CaUC03G055000 (gene) Watermelon (USVL246-FR2) v1

Overview
NameCaUC03G055000
Typegene
OrganismCitrullus amarus (Watermelon (USVL246-FR2) v1)
Descriptiongibberellin 2-beta-dioxygenase 8
LocationCiama_Chr03: 5527404 .. 5533722 (+)
RNA-Seq ExpressionCaUC03G055000
SyntenyCaUC03G055000
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGTGCTTACAAAATTTAGATATTGGGGAGTGGGTTTACGTTGAAGGTTCTTTGTCCATTCATTTCCCATTCCTTTCTTATCTTCCCCAACCATTCTCATAACTCTCAATTTCCCTATATATATAACTAAATATCCTCCCCACCTCATTCTCTCACACTCTTCTCCAATTATATATTCTCTATTTCCTTCCCCTTTCTCTTTTCATTTCCCTCTCATCGAAAGGGACATTTCCCCTTTTCCCAGGTAAATAACAACAATACAAATAATAATTTCCAGTCTCATATAACATATATACACATCACTACCTAACCTCTCTCTTTTCTCTTCCCTTCTTTTTTCAGACCATTTCCAGAACCAAATATGATGCACTCTCTAAACCAGGTAAATTTAATTCAACTATACTCAAGTTAAACTTTATATAAATAGTTTGAATTGGGCGTGTTGTAGAGTGGCATGCAGTAGCTGGATTTAGCTTCTCTCTAACTTTTATTATTATTATTATTATTTTTTTTTCCCCAACTACTTTTGTTTATTCGTTTTGTTAAGAGCTGTGTTATGTCTGTACACAAGAATGGGCACACATTGAACCATATGTTTGGCCATTTTAGATCTTTCCCGATTTATAATATATTTATATAAATAGCTTCATAATTTAACTGTAATTGTCGCTCTTTGTTTTTTCAGAAAAGTTCCAAGTATCTAAATAATGGTATTATATATATATATATAAAAATAAAATAAAAAGAATAAAAATAAATTCTCTTTTTTTCTTTTTGTAATAATTGACTCGCTTAATTATTGCACTTAAAATTGGTAAATTTTACATTTACAAAAACAAATAAAATTACGGAGTTCCAACGTTGTTAAATCGAATCCTTGGTTCCTAAGATGGACACGAAGCTGGATGTCATTTTTTTTTTTTTTTAATCTTTATTTGCTGTATTTTCCATATTACAGCCGTGTCCCATCACACACGAGGGTGAACATTTTTATGAAAACGAGTCTTTGTACGGTTGGACTTACAAGAATAATGCTTCATTTATTATTTATTTATTATTATTATTTCTTTTAATTTTAAGCAAGTTATTACTACTACGTCATTGTTATTAACATTATGATTGCTAACTAATTATAAATGCTGATTTTACGTACCAAATACATAGAAACAAACCATTTTAAAGAAAATTATTTTCTACCTTTTTATTGGATCATCGTTGATATATATGTATATGTGTATATGCCGTAACAACATATCTTTAATAGTATTCAAAACTTAATTAAAATAATTTATTTTTAAGAAAATACTAAATTAATTCAAATAATACGCATAATACTTAAGGTAAATTATTTACCTTTATAATATTAACCTTAATTATTATTATTATTAATATTATTAGCTCGGTGCATGTCAATTATCATTGAAATAAAATTCACTTTTATCCATGTTTTATTATTTTAGTTCTTATCCCTTGAGAGGGGATAGAAACAGGCATCCCTTCCAAAGAAGAAAAAAAAATCAAATGCTTTATAAATATGTTAAGATTAAGTGCATGTTTGAGAATGATTATAAAATTGTTAATACTAATTTTTTTATGTTTAAAATTATTTCAAAACACGACTTTAATTAATCAAAATTAATTTAATGTTTAATTTTAAAATTTTTAATGGAATTCTTTATAGCATTGCAATTGCTTTTGAATAATTAAAAACGTGTAGTTTTGAAATTTATTTTAACTAGATTCATATATGTCCTGAGATTAAGATTATACGTACATATATAAATATTTTGCATACGATATTCACTTAATAAAATCCCTTAAGCCTACGCCTAGTCATCTCAAGAAATAAAATTTAATAAAATCTTGATTTTTTTTTTTTGAAGAAATAAAATACTGAATTATTGGTCAAGGGACATAATAATAATAATTATTATTATTTTCATTGTGTTGTAAATCATCGTTGTGGCCACAAAATAATTGGTTTATCATTTAGTGGGTCTCTCGTTTTGAAAAGTATATGCGTATGAAGAGACATGGACAATCTCAAGCTTCGTGAAATATTTTTTCTCAACAAAAAAAGGAAAAAAAAAAAAACCTTTGTAATTATAGTTCCACTTTTTCTTACAAAACCTAAAAGTGACAAACTGAAAAAATTATATATGTGTTTATATATTAAAAACAACGAAAAGAATATATGGCCCTTTGGCAAATAGAAAATACAAATTGAAAAAAAATGTTAAATTTCAAATTTGTTCTAATTTGAAAAGGTTAGTATTTAGTCCCTATAGTTTTCTAAGTTAGAATTTCATCCCTATGATTTGGAGAAAATTAGAATTTTGTTCTTATGATTTAGAAAAGTTGGAATTTAGTTTCTCCTTTAAGTCTTCTACGAGAATTTTATCAAACTATATAGACTAAATTCTAATTTTAAATCATAGAGATTAACTTTTTTCAATTCGTAGAGATTGGATTTGCAATTTAACCAATTTTATAAATTCCAGCCTAGGTAGATAGTAATTTTTTTTTTTTTTTCCGTAAACTGCTTTAGGTAGATATTAAACTTATAATGCTATATAAATTCTAGTTTTTTCTTCTTTTCCCTTTTTAAACTAGTTTAATGTAAAGTCTAGATTATTATTATATGTCTAAGGAATATATAAATTATATTGCTAAACGATATAAATATTTATTTTCTAATTTTTTAAATCAAAATATCCCTTTCATATTGAAAGTTTCCTCTTTAGTTATTATGTTGAAATCGGTAGTTTCCTTGACAATTTTAATTAAATTTAGTATCAAAATCATTTCTAAATCCTTAAAAAGTGCGAGGTTTAAATTTCTATGATGTAATATTTTCTTAATAAGTAAAAAAAAAAAAAAGAGAAAAAATATTGGTCTGAAAATTTTATTTTATATTACATAAAAAGAAACACGCATAGTAGATTGTATATTTTTTTGAGTAGGTTACATTATAAAAATATTAAAGATTATTGTTTTGATTGATGTATACCTTCATATTATATTTATCCCAGCATTAGTTTGATCAACATGTCAAAGAAATTAAAAAGAGGGGTCAATTAGAAATGGTCAAAGTATTGGGAAACATAATAGAGATGAAATAGAATTCAAATTTAGAATGAAAACAACAATCACTTCAACCAATACACACTTGTGATTTAATTTCATATATTTGAAGATGCAAACAAAAATTACACATAAAACATGTCACAGTTTTGTCACAAACAATAGAATAGATCTACACTTTTTGGGGTATGAATTGGCAAAAGAAAAAGAGAAAAAAAGAAAACCATTTGAATGAGGCAAAGGCACATGTAATATTCAGAGATGGCCTCTTTATTACATGGCAAACTGACATGCACTGTACTTCTTCTCGTTGCTTCCAGCACCTGATCCCCTAAATTAACAAACCAGATTCAAATATTTGACTTTTTTTCCGCTCCAAATAATACAAATATTTATTTATGTGTAATTGTATTCAAATATTATAACTCACCATTATAGCAAAATCAAAATGTTATTATCTATAAGTGAGATATGATAGTAGTCACATTTTATCATATTTATAAATATTTTAGTTCATTTTATTTACGTATCTCTATAGGAGCTAATATTAAATGATATGGGGTATTAACTTTAATAAAAAAATTCTACAATTCCTTTGAAAAATATGGTTTTTAGATGGTTCTTAGTCCTCTTCTTTGACCATGGCCGGTTCATTTTAGTCTTTAAAATATTTATTATATATTTCACCACTTTTAATTTTGGTAAATTTCAATGAAAATCACTTGAGTTTAATGCTATGTTCTACAGGCTAGTCAATGGTCCAAAACGTAATTTTCCACACACCAAACACCTTTTTTCCTCTCTCTCCCTCTCAACAAGAGTAGTATCTAGGGGGTAGAATATGCTATATCTATTTCCATACCAATAAAATTGGAAAATATATATATATAAAAAAAAAATTTATTACATGACAACTTGTAATAGAACACGGGTGAACTGATACATCCAAATATTTTTCACAAAATAATGAGTGTAATTCGAGAGATGTACCCAATTATTACTCTATTTTACCTGTTGTCTTTGAAAAAATCTATTGTGATGTATATACTTGTGAAAGCGGCAATTTTTATCACTATTTACAAAATTTTAACCCAAATTTTGTACCTAATGAGAAATTCTTTTATATAAAGTTATAGTTATTTTTCACAAAATTTTGTAAAAAAAAAAAAGAAAAAGGAGAGATAAATTAAGAAAAGGGAACAAAATTTAAATAATCACTTTTTTGAGGTAGGATGAATTATTATAAACATTTCGGTTATCAAAATGAAGTAACATTAAGGACCCATTTGGATTGATTTAAGAAAAAAGTGTTTATAAAAAAAGTTATTTTTATTTAAACTTTTTTGATAAAAATTGTTTAGAATACACCTTAAAATTTATTTTAAGTGGTTGGCAAATACTCCAATTTTTTTGTAAAATGACTTATTTTTAAAAATTAAACAATTGGATGTATTCCAAACAACTTTAAAGTAGGGGACAAAAAATAGTATTTGAACATAAATTAAATATTGTTTTTCCTTAGAAAGATATTGTATATATTTTGTGATTTTCCTTAAACTTCATAAAAAAATAATGGAAAAGACGGTGAAAATATTTCTGCCAAAGCCTACACACTATATAAAATCTAACAACAGAAACCAAAGACCAAGAAGAAGAAGAAAAAAAAAAAAAAAAAGAAAAGAAATTAAAGAGAAAAAAGGTGTCTTTATCTTTTATTTTTGGCCTCTTCAATATAATTTGTCTTCTATTTCTCCGATTGATTTGTCTTGTTATACAATGTATTTGATATCAGCTTCTTAAATTTTGACATAATTACATCAGACTGCAAACGTGGCCATGGACCCACCATTTCACGAGGCTTACAAGGCCCTGTTGGGCGAAACGGCGGCGATGGCGAAGGGAAGCGACATGGTGACGGTGGAGGAATGGGAGCTTCCGGTGGTTGATTTGAGCCGTTTGAAGGCGGCGGAAGCTGAAGAGTGTAAAAACGACATAATTAAAGCTTCTAAGGAATGGGGTTTTTTTCAAGTTGTGAATCATGGGATTTCGAATCAGCTTTTGGGTAAGATGAGAGCAAAACAAATCGAGCTTTTCAAACAGCCGTTTGAGCGTAAAAGCAAAGAGGATAAGTTTTTGAATTTCTCCGCCGGAAGTTACCGTTGGGGAACCCCCTCCGCCACTTCTCTCACCCAACTCTCTTGGTCTGAAGCTTTCCATGTTTCTCTCTCTGATATTCTTGGATCCAACGGCTCCGATGATCTCAGGTACAAATAATTATTTTTGTTTTTGAAAATTATGCTAATAAACCCCATTTTTAAAGAGATAAAAGAATAATATATGTACTTTTATATTGCCCATAATCGAGGGTAGGAACAGATTAAATTCTTTGATACGATACAATAGCATAGCATAGAGGCAAAAAAAAAATATATATATATTGTTGGTAATTGATAAAATAGTTTAATTAAATAAAATGCAAATATAATTCATCCATTCCAGGTCAACAATGGAAGAATTTGCGGGAAAAGTTTCGAGGTTAGCGCAAAAGCTAGCAGAGATTTTGGGAGAGAATTTAGATAGAAGTTCAAAATTCTTCGTTGAGAATTGTGTGCCGAGCACGTGCTATCTCCGAATGAACCGGTACCCGCCATGTCCGGTTTCGGGTCAAATATTCGGTCTGATGCCTCACACCGACAGCGATTTTTTGACGATTCTTCACCAAGACCAAGTGGGCGGCTTGGAGTTGGTGAAAGACGGGAAATGGATCGCCGTCAAACCCAATCCGGAGGCCCTTATTATAAATATCGGAGACTTATTTCAGGTAGTAATTGAATTAAATGAAATTATTATTATTATATTAAAAGGGAAAAGGGAAAATTAATGATAAAAAAGATTGTATTTAATTTTGGTTGGGTTTTGTTTTTTATTGGGGAAGTGCAGGCTTGGAGTAACGATGTTTACAAAAGCGTAGAGCATCGAGTTGTGACAAACTCGAAAGTGGAGAGATTTTCAATAGCGTATTTCTTGTGTCCGTGGAGTGAGACTGTAATTAAAAGCAAGTGCGAGCCTGGGGTTTATAGAAGGTTTAGTTTTAGGGAATTCAGGAATCAAGTTCAAGAGGATGTTCGGAAGCATGGCTACAAGATTGGTTTGCCTAGGTTTGCCTTATAATGATAACACAATTTTTTTCTTTTTTCTTTTTCCTTTTTTCTTTTAATATAAAATACTTGGATTGA

mRNA sequence

TGTGCTTACAAAATTTAGATATTGGGGAGTGGGTTTACGTTGAAGGTTCTTTGTCCATTCATTTCCCATTCCTTTCTTATCTTCCCCAACCATTCTCATAACTCTCAATTTCCCTATATATATAACTAAATATCCTCCCCACCTCATTCTCTCACACTCTTCTCCAATTATATATTCTCTATTTCCTTCCCCTTTCTCTTTTCATTTCCCTCTCATCGAAAGGGACATTTCCCCTTTTCCCAGACCATTTCCAGAACCAAATATGATGCACTCTCTAAACCAGACTGCAAACGTGGCCATGGACCCACCATTTCACGAGGCTTACAAGGCCCTGTTGGGCGAAACGGCGGCGATGGCGAAGGGAAGCGACATGGTGACGGTGGAGGAATGGGAGCTTCCGGTGGTTGATTTGAGCCGTTTGAAGGCGGCGGAAGCTGAAGAGTGTAAAAACGACATAATTAAAGCTTCTAAGGAATGGGGTTTTTTTCAAGTTGTGAATCATGGGATTTCGAATCAGCTTTTGGGTAAGATGAGAGCAAAACAAATCGAGCTTTTCAAACAGCCGTTTGAGCGTAAAAGCAAAGAGGATAAGTTTTTGAATTTCTCCGCCGGAAGTTACCGTTGGGGAACCCCCTCCGCCACTTCTCTCACCCAACTCTCTTGGTCTGAAGCTTTCCATGTTTCTCTCTCTGATATTCTTGGATCCAACGGCTCCGATGATCTCAGGTCAACAATGGAAGAATTTGCGGGAAAAGTTTCGAGGTTAGCGCAAAAGCTAGCAGAGATTTTGGGAGAGAATTTAGATAGAAGTTCAAAATTCTTCGTTGAGAATTGTGTGCCGAGCACGTGCTATCTCCGAATGAACCGGTACCCGCCATGTCCGGTTTCGGGTCAAATATTCGGTCTGATGCCTCACACCGACAGCGATTTTTTGACGATTCTTCACCAAGACCAAGTGGGCGGCTTGGAGTTGGTGAAAGACGGGAAATGGATCGCCGTCAAACCCAATCCGGAGGCCCTTATTATAAATATCGGAGACTTATTTCAGGCTTGGAGTAACGATGTTTACAAAAGCGTAGAGCATCGAGTTGTGACAAACTCGAAAGTGGAGAGATTTTCAATAGCGTATTTCTTGTGTCCGTGGAGTGAGACTGTAATTAAAAGCAAGTGCGAGCCTGGGGTTTATAGAAGGTTTAGTTTTAGGGAATTCAGGAATCAAGTTCAAGAGGATGTTCGGAAGCATGGCTACAAGATTGGTTTGCCTAGGTTTGCCTTATAATGATAACACAATTTTTTTCTTTTTTCTTTTTCCTTTTTTCTTTTAATATAAAATACTTGGATTGA

Coding sequence (CDS)

ATGATGCACTCTCTAAACCAGACTGCAAACGTGGCCATGGACCCACCATTTCACGAGGCTTACAAGGCCCTGTTGGGCGAAACGGCGGCGATGGCGAAGGGAAGCGACATGGTGACGGTGGAGGAATGGGAGCTTCCGGTGGTTGATTTGAGCCGTTTGAAGGCGGCGGAAGCTGAAGAGTGTAAAAACGACATAATTAAAGCTTCTAAGGAATGGGGTTTTTTTCAAGTTGTGAATCATGGGATTTCGAATCAGCTTTTGGGTAAGATGAGAGCAAAACAAATCGAGCTTTTCAAACAGCCGTTTGAGCGTAAAAGCAAAGAGGATAAGTTTTTGAATTTCTCCGCCGGAAGTTACCGTTGGGGAACCCCCTCCGCCACTTCTCTCACCCAACTCTCTTGGTCTGAAGCTTTCCATGTTTCTCTCTCTGATATTCTTGGATCCAACGGCTCCGATGATCTCAGGTCAACAATGGAAGAATTTGCGGGAAAAGTTTCGAGGTTAGCGCAAAAGCTAGCAGAGATTTTGGGAGAGAATTTAGATAGAAGTTCAAAATTCTTCGTTGAGAATTGTGTGCCGAGCACGTGCTATCTCCGAATGAACCGGTACCCGCCATGTCCGGTTTCGGGTCAAATATTCGGTCTGATGCCTCACACCGACAGCGATTTTTTGACGATTCTTCACCAAGACCAAGTGGGCGGCTTGGAGTTGGTGAAAGACGGGAAATGGATCGCCGTCAAACCCAATCCGGAGGCCCTTATTATAAATATCGGAGACTTATTTCAGGCTTGGAGTAACGATGTTTACAAAAGCGTAGAGCATCGAGTTGTGACAAACTCGAAAGTGGAGAGATTTTCAATAGCGTATTTCTTGTGTCCGTGGAGTGAGACTGTAATTAAAAGCAAGTGCGAGCCTGGGGTTTATAGAAGGTTTAGTTTTAGGGAATTCAGGAATCAAGTTCAAGAGGATGTTCGGAAGCATGGCTACAAGATTGGTTTGCCTAGGTTTGCCTTATAA

Protein sequence

MMHSLNQTANVAMDPPFHEAYKALLGETAAMAKGSDMVTVEEWELPVVDLSRLKAAEAEECKNDIIKASKEWGFFQVVNHGISNQLLGKMRAKQIELFKQPFERKSKEDKFLNFSAGSYRWGTPSATSLTQLSWSEAFHVSLSDILGSNGSDDLRSTMEEFAGKVSRLAQKLAEILGENLDRSSKFFVENCVPSTCYLRMNRYPPCPVSGQIFGLMPHTDSDFLTILHQDQVGGLELVKDGKWIAVKPNPEALIINIGDLFQAWSNDVYKSVEHRVVTNSKVERFSIAYFLCPWSETVIKSKCEPGVYRRFSFREFRNQVQEDVRKHGYKIGLPRFAL
Homology
BLAST of CaUC03G055000 vs. NCBI nr
Match: XP_038894099.1 (gibberellin 2-beta-dioxygenase 8 isoform X1 [Benincasa hispida])

HSP 1 Score: 646.4 bits (1666), Expect = 1.4e-181
Identity = 310/341 (90.91%), Postives = 328/341 (96.19%), Query Frame = 0

Query: 1   MMHSLNQTANVAMDPPFHEAYKALLGETAAMAKGSDMVTVEEWELPVVDLSRLK---AAE 60
           M+H+LNQ+ANVAMDPPFHEAYKALLGETAA+ +GS++VTVEEWELPVVDL RLK   A E
Sbjct: 1   MVHTLNQSANVAMDPPFHEAYKALLGETAAVPRGSEVVTVEEWELPVVDLRRLKAVAAVE 60

Query: 61  AEECKNDIIKASKEWGFFQVVNHGISNQLLGKMRAKQIELFKQPFERKSKEDKFLNFSAG 120
            EECK DIIKASKEWGFFQV+NHGISNQLL KMRAKQ+ELFKQPFERKSKED+FLNFSAG
Sbjct: 61  VEECKKDIIKASKEWGFFQVLNHGISNQLLAKMRAKQMELFKQPFERKSKEDQFLNFSAG 120

Query: 121 SYRWGTPSATSLTQLSWSEAFHVSLSDILGSNGSDDLRSTMEEFAGKVSRLAQKLAEILG 180
           SYRWGTPSATS+TQLSWSEAFHVSLSDILGSNGSD+LRSTMEEFAGKVSRLAQKLA ILG
Sbjct: 121 SYRWGTPSATSITQLSWSEAFHVSLSDILGSNGSDELRSTMEEFAGKVSRLAQKLAAILG 180

Query: 181 ENLDRSSKFFVENCVPSTCYLRMNRYPPCPVSGQIFGLMPHTDSDFLTILHQDQVGGLEL 240
           ENL RS+KFFVENCVPSTCYLRMNRYPPCPV GQIFGLMPHTDSDFLTILHQDQVGGLEL
Sbjct: 181 ENLGRSAKFFVENCVPSTCYLRMNRYPPCPVPGQIFGLMPHTDSDFLTILHQDQVGGLEL 240

Query: 241 VKDGKWIAVKPNPEALIINIGDLFQAWSNDVYKSVEHRVVTNSKVERFSIAYFLCPWSET 300
           VKDGKW+AVKPNP+ALI+NIGDLFQAWSNDVYKSVEHRVVTNSKVER+SIAYFLCPWSET
Sbjct: 241 VKDGKWVAVKPNPDALIVNIGDLFQAWSNDVYKSVEHRVVTNSKVERYSIAYFLCPWSET 300

Query: 301 VIKSKCEPGVYRRFSFREFRNQVQEDVRKHGYKIGLPRFAL 339
           VIKSKCEPGVYRRFSFREFRNQVQEDVRKHG+KIGLPRF L
Sbjct: 301 VIKSKCEPGVYRRFSFREFRNQVQEDVRKHGHKIGLPRFVL 341

BLAST of CaUC03G055000 vs. NCBI nr
Match: XP_023520137.1 (gibberellin 2-beta-dioxygenase 8 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 624.4 bits (1609), Expect = 5.7e-175
Identity = 300/342 (87.72%), Postives = 323/342 (94.44%), Query Frame = 0

Query: 1   MMHSLNQTANVAMDPPFHEAYKALLGETAAMAKGSDMVTVEEWELPVVDLSRLK----AA 60
           M+H+LNQ+ANVAMDPPFHEAYK+LL ET A+ +G D V V+EWELPVVDL RL+      
Sbjct: 1   MIHTLNQSANVAMDPPFHEAYKSLLAET-AVDRGGDAVAVKEWELPVVDLRRLRDGAEVG 60

Query: 61  EAEECKNDIIKASKEWGFFQVVNHGISNQLLGKMRAKQIELFKQPFERKSKEDKFLNFSA 120
           E E+CKNDII+AS+EWGFFQVVNHG+S++LLGKMRAKQIELFKQPFERKSKED FLNFSA
Sbjct: 61  EVEQCKNDIIRASQEWGFFQVVNHGVSSELLGKMRAKQIELFKQPFERKSKEDMFLNFSA 120

Query: 121 GSYRWGTPSATSLTQLSWSEAFHVSLSDILGSNGSDDLRSTMEEFAGKVSRLAQKLAEIL 180
           GSYRWGTP+ATSLTQLSWSEAFHVSLSDILGSNGSDDLRSTMEEFAGKVSRLAQ+LAEIL
Sbjct: 121 GSYRWGTPTATSLTQLSWSEAFHVSLSDILGSNGSDDLRSTMEEFAGKVSRLAQELAEIL 180

Query: 181 GENLDRSSKFFVENCVPSTCYLRMNRYPPCPVSGQIFGLMPHTDSDFLTILHQDQVGGLE 240
           GENL RSSKFFV+NCVPSTCYLRMNRYPPC V G++FGLMPHTDSDFLTILHQDQVGGLE
Sbjct: 181 GENLGRSSKFFVDNCVPSTCYLRMNRYPPCSVPGRVFGLMPHTDSDFLTILHQDQVGGLE 240

Query: 241 LVKDGKWIAVKPNPEALIINIGDLFQAWSNDVYKSVEHRVVTNSKVERFSIAYFLCPWSE 300
           LVKDG+WIAVKPNPEALIINIGDLFQAWSNDVYKSVEHRVVTNSK+ERFSIAYFLCPWSE
Sbjct: 241 LVKDGEWIAVKPNPEALIINIGDLFQAWSNDVYKSVEHRVVTNSKLERFSIAYFLCPWSE 300

Query: 301 TVIKSKCEPGVYRRFSFREFRNQVQEDVRKHGYKIGLPRFAL 339
           TVIKSKCEPGVYRRFSFREFRNQVQEDVRKHG+KIGLP+F L
Sbjct: 301 TVIKSKCEPGVYRRFSFREFRNQVQEDVRKHGFKIGLPKFVL 341

BLAST of CaUC03G055000 vs. NCBI nr
Match: XP_023001285.1 (gibberellin 2-beta-dioxygenase 8 [Cucurbita maxima])

HSP 1 Score: 621.3 bits (1601), Expect = 4.9e-174
Identity = 298/342 (87.13%), Postives = 323/342 (94.44%), Query Frame = 0

Query: 1   MMHSLNQTANVAMDPPFHEAYKALLGETAAMAKGSDMVTVEEWELPVVDLSRLK----AA 60
           M+H+LNQ+ANVAMDPPFHEAYK+LL ET A+ +G D V V+EWELPVVDL RL+    A 
Sbjct: 1   MIHTLNQSANVAMDPPFHEAYKSLLAET-AVDRGGDAVAVKEWELPVVDLRRLRDGAEAG 60

Query: 61  EAEECKNDIIKASKEWGFFQVVNHGISNQLLGKMRAKQIELFKQPFERKSKEDKFLNFSA 120
           E E+CKNDII+AS+EWGFFQVVNHG+S+Q+LGKMRAKQIELFKQPFERKSKED FLNFSA
Sbjct: 61  EVEQCKNDIIRASQEWGFFQVVNHGVSSQVLGKMRAKQIELFKQPFERKSKEDMFLNFSA 120

Query: 121 GSYRWGTPSATSLTQLSWSEAFHVSLSDILGSNGSDDLRSTMEEFAGKVSRLAQKLAEIL 180
           GSY WGTP+ATSLTQLSWSEAFH+SLSDILGSNGSDDLRSTMEEFAGKVSRLAQ+LAEIL
Sbjct: 121 GSYCWGTPTATSLTQLSWSEAFHISLSDILGSNGSDDLRSTMEEFAGKVSRLAQELAEIL 180

Query: 181 GENLDRSSKFFVENCVPSTCYLRMNRYPPCPVSGQIFGLMPHTDSDFLTILHQDQVGGLE 240
           GENL RSSKFFV+NCVPSTCYLRMNRYPPC V G++FGLMPHTDSDFLTILHQDQVGGLE
Sbjct: 181 GENLGRSSKFFVDNCVPSTCYLRMNRYPPCSVPGRVFGLMPHTDSDFLTILHQDQVGGLE 240

Query: 241 LVKDGKWIAVKPNPEALIINIGDLFQAWSNDVYKSVEHRVVTNSKVERFSIAYFLCPWSE 300
           LVKDG+WIAVKPNPEALIINIGDLFQAWSNDVYKSVEHRVVTNSK+ERFSIAYFLCPWSE
Sbjct: 241 LVKDGEWIAVKPNPEALIINIGDLFQAWSNDVYKSVEHRVVTNSKLERFSIAYFLCPWSE 300

Query: 301 TVIKSKCEPGVYRRFSFREFRNQVQEDVRKHGYKIGLPRFAL 339
           TVIKSKCEPGVY+RFSFREFRNQVQEDVRKHG+KIGLP+F L
Sbjct: 301 TVIKSKCEPGVYKRFSFREFRNQVQEDVRKHGFKIGLPKFVL 341

BLAST of CaUC03G055000 vs. NCBI nr
Match: XP_022933193.1 (gibberellin 2-beta-dioxygenase 8 [Cucurbita moschata])

HSP 1 Score: 619.8 bits (1597), Expect = 1.4e-173
Identity = 298/342 (87.13%), Postives = 321/342 (93.86%), Query Frame = 0

Query: 1   MMHSLNQTANVAMDPPFHEAYKALLGETAAMAKGSDMVTVEEWELPVVDLSRLK----AA 60
           M+H+LNQ+ANVAMDPPFHEAYK+LL ET A+ +G D V V+EWELPVVDL RL+      
Sbjct: 1   MIHTLNQSANVAMDPPFHEAYKSLLAET-AVERGGDAVAVKEWELPVVDLRRLRDGAEVG 60

Query: 61  EAEECKNDIIKASKEWGFFQVVNHGISNQLLGKMRAKQIELFKQPFERKSKEDKFLNFSA 120
           E E+CKNDII+AS+EWGFFQVVNHG+S+QLLGKMRAKQI LFKQPFERKSKED FLNFSA
Sbjct: 61  EVEQCKNDIIRASQEWGFFQVVNHGVSSQLLGKMRAKQIGLFKQPFERKSKEDMFLNFSA 120

Query: 121 GSYRWGTPSATSLTQLSWSEAFHVSLSDILGSNGSDDLRSTMEEFAGKVSRLAQKLAEIL 180
           GSYRWGTP+ATSLTQLSWSEAFHVSLSDILGSNGSDDLRSTMEEFAGKVSRLAQ+LAEIL
Sbjct: 121 GSYRWGTPTATSLTQLSWSEAFHVSLSDILGSNGSDDLRSTMEEFAGKVSRLAQELAEIL 180

Query: 181 GENLDRSSKFFVENCVPSTCYLRMNRYPPCPVSGQIFGLMPHTDSDFLTILHQDQVGGLE 240
           GENL RSSKFFV+NCVPSTCYLRMNRYPPC V G++FGLMPHTDSDFLTILHQDQVGGLE
Sbjct: 181 GENLGRSSKFFVDNCVPSTCYLRMNRYPPCSVPGRVFGLMPHTDSDFLTILHQDQVGGLE 240

Query: 241 LVKDGKWIAVKPNPEALIINIGDLFQAWSNDVYKSVEHRVVTNSKVERFSIAYFLCPWSE 300
           LVKDG+WIAVKPNPEALIINIGDLFQAWSN+VYKSVEHRVVTNSK+ERFSIAYF CPWSE
Sbjct: 241 LVKDGEWIAVKPNPEALIINIGDLFQAWSNNVYKSVEHRVVTNSKLERFSIAYFFCPWSE 300

Query: 301 TVIKSKCEPGVYRRFSFREFRNQVQEDVRKHGYKIGLPRFAL 339
           TVIKSKCEPGVYRRFSFREFRNQVQEDVRKHG+KIGLP+F L
Sbjct: 301 TVIKSKCEPGVYRRFSFREFRNQVQEDVRKHGFKIGLPKFVL 341

BLAST of CaUC03G055000 vs. NCBI nr
Match: XP_008441451.1 (PREDICTED: gibberellin 2-beta-dioxygenase 8 isoform X2 [Cucumis melo])

HSP 1 Score: 614.8 bits (1584), Expect = 4.6e-172
Identity = 305/345 (88.41%), Postives = 317/345 (91.88%), Query Frame = 0

Query: 1   MMHSLNQ-TANVAMDPPFHEAYKALLGETAAMAKG-SDMVTVEEWELPVVDLSRL---KA 60
           M H L Q +ANVAMDPPFHEAYK LL ETAA AKG SD+VTVEEW+LPVVDL RL   K 
Sbjct: 1   MTHILKQSSANVAMDPPFHEAYKTLLAETAAEAKGTSDVVTVEEWDLPVVDLGRLTGGKV 60

Query: 61  AEAEECKNDIIKASKEWGFFQVVNHGISNQLLGKMRAKQIELFKQPFERKSKEDKFLNFS 120
            E EECKNDII+ASKEWGFFQVVNHGISNQLL KMRAKQIELFKQPFERKSKED+F NFS
Sbjct: 61  EEEEECKNDIIRASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFS 120

Query: 121 AGSYRWGTPSATSLTQLSWSEAFHVSLSDILGSN--GSDDLRSTMEEFAGKVSRLAQKLA 180
           AGSYRWGTPSATS+TQLSWSEAFHVSLSDILGSN    DDLRSTMEE+AGKVSRLAQKLA
Sbjct: 121 AGSYRWGTPSATSITQLSWSEAFHVSLSDILGSNYGSDDDLRSTMEEYAGKVSRLAQKLA 180

Query: 181 EILGENLDRSSKFFVENCVPSTCYLRMNRYPPCPVSGQIFGLMPHTDSDFLTILHQDQVG 240
           EILGENL RSSKFFVENCVPSTCYLRMNRYPPCPV GQIFGLMPHTDSDFLTILHQDQVG
Sbjct: 181 EILGENLGRSSKFFVENCVPSTCYLRMNRYPPCPVQGQIFGLMPHTDSDFLTILHQDQVG 240

Query: 241 GLELVKDGKWIAVKPNPEALIINIGDLFQAWSNDVYKSVEHRVVTNSKVERFSIAYFLCP 300
           GLELVKDGKWIAVKPNP+ALI+NIGDLFQ WSND YKSVEHRVVTNS+ ER+SIAYFLCP
Sbjct: 241 GLELVKDGKWIAVKPNPQALIVNIGDLFQVWSNDEYKSVEHRVVTNSRKERYSIAYFLCP 300

Query: 301 WSETVIKSKCEPGVYRRFSFREFRNQVQEDVRKHGYKIGLPRFAL 339
           WSETVIKSK EPGVYRRFSFREFRNQVQEDVRK+GYKIGLPRF L
Sbjct: 301 WSETVIKSKSEPGVYRRFSFREFRNQVQEDVRKYGYKIGLPRFVL 345

BLAST of CaUC03G055000 vs. ExPASy Swiss-Prot
Match: O49561 (Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis thaliana OX=3702 GN=GA2OX8 PE=1 SV=2)

HSP 1 Score: 421.0 bits (1081), Expect = 1.3e-116
Identity = 208/337 (61.72%), Postives = 252/337 (74.78%), Query Frame = 0

Query: 13  MDPPFHEAYKALLGETAAMAKGSDM----------VTVEEWELPVVDLSRL---KAAEAE 72
           MDPPF+E Y  LL       K +D+            VEE ELPV+D+SRL      E E
Sbjct: 1   MDPPFNEIYNNLL-YNQITKKDNDVSEIPFSFSVTAVVEEVELPVIDVSRLIDGAEEERE 60

Query: 73  ECKNDIIKASKEWGFFQVVNHGISNQLLGKMRAKQIELFKQPFERKSKEDKFLNFSAGSY 132
           +CK  I +AS+EWGFFQV+NHGIS  +L KMR +QI +F++PF++KSK +K   FSAGSY
Sbjct: 61  KCKEAIARASREWGFFQVINHGISMDVLEKMRQEQIRVFREPFDKKSKSEK---FSAGSY 120

Query: 133 RWGTPSATSLTQLSWSEAFHVSLSDILGSNGSDDLRSTMEEFAGKVSRLAQKLAEILGEN 192
           RWGTPSATS+ QLSWSEAFHV ++DI  +     L STME+FA +   LA  LAE+L E 
Sbjct: 121 RWGTPSATSIRQLSWSEAFHVPMTDISDNKDFTTLSSTMEKFASESEALAYMLAEVLAEK 180

Query: 193 LDRSSKFFVENCVPSTCYLRMNRYPPCPVSGQIFGLMPHTDSDFLTILHQDQVGGLELVK 252
             ++S FF ENCV +TCYLRMNRYPPCP   +++GLMPHTDSDFLTIL+QDQVGGL+L+K
Sbjct: 181 SGQNSSFFKENCVRNTCYLRMNRYPPCPKPSEVYGLMPHTDSDFLTILYQDQVGGLQLIK 240

Query: 253 DGKWIAVKPNPEALIINIGDLFQAWSNDVYKSVEHRVVTNSKVERFSIAYFLCPWSETVI 312
           D +WIAVKPNP+ALIINIGDLFQAWSN +YKSVEHRV+TN KVERFS AYF+CP  + VI
Sbjct: 241 DNRWIAVKPNPKALIINIGDLFQAWSNGMYKSVEHRVMTNPKVERFSTAYFMCPSYDAVI 300

Query: 313 KSKCEPGVYRRFSFREFRNQVQEDVRKHGYKIGLPRF 337
           +   +   YR FSFREFR QVQEDV+K G+K+GLPRF
Sbjct: 301 ECSSDRPAYRNFSFREFRQQVQEDVKKFGFKVGLPRF 333

BLAST of CaUC03G055000 vs. ExPASy Swiss-Prot
Match: Q7XP65 (Gibberellin 2-beta-dioxygenase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2OX6 PE=1 SV=1)

HSP 1 Score: 334.7 bits (857), Expect = 1.2e-90
Identity = 172/352 (48.86%), Postives = 230/352 (65.34%), Query Frame = 0

Query: 9   ANVAMDPPFHEAYKAL----------LGETAAMAKGSDMVTVEEWELPVVDLSRLK---A 68
           A++A+DPP  ++Y+AL          +   A    GS    +E  +LP+VDL RL    A
Sbjct: 5   ADIAIDPPLADSYRALALLRRDRDGGIAPPAVQMVGSGGAVLER-DLPMVDLERLTRGGA 64

Query: 69  AEAEECKNDIIKASKEWGFFQVVNHGISNQLLGKMRAKQIELFKQPFERKSKEDKFLNFS 128
            E + C   + +A+ EWGFFQ+ NHG+  +L+ +MR +Q  LF+ PFE K K    LN  
Sbjct: 65  GERKACAGAMARAASEWGFFQLTNHGVGRELMEEMRREQARLFRLPFETKEKAG-LLN-- 124

Query: 129 AGSYRWGTPSATSLTQLSWSEAFHVSLSDILGSN----GSDDLRSTMEEFAGKVSRLAQK 188
            GSYRWG P+ATSL  LSWSEAFHV L+ I G++        LR  M+E A  +SR+A  
Sbjct: 125 -GSYRWGNPTATSLRHLSWSEAFHVPLASISGADCDFGDLTSLRGVMQEVAEAMSRVANT 184

Query: 189 LAEILGENLD-------RSSKFFVENCVPSTCYLRMNRYPPCPVSGQIFGLMPHTDSDFL 248
           +A  L E L         ++ +F   C  +TC+LR+NRYP CP +   FGL+PHTDSDFL
Sbjct: 185 VAAALAEELTGRGGGGASAAPWFPAGCDETTCFLRLNRYPACPFAADTFGLVPHTDSDFL 244

Query: 249 TILHQDQVGGLELVKDGKWIAVKPNPEALIINIGDLFQAWSNDVYKSVEHRVVTNSKVER 308
           T+L QDQVGGL L+KD +W+AV+P P+AL++NIGDLFQAWSN+ YKSVEH+VV N+K +R
Sbjct: 245 TVLCQDQVGGLHLMKDSRWVAVRPRPDALVVNIGDLFQAWSNNRYKSVEHKVVANAKTDR 304

Query: 309 FSIAYFLCPWSETVIKSKCEPGVYRRFSFREFRNQVQEDVRKHGYKIGLPRF 337
            S+AYFLCP  ++++ +  EP  YR F+F E+R +VQEDVR  G KIGLP F
Sbjct: 305 LSVAYFLCPSYDSLVGTCGEPSPYRAFTFGEYRKKVQEDVRTTGKKIGLPNF 351

BLAST of CaUC03G055000 vs. ExPASy Swiss-Prot
Match: Q8LGZ9 (Gibberellin 2-beta-dioxygenase 5 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2OX5 PE=1 SV=1)

HSP 1 Score: 310.8 bits (795), Expect = 1.8e-83
Identity = 164/333 (49.25%), Postives = 219/333 (65.77%), Query Frame = 0

Query: 14  DPPFHEAYKALLGETAAMAKGSDMVTVEEWELPVVDLSRLKAAEAEECKNDIIKASKEWG 73
           +PP    YK L  E   +      + V+E ELPV+DL+ L  AE + C+ D+++A+ EWG
Sbjct: 11  NPPLMSTYKHLFVEQHRLDMDMGAIDVDECELPVIDLAGLMEAE-QVCRADMVRAASEWG 70

Query: 74  FFQVVNHGISNQLLGKMRAKQIELFKQPFERKSKEDKFLNFSAGSYRWGTPSATSLTQLS 133
           FFQV NHG+   LL ++   Q+ +F++PF+ K  E + L FS  SYRWGTP+A  L QLS
Sbjct: 71  FFQVTNHGVPQALLRELHDAQVAVFRRPFQEKVTE-RLLGFSPESYRWGTPTAKCLEQLS 130

Query: 134 WSEAFHVSLSDILGSNGSDDLRSTMEEFAGKVSRLAQKLAEILGENLDRSSKFFVENCVP 193
           WSEA+H+ ++    S  S   R+ +EE +  +  LAQKLAEIL   L  + +   E  V 
Sbjct: 131 WSEAYHIPMTTPRPST-SIRARAVIEEVSRAMYELAQKLAEILMRGLPGAGE--GETMVT 190

Query: 194 S---TCYLRMNRYPPCPVSGQIFGLMPHTDSDFLTILH--QDQVGGLELVKDGKWIAVKP 253
           +   TC+LR+NRYPPC ++   FGL PHTDSD LTI+H  QD VGGL+L+K G+W+AVKP
Sbjct: 191 TREETCFLRLNRYPPCAMAMGGFGLCPHTDSDLLTIVHQQQDTVGGLQLLKGGRWVAVKP 250

Query: 254 NPEALIINIGDLFQAWSNDVYKSVEHRVVTNSKVERFSIAYFLCPWSETVIKSKC----- 313
           +P  LI+N+GDL QAWSNDVYKSVEHRV+ N+ +ERFS+A+FLCP   T+I         
Sbjct: 251 SPSTLIVNVGDLLQAWSNDVYKSVEHRVMANATLERFSMAFFLCPSYHTLIIPSSSHVHD 310

Query: 314 EPGVYRRFSFREFRNQVQEDVRKHGYKIGLPRF 337
           +   YR F+F E+R Q+ EDVR  G KIGL RF
Sbjct: 311 DDAHYRSFTFGEYRKQIMEDVRSTGRKIGLHRF 338

BLAST of CaUC03G055000 vs. ExPASy Swiss-Prot
Match: Q9C6I4 (Gibberellin 2-beta-dioxygenase 7 OS=Arabidopsis thaliana OX=3702 GN=GA2OX7 PE=1 SV=1)

HSP 1 Score: 295.4 bits (755), Expect = 8.0e-79
Identity = 150/337 (44.51%), Postives = 219/337 (64.99%), Query Frame = 0

Query: 11  VAMDPPFHEAYKALLG-----ETAAMAKGSDMVTVEEWELPVVDLSRLKAAE---AEECK 70
           +A  PPF   + ++ G      T+     +  +     +LPV+DLS L + E    + C 
Sbjct: 1   MASQPPFKTNFCSIFGSSFPNSTSESNTNTSTIQTSGIKLPVIDLSHLTSGEEVKRKRCV 60

Query: 71  NDIIKASKEWGFFQVVNHGISNQLLGKMRAKQIELFKQPFERKSKEDKFLNFSAGSYRWG 130
             ++ A+KEWGFFQ+VNHGI   +   M  ++ +LF QPF  K +E +F + S  SYRWG
Sbjct: 61  KQMVAAAKEWGFFQIVNHGIPKDVFEMMLLEEKKLFDQPFSVKVRE-RFSDLSKNSYRWG 120

Query: 131 TPSATSLTQLSWSEAFHVSLSDILG-SNGSDDLRSTMEEFAGKVSRLAQKLAEILGENLD 190
            PSATS  Q S SEAFH+ LS++   S+  ++LR+ +E +  +++R+AQ + EILG+ ++
Sbjct: 121 NPSATSPAQYSVSEAFHIILSEVSRISDDRNNLRTIVETYVQEIARVAQMICEILGKQVN 180

Query: 191 RSSKFFVENCVPSTCYLRMNRYPPCPVSGQIFGLMPHTDSDFLTILHQDQVGGLELVKDG 250
            SS++F         +LR+N+Y P     ++FGL+PHTD+ FLTIL QDQ+GGLEL  +G
Sbjct: 181 VSSEYFENIFELENSFLRLNKYHPSVFGSEVFGLVPHTDTSFLTILSQDQIGGLELENNG 240

Query: 251 KWIAVKPNPEALIINIGDLFQAWSNDVYKSVEHRVVTNSKVERFSIAYFLCPWSETVIKS 310
           +WI+VKP  EAL +NIGD+FQA SN VY+SV HRV++ + +ER SIA+F+CP+ ET I  
Sbjct: 241 QWISVKPCLEALTVNIGDMFQALSNGVYQSVRHRVISPANIERMSIAFFVCPYLETEIDC 300

Query: 311 KCEPGVYRRFSFREFRNQVQEDVRKHGYKIGLPRFAL 339
              P  YRRFSFRE++ Q + DV++ G K+GL RF +
Sbjct: 301 FGYPKKYRRFSFREYKEQSEHDVKETGDKVGLSRFLI 336

BLAST of CaUC03G055000 vs. ExPASy Swiss-Prot
Match: Q39112 (Gibberellin 20 oxidase 3 OS=Arabidopsis thaliana OX=3702 GN=GA20OX3 PE=2 SV=1)

HSP 1 Score: 162.2 bits (409), Expect = 1.1e-38
Identity = 97/285 (34.04%), Postives = 141/285 (49.47%), Query Frame = 0

Query: 40  VEEWELPVVDLSRLKAAE---AEECKNDIIKASKEWGFFQVVNHGISNQLLGKMRAKQIE 99
           V+  ++P++DL+   + +   A E    + KA+ + GFF + NHG+   LL +       
Sbjct: 53  VQPLQVPLIDLAGFLSGDSCLASEATRLVSKAATKHGFFLITNHGVDESLLSRAYLHMDS 112

Query: 100 LFKQPFERKSKEDKFLNFSAGSYRWGTPS--ATSL-----TQLSWSEAFHVSLS------ 159
            FK P   K K  +         +WG  S  A+S      ++L W E      S      
Sbjct: 113 FFKAPACEKQKAQR---------KWGESSGYASSFVGRFSSKLPWKETLSFKFSPEEKIH 172

Query: 160 -----DILG---SNGSDDLRSTMEEFAGKVSRLAQKLAEILGENLDRSSKFFVENCVPST 219
                D +     +G +D     +E+A  ++ L+ K+ E+LG +L    ++F E    S 
Sbjct: 173 SQTVKDFVSKKMGDGYEDFGKVYQEYAEAMNTLSLKIMELLGMSLGVERRYFKEFFEDSD 232

Query: 220 CYLRMNRYPPCPVSGQIFGLMPHTDSDFLTILHQDQVGGLELVKDGKWIAVKPNPEALII 279
              R+N YP C       G  PH D   LTILHQDQVGGL++  D KW ++ PNP A ++
Sbjct: 233 SIFRLNYYPQCKQPELALGTGPHCDPTSLTILHQDQVGGLQVFVDNKWQSIPPNPHAFVV 292

Query: 280 NIGDLFQAWSNDVYKSVEHRVVTNSKVERFSIAYFLCPWSETVIK 301
           NIGD F A +N  YKS  HR V NS+ ER + A+FLCP  E V+K
Sbjct: 293 NIGDTFMALTNGRYKSCLHRAVVNSERERKTFAFFLCPKGEKVVK 328

BLAST of CaUC03G055000 vs. ExPASy TrEMBL
Match: A0A6J1KKS1 (gibberellin 2-beta-dioxygenase 8 OS=Cucurbita maxima OX=3661 GN=LOC111495461 PE=3 SV=1)

HSP 1 Score: 621.3 bits (1601), Expect = 2.4e-174
Identity = 298/342 (87.13%), Postives = 323/342 (94.44%), Query Frame = 0

Query: 1   MMHSLNQTANVAMDPPFHEAYKALLGETAAMAKGSDMVTVEEWELPVVDLSRLK----AA 60
           M+H+LNQ+ANVAMDPPFHEAYK+LL ET A+ +G D V V+EWELPVVDL RL+    A 
Sbjct: 1   MIHTLNQSANVAMDPPFHEAYKSLLAET-AVDRGGDAVAVKEWELPVVDLRRLRDGAEAG 60

Query: 61  EAEECKNDIIKASKEWGFFQVVNHGISNQLLGKMRAKQIELFKQPFERKSKEDKFLNFSA 120
           E E+CKNDII+AS+EWGFFQVVNHG+S+Q+LGKMRAKQIELFKQPFERKSKED FLNFSA
Sbjct: 61  EVEQCKNDIIRASQEWGFFQVVNHGVSSQVLGKMRAKQIELFKQPFERKSKEDMFLNFSA 120

Query: 121 GSYRWGTPSATSLTQLSWSEAFHVSLSDILGSNGSDDLRSTMEEFAGKVSRLAQKLAEIL 180
           GSY WGTP+ATSLTQLSWSEAFH+SLSDILGSNGSDDLRSTMEEFAGKVSRLAQ+LAEIL
Sbjct: 121 GSYCWGTPTATSLTQLSWSEAFHISLSDILGSNGSDDLRSTMEEFAGKVSRLAQELAEIL 180

Query: 181 GENLDRSSKFFVENCVPSTCYLRMNRYPPCPVSGQIFGLMPHTDSDFLTILHQDQVGGLE 240
           GENL RSSKFFV+NCVPSTCYLRMNRYPPC V G++FGLMPHTDSDFLTILHQDQVGGLE
Sbjct: 181 GENLGRSSKFFVDNCVPSTCYLRMNRYPPCSVPGRVFGLMPHTDSDFLTILHQDQVGGLE 240

Query: 241 LVKDGKWIAVKPNPEALIINIGDLFQAWSNDVYKSVEHRVVTNSKVERFSIAYFLCPWSE 300
           LVKDG+WIAVKPNPEALIINIGDLFQAWSNDVYKSVEHRVVTNSK+ERFSIAYFLCPWSE
Sbjct: 241 LVKDGEWIAVKPNPEALIINIGDLFQAWSNDVYKSVEHRVVTNSKLERFSIAYFLCPWSE 300

Query: 301 TVIKSKCEPGVYRRFSFREFRNQVQEDVRKHGYKIGLPRFAL 339
           TVIKSKCEPGVY+RFSFREFRNQVQEDVRKHG+KIGLP+F L
Sbjct: 301 TVIKSKCEPGVYKRFSFREFRNQVQEDVRKHGFKIGLPKFVL 341

BLAST of CaUC03G055000 vs. ExPASy TrEMBL
Match: A0A6J1F422 (gibberellin 2-beta-dioxygenase 8 OS=Cucurbita moschata OX=3662 GN=LOC111440045 PE=3 SV=1)

HSP 1 Score: 619.8 bits (1597), Expect = 6.8e-174
Identity = 298/342 (87.13%), Postives = 321/342 (93.86%), Query Frame = 0

Query: 1   MMHSLNQTANVAMDPPFHEAYKALLGETAAMAKGSDMVTVEEWELPVVDLSRLK----AA 60
           M+H+LNQ+ANVAMDPPFHEAYK+LL ET A+ +G D V V+EWELPVVDL RL+      
Sbjct: 1   MIHTLNQSANVAMDPPFHEAYKSLLAET-AVERGGDAVAVKEWELPVVDLRRLRDGAEVG 60

Query: 61  EAEECKNDIIKASKEWGFFQVVNHGISNQLLGKMRAKQIELFKQPFERKSKEDKFLNFSA 120
           E E+CKNDII+AS+EWGFFQVVNHG+S+QLLGKMRAKQI LFKQPFERKSKED FLNFSA
Sbjct: 61  EVEQCKNDIIRASQEWGFFQVVNHGVSSQLLGKMRAKQIGLFKQPFERKSKEDMFLNFSA 120

Query: 121 GSYRWGTPSATSLTQLSWSEAFHVSLSDILGSNGSDDLRSTMEEFAGKVSRLAQKLAEIL 180
           GSYRWGTP+ATSLTQLSWSEAFHVSLSDILGSNGSDDLRSTMEEFAGKVSRLAQ+LAEIL
Sbjct: 121 GSYRWGTPTATSLTQLSWSEAFHVSLSDILGSNGSDDLRSTMEEFAGKVSRLAQELAEIL 180

Query: 181 GENLDRSSKFFVENCVPSTCYLRMNRYPPCPVSGQIFGLMPHTDSDFLTILHQDQVGGLE 240
           GENL RSSKFFV+NCVPSTCYLRMNRYPPC V G++FGLMPHTDSDFLTILHQDQVGGLE
Sbjct: 181 GENLGRSSKFFVDNCVPSTCYLRMNRYPPCSVPGRVFGLMPHTDSDFLTILHQDQVGGLE 240

Query: 241 LVKDGKWIAVKPNPEALIINIGDLFQAWSNDVYKSVEHRVVTNSKVERFSIAYFLCPWSE 300
           LVKDG+WIAVKPNPEALIINIGDLFQAWSN+VYKSVEHRVVTNSK+ERFSIAYF CPWSE
Sbjct: 241 LVKDGEWIAVKPNPEALIINIGDLFQAWSNNVYKSVEHRVVTNSKLERFSIAYFFCPWSE 300

Query: 301 TVIKSKCEPGVYRRFSFREFRNQVQEDVRKHGYKIGLPRFAL 339
           TVIKSKCEPGVYRRFSFREFRNQVQEDVRKHG+KIGLP+F L
Sbjct: 301 TVIKSKCEPGVYRRFSFREFRNQVQEDVRKHGFKIGLPKFVL 341

BLAST of CaUC03G055000 vs. ExPASy TrEMBL
Match: A0A1S3B309 (gibberellin 2-beta-dioxygenase 8 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103485556 PE=3 SV=1)

HSP 1 Score: 614.8 bits (1584), Expect = 2.2e-172
Identity = 305/345 (88.41%), Postives = 317/345 (91.88%), Query Frame = 0

Query: 1   MMHSLNQ-TANVAMDPPFHEAYKALLGETAAMAKG-SDMVTVEEWELPVVDLSRL---KA 60
           M H L Q +ANVAMDPPFHEAYK LL ETAA AKG SD+VTVEEW+LPVVDL RL   K 
Sbjct: 1   MTHILKQSSANVAMDPPFHEAYKTLLAETAAEAKGTSDVVTVEEWDLPVVDLGRLTGGKV 60

Query: 61  AEAEECKNDIIKASKEWGFFQVVNHGISNQLLGKMRAKQIELFKQPFERKSKEDKFLNFS 120
            E EECKNDII+ASKEWGFFQVVNHGISNQLL KMRAKQIELFKQPFERKSKED+F NFS
Sbjct: 61  EEEEECKNDIIRASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFS 120

Query: 121 AGSYRWGTPSATSLTQLSWSEAFHVSLSDILGSN--GSDDLRSTMEEFAGKVSRLAQKLA 180
           AGSYRWGTPSATS+TQLSWSEAFHVSLSDILGSN    DDLRSTMEE+AGKVSRLAQKLA
Sbjct: 121 AGSYRWGTPSATSITQLSWSEAFHVSLSDILGSNYGSDDDLRSTMEEYAGKVSRLAQKLA 180

Query: 181 EILGENLDRSSKFFVENCVPSTCYLRMNRYPPCPVSGQIFGLMPHTDSDFLTILHQDQVG 240
           EILGENL RSSKFFVENCVPSTCYLRMNRYPPCPV GQIFGLMPHTDSDFLTILHQDQVG
Sbjct: 181 EILGENLGRSSKFFVENCVPSTCYLRMNRYPPCPVQGQIFGLMPHTDSDFLTILHQDQVG 240

Query: 241 GLELVKDGKWIAVKPNPEALIINIGDLFQAWSNDVYKSVEHRVVTNSKVERFSIAYFLCP 300
           GLELVKDGKWIAVKPNP+ALI+NIGDLFQ WSND YKSVEHRVVTNS+ ER+SIAYFLCP
Sbjct: 241 GLELVKDGKWIAVKPNPQALIVNIGDLFQVWSNDEYKSVEHRVVTNSRKERYSIAYFLCP 300

Query: 301 WSETVIKSKCEPGVYRRFSFREFRNQVQEDVRKHGYKIGLPRFAL 339
           WSETVIKSK EPGVYRRFSFREFRNQVQEDVRK+GYKIGLPRF L
Sbjct: 301 WSETVIKSKSEPGVYRRFSFREFRNQVQEDVRKYGYKIGLPRFVL 345

BLAST of CaUC03G055000 vs. ExPASy TrEMBL
Match: A0A1S3B3F3 (gibberellin 2-beta-dioxygenase 8 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103485556 PE=3 SV=1)

HSP 1 Score: 612.1 bits (1577), Expect = 1.4e-171
Identity = 301/337 (89.32%), Postives = 313/337 (92.88%), Query Frame = 0

Query: 8   TANVAMDPPFHEAYKALLGETAAMAKG-SDMVTVEEWELPVVDLSRL---KAAEAEECKN 67
           +ANVAMDPPFHEAYK LL ETAA AKG SD+VTVEEW+LPVVDL RL   K  E EECKN
Sbjct: 41  SANVAMDPPFHEAYKTLLAETAAEAKGTSDVVTVEEWDLPVVDLGRLTGGKVEEEEECKN 100

Query: 68  DIIKASKEWGFFQVVNHGISNQLLGKMRAKQIELFKQPFERKSKEDKFLNFSAGSYRWGT 127
           DII+ASKEWGFFQVVNHGISNQLL KMRAKQIELFKQPFERKSKED+F NFSAGSYRWGT
Sbjct: 101 DIIRASKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWGT 160

Query: 128 PSATSLTQLSWSEAFHVSLSDILGSN--GSDDLRSTMEEFAGKVSRLAQKLAEILGENLD 187
           PSATS+TQLSWSEAFHVSLSDILGSN    DDLRSTMEE+AGKVSRLAQKLAEILGENL 
Sbjct: 161 PSATSITQLSWSEAFHVSLSDILGSNYGSDDDLRSTMEEYAGKVSRLAQKLAEILGENLG 220

Query: 188 RSSKFFVENCVPSTCYLRMNRYPPCPVSGQIFGLMPHTDSDFLTILHQDQVGGLELVKDG 247
           RSSKFFVENCVPSTCYLRMNRYPPCPV GQIFGLMPHTDSDFLTILHQDQVGGLELVKDG
Sbjct: 221 RSSKFFVENCVPSTCYLRMNRYPPCPVQGQIFGLMPHTDSDFLTILHQDQVGGLELVKDG 280

Query: 248 KWIAVKPNPEALIINIGDLFQAWSNDVYKSVEHRVVTNSKVERFSIAYFLCPWSETVIKS 307
           KWIAVKPNP+ALI+NIGDLFQ WSND YKSVEHRVVTNS+ ER+SIAYFLCPWSETVIKS
Sbjct: 281 KWIAVKPNPQALIVNIGDLFQVWSNDEYKSVEHRVVTNSRKERYSIAYFLCPWSETVIKS 340

Query: 308 KCEPGVYRRFSFREFRNQVQEDVRKHGYKIGLPRFAL 339
           K EPGVYRRFSFREFRNQVQEDVRK+GYKIGLPRF L
Sbjct: 341 KSEPGVYRRFSFREFRNQVQEDVRKYGYKIGLPRFVL 377

BLAST of CaUC03G055000 vs. ExPASy TrEMBL
Match: A0A5A7UM12 (Gibberellin 2-beta-dioxygenase 8 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G006030 PE=3 SV=1)

HSP 1 Score: 604.7 bits (1558), Expect = 2.3e-169
Identity = 297/332 (89.46%), Postives = 308/332 (92.77%), Query Frame = 0

Query: 13  MDPPFHEAYKALLGETAAMAKG-SDMVTVEEWELPVVDLSRL---KAAEAEECKNDIIKA 72
           MDPPFHEAYK LL ETAA AKG SD+VTVEEW+LPVVDL RL   K  E EECKNDII+A
Sbjct: 1   MDPPFHEAYKTLLAETAAEAKGTSDVVTVEEWDLPVVDLGRLTGGKVEEEEECKNDIIRA 60

Query: 73  SKEWGFFQVVNHGISNQLLGKMRAKQIELFKQPFERKSKEDKFLNFSAGSYRWGTPSATS 132
           SKEWGFFQVVNHGISNQLL KMRAKQIELFKQPFERKSKED+F NFSAGSYRWGTPSATS
Sbjct: 61  SKEWGFFQVVNHGISNQLLAKMRAKQIELFKQPFERKSKEDQFSNFSAGSYRWGTPSATS 120

Query: 133 LTQLSWSEAFHVSLSDILGSN--GSDDLRSTMEEFAGKVSRLAQKLAEILGENLDRSSKF 192
           +TQLSWSEAFHVSLSDILGSN    DDLRSTMEE+AGKVSRLAQKLAEILGENL RSSKF
Sbjct: 121 ITQLSWSEAFHVSLSDILGSNYGSDDDLRSTMEEYAGKVSRLAQKLAEILGENLGRSSKF 180

Query: 193 FVENCVPSTCYLRMNRYPPCPVSGQIFGLMPHTDSDFLTILHQDQVGGLELVKDGKWIAV 252
           FVENCVPSTCYLRMNRYPPCPV GQIFGLMPHTDSDFLTILHQDQVGGLELVKDGKWIAV
Sbjct: 181 FVENCVPSTCYLRMNRYPPCPVQGQIFGLMPHTDSDFLTILHQDQVGGLELVKDGKWIAV 240

Query: 253 KPNPEALIINIGDLFQAWSNDVYKSVEHRVVTNSKVERFSIAYFLCPWSETVIKSKCEPG 312
           KPNP+ALI+NIGDLFQ WSND YKSVEHRVVTNS+ ER+SIAYFLCPWSETVIKSK EPG
Sbjct: 241 KPNPQALIVNIGDLFQVWSNDEYKSVEHRVVTNSRKERYSIAYFLCPWSETVIKSKSEPG 300

Query: 313 VYRRFSFREFRNQVQEDVRKHGYKIGLPRFAL 339
           VYRRFSFREFRNQVQEDVRK+GYKIGLPRF L
Sbjct: 301 VYRRFSFREFRNQVQEDVRKYGYKIGLPRFVL 332

BLAST of CaUC03G055000 vs. TAIR 10
Match: AT4G21200.1 (gibberellin 2-oxidase 8 )

HSP 1 Score: 421.0 bits (1081), Expect = 9.0e-118
Identity = 208/337 (61.72%), Postives = 252/337 (74.78%), Query Frame = 0

Query: 13  MDPPFHEAYKALLGETAAMAKGSDM----------VTVEEWELPVVDLSRL---KAAEAE 72
           MDPPF+E Y  LL       K +D+            VEE ELPV+D+SRL      E E
Sbjct: 1   MDPPFNEIYNNLL-YNQITKKDNDVSEIPFSFSVTAVVEEVELPVIDVSRLIDGAEEERE 60

Query: 73  ECKNDIIKASKEWGFFQVVNHGISNQLLGKMRAKQIELFKQPFERKSKEDKFLNFSAGSY 132
           +CK  I +AS+EWGFFQV+NHGIS  +L KMR +QI +F++PF++KSK +K   FSAGSY
Sbjct: 61  KCKEAIARASREWGFFQVINHGISMDVLEKMRQEQIRVFREPFDKKSKSEK---FSAGSY 120

Query: 133 RWGTPSATSLTQLSWSEAFHVSLSDILGSNGSDDLRSTMEEFAGKVSRLAQKLAEILGEN 192
           RWGTPSATS+ QLSWSEAFHV ++DI  +     L STME+FA +   LA  LAE+L E 
Sbjct: 121 RWGTPSATSIRQLSWSEAFHVPMTDISDNKDFTTLSSTMEKFASESEALAYMLAEVLAEK 180

Query: 193 LDRSSKFFVENCVPSTCYLRMNRYPPCPVSGQIFGLMPHTDSDFLTILHQDQVGGLELVK 252
             ++S FF ENCV +TCYLRMNRYPPCP   +++GLMPHTDSDFLTIL+QDQVGGL+L+K
Sbjct: 181 SGQNSSFFKENCVRNTCYLRMNRYPPCPKPSEVYGLMPHTDSDFLTILYQDQVGGLQLIK 240

Query: 253 DGKWIAVKPNPEALIINIGDLFQAWSNDVYKSVEHRVVTNSKVERFSIAYFLCPWSETVI 312
           D +WIAVKPNP+ALIINIGDLFQAWSN +YKSVEHRV+TN KVERFS AYF+CP  + VI
Sbjct: 241 DNRWIAVKPNPKALIINIGDLFQAWSNGMYKSVEHRVMTNPKVERFSTAYFMCPSYDAVI 300

Query: 313 KSKCEPGVYRRFSFREFRNQVQEDVRKHGYKIGLPRF 337
           +   +   YR FSFREFR QVQEDV+K G+K+GLPRF
Sbjct: 301 ECSSDRPAYRNFSFREFRQQVQEDVKKFGFKVGLPRF 333

BLAST of CaUC03G055000 vs. TAIR 10
Match: AT1G50960.1 (gibberellin 2-oxidase 7 )

HSP 1 Score: 295.4 bits (755), Expect = 5.7e-80
Identity = 150/337 (44.51%), Postives = 219/337 (64.99%), Query Frame = 0

Query: 11  VAMDPPFHEAYKALLG-----ETAAMAKGSDMVTVEEWELPVVDLSRLKAAE---AEECK 70
           +A  PPF   + ++ G      T+     +  +     +LPV+DLS L + E    + C 
Sbjct: 1   MASQPPFKTNFCSIFGSSFPNSTSESNTNTSTIQTSGIKLPVIDLSHLTSGEEVKRKRCV 60

Query: 71  NDIIKASKEWGFFQVVNHGISNQLLGKMRAKQIELFKQPFERKSKEDKFLNFSAGSYRWG 130
             ++ A+KEWGFFQ+VNHGI   +   M  ++ +LF QPF  K +E +F + S  SYRWG
Sbjct: 61  KQMVAAAKEWGFFQIVNHGIPKDVFEMMLLEEKKLFDQPFSVKVRE-RFSDLSKNSYRWG 120

Query: 131 TPSATSLTQLSWSEAFHVSLSDILG-SNGSDDLRSTMEEFAGKVSRLAQKLAEILGENLD 190
            PSATS  Q S SEAFH+ LS++   S+  ++LR+ +E +  +++R+AQ + EILG+ ++
Sbjct: 121 NPSATSPAQYSVSEAFHIILSEVSRISDDRNNLRTIVETYVQEIARVAQMICEILGKQVN 180

Query: 191 RSSKFFVENCVPSTCYLRMNRYPPCPVSGQIFGLMPHTDSDFLTILHQDQVGGLELVKDG 250
            SS++F         +LR+N+Y P     ++FGL+PHTD+ FLTIL QDQ+GGLEL  +G
Sbjct: 181 VSSEYFENIFELENSFLRLNKYHPSVFGSEVFGLVPHTDTSFLTILSQDQIGGLELENNG 240

Query: 251 KWIAVKPNPEALIINIGDLFQAWSNDVYKSVEHRVVTNSKVERFSIAYFLCPWSETVIKS 310
           +WI+VKP  EAL +NIGD+FQA SN VY+SV HRV++ + +ER SIA+F+CP+ ET I  
Sbjct: 241 QWISVKPCLEALTVNIGDMFQALSNGVYQSVRHRVISPANIERMSIAFFVCPYLETEIDC 300

Query: 311 KCEPGVYRRFSFREFRNQVQEDVRKHGYKIGLPRFAL 339
              P  YRRFSFRE++ Q + DV++ G K+GL RF +
Sbjct: 301 FGYPKKYRRFSFREYKEQSEHDVKETGDKVGLSRFLI 336

BLAST of CaUC03G055000 vs. TAIR 10
Match: AT5G58660.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 177.2 bits (448), Expect = 2.3e-44
Identity = 121/340 (35.59%), Postives = 173/340 (50.88%), Query Frame = 0

Query: 28  TAAMAKGSDMVTVEEWELPVVDLSRLKAAEAEECKNDIIKASKEWGFFQVVNHGISNQLL 87
           T  + +G D+       +PV+DL RL        K  + +A KEWG F++ NHG+   L 
Sbjct: 23  TPVIVQGKDI------NIPVIDLERLD-------KEILREACKEWGIFRLENHGVPLALT 82

Query: 88  GKMRAKQIELFKQPFERKSKEDKFLNFSAGSYRWGTPS----------ATSLTQLSWSEA 147
            +++     L   PFE+K +    +  S  SY WGTP+              + L+  E 
Sbjct: 83  SRLQEISESLLSLPFEKKRELFAAVK-SPLSYFWGTPALNRSGDALKRGAQASNLTMLEG 142

Query: 148 FHV------SLSDILGSNGSDD----------LRSTMEEFAGKVSRLAQKLAEILGE--N 207
           F+V      SLS +  S   DD           R  MEE+   ++R+A  L E + +  N
Sbjct: 143 FNVPLSSLSSLSKLPTSTCCDDDAQEEPKLESFRVLMEEYGKHITRIAVSLFEAIAQTLN 202

Query: 208 LDRSSKFFVENCVPSTCYLRMNRYPPC--PVSGQIFGLMPHTDSDFLTILHQDQVGGLEL 267
           L+ S     E    ST  +R+ RYP      + +  G+  HTDS  ++IL +D+ GGLE+
Sbjct: 203 LELSGNRRSEYLSESTGLIRVYRYPQSSEEAAREALGMEVHTDSSVISILREDESGGLEI 262

Query: 268 VKDGKWIAVKPNPEALIINIGDLFQAWSNDVYKSVEHRV-VTNSKVERFSIAYFLCPWSE 327
           +K  +W  VKP    LI+N+GD+ QA S+D YKSV HRV   N K ER S+ YF+ P  +
Sbjct: 263 MKGEEWFCVKPVANTLIVNLGDMMQAISDDEYKSVTHRVKKRNRKTERHSVCYFVFPKRD 322

Query: 328 TVIKSKCEPGVYRRFSFREFRNQVQEDVRKHGYKIGLPRF 337
            VIKS      Y+ F++ +F  QVQ DV+  G KIGLPRF
Sbjct: 323 CVIKS----SNYKLFTYSDFEAQVQADVQSLGTKIGLPRF 344

BLAST of CaUC03G055000 vs. TAIR 10
Match: AT5G07200.1 (gibberellin 20-oxidase 3 )

HSP 1 Score: 162.2 bits (409), Expect = 7.5e-40
Identity = 97/285 (34.04%), Postives = 141/285 (49.47%), Query Frame = 0

Query: 40  VEEWELPVVDLSRLKAAE---AEECKNDIIKASKEWGFFQVVNHGISNQLLGKMRAKQIE 99
           V+  ++P++DL+   + +   A E    + KA+ + GFF + NHG+   LL +       
Sbjct: 53  VQPLQVPLIDLAGFLSGDSCLASEATRLVSKAATKHGFFLITNHGVDESLLSRAYLHMDS 112

Query: 100 LFKQPFERKSKEDKFLNFSAGSYRWGTPS--ATSL-----TQLSWSEAFHVSLS------ 159
            FK P   K K  +         +WG  S  A+S      ++L W E      S      
Sbjct: 113 FFKAPACEKQKAQR---------KWGESSGYASSFVGRFSSKLPWKETLSFKFSPEEKIH 172

Query: 160 -----DILG---SNGSDDLRSTMEEFAGKVSRLAQKLAEILGENLDRSSKFFVENCVPST 219
                D +     +G +D     +E+A  ++ L+ K+ E+LG +L    ++F E    S 
Sbjct: 173 SQTVKDFVSKKMGDGYEDFGKVYQEYAEAMNTLSLKIMELLGMSLGVERRYFKEFFEDSD 232

Query: 220 CYLRMNRYPPCPVSGQIFGLMPHTDSDFLTILHQDQVGGLELVKDGKWIAVKPNPEALII 279
              R+N YP C       G  PH D   LTILHQDQVGGL++  D KW ++ PNP A ++
Sbjct: 233 SIFRLNYYPQCKQPELALGTGPHCDPTSLTILHQDQVGGLQVFVDNKWQSIPPNPHAFVV 292

Query: 280 NIGDLFQAWSNDVYKSVEHRVVTNSKVERFSIAYFLCPWSETVIK 301
           NIGD F A +N  YKS  HR V NS+ ER + A+FLCP  E V+K
Sbjct: 293 NIGDTFMALTNGRYKSCLHRAVVNSERERKTFAFFLCPKGEKVVK 328

BLAST of CaUC03G055000 vs. TAIR 10
Match: AT3G19000.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 159.8 bits (403), Expect = 3.7e-39
Identity = 105/306 (34.31%), Postives = 152/306 (49.67%), Query Frame = 0

Query: 44  ELPVVDLSRLKAAEAEE--CKNDIIKASKEWGFFQVVNHGISNQLLGKMRAKQIELFKQP 103
           E+P +DLS L+    ++     +I +A K WGFFQV+NHG+ + L  ++     E F   
Sbjct: 31  EIPTIDLSSLEDTHHDKTAIAKEIAEACKRWGFFQVINHGLPSALRHRVEKTAAEFFNLT 90

Query: 104 FERKSKEDKFLNFSAGSYRWGTPSATSLTQLSWSEAFHVSLSD---ILGSNGSDD----- 163
            E K K  +      G +        +     W E F   L D   +  S   +D     
Sbjct: 91  TEEKRKVKRDEVNPMGYH----DEEHTKNVRDWKEIFDFFLQDSTIVPASPEPEDTELRK 150

Query: 164 -----------LRSTMEEFAGKVSRLAQKLAEILGENL----DRSSKFFVENCVPSTCYL 223
                       R   +E+A +V +LA +L E++  +L    DR + FF E     T +L
Sbjct: 151 LTNQWPQNPSHFREVCQEYAREVEKLAFRLLELVSISLGLPGDRLTGFFNE----QTSFL 210

Query: 224 RMNRYPPCPVSGQIFGLMPHTDSDFLTILHQDQVGGLELVK--DGKWIAVKPNPEALIIN 283
           R N YPPCP      G+  H D   LT+L QD VGGL++ +  DG+WI VKP  +ALIIN
Sbjct: 211 RFNHYPPCPNPELALGVGRHKDGGALTVLAQDSVGGLQVSRRSDGQWIPVKPISDALIIN 270

Query: 284 IGDLFQAWSNDVYKSVEHRVVTNSKVERFSIAYFLCPWSETVIK------SKCEPGVYRR 317
           +G+  Q W+ND Y S EHRVV N+  ERFSI +F  P  E  I+      S+  P  Y++
Sbjct: 271 MGNCIQVWTNDEYWSAEHRVVVNTSKERFSIPFFFFPSHEANIEPLEELISEENPPCYKK 328

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038894099.11.4e-18190.91gibberellin 2-beta-dioxygenase 8 isoform X1 [Benincasa hispida][more]
XP_023520137.15.7e-17587.72gibberellin 2-beta-dioxygenase 8 [Cucurbita pepo subsp. pepo][more]
XP_023001285.14.9e-17487.13gibberellin 2-beta-dioxygenase 8 [Cucurbita maxima][more]
XP_022933193.11.4e-17387.13gibberellin 2-beta-dioxygenase 8 [Cucurbita moschata][more]
XP_008441451.14.6e-17288.41PREDICTED: gibberellin 2-beta-dioxygenase 8 isoform X2 [Cucumis melo][more]
Match NameE-valueIdentityDescription
O495611.3e-11661.72Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis thaliana OX=3702 GN=GA2OX8 PE=1 ... [more]
Q7XP651.2e-9048.86Gibberellin 2-beta-dioxygenase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2... [more]
Q8LGZ91.8e-8349.25Gibberellin 2-beta-dioxygenase 5 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2... [more]
Q9C6I48.0e-7944.51Gibberellin 2-beta-dioxygenase 7 OS=Arabidopsis thaliana OX=3702 GN=GA2OX7 PE=1 ... [more]
Q391121.1e-3834.04Gibberellin 20 oxidase 3 OS=Arabidopsis thaliana OX=3702 GN=GA20OX3 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1KKS12.4e-17487.13gibberellin 2-beta-dioxygenase 8 OS=Cucurbita maxima OX=3661 GN=LOC111495461 PE=... [more]
A0A6J1F4226.8e-17487.13gibberellin 2-beta-dioxygenase 8 OS=Cucurbita moschata OX=3662 GN=LOC111440045 P... [more]
A0A1S3B3092.2e-17288.41gibberellin 2-beta-dioxygenase 8 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103485... [more]
A0A1S3B3F31.4e-17189.32gibberellin 2-beta-dioxygenase 8 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103485... [more]
A0A5A7UM122.3e-16989.46Gibberellin 2-beta-dioxygenase 8 isoform X1 OS=Cucumis melo var. makuwa OX=11946... [more]
Match NameE-valueIdentityDescription
AT4G21200.19.0e-11861.72gibberellin 2-oxidase 8 [more]
AT1G50960.15.7e-8044.51gibberellin 2-oxidase 7 [more]
AT5G58660.12.3e-4435.592-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT5G07200.17.5e-4034.04gibberellin 20-oxidase 3 [more]
AT3G19000.13.7e-3934.312-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (USVL246-FR2) v1
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR044861Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domainPFAMPF031712OG-FeII_Oxycoord: 199..293
e-value: 2.1E-28
score: 98.7
IPR027443Isopenicillin N synthase-like superfamilyGENE3D2.60.120.330coord: 43..331
e-value: 1.2E-79
score: 269.9
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 45..142
e-value: 2.5E-16
score: 60.5
NoneNo IPR availablePANTHERPTHR479902-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATEDcoord: 31..331
NoneNo IPR availablePANTHERPTHR47990:SF13GIBBERELLIN 2-BETA-DIOXYGENASE 8-LIKEcoord: 31..331
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 38..322
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 194..293
score: 14.603428

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CaUC03G055000.1CaUC03G055000.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0052635 C-20 gibberellin 2-beta-dioxygenase activity
molecular_function GO:0046872 metal ion binding