CaUC03G057640 (gene) Watermelon (USVL246-FR2) v1

Overview
NameCaUC03G057640
Typegene
OrganismCitrullus amarus (Watermelon (USVL246-FR2) v1)
Descriptionprotease Do-like 5, chloroplastic
LocationCiama_Chr03: 8602941 .. 8606206 (+)
RNA-Seq ExpressionCaUC03G057640
SyntenyCaUC03G057640
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
AACAGTCATCTCAACCGCCAAGAAGATAACCGGATTGCTAGTCGAAGATTGGTTGAAGAAACAAAGTGGAAATCCATGGCGTTGGGCTCACTGGGAATTCGTGTTCTTCCAGTTTCAGCTTCCCCAAATCCGTCTGAAAATCCCTTACCCTTCACTTCCCGAAGAGCCATTGTGTTTGCCCCCACTGCTTTAATGGCTTCCCTGCTCGCTTTCCCCCTTCCCACTTACGCCGCTCTTCCCCAACTGCAAGACGACATTCCACAAGAAGAAGATCGAATCATTAACCTTTTCCAGGTCCCTCTCTTTCTCTGAATATAAATGTTGATTTCTTAAGTTTCAATTGAAATTGGGTCTTGCTTTATCTGTTTTGTTCTGTTGGTTCCCGATTGCAATGTAGGAAACTTCGCCTTCTGTCGTTTACATTAAGGACCTTGAAATAGCTAAAAACCCTCAAAACCATTCTGAAGAAGCCATACTTATTGAGGATGAAAATGCCAAGGTCAAAGGGACTGGTTCCGGCTTTGTTTGGGATAAATTTGGCCATATCGTAAGCCCTTCTCTTCTTTGATTCTTATAATTCTTGTTTTTCCCAACTCTTCTTCTAAGATGGATAATCCCCTAGAGAACTTACCTGTCTCAATTGTGCACAGCCTGAAACACCTTTGTTAGTTAGCTGAAGCATTTCTGCTTGTGACAGGTCACTAATTACCATGTTGTTTCTGCATTGGCTACTGATAATAGTGGATTACAGCGTTGTAAGGTTTTGTTCGTTAGTTACTGTGTTTTGGATTGTTGTGCATTCTTTGTGGATGATATGCTTGCAGTCTCTAGCCATGATTTCTCAAATTTGGGTTCTTCTGTAGGTAAATTTAGTGGATGTTAAAGGAAATGGAATCTATAAAGAAGCAAAGATTGTTGGTTTTGATCCGGAGTATGATCTAGCTGTTCTTAAGGTATTGAATCAAGCTTGCTTGTATTTTTCCTTTTCTTTTCCCGCTAGTTTTCTTTAAGCTTTTGGTTTGATTTGTTATGTGAAAATTATGTGCTGTCAATTCAGCGAATGGCTTCACTCAGTTTCGACATTCATGAAATCCCTCCTAAAATTGGTGTCCATATATAAATTCTATATCGATGTGTTAATTTAGTTCAAAAATCATGTATAGGACATTCCTATCTTTATTCCCTTCTAAAGGTCTCTGAATACAGATACAAGAGACGTATATAACACAGGCACAGTGACATGTCATTCTTTAAGAATCTAAGATACAAGTATAGAAAGATCACATTTATTAAAATATAAATCATTTTTGTACAACAGAAAAAATGAGTATGATATATTTTGATCTAAACTTTATTTTATTCAGCCTATATTATTTTGTGTCTATTTAGTATATTTAACAAGTATTTGATACGTATCTAACAAATGATGAACCTATATTGACTTTGTACAACTAGTGTCAAAACACATATCTATCATGTCAACAAGTGTTCAATATTTGTCCAACAAGTGTCGAAATATTGAAGTGTCTGAAACTTGTTGGACATGAATACGCTAGCCAAGTCAAAGTGTAAATACTTTTTTTTGTTCGCAAACAAGTAAAAACGATTCCCTTATGCTTTTTAGCATCATTCACAATAAGGTCGAAAAGCTACTAATATTTGGCTAATCAAAACTGATTAGTGTTTTTGGAAAACCACTGATGAATTTGTGAGATAAGAACTATGTTTGTGTCATATCGTATGTACATCAATCACAAATAACCTTGCAGAAATTATAGTATAGGGACTATGTATTTTTTGGGAAGAAAGGATCCTCAAATGGCTCTAGTAGTAACTTAACAAGGCAGAAAAGCCAGATCATTCCATTTATTTCGTCAACACCCAGCCATAGTTTCGAGAGGATTACCAGATTTGGGTAAATCATGTTTTGGTGATTTTCATCCCATTTTCTATTCCAAATATGGTGTGCAAATACAGCAGATTGGACAATCTATATTGTCAATCTCTTATGTATACCATGATTTGCTGAGTTTGAATTTTCCATGTAACATCCAGGTAGAACTTGAAGGACAAGAACTGAAGCCCATAGTCTTTGGTGCCTCTCGAAATCTACGTGTTGGTCAGAGCTGCTATGCCATTGGCAACCCTTTTGGTTATGAGAAGACACTAACAGCAGGGGTAAGGCATAAATAATGCTATTTCCTCCATTTAATTTAGGGAAACCCTCTCTGAGTTTTCCATTCCAAAGCTAAAACTAAATTCCATAGTGAAATACTTCTGTCTTACAATAAACGTGTCAATCATAGAAGGTGTGATATCCCTCATCTCACACAAATTTATGCTTTACCAAATAACTTGGATGAAGTAAGAGTTTCTCTAAGTGCCACTCTCAAGTTTCTTACTAGTGTTGTTTACCAAGTAACACGTCATATAGCAGATTGACTAAATAATTGAGGACCGTAAATCATTCAGGGTTTGGATAGCAGGTGATCAGCGGATTGGGTAGAGAAATTCCATCGCCAAATGGAAGGGCCATCAGGGGAGCTATTCAGACAGATGCTGCTATTAGTGCAGGTTCATGTTTCAACTACCTGAAATAAGCTTTTGCCCTCTCAAAACTATCATCTTTTCATGCTTTTCTTCCTCATTTTAAAGTTAAACTACTTTCATCACTCACATCGCAAGTGTAAGCCTTATAGTTAATTGATTCTACTGATCTTGATCTTGATCTTGGTCTTTCTATTCTATCTTTGGTTCATTGCTGCCCGAGTCATCCATTATAATAACTATCTAAACTTACCCACATTTTAATACATCAAAATTCTATAGGGAATTCAGGGGGGCCATTAGTTGACTCATACGGTCATGTTATTGGAGTCAACACAGCAACTTTCACTCGCAAAGGTGAAGGATCTCAATTTCTTCATCTACCTTAGTTTGTCAGAGGGAATATGAAAGCATAATGTTGCCCACTTTTGCTTTAAATGTGGCTGATTTATTTGCCTTAGCTTAGGAATTATTAAAACCAACTCAACTTTTTATAGCCACTTGGGAAAAAAAAAACATGTCTCCACAATAGTTAGAACGAACAAATAATGGTATTTTTTGTGGTTATTGAGTTCAAAGTTGTGTTTTTTCATGTTTGGTTATGCACTGCAGGAACTGGAATGTCTTCTGGTGTTAATTTTGCTATACCAATAGACACAGTTGTACGGACTGTGCCATACCTTATTGTATATGGAACACCTTACAGTGAGAGATTTTGA

mRNA sequence

AACAGTCATCTCAACCGCCAAGAAGATAACCGGATTGCTAGTCGAAGATTGGTTGAAGAAACAAAGTGGAAATCCATGGCGTTGGGCTCACTGGGAATTCGTGTTCTTCCAGTTTCAGCTTCCCCAAATCCGTCTGAAAATCCCTTACCCTTCACTTCCCGAAGAGCCATTGTGTTTGCCCCCACTGCTTTAATGGCTTCCCTGCTCGCTTTCCCCCTTCCCACTTACGCCGCTCTTCCCCAACTGCAAGACGACATTCCACAAGAAGAAGATCGAATCATTAACCTTTTCCAGGAAACTTCGCCTTCTGTCGTTTACATTAAGGACCTTGAAATAGCTAAAAACCCTCAAAACCATTCTGAAGAAGCCATACTTATTGAGGATGAAAATGCCAAGGTCAAAGGGACTGGTTCCGGCTTTGTTTGGGATAAATTTGGCCATATCGTCACTAATTACCATGTTGTTTCTGCATTGGCTACTGATAATAGTGGATTACAGCGTTGTAAGGTTTTGTTCGTAAATTTAGTGGATGTTAAAGGAAATGGAATCTATAAAGAAGCAAAGATTGTTGGTTTTGATCCGGAGTATGATCTAGCTGTTCTTAAGGTATTGAATCAAGCTTGCTTCGAATGGCTTCACTCAGTTTCGACATTCATGAAATCCCTCCTAAAATTGGTAGAACTTGAAGGACAAGAACTGAAGCCCATAGTCTTTGGTGCCTCTCGAAATCTACGTGTTGGTCAGAGCTGCTATGCCATTGGCAACCCTTTTGGTTATGAGAAGACACTAACAGCAGGGGTGATCAGCGGATTGGGTAGAGAAATTCCATCGCCAAATGGAAGGGCCATCAGGGGAGCTATTCAGACAGATGCTGCTATTAGTGCAGGAACTGGAATGTCTTCTGGTGTTAATTTTGCTATACCAATAGACACAGTTGTACGGACTGTGCCATACCTTATTGTATATGGAACACCTTACAGTGAGAGATTTTGA

Coding sequence (CDS)

AACAGTCATCTCAACCGCCAAGAAGATAACCGGATTGCTAGTCGAAGATTGGTTGAAGAAACAAAGTGGAAATCCATGGCGTTGGGCTCACTGGGAATTCGTGTTCTTCCAGTTTCAGCTTCCCCAAATCCGTCTGAAAATCCCTTACCCTTCACTTCCCGAAGAGCCATTGTGTTTGCCCCCACTGCTTTAATGGCTTCCCTGCTCGCTTTCCCCCTTCCCACTTACGCCGCTCTTCCCCAACTGCAAGACGACATTCCACAAGAAGAAGATCGAATCATTAACCTTTTCCAGGAAACTTCGCCTTCTGTCGTTTACATTAAGGACCTTGAAATAGCTAAAAACCCTCAAAACCATTCTGAAGAAGCCATACTTATTGAGGATGAAAATGCCAAGGTCAAAGGGACTGGTTCCGGCTTTGTTTGGGATAAATTTGGCCATATCGTCACTAATTACCATGTTGTTTCTGCATTGGCTACTGATAATAGTGGATTACAGCGTTGTAAGGTTTTGTTCGTAAATTTAGTGGATGTTAAAGGAAATGGAATCTATAAAGAAGCAAAGATTGTTGGTTTTGATCCGGAGTATGATCTAGCTGTTCTTAAGGTATTGAATCAAGCTTGCTTCGAATGGCTTCACTCAGTTTCGACATTCATGAAATCCCTCCTAAAATTGGTAGAACTTGAAGGACAAGAACTGAAGCCCATAGTCTTTGGTGCCTCTCGAAATCTACGTGTTGGTCAGAGCTGCTATGCCATTGGCAACCCTTTTGGTTATGAGAAGACACTAACAGCAGGGGTGATCAGCGGATTGGGTAGAGAAATTCCATCGCCAAATGGAAGGGCCATCAGGGGAGCTATTCAGACAGATGCTGCTATTAGTGCAGGAACTGGAATGTCTTCTGGTGTTAATTTTGCTATACCAATAGACACAGTTGTACGGACTGTGCCATACCTTATTGTATATGGAACACCTTACAGTGAGAGATTTTGA

Protein sequence

NSHLNRQEDNRIASRRLVEETKWKSMALGSLGIRVLPVSASPNPSENPLPFTSRRAIVFAPTALMASLLAFPLPTYAALPQLQDDIPQEEDRIINLFQETSPSVVYIKDLEIAKNPQNHSEEAILIEDENAKVKGTGSGFVWDKFGHIVTNYHVVSALATDNSGLQRCKVLFVNLVDVKGNGIYKEAKIVGFDPEYDLAVLKVLNQACFEWLHSVSTFMKSLLKLVELEGQELKPIVFGASRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTDAAISAGTGMSSGVNFAIPIDTVVRTVPYLIVYGTPYSERF
Homology
BLAST of CaUC03G057640 vs. NCBI nr
Match: XP_038893976.1 (protease Do-like 5, chloroplastic [Benincasa hispida])

HSP 1 Score: 478.0 bits (1229), Expect = 6.5e-131
Identity = 258/330 (78.18%), Postives = 266/330 (80.61%), Query Frame = 0

Query: 26  MALGSLGIRVLPVSASPNPSENPLPFTSRRAIVFAPTALMASLLAFPLPTYAALPQLQDD 85
           MALGSLGIR+LP+S+ PN +ENPLP TSRRAIVFAPTALMASLLAFP+PTYAALPQLQDD
Sbjct: 1   MALGSLGIRLLPISSPPNSAENPLPITSRRAIVFAPTALMASLLAFPVPTYAALPQLQDD 60

Query: 86  IPQEEDRIINLFQETSPSVVYIKDLEIAKNPQNHSEEAILIEDENAKVKGTGSGFVWDKF 145
           IPQEEDRI+ LFQETSPSVVYIKDLE+AKNPQN S      EDENAKVKGTGSGFVWDKF
Sbjct: 61  IPQEEDRIVALFQETSPSVVYIKDLEVAKNPQNPSG-----EDENAKVKGTGSGFVWDKF 120

Query: 146 GHIVTNYHVVSALATDNSGLQRCKVLFVNLVDVKGNGIYKEAKIVGFDPEYDLAVLKVLN 205
           GHIVTNYHVVSALATDNSGLQRCK   VNLVDVKGNGIYKEAKIVGFDPEYDLAVLK   
Sbjct: 121 GHIVTNYHVVSALATDNSGLQRCK---VNLVDVKGNGIYKEAKIVGFDPEYDLAVLK--- 180

Query: 206 QACFEWLHSVSTFMKSLLKLVELEGQELKPIVFGASRNLRVGQSCYAIGNPFGYEKTLTA 265
                               VELEG ELKPIVFG SRNLRVGQSCYAIGNPFGYEKTLTA
Sbjct: 181 --------------------VELEGHELKPIVFGTSRNLRVGQSCYAIGNPFGYEKTLTA 240

Query: 266 GVISGLGREIPSPNGRAIRGAIQTDAAISA-------------------------GTGMS 325
           GVISGLGREIPSPNGRAIRGAIQTDAAISA                         GTGMS
Sbjct: 241 GVISGLGREIPSPNGRAIRGAIQTDAAISAGNSGGPLVDSYGHVIGVNTATFTRKGTGMS 299

Query: 326 SGVNFAIPIDTVVRTVPYLIVYGTPYSERF 331
           SGVNFAIPIDTV+RTVPYLIVYGTPYSERF
Sbjct: 301 SGVNFAIPIDTVIRTVPYLIVYGTPYSERF 299

BLAST of CaUC03G057640 vs. NCBI nr
Match: XP_022927238.1 (protease Do-like 5, chloroplastic isoform X1 [Cucurbita moschata])

HSP 1 Score: 471.5 bits (1212), Expect = 6.1e-129
Identity = 255/331 (77.04%), Postives = 269/331 (81.27%), Query Frame = 0

Query: 26  MALGSLGIRVLPVSASPNPSENPLPFTSRRAIVFAPTALMASLLAFPLPTYAALPQLQDD 85
           MALGSLGIR+LPVS+ PN SE  LPFTSRRAIVFAPTALMASLLAFP+P++AALPQLQD+
Sbjct: 1   MALGSLGIRLLPVSSPPNSSEISLPFTSRRAIVFAPTALMASLLAFPVPSFAALPQLQDE 60

Query: 86  IPQEEDRIINLFQETSPSVVYIKDLEIAKNPQNHSEEAILIE-DENAKVKGTGSGFVWDK 145
           +PQEEDRI+ LFQETSPSVVYIK+LEIAK PQN SEEA+LIE DENAKVKGTGSGF+WDK
Sbjct: 61  VPQEEDRIVGLFQETSPSVVYIKNLEIAKKPQNPSEEAMLIEDDENAKVKGTGSGFIWDK 120

Query: 146 FGHIVTNYHVVSALATDNSGLQRCKVLFVNLVDVKGNGIYKEAKIVGFDPEYDLAVLKVL 205
           FGHIVTNYHVVSALATDNSGLQRCK   VNLVDVKGNGI ++AKIVGFDPEYDLAVLK  
Sbjct: 121 FGHIVTNYHVVSALATDNSGLQRCK---VNLVDVKGNGISRDAKIVGFDPEYDLAVLK-- 180

Query: 206 NQACFEWLHSVSTFMKSLLKLVELEGQELKPIVFGASRNLRVGQSCYAIGNPFGYEKTLT 265
                                VELEG ELKPIVFG SR+LRVGQSCYAIGNPFGYEKTLT
Sbjct: 181 ---------------------VELEGHELKPIVFGTSRSLRVGQSCYAIGNPFGYEKTLT 240

Query: 266 AGVISGLGREIPSPNGRAIRGAIQTDAAISA-------------------------GTGM 325
           AGVISGLGREIPSPNGRAIRGAIQTDAAISA                         GTGM
Sbjct: 241 AGVISGLGREIPSPNGRAIRGAIQTDAAISAGNSGGPLVDLYGHVIGVNTATFTRKGTGM 300

Query: 326 SSGVNFAIPIDTVVRTVPYLIVYGTPYSERF 331
           SSGVNFAIPIDTVVRTVPYLIVYGTPYSERF
Sbjct: 301 SSGVNFAIPIDTVVRTVPYLIVYGTPYSERF 305

BLAST of CaUC03G057640 vs. NCBI nr
Match: XP_008444456.1 (PREDICTED: protease Do-like 5, chloroplastic [Cucumis melo] >KAA0053993.1 protease Do-like 5 [Cucumis melo var. makuwa] >TYK20678.1 protease Do-like 5 [Cucumis melo var. makuwa])

HSP 1 Score: 467.2 bits (1201), Expect = 1.1e-127
Identity = 254/331 (76.74%), Postives = 263/331 (79.46%), Query Frame = 0

Query: 26  MALGSLGIRVLPVSASPNPSENPLPFTSRRAIVFAPTALMASLLAFPLPTYAALPQLQDD 85
           MALG LGI  LP+ A PN SENPLPFTSRRAI+F+PTALMASLLAFPLPT AALPQLQD 
Sbjct: 1   MALGLLGIPPLPIPAPPNSSENPLPFTSRRAILFSPTALMASLLAFPLPTPAALPQLQDP 60

Query: 86  IPQEEDRIINLFQETSPSVVYIKDLEIAKNPQNHSEE-AILIEDENAKVKGTGSGFVWDK 145
           + QEEDRI++LFQETSPSVVYIKDLE+AKNPQN SEE  +LIED+N KVKGTGSGFVWDK
Sbjct: 61  LLQEEDRIVSLFQETSPSVVYIKDLELAKNPQNRSEEPPMLIEDDNVKVKGTGSGFVWDK 120

Query: 146 FGHIVTNYHVVSALATDNSGLQRCKVLFVNLVDVKGNGIYKEAKIVGFDPEYDLAVLKVL 205
           FGHIVTNYHVVSALATDNSG QRCK   VNLVDVKGNGIYKEA IVGFDPEYDLAVLK  
Sbjct: 121 FGHIVTNYHVVSALATDNSGSQRCK---VNLVDVKGNGIYKEANIVGFDPEYDLAVLK-- 180

Query: 206 NQACFEWLHSVSTFMKSLLKLVELEGQELKPIVFGASRNLRVGQSCYAIGNPFGYEKTLT 265
                                VELEG ELKPIVFG SRNLRVGQSCYAIGNPFGYEKTLT
Sbjct: 181 ---------------------VELEGHELKPIVFGTSRNLRVGQSCYAIGNPFGYEKTLT 240

Query: 266 AGVISGLGREIPSPNGRAIRGAIQTDAAISA-------------------------GTGM 325
           AGVISGLGREIPSPNGRAIRGAIQTDAAISA                         GTGM
Sbjct: 241 AGVISGLGREIPSPNGRAIRGAIQTDAAISAGNSGGPLVDSYGHVIGVNTATFTRKGTGM 300

Query: 326 SSGVNFAIPIDTVVRTVPYLIVYGTPYSERF 331
           SSGVNFAIPIDTVVRTVPYLIVYGTPYSERF
Sbjct: 301 SSGVNFAIPIDTVVRTVPYLIVYGTPYSERF 305

BLAST of CaUC03G057640 vs. NCBI nr
Match: XP_023520040.1 (protease Do-like 5, chloroplastic isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 464.2 bits (1193), Expect = 9.7e-127
Identity = 251/331 (75.83%), Postives = 267/331 (80.66%), Query Frame = 0

Query: 26  MALGSLGIRVLPVSASPNPSENPLPFTSRRAIVFAPTALMASLLAFPLPTYAALPQLQDD 85
           MA GSLGIRVLPVS+ PN SE  LPFTSRRAIVFAPTALMASLLAFP+P++AALPQLQD+
Sbjct: 1   MASGSLGIRVLPVSSPPNSSEISLPFTSRRAIVFAPTALMASLLAFPVPSFAALPQLQDE 60

Query: 86  IPQEEDRIINLFQETSPSVVYIKDLEIAKNPQNHSEEAILIE-DENAKVKGTGSGFVWDK 145
           +PQEEDRI+ LFQETSPSVV IK+LEIAK PQN SEEA+LIE DENAKVKGTGSGF+WDK
Sbjct: 61  VPQEEDRIVGLFQETSPSVVCIKNLEIAKKPQNPSEEAMLIEDDENAKVKGTGSGFIWDK 120

Query: 146 FGHIVTNYHVVSALATDNSGLQRCKVLFVNLVDVKGNGIYKEAKIVGFDPEYDLAVLKVL 205
           FGHIVTNYHVVSALATDNSGLQRCK   VNL+DVKGNGI ++AK+VGFDPEYDLAVLK  
Sbjct: 121 FGHIVTNYHVVSALATDNSGLQRCK---VNLLDVKGNGISRDAKVVGFDPEYDLAVLK-- 180

Query: 206 NQACFEWLHSVSTFMKSLLKLVELEGQELKPIVFGASRNLRVGQSCYAIGNPFGYEKTLT 265
                                VEL+G ELKPIVFG SR+LRVGQSCYAIGNPFGYEKTLT
Sbjct: 181 ---------------------VELQGHELKPIVFGTSRSLRVGQSCYAIGNPFGYEKTLT 240

Query: 266 AGVISGLGREIPSPNGRAIRGAIQTDAAISA-------------------------GTGM 325
           AGVISGLGREIPSPNGRAIRGAIQTDAAISA                         GTGM
Sbjct: 241 AGVISGLGREIPSPNGRAIRGAIQTDAAISAGNSGGPLVDLYGHVIGVNTATFTRKGTGM 300

Query: 326 SSGVNFAIPIDTVVRTVPYLIVYGTPYSERF 331
           SSGVNFAIPIDTVVRTVPYLIVYGTPYSERF
Sbjct: 301 SSGVNFAIPIDTVVRTVPYLIVYGTPYSERF 305

BLAST of CaUC03G057640 vs. NCBI nr
Match: XP_023001449.1 (protease Do-like 5, chloroplastic isoform X1 [Cucurbita maxima])

HSP 1 Score: 459.9 bits (1182), Expect = 1.8e-125
Identity = 250/331 (75.53%), Postives = 266/331 (80.36%), Query Frame = 0

Query: 26  MALGSLGIRVLPVSASPNPSENPLPFTSRRAIVFAPTALMASLLAFPLPTYAALPQLQDD 85
           MALGSLGIR+LPVS+ PN SE  LPF+SRRAIVFAPT LMASLLAFP+P++AAL QLQD+
Sbjct: 1   MALGSLGIRLLPVSSPPNSSEISLPFSSRRAIVFAPTVLMASLLAFPVPSFAALLQLQDE 60

Query: 86  IPQEEDRIINLFQETSPSVVYIKDLEIAKNPQNHSEEAILIE-DENAKVKGTGSGFVWDK 145
           +PQEEDRI+ LFQETSPSVV IK+LEIAK PQN SEEA+LIE DENAKVKGTGSGF+WDK
Sbjct: 61  VPQEEDRIVGLFQETSPSVVCIKNLEIAKKPQNPSEEAMLIEDDENAKVKGTGSGFIWDK 120

Query: 146 FGHIVTNYHVVSALATDNSGLQRCKVLFVNLVDVKGNGIYKEAKIVGFDPEYDLAVLKVL 205
           FGHIVTNYHVVSALATDNSGLQRCK   VNLVDVKGNGI ++AKIVGFDPEYDLAVLK  
Sbjct: 121 FGHIVTNYHVVSALATDNSGLQRCK---VNLVDVKGNGISRDAKIVGFDPEYDLAVLK-- 180

Query: 206 NQACFEWLHSVSTFMKSLLKLVELEGQELKPIVFGASRNLRVGQSCYAIGNPFGYEKTLT 265
                                VEL+G ELKPIVFG SR+LRVGQSCYAIGNPFGYEKTLT
Sbjct: 181 ---------------------VELQGHELKPIVFGTSRSLRVGQSCYAIGNPFGYEKTLT 240

Query: 266 AGVISGLGREIPSPNGRAIRGAIQTDAAISA-------------------------GTGM 325
           AGVISGLGREIPSPNGRAIRGAIQTDAAISA                         GTGM
Sbjct: 241 AGVISGLGREIPSPNGRAIRGAIQTDAAISAGNSGGPLVDLYGHVIGVNTATFTRKGTGM 300

Query: 326 SSGVNFAIPIDTVVRTVPYLIVYGTPYSERF 331
           SSGVNFAIPIDTVVRTVPYLIVYGTPYSERF
Sbjct: 301 SSGVNFAIPIDTVVRTVPYLIVYGTPYSERF 305

BLAST of CaUC03G057640 vs. ExPASy Swiss-Prot
Match: Q9SEL7 (Protease Do-like 5, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DEGP5 PE=1 SV=3)

HSP 1 Score: 287.0 bits (733), Expect = 2.8e-76
Identity = 164/311 (52.73%), Postives = 202/311 (64.95%), Query Frame = 0

Query: 54  RRAIVFAPT-ALMASLLA-----FPLPTYAALPQL---QDDIPQEEDRIINLFQETSPSV 113
           RR ++F  + AL +SLL       P+ +  AL Q    ++++ +EE+R +NLFQ+TSPSV
Sbjct: 43  RRIMIFGSSLALTSSLLGSNQQRLPMESAIALEQFKEKEEELEEEEERNVNLFQKTSPSV 102

Query: 114 VYIKDLEIAKNPQNHSEEAILIEDENAKVKGTGSGFVWDKFGHIVTNYHVVSALATDNSG 173
           VYI+ +E+ K     S   IL ++EN K++GTGSGFVWDK GHIVTNYHV++ LATD  G
Sbjct: 103 VYIEAIELPKT----SSGDILTDEENGKIEGTGSGFVWDKLGHIVTNYHVIAKLATDQFG 162

Query: 174 LQRCKVLFVNLVDVKGNGIYKEAKIVGFDPEYDLAVLKVLNQACFEWLHSVSTFMKSLLK 233
           LQRCK   V+LVD KG    KE KIVG DP+ DLAVLK                      
Sbjct: 163 LQRCK---VSLVDAKGTRFSKEGKIVGLDPDNDLAVLK---------------------- 222

Query: 234 LVELEGQELKPIVFGASRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIR 293
            +E EG+EL P+V G S +LRVGQSC+AIGNP+GYE TLT GV+SGLGREIPSPNG++I 
Sbjct: 223 -IETEGRELNPVVLGTSNDLRVGQSCFAIGNPYGYENTLTIGVVSGLGREIPSPNGKSIS 282

Query: 294 GAIQTDAAISA-------------------------GTGMSSGVNFAIPIDTVVRTVPYL 331
            AIQTDA I++                         G+GMSSGVNFAIPIDTVVRTVPYL
Sbjct: 283 EAIQTDADINSGNSGGPLLDSYGHTIGVNTATFTRKGSGMSSGVNFAIPIDTVVRTVPYL 323

BLAST of CaUC03G057640 vs. ExPASy Swiss-Prot
Match: O22609 (Protease Do-like 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DEGP1 PE=1 SV=2)

HSP 1 Score: 141.0 bits (354), Expect = 2.5e-32
Identity = 112/312 (35.90%), Postives = 153/312 (49.04%), Query Frame = 0

Query: 46  ENPLPFTSRRAIVFAPTALMASLLAFPLPTYAALPQLQD----------DIPQEEDRIIN 105
           ++ L FT   A+   P  L+ + +A     +AA P ++            +  +E   + 
Sbjct: 66  DDTLHFTPFSAV--KPFFLLCTSVALSFSLFAASPAVESASAFVVSTPKKLQTDELATVR 125

Query: 106 LFQETSPSVVYIKDLEIAKNPQNHSEEAILIEDENAKVKGTGSGFVWDKFGHIVTNYHVV 165
           LFQE +PSVVYI +L +        ++A  + D     +G+GSGFVWDK GHIVTNYHV+
Sbjct: 126 LFQENTPSVVYITNLAV-------RQDAFTL-DVLEVPQGSGSGFVWDKQGHIVTNYHVI 185

Query: 166 SALATDNSGLQRCKVLFVNLVDVKGNGIYKEAKIVGFDPEYDLAVLKVLNQACFEWLHSV 225
              A+D         L V L D        +AK+VGFD + D+AVL+             
Sbjct: 186 RG-ASD---------LRVTLAD----QTTFDAKVVGFDQDKDVAVLR------------- 245

Query: 226 STFMKSLLKLVELEGQELKPIVFGASRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGREI 285
                     ++    +L+PI  G S +L VGQ  +AIGNPFG + TLT GVISGL REI
Sbjct: 246 ----------IDAPKNKLRPIPVGVSADLLVGQKVFAIGNPFGLDHTLTTGVISGLRREI 305

Query: 286 PS-PNGRAIRGAIQTDAAISAG-----------------------TGMSSGVNFAIPIDT 324
            S   GR I+  IQTDAAI+ G                       +G SSGV F+IP+DT
Sbjct: 306 SSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGTLIGINTAIYSPSGASSGVGFSIPVDT 330

BLAST of CaUC03G057640 vs. ExPASy Swiss-Prot
Match: Q9LU10 (Protease Do-like 8, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DEGP8 PE=1 SV=1)

HSP 1 Score: 134.4 bits (337), Expect = 2.3e-30
Identity = 112/312 (35.90%), Postives = 142/312 (45.51%), Query Frame = 0

Query: 34  RVLPVSASPNPSENPLPFTSRRAIVFAPTALMASLLAFPLPTYAALPQLQDDIPQEEDRI 93
           R+L  S   N   NP  + S  A+     A +  +     P     P         E RI
Sbjct: 68  RILLTSLFMNLCFNPSRYLSALALGDPSVATVEDVSPTVFPAGPLFP--------TEGRI 127

Query: 94  INLFQETSPSVVYIKDLEIAKNPQNHSEEAILIEDENAKVKGTGSGFVWDKFGHIVTNYH 153
           + LF++ + SVV I D+ +   PQ      + I +      G GSG VWD  G+IVTNYH
Sbjct: 128 VQLFEKNTYSVVNIFDVTL--RPQLKMTGVVEIPE------GNGSGVVWDGQGYIVTNYH 187

Query: 154 VVSALATDNSGLQRCKVLFVNLVDVKGNGIYKEAKIVGFDPEYDLAVLKVLNQACFEWLH 213
           V+    + N       V  VN++   G     E K+VG D   DLAVLK           
Sbjct: 188 VIGNALSRNPS-PGDVVGRVNILASDGVQKNFEGKLVGADRAKDLAVLK----------- 247

Query: 214 SVSTFMKSLLKLVELEGQELKPIVFGASRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGR 273
                       V+     LKPI  G S +L+VGQ C AIGNPFG++ TLT GVISGL R
Sbjct: 248 ------------VDAPETLLKPIKVGQSNSLKVGQQCLAIGNPFGFDHTLTVGVISGLNR 307

Query: 274 EIPSPNGRAIRGAIQTDAAISAG-----------------------TGMSSGVNFAIPID 323
           +I S  G  I G IQTDAAI+ G                       TG S+GV FAIP  
Sbjct: 308 DIFSQTGVTIGGGIQTDAAINPGNSGGPLLDSKGNLIGINTAIFTQTGTSAGVGFAIPSS 339

BLAST of CaUC03G057640 vs. ExPASy Swiss-Prot
Match: Q2SL36 (Probable periplasmic serine endoprotease DegP-like OS=Hahella chejuensis (strain KCTC 2396) OX=349521 GN=mucD PE=3 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 5.5e-16
Identity = 67/205 (32.68%), Postives = 94/205 (45.85%), Query Frame = 0

Query: 132 KVKGTGSGFVWDKFGHIVTNYHVVSALATDNSGLQRCKVLFVNLVDVKGNGIYKEAKIVG 191
           + + TGSGF+  K G+I+TN HVV+              +FV L+D +       AK++G
Sbjct: 87  EAQSTGSGFIVSKDGYILTNNHVVAG----------ADEIFVRLMDRR----ELTAKLIG 146

Query: 192 FDPEYDLAVLKVLNQACFEWLHSVSTFMKSLLKLVELEGQELKPIVFGASRNLRVGQSCY 251
            D + DLAVLKV                         E  +L  +  G S  L+VG+   
Sbjct: 147 SDEKSDLAVLKV-------------------------EADDLPVLNLGKSSELKVGEWVV 206

Query: 252 AIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTDAAISAG--------------- 311
           AIG+PFG+E T+TAG++S  GR +P+ N       IQTD AI+ G               
Sbjct: 207 AIGSPFGFEYTVTAGIVSAKGRSLPNENYVPF---IQTDVAINPGNSGGPLFNLEGEVVG 249

Query: 312 --------TGMSSGVNFAIPIDTVV 314
                   +G   GV+FAIPID  +
Sbjct: 267 INSQIYTRSGGFMGVSFAIPIDVAL 249

BLAST of CaUC03G057640 vs. ExPASy Swiss-Prot
Match: Q4KGQ4 (Probable periplasmic serine endoprotease DegP-like OS=Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5) OX=220664 GN=mucD PE=3 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 6.7e-14
Identity = 59/209 (28.23%), Postives = 93/209 (44.50%), Query Frame = 0

Query: 128 DENAKVKGTGSGFVWDKFGHIVTNYHVVSALATDNSGLQRCKVLFVNLVDVKGNGIYKEA 187
           D   + +  GSGF+    G+I+TN HV++              + V L D        +A
Sbjct: 91  DRQREAQSLGSGFIISADGYILTNNHVIA----------DADEILVRLADRS----ELKA 150

Query: 188 KIVGFDPEYDLAVLKVLNQACFEWLHSVSTFMKSLLKLVELEGQELKPIVFGASRNLRVG 247
           K++G DP  D+A+LK+                         +G++L  +  G S++L+ G
Sbjct: 151 KLIGTDPRSDVALLKI-------------------------DGKDLPVLKLGKSQDLKAG 210

Query: 248 QSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIRGAIQTDAAISAG----------- 307
           Q   AIG+PFG++ T+T G++S +GR +P+ N       IQTD  I+ G           
Sbjct: 211 QWVVAIGSPFGFDHTVTQGIVSAIGRSLPNENYVPF---IQTDVPINPGNSGGPLFNLAG 257

Query: 308 ------------TGMSSGVNFAIPIDTVV 314
                       +G   GV+FAIPID  +
Sbjct: 271 EVVGINSQIYTRSGGFMGVSFAIPIDVAM 257

BLAST of CaUC03G057640 vs. ExPASy TrEMBL
Match: A0A6J1EKF8 (protease Do-like 5, chloroplastic isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111434145 PE=3 SV=1)

HSP 1 Score: 471.5 bits (1212), Expect = 2.9e-129
Identity = 255/331 (77.04%), Postives = 269/331 (81.27%), Query Frame = 0

Query: 26  MALGSLGIRVLPVSASPNPSENPLPFTSRRAIVFAPTALMASLLAFPLPTYAALPQLQDD 85
           MALGSLGIR+LPVS+ PN SE  LPFTSRRAIVFAPTALMASLLAFP+P++AALPQLQD+
Sbjct: 1   MALGSLGIRLLPVSSPPNSSEISLPFTSRRAIVFAPTALMASLLAFPVPSFAALPQLQDE 60

Query: 86  IPQEEDRIINLFQETSPSVVYIKDLEIAKNPQNHSEEAILIE-DENAKVKGTGSGFVWDK 145
           +PQEEDRI+ LFQETSPSVVYIK+LEIAK PQN SEEA+LIE DENAKVKGTGSGF+WDK
Sbjct: 61  VPQEEDRIVGLFQETSPSVVYIKNLEIAKKPQNPSEEAMLIEDDENAKVKGTGSGFIWDK 120

Query: 146 FGHIVTNYHVVSALATDNSGLQRCKVLFVNLVDVKGNGIYKEAKIVGFDPEYDLAVLKVL 205
           FGHIVTNYHVVSALATDNSGLQRCK   VNLVDVKGNGI ++AKIVGFDPEYDLAVLK  
Sbjct: 121 FGHIVTNYHVVSALATDNSGLQRCK---VNLVDVKGNGISRDAKIVGFDPEYDLAVLK-- 180

Query: 206 NQACFEWLHSVSTFMKSLLKLVELEGQELKPIVFGASRNLRVGQSCYAIGNPFGYEKTLT 265
                                VELEG ELKPIVFG SR+LRVGQSCYAIGNPFGYEKTLT
Sbjct: 181 ---------------------VELEGHELKPIVFGTSRSLRVGQSCYAIGNPFGYEKTLT 240

Query: 266 AGVISGLGREIPSPNGRAIRGAIQTDAAISA-------------------------GTGM 325
           AGVISGLGREIPSPNGRAIRGAIQTDAAISA                         GTGM
Sbjct: 241 AGVISGLGREIPSPNGRAIRGAIQTDAAISAGNSGGPLVDLYGHVIGVNTATFTRKGTGM 300

Query: 326 SSGVNFAIPIDTVVRTVPYLIVYGTPYSERF 331
           SSGVNFAIPIDTVVRTVPYLIVYGTPYSERF
Sbjct: 301 SSGVNFAIPIDTVVRTVPYLIVYGTPYSERF 305

BLAST of CaUC03G057640 vs. ExPASy TrEMBL
Match: A0A5D3DAW0 (Protease Do-like 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold480G00660 PE=3 SV=1)

HSP 1 Score: 467.2 bits (1201), Expect = 5.5e-128
Identity = 254/331 (76.74%), Postives = 263/331 (79.46%), Query Frame = 0

Query: 26  MALGSLGIRVLPVSASPNPSENPLPFTSRRAIVFAPTALMASLLAFPLPTYAALPQLQDD 85
           MALG LGI  LP+ A PN SENPLPFTSRRAI+F+PTALMASLLAFPLPT AALPQLQD 
Sbjct: 1   MALGLLGIPPLPIPAPPNSSENPLPFTSRRAILFSPTALMASLLAFPLPTPAALPQLQDP 60

Query: 86  IPQEEDRIINLFQETSPSVVYIKDLEIAKNPQNHSEE-AILIEDENAKVKGTGSGFVWDK 145
           + QEEDRI++LFQETSPSVVYIKDLE+AKNPQN SEE  +LIED+N KVKGTGSGFVWDK
Sbjct: 61  LLQEEDRIVSLFQETSPSVVYIKDLELAKNPQNRSEEPPMLIEDDNVKVKGTGSGFVWDK 120

Query: 146 FGHIVTNYHVVSALATDNSGLQRCKVLFVNLVDVKGNGIYKEAKIVGFDPEYDLAVLKVL 205
           FGHIVTNYHVVSALATDNSG QRCK   VNLVDVKGNGIYKEA IVGFDPEYDLAVLK  
Sbjct: 121 FGHIVTNYHVVSALATDNSGSQRCK---VNLVDVKGNGIYKEANIVGFDPEYDLAVLK-- 180

Query: 206 NQACFEWLHSVSTFMKSLLKLVELEGQELKPIVFGASRNLRVGQSCYAIGNPFGYEKTLT 265
                                VELEG ELKPIVFG SRNLRVGQSCYAIGNPFGYEKTLT
Sbjct: 181 ---------------------VELEGHELKPIVFGTSRNLRVGQSCYAIGNPFGYEKTLT 240

Query: 266 AGVISGLGREIPSPNGRAIRGAIQTDAAISA-------------------------GTGM 325
           AGVISGLGREIPSPNGRAIRGAIQTDAAISA                         GTGM
Sbjct: 241 AGVISGLGREIPSPNGRAIRGAIQTDAAISAGNSGGPLVDSYGHVIGVNTATFTRKGTGM 300

Query: 326 SSGVNFAIPIDTVVRTVPYLIVYGTPYSERF 331
           SSGVNFAIPIDTVVRTVPYLIVYGTPYSERF
Sbjct: 301 SSGVNFAIPIDTVVRTVPYLIVYGTPYSERF 305

BLAST of CaUC03G057640 vs. ExPASy TrEMBL
Match: A0A1S3B9W5 (protease Do-like 5, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103487774 PE=3 SV=1)

HSP 1 Score: 467.2 bits (1201), Expect = 5.5e-128
Identity = 254/331 (76.74%), Postives = 263/331 (79.46%), Query Frame = 0

Query: 26  MALGSLGIRVLPVSASPNPSENPLPFTSRRAIVFAPTALMASLLAFPLPTYAALPQLQDD 85
           MALG LGI  LP+ A PN SENPLPFTSRRAI+F+PTALMASLLAFPLPT AALPQLQD 
Sbjct: 1   MALGLLGIPPLPIPAPPNSSENPLPFTSRRAILFSPTALMASLLAFPLPTPAALPQLQDP 60

Query: 86  IPQEEDRIINLFQETSPSVVYIKDLEIAKNPQNHSEE-AILIEDENAKVKGTGSGFVWDK 145
           + QEEDRI++LFQETSPSVVYIKDLE+AKNPQN SEE  +LIED+N KVKGTGSGFVWDK
Sbjct: 61  LLQEEDRIVSLFQETSPSVVYIKDLELAKNPQNRSEEPPMLIEDDNVKVKGTGSGFVWDK 120

Query: 146 FGHIVTNYHVVSALATDNSGLQRCKVLFVNLVDVKGNGIYKEAKIVGFDPEYDLAVLKVL 205
           FGHIVTNYHVVSALATDNSG QRCK   VNLVDVKGNGIYKEA IVGFDPEYDLAVLK  
Sbjct: 121 FGHIVTNYHVVSALATDNSGSQRCK---VNLVDVKGNGIYKEANIVGFDPEYDLAVLK-- 180

Query: 206 NQACFEWLHSVSTFMKSLLKLVELEGQELKPIVFGASRNLRVGQSCYAIGNPFGYEKTLT 265
                                VELEG ELKPIVFG SRNLRVGQSCYAIGNPFGYEKTLT
Sbjct: 181 ---------------------VELEGHELKPIVFGTSRNLRVGQSCYAIGNPFGYEKTLT 240

Query: 266 AGVISGLGREIPSPNGRAIRGAIQTDAAISA-------------------------GTGM 325
           AGVISGLGREIPSPNGRAIRGAIQTDAAISA                         GTGM
Sbjct: 241 AGVISGLGREIPSPNGRAIRGAIQTDAAISAGNSGGPLVDSYGHVIGVNTATFTRKGTGM 300

Query: 326 SSGVNFAIPIDTVVRTVPYLIVYGTPYSERF 331
           SSGVNFAIPIDTVVRTVPYLIVYGTPYSERF
Sbjct: 301 SSGVNFAIPIDTVVRTVPYLIVYGTPYSERF 305

BLAST of CaUC03G057640 vs. ExPASy TrEMBL
Match: A0A6J1KMS0 (protease Do-like 5, chloroplastic isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111495580 PE=3 SV=1)

HSP 1 Score: 459.9 bits (1182), Expect = 8.9e-126
Identity = 250/331 (75.53%), Postives = 266/331 (80.36%), Query Frame = 0

Query: 26  MALGSLGIRVLPVSASPNPSENPLPFTSRRAIVFAPTALMASLLAFPLPTYAALPQLQDD 85
           MALGSLGIR+LPVS+ PN SE  LPF+SRRAIVFAPT LMASLLAFP+P++AAL QLQD+
Sbjct: 1   MALGSLGIRLLPVSSPPNSSEISLPFSSRRAIVFAPTVLMASLLAFPVPSFAALLQLQDE 60

Query: 86  IPQEEDRIINLFQETSPSVVYIKDLEIAKNPQNHSEEAILIE-DENAKVKGTGSGFVWDK 145
           +PQEEDRI+ LFQETSPSVV IK+LEIAK PQN SEEA+LIE DENAKVKGTGSGF+WDK
Sbjct: 61  VPQEEDRIVGLFQETSPSVVCIKNLEIAKKPQNPSEEAMLIEDDENAKVKGTGSGFIWDK 120

Query: 146 FGHIVTNYHVVSALATDNSGLQRCKVLFVNLVDVKGNGIYKEAKIVGFDPEYDLAVLKVL 205
           FGHIVTNYHVVSALATDNSGLQRCK   VNLVDVKGNGI ++AKIVGFDPEYDLAVLK  
Sbjct: 121 FGHIVTNYHVVSALATDNSGLQRCK---VNLVDVKGNGISRDAKIVGFDPEYDLAVLK-- 180

Query: 206 NQACFEWLHSVSTFMKSLLKLVELEGQELKPIVFGASRNLRVGQSCYAIGNPFGYEKTLT 265
                                VEL+G ELKPIVFG SR+LRVGQSCYAIGNPFGYEKTLT
Sbjct: 181 ---------------------VELQGHELKPIVFGTSRSLRVGQSCYAIGNPFGYEKTLT 240

Query: 266 AGVISGLGREIPSPNGRAIRGAIQTDAAISA-------------------------GTGM 325
           AGVISGLGREIPSPNGRAIRGAIQTDAAISA                         GTGM
Sbjct: 241 AGVISGLGREIPSPNGRAIRGAIQTDAAISAGNSGGPLVDLYGHVIGVNTATFTRKGTGM 300

Query: 326 SSGVNFAIPIDTVVRTVPYLIVYGTPYSERF 331
           SSGVNFAIPIDTVVRTVPYLIVYGTPYSERF
Sbjct: 301 SSGVNFAIPIDTVVRTVPYLIVYGTPYSERF 305

BLAST of CaUC03G057640 vs. ExPASy TrEMBL
Match: A0A6J1CEE6 (protease Do-like 5, chloroplastic isoform X2 OS=Momordica charantia OX=3673 GN=LOC111010776 PE=3 SV=1)

HSP 1 Score: 459.9 bits (1182), Expect = 8.9e-126
Identity = 244/330 (73.94%), Postives = 259/330 (78.48%), Query Frame = 0

Query: 26  MALGSLGIRVLPVSASPNPSENPLPFTSRRAIVFAPTALMASLLAFPLPTYAALPQLQDD 85
           MAL SLGI +LP+ A PN S N LPFTSRRA+VFAP+ALMASLLAFPLPT+AALPQ+Q  
Sbjct: 1   MALASLGIHLLPIPAPPNSSHNSLPFTSRRALVFAPSALMASLLAFPLPTHAALPQIQAQ 60

Query: 86  IPQEEDRIINLFQETSPSVVYIKDLEIAKNPQNHSEEAILIEDENAKVKGTGSGFVWDKF 145
           +PQEEDR++ LFQ+ SPSVVYIKDLE+AK PQN SEEA+L+EDEN KVKGTGSGFVWDKF
Sbjct: 61  VPQEEDRVVALFQDASPSVVYIKDLELAKKPQNSSEEALLVEDENVKVKGTGSGFVWDKF 120

Query: 146 GHIVTNYHVVSALATDNSGLQRCKVLFVNLVDVKGNGIYKEAKIVGFDPEYDLAVLKVLN 205
           GHIVTNYHVVSALATDNSGLQRCK   VNLVD KGNGIY+EAKIVGFDPEYDLAVLK   
Sbjct: 121 GHIVTNYHVVSALATDNSGLQRCK---VNLVDAKGNGIYREAKIVGFDPEYDLAVLK--- 180

Query: 206 QACFEWLHSVSTFMKSLLKLVELEGQELKPIVFGASRNLRVGQSCYAIGNPFGYEKTLTA 265
                               VEL G ELKPIV G SRNLRVGQSCYAIGNPFGYEKTLTA
Sbjct: 181 --------------------VELGGCELKPIVLGTSRNLRVGQSCYAIGNPFGYEKTLTA 240

Query: 266 GVISGLGREIPSPNGRAIRGAIQTDAAISA-------------------------GTGMS 325
           GVISGLGREIPSPNGRAIRG IQTDAAIS+                         GTGMS
Sbjct: 241 GVISGLGREIPSPNGRAIRGGIQTDAAISSGNSGGPLIDSYGHVIGVNTATFTRKGTGMS 300

Query: 326 SGVNFAIPIDTVVRTVPYLIVYGTPYSERF 331
           SGVNFAIPIDTVVRTVPYLIVYGTPYSERF
Sbjct: 301 SGVNFAIPIDTVVRTVPYLIVYGTPYSERF 304

BLAST of CaUC03G057640 vs. TAIR 10
Match: AT4G18370.1 (DEGP protease 5 )

HSP 1 Score: 287.0 bits (733), Expect = 2.0e-77
Identity = 164/311 (52.73%), Postives = 202/311 (64.95%), Query Frame = 0

Query: 54  RRAIVFAPT-ALMASLLA-----FPLPTYAALPQL---QDDIPQEEDRIINLFQETSPSV 113
           RR ++F  + AL +SLL       P+ +  AL Q    ++++ +EE+R +NLFQ+TSPSV
Sbjct: 43  RRIMIFGSSLALTSSLLGSNQQRLPMESAIALEQFKEKEEELEEEEERNVNLFQKTSPSV 102

Query: 114 VYIKDLEIAKNPQNHSEEAILIEDENAKVKGTGSGFVWDKFGHIVTNYHVVSALATDNSG 173
           VYI+ +E+ K     S   IL ++EN K++GTGSGFVWDK GHIVTNYHV++ LATD  G
Sbjct: 103 VYIEAIELPKT----SSGDILTDEENGKIEGTGSGFVWDKLGHIVTNYHVIAKLATDQFG 162

Query: 174 LQRCKVLFVNLVDVKGNGIYKEAKIVGFDPEYDLAVLKVLNQACFEWLHSVSTFMKSLLK 233
           LQRCK   V+LVD KG    KE KIVG DP+ DLAVLK                      
Sbjct: 163 LQRCK---VSLVDAKGTRFSKEGKIVGLDPDNDLAVLK---------------------- 222

Query: 234 LVELEGQELKPIVFGASRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGREIPSPNGRAIR 293
            +E EG+EL P+V G S +LRVGQSC+AIGNP+GYE TLT GV+SGLGREIPSPNG++I 
Sbjct: 223 -IETEGRELNPVVLGTSNDLRVGQSCFAIGNPYGYENTLTIGVVSGLGREIPSPNGKSIS 282

Query: 294 GAIQTDAAISA-------------------------GTGMSSGVNFAIPIDTVVRTVPYL 331
            AIQTDA I++                         G+GMSSGVNFAIPIDTVVRTVPYL
Sbjct: 283 EAIQTDADINSGNSGGPLLDSYGHTIGVNTATFTRKGSGMSSGVNFAIPIDTVVRTVPYL 323

BLAST of CaUC03G057640 vs. TAIR 10
Match: AT3G27925.1 (DegP protease 1 )

HSP 1 Score: 141.0 bits (354), Expect = 1.8e-33
Identity = 112/312 (35.90%), Postives = 153/312 (49.04%), Query Frame = 0

Query: 46  ENPLPFTSRRAIVFAPTALMASLLAFPLPTYAALPQLQD----------DIPQEEDRIIN 105
           ++ L FT   A+   P  L+ + +A     +AA P ++            +  +E   + 
Sbjct: 66  DDTLHFTPFSAV--KPFFLLCTSVALSFSLFAASPAVESASAFVVSTPKKLQTDELATVR 125

Query: 106 LFQETSPSVVYIKDLEIAKNPQNHSEEAILIEDENAKVKGTGSGFVWDKFGHIVTNYHVV 165
           LFQE +PSVVYI +L +        ++A  + D     +G+GSGFVWDK GHIVTNYHV+
Sbjct: 126 LFQENTPSVVYITNLAV-------RQDAFTL-DVLEVPQGSGSGFVWDKQGHIVTNYHVI 185

Query: 166 SALATDNSGLQRCKVLFVNLVDVKGNGIYKEAKIVGFDPEYDLAVLKVLNQACFEWLHSV 225
              A+D         L V L D        +AK+VGFD + D+AVL+             
Sbjct: 186 RG-ASD---------LRVTLAD----QTTFDAKVVGFDQDKDVAVLR------------- 245

Query: 226 STFMKSLLKLVELEGQELKPIVFGASRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGREI 285
                     ++    +L+PI  G S +L VGQ  +AIGNPFG + TLT GVISGL REI
Sbjct: 246 ----------IDAPKNKLRPIPVGVSADLLVGQKVFAIGNPFGLDHTLTTGVISGLRREI 305

Query: 286 PS-PNGRAIRGAIQTDAAISAG-----------------------TGMSSGVNFAIPIDT 324
            S   GR I+  IQTDAAI+ G                       +G SSGV F+IP+DT
Sbjct: 306 SSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGTLIGINTAIYSPSGASSGVGFSIPVDT 330

BLAST of CaUC03G057640 vs. TAIR 10
Match: AT5G39830.1 (Trypsin family protein with PDZ domain )

HSP 1 Score: 134.4 bits (337), Expect = 1.6e-31
Identity = 112/312 (35.90%), Postives = 142/312 (45.51%), Query Frame = 0

Query: 34  RVLPVSASPNPSENPLPFTSRRAIVFAPTALMASLLAFPLPTYAALPQLQDDIPQEEDRI 93
           R+L  S   N   NP  + S  A+     A +  +     P     P         E RI
Sbjct: 68  RILLTSLFMNLCFNPSRYLSALALGDPSVATVEDVSPTVFPAGPLFP--------TEGRI 127

Query: 94  INLFQETSPSVVYIKDLEIAKNPQNHSEEAILIEDENAKVKGTGSGFVWDKFGHIVTNYH 153
           + LF++ + SVV I D+ +   PQ      + I +      G GSG VWD  G+IVTNYH
Sbjct: 128 VQLFEKNTYSVVNIFDVTL--RPQLKMTGVVEIPE------GNGSGVVWDGQGYIVTNYH 187

Query: 154 VVSALATDNSGLQRCKVLFVNLVDVKGNGIYKEAKIVGFDPEYDLAVLKVLNQACFEWLH 213
           V+    + N       V  VN++   G     E K+VG D   DLAVLK           
Sbjct: 188 VIGNALSRNPS-PGDVVGRVNILASDGVQKNFEGKLVGADRAKDLAVLK----------- 247

Query: 214 SVSTFMKSLLKLVELEGQELKPIVFGASRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGR 273
                       V+     LKPI  G S +L+VGQ C AIGNPFG++ TLT GVISGL R
Sbjct: 248 ------------VDAPETLLKPIKVGQSNSLKVGQQCLAIGNPFGFDHTLTVGVISGLNR 307

Query: 274 EIPSPNGRAIRGAIQTDAAISAG-----------------------TGMSSGVNFAIPID 323
           +I S  G  I G IQTDAAI+ G                       TG S+GV FAIP  
Sbjct: 308 DIFSQTGVTIGGGIQTDAAINPGNSGGPLLDSKGNLIGINTAIFTQTGTSAGVGFAIPSS 339

BLAST of CaUC03G057640 vs. TAIR 10
Match: AT5G39830.2 (Trypsin family protein with PDZ domain )

HSP 1 Score: 129.0 bits (323), Expect = 6.9e-30
Identity = 108/298 (36.24%), Postives = 138/298 (46.31%), Query Frame = 0

Query: 34  RVLPVSASPNPSENPLPFTSRRAIVFAPTALMASLLAFPLPTYAALPQLQDDIPQEEDRI 93
           R+L  S   N   NP  + S  A+     A +  +     P     P         E RI
Sbjct: 68  RILLTSLFMNLCFNPSRYLSALALGDPSVATVEDVSPTVFPAGPLFP--------TEGRI 127

Query: 94  INLFQETSPSVVYIKDLEIAKNPQNHSEEAILIEDENAKVKGTGSGFVWDKFGHIVTNYH 153
           + LF++ + SVV I D+ +   PQ      + I +      G GSG VWD  G+IVTNYH
Sbjct: 128 VQLFEKNTYSVVNIFDVTL--RPQLKMTGVVEIPE------GNGSGVVWDGQGYIVTNYH 187

Query: 154 VVSALATDNSGLQRCKVLFVNLVDVKGNGIYKEAKIVGFDPEYDLAVLKVLNQACFEWLH 213
           V+    + N       V  VN++   G     E K+VG D   DLAVLK           
Sbjct: 188 VIGNALSRNPS-PGDVVGRVNILASDGVQKNFEGKLVGADRAKDLAVLK----------- 247

Query: 214 SVSTFMKSLLKLVELEGQELKPIVFGASRNLRVGQSCYAIGNPFGYEKTLTAGVISGLGR 273
                       V+     LKPI  G S +L+VGQ C AIGNPFG++ TLT GVISGL R
Sbjct: 248 ------------VDAPETLLKPIKVGQSNSLKVGQQCLAIGNPFGFDHTLTVGVISGLNR 307

Query: 274 EIPSPNGRAIRGAIQTDAAISAGT---------GMSSGVNFAIPIDTVVRTVPYLIVY 323
           +I S  G  I G IQTDAAI+ G          G   G+N AI   TV++ VP LI +
Sbjct: 308 DIFSQTGVTIGGGIQTDAAINPGNSGGPLLDSKGNLIGINTAIFTQTVLKIVPQLIQF 325

BLAST of CaUC03G057640 vs. TAIR 10
Match: AT5G27660.1 (Trypsin family protein with PDZ domain )

HSP 1 Score: 46.6 bits (109), Expect = 4.5e-05
Identity = 51/207 (24.64%), Postives = 81/207 (39.13%), Query Frame = 0

Query: 134 KGTGSGFVWDKFGHIVTNYHVVSALATDNSGLQRCKVLFVNLVDVK-GNGIYKEAKIVGF 193
           K  GSG + D  G I+T  HVV          Q  +      VDV   +G   E  +V  
Sbjct: 146 KSIGSGTIIDADGTILTCAHVV-------VDFQNIRHSSKGRVDVTLQDGRTFEGVVVNA 205

Query: 194 DPEYDLAVLKVLNQACFEWLHSVSTFMKSLLKLVELEGQELKPIVFGASRNLRVGQSCYA 253
           D + D+A++K+ ++                          L     G S  LR G    A
Sbjct: 206 DLQSDIALVKIKSKT------------------------PLPTAKLGFSSKLRPGDWVIA 265

Query: 254 IGNPFGYEKTLTAGVISGLGREIPSPN-GRAIRGAIQTDAAISAGTG------------- 313
           +G P   + T+TAG++S + R+      G   R  +QTD +I+AG               
Sbjct: 266 VGCPLSLQNTVTAGIVSCVDRKSSDLGLGGKHREYLQTDCSINAGNSGGPLVNLDGEVIG 321

Query: 314 -------MSSGVNFAIPIDTVVRTVPY 319
                   + G+ F++PID+V + + +
Sbjct: 326 VNIMKVLAADGLGFSVPIDSVSKIIEH 321

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038893976.16.5e-13178.18protease Do-like 5, chloroplastic [Benincasa hispida][more]
XP_022927238.16.1e-12977.04protease Do-like 5, chloroplastic isoform X1 [Cucurbita moschata][more]
XP_008444456.11.1e-12776.74PREDICTED: protease Do-like 5, chloroplastic [Cucumis melo] >KAA0053993.1 protea... [more]
XP_023520040.19.7e-12775.83protease Do-like 5, chloroplastic isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_023001449.11.8e-12575.53protease Do-like 5, chloroplastic isoform X1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9SEL72.8e-7652.73Protease Do-like 5, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DEGP5 PE=1 ... [more]
O226092.5e-3235.90Protease Do-like 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DEGP1 PE=1 ... [more]
Q9LU102.3e-3035.90Protease Do-like 8, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DEGP8 PE=1 ... [more]
Q2SL365.5e-1632.68Probable periplasmic serine endoprotease DegP-like OS=Hahella chejuensis (strain... [more]
Q4KGQ46.7e-1428.23Probable periplasmic serine endoprotease DegP-like OS=Pseudomonas fluorescens (s... [more]
Match NameE-valueIdentityDescription
A0A6J1EKF82.9e-12977.04protease Do-like 5, chloroplastic isoform X1 OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A5D3DAW05.5e-12876.74Protease Do-like 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold480G0... [more]
A0A1S3B9W55.5e-12876.74protease Do-like 5, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103487774 PE=3 S... [more]
A0A6J1KMS08.9e-12675.53protease Do-like 5, chloroplastic isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1CEE68.9e-12673.94protease Do-like 5, chloroplastic isoform X2 OS=Momordica charantia OX=3673 GN=L... [more]
Match NameE-valueIdentityDescription
AT4G18370.12.0e-7752.73DEGP protease 5 [more]
AT3G27925.11.8e-3335.90DegP protease 1 [more]
AT5G39830.11.6e-3135.90Trypsin family protein with PDZ domain [more]
AT5G39830.26.9e-3036.24Trypsin family protein with PDZ domain [more]
AT5G27660.14.5e-0524.64Trypsin family protein with PDZ domain [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (USVL246-FR2) v1
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001940Peptidase S1CPRINTSPR00834PROTEASES2Ccoord: 283..300
score: 42.01
coord: 246..270
score: 48.88
coord: 146..158
score: 50.47
IPR043504Peptidase S1, PA clan, chymotrypsin-like foldGENE3D2.40.10.10coord: 237..298
e-value: 1.3E-16
score: 62.8
IPR043504Peptidase S1, PA clan, chymotrypsin-like foldGENE3D2.40.10.10coord: 84..208
e-value: 2.3E-27
score: 97.0
NoneNo IPR availablePFAMPF13365Trypsin_2coord: 137..297
e-value: 1.6E-21
score: 77.8
NoneNo IPR availablePANTHERPTHR43343:SF6PROTEASE DO-LIKE 5, CHLOROPLASTIC ISOFORM X1coord: 296..330
coord: 223..296
coord: 39..203
NoneNo IPR availablePANTHERPTHR43343PEPTIDASE S12coord: 296..330
coord: 223..296
coord: 39..203
IPR009003Peptidase S1, PA clanSUPERFAMILY50494Trypsin-like serine proteasescoord: 94..316

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CaUC03G057640.1CaUC03G057640.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0043231 intracellular membrane-bounded organelle
molecular_function GO:0004252 serine-type endopeptidase activity