CaUC03G053360 (gene) Watermelon (USVL246-FR2) v1

Overview
NameCaUC03G053360
Typegene
OrganismCitrullus amarus (Watermelon (USVL246-FR2) v1)
DescriptionPectinesterase
LocationCiama_Chr03: 3948382 .. 3949948 (-)
RNA-Seq ExpressionCaUC03G053360
SyntenyCaUC03G053360
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCTTTTTCCAGCTTGTTGCATGTTGTGTTATTATCTGTTTCTTCAATTCCCTAGTACTAATTGATTCCATCAATTTTAATGCAACGGTGTCGTTAAATGGATCCGGAGATTTCAAAAGCATTAATGATGCAATTGCAGCCGCTCCAAACCATTCTAATACAAGATTCTATATTCATGTCAGCCCCGGCAGTTATAATGAACGTCTTGAAATTCCATCTGCCAAGAAGTTCATTGCTTTGATCGGGGATAATGCGTTCACAACCATTATTGTCGGTAATCGAAGCAATGGGACAGGATTCAAGACCAATAACTCCGCAACTCTCAGTAAGCTTTTCCTTTCTAATTTTTTCTGTTATGGTCTCTCTCTTATCCCTCATTATTCACACACACACGTTTAATTTGAAGATCTTAAGGTTTCAGTCTTGTTTTTGTTAACTTGATTTTTTATTCATTTTGCTTGTTTTTAACTTTCAAATGTTGTGTGTGTTAAGAATTTTGGGCTTAAATTAATGTATAAGAACACTGTGTAACAGAAACATATGAAAACAATAGTTAGGTATGTCCTTTATTAGTTAACTAAATGAGTAAATGTTTGTATGATGAACAAGTGTGAATTTTGAAGTTAGTAAATGTTGAAGCCTCGGATTTTAAAATAACCCATCTTCTTTTCGGAGACAGATGGTAAAGTTGTATTTTTTTTTTTTTTTGGTTAAAGGTAATTATATATATATATCATCCAGAGAGAAATCAATTTTACCTGTGAAATGGGTTAAAAGGCGTATTAAAGAAATTATTTACCGTTTTAATAAGGTTTTGGTGTAAGGAATTGCATGAATTTTAATCTTTTACTAATTCTGTGTATTTTTATTTGATGAAGCCGTGAAAGGTGACAATTTTATGGCACAACATTTGACCTTTGAAAATTCTGCGGGACCTCACAACGGGCAAGCAGTGGCAGTACTTGACCAGGCCGACCACACTGCTTATTACAAGTGTAGATTTATGGGTTTCCAAGACACTCTCTACGTAAAAGGACATAGACAGTTTTTCAAGGAGTGTGAGGTTTATGGAAGTGTTGACTTTATTTTTGGGAGTGGACTTGTTTTGTTCCAAGACACTAACATCTATGCTCGGCTACAAAGTGTTGATGTCACAGTCACCGCACAGTCAAAAGAACGTTCAATGTCTTCAAGTGGCTTCTCCTTTCAGAACTGTACGGTTATGGTTTCGCCAGAGATAAAAGCCAATAAGTCCAATGTTAAGATCTACTTAGGTCGACCATGGAAACAATATTCAAGTGTTGTGTTCATGGAGTCATTTCTTGATGATGTGGTGGACCCAAAGGGATGGATGATTTGGGAAGGAGTACCAGTAAACAACTTATTTTATGCCGAGTTTAATAATCGAGGTCCTGGTGCTGACGTGGCCAACCGAGTTAATTGGCCTGGTTTTCATGTAATAGACAAAAAATCAGCAAAAAAATTTACCATCGAATACTTTATTAACGGGACTTATTGGTTGCCCGAAACAGGTGTACCCTTTAGACTTGGATTGCTTTCCTAA

mRNA sequence

ATGGCCTTTTTCCAGCTTGTTGCATGTTGTGTTATTATCTGTTTCTTCAATTCCCTAGTACTAATTGATTCCATCAATTTTAATGCAACGGTGTCGTTAAATGGATCCGGAGATTTCAAAAGCATTAATGATGCAATTGCAGCCGCTCCAAACCATTCTAATACAAGATTCTATATTCATGTCAGCCCCGGCAGTTATAATGAACGTCTTGAAATTCCATCTGCCAAGAAGTTCATTGCTTTGATCGGGGATAATGCGTTCACAACCATTATTGTCGGTAATCGAAGCAATGGGACAGGATTCAAGACCAATAACTCCGCAACTCTCACCGTGAAAGGTGACAATTTTATGGCACAACATTTGACCTTTGAAAATTCTGCGGGACCTCACAACGGGCAAGCAGTGGCAGTACTTGACCAGGCCGACCACACTGCTTATTACAAGTGTAGATTTATGGGTTTCCAAGACACTCTCTACGTAAAAGGACATAGACAGTTTTTCAAGGAGTGTGAGGTTTATGGAAGTGTTGACTTTATTTTTGGGAGTGGACTTGTTTTGTTCCAAGACACTAACATCTATGCTCGGCTACAAAGTGTTGATGTCACAGTCACCGCACAGTCAAAAGAACGTTCAATGTCTTCAAGTGGCTTCTCCTTTCAGAACTGTACGGTTATGGTTTCGCCAGAGATAAAAGCCAATAAGTCCAATGTTAAGATCTACTTAGGTCGACCATGGAAACAATATTCAAGTGTTGTGTTCATGGAGTCATTTCTTGATGATGTGGTGGACCCAAAGGGATGGATGATTTGGGAAGGAGTACCAGTAAACAACTTATTTTATGCCGAGTTTAATAATCGAGGTCCTGGTGCTGACGTGGCCAACCGAGTTAATTGGCCTGGTTTTCATGTAATAGACAAAAAATCAGCAAAAAAATTTACCATCGAATACTTTATTAACGGGACTTATTGGTTGCCCGAAACAGGTGTACCCTTTAGACTTGGATTGCTTTCCTAA

Coding sequence (CDS)

ATGGCCTTTTTCCAGCTTGTTGCATGTTGTGTTATTATCTGTTTCTTCAATTCCCTAGTACTAATTGATTCCATCAATTTTAATGCAACGGTGTCGTTAAATGGATCCGGAGATTTCAAAAGCATTAATGATGCAATTGCAGCCGCTCCAAACCATTCTAATACAAGATTCTATATTCATGTCAGCCCCGGCAGTTATAATGAACGTCTTGAAATTCCATCTGCCAAGAAGTTCATTGCTTTGATCGGGGATAATGCGTTCACAACCATTATTGTCGGTAATCGAAGCAATGGGACAGGATTCAAGACCAATAACTCCGCAACTCTCACCGTGAAAGGTGACAATTTTATGGCACAACATTTGACCTTTGAAAATTCTGCGGGACCTCACAACGGGCAAGCAGTGGCAGTACTTGACCAGGCCGACCACACTGCTTATTACAAGTGTAGATTTATGGGTTTCCAAGACACTCTCTACGTAAAAGGACATAGACAGTTTTTCAAGGAGTGTGAGGTTTATGGAAGTGTTGACTTTATTTTTGGGAGTGGACTTGTTTTGTTCCAAGACACTAACATCTATGCTCGGCTACAAAGTGTTGATGTCACAGTCACCGCACAGTCAAAAGAACGTTCAATGTCTTCAAGTGGCTTCTCCTTTCAGAACTGTACGGTTATGGTTTCGCCAGAGATAAAAGCCAATAAGTCCAATGTTAAGATCTACTTAGGTCGACCATGGAAACAATATTCAAGTGTTGTGTTCATGGAGTCATTTCTTGATGATGTGGTGGACCCAAAGGGATGGATGATTTGGGAAGGAGTACCAGTAAACAACTTATTTTATGCCGAGTTTAATAATCGAGGTCCTGGTGCTGACGTGGCCAACCGAGTTAATTGGCCTGGTTTTCATGTAATAGACAAAAAATCAGCAAAAAAATTTACCATCGAATACTTTATTAACGGGACTTATTGGTTGCCCGAAACAGGTGTACCCTTTAGACTTGGATTGCTTTCCTAA

Protein sequence

MAFFQLVACCVIICFFNSLVLIDSINFNATVSLNGSGDFKSINDAIAAAPNHSNTRFYIHVSPGSYNERLEIPSAKKFIALIGDNAFTTIIVGNRSNGTGFKTNNSATLTVKGDNFMAQHLTFENSAGPHNGQAVAVLDQADHTAYYKCRFMGFQDTLYVKGHRQFFKECEVYGSVDFIFGSGLVLFQDTNIYARLQSVDVTVTAQSKERSMSSSGFSFQNCTVMVSPEIKANKSNVKIYLGRPWKQYSSVVFMESFLDDVVDPKGWMIWEGVPVNNLFYAEFNNRGPGADVANRVNWPGFHVIDKKSAKKFTIEYFINGTYWLPETGVPFRLGLLS
Homology
BLAST of CaUC03G053360 vs. NCBI nr
Match: XP_023511664.1 (probable pectinesterase/pectinesterase inhibitor 7 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 510.8 bits (1314), Expect = 9.2e-141
Identity = 249/335 (74.33%), Postives = 281/335 (83.88%), Query Frame = 0

Query: 1   MAFFQLVACCVIICFFNSLVLIDSINFNATVSLNGSGDFKSINDAIAAAPNHSNTRFYIH 60
           MAF QLVA C+   F N L L+D I FNATVSL+GSGDFKSI++AIAAAPN+SNTRFYI 
Sbjct: 1   MAFLQLVA-CLFFFFANVLALVDPIRFNATVSLDGSGDFKSISEAIAAAPNNSNTRFYIR 60

Query: 61  VSPGSYNERLEIPSAKKFIALIGDNAFTTIIVGNRSNGTGFKTNNSATLTVKGDNFMAQH 120
           V+PG Y   LEIP AKKFIALIGDNA TTIIV NRS+GTGFKT+ S TL V GDNFMAQH
Sbjct: 61  VTPGIYRGHLEIPPAKKFIALIGDNALTTIIVDNRSHGTGFKTSESTTLVVNGDNFMAQH 120

Query: 121 LTFENSAGPHNGQAVAVLDQADHTAYYKCRFMGFQDTLYVKGHRQFFKECEVYGSVDFIF 180
           L+FENSA P NGQAVAVLD A HT YY C+F+ FQDTLY KG  QFF+EC++YG+VDFIF
Sbjct: 121 LSFENSAEPQNGQAVAVLDYAKHTVYYDCKFLSFQDTLYSKGEFQFFRECDIYGTVDFIF 180

Query: 181 GSGLVLFQDTNIYARLQSVDVTVTAQSKERSMSSSGFSFQNCTVMVSPEIKANKSNVKIY 240
           G+GL +FQD NIYARL   ++TVTAQSKE+SMS SGFSFQNCTV VSPEI ANK+NVKIY
Sbjct: 181 GNGLAVFQDCNIYARLPMDEITVTAQSKEQSMSQSGFSFQNCTVTVSPEITANKTNVKIY 240

Query: 241 LGRPWKQYSSVVFMESFLDDVVDPKGWMIWEGVPVNNLFYAEFNNRGPGADVANRVNWPG 300
           LGRPW++YS+VVFMESFLDD V P+GW+ W GVP+NNLFYAEFNNRGPGAD  +RVNWP 
Sbjct: 241 LGRPWREYSTVVFMESFLDDNVQPQGWISWGGVPINNLFYAEFNNRGPGADTTHRVNWPT 300

Query: 301 FHVIDKKSAKKFTIEYFINGTYWLPETGVPFRLGL 336
           FHV+DK  AK FT+E FING+ WLPET VPFR GL
Sbjct: 301 FHVLDKLLAKNFTVENFINGSDWLPETHVPFRTGL 334

BLAST of CaUC03G053360 vs. NCBI nr
Match: KAG6572387.1 (putative pectinesterase/pectinesterase inhibitor 7, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 510.4 bits (1313), Expect = 1.2e-140
Identity = 249/335 (74.33%), Postives = 281/335 (83.88%), Query Frame = 0

Query: 1   MAFFQLVACCVIICFFNSLVLIDSINFNATVSLNGSGDFKSINDAIAAAPNHSNTRFYIH 60
           MAF QL A C+   F N L L+DSI FNA VSL+GSGDFKSI++AIAAAPN+SNTRFYI 
Sbjct: 1   MAFLQL-AACLFFFFANVLALVDSIRFNAVVSLDGSGDFKSISEAIAAAPNNSNTRFYIR 60

Query: 61  VSPGSYNERLEIPSAKKFIALIGDNAFTTIIVGNRSNGTGFKTNNSATLTVKGDNFMAQH 120
           V+PG Y   LEIP AKKFIALIGDNA TTIIV NRS+GTGFKT+ SATL V GDNFMAQH
Sbjct: 61  VTPGIYRGHLEIPPAKKFIALIGDNALTTIIVDNRSHGTGFKTSESATLVVNGDNFMAQH 120

Query: 121 LTFENSAGPHNGQAVAVLDQADHTAYYKCRFMGFQDTLYVKGHRQFFKECEVYGSVDFIF 180
           L+FENSA P NGQAVAVLD A HT YY CRFM FQDTLY KG  QFF+EC++YG+VDFIF
Sbjct: 121 LSFENSAEPQNGQAVAVLDYAKHTVYYNCRFMSFQDTLYSKGEFQFFRECDIYGTVDFIF 180

Query: 181 GSGLVLFQDTNIYARLQSVDVTVTAQSKERSMSSSGFSFQNCTVMVSPEIKANKSNVKIY 240
           G+GL +FQD NIYARL   ++TVTAQSKE+S+S SGFSFQNCTV VSPEI ANK+ VKIY
Sbjct: 181 GNGLAVFQDCNIYARLPMDEITVTAQSKEQSVSQSGFSFQNCTVTVSPEITANKTKVKIY 240

Query: 241 LGRPWKQYSSVVFMESFLDDVVDPKGWMIWEGVPVNNLFYAEFNNRGPGADVANRVNWPG 300
           LGRPW++YS+VVFMESFLDD V P+GW+ WEGVP+NNLFYAEFNNRGPGA+  +RVNWP 
Sbjct: 241 LGRPWREYSTVVFMESFLDDNVQPQGWISWEGVPINNLFYAEFNNRGPGANTTHRVNWPT 300

Query: 301 FHVIDKKSAKKFTIEYFINGTYWLPETGVPFRLGL 336
           FHV+DK  AK FT+E FING+ WLPET VPFR GL
Sbjct: 301 FHVLDKLLAKNFTVENFINGSDWLPETHVPFRTGL 334

BLAST of CaUC03G053360 vs. NCBI nr
Match: XP_022952993.1 (probable pectinesterase/pectinesterase inhibitor 7 [Cucurbita moschata])

HSP 1 Score: 506.5 bits (1303), Expect = 1.7e-139
Identity = 247/335 (73.73%), Postives = 281/335 (83.88%), Query Frame = 0

Query: 1   MAFFQLVACCVIICFFNSLVLIDSINFNATVSLNGSGDFKSINDAIAAAPNHSNTRFYIH 60
           MAF QL A C+   F N L L+DSI FNA VSL+GSGDFKSI++AIAAAPN+SNTRFYI 
Sbjct: 1   MAFLQL-AACLFFFFANVLALVDSIRFNAVVSLDGSGDFKSISEAIAAAPNNSNTRFYIR 60

Query: 61  VSPGSYNERLEIPSAKKFIALIGDNAFTTIIVGNRSNGTGFKTNNSATLTVKGDNFMAQH 120
           V+PG Y   LEIP AKKFIALIGD+A TTIIV NRS+GTGFKT+ SATL V GDNFMAQH
Sbjct: 61  VTPGIYRGHLEIPPAKKFIALIGDSALTTIIVDNRSHGTGFKTSESATLVVNGDNFMAQH 120

Query: 121 LTFENSAGPHNGQAVAVLDQADHTAYYKCRFMGFQDTLYVKGHRQFFKECEVYGSVDFIF 180
           L+FENSA P NGQAVAVLD A H+ YY CRFM FQDTLY KG  QFF+EC++YG+VDFIF
Sbjct: 121 LSFENSAEPQNGQAVAVLDYAKHSVYYDCRFMSFQDTLYSKGEFQFFRECDIYGTVDFIF 180

Query: 181 GSGLVLFQDTNIYARLQSVDVTVTAQSKERSMSSSGFSFQNCTVMVSPEIKANKSNVKIY 240
           G+GL +FQD NIYARL   ++TVTAQSKE+S+S SGFSFQNCTV VSPEI ANK+ VKIY
Sbjct: 181 GNGLAVFQDCNIYARLPMDEITVTAQSKEQSVSQSGFSFQNCTVTVSPEITANKTKVKIY 240

Query: 241 LGRPWKQYSSVVFMESFLDDVVDPKGWMIWEGVPVNNLFYAEFNNRGPGADVANRVNWPG 300
           LGRPW++YS+VVFMESFLDD V P+GW+ WEGVP+NNLFYAEFNNRGPGA+  +RVNWP 
Sbjct: 241 LGRPWREYSTVVFMESFLDDNVQPQGWISWEGVPINNLFYAEFNNRGPGANTTHRVNWPT 300

Query: 301 FHVIDKKSAKKFTIEYFINGTYWLPETGVPFRLGL 336
           FHV+DK  AK FT+E FING+ WLPET VPFR GL
Sbjct: 301 FHVLDKLLAKNFTVENFINGSDWLPETHVPFRTGL 334

BLAST of CaUC03G053360 vs. NCBI nr
Match: KAA0055286.1 (putative pectinesterase/pectinesterase inhibitor 47 [Cucumis melo var. makuwa])

HSP 1 Score: 500.0 bits (1286), Expect = 1.6e-137
Identity = 236/319 (73.98%), Postives = 277/319 (86.83%), Query Frame = 0

Query: 19  LVLIDSINFNATVSLNGSGDFKSINDAIAAAPNHSNTRFYIHVSPGSYNERLEIPSAKKF 78
           ++ + SI+FN TVSL+GSG+FK I+DAI AAPN+SNTRFYIHV+PG+Y+E ++IP AKKF
Sbjct: 1   MLFVHSISFNLTVSLDGSGNFKRISDAIVAAPNNSNTRFYIHVAPGTYHEAIQIPKAKKF 60

Query: 79  IALIGDNAFTTIIVGNRSNGTGFKTNNSATLTVKGDNFMAQHLTFENSAGPHNGQAVAVL 138
           IALIGD+AFTTII+ NRSNGTGFKTNNSATLTV+GDNFMA+ LTFENSAGP NGQAVAV 
Sbjct: 61  IALIGDDAFTTIIIDNRSNGTGFKTNNSATLTVQGDNFMAKSLTFENSAGPQNGQAVAVF 120

Query: 139 DQADHTAYYKCRFMGFQDTLYVKGHRQFFKECEVYGSVDFIFGSGLVLFQDTNIYARLQS 198
           D+ADHTAYYKCRF+GFQDTLYV G  QFFKE  +YGSVDFIFG G V+FQD NIYAR+ S
Sbjct: 121 DKADHTAYYKCRFLGFQDTLYVNGKYQFFKESNIYGSVDFIFGHGRVMFQDCNIYARMPS 180

Query: 199 VDVTVTAQSKERSMSSSGFSFQNCTVMVSPEIKANKSNVKIYLGRPWKQYSSVVFMESFL 258
             +TVTAQSK+   S SGFSFQNCTV VSPEI +NK NVK++LGRPW+QYS+VVFMESFL
Sbjct: 181 NSITVTAQSKDFLRSISGFSFQNCTVTVSPEISSNKQNVKVFLGRPWRQYSNVVFMESFL 240

Query: 259 DDVVDPKGWMIWEGVPVNNLFYAEFNNRGPGADVANRVNWPGFHVIDKKSAKKFTIEYFI 318
           DDVV  +GWM W+GVPVNNLFY EFNN GPGADV+ RVNW G+H++DKKSA  FT++ FI
Sbjct: 241 DDVVASEGWMEWKGVPVNNLFYGEFNNFGPGADVSKRVNWTGYHLLDKKSAIGFTVDNFI 300

Query: 319 NGTYWLPETGVPFRLGLLS 338
           +G+ WL ETG+PFR GLL+
Sbjct: 301 DGSEWLAETGIPFRRGLLT 319

BLAST of CaUC03G053360 vs. NCBI nr
Match: XP_022952377.1 (probable pectinesterase/pectinesterase inhibitor 7 isoform X1 [Cucurbita moschata])

HSP 1 Score: 495.0 bits (1273), Expect = 5.2e-136
Identity = 240/337 (71.22%), Postives = 281/337 (83.38%), Query Frame = 0

Query: 1   MAFFQLVACCVIICFFNSLVLIDSINFNATVSLNGSGDFKSINDAIAAAPNHSNTRFYIH 60
           MAFFQ ++ C  +CF N L ++DS +FNATV+L+G+G+FKSI DAIAAAPN+SNTRFYI 
Sbjct: 1   MAFFQRIS-CFFLCFLNFLAVVDSTHFNATVALDGTGNFKSITDAIAAAPNNSNTRFYIR 60

Query: 61  VSPGSYNERLEIPSAKKFIALIGDNAFTTIIVGNRSNGTGFKTNNSATLTVKGDNFMAQH 120
           VSPG Y+E LEI SAKKFIALIG+N FTTIIV NRSN  GF+T+ SAT+ VKG+NFMAQH
Sbjct: 61  VSPGIYHEHLEIASAKKFIALIGENEFTTIIVDNRSNAGGFRTSESATMVVKGNNFMAQH 120

Query: 121 LTFENSAGPHNGQAVAVLDQADHTAYYKCRFMGFQDTLYVKGHRQFFKECEVYGSVDFIF 180
           L+FENSA   +GQAVAVLD A +T YYKCRF+ FQDTLY KG  QFFK C++YG+VDFIF
Sbjct: 121 LSFENSADHQDGQAVAVLDDAKYTVYYKCRFLSFQDTLYSKGEYQFFKGCDIYGTVDFIF 180

Query: 181 GSGLVLFQDTNIYARLQSVDVTVTAQSKERSMSSSGFSFQNCTVMVSPEIKANKSNVKIY 240
           G+GL +FQD N+YARL S D TVTAQSK +  + SGFSFQNCTV VSPEI + K+ VKIY
Sbjct: 181 GNGLAMFQDCNVYARLPSNDSTVTAQSK-KQWTQSGFSFQNCTVTVSPEIASCKALVKIY 240

Query: 241 LGRPWKQYSSVVFMESFLDDVVDPKGWMIWEGVPVNNLFYAEFNNRGPGADVANRVNWPG 300
           LGRPWK YS+VVFMESFLDD V P+GWM+W GVPVNNLFYAE+NNRGPGA+  +RVNWPG
Sbjct: 241 LGRPWKPYSTVVFMESFLDDNVQPQGWMLWPGVPVNNLFYAEYNNRGPGANTTHRVNWPG 300

Query: 301 FHVIDKKSAKKFTIEYFINGTYWLPETGVPFRLGLLS 338
           FHVID++ AK FT+E FINGTYWLPET VP+  GL S
Sbjct: 301 FHVIDRQLAKSFTVENFINGTYWLPETNVPYTSGLYS 335

BLAST of CaUC03G053360 vs. ExPASy Swiss-Prot
Match: O81415 (Probable pectinesterase/pectinesterase inhibitor 39 OS=Arabidopsis thaliana OX=3702 GN=PME39 PE=2 SV=1)

HSP 1 Score: 301.6 bits (771), Expect = 1.1e-80
Identity = 142/319 (44.51%), Postives = 214/319 (67.08%), Query Frame = 0

Query: 22  IDSINFNATVSLNGSGDFKSINDAIAAAPNHSNTRFYIHVSPGSYNERLEIPSAKKFIAL 81
           +   N+N +V+++G+G+F +INDA+ AAPN S TRF I++  G Y E +E+P  K  I  
Sbjct: 215 VQETNYNLSVAIDGTGNFTTINDAVFAAPNMSETRFIIYIKGGEYFENVELPKKKTMIMF 274

Query: 82  IGDNAFTTIIVGNRSNGTGFKTNNSATLTVKGDNFMAQHLTFENSAGPHNGQAVAVLDQA 141
           IGD    T+I  NRS   G+ T  + T+ VKG  ++A+ ++F NSAGP   QAVA    +
Sbjct: 275 IGDGIGKTVIKANRSRIDGWSTFQTPTVGVKGKGYIAKDISFVNSAGPAKAQAVAFRSGS 334

Query: 142 DHTAYYKCRFMGFQDTLYVKGHRQFFKECEVYGSVDFIFGSGLVLFQDTNIYARLQSV-- 201
           DH+A+Y+C F G+QDTLYV   +QF++EC++YG++DFIFG+  V+FQ++++YAR  +   
Sbjct: 335 DHSAFYRCEFDGYQDTLYVHSAKQFYRECDIYGTIDFIFGNAAVVFQNSSLYARKPNPGH 394

Query: 202 DVTVTAQSKERSMSSSGFSFQNCTVMVSPEIKANKSNVKIYLGRPWKQYSSVVFMESFLD 261
            +  TAQS+ +S   +G S  NC ++ +P++   K N K YLGRPW++YS  V ++SF+D
Sbjct: 395 KIAFTAQSRNQSDQPTGISILNCRILAAPDLIPVKENFKAYLGRPWRKYSRTVIIKSFID 454

Query: 262 DVVDPKGWMIWEG---VPVNNLFYAEFNNRGPGADVANRVNWPGFHVIDKKS-AKKFTIE 321
           D++ P GW+  EG     +  L+Y E+ N GPGA++A RV WPGF  I+ ++ A +FT+ 
Sbjct: 455 DLIHPAGWL--EGKKDFALETLYYGEYMNEGPGANMAKRVTWPGFRRIENQTEATQFTVG 514

Query: 322 YFINGTYWLPETGVPFRLG 335
            FI+G+ WL  TG+PF LG
Sbjct: 515 PFIDGSTWLNSTGIPFSLG 531

BLAST of CaUC03G053360 vs. ExPASy Swiss-Prot
Match: O04887 (Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 291.6 bits (745), Expect = 1.2e-77
Identity = 143/311 (45.98%), Postives = 205/311 (65.92%), Query Frame = 0

Query: 28  NATVSLNGSGDFKSINDAIAAAPNHSNTRFYIHVSPGSYNERLEIPSAKKFIALIGDNAF 87
           N  V+ +GSG+ K+I +A+AAA     +R+ I++  G+YNE +E+    K I  +GD   
Sbjct: 202 NIVVAQDGSGNVKTIQEAVAAASRAGGSRYVIYIKAGTYNENIEV--KLKNIMFVGDGIG 261

Query: 88  TTIIVGNRSNGTGFKTNNSATLTVKGDNFMAQHLTFENSAGPHNGQAVAVLDQADHTAYY 147
            TII G++S G G  T  SAT+ V GDNF+A+ +T  N+AGP+N QAVA+   +D + +Y
Sbjct: 262 KTIITGSKSVGGGATTFKSATVAVVGDNFIARDITIRNTAGPNNHQAVALRSGSDLSVFY 321

Query: 148 KCRFMGFQDTLYVKGHRQFFKECEVYGSVDFIFGSGLVLFQDTNIYARL-QSVDVTVTAQ 207
           +C F G+QDTLYV   RQF++EC++YG+VDFIFG+  V+ Q+ NI+AR   +   T+TAQ
Sbjct: 322 RCSFEGYQDTLYVHSQRQFYRECDIYGTVDFIFGNAAVVLQNCNIFARKPPNRTNTLTAQ 381

Query: 208 SKERSMSSSGFSFQNCTVMVSPEIKANKSNVKIYLGRPWKQYSSVVFMESFLDDVVDPKG 267
            +     S+G    NC V  + ++K  +S+VK +LGRPWKQYS  V++++FLD +++P G
Sbjct: 382 GRTDPNQSTGIIIHNCRVTAASDLKPVQSSVKTFLGRPWKQYSRTVYIKTFLDSLINPAG 441

Query: 268 WMIWEG-VPVNNLFYAEFNNRGPGADVANRVNWPGFHVIDKKS-AKKFTIEYFINGTYWL 327
           WM W G   +N L+YAE+ N GPG+  ANRV W G+HV+   S   +FT+  FI G  WL
Sbjct: 442 WMEWSGDFALNTLYYAEYMNTGPGSSTANRVKWRGYHVLTSPSQVSQFTVGNFIAGNSWL 501

Query: 328 PETGVPFRLGL 336
           P T VPF  GL
Sbjct: 502 PATNVPFTSGL 510

BLAST of CaUC03G053360 vs. ExPASy Swiss-Prot
Match: Q8RXK7 (Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana OX=3702 GN=PME41 PE=2 SV=2)

HSP 1 Score: 291.6 bits (745), Expect = 1.2e-77
Identity = 141/324 (43.52%), Postives = 210/324 (64.81%), Query Frame = 0

Query: 20  VLIDSINFNATVSLNGSGDFKSINDAIAAAPNHSNTR---FYIHVSPGSYNERLEIPSAK 79
           VL+  I    TV+ NG+G+F +I +A+ +APN ++     F I+V+ G Y E + I   K
Sbjct: 253 VLVSDI---VTVNQNGTGNFTTITEAVNSAPNKTDGTAGYFVIYVTSGVYEENVVIAKNK 312

Query: 80  KFIALIGDNAFTTIIVGNRSNGTGFKTNNSATLTVKGDNFMAQHLTFENSAGPHNGQAVA 139
           +++ +IGD    T++ GNR+   G+ T NSAT  V   NF+A ++TF N+AGP   QAVA
Sbjct: 313 RYLMMIGDGINRTVVTGNRNVVDGWTTFNSATFAVTSPNFVAVNMTFRNTAGPEKHQAVA 372

Query: 140 VLDQADHTAYYKCRFMGFQDTLYVKGHRQFFKECEVYGSVDFIFGSGLVLFQDTNIYAR- 199
           +   AD + +Y C F  +QDTLY    RQF++EC++YG+VDFIFG+  V+FQD N+Y R 
Sbjct: 373 MRSSADLSIFYSCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQDCNLYPRQ 432

Query: 200 -LQSVDVTVTAQSKERSMSSSGFSFQNCTVMVSPEIKANKSNVKIYLGRPWKQYSSVVFM 259
            +Q+    +TAQ +     ++G S  NCT+  + ++ ++   VK YLGRPWK+YS  VFM
Sbjct: 433 PMQNQFNAITAQGRTDPNQNTGISIHNCTIKPADDLVSSNYTVKTYLGRPWKEYSRTVFM 492

Query: 260 ESFLDDVVDPKGWMIWEG-VPVNNLFYAEFNNRGPGADVANRVNWPGFHVIDKKSAKKFT 319
           +S++D+VV+P GW  W G   ++ L+YAE+NN G G+   +RV WPG+HVI+   A  FT
Sbjct: 493 QSYIDEVVEPVGWREWNGDFALSTLYYAEYNNTGSGSSTTDRVVWPGYHVINSTDANNFT 552

Query: 320 IEYFINGTYWLPETGVPFRLGLLS 338
           +E F+ G  W+ ++GVP+  GLLS
Sbjct: 553 VENFLLGDGWMVQSGVPYISGLLS 573

BLAST of CaUC03G053360 vs. ExPASy Swiss-Prot
Match: O81301 (Probable pectinesterase/pectinesterase inhibitor 40 OS=Arabidopsis thaliana OX=3702 GN=PME40 PE=2 SV=1)

HSP 1 Score: 291.2 bits (744), Expect = 1.5e-77
Identity = 139/318 (43.71%), Postives = 206/318 (64.78%), Query Frame = 0

Query: 22  IDSINFNATVSLNGSGDFKSINDAIAAAPNHSNTRFYIHVSPGSYNERLEIPSAKKFIAL 81
           +D    N  V+ NG+G++ +I +AI+AAPN S TRF I++  G Y E +EIP  K  I  
Sbjct: 201 VDETKVNLVVAQNGTGNYTTIGEAISAAPNSSETRFVIYIKCGEYFENIEIPREKTMIMF 260

Query: 82  IGDNAFTTIIVGNRSNGTGFKTNNSATLTVKGDNFMAQHLTFENSAGPHNGQAVAVLDQA 141
           IGD    T+I  NRS   G+   +SAT+ V+G  F+A+ L+F N AGP   QAVA+   +
Sbjct: 261 IGDGIGRTVIKANRSYADGWTAFHSATVGVRGSGFIAKDLSFVNYAGPEKHQAVALRSSS 320

Query: 142 DHTAYYKCRFMGFQDTLYVKGHRQFFKECEVYGSVDFIFGSGLVLFQDTNIYARLQSVD- 201
           D +AYY+C F  +QDT+YV  H+QF++EC++YG+VDFIFG   V+FQ+ ++YAR  + + 
Sbjct: 321 DLSAYYRCSFESYQDTIYVHSHKQFYRECDIYGTVDFIFGDASVVFQNCSLYARRPNPNQ 380

Query: 202 -VTVTAQSKERSMSSSGFSFQNCTVMVSPEIKANKSNVKIYLGRPWKQYSSVVFMESFLD 261
            +  TAQ +E S   +G S  +  ++ +P++   ++N K YLGRPW+ YS  V M+SF+D
Sbjct: 381 KIIYTAQGRENSREPTGISIISSRILAAPDLIPVQANFKAYLGRPWQLYSRTVIMKSFID 440

Query: 262 DVVDPKGWMIW-EGVPVNNLFYAEFNNRGPGADVANRVNWPGFHVIDK-KSAKKFTIEYF 321
           D+VDP GW+ W +   +  L+Y E+ N GPG+++ NRV WPGF  I+  + A +F++  F
Sbjct: 441 DLVDPAGWLKWKDDFALETLYYGEYMNEGPGSNMTNRVQWPGFKRIETVEEASQFSVGPF 500

Query: 322 INGTYWLPETGVPFRLGL 336
           I+G  WL  T +PF L L
Sbjct: 501 IDGNKWLNSTRIPFTLDL 518

BLAST of CaUC03G053360 vs. ExPASy Swiss-Prot
Match: O22256 (Probable pectinesterase/pectinesterase inhibitor 20 OS=Arabidopsis thaliana OX=3702 GN=PME20 PE=2 SV=2)

HSP 1 Score: 290.8 bits (743), Expect = 2.0e-77
Identity = 140/314 (44.59%), Postives = 200/314 (63.69%), Query Frame = 0

Query: 30  TVSLNGSGDFKSINDAIAAAPNH---SNTRFYIHVSPGSYNERLEIPSAKKFIALIGDNA 89
           TV  NG+G+F +IN AIAAAPN    SN  F I+V+ G Y E +E+P  K+++ +IGD  
Sbjct: 247 TVIQNGTGNFTTINAAIAAAPNKTDGSNGYFLIYVTAGLYEEYVEVPKNKRYVMMIGDGI 306

Query: 90  FTTIIVGNRSNGTGFKTNNSATLTVKGDNFMAQHLTFENSAGPHNGQAVAVLDQADHTAY 149
             T+I GNRS   G+ T NSAT  + G NF+  ++T  N+AGP  GQAVA+    D + +
Sbjct: 307 NQTVITGNRSVVDGWTTFNSATFILSGPNFIGVNITIRNTAGPTKGQAVALRSGGDLSVF 366

Query: 150 YKCRFMGFQDTLYVKGHRQFFKECEVYGSVDFIFGSGLVLFQDTNIYAR--LQSVDVTVT 209
           Y C F  +QDTLY    RQF++EC+VYG+VDFIFG+  V+ Q+ N+Y R   +     VT
Sbjct: 367 YSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNLYPRQPRKGQSNEVT 426

Query: 210 AQSKERSMSSSGFSFQNCTVMVSPEIKANKSNVKIYLGRPWKQYSSVVFMESFLDDVVDP 269
           AQ +     ++G +   CT+  + ++  +   VK YLGRPWK+YS  V M++++D  ++P
Sbjct: 427 AQGRTDPNQNTGTAIHGCTIRPADDLATSNYTVKTYLGRPWKEYSRTVVMQTYIDGFLEP 486

Query: 270 KGWMIWEG-VPVNNLFYAEFNNRGPGADVANRVNWPGFHVIDKKSAKKFTIEYFINGTYW 329
            GW  W G   ++ L+YAE+NN GPG+D  NRV WPG+HVI+   A  FT+  F+ G  W
Sbjct: 487 SGWNAWSGDFALSTLYYAEYNNTGPGSDTTNRVTWPGYHVINATDASNFTVTNFLVGEGW 546

Query: 330 LPETGVPFRLGLLS 338
           + +TGVPF  GL++
Sbjct: 547 IGQTGVPFVGGLIA 560

BLAST of CaUC03G053360 vs. ExPASy TrEMBL
Match: A0A6J1GM54 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111455508 PE=4 SV=1)

HSP 1 Score: 506.5 bits (1303), Expect = 8.4e-140
Identity = 247/335 (73.73%), Postives = 281/335 (83.88%), Query Frame = 0

Query: 1   MAFFQLVACCVIICFFNSLVLIDSINFNATVSLNGSGDFKSINDAIAAAPNHSNTRFYIH 60
           MAF QL A C+   F N L L+DSI FNA VSL+GSGDFKSI++AIAAAPN+SNTRFYI 
Sbjct: 1   MAFLQL-AACLFFFFANVLALVDSIRFNAVVSLDGSGDFKSISEAIAAAPNNSNTRFYIR 60

Query: 61  VSPGSYNERLEIPSAKKFIALIGDNAFTTIIVGNRSNGTGFKTNNSATLTVKGDNFMAQH 120
           V+PG Y   LEIP AKKFIALIGD+A TTIIV NRS+GTGFKT+ SATL V GDNFMAQH
Sbjct: 61  VTPGIYRGHLEIPPAKKFIALIGDSALTTIIVDNRSHGTGFKTSESATLVVNGDNFMAQH 120

Query: 121 LTFENSAGPHNGQAVAVLDQADHTAYYKCRFMGFQDTLYVKGHRQFFKECEVYGSVDFIF 180
           L+FENSA P NGQAVAVLD A H+ YY CRFM FQDTLY KG  QFF+EC++YG+VDFIF
Sbjct: 121 LSFENSAEPQNGQAVAVLDYAKHSVYYDCRFMSFQDTLYSKGEFQFFRECDIYGTVDFIF 180

Query: 181 GSGLVLFQDTNIYARLQSVDVTVTAQSKERSMSSSGFSFQNCTVMVSPEIKANKSNVKIY 240
           G+GL +FQD NIYARL   ++TVTAQSKE+S+S SGFSFQNCTV VSPEI ANK+ VKIY
Sbjct: 181 GNGLAVFQDCNIYARLPMDEITVTAQSKEQSVSQSGFSFQNCTVTVSPEITANKTKVKIY 240

Query: 241 LGRPWKQYSSVVFMESFLDDVVDPKGWMIWEGVPVNNLFYAEFNNRGPGADVANRVNWPG 300
           LGRPW++YS+VVFMESFLDD V P+GW+ WEGVP+NNLFYAEFNNRGPGA+  +RVNWP 
Sbjct: 241 LGRPWREYSTVVFMESFLDDNVQPQGWISWEGVPINNLFYAEFNNRGPGANTTHRVNWPT 300

Query: 301 FHVIDKKSAKKFTIEYFINGTYWLPETGVPFRLGL 336
           FHV+DK  AK FT+E FING+ WLPET VPFR GL
Sbjct: 301 FHVLDKLLAKNFTVENFINGSDWLPETHVPFRTGL 334

BLAST of CaUC03G053360 vs. ExPASy TrEMBL
Match: A0A5A7ULX7 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold80G001290 PE=4 SV=1)

HSP 1 Score: 500.0 bits (1286), Expect = 7.9e-138
Identity = 236/319 (73.98%), Postives = 277/319 (86.83%), Query Frame = 0

Query: 19  LVLIDSINFNATVSLNGSGDFKSINDAIAAAPNHSNTRFYIHVSPGSYNERLEIPSAKKF 78
           ++ + SI+FN TVSL+GSG+FK I+DAI AAPN+SNTRFYIHV+PG+Y+E ++IP AKKF
Sbjct: 1   MLFVHSISFNLTVSLDGSGNFKRISDAIVAAPNNSNTRFYIHVAPGTYHEAIQIPKAKKF 60

Query: 79  IALIGDNAFTTIIVGNRSNGTGFKTNNSATLTVKGDNFMAQHLTFENSAGPHNGQAVAVL 138
           IALIGD+AFTTII+ NRSNGTGFKTNNSATLTV+GDNFMA+ LTFENSAGP NGQAVAV 
Sbjct: 61  IALIGDDAFTTIIIDNRSNGTGFKTNNSATLTVQGDNFMAKSLTFENSAGPQNGQAVAVF 120

Query: 139 DQADHTAYYKCRFMGFQDTLYVKGHRQFFKECEVYGSVDFIFGSGLVLFQDTNIYARLQS 198
           D+ADHTAYYKCRF+GFQDTLYV G  QFFKE  +YGSVDFIFG G V+FQD NIYAR+ S
Sbjct: 121 DKADHTAYYKCRFLGFQDTLYVNGKYQFFKESNIYGSVDFIFGHGRVMFQDCNIYARMPS 180

Query: 199 VDVTVTAQSKERSMSSSGFSFQNCTVMVSPEIKANKSNVKIYLGRPWKQYSSVVFMESFL 258
             +TVTAQSK+   S SGFSFQNCTV VSPEI +NK NVK++LGRPW+QYS+VVFMESFL
Sbjct: 181 NSITVTAQSKDFLRSISGFSFQNCTVTVSPEISSNKQNVKVFLGRPWRQYSNVVFMESFL 240

Query: 259 DDVVDPKGWMIWEGVPVNNLFYAEFNNRGPGADVANRVNWPGFHVIDKKSAKKFTIEYFI 318
           DDVV  +GWM W+GVPVNNLFY EFNN GPGADV+ RVNW G+H++DKKSA  FT++ FI
Sbjct: 241 DDVVASEGWMEWKGVPVNNLFYGEFNNFGPGADVSKRVNWTGYHLLDKKSAIGFTVDNFI 300

Query: 319 NGTYWLPETGVPFRLGLLS 338
           +G+ WL ETG+PFR GLL+
Sbjct: 301 DGSEWLAETGIPFRRGLLT 319

BLAST of CaUC03G053360 vs. ExPASy TrEMBL
Match: A0A6J1GK92 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111455081 PE=4 SV=1)

HSP 1 Score: 495.0 bits (1273), Expect = 2.5e-136
Identity = 240/337 (71.22%), Postives = 281/337 (83.38%), Query Frame = 0

Query: 1   MAFFQLVACCVIICFFNSLVLIDSINFNATVSLNGSGDFKSINDAIAAAPNHSNTRFYIH 60
           MAFFQ ++ C  +CF N L ++DS +FNATV+L+G+G+FKSI DAIAAAPN+SNTRFYI 
Sbjct: 1   MAFFQRIS-CFFLCFLNFLAVVDSTHFNATVALDGTGNFKSITDAIAAAPNNSNTRFYIR 60

Query: 61  VSPGSYNERLEIPSAKKFIALIGDNAFTTIIVGNRSNGTGFKTNNSATLTVKGDNFMAQH 120
           VSPG Y+E LEI SAKKFIALIG+N FTTIIV NRSN  GF+T+ SAT+ VKG+NFMAQH
Sbjct: 61  VSPGIYHEHLEIASAKKFIALIGENEFTTIIVDNRSNAGGFRTSESATMVVKGNNFMAQH 120

Query: 121 LTFENSAGPHNGQAVAVLDQADHTAYYKCRFMGFQDTLYVKGHRQFFKECEVYGSVDFIF 180
           L+FENSA   +GQAVAVLD A +T YYKCRF+ FQDTLY KG  QFFK C++YG+VDFIF
Sbjct: 121 LSFENSADHQDGQAVAVLDDAKYTVYYKCRFLSFQDTLYSKGEYQFFKGCDIYGTVDFIF 180

Query: 181 GSGLVLFQDTNIYARLQSVDVTVTAQSKERSMSSSGFSFQNCTVMVSPEIKANKSNVKIY 240
           G+GL +FQD N+YARL S D TVTAQSK +  + SGFSFQNCTV VSPEI + K+ VKIY
Sbjct: 181 GNGLAMFQDCNVYARLPSNDSTVTAQSK-KQWTQSGFSFQNCTVTVSPEIASCKALVKIY 240

Query: 241 LGRPWKQYSSVVFMESFLDDVVDPKGWMIWEGVPVNNLFYAEFNNRGPGADVANRVNWPG 300
           LGRPWK YS+VVFMESFLDD V P+GWM+W GVPVNNLFYAE+NNRGPGA+  +RVNWPG
Sbjct: 241 LGRPWKPYSTVVFMESFLDDNVQPQGWMLWPGVPVNNLFYAEYNNRGPGANTTHRVNWPG 300

Query: 301 FHVIDKKSAKKFTIEYFINGTYWLPETGVPFRLGLLS 338
           FHVID++ AK FT+E FINGTYWLPET VP+  GL S
Sbjct: 301 FHVIDRQLAKSFTVENFINGTYWLPETNVPYTSGLYS 335

BLAST of CaUC03G053360 vs. ExPASy TrEMBL
Match: A0A0A0LR14 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_1G049450 PE=4 SV=1)

HSP 1 Score: 491.5 bits (1264), Expect = 2.8e-135
Identity = 238/337 (70.62%), Postives = 280/337 (83.09%), Query Frame = 0

Query: 1   MAFFQLVACCVIICFFNSLVLIDSINFNATVSLNGSGDFKSINDAIAAAPNHSNTRFYIH 60
           M FF  V C  + CFFN ++LI S++FN TVSL+GSG+FK I+DAIAAAPN+SNTRFYIH
Sbjct: 1   MLFFFGVPCS-LFCFFNLMLLIHSLSFNLTVSLDGSGNFKRISDAIAAAPNNSNTRFYIH 60

Query: 61  VSPGSYNERLEIPSAKKFIALIGDNAFTTIIVGNRSNGTGFKTNNSATLTVKGDNFMAQH 120
           V+PG+Y+E L+IP  KKFIAL+GD+ FTTII+ NRSNGTGFKTNNSATLTV+GDNFMA+ 
Sbjct: 61  VAPGTYHETLQIPRLKKFIALVGDDPFTTIIIDNRSNGTGFKTNNSATLTVQGDNFMAES 120

Query: 121 LTFENSAGPHNGQAVAVLDQADHTAYYKCRFMGFQDTLYVKGHRQFFKECEVYGSVDFIF 180
           LTFENSAGP N QAVAV D+A+HTAYYKCRF+ FQDTLYV G  QFFKE ++YGSVDFI 
Sbjct: 121 LTFENSAGPQNNQAVAVFDKANHTAYYKCRFLSFQDTLYVNGKPQFFKESDIYGSVDFIC 180

Query: 181 GSGLVLFQDTNIYARLQSVDVTVTAQSKERSMSSSGFSFQNCTVMVSPEIKANKSNVKIY 240
           G G V+FQD NIYAR+    +TVTAQSK    S SGFSFQNCTV VS EI +NK NVK++
Sbjct: 181 GYGQVMFQDCNIYARMPINSITVTAQSKYILRSVSGFSFQNCTVTVSREISSNKQNVKVF 240

Query: 241 LGRPWKQYSSVVFMESFLDDVVDPKGWMIWEGVPVNNLFYAEFNNRGPGADVANRVNWPG 300
           LGRPWKQYS VVFMESFLDDVV  +GW+ W GVPVNNLFY EFNN GPGADV+ RVNW  
Sbjct: 241 LGRPWKQYSKVVFMESFLDDVVASEGWVEWIGVPVNNLFYGEFNNCGPGADVSKRVNWTS 300

Query: 301 FHVIDKKSAKKFTIEYFINGTYWLPETGVPFRLGLLS 338
           +H++DK+SA +FT++ F+NG+ WLPETG+PFR GL S
Sbjct: 301 YHLLDKESALRFTVDNFVNGSEWLPETGIPFRRGLHS 336

BLAST of CaUC03G053360 vs. ExPASy TrEMBL
Match: A0A6J1GMM0 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111455349 PE=4 SV=1)

HSP 1 Score: 485.3 bits (1248), Expect = 2.0e-133
Identity = 235/335 (70.15%), Postives = 276/335 (82.39%), Query Frame = 0

Query: 1   MAFFQLVACCVIICFFNSLVLIDSINFNATVSLNGSGDFKSINDAIAAAPNHSNTRFYIH 60
           MAF    A C+   F N L  +D I FNA VSL+GSGDFKSI++AIAAAP++SNTRFYI 
Sbjct: 1   MAFLSF-AACLFFFFANILAHVDHIRFNAVVSLDGSGDFKSISEAIAAAPDNSNTRFYIR 60

Query: 61  VSPGSYNERLEIPSAKKFIALIGDNAFTTIIVGNRSNGTGFKTNNSATLTVKGDNFMAQH 120
           VSPG+Y   LEIP  KKFIALIGDNA TTIIV NRS+GTGF T +SATL V GD++MAQH
Sbjct: 61  VSPGTYRGHLEIPPNKKFIALIGDNALTTIIVDNRSHGTGFSTTDSATLVVNGDHYMAQH 120

Query: 121 LTFENSAGPHNGQAVAVLDQADHTAYYKCRFMGFQDTLYVKGHRQFFKECEVYGSVDFIF 180
           L+FENSA   NGQAVAVL+ +  T YYKCRF+ FQDTLY+KG  QFF+EC++YG+VDFIF
Sbjct: 121 LSFENSAEHQNGQAVAVLNHSKFTTYYKCRFLSFQDTLYLKGAYQFFRECDIYGTVDFIF 180

Query: 181 GSGLVLFQDTNIYARLQSVDVTVTAQSKERSMSSSGFSFQNCTVMVSPEIKANKSNVKIY 240
           G+GL +FQD NIYARLQS ++TVTAQSKE+S   SGFSFQNCTV VSP+I + K +VKI+
Sbjct: 181 GNGLAMFQDCNIYARLQSNEITVTAQSKEQSWVKSGFSFQNCTVTVSPDIASRKVDVKIF 240

Query: 241 LGRPWKQYSSVVFMESFLDDVVDPKGWMIWEGVPVNNLFYAEFNNRGPGADVANRVNWPG 300
           LGRPW+QYS+VVFMESFLDD V P+GW++WEGVP+NNLFYAEFNNRGPGAD  +RVNW  
Sbjct: 241 LGRPWRQYSTVVFMESFLDDNVQPEGWVLWEGVPINNLFYAEFNNRGPGADTTHRVNWTS 300

Query: 301 FHVIDKKSAKKFTIEYFINGTYWLPETGVPFRLGL 336
           FHV+DK+ AK FT+E FING+ WLPET VPFR GL
Sbjct: 301 FHVLDKQLAKNFTVENFINGSDWLPETHVPFRTGL 334

BLAST of CaUC03G053360 vs. TAIR 10
Match: AT4G02300.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 301.6 bits (771), Expect = 7.9e-82
Identity = 142/319 (44.51%), Postives = 214/319 (67.08%), Query Frame = 0

Query: 22  IDSINFNATVSLNGSGDFKSINDAIAAAPNHSNTRFYIHVSPGSYNERLEIPSAKKFIAL 81
           +   N+N +V+++G+G+F +INDA+ AAPN S TRF I++  G Y E +E+P  K  I  
Sbjct: 215 VQETNYNLSVAIDGTGNFTTINDAVFAAPNMSETRFIIYIKGGEYFENVELPKKKTMIMF 274

Query: 82  IGDNAFTTIIVGNRSNGTGFKTNNSATLTVKGDNFMAQHLTFENSAGPHNGQAVAVLDQA 141
           IGD    T+I  NRS   G+ T  + T+ VKG  ++A+ ++F NSAGP   QAVA    +
Sbjct: 275 IGDGIGKTVIKANRSRIDGWSTFQTPTVGVKGKGYIAKDISFVNSAGPAKAQAVAFRSGS 334

Query: 142 DHTAYYKCRFMGFQDTLYVKGHRQFFKECEVYGSVDFIFGSGLVLFQDTNIYARLQSV-- 201
           DH+A+Y+C F G+QDTLYV   +QF++EC++YG++DFIFG+  V+FQ++++YAR  +   
Sbjct: 335 DHSAFYRCEFDGYQDTLYVHSAKQFYRECDIYGTIDFIFGNAAVVFQNSSLYARKPNPGH 394

Query: 202 DVTVTAQSKERSMSSSGFSFQNCTVMVSPEIKANKSNVKIYLGRPWKQYSSVVFMESFLD 261
            +  TAQS+ +S   +G S  NC ++ +P++   K N K YLGRPW++YS  V ++SF+D
Sbjct: 395 KIAFTAQSRNQSDQPTGISILNCRILAAPDLIPVKENFKAYLGRPWRKYSRTVIIKSFID 454

Query: 262 DVVDPKGWMIWEG---VPVNNLFYAEFNNRGPGADVANRVNWPGFHVIDKKS-AKKFTIE 321
           D++ P GW+  EG     +  L+Y E+ N GPGA++A RV WPGF  I+ ++ A +FT+ 
Sbjct: 455 DLIHPAGWL--EGKKDFALETLYYGEYMNEGPGANMAKRVTWPGFRRIENQTEATQFTVG 514

Query: 322 YFINGTYWLPETGVPFRLG 335
            FI+G+ WL  TG+PF LG
Sbjct: 515 PFIDGSTWLNSTGIPFSLG 531

BLAST of CaUC03G053360 vs. TAIR 10
Match: AT4G02330.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 291.6 bits (745), Expect = 8.2e-79
Identity = 141/324 (43.52%), Postives = 210/324 (64.81%), Query Frame = 0

Query: 20  VLIDSINFNATVSLNGSGDFKSINDAIAAAPNHSNTR---FYIHVSPGSYNERLEIPSAK 79
           VL+  I    TV+ NG+G+F +I +A+ +APN ++     F I+V+ G Y E + I   K
Sbjct: 253 VLVSDI---VTVNQNGTGNFTTITEAVNSAPNKTDGTAGYFVIYVTSGVYEENVVIAKNK 312

Query: 80  KFIALIGDNAFTTIIVGNRSNGTGFKTNNSATLTVKGDNFMAQHLTFENSAGPHNGQAVA 139
           +++ +IGD    T++ GNR+   G+ T NSAT  V   NF+A ++TF N+AGP   QAVA
Sbjct: 313 RYLMMIGDGINRTVVTGNRNVVDGWTTFNSATFAVTSPNFVAVNMTFRNTAGPEKHQAVA 372

Query: 140 VLDQADHTAYYKCRFMGFQDTLYVKGHRQFFKECEVYGSVDFIFGSGLVLFQDTNIYAR- 199
           +   AD + +Y C F  +QDTLY    RQF++EC++YG+VDFIFG+  V+FQD N+Y R 
Sbjct: 373 MRSSADLSIFYSCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQDCNLYPRQ 432

Query: 200 -LQSVDVTVTAQSKERSMSSSGFSFQNCTVMVSPEIKANKSNVKIYLGRPWKQYSSVVFM 259
            +Q+    +TAQ +     ++G S  NCT+  + ++ ++   VK YLGRPWK+YS  VFM
Sbjct: 433 PMQNQFNAITAQGRTDPNQNTGISIHNCTIKPADDLVSSNYTVKTYLGRPWKEYSRTVFM 492

Query: 260 ESFLDDVVDPKGWMIWEG-VPVNNLFYAEFNNRGPGADVANRVNWPGFHVIDKKSAKKFT 319
           +S++D+VV+P GW  W G   ++ L+YAE+NN G G+   +RV WPG+HVI+   A  FT
Sbjct: 493 QSYIDEVVEPVGWREWNGDFALSTLYYAEYNNTGSGSSTTDRVVWPGYHVINSTDANNFT 552

Query: 320 IEYFINGTYWLPETGVPFRLGLLS 338
           +E F+ G  W+ ++GVP+  GLLS
Sbjct: 553 VENFLLGDGWMVQSGVPYISGLLS 573

BLAST of CaUC03G053360 vs. TAIR 10
Match: AT4G02320.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 291.2 bits (744), Expect = 1.1e-78
Identity = 139/318 (43.71%), Postives = 206/318 (64.78%), Query Frame = 0

Query: 22  IDSINFNATVSLNGSGDFKSINDAIAAAPNHSNTRFYIHVSPGSYNERLEIPSAKKFIAL 81
           +D    N  V+ NG+G++ +I +AI+AAPN S TRF I++  G Y E +EIP  K  I  
Sbjct: 201 VDETKVNLVVAQNGTGNYTTIGEAISAAPNSSETRFVIYIKCGEYFENIEIPREKTMIMF 260

Query: 82  IGDNAFTTIIVGNRSNGTGFKTNNSATLTVKGDNFMAQHLTFENSAGPHNGQAVAVLDQA 141
           IGD    T+I  NRS   G+   +SAT+ V+G  F+A+ L+F N AGP   QAVA+   +
Sbjct: 261 IGDGIGRTVIKANRSYADGWTAFHSATVGVRGSGFIAKDLSFVNYAGPEKHQAVALRSSS 320

Query: 142 DHTAYYKCRFMGFQDTLYVKGHRQFFKECEVYGSVDFIFGSGLVLFQDTNIYARLQSVD- 201
           D +AYY+C F  +QDT+YV  H+QF++EC++YG+VDFIFG   V+FQ+ ++YAR  + + 
Sbjct: 321 DLSAYYRCSFESYQDTIYVHSHKQFYRECDIYGTVDFIFGDASVVFQNCSLYARRPNPNQ 380

Query: 202 -VTVTAQSKERSMSSSGFSFQNCTVMVSPEIKANKSNVKIYLGRPWKQYSSVVFMESFLD 261
            +  TAQ +E S   +G S  +  ++ +P++   ++N K YLGRPW+ YS  V M+SF+D
Sbjct: 381 KIIYTAQGRENSREPTGISIISSRILAAPDLIPVQANFKAYLGRPWQLYSRTVIMKSFID 440

Query: 262 DVVDPKGWMIW-EGVPVNNLFYAEFNNRGPGADVANRVNWPGFHVIDK-KSAKKFTIEYF 321
           D+VDP GW+ W +   +  L+Y E+ N GPG+++ NRV WPGF  I+  + A +F++  F
Sbjct: 441 DLVDPAGWLKWKDDFALETLYYGEYMNEGPGSNMTNRVQWPGFKRIETVEEASQFSVGPF 500

Query: 322 INGTYWLPETGVPFRLGL 336
           I+G  WL  T +PF L L
Sbjct: 501 IDGNKWLNSTRIPFTLDL 518

BLAST of CaUC03G053360 vs. TAIR 10
Match: AT2G47550.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 290.8 bits (743), Expect = 1.4e-78
Identity = 140/314 (44.59%), Postives = 200/314 (63.69%), Query Frame = 0

Query: 30  TVSLNGSGDFKSINDAIAAAPNH---SNTRFYIHVSPGSYNERLEIPSAKKFIALIGDNA 89
           TV  NG+G+F +IN AIAAAPN    SN  F I+V+ G Y E +E+P  K+++ +IGD  
Sbjct: 247 TVIQNGTGNFTTINAAIAAAPNKTDGSNGYFLIYVTAGLYEEYVEVPKNKRYVMMIGDGI 306

Query: 90  FTTIIVGNRSNGTGFKTNNSATLTVKGDNFMAQHLTFENSAGPHNGQAVAVLDQADHTAY 149
             T+I GNRS   G+ T NSAT  + G NF+  ++T  N+AGP  GQAVA+    D + +
Sbjct: 307 NQTVITGNRSVVDGWTTFNSATFILSGPNFIGVNITIRNTAGPTKGQAVALRSGGDLSVF 366

Query: 150 YKCRFMGFQDTLYVKGHRQFFKECEVYGSVDFIFGSGLVLFQDTNIYAR--LQSVDVTVT 209
           Y C F  +QDTLY    RQF++EC+VYG+VDFIFG+  V+ Q+ N+Y R   +     VT
Sbjct: 367 YSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNLYPRQPRKGQSNEVT 426

Query: 210 AQSKERSMSSSGFSFQNCTVMVSPEIKANKSNVKIYLGRPWKQYSSVVFMESFLDDVVDP 269
           AQ +     ++G +   CT+  + ++  +   VK YLGRPWK+YS  V M++++D  ++P
Sbjct: 427 AQGRTDPNQNTGTAIHGCTIRPADDLATSNYTVKTYLGRPWKEYSRTVVMQTYIDGFLEP 486

Query: 270 KGWMIWEG-VPVNNLFYAEFNNRGPGADVANRVNWPGFHVIDKKSAKKFTIEYFINGTYW 329
            GW  W G   ++ L+YAE+NN GPG+D  NRV WPG+HVI+   A  FT+  F+ G  W
Sbjct: 487 SGWNAWSGDFALSTLYYAEYNNTGPGSDTTNRVTWPGYHVINATDASNFTVTNFLVGEGW 546

Query: 330 LPETGVPFRLGLLS 338
           + +TGVPF  GL++
Sbjct: 547 IGQTGVPFVGGLIA 560

BLAST of CaUC03G053360 vs. TAIR 10
Match: AT1G02810.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 288.9 bits (738), Expect = 5.3e-78
Identity = 143/326 (43.87%), Postives = 209/326 (64.11%), Query Frame = 0

Query: 18  SLVLIDSINFNATVSLNGSGDFKSINDAIAAAPNHSNTR---FYIHVSPGSYNERLEIPS 77
           S+VLI  I    TVS +G+G+F +I  A+AAAPN+++     F I+V+ G Y E + I  
Sbjct: 257 SMVLISDI---VTVSQDGTGNFTNITAAVAAAPNNTDGSAGFFLIYVTAGIYEEYISIAK 316

Query: 78  AKKFIALIGDNAFTTIIVGNRSNGTGFKTNNSATLTVKGDNFMAQHLTFENSAGPHNGQA 137
            K+++ +IGD    T++ GNRS   G+ T NSAT  V   NF+A ++TF N+AGP   QA
Sbjct: 317 NKRYMMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVTAPNFVAVNITFRNTAGPEKHQA 376

Query: 138 VAVLDQADHTAYYKCRFMGFQDTLYVKGHRQFFKECEVYGSVDFIFGSGLVLFQDTNIYA 197
           VA+   AD + +Y C F  +QDTLY    RQF++EC+VYG+VDFIFG+  V+FQ+ N+Y 
Sbjct: 377 VALRSGADFSIFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVFQNCNLYP 436

Query: 198 R--LQSVDVTVTAQSKERSMSSSGFSFQNCTVMVSPEIKANKSNVKIYLGRPWKQYSSVV 257
           R  + +    +TAQ +     ++G S QNCT+  + ++ ++   VK YLGRPWK+YS  V
Sbjct: 437 RKPMPNQFNAITAQGRSDPNQNTGTSIQNCTIKPADDLVSSNYTVKTYLGRPWKEYSRTV 496

Query: 258 FMESFLDDVVDPKGWMIWEG-VPVNNLFYAEFNNRGPGADVANRVNWPGFHVIDKKSAKK 317
           +M+S++D  V+P GW  W G   ++ L+YAE+NN GPG++  NRV WPG+HVI+   A  
Sbjct: 497 YMQSYIDGFVEPVGWREWNGDFALSTLYYAEYNNTGPGSNTTNRVTWPGYHVINSTDAAN 556

Query: 318 FTIEYFINGTYWLPETGVPFRLGLLS 338
           FT+        W+ +TGVP+  GL+S
Sbjct: 557 FTVTGLFIEADWIWKTGVPYTSGLIS 579

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023511664.19.2e-14174.33probable pectinesterase/pectinesterase inhibitor 7 [Cucurbita pepo subsp. pepo][more]
KAG6572387.11.2e-14074.33putative pectinesterase/pectinesterase inhibitor 7, partial [Cucurbita argyrospe... [more]
XP_022952993.11.7e-13973.73probable pectinesterase/pectinesterase inhibitor 7 [Cucurbita moschata][more]
KAA0055286.11.6e-13773.98putative pectinesterase/pectinesterase inhibitor 47 [Cucumis melo var. makuwa][more]
XP_022952377.15.2e-13671.22probable pectinesterase/pectinesterase inhibitor 7 isoform X1 [Cucurbita moschat... [more]
Match NameE-valueIdentityDescription
O814151.1e-8044.51Probable pectinesterase/pectinesterase inhibitor 39 OS=Arabidopsis thaliana OX=3... [more]
O048871.2e-7745.98Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1[more]
Q8RXK71.2e-7743.52Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana OX=3... [more]
O813011.5e-7743.71Probable pectinesterase/pectinesterase inhibitor 40 OS=Arabidopsis thaliana OX=3... [more]
O222562.0e-7744.59Probable pectinesterase/pectinesterase inhibitor 20 OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
A0A6J1GM548.4e-14073.73Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111455508 PE=4 SV=1[more]
A0A5A7ULX77.9e-13873.98Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold80G001290... [more]
A0A6J1GK922.5e-13671.22Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111455081 PE=4 SV=1[more]
A0A0A0LR142.8e-13570.62Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_1G049450 PE=4 SV=1[more]
A0A6J1GMM02.0e-13370.15Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111455349 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT4G02300.17.9e-8244.51Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT4G02330.18.2e-7943.52Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT4G02320.11.1e-7843.71Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT2G47550.11.4e-7844.59Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT1G02810.15.3e-7843.87Plant invertase/pectin methylesterase inhibitor superfamily [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (USVL246-FR2) v1
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 28..320
e-value: 1.5E-107
score: 358.7
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 22..335
e-value: 8.8E-112
score: 375.6
NoneNo IPR availablePANTHERPTHR31707:SF205PECTINESTERASE/PECTINESTERASE INHIBITOR 22-RELATEDcoord: 24..333
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 24..333
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 172..181
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 27..334

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CaUC03G053360.1CaUC03G053360.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity