLLEE+KSELI TPYGGKMSQISE
Sbjct: 363 VLMDRSSPQISDAFFTAKSDYVTSPISENGLEGLWSKLLEEDKSELIFTPYGGKMSQISE 422

Query: 421 SQIPFPHRKGSIFGIQYLATWDNANETEKHLSWIREVYTYMEPYVSKSPRAAYLNYRDLD 480
           SQIPFPHR+G IFGIQYLATWDNANE EKHLSWIREVY YME YVSKSPRAAYLNYRDLD
Sbjct: 423 SQIPFPHREGRIFGIQYLATWDNANENEKHLSWIREVYAYMESYVSKSPRAAYLNYRDLD 482

Query: 481 LGRNYGRNTSYEQAKVWGLKYFSDNFERLVKVKTKVDPSNFFWNEQSIPLLYHYEDDTRI 540
           LG NYGRNTSYE+AKVWGLKYFSDNF+RLV+VKTKVDPSNFFWNEQSIPLLYHYEDDT++
Sbjct: 483 LGTNYGRNTSYEEAKVWGLKYFSDNFKRLVRVKTKVDPSNFFWNEQSIPLLYHYEDDTKV 542

Query: 541 AKVYSGLDFEIVQER 552
            KV+SGLDFEI+QER
Sbjct: 543 TKVHSGLDFEIIQER 557

BLAST of CaUC03G058350 vs. NCBI nr
Match: XP_004135724.1 (berberine bridge enzyme-like 13 [Cucumis sativus])

HSP 1 Score: 973.4 bits (2515), Expect = 8.2e-280
Identity = 487/555 (87.75%), Postives = 517/555 (93.15%), Query Frame = 0

Query: 1   MIWLRRIYLFLLFCVTLIWLC--SCKSLQENFLECFNSTSYSKHSIPISEVVYTNESASF 60
           MI  RRI+LFLL CV+LIWLC  S  SLQ+NFLECFNSTSYSKHSIPISEVV+TNESASF
Sbjct: 1   MICFRRIHLFLLLCVSLIWLCGSSSDSLQDNFLECFNSTSYSKHSIPISEVVFTNESASF 60

Query: 61  SSLLQLSIRNLRFLTTTSPKPLFLVTPFHESHVQAAIVCARKKGLQVRVRSGGHDYEGLS 120
           SSLL+LSIRNLRFLTTT PKPL LVTPFHESHVQAAI+CAR+KGLQVRVRSGGHDYEGLS
Sbjct: 61  SSLLRLSIRNLRFLTTTLPKPLLLVTPFHESHVQAAILCAREKGLQVRVRSGGHDYEGLS 120

Query: 121 YVSSQAPFIVIDLINLSSIIIDIQTETALVGTGATLGELYYRIAKKSPTHGFPAGSCPTV 180
           Y+SSQAPFIVIDLINL SI IDI+TETA V TGA+LGELYYRIAKKS  HGFPAGSCPTV
Sbjct: 121 YISSQAPFIVIDLINLRSIKIDIKTETASVETGASLGELYYRIAKKSSIHGFPAGSCPTV 180

Query: 181 GVGGHISGGGFGTLFRKYGLAADNVIDAKIVDFNGRIMDRNSMGEDLFWAIRGGGGASFG 240
           GVGGHISGGGFGTLFRKYGLAADNVIDAKI+DFNGRIMDR+SMGEDLFWAIRGGGGASFG
Sbjct: 181 GVGGHISGGGFGTLFRKYGLAADNVIDAKIIDFNGRIMDRSSMGEDLFWAIRGGGGASFG 240

Query: 241 VILSWKLKLVSVPSIVTVFNVQRTLEQGATQLFQKWQTIGHKLHQDIFLHVTTK-VTNFP 300
           VILSWKLKLVSVPSIVTVFNVQRTLE+GAT LFQKWQ I HKL QDIFLHVTTK VTNFP
Sbjct: 241 VILSWKLKLVSVPSIVTVFNVQRTLEEGATHLFQKWQNISHKLDQDIFLHVTTKVVTNFP 300

Query: 301 SKKTIRLSFTSLFLGPIERLIPLMKTHFPELGLKSIDCTEMSWIQSVLFFADLPTETPLE 360
           SKKTIRLSFTSLFLGPIERLIP+MKT F ELGLK  DC EMSWIQSVLFFAD   + PLE
Sbjct: 301 SKKTIRLSFTSLFLGPIERLIPIMKTRFSELGLKRNDCIEMSWIQSVLFFADFSIDAPLE 360

Query: 361 VLMERSLP-VSESFFTAKSDYVTSPISQNGLEGLWTKLLEEEKSELILTPYGGKMSQISE 420
           VLM+RS P +S++FFTAKSDYVTSPIS+NGLEGLW+KLLEE+KSELI TPYGGKMSQISE
Sbjct: 361 VLMDRSSPQISDAFFTAKSDYVTSPISENGLEGLWSKLLEEDKSELIFTPYGGKMSQISE 420

Query: 421 SQIPFPHRKGSIFGIQYLATWDNANETEKHLSWIREVYTYMEPYVSKSPRAAYLNYRDLD 480
           SQIPFPHR+G IFGIQYLATWDNANE EKHLSWIREVY YME YVSKSPRAAYLNYRDLD
Sbjct: 421 SQIPFPHREGRIFGIQYLATWDNANENEKHLSWIREVYAYMESYVSKSPRAAYLNYRDLD 480

Query: 481 LGRNYGRNTSYEQAKVWGLKYFSDNFERLVKVKTKVDPSNFFWNEQSIPLLYHYEDDTRI 540
           LG NYGRNTSYE+AKVWGLKYFSDNF+RLV+VKTKVDPSNFFWNEQSIPLLYHYEDDT++
Sbjct: 481 LGTNYGRNTSYEEAKVWGLKYFSDNFKRLVRVKTKVDPSNFFWNEQSIPLLYHYEDDTKV 540

Query: 541 AKVYSGLDFEIVQER 552
            KV+SGLDFEI+QER
Sbjct: 541 TKVHSGLDFEIIQER 555

BLAST of CaUC03G058350 vs. NCBI nr
Match: XP_022140178.1 (berberine bridge enzyme-like 8 [Momordica charantia])

HSP 1 Score: 892.1 bits (2304), Expect = 2.4e-255
Identity = 448/558 (80.29%), Postives = 494/558 (88.53%), Query Frame = 0

Query: 6   RIYLFLLF--CVTLIWLCSCKSLQENFLECFNSTSYSKHSIPISEVVYTNESASFSSLLQ 65
           RI+LFL+F  CV+     S   LQE+F+ECFNST+YSKHS+PISEVV+TNESASFSSLL+
Sbjct: 8   RIHLFLIFFHCVS-----SSDCLQESFMECFNSTTYSKHSVPISEVVFTNESASFSSLLR 67

Query: 66  LSIRNLRFLTTTSPKPLFLVTPFHESHVQAAIVCARKKGLQVRVRSGGHDYEGLSYVSSQ 125
           LSIRNLRFLT +SPKP FLVTPF  SHVQAA+VCA+KK LQVRVRSGGHDYEGLSYVSSQ
Sbjct: 68  LSIRNLRFLTNSSPKPQFLVTPFRASHVQAAVVCAKKKSLQVRVRSGGHDYEGLSYVSSQ 127

Query: 126 APFIVIDLINLSSIIIDIQTETALVGTGATLGELYYRIAKKSPTHGFPAGSCPTVGVGGH 185
            PFIVIDLINLSSI IDI+TETA V TGATLGE+YYRIA+KSP HGFPAGSCPTVGVGGH
Sbjct: 128 TPFIVIDLINLSSIHIDIETETASVETGATLGEVYYRIAEKSPIHGFPAGSCPTVGVGGH 187

Query: 186 ISGGGFGTLFRKYGLAADNVIDAKIVDFNGRIMDRNSMGEDLFWAIRGGGGASFGVILSW 245
           +SGGGFGTLFRKYGLAADNVIDAKIVDFNGRI DRNSMGEDLFWAIRGGGGASFGVILSW
Sbjct: 188 VSGGGFGTLFRKYGLAADNVIDAKIVDFNGRIRDRNSMGEDLFWAIRGGGGASFGVILSW 247

Query: 246 KLKLVSVPSIVTVFNVQRTLEQGATQLFQKWQTIGHKLHQDIFLHVTTKVTNFPSK---- 305
           KLKLVSVP  VTVFNV RTL+QGAT LFQKWQ I HK+ QD+FLHVTTKV +  +K    
Sbjct: 248 KLKLVSVPPDVTVFNVHRTLDQGATHLFQKWQKIAHKMDQDLFLHVTTKVVDHKTKLSSS 307

Query: 306 -----KTIRLSFTSLFLGPIERLIPLMKTHFPELGLKSIDCTEMSWIQSVLFFADLPTET 365
                KTI+LSFTSLFLGPIERLIPLM THF ELGLK  DCTEMSWIQSVLFFADL TET
Sbjct: 308 KEASSKTIKLSFTSLFLGPIERLIPLMNTHFHELGLKRNDCTEMSWIQSVLFFADLSTET 367

Query: 366 PLEVLMERSLPVSESFFTAKSDYVTSPISQNGLEGLWTKLLEEEKSELILTPYGGKMSQI 425
           P E+LMERS P+S++FFTAKSDYVTSPISQ GLE LW+KLLEEEKSELILTPYGGKM QI
Sbjct: 368 PPEILMERSPPISDTFFTAKSDYVTSPISQTGLEALWSKLLEEEKSELILTPYGGKMDQI 427

Query: 426 SESQIPFPHRKGSIFGIQYLATWDNANETEKHLSWIREVYTYMEPYVSKSPRAAYLNYRD 485
           SES+ PFPHR+G+I+GIQYLA W+NANETEKHLSW+REVY+YM+P+VSK PR AYLNYRD
Sbjct: 428 SESETPFPHRRGNIYGIQYLAAWENANETEKHLSWMREVYSYMQPFVSKYPRGAYLNYRD 487

Query: 486 LDLGRNYGRNTSYEQAKVWGLKYFSDNFERLVKVKTKVDPSNFFWNEQSIPLLYHYEDD- 545
           LDLGRNY RNTSY++AKVWGLKYF  NFERLV+VKTKVDP NFFWNEQSIPL +H+E++ 
Sbjct: 488 LDLGRNYERNTSYDEAKVWGLKYFKGNFERLVRVKTKVDPLNFFWNEQSIPLCHHHEENG 547

Query: 546 TRIAKVYSGLDFEIVQER 552
           TRI +V S LD +I+QER
Sbjct: 548 TRILQVNSYLDSKILQER 560

BLAST of CaUC03G058350 vs. ExPASy Swiss-Prot
Match: O64743 (Berberine bridge enzyme-like 15 OS=Arabidopsis thaliana OX=3702 GN=MEE23 PE=1 SV=1)

HSP 1 Score: 537.7 bits (1384), Expect = 1.5e-151
Identity = 267/522 (51.15%), Postives = 361/522 (69.16%), Query Frame = 0

Query: 12  LFCVTLIWL---CSCKSLQENFLECFNSTSYSKHSIPISEVVYTNESASFSSLLQLSIRN 71
           LF VTL+ +    S  +LQ++F++C    S     I  S       +  F   L+ + +N
Sbjct: 12  LFLVTLLLISVPLSSSTLQQDFVKCLVDNSDVSFPITASFFSPDQNATLFKEELESTAQN 71

Query: 72  LRFLTTTSPKPLFLVTPFHESHVQAAIVCARKKGLQVRVRSGGHDYEGLSYVS-SQAPFI 131
           LR+LT ++PKP+F+  P +E+HVQAA+VCA+K  L +R+RSGGHDYEGLS+V+  + PF+
Sbjct: 72  LRYLTPSNPKPVFIFEPLYETHVQAAVVCAKKLQLHLRLRSGGHDYEGLSFVAEDETPFV 131

Query: 132 VIDLINLSSIIIDIQTETALVGTGATLGELYYRIAKKSPTHGFPAGSCPTVGVGGHISGG 191
           ++DL  L  + +D+ + +A    GAT+GE+YYRI +KS THGFPAG C ++G+GGH+ GG
Sbjct: 132 IVDLSKLRQVDVDLDSNSAWAHAGATIGEVYYRIQEKSQTHGFPAGLCSSLGIGGHLVGG 191

Query: 192 GFGTLFRKYGLAADNVIDAKIVDFNGRIMDRNSMGEDLFWAIRGGGGASFGVILSWKLKL 251
            +G++ RK+GL ADNV+DA+IVD NG+I+DR +MGED+FWAIRGGGG SFGVIL+WK+KL
Sbjct: 192 AYGSMMRKFGLGADNVLDARIVDANGQILDRAAMGEDVFWAIRGGGGGSFGVILAWKIKL 251

Query: 252 VSVPSIVTVFNVQRTLEQGATQLFQKWQTIGHKLHQDIFLHV---TTKVTNFPSKKTIRL 311
           V VP+ VTVF V +TLEQ  T++  KW+ I  KL  D+F+ V       T  P  +TI +
Sbjct: 252 VPVPATVTVFTVTKTLEQDGTKVLYKWEQIADKLDDDLFIRVIISPASKTTKPGNRTISM 311

Query: 312 SFTSLFLGPIERLIPLMKTHFPELGLKSIDCTEMSWIQSVLFFADLPTETPLEVLMERSL 371
           S+ + FLG   RL+ +M+  FPELGL   DCTEMSWI+SV++ A  P     E L+    
Sbjct: 312 SYQAQFLGDSNRLLQVMQKSFPELGLTKKDCTEMSWIKSVMYIAGFPNSAAPEALL-AGK 371

Query: 372 PVSESFFTAKSDYVTSPISQNGLEGLWTKLLEEEKSELILTPYGGKMSQISESQIPFPHR 431
            + ++ F AKSD+V  PI   GLEGLW + LEE+    I  PYGG MS+ISES+IPFPHR
Sbjct: 372 SLFKNHFKAKSDFVKEPIPVEGLEGLWERFLEEDSPLTIWNPYGGMMSRISESEIPFPHR 431

Query: 432 KGSIFGIQYLATW-DNANETEKHLSWIREVYTYMEPYVSKSPRAAYLNYRDLDLGRNYGR 491
            G++F IQ+L+TW D     E+H+ WIRE+Y+YME YVSK+PR AY+NYRDLDLG N G 
Sbjct: 432 NGTLFKIQWLSTWQDGKVSEERHMKWIREMYSYMEQYVSKNPRQAYVNYRDLDLGTNEGE 491

Query: 492 NTSYEQAKVWGLKYFSDNFERLVKVKTKVDPSNFFWNEQSIP 526
                 A+ WG KY+  NFERLVK+K + DP NFF +EQS+P
Sbjct: 492 T----DAREWGAKYYKGNFERLVKIKGEFDPDNFFRHEQSVP 528

BLAST of CaUC03G058350 vs. ExPASy Swiss-Prot
Match: Q9FKU8 (Berberine bridge enzyme-like 26 OS=Arabidopsis thaliana OX=3702 GN=At5g44400 PE=2 SV=1)

HSP 1 Score: 535.8 bits (1379), Expect = 5.7e-151
Identity = 264/523 (50.48%), Postives = 364/523 (69.60%), Query Frame = 0

Query: 7   IYLFLLFCVTLIWLCSCKSLQENFLECFNSTSYSKHSIPISEVVYTNESASFSSLLQLSI 66
           +  F L+ +T     S  SLQ+ F+ C    ++    +  +    T   + FS +L+ + 
Sbjct: 14  VLYFSLYTITP--TSSLASLQDQFINCVQRNTHVYFPLEKTFFAPTKNVSMFSQVLESTA 73

Query: 67  RNLRFLTTTSPKPLFLVTPFHESHVQAAIVCARKKGLQVRVRSGGHDYEGLSYVSS-QAP 126
           +NLRFL  + PKP F+ +P HESHVQA+I+C++K  + +RVRSGGHDYEGLSYVS    P
Sbjct: 74  QNLRFLKKSMPKPGFIFSPIHESHVQASIICSKKLRMHLRVRSGGHDYEGLSYVSQIDKP 133

Query: 127 FIVIDLINLSSIIIDIQTETALVGTGATLGELYYRIAKKSPTHGFPAGSCPTVGVGGHIS 186
           FI++DL  +  + I+IQ  +A V +GAT+GELYYRIA+KS  HGFPAG C ++G+GGHI+
Sbjct: 134 FILMDLSKMRQVNINIQDNSAWVQSGATVGELYYRIAEKSKVHGFPAGLCSSLGIGGHIT 193

Query: 187 GGGFGTLFRKYGLAADNVIDAKIVDFNGRIMDRNSMGEDLFWAIRGGGGASFGVILSWKL 246
           GG +G++ RKYGL ADNV+DAKIVD NG+++DR +MGED FWAIRGG G SFG+IL+WK+
Sbjct: 194 GGAYGSMMRKYGLGADNVLDAKIVDANGKLLDRAAMGEDTFWAIRGGAGGSFGIILAWKI 253

Query: 247 KLVSVPSIVTVFNVQRTLEQG-ATQLFQKWQTIGHKLHQDIFLHVTTKVTNFPSKKTIRL 306
           KLV VP  VTVF V +TL+Q    ++  KWQ +  KL +++F+ V   V      KT+  
Sbjct: 254 KLVPVPKTVTVFTVTKTLQQDVGNKIISKWQRVADKLVEELFIRVLFNVAGTGGNKTVTT 313

Query: 307 SFTSLFLGPIERLIPLMKTHFPELGLKSIDCTEMSWIQSVLFFADLPTETPLEVLMERSL 366
           S+ +LFLG    L+ +MK  FPELGL   DC EMSW++S+ + +  PT TP  VL++   
Sbjct: 314 SYNALFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLESIAYISGFPTHTPTNVLLQGKS 373

Query: 367 PVSESFFTAKSDYVTSPISQNGLEGLWTKLLEEEKSELILTPYGGKMSQISESQIPFPHR 426
           P  +  F AKSD+V +PI ++GL+G++ KLL+E+   +I  PYGG M++I ESQIPFPHR
Sbjct: 374 PFPKVSFKAKSDFVKTPIPESGLQGIFKKLLKEDIPLMIWNPYGGMMAKIPESQIPFPHR 433

Query: 427 KGSIFGIQYLATW-DNANETEKHLSWIREVYTYMEPYVSKSPRAAYLNYRDLDLGRN-YG 486
           KG +F +QY+ +W D+     +H++WIR++Y+YM PYVS +PR AY+NYRDLDLGRN   
Sbjct: 434 KGVLFKVQYVTSWLDSDKRPSRHINWIRDLYSYMTPYVSSNPREAYVNYRDLDLGRNTKD 493

Query: 487 RNTSYEQAKVWGLKYFSDNFERLVKVKTKVDPSNFFWNEQSIP 526
             T  +QA+VWG  YF +NF RL+ +K KVDP NFF +EQSIP
Sbjct: 494 VKTCIKQAQVWGANYFKNNFNRLMMIKAKVDPENFFRHEQSIP 534

BLAST of CaUC03G058350 vs. ExPASy Swiss-Prot
Match: Q9SA85 (Berberine bridge enzyme-like 8 OS=Arabidopsis thaliana OX=3702 GN=At1g30700 PE=2 SV=1)

HSP 1 Score: 535.0 bits (1377), Expect = 9.8e-151
Identity = 265/525 (50.48%), Postives = 366/525 (69.71%), Query Frame = 0

Query: 9   LFLLFCVTLIWLCSCKSL-QENFLECFNSTSYSKHSIPISEVVYTNESASFSSLLQLSIR 68
           + +LF V  IW  S  S   E F +C  S S  KH  PIS  ++ + + S+SS+LQ +IR
Sbjct: 6   ILVLFFVVFIWQSSSSSANSETFTQCLTSNSDPKH--PISPAIFFSGNGSYSSVLQANIR 65

Query: 69  NLRFLTTTSPKPLFLVTPFHESHVQAAIVCARKKGLQVRVRSGGHDYEGLSYVS-SQAPF 128
           NLRF TT++PKP  ++   HESHVQAAI C ++  LQ+++RSGGHDY+GLSYV+ S  PF
Sbjct: 66  NLRFNTTSTPKPFLIIAATHESHVQAAITCGKRHNLQMKIRSGGHDYDGLSYVTYSGKPF 125

Query: 129 IVIDLINLSSIIIDIQTETALVGTGATLGELYYRIAKKSPTHGFPAGSCPTVGVGGHISG 188
            V+D+ NL S+ +D+ ++TA V TGA LGE+YY I +KS T  +PAG CPTVGVGGHISG
Sbjct: 126 FVLDMFNLRSVDVDVASKTAWVQTGAILGEVYYYIWEKSKTLAYPAGICPTVGVGGHISG 185

Query: 189 GGFGTLFRKYGLAADNVIDAKIVDFNGRIMDRNSMGEDLFWAIRGGGGASFGVILSWKLK 248
           GG+G + RKYGL  DN IDA++VD NG+I+DR  MGEDL+WAI GGGG S+GV+L++K+ 
Sbjct: 186 GGYGNMMRKYGLTVDNTIDARMVDVNGKILDRKLMGEDLYWAINGGGGGSYGVVLAYKIN 245

Query: 249 LVSVPSIVTVFNVQRTLEQGATQLFQKWQTIGHKLHQDIFLHVTTKVTN--FPSKKTIRL 308
           LV VP  VTVF + RTLEQ AT +  +WQ +  KL  ++F+     V N    S+KT+R 
Sbjct: 246 LVEVPENVTVFRISRTLEQNATDIIHRWQQVAPKLPDELFIRTVIDVVNGTVSSQKTVRT 305

Query: 309 SFTSLFLGPIERLIPLMKTHFPELGLKSIDCTEMSWIQSVLFFADLPTETPLEVLMERSL 368
           +F ++FLG    L+ ++   FPELGL   DCTE SWIQSVLF+ ++   +   +L++R+ 
Sbjct: 306 TFIAMFLGDTTTLLSILNRRFPELGLVRSDCTETSWIQSVLFWTNIQVGSSETLLLQRNQ 365

Query: 369 PVSESFFTAKSDYVTSPISQNGLEGLWTKLLEEEKSELILTPYGGKMSQISESQIPFPHR 428
           PV  ++   KSDYV  PIS+ GLE +W K++E E   +   PYGG+M +IS +  PFP+R
Sbjct: 366 PV--NYLKRKSDYVREPISRTGLESIWKKMIELEIPTMAFNPYGGEMGRISSTVTPFPYR 425

Query: 429 KGSIFGIQYLATWDNANETEKHLSWIREVYTYMEPYVSKSPRAAYLNYRDLDLGRN--YG 488
            G+++ IQY A W +   T++++   R++Y +M P+VSK+PR ++ NYRD+DLG N   G
Sbjct: 426 AGNLWKIQYGANWRDETLTDRYMELTRKLYQFMTPFVSKNPRQSFFNYRDVDLGINSHNG 485

Query: 489 RNTSYEQAKVWGLKYFSDNFERLVKVKTKVDPSNFFWNEQSIPLL 528
           + +SY + K +G KYF+ NFERLVK+KT+VD  NFF NEQSIP+L
Sbjct: 486 KISSYVEGKRYGKKYFAGNFERLVKIKTRVDSGNFFRNEQSIPVL 526

BLAST of CaUC03G058350 vs. ExPASy Swiss-Prot
Match: Q9FKU9 (Berberine bridge enzyme-like 25 OS=Arabidopsis thaliana OX=3702 GN=At5g44390 PE=2 SV=1)

HSP 1 Score: 534.3 bits (1375), Expect = 1.7e-150
Identity = 267/523 (51.05%), Postives = 362/523 (69.22%), Query Frame = 0

Query: 11  LLFCVTLIWLCSCKSLQENFLECFNSTSYSKHSIPISEVVYTNESASFSSLLQLSIRNLR 70
           L F    I L S  SLQ++F++C    +  + ++  +       ++ F+ +L+ + +N R
Sbjct: 18  LYFSFYTITLTSSTSLQDDFIKCLYRNTNVRFTLDKTFFTPERNASIFTEVLESTAQNQR 77

Query: 71  FLTTTSPKPLFLVTPFHESHVQAAIVCARKKGLQVRVRSGGHDYEGLSYVSS-QAPFIVI 130
           +LT T PKP F+  P HESHVQA+++C++K  +  RVRSGGHDYEG+SYVS  + PF++I
Sbjct: 78  YLTKTMPKPGFIFKPVHESHVQASVICSKKLEIHFRVRSGGHDYEGVSYVSQIEKPFVLI 137

Query: 131 DLINLSSIIIDIQTETALVGTGATLGELYYRIAKKSPTHGFPAGSCPTVGVGGHISGGGF 190
           DL  L  I +DI+  +A V  GAT+GELYYRIA+KS  HGFPAG  P++G+GGHI+GG +
Sbjct: 138 DLSKLRQINVDIKDTSAWVEAGATVGELYYRIAEKSKFHGFPAGVYPSLGIGGHITGGAY 197

Query: 191 GTLFRKYGLAADNVIDAKIVDFNGRIMDRNSMGEDLFWAIRGGGGASFGVILSWKLKLVS 250
           G+L RKYGLAADNV+DAKIVD NG+++DR SMGEDLFWAIRGG G SFG+ILSWK+KLV 
Sbjct: 198 GSLMRKYGLAADNVLDAKIVDANGKLLDRASMGEDLFWAIRGGSGGSFGIILSWKIKLVP 257

Query: 251 VPSIVTVFNVQRTLEQGAT-QLFQKWQTIGHKLHQDIFLHVTTKVTNFPSKKTIRLSFTS 310
           VP  +TVF V +T EQ  + ++  KWQ I   L  ++FL V   V+   + KT+ +++  
Sbjct: 258 VPETLTVFTVTKTFEQDRSFKILSKWQEIADNLVDELFLRVFFTVSGNKANKTVTMAYIG 317

Query: 311 LFLGPIERLIPLMKTHFPELGLKSIDCTEMSWIQSVLFFADLPTE--TPLEVLMERSLPV 370
            FLG    L+ +MK  FPELGL   DC EMSWI S+++ +  PT    P+E+L++   P+
Sbjct: 318 QFLGEKGTLMEVMKKDFPELGLTQKDCIEMSWIDSIIYNSGFPTNPPPPIEILLQAKSPI 377

Query: 371 SESFFTAKSDYVTSPISQNGLEGLWTKLLEEEKSELILTPYGGKMSQISESQIPFPHRKG 430
            + +F  KSD+   PI   GLEG++ KLLEE+ + +I TPYGGKM +I ES+IPFPHR G
Sbjct: 378 GKVYFKGKSDFAKKPIPVLGLEGMFKKLLEEDAALVIWTPYGGKMDKIPESEIPFPHRNG 437

Query: 431 SIFGIQYLATW-DNANETEKHLSWIREVYTYMEPYVSKSPRAAYLNYRDLDLGRNYGRNT 490
           + F IQY  +W D+     +   WIRE+Y YM PYVS +PR AY+NYRDLDLG+N   + 
Sbjct: 438 TNFMIQYYRSWSDSEKRPNRRTKWIRELYGYMTPYVSSNPRQAYVNYRDLDLGQNKDNSK 497

Query: 491 S-YEQAKVWGLKYFSDNFERLVKVKTKVDPSNFFWNEQSIPLL 528
           S + +AK+WG  YF DNF RLV++K+KVDP NFF +EQSIP L
Sbjct: 498 SNFIEAKIWGANYFKDNFNRLVRIKSKVDPDNFFRHEQSIPTL 540

BLAST of CaUC03G058350 vs. ExPASy Swiss-Prot
Match: Q93ZA3 (Berberine bridge enzyme-like 13 OS=Arabidopsis thaliana OX=3702 GN=At1g30760 PE=1 SV=1)

HSP 1 Score: 530.4 bits (1365), Expect = 2.4e-149
Identity = 266/524 (50.76%), Postives = 365/524 (69.66%), Query Frame = 0

Query: 13  FCVTLIWL------CSCKSLQENFLECFNSTSYSKHSIPISEVVYTNESASFSSLLQLSI 72
           F VTL+ L       S  ++Q++F+ C    S +   +  S   +   ++SF   L+ S 
Sbjct: 12  FLVTLLLLSLSYIPLSFSTIQQDFVMCLVDNSDASFPMDSSFFTHDLNASSFKLALETSA 71

Query: 73  RNLRFLTTTSPKPLFLVTPFHESHVQAAIVCARKKGLQVRVRSGGHDYEGLSYVSS-QAP 132
           +NLR+L  ++PKP F+  P +E+HVQAA++CA+K  L +R+RSGGHDYEGLSYVS  +  
Sbjct: 72  QNLRYLMPSNPKPEFIFEPLYETHVQAAVLCAKKLKLHLRLRSGGHDYEGLSYVSEMETA 131

Query: 133 FIVIDLINLSSIIIDIQTETALVGTGATLGELYYRIAKKSPTHGFPAGSCPTVGVGGHIS 192
           F+++DL  L  I +DI++ +A V  GA++GE+YYRI +KS  HGFPAG C ++G+GGHI 
Sbjct: 132 FVIVDLSKLRQISVDIESNSAWVHAGASIGEVYYRIQEKSKIHGFPAGLCTSLGIGGHII 191

Query: 193 GGGFGTLFRKYGLAADNVIDAKIVDFNGRIMDRNSMGEDLFWAIRGGGGASFGVILSWKL 252
           GG +G++ RK+GL ADNV+DA+IVD +G+I++R +MGED+FWAIRGGGG SFGVIL+WK+
Sbjct: 192 GGAYGSMMRKFGLGADNVLDARIVDADGKILNRAAMGEDVFWAIRGGGGGSFGVILAWKI 251

Query: 253 KLVSVPSIVTVFNVQRTLEQGATQLFQKWQTIGHKLHQDIFLHVTTKVTNFPSK---KTI 312
           KLV VP IVTVF V RTLEQ  T+L  KWQ +  KL +D+F+ V  + T+   K   +TI
Sbjct: 252 KLVPVPEIVTVFTVTRTLEQDGTKLLYKWQQVADKLDEDLFIRVIIQPTSKTPKSKERTI 311

Query: 313 RLSFTSLFLGPIERLIPLMKTHFPELGLKSIDCTEMSWIQSVLFFADLPTETPLEVLMER 372
             S+   FLG   RL+ +M+  FP+LGL   DC E SWI+SV++ A  P+  P E L++ 
Sbjct: 312 STSYQGQFLGDANRLLQVMQRSFPQLGLTKKDCLETSWIKSVMYIAGFPSTAPSEALLD- 371

Query: 373 SLPVSESFFTAKSDYVTSPISQNGLEGLWTKLLEEEKSELILTPYGGKMSQISESQIPFP 432
              + +++F AKSDYV  PI   GLEGLW KLLEE+    I  PYGG M++I E++ PFP
Sbjct: 372 GKSLFKNYFKAKSDYVEEPIPVEGLEGLWEKLLEEDSPLTIWNPYGGMMAKIPETETPFP 431

Query: 433 HRKGSIFGIQYLATWDNANETE-KHLSWIREVYTYMEPYVSKSPRAAYLNYRDLDLGRNY 492
           HR G++F IQ+L  W +   +E KH+ W+RE+Y+YME YVSKSPR+AY+NYRDLDLG N 
Sbjct: 432 HRSGTLFKIQWLTLWQDGKTSEAKHMGWMREMYSYMEQYVSKSPRSAYVNYRDLDLGMN- 491

Query: 493 GRNTSYEQAKVWGLKYFSDNFERLVKVKTKVDPSNFFWNEQSIP 526
           G+ +    A+ WG +YF  NFERLV++K K DP NFF +EQSIP
Sbjct: 492 GKGS---DAREWGNRYFKGNFERLVEIKAKFDPENFFRHEQSIP 530

BLAST of CaUC03G058350 vs. ExPASy TrEMBL
Match: A0A5A7TYX1 (Flavin-dependent oxidoreductase FOX5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold437G00060 PE=3 SV=1)

HSP 1 Score: 988.0 bits (2553), Expect = 1.6e-284
Identity = 497/554 (89.71%), Postives = 518/554 (93.50%), Query Frame = 0

Query: 1   MIWLRRIYLFLLFCVTLIWLCSCK-SLQENFLECFNSTSYSKHSIPISEVVYTNESASFS 60
           MI LRR +LFLLFCV+LIWLCS   SLQ+NFLECFNSTSYSKHSIP+SEVV+TNESASFS
Sbjct: 3   MICLRRTHLFLLFCVSLIWLCSSSDSLQDNFLECFNSTSYSKHSIPVSEVVFTNESASFS 62

Query: 61  SLLQLSIRNLRFLTTTSPKPLFLVTPFHESHVQAAIVCARKKGLQVRVRSGGHDYEGLSY 120
           SL +LSIRNLRFLTTT PKPLFLVTPFHESHVQAAIVCAR+KGLQVRVRSGGHDYEGLSY
Sbjct: 63  SLFRLSIRNLRFLTTTLPKPLFLVTPFHESHVQAAIVCAREKGLQVRVRSGGHDYEGLSY 122

Query: 121 VSSQAPFIVIDLINLSSIIIDIQTETALVGTGATLGELYYRIAKKSPTHGFPAGSCPTVG 180
           VSSQAPFIVIDLINL SI IDI+ ETA VGTGA LGELYYRIAKKS  HGFPAGSCPTVG
Sbjct: 123 VSSQAPFIVIDLINLRSITIDIEDETASVGTGAALGELYYRIAKKSSIHGFPAGSCPTVG 182

Query: 181 VGGHISGGGFGTLFRKYGLAADNVIDAKIVDFNGRIMDRNSMGEDLFWAIRGGGGASFGV 240
           VGGHISGGGFGTLFRKYGLAADNVIDAKI+DFNGRIMDR+SMGEDLFWAIRGGGGASFGV
Sbjct: 183 VGGHISGGGFGTLFRKYGLAADNVIDAKIIDFNGRIMDRSSMGEDLFWAIRGGGGASFGV 242

Query: 241 ILSWKLKLVSVPSIVTVFNVQRTLEQGATQLFQKWQTIGHKLHQDIFLHVTTK-VTNFPS 300
           ILSWKLKLVSVPSIVTVFNVQRTLEQGAT LFQKWQ I HKL QDIFLHVTTK VT+FPS
Sbjct: 243 ILSWKLKLVSVPSIVTVFNVQRTLEQGATHLFQKWQNISHKLDQDIFLHVTTKVVTDFPS 302

Query: 301 KKTIRLSFTSLFLGPIERLIPLMKTHFPELGLKSIDCTEMSWIQSVLFFADLPTETPLEV 360
           KKTIRLSFTSLFLGPIERLIP+MKT F ELGLK  DC EMSWIQSVLFFAD   +TPLEV
Sbjct: 303 KKTIRLSFTSLFLGPIERLIPIMKTRFSELGLKRSDCIEMSWIQSVLFFADFSIDTPLEV 362

Query: 361 LMERSLP-VSESFFTAKSDYVTSPISQNGLEGLWTKLLEEEKSELILTPYGGKMSQISES 420
           LMERS P +S++FFTAKSDYV SPISQ GLEGLW KLLEEEKSELILTPYGGKMSQISES
Sbjct: 363 LMERSSPQISDAFFTAKSDYVISPISQKGLEGLWKKLLEEEKSELILTPYGGKMSQISES 422

Query: 421 QIPFPHRKGSIFGIQYLATWDNANETEKHLSWIREVYTYMEPYVSKSPRAAYLNYRDLDL 480
           Q PFPHR+G IFGIQYLATWDNANETEKHLSWIREVY YM+PYVSKSPRAAYLNYRDLDL
Sbjct: 423 QTPFPHREGRIFGIQYLATWDNANETEKHLSWIREVYAYMKPYVSKSPRAAYLNYRDLDL 482

Query: 481 GRNYGRNTSYEQAKVWGLKYFSDNFERLVKVKTKVDPSNFFWNEQSIPLLYHYEDDTRIA 540
           GRNYGRNTSYE+AKVWGLKYFSDNFERLV+VKTKVDPSNFFWNEQSIPLLYHYEDDTRI 
Sbjct: 483 GRNYGRNTSYEEAKVWGLKYFSDNFERLVRVKTKVDPSNFFWNEQSIPLLYHYEDDTRIT 542

Query: 541 KVYSGLDFEIVQER 552
           KV+SGLDFEIVQER
Sbjct: 543 KVHSGLDFEIVQER 556

BLAST of CaUC03G058350 vs. ExPASy TrEMBL
Match: A0A1S3BE11 (flavin-dependent oxidoreductase FOX5-like OS=Cucumis melo OX=3656 GN=LOC103488588 PE=3 SV=1)

HSP 1 Score: 988.0 bits (2553), Expect = 1.6e-284
Identity = 497/554 (89.71%), Postives = 518/554 (93.50%), Query Frame = 0

Query: 1   MIWLRRIYLFLLFCVTLIWLCSCK-SLQENFLECFNSTSYSKHSIPISEVVYTNESASFS 60
           MI LRR +LFLLFCV+LIWLCS   SLQ+NFLECFNSTSYSKHSIP+SEVV+TNESASFS
Sbjct: 3   MICLRRTHLFLLFCVSLIWLCSSSDSLQDNFLECFNSTSYSKHSIPVSEVVFTNESASFS 62

Query: 61  SLLQLSIRNLRFLTTTSPKPLFLVTPFHESHVQAAIVCARKKGLQVRVRSGGHDYEGLSY 120
           SL +LSIRNLRFLTTT PKPLFLVTPFHESHVQAAIVCAR+KGLQVRVRSGGHDYEGLSY
Sbjct: 63  SLFRLSIRNLRFLTTTLPKPLFLVTPFHESHVQAAIVCAREKGLQVRVRSGGHDYEGLSY 122

Query: 121 VSSQAPFIVIDLINLSSIIIDIQTETALVGTGATLGELYYRIAKKSPTHGFPAGSCPTVG 180
           VSSQAPFIVIDLINL SI IDI+ ETA VGTGA LGELYYRIAKKS  HGFPAGSCPTVG
Sbjct: 123 VSSQAPFIVIDLINLRSITIDIEDETASVGTGAALGELYYRIAKKSSIHGFPAGSCPTVG 182

Query: 181 VGGHISGGGFGTLFRKYGLAADNVIDAKIVDFNGRIMDRNSMGEDLFWAIRGGGGASFGV 240
           VGGHISGGGFGTLFRKYGLAADNVIDAKI+DFNGRIMDR+SMGEDLFWAIRGGGGASFGV
Sbjct: 183 VGGHISGGGFGTLFRKYGLAADNVIDAKIIDFNGRIMDRSSMGEDLFWAIRGGGGASFGV 242

Query: 241 ILSWKLKLVSVPSIVTVFNVQRTLEQGATQLFQKWQTIGHKLHQDIFLHVTTK-VTNFPS 300
           ILSWKLKLVSVPSIVTVFNVQRTLEQGAT LFQKWQ I HKL QDIFLHVTTK VT+FPS
Sbjct: 243 ILSWKLKLVSVPSIVTVFNVQRTLEQGATHLFQKWQNISHKLDQDIFLHVTTKVVTDFPS 302

Query: 301 KKTIRLSFTSLFLGPIERLIPLMKTHFPELGLKSIDCTEMSWIQSVLFFADLPTETPLEV 360
           KKTIRLSFTSLFLGPIERLIP+MKT F ELGLK  DC EMSWIQSVLFFAD   +TPLEV
Sbjct: 303 KKTIRLSFTSLFLGPIERLIPIMKTRFSELGLKRSDCIEMSWIQSVLFFADFSIDTPLEV 362

Query: 361 LMERSLP-VSESFFTAKSDYVTSPISQNGLEGLWTKLLEEEKSELILTPYGGKMSQISES 420
           LMERS P +S++FFTAKSDYV SPISQ GLEGLW KLLEEEKSELILTPYGGKMSQISES
Sbjct: 363 LMERSSPQISDAFFTAKSDYVISPISQKGLEGLWKKLLEEEKSELILTPYGGKMSQISES 422

Query: 421 QIPFPHRKGSIFGIQYLATWDNANETEKHLSWIREVYTYMEPYVSKSPRAAYLNYRDLDL 480
           Q PFPHR+G IFGIQYLATWDNANETEKHLSWIREVY YM+PYVSKSPRAAYLNYRDLDL
Sbjct: 423 QTPFPHREGRIFGIQYLATWDNANETEKHLSWIREVYAYMKPYVSKSPRAAYLNYRDLDL 482

Query: 481 GRNYGRNTSYEQAKVWGLKYFSDNFERLVKVKTKVDPSNFFWNEQSIPLLYHYEDDTRIA 540
           GRNYGRNTSYE+AKVWGLKYFSDNFERLV+VKTKVDPSNFFWNEQSIPLLYHYEDDTRI 
Sbjct: 483 GRNYGRNTSYEEAKVWGLKYFSDNFERLVRVKTKVDPSNFFWNEQSIPLLYHYEDDTRIT 542

Query: 541 KVYSGLDFEIVQER 552
           KV+SGLDFEIVQER
Sbjct: 543 KVHSGLDFEIVQER 556

BLAST of CaUC03G058350 vs. ExPASy TrEMBL
Match: A0A6J1CHF0 (berberine bridge enzyme-like 8 OS=Momordica charantia OX=3673 GN=LOC111010911 PE=3 SV=1)

HSP 1 Score: 892.1 bits (2304), Expect = 1.2e-255
Identity = 448/558 (80.29%), Postives = 494/558 (88.53%), Query Frame = 0

Query: 6   RIYLFLLF--CVTLIWLCSCKSLQENFLECFNSTSYSKHSIPISEVVYTNESASFSSLLQ 65
           RI+LFL+F  CV+     S   LQE+F+ECFNST+YSKHS+PISEVV+TNESASFSSLL+
Sbjct: 8   RIHLFLIFFHCVS-----SSDCLQESFMECFNSTTYSKHSVPISEVVFTNESASFSSLLR 67

Query: 66  LSIRNLRFLTTTSPKPLFLVTPFHESHVQAAIVCARKKGLQVRVRSGGHDYEGLSYVSSQ 125
           LSIRNLRFLT +SPKP FLVTPF  SHVQAA+VCA+KK LQVRVRSGGHDYEGLSYVSSQ
Sbjct: 68  LSIRNLRFLTNSSPKPQFLVTPFRASHVQAAVVCAKKKSLQVRVRSGGHDYEGLSYVSSQ 127

Query: 126 APFIVIDLINLSSIIIDIQTETALVGTGATLGELYYRIAKKSPTHGFPAGSCPTVGVGGH 185
            PFIVIDLINLSSI IDI+TETA V TGATLGE+YYRIA+KSP HGFPAGSCPTVGVGGH
Sbjct: 128 TPFIVIDLINLSSIHIDIETETASVETGATLGEVYYRIAEKSPIHGFPAGSCPTVGVGGH 187

Query: 186 ISGGGFGTLFRKYGLAADNVIDAKIVDFNGRIMDRNSMGEDLFWAIRGGGGASFGVILSW 245
           +SGGGFGTLFRKYGLAADNVIDAKIVDFNGRI DRNSMGEDLFWAIRGGGGASFGVILSW
Sbjct: 188 VSGGGFGTLFRKYGLAADNVIDAKIVDFNGRIRDRNSMGEDLFWAIRGGGGASFGVILSW 247

Query: 246 KLKLVSVPSIVTVFNVQRTLEQGATQLFQKWQTIGHKLHQDIFLHVTTKVTNFPSK---- 305
           KLKLVSVP  VTVFNV RTL+QGAT LFQKWQ I HK+ QD+FLHVTTKV +  +K    
Sbjct: 248 KLKLVSVPPDVTVFNVHRTLDQGATHLFQKWQKIAHKMDQDLFLHVTTKVVDHKTKLSSS 307

Query: 306 -----KTIRLSFTSLFLGPIERLIPLMKTHFPELGLKSIDCTEMSWIQSVLFFADLPTET 365
                KTI+LSFTSLFLGPIERLIPLM THF ELGLK  DCTEMSWIQSVLFFADL TET
Sbjct: 308 KEASSKTIKLSFTSLFLGPIERLIPLMNTHFHELGLKRNDCTEMSWIQSVLFFADLSTET 367

Query: 366 PLEVLMERSLPVSESFFTAKSDYVTSPISQNGLEGLWTKLLEEEKSELILTPYGGKMSQI 425
           P E+LMERS P+S++FFTAKSDYVTSPISQ GLE LW+KLLEEEKSELILTPYGGKM QI
Sbjct: 368 PPEILMERSPPISDTFFTAKSDYVTSPISQTGLEALWSKLLEEEKSELILTPYGGKMDQI 427

Query: 426 SESQIPFPHRKGSIFGIQYLATWDNANETEKHLSWIREVYTYMEPYVSKSPRAAYLNYRD 485
           SES+ PFPHR+G+I+GIQYLA W+NANETEKHLSW+REVY+YM+P+VSK PR AYLNYRD
Sbjct: 428 SESETPFPHRRGNIYGIQYLAAWENANETEKHLSWMREVYSYMQPFVSKYPRGAYLNYRD 487

Query: 486 LDLGRNYGRNTSYEQAKVWGLKYFSDNFERLVKVKTKVDPSNFFWNEQSIPLLYHYEDD- 545
           LDLGRNY RNTSY++AKVWGLKYF  NFERLV+VKTKVDP NFFWNEQSIPL +H+E++ 
Sbjct: 488 LDLGRNYERNTSYDEAKVWGLKYFKGNFERLVRVKTKVDPLNFFWNEQSIPLCHHHEENG 547

Query: 546 TRIAKVYSGLDFEIVQER 552
           TRI +V S LD +I+QER
Sbjct: 548 TRILQVNSYLDSKILQER 560

BLAST of CaUC03G058350 vs. ExPASy TrEMBL
Match: A0A6J1JUS4 (berberine bridge enzyme-like 15 OS=Cucurbita maxima OX=3661 GN=LOC111489931 PE=3 SV=1)

HSP 1 Score: 882.9 bits (2280), Expect = 7.1e-253
Identity = 445/559 (79.61%), Postives = 488/559 (87.30%), Query Frame = 0

Query: 1   MIWLRRIYLFLLFCVTLIWLCSCKSL---QENFLECFNSTSYSKHSIPISEVVYTNESAS 60
           M+  RRI L L F V LIW+CS  S    QENFLECFNST+YSKHS+P+SEVV++NESAS
Sbjct: 1   MVHWRRIQLLLFFWVPLIWICSSSSSDFDQENFLECFNSTTYSKHSLPVSEVVFSNESAS 60

Query: 61  FSSLLQLSIRNLRFLTTTSPKPLFLVTPFHESHVQAAIVCARKKGLQVRVRSGGHDYEGL 120
           FSSLL+ SIRNLRFL+T+SPKPLFLVTPFHESHVQAAIVCA++  LQVRV SGGHDYEGL
Sbjct: 61  FSSLLKHSIRNLRFLSTSSPKPLFLVTPFHESHVQAAIVCAKENDLQVRVLSGGHDYEGL 120

Query: 121 SYV-SSQAPFIVIDLINLSSIIIDIQTETALVGTGATLGELYYRIAKKSPTHGFPAGSCP 180
           SY+ SSQAPFIVIDLINL SI I+I+TETALV TGAT+GELYYRIAKKSP HGFPAGSCP
Sbjct: 121 SYLSSSQAPFIVIDLINLRSIDINIKTETALVETGATIGELYYRIAKKSPIHGFPAGSCP 180

Query: 181 TVGVGGHISGGGFGTLFRKYGLAADNVIDAKIVDFNGRIMDRNSMGEDLFWAIRGGGGAS 240
           TVGVGGHISGGGFGTLFRKYGLAADNVIDAKIVDFNGRIMDRN+MGEDLFWAIRGGGGAS
Sbjct: 181 TVGVGGHISGGGFGTLFRKYGLAADNVIDAKIVDFNGRIMDRNTMGEDLFWAIRGGGGAS 240

Query: 241 FGVILSWKLKLVSVPSIVTVFNVQRTLEQGATQLFQKWQTIGHKLHQDIFLHVTTKVTN- 300
           FGVILSWKLKLVS+PSIVT FNVQRTL QGAT LFQKWQ I HKL QD+FLHVTTKV + 
Sbjct: 241 FGVILSWKLKLVSLPSIVTTFNVQRTLNQGATHLFQKWQQIAHKLDQDLFLHVTTKVIDQ 300

Query: 301 ---FPSKKTIRLSFTSLFLGPIERLIPLMKTHFPELGLKSIDCTEMSWIQSVLFFADLPT 360
                + KT+ LSFTSLFLG I+ LIPLM THFPELGLK   CTEM+WIQS+LFFAD  T
Sbjct: 301 KMTKTTSKTLSLSFTSLFLGSIDSLIPLMDTHFPELGLKRYQCTEMNWIQSILFFADFST 360

Query: 361 ETPLEVLMERSLPVSESFFTAKSDYVTSPISQNGLEGLWTKLLEEEKSELILTPYGGKMS 420
           ++PLE+LM+R LP + S F AKSDYVTSPISQ+GLEGLW KLLE E SELI TPYGGKMS
Sbjct: 361 KSPLEILMQR-LPSTRSSFIAKSDYVTSPISQSGLEGLWAKLLENENSELIFTPYGGKMS 420

Query: 421 QISESQIPFPHRKGSIFGIQYLATWDNANETEKHLSWIREVYTYMEPYVSKSPRAAYLNY 480
           QISE + PFPHRKGSIFG+QYLATWD++NE E+HL WIREVY+YMEPYVS +PR  YLNY
Sbjct: 421 QISELETPFPHRKGSIFGVQYLATWDDSNENERHLRWIREVYSYMEPYVSMAPRGTYLNY 480

Query: 481 RDLDLGRNYGRNTSYEQAKVWGLKYFSDNFERLVKVKTKVDPSNFFWNEQSIPLLYHYED 540
           RDLDLGRNYGRNTSYE+AKVWGLKYF  NFERLVKVKTK DP NFFWNEQSIPLLY+ ED
Sbjct: 481 RDLDLGRNYGRNTSYEEAKVWGLKYFKGNFERLVKVKTKFDPLNFFWNEQSIPLLYNCED 540

Query: 541 DTRIAKVYSGLDFEIVQER 552
           + RI +VYS L+ E VQER
Sbjct: 541 EARICEVYSDLNSENVQER 558

BLAST of CaUC03G058350 vs. ExPASy TrEMBL
Match: A0A6J1GQY4 (berberine bridge enzyme-like 15 OS=Cucurbita moschata OX=3662 GN=LOC111456716 PE=3 SV=1)

HSP 1 Score: 863.2 bits (2229), Expect = 5.8e-247
Identity = 439/562 (78.11%), Postives = 482/562 (85.77%), Query Frame = 0

Query: 1   MIWLRRIYLFLLFCVTLIWLC--SCKS----LQENFLECFNSTSYSKHSIPISEVVYTNE 60
           M+  RRI L L F     W+C  SC S    +QENF+ECFNST+YSKHS+P+SEVV++NE
Sbjct: 1   MVHWRRIQLLLFF-----WICSSSCSSSSAFVQENFIECFNSTTYSKHSLPVSEVVFSNE 60

Query: 61  SASFSSLLQLSIRNLRFLTTTSPKPLFLVTPFHESHVQAAIVCARKKGLQVRVRSGGHDY 120
           SASFSSLL+ SIRNLRFL+T+SPKPLFLVTPFHESHVQAAIVCA++  LQVRV SGGHDY
Sbjct: 61  SASFSSLLKHSIRNLRFLSTSSPKPLFLVTPFHESHVQAAIVCAKENDLQVRVLSGGHDY 120

Query: 121 EGLSYV-SSQAPFIVIDLINLSSIIIDIQTETALVGTGATLGELYYRIAKKSPTHGFPAG 180
           EGLSY+ SSQAPFIVIDLINL SI I+I+TETA V TGATLGELYYRIAKKSP HGFPAG
Sbjct: 121 EGLSYLSSSQAPFIVIDLINLRSIDINIETETASVETGATLGELYYRIAKKSPIHGFPAG 180

Query: 181 SCPTVGVGGHISGGGFGTLFRKYGLAADNVIDAKIVDFNGRIMDRNSMGEDLFWAIRGGG 240
           SCPTVGVGGHISGGGFGTLFRKYGLAADNVIDAKIVDFNGRIMDRN+M EDLFWAIRGGG
Sbjct: 181 SCPTVGVGGHISGGGFGTLFRKYGLAADNVIDAKIVDFNGRIMDRNTMEEDLFWAIRGGG 240

Query: 241 GASFGVILSWKLKLVSVPSIVTVFNVQRTLEQGATQLFQKWQTIGHKLHQDIFLHVTTKV 300
           GASFGVILSWKLKLVS+PSIVT FNVQRTL QG T LF KWQ I HKL+QD+FLHVTTKV
Sbjct: 241 GASFGVILSWKLKLVSLPSIVTTFNVQRTLNQGGTHLFLKWQEIAHKLNQDLFLHVTTKV 300

Query: 301 TN----FPSKKTIRLSFTSLFLGPIERLIPLMKTHFPELGLKSIDCTEMSWIQSVLFFAD 360
            +      + KT+ LSFTSLFLG I+ LIPLM THFPELGLK   CTEM+WIQSVLFFAD
Sbjct: 301 IDQKMTKTTSKTLSLSFTSLFLGSIDTLIPLMDTHFPELGLKRYQCTEMNWIQSVLFFAD 360

Query: 361 LPTETPLEVLMERSLPVSESFFTAKSDYVTSPISQNGLEGLWTKLLEEEKSELILTPYGG 420
             T++PLE+LM+R LP   S F AKSDYVTSPISQ+GLEGLW KLLE + SELI TPYGG
Sbjct: 361 FSTKSPLEILMQR-LPSIRSSFIAKSDYVTSPISQSGLEGLWAKLLENDNSELIFTPYGG 420

Query: 421 KMSQISESQIPFPHRKGSIFGIQYLATWDNANETEKHLSWIREVYTYMEPYVSKSPRAAY 480
           KMSQISE + PFPHRKGSIFG+QYLATWD++NE E+HL WIREVY+YMEPYVS +PR  Y
Sbjct: 421 KMSQISELETPFPHRKGSIFGVQYLATWDDSNENERHLRWIREVYSYMEPYVSMAPRGTY 480

Query: 481 LNYRDLDLGRNYGRNTSYEQAKVWGLKYFSDNFERLVKVKTKVDPSNFFWNEQSIPLLYH 540
           LNYRDLDLGRNYGRNTSYE+AKVWGLKYF  NFERLVKVKTK DP NFFWNEQSIPLLY+
Sbjct: 481 LNYRDLDLGRNYGRNTSYEEAKVWGLKYFKGNFERLVKVKTKFDPLNFFWNEQSIPLLYN 540

Query: 541 YEDDTRIAKVYSGLDFEIVQER 552
            ED+ RI +VYS LD   VQER
Sbjct: 541 CEDEARICEVYSDLDSANVQER 556

BLAST of CaUC03G058350 vs. TAIR 10
Match: AT2G34790.1 (FAD-binding Berberine family protein )

HSP 1 Score: 537.7 bits (1384), Expect = 1.1e-152
Identity = 267/522 (51.15%), Postives = 361/522 (69.16%), Query Frame = 0

Query: 12  LFCVTLIWL---CSCKSLQENFLECFNSTSYSKHSIPISEVVYTNESASFSSLLQLSIRN 71
           LF VTL+ +    S  +LQ++F++C    S     I  S       +  F   L+ + +N
Sbjct: 12  LFLVTLLLISVPLSSSTLQQDFVKCLVDNSDVSFPITASFFSPDQNATLFKEELESTAQN 71

Query: 72  LRFLTTTSPKPLFLVTPFHESHVQAAIVCARKKGLQVRVRSGGHDYEGLSYVS-SQAPFI 131
           LR+LT ++PKP+F+  P +E+HVQAA+VCA+K  L +R+RSGGHDYEGLS+V+  + PF+
Sbjct: 72  LRYLTPSNPKPVFIFEPLYETHVQAAVVCAKKLQLHLRLRSGGHDYEGLSFVAEDETPFV 131

Query: 132 VIDLINLSSIIIDIQTETALVGTGATLGELYYRIAKKSPTHGFPAGSCPTVGVGGHISGG 191
           ++DL  L  + +D+ + +A    GAT+GE+YYRI +KS THGFPAG C ++G+GGH+ GG
Sbjct: 132 IVDLSKLRQVDVDLDSNSAWAHAGATIGEVYYRIQEKSQTHGFPAGLCSSLGIGGHLVGG 191

Query: 192 GFGTLFRKYGLAADNVIDAKIVDFNGRIMDRNSMGEDLFWAIRGGGGASFGVILSWKLKL 251
            +G++ RK+GL ADNV+DA+IVD NG+I+DR +MGED+FWAIRGGGG SFGVIL+WK+KL
Sbjct: 192 AYGSMMRKFGLGADNVLDARIVDANGQILDRAAMGEDVFWAIRGGGGGSFGVILAWKIKL 251

Query: 252 VSVPSIVTVFNVQRTLEQGATQLFQKWQTIGHKLHQDIFLHV---TTKVTNFPSKKTIRL 311
           V VP+ VTVF V +TLEQ  T++  KW+ I  KL  D+F+ V       T  P  +TI +
Sbjct: 252 VPVPATVTVFTVTKTLEQDGTKVLYKWEQIADKLDDDLFIRVIISPASKTTKPGNRTISM 311

Query: 312 SFTSLFLGPIERLIPLMKTHFPELGLKSIDCTEMSWIQSVLFFADLPTETPLEVLMERSL 371
           S+ + FLG   RL+ +M+  FPELGL   DCTEMSWI+SV++ A  P     E L+    
Sbjct: 312 SYQAQFLGDSNRLLQVMQKSFPELGLTKKDCTEMSWIKSVMYIAGFPNSAAPEALL-AGK 371

Query: 372 PVSESFFTAKSDYVTSPISQNGLEGLWTKLLEEEKSELILTPYGGKMSQISESQIPFPHR 431
            + ++ F AKSD+V  PI   GLEGLW + LEE+    I  PYGG MS+ISES+IPFPHR
Sbjct: 372 SLFKNHFKAKSDFVKEPIPVEGLEGLWERFLEEDSPLTIWNPYGGMMSRISESEIPFPHR 431

Query: 432 KGSIFGIQYLATW-DNANETEKHLSWIREVYTYMEPYVSKSPRAAYLNYRDLDLGRNYGR 491
            G++F IQ+L+TW D     E+H+ WIRE+Y+YME YVSK+PR AY+NYRDLDLG N G 
Sbjct: 432 NGTLFKIQWLSTWQDGKVSEERHMKWIREMYSYMEQYVSKNPRQAYVNYRDLDLGTNEGE 491

Query: 492 NTSYEQAKVWGLKYFSDNFERLVKVKTKVDPSNFFWNEQSIP 526
                 A+ WG KY+  NFERLVK+K + DP NFF +EQS+P
Sbjct: 492 T----DAREWGAKYYKGNFERLVKIKGEFDPDNFFRHEQSVP 528

BLAST of CaUC03G058350 vs. TAIR 10
Match: AT5G44400.1 (FAD-binding Berberine family protein )

HSP 1 Score: 535.8 bits (1379), Expect = 4.1e-152
Identity = 264/523 (50.48%), Postives = 364/523 (69.60%), Query Frame = 0

Query: 7   IYLFLLFCVTLIWLCSCKSLQENFLECFNSTSYSKHSIPISEVVYTNESASFSSLLQLSI 66
           +  F L+ +T     S  SLQ+ F+ C    ++    +  +    T   + FS +L+ + 
Sbjct: 14  VLYFSLYTITP--TSSLASLQDQFINCVQRNTHVYFPLEKTFFAPTKNVSMFSQVLESTA 73

Query: 67  RNLRFLTTTSPKPLFLVTPFHESHVQAAIVCARKKGLQVRVRSGGHDYEGLSYVSS-QAP 126
           +NLRFL  + PKP F+ +P HESHVQA+I+C++K  + +RVRSGGHDYEGLSYVS    P
Sbjct: 74  QNLRFLKKSMPKPGFIFSPIHESHVQASIICSKKLRMHLRVRSGGHDYEGLSYVSQIDKP 133

Query: 127 FIVIDLINLSSIIIDIQTETALVGTGATLGELYYRIAKKSPTHGFPAGSCPTVGVGGHIS 186
           FI++DL  +  + I+IQ  +A V +GAT+GELYYRIA+KS  HGFPAG C ++G+GGHI+
Sbjct: 134 FILMDLSKMRQVNINIQDNSAWVQSGATVGELYYRIAEKSKVHGFPAGLCSSLGIGGHIT 193

Query: 187 GGGFGTLFRKYGLAADNVIDAKIVDFNGRIMDRNSMGEDLFWAIRGGGGASFGVILSWKL 246
           GG +G++ RKYGL ADNV+DAKIVD NG+++DR +MGED FWAIRGG G SFG+IL+WK+
Sbjct: 194 GGAYGSMMRKYGLGADNVLDAKIVDANGKLLDRAAMGEDTFWAIRGGAGGSFGIILAWKI 253

Query: 247 KLVSVPSIVTVFNVQRTLEQG-ATQLFQKWQTIGHKLHQDIFLHVTTKVTNFPSKKTIRL 306
           KLV VP  VTVF V +TL+Q    ++  KWQ +  KL +++F+ V   V      KT+  
Sbjct: 254 KLVPVPKTVTVFTVTKTLQQDVGNKIISKWQRVADKLVEELFIRVLFNVAGTGGNKTVTT 313

Query: 307 SFTSLFLGPIERLIPLMKTHFPELGLKSIDCTEMSWIQSVLFFADLPTETPLEVLMERSL 366
           S+ +LFLG    L+ +MK  FPELGL   DC EMSW++S+ + +  PT TP  VL++   
Sbjct: 314 SYNALFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLESIAYISGFPTHTPTNVLLQGKS 373

Query: 367 PVSESFFTAKSDYVTSPISQNGLEGLWTKLLEEEKSELILTPYGGKMSQISESQIPFPHR 426
           P  +  F AKSD+V +PI ++GL+G++ KLL+E+   +I  PYGG M++I ESQIPFPHR
Sbjct: 374 PFPKVSFKAKSDFVKTPIPESGLQGIFKKLLKEDIPLMIWNPYGGMMAKIPESQIPFPHR 433

Query: 427 KGSIFGIQYLATW-DNANETEKHLSWIREVYTYMEPYVSKSPRAAYLNYRDLDLGRN-YG 486
           KG +F +QY+ +W D+     +H++WIR++Y+YM PYVS +PR AY+NYRDLDLGRN   
Sbjct: 434 KGVLFKVQYVTSWLDSDKRPSRHINWIRDLYSYMTPYVSSNPREAYVNYRDLDLGRNTKD 493

Query: 487 RNTSYEQAKVWGLKYFSDNFERLVKVKTKVDPSNFFWNEQSIP 526
             T  +QA+VWG  YF +NF RL+ +K KVDP NFF +EQSIP
Sbjct: 494 VKTCIKQAQVWGANYFKNNFNRLMMIKAKVDPENFFRHEQSIP 534

BLAST of CaUC03G058350 vs. TAIR 10
Match: AT1G30700.1 (FAD-binding Berberine family protein )

HSP 1 Score: 535.0 bits (1377), Expect = 7.0e-152
Identity = 265/525 (50.48%), Postives = 366/525 (69.71%), Query Frame = 0

Query: 9   LFLLFCVTLIWLCSCKSL-QENFLECFNSTSYSKHSIPISEVVYTNESASFSSLLQLSIR 68
           + +LF V  IW  S  S   E F +C  S S  KH  PIS  ++ + + S+SS+LQ +IR
Sbjct: 6   ILVLFFVVFIWQSSSSSANSETFTQCLTSNSDPKH--PISPAIFFSGNGSYSSVLQANIR 65

Query: 69  NLRFLTTTSPKPLFLVTPFHESHVQAAIVCARKKGLQVRVRSGGHDYEGLSYVS-SQAPF 128
           NLRF TT++PKP  ++   HESHVQAAI C ++  LQ+++RSGGHDY+GLSYV+ S  PF
Sbjct: 66  NLRFNTTSTPKPFLIIAATHESHVQAAITCGKRHNLQMKIRSGGHDYDGLSYVTYSGKPF 125

Query: 129 IVIDLINLSSIIIDIQTETALVGTGATLGELYYRIAKKSPTHGFPAGSCPTVGVGGHISG 188
            V+D+ NL S+ +D+ ++TA V TGA LGE+YY I +KS T  +PAG CPTVGVGGHISG
Sbjct: 126 FVLDMFNLRSVDVDVASKTAWVQTGAILGEVYYYIWEKSKTLAYPAGICPTVGVGGHISG 185

Query: 189 GGFGTLFRKYGLAADNVIDAKIVDFNGRIMDRNSMGEDLFWAIRGGGGASFGVILSWKLK 248
           GG+G + RKYGL  DN IDA++VD NG+I+DR  MGEDL+WAI GGGG S+GV+L++K+ 
Sbjct: 186 GGYGNMMRKYGLTVDNTIDARMVDVNGKILDRKLMGEDLYWAINGGGGGSYGVVLAYKIN 245

Query: 249 LVSVPSIVTVFNVQRTLEQGATQLFQKWQTIGHKLHQDIFLHVTTKVTN--FPSKKTIRL 308
           LV VP  VTVF + RTLEQ AT +  +WQ +  KL  ++F+     V N    S+KT+R 
Sbjct: 246 LVEVPENVTVFRISRTLEQNATDIIHRWQQVAPKLPDELFIRTVIDVVNGTVSSQKTVRT 305

Query: 309 SFTSLFLGPIERLIPLMKTHFPELGLKSIDCTEMSWIQSVLFFADLPTETPLEVLMERSL 368
           +F ++FLG    L+ ++   FPELGL   DCTE SWIQSVLF+ ++   +   +L++R+ 
Sbjct: 306 TFIAMFLGDTTTLLSILNRRFPELGLVRSDCTETSWIQSVLFWTNIQVGSSETLLLQRNQ 365

Query: 369 PVSESFFTAKSDYVTSPISQNGLEGLWTKLLEEEKSELILTPYGGKMSQISESQIPFPHR 428
           PV  ++   KSDYV  PIS+ GLE +W K++E E   +   PYGG+M +IS +  PFP+R
Sbjct: 366 PV--NYLKRKSDYVREPISRTGLESIWKKMIELEIPTMAFNPYGGEMGRISSTVTPFPYR 425

Query: 429 KGSIFGIQYLATWDNANETEKHLSWIREVYTYMEPYVSKSPRAAYLNYRDLDLGRN--YG 488
            G+++ IQY A W +   T++++   R++Y +M P+VSK+PR ++ NYRD+DLG N   G
Sbjct: 426 AGNLWKIQYGANWRDETLTDRYMELTRKLYQFMTPFVSKNPRQSFFNYRDVDLGINSHNG 485

Query: 489 RNTSYEQAKVWGLKYFSDNFERLVKVKTKVDPSNFFWNEQSIPLL 528
           + +SY + K +G KYF+ NFERLVK+KT+VD  NFF NEQSIP+L
Sbjct: 486 KISSYVEGKRYGKKYFAGNFERLVKIKTRVDSGNFFRNEQSIPVL 526

BLAST of CaUC03G058350 vs. TAIR 10
Match: AT5G44390.1 (FAD-binding Berberine family protein )

HSP 1 Score: 534.3 bits (1375), Expect = 1.2e-151
Identity = 267/523 (51.05%), Postives = 362/523 (69.22%), Query Frame = 0

Query: 11  LLFCVTLIWLCSCKSLQENFLECFNSTSYSKHSIPISEVVYTNESASFSSLLQLSIRNLR 70
           L F    I L S  SLQ++F++C    +  + ++  +       ++ F+ +L+ + +N R
Sbjct: 18  LYFSFYTITLTSSTSLQDDFIKCLYRNTNVRFTLDKTFFTPERNASIFTEVLESTAQNQR 77

Query: 71  FLTTTSPKPLFLVTPFHESHVQAAIVCARKKGLQVRVRSGGHDYEGLSYVSS-QAPFIVI 130
           +LT T PKP F+  P HESHVQA+++C++K  +  RVRSGGHDYEG+SYVS  + PF++I
Sbjct: 78  YLTKTMPKPGFIFKPVHESHVQASVICSKKLEIHFRVRSGGHDYEGVSYVSQIEKPFVLI 137

Query: 131 DLINLSSIIIDIQTETALVGTGATLGELYYRIAKKSPTHGFPAGSCPTVGVGGHISGGGF 190
           DL  L  I +DI+  +A V  GAT+GELYYRIA+KS  HGFPAG  P++G+GGHI+GG +
Sbjct: 138 DLSKLRQINVDIKDTSAWVEAGATVGELYYRIAEKSKFHGFPAGVYPSLGIGGHITGGAY 197

Query: 191 GTLFRKYGLAADNVIDAKIVDFNGRIMDRNSMGEDLFWAIRGGGGASFGVILSWKLKLVS 250
           G+L RKYGLAADNV+DAKIVD NG+++DR SMGEDLFWAIRGG G SFG+ILSWK+KLV 
Sbjct: 198 GSLMRKYGLAADNVLDAKIVDANGKLLDRASMGEDLFWAIRGGSGGSFGIILSWKIKLVP 257

Query: 251 VPSIVTVFNVQRTLEQGAT-QLFQKWQTIGHKLHQDIFLHVTTKVTNFPSKKTIRLSFTS 310
           VP  +TVF V +T EQ  + ++  KWQ I   L  ++FL V   V+   + KT+ +++  
Sbjct: 258 VPETLTVFTVTKTFEQDRSFKILSKWQEIADNLVDELFLRVFFTVSGNKANKTVTMAYIG 317

Query: 311 LFLGPIERLIPLMKTHFPELGLKSIDCTEMSWIQSVLFFADLPTE--TPLEVLMERSLPV 370
            FLG    L+ +MK  FPELGL   DC EMSWI S+++ +  PT    P+E+L++   P+
Sbjct: 318 QFLGEKGTLMEVMKKDFPELGLTQKDCIEMSWIDSIIYNSGFPTNPPPPIEILLQAKSPI 377

Query: 371 SESFFTAKSDYVTSPISQNGLEGLWTKLLEEEKSELILTPYGGKMSQISESQIPFPHRKG 430
            + +F  KSD+   PI   GLEG++ KLLEE+ + +I TPYGGKM +I ES+IPFPHR G
Sbjct: 378 GKVYFKGKSDFAKKPIPVLGLEGMFKKLLEEDAALVIWTPYGGKMDKIPESEIPFPHRNG 437

Query: 431 SIFGIQYLATW-DNANETEKHLSWIREVYTYMEPYVSKSPRAAYLNYRDLDLGRNYGRNT 490
           + F IQY  +W D+     +   WIRE+Y YM PYVS +PR AY+NYRDLDLG+N   + 
Sbjct: 438 TNFMIQYYRSWSDSEKRPNRRTKWIRELYGYMTPYVSSNPRQAYVNYRDLDLGQNKDNSK 497

Query: 491 S-YEQAKVWGLKYFSDNFERLVKVKTKVDPSNFFWNEQSIPLL 528
           S + +AK+WG  YF DNF RLV++K+KVDP NFF +EQSIP L
Sbjct: 498 SNFIEAKIWGANYFKDNFNRLVRIKSKVDPDNFFRHEQSIPTL 540

BLAST of CaUC03G058350 vs. TAIR 10
Match: AT1G30760.1 (FAD-binding Berberine family protein )

HSP 1 Score: 530.4 bits (1365), Expect = 1.7e-150
Identity = 266/524 (50.76%), Postives = 365/524 (69.66%), Query Frame = 0

Query: 13  FCVTLIWL------CSCKSLQENFLECFNSTSYSKHSIPISEVVYTNESASFSSLLQLSI 72
           F VTL+ L       S  ++Q++F+ C    S +   +  S   +   ++SF   L+ S 
Sbjct: 12  FLVTLLLLSLSYIPLSFSTIQQDFVMCLVDNSDASFPMDSSFFTHDLNASSFKLALETSA 71

Query: 73  RNLRFLTTTSPKPLFLVTPFHESHVQAAIVCARKKGLQVRVRSGGHDYEGLSYVSS-QAP 132
           +NLR+L  ++PKP F+  P +E+HVQAA++CA+K  L +R+RSGGHDYEGLSYVS  +  
Sbjct: 72  QNLRYLMPSNPKPEFIFEPLYETHVQAAVLCAKKLKLHLRLRSGGHDYEGLSYVSEMETA 131

Query: 133 FIVIDLINLSSIIIDIQTETALVGTGATLGELYYRIAKKSPTHGFPAGSCPTVGVGGHIS 192
           F+++DL  L  I +DI++ +A V  GA++GE+YYRI +KS  HGFPAG C ++G+GGHI 
Sbjct: 132 FVIVDLSKLRQISVDIESNSAWVHAGASIGEVYYRIQEKSKIHGFPAGLCTSLGIGGHII 191

Query: 193 GGGFGTLFRKYGLAADNVIDAKIVDFNGRIMDRNSMGEDLFWAIRGGGGASFGVILSWKL 252
           GG +G++ RK+GL ADNV+DA+IVD +G+I++R +MGED+FWAIRGGGG SFGVIL+WK+
Sbjct: 192 GGAYGSMMRKFGLGADNVLDARIVDADGKILNRAAMGEDVFWAIRGGGGGSFGVILAWKI 251

Query: 253 KLVSVPSIVTVFNVQRTLEQGATQLFQKWQTIGHKLHQDIFLHVTTKVTNFPSK---KTI 312
           KLV VP IVTVF V RTLEQ  T+L  KWQ +  KL +D+F+ V  + T+   K   +TI
Sbjct: 252 KLVPVPEIVTVFTVTRTLEQDGTKLLYKWQQVADKLDEDLFIRVIIQPTSKTPKSKERTI 311

Query: 313 RLSFTSLFLGPIERLIPLMKTHFPELGLKSIDCTEMSWIQSVLFFADLPTETPLEVLMER 372
             S+   FLG   RL+ +M+  FP+LGL   DC E SWI+SV++ A  P+  P E L++ 
Sbjct: 312 STSYQGQFLGDANRLLQVMQRSFPQLGLTKKDCLETSWIKSVMYIAGFPSTAPSEALLD- 371

Query: 373 SLPVSESFFTAKSDYVTSPISQNGLEGLWTKLLEEEKSELILTPYGGKMSQISESQIPFP 432
              + +++F AKSDYV  PI   GLEGLW KLLEE+    I  PYGG M++I E++ PFP
Sbjct: 372 GKSLFKNYFKAKSDYVEEPIPVEGLEGLWEKLLEEDSPLTIWNPYGGMMAKIPETETPFP 431

Query: 433 HRKGSIFGIQYLATWDNANETE-KHLSWIREVYTYMEPYVSKSPRAAYLNYRDLDLGRNY 492
           HR G++F IQ+L  W +   +E KH+ W+RE+Y+YME YVSKSPR+AY+NYRDLDLG N 
Sbjct: 432 HRSGTLFKIQWLTLWQDGKTSEAKHMGWMREMYSYMEQYVSKSPRSAYVNYRDLDLGMN- 491

Query: 493 GRNTSYEQAKVWGLKYFSDNFERLVKVKTKVDPSNFFWNEQSIP 526
           G+ +    A+ WG +YF  NFERLV++K K DP NFF +EQSIP
Sbjct: 492 GKGS---DAREWGNRYFKGNFERLVEIKAKFDPENFFRHEQSIP 530

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038895849.14.3e-28991.34berberine bridge enzyme-like 8 [Benincasa hispida][more]
XP_008445632.13.2e-28489.71PREDICTED: flavin-dependent oxidoreductase FOX5-like [Cucumis melo] >KAA0047127.... [more]
KAE8653305.18.2e-28087.75hypothetical protein Csa_023319 [Cucumis sativus][more]
XP_004135724.18.2e-28087.75berberine bridge enzyme-like 13 [Cucumis sativus][more]
XP_022140178.12.4e-25580.29berberine bridge enzyme-like 8 [Momordica charantia][more]
Match NameE-valueIdentityDescription
O647431.5e-15151.15Berberine bridge enzyme-like 15 OS=Arabidopsis thaliana OX=3702 GN=MEE23 PE=1 SV... [more]
Q9FKU85.7e-15150.48Berberine bridge enzyme-like 26 OS=Arabidopsis thaliana OX=3702 GN=At5g44400 PE=... [more]
Q9SA859.8e-15150.48Berberine bridge enzyme-like 8 OS=Arabidopsis thaliana OX=3702 GN=At1g30700 PE=2... [more]
Q9FKU91.7e-15051.05Berberine bridge enzyme-like 25 OS=Arabidopsis thaliana OX=3702 GN=At5g44390 PE=... [more]
Q93ZA32.4e-14950.76Berberine bridge enzyme-like 13 OS=Arabidopsis thaliana OX=3702 GN=At1g30760 PE=... [more]
Match NameE-valueIdentityDescription
A0A5A7TYX11.6e-28489.71Flavin-dependent oxidoreductase FOX5-like OS=Cucumis melo var. makuwa OX=1194695... [more]
A0A1S3BE111.6e-28489.71flavin-dependent oxidoreductase FOX5-like OS=Cucumis melo OX=3656 GN=LOC10348858... [more]
A0A6J1CHF01.2e-25580.29berberine bridge enzyme-like 8 OS=Momordica charantia OX=3673 GN=LOC111010911 PE... [more]
A0A6J1JUS47.1e-25379.61berberine bridge enzyme-like 15 OS=Cucurbita maxima OX=3661 GN=LOC111489931 PE=3... [more]
A0A6J1GQY45.8e-24778.11berberine bridge enzyme-like 15 OS=Cucurbita moschata OX=3662 GN=LOC111456716 PE... [more]
Match NameE-valueIdentityDescription
AT2G34790.11.1e-15251.15FAD-binding Berberine family protein [more]
AT5G44400.14.1e-15250.48FAD-binding Berberine family protein [more]
AT1G30700.17.0e-15250.48FAD-binding Berberine family protein [more]
AT5G44390.11.2e-15151.05FAD-binding Berberine family protein [more]
AT1G30760.11.7e-15050.76FAD-binding Berberine family protein [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (USVL246-FR2) v1
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR016169FAD-binding, type PCMH, subdomain 2GENE3D3.30.465.10coord: 139..525
e-value: 1.9E-170
score: 569.4
IPR012951Berberine/berberine-likePFAMPF08031BBEcoord: 468..525
e-value: 3.1E-22
score: 78.4
IPR016167FAD-binding, type PCMH, subdomain 1GENE3D3.30.43.10coord: 24..134
e-value: 1.7E-42
score: 145.6
IPR006094FAD linked oxidase, N-terminalPFAMPF01565FAD_binding_4coord: 79..216
e-value: 4.1E-27
score: 94.6
NoneNo IPR availableGENE3D3.40.462.20coord: 253..464
e-value: 1.9E-170
score: 569.4
NoneNo IPR availablePANTHERPTHR32448:SF121FAD-BINDING BERBERINE FAMILY PROTEINcoord: 27..526
NoneNo IPR availablePANTHERPTHR32448OS08G0158400 PROTEINcoord: 27..526
NoneNo IPR availablePROSITEPS51257PROKAR_LIPOPROTEINcoord: 1..23
score: 6.0
IPR016166FAD-binding domain, PCMH-typePROSITEPS51387FAD_PCMHcoord: 75..250
score: 18.873049
IPR036318FAD-binding, type PCMH-like superfamilySUPERFAMILY56176FAD-binding/transporter-associated domain-likecoord: 27..251

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CaUC03G058350.1CaUC03G058350.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0071949 FAD binding
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0050660 flavin adenine dinucleotide binding