CaUC02G041450 (gene) Watermelon (USVL246-FR2) v1

Overview


Name	CaUC02G041450
Type	gene
Organism	Citrullus amarus (Watermelon (USVL246-FR2) v1)
Description	kunitz trypsin inhibitor 1-like
Location	Ciama_Chr02: 28749288 .. 28749875 (+)
RNA-Seq Expression	CaUC02G041450
Synteny	CaUC02G041450

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATGATGTTGCATAAGTCACTAGCCATCCTTAGCTATGTATGCCTCTTCATGGCCATAACTTCCACAGCTCAACTTCCTCCGGTGCTCGACACCGACGGTGAACCTCTTCGACGTGGTGTCGAGTACTACATCAAGCCTGCAATTACCGACGTTGCAGGCAACCTCACCCTAAAAAGTCGTAGTAATGCTCCATGCCCGCTCTATGTCGGCCAGGAAGCAGTTACTTCGACAAATATTGGCCTCCCGGTCACCTTCACACCCATCGTGGCAAGCGAAGATATAATTGAAGAAAGTAGGGGTTTGAACATCGTGTTTCAAGCGTCGTCAACGTGCATAATGTCAACACAATGGAGAGTAGATGAGGCGGAGTCTGACACCGGGAGGAGGTTTGTGGGGACCGGGAACGATGACGGTCCTACTGGGGTCTTTAGGATCGATAGGAACAATGGAGTTTACAATATAGTATGGTGTCCTGCGATGATGGGGAGGCCAAGGTGTGGAAGTGCTGGGATTTTGGTTGAGGATGGAGTAAGGCTGTTGGCTTTGGATGGTGATGCATTTCCTTTTGAGTTTGTGAAATCTTGA

mRNA sequence

ATGATGATGTTGCATAAGTCACTAGCCATCCTTAGCTATGTATGCCTCTTCATGGCCATAACTTCCACAGCTCAACTTCCTCCGGTGCTCGACACCGACGGTGAACCTCTTCGACGTGGTGTCGAGTACTACATCAAGCCTGCAATTACCGACGTTGCAGGCAACCTCACCCTAAAAAGTCGTAGTAATGCTCCATGCCCGCTCTATGTCGGCCAGGAAGCAGTTACTTCGACAAATATTGGCCTCCCGGTCACCTTCACACCCATCGTGGCAAGCGAAGATATAATTGAAGAAAGTAGGGGTTTGAACATCGTGTTTCAAGCGTCGTCAACGTGCATAATGTCAACACAATGGAGAGTAGATGAGGCGGAGTCTGACACCGGGAGGAGGTTTGTGGGGACCGGGAACGATGACGGTCCTACTGGGGTCTTTAGGATCGATAGGAACAATGGAGTTTACAATATAGTATGGTGTCCTGCGATGATGGGGAGGCCAAGGTGTGGAAGTGCTGGGATTTTGGTTGAGGATGGAGTAAGGCTGTTGGCTTTGGATGGTGATGCATTTCCTTTTGAGTTTGTGAAATCTTGA

Coding sequence (CDS)

ATGATGATGTTGCATAAGTCACTAGCCATCCTTAGCTATGTATGCCTCTTCATGGCCATAACTTCCACAGCTCAACTTCCTCCGGTGCTCGACACCGACGGTGAACCTCTTCGACGTGGTGTCGAGTACTACATCAAGCCTGCAATTACCGACGTTGCAGGCAACCTCACCCTAAAAAGTCGTAGTAATGCTCCATGCCCGCTCTATGTCGGCCAGGAAGCAGTTACTTCGACAAATATTGGCCTCCCGGTCACCTTCACACCCATCGTGGCAAGCGAAGATATAATTGAAGAAAGTAGGGGTTTGAACATCGTGTTTCAAGCGTCGTCAACGTGCATAATGTCAACACAATGGAGAGTAGATGAGGCGGAGTCTGACACCGGGAGGAGGTTTGTGGGGACCGGGAACGATGACGGTCCTACTGGGGTCTTTAGGATCGATAGGAACAATGGAGTTTACAATATAGTATGGTGTCCTGCGATGATGGGGAGGCCAAGGTGTGGAAGTGCTGGGATTTTGGTTGAGGATGGAGTAAGGCTGTTGGCTTTGGATGGTGATGCATTTCCTTTTGAGTTTGTGAAATCTTGA

Protein sequence

MMMLHKSLAILSYVCLFMAITSTAQLPPVLDTDGEPLRRGVEYYIKPAITDVAGNLTLKSRSNAPCPLYVGQEAVTSTNIGLPVTFTPIVASEDIIEESRGLNIVFQASSTCIMSTQWRVDEAESDTGRRFVGTGNDDGPTGVFRIDRNNGVYNIVWCPAMMGRPRCGSAGILVEDGVRLLALDGDAFPFEFVKS

Homology

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BLAST of CaUC02G041450 vs. NCBI nr
Match: XP_038888526.1 (kunitz type trypsin inhibitor 106-like [Benincasa hispida])

HSP 1 Score: 364.8 bits (935), Expect = 4.7e-97
Identity = 176/195 (90.26%), Postives = 184/195 (94.36%), Query Frame = 0


Query: 1   MMMLHKSLAILSYVCLFMAITSTAQLPPVLDTDGEPLRRGVEYYIKPAITDVAGNLTLKS 60
           MMMLHKSLAI  Y CLFMAITSTAQLPPVLDTD +PLRRGVEYYIKPAITDV GNLTLKS
Sbjct: 1   MMMLHKSLAIFGYACLFMAITSTAQLPPVLDTDSQPLRRGVEYYIKPAITDVGGNLTLKS 60

Query: 61  RSNAPCPLYVGQEAVTSTNIGLPVTFTPIVASEDIIEESRGLNIVFQASSTCIMSTQWRV 120
           RSNAPCPL+VGQE VTSTNIGLPVTFTPIVA EDIIEESR LNIVF+A STC+ STQWRV
Sbjct: 61  RSNAPCPLFVGQEPVTSTNIGLPVTFTPIVAGEDIIEESRSLNIVFEALSTCVTSTQWRV 120

Query: 121 DEAESDTGRRFVGTGNDDGPTGVFRIDRNNGVYNIVWCPAMMGRPRCGSAGILVEDGVRL 180
           DEAESDTGRRFVG GNDDGP+G+FRIDRNNGVYNIVWCPAMMGRPRCGSAGILVE+GVRL
Sbjct: 121 DEAESDTGRRFVGIGNDDGPSGIFRIDRNNGVYNIVWCPAMMGRPRCGSAGILVENGVRL 180

Query: 181 LALDGDAFPFEFVKS 196
           LALDGDAFPFEFVK+
Sbjct: 181 LALDGDAFPFEFVKA 195

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BLAST of CaUC02G041450 vs. NCBI nr
Match: XP_022946294.1 (miraculin-like [Cucurbita moschata] >XP_022946295.1 miraculin-like [Cucurbita moschata])

HSP 1 Score: 351.7 bits (901), Expect = 4.1e-93
Identity = 169/193 (87.56%), Postives = 180/193 (93.26%), Query Frame = 0


Query: 3   MLHKSLAILSYVCLFMAITSTAQLPPVLDTDGEPLRRGVEYYIKPAITDVAGNLTLKSRS 62
           MLH SLAI S++ +FMAITSTAQLPPVLDTDG+PLRRGVEYYIKPAITDVAGNLTLKSRS
Sbjct: 1   MLHNSLAIFSFLLVFMAITSTAQLPPVLDTDGQPLRRGVEYYIKPAITDVAGNLTLKSRS 60

Query: 63  NAPCPLYVGQEAVTSTNIGLPVTFTPIVASEDIIEESRGLNIVFQASSTCIMSTQWRVDE 122
           NAPCPLYVGQE + STNIGLPVTFTPIVA EDIIEES G N+VFQASSTCI STQWRVDE
Sbjct: 61  NAPCPLYVGQEPIASTNIGLPVTFTPIVAGEDIIEESMGFNVVFQASSTCITSTQWRVDE 120

Query: 123 AESDTGRRFVGTGNDDGPTGVFRIDRNNGVYNIVWCPAMMGRPRCGSAGILVEDGVRLLA 182
            ES TGRRFVG GND+GPTG+FRIDRNNGVYNIVWCP M+GRPRCGSAGILVEDGVRLLA
Sbjct: 121 MESATGRRFVGVGNDNGPTGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVEDGVRLLA 180

Query: 183 LDGDAFPFEFVKS 196
           LDG+AFPFEFVK+
Sbjct: 181 LDGNAFPFEFVKA 193

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BLAST of CaUC02G041450 vs. NCBI nr
Match: XP_023545477.1 (miraculin-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 348.2 bits (892), Expect = 4.6e-92
Identity = 168/193 (87.05%), Postives = 178/193 (92.23%), Query Frame = 0


Query: 3   MLHKSLAILSYVCLFMAITSTAQLPPVLDTDGEPLRRGVEYYIKPAITDVAGNLTLKSRS 62
           MLH SLAI SY+ +FMAITSTAQLPPVLDTDG+PL+RGVEYYIKPAITDVAGNLTLKSRS
Sbjct: 1   MLHNSLAIFSYLLVFMAITSTAQLPPVLDTDGQPLQRGVEYYIKPAITDVAGNLTLKSRS 60

Query: 63  NAPCPLYVGQEAVTSTNIGLPVTFTPIVASEDIIEESRGLNIVFQASSTCIMSTQWRVDE 122
           NAPCPLYVGQE V STNIGLPVTFTPIVA EDIIEES G N+ FQASSTCI STQWRVDE
Sbjct: 61  NAPCPLYVGQEPVASTNIGLPVTFTPIVAGEDIIEESMGFNVAFQASSTCITSTQWRVDE 120

Query: 123 AESDTGRRFVGTGNDDGPTGVFRIDRNNGVYNIVWCPAMMGRPRCGSAGILVEDGVRLLA 182
            ES TGRRFVG GND+ PTG+FRIDRNNGVYNIVWCP M+GRPRCGSAGILVEDGVRLLA
Sbjct: 121 MESATGRRFVGVGNDNSPTGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVEDGVRLLA 180

Query: 183 LDGDAFPFEFVKS 196
           LDG+AFPFEFVK+
Sbjct: 181 LDGNAFPFEFVKA 193

[Close]

BLAST of CaUC02G041450 vs. NCBI nr
Match: XP_022999136.1 (miraculin-like [Cucurbita maxima])

HSP 1 Score: 347.8 bits (891), Expect = 6.0e-92
Identity = 167/193 (86.53%), Postives = 179/193 (92.75%), Query Frame = 0


Query: 3   MLHKSLAILSYVCLFMAITSTAQLPPVLDTDGEPLRRGVEYYIKPAITDVAGNLTLKSRS 62
           MLH SLAI SY+ +F+AITSTAQLPPVLDTDG+PLRRGVEYYIKPAIT+VAGNLTLK+RS
Sbjct: 1   MLHNSLAIFSYLLVFLAITSTAQLPPVLDTDGQPLRRGVEYYIKPAITNVAGNLTLKNRS 60

Query: 63  NAPCPLYVGQEAVTSTNIGLPVTFTPIVASEDIIEESRGLNIVFQASSTCIMSTQWRVDE 122
           NAPCPLYVGQE V STNIGLPVTFTPIVA EDIIEES G N+ FQASSTCI STQWRVDE
Sbjct: 61  NAPCPLYVGQEPVASTNIGLPVTFTPIVAGEDIIEESMGFNVAFQASSTCITSTQWRVDE 120

Query: 123 AESDTGRRFVGTGNDDGPTGVFRIDRNNGVYNIVWCPAMMGRPRCGSAGILVEDGVRLLA 182
            ES TGRRFVG GND+GPTG+FRIDRNNGVYNIVWCP M+GRPRCGSAGILVEDGVRLLA
Sbjct: 121 MESATGRRFVGVGNDNGPTGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVEDGVRLLA 180

Query: 183 LDGDAFPFEFVKS 196
           LDG+AFPFEFVK+
Sbjct: 181 LDGNAFPFEFVKA 193

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BLAST of CaUC02G041450 vs. NCBI nr
Match: KAG7030262.1 (hypothetical protein SDJN02_08609, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 335.9 bits (860), Expect = 2.4e-88
Identity = 160/178 (89.89%), Postives = 168/178 (94.38%), Query Frame = 0


Query: 18  MAITSTAQLPPVLDTDGEPLRRGVEYYIKPAITDVAGNLTLKSRSNAPCPLYVGQEAVTS 77
           MAITSTAQLPPVLDTDG+PLRRGVEYYIKPAITDVAGNLTLKSRSNAPCPLYVGQE + S
Sbjct: 1   MAITSTAQLPPVLDTDGQPLRRGVEYYIKPAITDVAGNLTLKSRSNAPCPLYVGQEPIAS 60

Query: 78  TNIGLPVTFTPIVASEDIIEESRGLNIVFQASSTCIMSTQWRVDEAESDTGRRFVGTGND 137
           TNIGLPVTFTPIVA EDIIEES G N+VFQASSTCI STQWRVDE ES TGRRFVG GND
Sbjct: 61  TNIGLPVTFTPIVAGEDIIEESMGFNVVFQASSTCITSTQWRVDEMESATGRRFVGVGND 120

Query: 138 DGPTGVFRIDRNNGVYNIVWCPAMMGRPRCGSAGILVEDGVRLLALDGDAFPFEFVKS 196
           +GPTG+FRIDRNNGVYNIVWCP M+GRPRCGSAGILVEDGVRLLALDG+AFPFEFVK+
Sbjct: 121 NGPTGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVEDGVRLLALDGNAFPFEFVKA 178

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BLAST of CaUC02G041450 vs. ExPASy Swiss-Prot
Match: G7LCV1 (Kunitz type trypsin inhibitor 106 OS=Medicago truncatula OX=3880 GN=KPI106 PE=1 SV=1)

HSP 1 Score: 142.5 bits (358), Expect = 5.0e-33
Identity = 88/205 (42.93%), Postives = 118/205 (57.56%), Query Frame = 0


Query: 1   MMMLHKSLAILSYVCLFMAITSTAQLPPVLDTDGEPLRRGVEYYIKPAITDVAGNLTLKS 60
           M +  ++L IL++VCLF+  T+ AQ   VLDT GEP+    EY+I+P IT+  G  T+ S
Sbjct: 3   MRLSIRTLIILAHVCLFITTTTIAQF--VLDTVGEPVEGDEEYFIRPVITNKGGRSTMVS 62

Query: 61  RSNAPCPLYVGQEAVTSTNIGLPVTFTPIVASEDI--IEESRGLNIVFQASSTCIMSTQW 120
           R N  CPL+VG E +T    GL V FTP     D   +  +R L I FQASS+C+ ST+W
Sbjct: 63  R-NESCPLHVGLE-LTGLGRGLVVKFTPFAPHHDFDDVRVNRDLRITFQASSSCVQSTEW 122

Query: 121 RVDEAESDTGRRFVGTGNDDGPTG----VFRIDRN--NGVYNIVWCPAMM---GRPRCGS 180
           R+ E ++ +GRR + TG D    G     FRI      G+YNI WCP  +    +  CG+
Sbjct: 123 RLGEKDTKSGRRLIITGTDSATNGSYGNFFRIVETPLEGMYNIQWCPTEVCPSCKFECGT 182

Query: 181 AGILVEDGVRLLALDGDAFPFEFVK 195
             +L E+G  LLALDG   P  F K
Sbjct: 183 VDMLNENGKILLALDGGPLPLVFQK 203

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BLAST of CaUC02G041450 vs. ExPASy Swiss-Prot
Match: A0A072TH68 (Kunitz type trypsin inhibitor 104 OS=Medicago truncatula OX=3880 GN=KPI104 PE=1 SV=1)

HSP 1 Score: 139.8 bits (351), Expect = 3.2e-32
Identity = 83/198 (41.92%), Postives = 115/198 (58.08%), Query Frame = 0


Query: 7   SLAILSYVCLFMAITSTAQLPPVLDTDGEPLRRGVEYYIKPAITDVAGNLTLKSRSNAPC 66
           ++ IL++V L MA TS AQ   V+DT GEP+    EY+I+PAIT   G  TL +  N PC
Sbjct: 7   TIFILAHVWLLMATTSIAQF--VIDTSGEPVEDDEEYFIRPAITGNGGGSTLVT-GNGPC 66

Query: 67  PLYVGQEAVTSTNIGLPVTFTPIVA--SEDIIEESRGLNIVFQASSTCIMSTQWRVDEAE 126
           PL+VG +    T +G+ V FTP      +D +  +R L + F  S++C  ST WR+ E +
Sbjct: 67  PLHVGLDNTEGT-LGVAVKFTPFAPQHDDDDVRLNRDLRVTFLTSTSCGQSTDWRLGEKD 126

Query: 127 SDTGRRFVGTGNDDG---PTGVFRI--DRNNGVYNIVWCP---AMMGRPRCGSAGILVED 186
           + +GRR + TG D+G       FRI   +  G YNI WCP       + +CG+ G++ E+
Sbjct: 127 ATSGRRLIVTGRDNGAGSQGNFFRIVQTQTGGTYNIQWCPTEACPSCKVQCGTVGVIREN 186

Query: 187 GVRLLALDGDAFPFEFVK 195
           G  LLALDGDA P  F K
Sbjct: 187 GKNLLALDGDALPVVFQK 200

[Close]

BLAST of CaUC02G041450 vs. ExPASy Swiss-Prot
Match: G7LCV7 (Kunitz type trypsin inhibitor 111 OS=Medicago truncatula OX=3880 GN=KPI111 PE=1 SV=1)

HSP 1 Score: 132.5 bits (332), Expect = 5.2e-30
Identity = 82/205 (40.00%), Postives = 119/205 (58.05%), Query Frame = 0


Query: 1   MMMLHKSLAILSYVCLFMAITSTAQLPPVLDTDGEPLRRGVEYYIKPAITDVAGNLTLKS 60
           M  +  ++ IL+ V L +  TS AQ   V+DT GEP+    +Y+I+PAIT   G+LTL +
Sbjct: 1   MSTISFTIFILANVWLLVVTTSIAQF--VIDTSGEPVENDEDYFIRPAITGNGGSLTLVT 60

Query: 61  RSNAPCPLYVGQEAVTSTNIGLPVTFTPIVAS--EDIIEESRGLNIVFQASSTCIMSTQW 120
           R++  CP  VG +       G  V  +P V++  ED +   R L ++FQA ++C  ST+W
Sbjct: 61  RNS--CPFNVGLD--PDAPQGFAVLLSPFVSNREEDEVRLGRDLRVIFQAGTSCGQSTEW 120

Query: 121 RVDEAESDTGRRFVGTGNDDGPTG----VFRIDR--NNGVYNIVWCPAMM---GRPRCGS 180
           R+ E ++ TGRRF+ TG DD   G     FRI +  + G++NI WCP  +    +  CG+
Sbjct: 121 RLGERDATTGRRFIITGRDDSTVGSYGNFFRIVQTPSRGIFNIQWCPTEVCPSCKFECGT 180

Query: 181 AGILVEDGVRLLALDGDAFPFEFVK 195
            GI+ E+G  LLALDG A P  F K
Sbjct: 181 VGIVRENGKILLALDGSALPVAFQK 199

[Close]

BLAST of CaUC02G041450 vs. ExPASy Swiss-Prot
Match: P13087 (Miraculin OS=Synsepalum dulcificum OX=3743 PE=1 SV=3)

HSP 1 Score: 100.9 bits (250), Expect = 1.7e-20
Identity = 68/184 (36.96%), Postives = 92/184 (50.00%), Query Frame = 0


Query: 28  PVLDTDGEPLRRGVEYYIKPAITDVAGNLTLKS---RSNAPCPLYVGQEAVTSTNIGLPV 87
           PVLD DGE LR G  YYI P + D  G LT+ +        CP  V Q      +   P+
Sbjct: 35  PVLDIDGEKLRTGTNYYIVPVLRDHGGGLTVSATTPNGTFVCPPRVVQTR-KEVDHDRPL 94

Query: 88  TFTPIVASEDIIEESRGLNIVFQASSTC--IMSTQWRVDEAESDTGRRFV---GTGNDDG 147
            F P    ED++  S  LNI F A   C    ST WR+D+ +  TG+ FV   G   + G
Sbjct: 95  AFFPENPKEDVVRVSTDLNINFSAFMPCRWTSSTVWRLDKYDESTGQYFVTIGGVKGNPG 154

Query: 148 PTGV---FRIDR--NNGVYNIVWCPAMMG--RPRCGSAGILVED-GVRLLALDGDAFPFE 196
           P  +   F+I+    +G Y +V+CP + G  + +CG  GI ++  G R LAL    F FE
Sbjct: 155 PETISSWFKIEEFCGSGFYKLVFCPTVCGSCKVKCGDVGIYIDQKGRRRLALSDKPFAFE 214

[Close]

BLAST of CaUC02G041450 vs. ExPASy Swiss-Prot
Match: P16347 (Endogenous alpha-amylase/subtilisin inhibitor OS=Triticum aestivum OX=4565 PE=1 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 4.3e-16
Identity = 57/143 (39.86%), Postives = 75/143 (52.45%), Query Frame = 0


Query: 27  PPVLDTDGEPLRRGVEYYIKPAITDVAGNLTLKSRSNAPCPLYVGQEAVTSTNIGLPVTF 86
           PPV DTDG  LR    YY+ PA     G LT+       CPL+V QEA      GLPV  
Sbjct: 3   PPVHDTDGNELRADANYYVLPANRAHGGGLTMAPGHGRRCPLFVSQEA-DGQRDGLPVRI 62

Query: 87  TPI--VASEDIIEESRGLNIVFQASSTCIMSTQWRVDEAESDTGRRFVGTG--NDDGPTG 146
            P     S+ II  S  + I F+A +TC+ ST+W +D +E  +GRR V TG   D  P+G
Sbjct: 63  APHGGAPSDKIIRLSTDVRISFRAYTTCVQSTEWHID-SELVSGRRHVITGPVRDPSPSG 122

Query: 147 ---VFRIDRNNGV----YNIVWC 159
               FRI++ +G     Y ++ C
Sbjct: 123 RENAFRIEKYSGAEVHEYKLMAC 143

[Close]

BLAST of CaUC02G041450 vs. ExPASy TrEMBL
Match: A0A6J1G3G3 (miraculin-like OS=Cucurbita moschata OX=3662 GN=LOC111450419 PE=3 SV=1)

HSP 1 Score: 351.7 bits (901), Expect = 2.0e-93
Identity = 169/193 (87.56%), Postives = 180/193 (93.26%), Query Frame = 0


Query: 3   MLHKSLAILSYVCLFMAITSTAQLPPVLDTDGEPLRRGVEYYIKPAITDVAGNLTLKSRS 62
           MLH SLAI S++ +FMAITSTAQLPPVLDTDG+PLRRGVEYYIKPAITDVAGNLTLKSRS
Sbjct: 1   MLHNSLAIFSFLLVFMAITSTAQLPPVLDTDGQPLRRGVEYYIKPAITDVAGNLTLKSRS 60

Query: 63  NAPCPLYVGQEAVTSTNIGLPVTFTPIVASEDIIEESRGLNIVFQASSTCIMSTQWRVDE 122
           NAPCPLYVGQE + STNIGLPVTFTPIVA EDIIEES G N+VFQASSTCI STQWRVDE
Sbjct: 61  NAPCPLYVGQEPIASTNIGLPVTFTPIVAGEDIIEESMGFNVVFQASSTCITSTQWRVDE 120

Query: 123 AESDTGRRFVGTGNDDGPTGVFRIDRNNGVYNIVWCPAMMGRPRCGSAGILVEDGVRLLA 182
            ES TGRRFVG GND+GPTG+FRIDRNNGVYNIVWCP M+GRPRCGSAGILVEDGVRLLA
Sbjct: 121 MESATGRRFVGVGNDNGPTGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVEDGVRLLA 180

Query: 183 LDGDAFPFEFVKS 196
           LDG+AFPFEFVK+
Sbjct: 181 LDGNAFPFEFVKA 193

[Close]

BLAST of CaUC02G041450 vs. ExPASy TrEMBL
Match: A0A6J1KC85 (miraculin-like OS=Cucurbita maxima OX=3661 GN=LOC111493610 PE=3 SV=1)

HSP 1 Score: 347.8 bits (891), Expect = 2.9e-92
Identity = 167/193 (86.53%), Postives = 179/193 (92.75%), Query Frame = 0


Query: 3   MLHKSLAILSYVCLFMAITSTAQLPPVLDTDGEPLRRGVEYYIKPAITDVAGNLTLKSRS 62
           MLH SLAI SY+ +F+AITSTAQLPPVLDTDG+PLRRGVEYYIKPAIT+VAGNLTLK+RS
Sbjct: 1   MLHNSLAIFSYLLVFLAITSTAQLPPVLDTDGQPLRRGVEYYIKPAITNVAGNLTLKNRS 60

Query: 63  NAPCPLYVGQEAVTSTNIGLPVTFTPIVASEDIIEESRGLNIVFQASSTCIMSTQWRVDE 122
           NAPCPLYVGQE V STNIGLPVTFTPIVA EDIIEES G N+ FQASSTCI STQWRVDE
Sbjct: 61  NAPCPLYVGQEPVASTNIGLPVTFTPIVAGEDIIEESMGFNVAFQASSTCITSTQWRVDE 120

Query: 123 AESDTGRRFVGTGNDDGPTGVFRIDRNNGVYNIVWCPAMMGRPRCGSAGILVEDGVRLLA 182
            ES TGRRFVG GND+GPTG+FRIDRNNGVYNIVWCP M+GRPRCGSAGILVEDGVRLLA
Sbjct: 121 MESATGRRFVGVGNDNGPTGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVEDGVRLLA 180

Query: 183 LDGDAFPFEFVKS 196
           LDG+AFPFEFVK+
Sbjct: 181 LDGNAFPFEFVKA 193

[Close]

BLAST of CaUC02G041450 vs. ExPASy TrEMBL
Match: A0A5D3D468 (Miraculin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G00520 PE=3 SV=1)

HSP 1 Score: 326.6 bits (836), Expect = 6.9e-86
Identity = 156/195 (80.00%), Postives = 170/195 (87.18%), Query Frame = 0


Query: 1   MMMLHKSLAILSYVCLFMAITSTAQLPPVLDTDGEPLRRGVEYYIKPAITDVAGNLTLKS 60
           MMMLHKSLAI  Y CLFMAITSTAQ PPVLDT+G+PLRRGVEYYI PAITDV GNLTLKS
Sbjct: 1   MMMLHKSLAIFGYACLFMAITSTAQSPPVLDTNGQPLRRGVEYYISPAITDVGGNLTLKS 60

Query: 61  RSNAPCPLYVGQEAVTSTNIGLPVTFTPIVASEDIIEESRGLNIVFQASSTCIMSTQWRV 120
           RSNAPCPL+VGQE VTSTNIGLPVTF P  A +DII+E   LNIVF+A STC  STQWRV
Sbjct: 61  RSNAPCPLFVGQEPVTSTNIGLPVTFRPTEAGKDIIDEGTSLNIVFEALSTCATSTQWRV 120

Query: 121 DEAESDTGRRFVGTGNDDGPTGVFRIDRNNGVYNIVWCPAMMGRPRCGSAGILVEDGVRL 180
           D  ESDTGRRFVG G++DGP G+F I R+NG YNIVWCPAMMGRPRCG AGILVE+GVRL
Sbjct: 121 DATESDTGRRFVGIGDEDGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRL 180

Query: 181 LALDGDAFPFEFVKS 196
           +ALDGDAFPFEF+K+
Sbjct: 181 VALDGDAFPFEFIKA 195

[Close]

BLAST of CaUC02G041450 vs. ExPASy TrEMBL
Match: A0A5A7VDV8 (Miraculin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G00530 PE=3 SV=1)

HSP 1 Score: 323.6 bits (828), Expect = 5.9e-85
Identity = 154/193 (79.79%), Postives = 168/193 (87.05%), Query Frame = 0


Query: 3   MLHKSLAILSYVCLFMAITSTAQLPPVLDTDGEPLRRGVEYYIKPAITDVAGNLTLKSRS 62
           MLHKSLAI  Y CLFMAITSTAQ PPVLDT+G+PLRRGVEYYI PAITDV GNLTLKSRS
Sbjct: 1   MLHKSLAIFGYACLFMAITSTAQSPPVLDTNGQPLRRGVEYYISPAITDVGGNLTLKSRS 60

Query: 63  NAPCPLYVGQEAVTSTNIGLPVTFTPIVASEDIIEESRGLNIVFQASSTCIMSTQWRVDE 122
           NAPCPL+VGQE VTSTNIGLPVTF P  A +DII+E   LNIVF+A STC  STQWRVD 
Sbjct: 61  NAPCPLFVGQEPVTSTNIGLPVTFRPTEAGKDIIDEGTSLNIVFEALSTCATSTQWRVDA 120

Query: 123 AESDTGRRFVGTGNDDGPTGVFRIDRNNGVYNIVWCPAMMGRPRCGSAGILVEDGVRLLA 182
            ESDTGRRFVG G++DGP G+F I R+NG YNIVWCPAMMGRPRCG AGILVE+GVRL+A
Sbjct: 121 TESDTGRRFVGIGDEDGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRLVA 180

Query: 183 LDGDAFPFEFVKS 196
           LDGDAFPFEF+K+
Sbjct: 181 LDGDAFPFEFIKA 193

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BLAST of CaUC02G041450 vs. ExPASy TrEMBL
Match: A0A6J1DB43 (miraculin-like OS=Momordica charantia OX=3673 GN=LOC111018852 PE=3 SV=1)

HSP 1 Score: 302.4 bits (773), Expect = 1.4e-78
Identity = 154/197 (78.17%), Postives = 167/197 (84.77%), Query Frame = 0


Query: 2   MMLHKSLAILSYVCL-FMAI-TSTAQLPPVLDTDGEPLRRGVEYYIKPAITDVAGNLTLK 61
           M LHKSLAILS +CL FMAI +STAQ PPVLDT+G+ LRRG+EYYIKPAITDVAGNLTLK
Sbjct: 1   MTLHKSLAILSSLCLIFMAISSSTAQPPPVLDTNGQALRRGIEYYIKPAITDVAGNLTLK 60

Query: 62  SRSNAPCPLYVGQEAVTSTNIGLPVTFTPIVASEDIIEESRGLNIVFQASSTCIMSTQWR 121
           SRS APCPLYVGQE V S  IGLPV FTP  A EDII E     +VFQASSTCIM TQWR
Sbjct: 61  SRSRAPCPLYVGQEPVASMEIGLPVIFTPYAADEDIIREGMDFKVVFQASSTCIMLTQWR 120

Query: 122 VDEAESDTGRRFVGTGNDDGPTGVFRIDRNN-GVYNIVWCPAMMGRPRCGSAGILVEDGV 181
           V+E E+ TGRR VGTG+D+GPTG+FRIDR+N GVYNI WCP M GRPRCGSAGILVEDGV
Sbjct: 121 VEETEAATGRRLVGTGDDNGPTGMFRIDRSNLGVYNIGWCPEMFGRPRCGSAGILVEDGV 180

Query: 182 RLLALDGDAFPFEFVKS 196
           RLLALDG AFPFEFVK+
Sbjct: 181 RLLALDGSAFPFEFVKA 197

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BLAST of CaUC02G041450 vs. TAIR 10
Match: AT1G73325.1 (Kunitz family trypsin and protease inhibitor protein )

HSP 1 Score: 70.9 bits (172), Expect = 1.3e-12
Identity = 56/196 (28.57%), Postives = 93/196 (47.45%), Query Frame = 0


Query: 7   SLAILSYVCLFMAITSTAQLPPVLDTDGEPLRRGVEYYIKPAITDVAGNLTLKSRSNAPC 66
           +L +LS +    +         VLD  G P++  V+YYI PA     G L   +R+ +  
Sbjct: 10  TLTVLSAIFTAASAADATPSQVVLDIAGHPVQSNVQYYIIPAKIGTGGGLIPSNRNLSTQ 69

Query: 67  PLYVGQEAVTSTN---IGLPVTFTPIVASEDIIEESRGLNIVFQASS-TCIMSTQWRVDE 126
            L +  + V S++    GLPVTF+P+      ++ S  LN+ F ++   C  S  WR+D 
Sbjct: 70  DLCLNLDIVQSSSPFVSGLPVTFSPLNTKVKHVQLSASLNLEFDSTVWLCPDSKVWRIDH 129

Query: 127 AESDTGRRFVGTGNDDGP-TGVFRIDRNNGVYNIVWCPAMMGRPRCGSAGILVED-GVRL 186
           +     + FV  G   G     F+I  +   Y +++CP +     C +  + ++D GVR 
Sbjct: 130 S-VQLRKSFVSIGGQKGKGNSWFQIQEDGDAYKLMYCP-ISSIVACINVSLEIDDHGVRR 189

Query: 187 LALDGD-AFPFEFVKS 196
           L L  D +F  +F K+
Sbjct: 190 LVLSTDQSFVVKFQKA 203

The following BLAST results are available for this feature:

BLAST of CaUC02G041450 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038888526.1	4.7e-97	90.26	kunitz type trypsin inhibitor 106-like [Benincasa hispida]	[more]
XP_022946294.1	4.1e-93	87.56	miraculin-like [Cucurbita moschata] >XP_022946295.1 miraculin-like [Cucurbita mo...	[more]
XP_023545477.1	4.6e-92	87.05	miraculin-like [Cucurbita pepo subsp. pepo]	[more]
XP_022999136.1	6.0e-92	86.53	miraculin-like [Cucurbita maxima]	[more]
KAG7030262.1	2.4e-88	89.89	hypothetical protein SDJN02_08609, partial [Cucurbita argyrosperma subsp. argyro...	[more]

BLAST of CaUC02G041450 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
G7LCV1	5.0e-33	42.93	Kunitz type trypsin inhibitor 106 OS=Medicago truncatula OX=3880 GN=KPI106 PE=1 ...	[more]
A0A072TH68	3.2e-32	41.92	Kunitz type trypsin inhibitor 104 OS=Medicago truncatula OX=3880 GN=KPI104 PE=1 ...	[more]
G7LCV7	5.2e-30	40.00	Kunitz type trypsin inhibitor 111 OS=Medicago truncatula OX=3880 GN=KPI111 PE=1 ...	[more]
P13087	1.7e-20	36.96	Miraculin OS=Synsepalum dulcificum OX=3743 PE=1 SV=3	[more]
P16347	4.3e-16	39.86	Endogenous alpha-amylase/subtilisin inhibitor OS=Triticum aestivum OX=4565 PE=1 ...	[more]

BLAST of CaUC02G041450 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G3G3	2.0e-93	87.56	miraculin-like OS=Cucurbita moschata OX=3662 GN=LOC111450419 PE=3 SV=1	[more]
A0A6J1KC85	2.9e-92	86.53	miraculin-like OS=Cucurbita maxima OX=3661 GN=LOC111493610 PE=3 SV=1	[more]
A0A5D3D468	6.9e-86	80.00	Miraculin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G00520...	[more]
A0A5A7VDV8	5.9e-85	79.79	Miraculin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G00530...	[more]
A0A6J1DB43	1.4e-78	78.17	miraculin-like OS=Momordica charantia OX=3673 GN=LOC111018852 PE=3 SV=1	[more]

BLAST of CaUC02G041450 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G73325.1	1.3e-12	28.57	Kunitz family trypsin and protease inhibitor protein	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (USVL246-FR2) v1
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002160	Proteinase inhibitor I3, Kunitz legume	SMART	SM00452	kul_2	coord: 28..195 e-value: 4.5E-32 score: 122.5
IPR002160	Proteinase inhibitor I3, Kunitz legume	PFAM	PF00197	Kunitz_legume	coord: 29..194 e-value: 4.8E-43 score: 147.1
IPR002160	Proteinase inhibitor I3, Kunitz legume	PANTHER	PTHR33107	KUNITZ TRYPSIN INHIBITOR 2	coord: 5..194
IPR002160	Proteinase inhibitor I3, Kunitz legume	PROSITE	PS00283	SOYBEAN_KUNITZ	coord: 29..45
IPR002160	Proteinase inhibitor I3, Kunitz legume	CDD	cd00178	STI	coord: 28..194 e-value: 6.9716E-42 score: 137.088
None	No IPR available	GENE3D	2.80.10.50		coord: 24..195 e-value: 1.6E-47 score: 163.4
None	No IPR available	PANTHER	PTHR33107:SF51	PUTATIVE-RELATED	coord: 5..194
IPR011065	Kunitz inhibitor STI-like superfamily	SUPERFAMILY	50386	STI-like	coord: 27..194

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CaUC02G041450.1	CaUC02G041450.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006952	defense response
biological_process	GO:0010951	negative regulation of endopeptidase activity
biological_process	GO:0007165	signal transduction
molecular_function	GO:0043531	ADP binding
molecular_function	GO:0004866	endopeptidase inhibitor activity
molecular_function	GO:0003953	NAD+ nucleosidase activity