CaUC03G051660 (gene) Watermelon (USVL246-FR2) v1

Overview


Name	CaUC03G051660
Type	gene
Organism	Citrullus amarus (Watermelon (USVL246-FR2) v1)
Description	Methyltransferase
Location	Ciama_Chr03: 1921312 .. 1921797 (+)
RNA-Seq Expression	CaUC03G051660
Synteny	CaUC03G051660

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAAAAGAGTAATGGATTCTCTCCCCTTATTCCTCACCGGAACTCTCCTCGCCATCGGCCTTTTACTGGTTCTTCTTCATCTATGGGTCTGTTGAACGTGAAGGCAAACTCGCTGCCAACCTCTCTGGTGGCTCAATCTCCTCCAAGAAAATTCAAGATAAGTAGAAACGGTATTGGTCCTTTTTTCCACCGCCCTGAGAAGATCGAAACCACCAAAAAAAGGTCCCCGATTTCATCGATAGCTTTTACAACTTAGTCAGAGATATTTACAAATGGGGTTGGGGGAAAGTCCTTCCACTTTGCCCGTTCGATTCCCGGAAAATCTTACAAAGAAGCCACTCGCCTCCACGAAGAAATGGTCGACGATCTAGTCAACGTCAAACTCGATGATCGAATTCTCGATGTCGGATGTGGCGTCGGTGGTTCGATGCGGTCAATAAAAAGTAGATATCAATCATATCCTTTATGGCTGCTATCAATGTAG

mRNA sequence

ATGAAAAAGAGTAATGGATTCTCTCCCCTTATTCCTCACCGGAACTCTCCTCGCCATCGGCCTTTTACTGGTTCTTCTTCATCTATGGGTCTGTTGAACGTGAAGGCAAACTCGCTGCCAACCTCTCTGGTGGCTCAATCTCCTCCAAGAAAATTCAAGATAAGTAGAAACGAGATATTTACAAATGGGGTTGGGGGAAAGTCCTTCCACTTTGCCCGTTCGATTCCCGGAAAATCTTACAAAGAAGCCACTCGCCTCCACGAAGAAATGGTCGACGATCTAGTCAACGTCAAACTCGATGATCGAATTCTCGATGTCGGATGTGGCGTCGGTGGTTCGATGCGGTCAATAAAAAGTAGATATCAATCATATCCTTTATGGCTGCTATCAATGTAG

Coding sequence (CDS)

ATGAAAAAGAGTAATGGATTCTCTCCCCTTATTCCTCACCGGAACTCTCCTCGCCATCGGCCTTTTACTGGTTCTTCTTCATCTATGGGTCTGTTGAACGTGAAGGCAAACTCGCTGCCAACCTCTCTGGTGGCTCAATCTCCTCCAAGAAAATTCAAGATAAGTAGAAACGAGATATTTACAAATGGGGTTGGGGGAAAGTCCTTCCACTTTGCCCGTTCGATTCCCGGAAAATCTTACAAAGAAGCCACTCGCCTCCACGAAGAAATGGTCGACGATCTAGTCAACGTCAAACTCGATGATCGAATTCTCGATGTCGGATGTGGCGTCGGTGGTTCGATGCGGTCAATAAAAAGTAGATATCAATCATATCCTTTATGGCTGCTATCAATGTAG

Protein sequence

MKKSNGFSPLIPHRNSPRHRPFTGSSSSMGLLNVKANSLPTSLVAQSPPRKFKISRNEIFTNGVGGKSFHFARSIPGKSYKEATRLHEEMVDDLVNVKLDDRILDVGCGVGGSMRSIKSRYQSYPLWLLSM

Homology

[Close]

BLAST of CaUC03G051660 vs. NCBI nr
Match: XP_038899067.1 (24-methylenesterol C-methyltransferase 2 [Benincasa hispida])

HSP 1 Score: 93.6 bits (231), Expect = 1.4e-15
Identity = 45/52 (86.54%), Postives = 46/52 (88.46%), Query Frame = 0


Query: 66  GKSFHFARSIPGKSYKEATRLHEEMVDDLVNVKLDDRILDVGCGVGGSMRSI 118
           G+SFHFAR IPGKSYKEATRLHEEMV DLVN K  DRILDVGCGVGG MRSI
Sbjct: 90  GQSFHFARPIPGKSYKEATRLHEEMVADLVNAKSGDRILDVGCGVGGPMRSI 141

[Close]

BLAST of CaUC03G051660 vs. NCBI nr
Match: XP_008466453.1 (PREDICTED: 24-methylenesterol C-methyltransferase 2 [Cucumis melo] >KAA0063316.1 24-methylenesterol C-methyltransferase 2 [Cucumis melo var. makuwa] >TYK31459.1 24-methylenesterol C-methyltransferase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 90.9 bits (224), Expect = 8.9e-15
Identity = 44/52 (84.62%), Postives = 45/52 (86.54%), Query Frame = 0


Query: 66  GKSFHFARSIPGKSYKEATRLHEEMVDDLVNVKLDDRILDVGCGVGGSMRSI 118
           G+SFHFAR IPGKSYKEATRLHEEMV DLV  K  DRILDVGCGVGG MRSI
Sbjct: 90  GQSFHFARPIPGKSYKEATRLHEEMVADLVKAKPGDRILDVGCGVGGPMRSI 141

[Close]

BLAST of CaUC03G051660 vs. NCBI nr
Match: XP_004136483.1 (24-methylenesterol C-methyltransferase 2 [Cucumis sativus] >KGN60066.1 hypothetical protein Csa_002528 [Cucumis sativus])

HSP 1 Score: 89.7 bits (221), Expect = 2.0e-14
Identity = 43/52 (82.69%), Postives = 45/52 (86.54%), Query Frame = 0


Query: 66  GKSFHFARSIPGKSYKEATRLHEEMVDDLVNVKLDDRILDVGCGVGGSMRSI 118
           G+SFHFAR IPGK+YKEATRLHEEMV DLV  K  DRILDVGCGVGG MRSI
Sbjct: 90  GQSFHFARPIPGKTYKEATRLHEEMVADLVKAKPGDRILDVGCGVGGPMRSI 141

[Close]

BLAST of CaUC03G051660 vs. NCBI nr
Match: XP_026455971.1 (24-methylenesterol C-methyltransferase 2-like [Papaver somniferum] >XP_026455972.1 24-methylenesterol C-methyltransferase 2-like [Papaver somniferum])

HSP 1 Score: 87.0 bits (214), Expect = 1.3e-13
Identity = 40/54 (74.07%), Postives = 47/54 (87.04%), Query Frame = 0


Query: 66  GKSFHFARSIPGKSYKEATRLHEEMVDDLVNVKLDDRILDVGCGVGGSMRSIKS 120
           G+SFHF+ SIPGKS+K+ATRLHEEM  DL+NVK  D+ILDVGCGVGG MR+I S
Sbjct: 91  GQSFHFSPSIPGKSHKDATRLHEEMAVDLINVKSGDKILDVGCGVGGPMRAIAS 144

[Close]

BLAST of CaUC03G051660 vs. NCBI nr
Match: KAG6591249.1 (24-methylenesterol C-methyltransferase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 87.0 bits (214), Expect = 1.3e-13
Identity = 43/52 (82.69%), Postives = 44/52 (84.62%), Query Frame = 0


Query: 66  GKSFHFARSIPGKSYKEATRLHEEMVDDLVNVKLDDRILDVGCGVGGSMRSI 118
           G+SFHFAR IPGKS KEATRLHEEMV DLV  K  DRILDVGCGVGG MRSI
Sbjct: 90  GQSFHFARPIPGKSDKEATRLHEEMVADLVKAKPGDRILDVGCGVGGPMRSI 141

[Close]

BLAST of CaUC03G051660 vs. ExPASy Swiss-Prot
Match: Q39227 (24-methylenesterol C-methyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=SMT2 PE=1 SV=2)

HSP 1 Score: 82.0 bits (201), Expect = 5.4e-15
Identity = 38/54 (70.37%), Postives = 45/54 (83.33%), Query Frame = 0


Query: 66  GKSFHFARSIPGKSYKEATRLHEEMVDDLVNVKLDDRILDVGCGVGGSMRSIKS 120
           G+SFHF+ SIPGKS+K+ATRLHEEM  DL+ VK   +ILDVGCGVGG MR+I S
Sbjct: 90  GQSFHFSPSIPGKSHKDATRLHEEMAVDLIQVKPGQKILDVGCGVGGPMRAIAS 143

[Close]

BLAST of CaUC03G051660 vs. ExPASy Swiss-Prot
Match: O82427 (24-methylenesterol C-methyltransferase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Smt2-1 PE=2 SV=2)

HSP 1 Score: 74.7 bits (182), Expect = 8.6e-13
Identity = 33/52 (63.46%), Postives = 43/52 (82.69%), Query Frame = 0


Query: 66  GKSFHFARSIPGKSYKEATRLHEEMVDDLVNVKLDDRILDVGCGVGGSMRSI 118
           G+SFHF+ S+PG+S++EATR+HEE V DL+  K   R+LDVGCGVGG MR+I
Sbjct: 95  GQSFHFSPSLPGRSHREATRVHEERVADLLQAKPGHRLLDVGCGVGGPMRAI 146

[Close]

BLAST of CaUC03G051660 vs. ExPASy Swiss-Prot
Match: Q94JS4 (24-methylenesterol C-methyltransferase 3 OS=Arabidopsis thaliana OX=3702 GN=SMT3 PE=2 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 1.5e-12
Identity = 33/52 (63.46%), Postives = 41/52 (78.85%), Query Frame = 0


Query: 66  GKSFHFARSIPGKSYKEATRLHEEMVDDLVNVKLDDRILDVGCGVGGSMRSI 118
           G+SFHF+  +PGKS K+ATR+HEEM  DL+ VK   +ILD GCGVGG MR+I
Sbjct: 90  GQSFHFSPHVPGKSDKDATRIHEEMAVDLIKVKPGQKILDAGCGVGGPMRAI 141

[Close]

BLAST of CaUC03G051660 vs. ExPASy Swiss-Prot
Match: Q875K1 (Sterol 24-C-methyltransferase OS=Clavispora lusitaniae (strain ATCC 42720) OX=306902 GN=ERG6 PE=3 SV=1)

HSP 1 Score: 55.5 bits (132), Expect = 5.4e-07
Identity = 24/52 (46.15%), Postives = 35/52 (67.31%), Query Frame = 0


Query: 66  GKSFHFARSIPGKSYKEATRLHEEMVDDLVNVKLDDRILDVGCGVGGSMRSI 118
           G SFHF+R   G+++++AT  HE  +   +N+  + R+LDVGCGVGG  R I
Sbjct: 89  GSSFHFSRYYKGEAFRQATARHEHFLAHKMNINENMRVLDVGCGVGGPGREI 140

[Close]

BLAST of CaUC03G051660 vs. ExPASy Swiss-Prot
Match: Q9P3R1 (Sterol 24-C-methyltransferase erg-4 OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) OX=367110 GN=erg-4 PE=3 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 7.1e-07
Identity = 24/52 (46.15%), Postives = 33/52 (63.46%), Query Frame = 0


Query: 66  GKSFHFARSIPGKSYKEATRLHEEMVDDLVNVKLDDRILDVGCGVGGSMRSI 118
           G+ FHF R  PG+S+ +A   HE  +   + +K D ++LDVGCGVGG  R I
Sbjct: 95  GQCFHFCRYSPGESFYQAIARHEHYLAAQIGIKKDMKVLDVGCGVGGPAREI 146

[Close]

BLAST of CaUC03G051660 vs. ExPASy TrEMBL
Match: A0A5A7VA53 (Methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G007740 PE=3 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 4.3e-15
Identity = 44/52 (84.62%), Postives = 45/52 (86.54%), Query Frame = 0


Query: 66  GKSFHFARSIPGKSYKEATRLHEEMVDDLVNVKLDDRILDVGCGVGGSMRSI 118
           G+SFHFAR IPGKSYKEATRLHEEMV DLV  K  DRILDVGCGVGG MRSI
Sbjct: 90  GQSFHFARPIPGKSYKEATRLHEEMVADLVKAKPGDRILDVGCGVGGPMRSI 141

[Close]

BLAST of CaUC03G051660 vs. ExPASy TrEMBL
Match: A0A1S3CRG4 (Methyltransferase OS=Cucumis melo OX=3656 GN=LOC103503852 PE=3 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 4.3e-15
Identity = 44/52 (84.62%), Postives = 45/52 (86.54%), Query Frame = 0


Query: 66  GKSFHFARSIPGKSYKEATRLHEEMVDDLVNVKLDDRILDVGCGVGGSMRSI 118
           G+SFHFAR IPGKSYKEATRLHEEMV DLV  K  DRILDVGCGVGG MRSI
Sbjct: 90  GQSFHFARPIPGKSYKEATRLHEEMVADLVKAKPGDRILDVGCGVGGPMRSI 141

[Close]

BLAST of CaUC03G051660 vs. ExPASy TrEMBL
Match: A0A0A0LJ82 (Methyltransferase OS=Cucumis sativus OX=3659 GN=Csa_3G875420 PE=3 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 9.6e-15
Identity = 43/52 (82.69%), Postives = 45/52 (86.54%), Query Frame = 0


Query: 66  GKSFHFARSIPGKSYKEATRLHEEMVDDLVNVKLDDRILDVGCGVGGSMRSI 118
           G+SFHFAR IPGK+YKEATRLHEEMV DLV  K  DRILDVGCGVGG MRSI
Sbjct: 90  GQSFHFARPIPGKTYKEATRLHEEMVADLVKAKPGDRILDVGCGVGGPMRSI 141

[Close]

BLAST of CaUC03G051660 vs. ExPASy TrEMBL
Match: A0A6J1IGM5 (Methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111476770 PE=3 SV=1)

HSP 1 Score: 87.0 bits (214), Expect = 6.2e-14
Identity = 43/52 (82.69%), Postives = 44/52 (84.62%), Query Frame = 0


Query: 66  GKSFHFARSIPGKSYKEATRLHEEMVDDLVNVKLDDRILDVGCGVGGSMRSI 118
           G+SFHFAR IPGKS KEATRLHEEMV DLV  K  DRILDVGCGVGG MRSI
Sbjct: 90  GQSFHFARPIPGKSDKEATRLHEEMVADLVKAKPGDRILDVGCGVGGPMRSI 141

[Close]

BLAST of CaUC03G051660 vs. ExPASy TrEMBL
Match: A0A200QEJ8 (Methyltransferase OS=Macleaya cordata OX=56857 GN=BVC80_8809g21 PE=3 SV=1)

HSP 1 Score: 87.0 bits (214), Expect = 6.2e-14
Identity = 40/58 (68.97%), Postives = 49/58 (84.48%), Query Frame = 0


Query: 66  GKSFHFARSIPGKSYKEATRLHEEMVDDLVNVKLDDRILDVGCGVGGSMRSIKSRYQS 124
           G+SFHF+ SIPGKS+K+ATR+HEEM  DL+NVK  DRILDVGCGVGG MR+I +  Q+
Sbjct: 90  GQSFHFSPSIPGKSHKDATRIHEEMAVDLLNVKPGDRILDVGCGVGGPMRAIAAHSQA 147

[Close]

BLAST of CaUC03G051660 vs. TAIR 10
Match: AT1G20330.1 (sterol methyltransferase 2 )

HSP 1 Score: 82.0 bits (201), Expect = 3.8e-16
Identity = 38/54 (70.37%), Postives = 45/54 (83.33%), Query Frame = 0


Query: 66  GKSFHFARSIPGKSYKEATRLHEEMVDDLVNVKLDDRILDVGCGVGGSMRSIKS 120
           G+SFHF+ SIPGKS+K+ATRLHEEM  DL+ VK   +ILDVGCGVGG MR+I S
Sbjct: 90  GQSFHFSPSIPGKSHKDATRLHEEMAVDLIQVKPGQKILDVGCGVGGPMRAIAS 143

[Close]

BLAST of CaUC03G051660 vs. TAIR 10
Match: AT1G76090.1 (sterol methyltransferase 3 )

HSP 1 Score: 73.9 bits (180), Expect = 1.0e-13
Identity = 33/52 (63.46%), Postives = 41/52 (78.85%), Query Frame = 0


Query: 66  GKSFHFARSIPGKSYKEATRLHEEMVDDLVNVKLDDRILDVGCGVGGSMRSI 118
           G+SFHF+  +PGKS K+ATR+HEEM  DL+ VK   +ILD GCGVGG MR+I
Sbjct: 90  GQSFHFSPHVPGKSDKDATRIHEEMAVDLIKVKPGQKILDAGCGVGGPMRAI 141

[Close]

BLAST of CaUC03G051660 vs. TAIR 10
Match: AT5G13710.1 (sterol methyltransferase 1 )

HSP 1 Score: 51.2 bits (121), Expect = 7.2e-07
Identity = 21/52 (40.38%), Postives = 35/52 (67.31%), Query Frame = 0


Query: 66  GKSFHFARSIPGKSYKEATRLHEEMVDDLVNVKLDDRILDVGCGVGGSMRSI 118
           G+SFHFA+   G+S +E+ + HE  +   + ++   ++LDVGCG+GG +R I
Sbjct: 61  GESFHFAQRWKGESLRESIKRHEHFLALQLGIQPGQKVLDVGCGIGGPLREI 112

[Close]

BLAST of CaUC03G051660 vs. TAIR 10
Match: AT5G13710.2 (sterol methyltransferase 1 )

HSP 1 Score: 51.2 bits (121), Expect = 7.2e-07
Identity = 21/52 (40.38%), Postives = 35/52 (67.31%), Query Frame = 0


Query: 66  GKSFHFARSIPGKSYKEATRLHEEMVDDLVNVKLDDRILDVGCGVGGSMRSI 118
           G+SFHFA+   G+S +E+ + HE  +   + ++   ++LDVGCG+GG +R I
Sbjct: 61  GESFHFAQRWKGESLRESIKRHEHFLALQLGIQPGQKVLDVGCGIGGPLREI 112

The following BLAST results are available for this feature:

BLAST of CaUC03G051660 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038899067.1	1.4e-15	86.54	24-methylenesterol C-methyltransferase 2 [Benincasa hispida]	[more]
XP_008466453.1	8.9e-15	84.62	PREDICTED: 24-methylenesterol C-methyltransferase 2 [Cucumis melo] >KAA0063316.1...	[more]
XP_004136483.1	2.0e-14	82.69	24-methylenesterol C-methyltransferase 2 [Cucumis sativus] >KGN60066.1 hypotheti...	[more]
XP_026455971.1	1.3e-13	74.07	24-methylenesterol C-methyltransferase 2-like [Papaver somniferum] >XP_026455972...	[more]
KAG6591249.1	1.3e-13	82.69	24-methylenesterol C-methyltransferase 2, partial [Cucurbita argyrosperma subsp....	[more]

BLAST of CaUC03G051660 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q39227	5.4e-15	70.37	24-methylenesterol C-methyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=SMT2...	[more]
O82427	8.6e-13	63.46	24-methylenesterol C-methyltransferase 2 OS=Oryza sativa subsp. japonica OX=3994...	[more]
Q94JS4	1.5e-12	63.46	24-methylenesterol C-methyltransferase 3 OS=Arabidopsis thaliana OX=3702 GN=SMT3...	[more]
Q875K1	5.4e-07	46.15	Sterol 24-C-methyltransferase OS=Clavispora lusitaniae (strain ATCC 42720) OX=30...	[more]
Q9P3R1	7.1e-07	46.15	Sterol 24-C-methyltransferase erg-4 OS=Neurospora crassa (strain ATCC 24698 / 74...	[more]

BLAST of CaUC03G051660 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7VA53	4.3e-15	84.62	Methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G00...	[more]
A0A1S3CRG4	4.3e-15	84.62	Methyltransferase OS=Cucumis melo OX=3656 GN=LOC103503852 PE=3 SV=1	[more]
A0A0A0LJ82	9.6e-15	82.69	Methyltransferase OS=Cucumis sativus OX=3659 GN=Csa_3G875420 PE=3 SV=1	[more]
A0A6J1IGM5	6.2e-14	82.69	Methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111476770 PE=3 SV=1	[more]
A0A200QEJ8	6.2e-14	68.97	Methyltransferase OS=Macleaya cordata OX=56857 GN=BVC80_8809g21 PE=3 SV=1	[more]

BLAST of CaUC03G051660 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G20330.1	3.8e-16	70.37	sterol methyltransferase 2	[more]
AT1G76090.1	1.0e-13	63.46	sterol methyltransferase 3	[more]
AT5G13710.1	7.2e-07	40.38	sterol methyltransferase 1	[more]
AT5G13710.2	7.2e-07	40.38	sterol methyltransferase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (USVL246-FR2) v1
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 56..124 e-value: 7.3E-6 score: 27.3
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..27
IPR044625	Sterol methyltransferase SMT2/3-like	PANTHER	PTHR44742	FAMILY NOT NAMED	coord: 55..122
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 74..121

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CaUC03G051660.1	CaUC03G051660.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032259	methylation
biological_process	GO:0016126	sterol biosynthetic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0008168	methyltransferase activity