CaUC02G032330 (gene) Watermelon (USVL246-FR2) v1

Overview


Name	CaUC02G032330
Type	gene
Organism	Citrullus amarus (Watermelon (USVL246-FR2) v1)
Description	Malic enzyme
Location	Ciama_Chr02: 7088559 .. 7089509 (-)
RNA-Seq Expression	CaUC02G032330
Synteny	CaUC02G032330

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTATGGTCTAGTTGTTAGTTCTTGTAAGGAATCACTCCAACTTCTCAAGCAACCTTGGGCACACAAGCATGAACCAATTAAGGATCATGTTGGTGTTGTGAAGGTCTTTTTCTTAACATCTCCATTTAAAATTTGCATACGAGTAAAAAGACAAAGGTTTCATAGTTTTTATCTTCCTATCTCATTTCAAGATATCAAACCAACAGTGTTGATTGGGACATCAGGCATGGGAAGGACATTTACTAAAGAGGTTATGGAGGCCATAGCCGCCATTAACGAGGTTTTTTATTTGCAAAACTAGCTCACTTGCATTTTGTGTAGATTCAAATGAGAAAATATATTGACAGTAATTTTATTATAGATTGCAGAAACCAATAATTCTTGCTCTTTTGAACCCCACTTCTCAATTTGAATGCACTGCTGAAGAGGCTTATACATGGACTGAGGTACTACCTACATGCACAAAATACCATGTCCTAGTTGTTTTTGTCGATGTTAACTCAATACTTCTATTTTGACTTCATTTTATATGGTAATTTTTCCCTTTCCAGGGTCGTGCCATTTTTGCTAGTGGTGGCCCATTTGATCCTGTTGAATACAAAAGGAATGTCTTTGTATCTAAGCAGGTCTTATACATACATACCCTATGCCTAACTTCCATTTTCTCTTTTACCCTCAACTGTCTTTCATTAAAATGATAAGTTTCACGTTATTACTATTTTCCTTTATTACTTAGGCAAATAAGGCTTATATTTTTCTTGGCTTTCGTCTTGGATTAATCATGTTCGGTACCATTCGCATTCGTGATGATATGCTCCTAGCAGCCTGCAAGTGCAAAAAAGACTTGGACTTTGAGTTTGATACCCAATTCAAAATTATCAATATTTATTTATTTATTTATTTATTTCTACATTTCAGTGCCCTCGTCAAACAGGTCGTTCAGAAGTGA

mRNA sequence

ATGTATGGTCTAGTTGTTAGTTCTTGTAAGGAATCACTCCAACTTCTCAAGCAACCTTGGGCACACAAGCATGAACCAATTAAGGATCATGTTGGTGTTGTGAAGGTCTTTTTCTTAACATCTCCATTTAAAATTTGCATACGAGTAAAAAGACAAAGGTTTCATAGTTTTTATCTTCCTATCTCATTTCAAGATATCAAACCAACAGTGTTGATTGGGACATCAGGCATGGGAAGGACATTTACTAAAGAGGTTATGGAGGCCATAGCCGCCATTAACGAGAAACCAATAATTCTTGCTCTTTTGAACCCCACTTCTCAATTTGAATGCACTGCTGAAGAGGCTTATACATGGACTGAGGGTCGTGCCATTTTTGCTAGTGGTGGCCCATTTGATCCTGTTGAATACAAAAGGAATGTCTTTGTATCTAAGCAGGCAAATAAGGCTTATATTTTTCTTGGCTTTCGTCTTGGATTAATCATGTTCGGTACCATTCGCATTCGTGATGATATGCTCCTAGCAGCCTGCAAGTGCAAAAAAGACTTGGACTTTGAGTTTGATACCCAATTCAAAATTATCAATATTTATTTATTTATTTATTTATTTCTACATTTCAGTGCCCTCGTCAAACAGGTCGTTCAGAAGTGA

Coding sequence (CDS)

ATGTATGGTCTAGTTGTTAGTTCTTGTAAGGAATCACTCCAACTTCTCAAGCAACCTTGGGCACACAAGCATGAACCAATTAAGGATCATGTTGGTGTTGTGAAGGTCTTTTTCTTAACATCTCCATTTAAAATTTGCATACGAGTAAAAAGACAAAGGTTTCATAGTTTTTATCTTCCTATCTCATTTCAAGATATCAAACCAACAGTGTTGATTGGGACATCAGGCATGGGAAGGACATTTACTAAAGAGGTTATGGAGGCCATAGCCGCCATTAACGAGAAACCAATAATTCTTGCTCTTTTGAACCCCACTTCTCAATTTGAATGCACTGCTGAAGAGGCTTATACATGGACTGAGGGTCGTGCCATTTTTGCTAGTGGTGGCCCATTTGATCCTGTTGAATACAAAAGGAATGTCTTTGTATCTAAGCAGGCAAATAAGGCTTATATTTTTCTTGGCTTTCGTCTTGGATTAATCATGTTCGGTACCATTCGCATTCGTGATGATATGCTCCTAGCAGCCTGCAAGTGCAAAAAAGACTTGGACTTTGAGTTTGATACCCAATTCAAAATTATCAATATTTATTTATTTATTTATTTATTTCTACATTTCAGTGCCCTCGTCAAACAGGTCGTTCAGAAGTGA

Protein sequence

MYGLVVSSCKESLQLLKQPWAHKHEPIKDHVGVVKVFFLTSPFKICIRVKRQRFHSFYLPISFQDIKPTVLIGTSGMGRTFTKEVMEAIAAINEKPIILALLNPTSQFECTAEEAYTWTEGRAIFASGGPFDPVEYKRNVFVSKQANKAYIFLGFRLGLIMFGTIRIRDDMLLAACKCKKDLDFEFDTQFKIINIYLFIYLFLHFSALVKQVVQK

Homology

[Close]

BLAST of CaUC02G032330 vs. NCBI nr
Match: XP_023547383.1 (NADP-dependent malic enzyme-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023547802.1 NADP-dependent malic enzyme-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 252.3 bits (643), Expect = 3.8e-63
Identity = 129/173 (74.57%), Postives = 142/173 (82.08%), Query Frame = 0


Query: 3   GLVVSSCKESLQLLKQPWAHKHEPIKDHVGVVKVFFLTSPFKICIRVKRQRFHSFYLPIS 62
           GL+VSS KESLQ  K+PWAH+HEPI+D V  VKV FL SPFK   +  +       +PIS
Sbjct: 416 GLIVSSRKESLQHFKKPWAHEHEPIQDLVDAVKVSFLPSPFKFLHKPMK-------VPIS 475

Query: 63  FQDIKPTVLIGTSGMGRTFTKEVMEAIAAINEKPIILALLNPTSQFECTAEEAYTWTEGR 122
           FQDIKPTVLIGTSG+GRTFTK+V+EA+AAIN+KPIILAL NPTSQ ECTAEEAYTWTEGR
Sbjct: 476 FQDIKPTVLIGTSGVGRTFTKDVVEAMAAINKKPIILALSNPTSQSECTAEEAYTWTEGR 535

Query: 123 AIFASGGPFDPVEYKRNVFVSKQANKAYIFLGFRLGLIMFGTIRIRDDMLLAA 176
           AIFASG PFDPVEY  NVFV  QAN AYIF GF LGLIM GTIR+RDDMLLAA
Sbjct: 536 AIFASGSPFDPVEYNGNVFVPGQANNAYIFPGFGLGLIMSGTIRVRDDMLLAA 581

[Close]

BLAST of CaUC02G032330 vs. NCBI nr
Match: XP_022953374.1 (NADP-dependent malic enzyme-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 252.3 bits (643), Expect = 3.8e-63
Identity = 129/173 (74.57%), Postives = 142/173 (82.08%), Query Frame = 0


Query: 3   GLVVSSCKESLQLLKQPWAHKHEPIKDHVGVVKVFFLTSPFKICIRVKRQRFHSFYLPIS 62
           GL+VSS KESLQ  K+PWAH+HEPI+D V  VKV FL SPFK   +  +       +PIS
Sbjct: 416 GLIVSSRKESLQHFKKPWAHEHEPIQDLVDAVKVSFLPSPFKFLHKPMK-------VPIS 475

Query: 63  FQDIKPTVLIGTSGMGRTFTKEVMEAIAAINEKPIILALLNPTSQFECTAEEAYTWTEGR 122
           FQDIKPTVLIGTSG+GRTFTK+V+EA+AAIN+KPIILAL NPTSQ ECTAEEAYTWTEGR
Sbjct: 476 FQDIKPTVLIGTSGVGRTFTKDVVEAMAAINKKPIILALSNPTSQSECTAEEAYTWTEGR 535

Query: 123 AIFASGGPFDPVEYKRNVFVSKQANKAYIFLGFRLGLIMFGTIRIRDDMLLAA 176
           AIFASG PFDPVEY  NVFV  QAN AYIF GF LGLIM GTIR+RDDMLLAA
Sbjct: 536 AIFASGSPFDPVEYNGNVFVPGQANNAYIFPGFGLGLIMSGTIRVRDDMLLAA 581

[Close]

BLAST of CaUC02G032330 vs. NCBI nr
Match: XP_008462428.1 (PREDICTED: NADP-dependent malic enzyme 4, chloroplastic-like isoform X2 [Cucumis melo] >TYK03837.1 NADP-dependent malic enzyme 4 [Cucumis melo var. makuwa])

HSP 1 Score: 224.9 bits (572), Expect = 6.5e-55
Identity = 118/173 (68.21%), Postives = 128/173 (73.99%), Query Frame = 0


Query: 3   GLVVSSCKESLQLLKQPWAHKHEPIKDHVGVVKVFFLTSPFKICIRVKRQRFHSFYLPIS 62
           GL+VSS KESLQ  K+PWAH+HEPIKD +  VK                           
Sbjct: 353 GLIVSSRKESLQHFKKPWAHEHEPIKDLIDAVK--------------------------- 412

Query: 63  FQDIKPTVLIGTSGMGRTFTKEVMEAIAAINEKPIILALLNPTSQFECTAEEAYTWTEGR 122
             DIKPTVLIGTSG+GRTFTK+V+EA+AAINEKPIILAL NPTSQ ECTAEEAYTWTEGR
Sbjct: 413 --DIKPTVLIGTSGVGRTFTKDVVEAMAAINEKPIILALSNPTSQSECTAEEAYTWTEGR 472

Query: 123 AIFASGGPFDPVEYKRNVFVSKQANKAYIFLGFRLGLIMFGTIRIRDDMLLAA 176
           AIFASG PFDPVEY+ NVFV  QAN AYIF GF LGLIM GTIR+RDDMLLAA
Sbjct: 473 AIFASGSPFDPVEYEGNVFVPGQANNAYIFPGFGLGLIMSGTIRVRDDMLLAA 496

[Close]

BLAST of CaUC02G032330 vs. NCBI nr
Match: XP_038900186.1 (NADP-dependent malic enzyme-like isoform X1 [Benincasa hispida])

HSP 1 Score: 224.6 bits (571), Expect = 8.4e-55
Identity = 118/173 (68.21%), Postives = 128/173 (73.99%), Query Frame = 0


Query: 3   GLVVSSCKESLQLLKQPWAHKHEPIKDHVGVVKVFFLTSPFKICIRVKRQRFHSFYLPIS 62
           GL+VSS KESLQ  K+PWAH+HEPIKD + VVK                           
Sbjct: 374 GLIVSSRKESLQHFKKPWAHEHEPIKDLLDVVK--------------------------- 433

Query: 63  FQDIKPTVLIGTSGMGRTFTKEVMEAIAAINEKPIILALLNPTSQFECTAEEAYTWTEGR 122
             DIKPT LIGTSG+G+TFTK+V+EA+AAINEKPIILAL NPTSQ ECTAEEAYTWTEGR
Sbjct: 434 --DIKPTALIGTSGVGKTFTKDVVEAMAAINEKPIILALSNPTSQSECTAEEAYTWTEGR 493

Query: 123 AIFASGGPFDPVEYKRNVFVSKQANKAYIFLGFRLGLIMFGTIRIRDDMLLAA 176
           AIFASG PFDPVEYK NVFV  QAN AYIF GF LGLIM GTIR+RDDMLLAA
Sbjct: 494 AIFASGSPFDPVEYKGNVFVPGQANNAYIFPGFGLGLIMSGTIRVRDDMLLAA 517

[Close]

BLAST of CaUC02G032330 vs. NCBI nr
Match: XP_038900188.1 (NADP-dependent malic enzyme 4, chloroplastic-like isoform X2 [Benincasa hispida])

HSP 1 Score: 224.6 bits (571), Expect = 8.4e-55
Identity = 118/173 (68.21%), Postives = 128/173 (73.99%), Query Frame = 0


Query: 3   GLVVSSCKESLQLLKQPWAHKHEPIKDHVGVVKVFFLTSPFKICIRVKRQRFHSFYLPIS 62
           GL+VSS KESLQ  K+PWAH+HEPIKD + VVK                           
Sbjct: 358 GLIVSSRKESLQHFKKPWAHEHEPIKDLLDVVK--------------------------- 417

Query: 63  FQDIKPTVLIGTSGMGRTFTKEVMEAIAAINEKPIILALLNPTSQFECTAEEAYTWTEGR 122
             DIKPT LIGTSG+G+TFTK+V+EA+AAINEKPIILAL NPTSQ ECTAEEAYTWTEGR
Sbjct: 418 --DIKPTALIGTSGVGKTFTKDVVEAMAAINEKPIILALSNPTSQSECTAEEAYTWTEGR 477

Query: 123 AIFASGGPFDPVEYKRNVFVSKQANKAYIFLGFRLGLIMFGTIRIRDDMLLAA 176
           AIFASG PFDPVEYK NVFV  QAN AYIF GF LGLIM GTIR+RDDMLLAA
Sbjct: 478 AIFASGSPFDPVEYKGNVFVPGQANNAYIFPGFGLGLIMSGTIRVRDDMLLAA 501

[Close]

BLAST of CaUC02G032330 vs. ExPASy Swiss-Prot
Match: O82191 (NADP-dependent malic enzyme 1 OS=Arabidopsis thaliana OX=3702 GN=NADP-ME1 PE=1 SV=1)

HSP 1 Score: 204.1 bits (518), Expect = 1.5e-51
Identity = 101/175 (57.71%), Postives = 127/175 (72.57%), Query Frame = 0


Query: 3   GLVVSSCKESLQLLKQPWAHKHEPIKDHVGVVKVFFLTSPFKICIRVKRQRFHSFYLPIS 62
           GL+V+S K+SLQ  K+PWAH+HEP+KD +G +K                           
Sbjct: 366 GLIVNSRKDSLQDFKKPWAHEHEPVKDLLGAIKA-------------------------- 425

Query: 63  FQDIKPTVLIGTSGMGRTFTKEVMEAIAAINEKPIILALLNPTSQFECTAEEAYTWTEGR 122
              IKPTVLIG+SG+GR+FTKEV+EA+++INE+P+I+AL NPT+Q ECTAEEAYTW++GR
Sbjct: 426 ---IKPTVLIGSSGVGRSFTKEVIEAMSSINERPLIMALSNPTTQSECTAEEAYTWSKGR 485

Query: 123 AIFASGGPFDPVEYKRNVFVSKQANKAYIFLGFRLGLIMFGTIRIRDDMLLAACK 178
           AIFASG PFDPVEY+  VFVS QAN AYIF GF LGL++ G IR+ DDMLLAA +
Sbjct: 486 AIFASGSPFDPVEYEGKVFVSTQANNAYIFPGFGLGLVISGAIRVHDDMLLAAAE 511

[Close]

BLAST of CaUC02G032330 vs. ExPASy Swiss-Prot
Match: P12628 (NADP-dependent malic enzyme OS=Phaseolus vulgaris OX=3885 GN=ME1 PE=2 SV=1)

HSP 1 Score: 199.1 bits (505), Expect = 5.0e-50
Identity = 103/173 (59.54%), Postives = 121/173 (69.94%), Query Frame = 0


Query: 3   GLVVSSCKESLQLLKQPWAHKHEPIKDHVGVVKVFFLTSPFKICIRVKRQRFHSFYLPIS 62
           GL+VSS  ESLQ  K+PWAH+HEP+K  +  VK                           
Sbjct: 374 GLIVSSRLESLQQFKKPWAHEHEPVKGLLEAVKA-------------------------- 433

Query: 63  FQDIKPTVLIGTSGMGRTFTKEVMEAIAAINEKPIILALLNPTSQFECTAEEAYTWTEGR 122
              IKPTVLIG+SG G+TFTKEV+E +A++NEKP+ILAL NPTSQ ECTAEEAYTW++GR
Sbjct: 434 ---IKPTVLIGSSGAGKTFTKEVVETMASLNEKPLILALSNPTSQSECTAEEAYTWSKGR 493

Query: 123 AIFASGGPFDPVEYKRNVFVSKQANKAYIFLGFRLGLIMFGTIRIRDDMLLAA 176
           AIFASG PFDPVEY+  +FV  QAN AYIF GF LGLIM G IR+RD+MLLAA
Sbjct: 494 AIFASGSPFDPVEYEGKLFVPGQANNAYIFPGFGLGLIMSGAIRVRDEMLLAA 517

[Close]

BLAST of CaUC02G032330 vs. ExPASy Swiss-Prot
Match: P34105 (NADP-dependent malic enzyme OS=Populus trichocarpa OX=3694 PE=2 SV=3)

HSP 1 Score: 198.7 bits (504), Expect = 6.5e-50
Identity = 102/175 (58.29%), Postives = 122/175 (69.71%), Query Frame = 0


Query: 3   GLVVSSCKESLQLLKQPWAHKHEPIKDHVGVVKVFFLTSPFKICIRVKRQRFHSFYLPIS 62
           GL+VSS KESLQ  K+PWAH+HEP+K  + VVK                           
Sbjct: 376 GLIVSSRKESLQHFKKPWAHEHEPVKGLLEVVKA-------------------------- 435

Query: 63  FQDIKPTVLIGTSGMGRTFTKEVMEAIAAINEKPIILALLNPTSQFECTAEEAYTWTEGR 122
              IKP VLIGTSG+G+TFTKEV+EA+A+ NEKP+ILAL NPTSQ ECTA+EAYTWT+G+
Sbjct: 436 ---IKPIVLIGTSGVGKTFTKEVIEAMASFNEKPLILALSNPTSQSECTAQEAYTWTKGK 495

Query: 123 AIFASGGPFDPVEYKRNVFVSKQANKAYIFLGFRLGLIMFGTIRIRDDMLLAACK 178
           AIFASG PFDPVEY+  VFV  Q+N AYIF G  LGL++ G IR+ DDMLLAA +
Sbjct: 496 AIFASGSPFDPVEYEGKVFVPGQSNNAYIFPGLGLGLVISGAIRVHDDMLLAAAE 521

[Close]

BLAST of CaUC02G032330 vs. ExPASy Swiss-Prot
Match: Q9CA83 (NADP-dependent malic enzyme 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NADP-ME4 PE=1 SV=1)

HSP 1 Score: 198.4 bits (503), Expect = 8.5e-50
Identity = 103/173 (59.54%), Postives = 120/173 (69.36%), Query Frame = 0


Query: 3   GLVVSSCKESLQLLKQPWAHKHEPIKDHVGVVKVFFLTSPFKICIRVKRQRFHSFYLPIS 62
           GL+VSS KES+Q  K+PWAH HEPI++ V  VK                           
Sbjct: 431 GLIVSSRKESIQHFKKPWAHDHEPIRELVDAVKA-------------------------- 490

Query: 63  FQDIKPTVLIGTSGMGRTFTKEVMEAIAAINEKPIILALLNPTSQFECTAEEAYTWTEGR 122
              IKPTVLIGTSG+G+TFT++V+E +A +NEKPIIL+L NPTSQ ECTAEEAYTW++GR
Sbjct: 491 ---IKPTVLIGTSGVGQTFTQDVVETMAKLNEKPIILSLSNPTSQSECTAEEAYTWSQGR 550

Query: 123 AIFASGGPFDPVEYKRNVFVSKQANKAYIFLGFRLGLIMFGTIRIRDDMLLAA 176
           AIFASG PF PVEY+   FV  QAN AYIF GF LGLIM GTIR+ DDMLLAA
Sbjct: 551 AIFASGSPFAPVEYEGKTFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAA 574

[Close]

BLAST of CaUC02G032330 vs. ExPASy Swiss-Prot
Match: Q9LYG3 (NADP-dependent malic enzyme 2 OS=Arabidopsis thaliana OX=3702 GN=NADP-ME2 PE=1 SV=1)

HSP 1 Score: 197.6 bits (501), Expect = 1.4e-49
Identity = 102/173 (58.96%), Postives = 117/173 (67.63%), Query Frame = 0


Query: 3   GLVVSSCKESLQLLKQPWAHKHEPIKDHVGVVKVFFLTSPFKICIRVKRQRFHSFYLPIS 62
           GL+VSS KESLQ  KQPWAH+H+P+KD +G V                            
Sbjct: 373 GLIVSSRKESLQHFKQPWAHEHKPVKDLIGAVNA-------------------------- 432

Query: 63  FQDIKPTVLIGTSGMGRTFTKEVMEAIAAINEKPIILALLNPTSQFECTAEEAYTWTEGR 122
              IKPTVLIGTSG+G+TFTKEV+EA+A  NEKP+ILAL NPTSQ ECTAE+AYTWT+GR
Sbjct: 433 ---IKPTVLIGTSGVGQTFTKEVVEAMATNNEKPLILALSNPTSQAECTAEQAYTWTKGR 492

Query: 123 AIFASGGPFDPVEYKRNVFVSKQANKAYIFLGFRLGLIMFGTIRIRDDMLLAA 176
           AIF SG PFDPV Y    ++  QAN  YIF G  LGLIM G IR+RDDMLLAA
Sbjct: 493 AIFGSGSPFDPVVYDGKTYLPGQANNCYIFPGLGLGLIMSGAIRVRDDMLLAA 516

[Close]

BLAST of CaUC02G032330 vs. ExPASy TrEMBL
Match: A0A6J1GPG8 (Malic enzyme OS=Cucurbita moschata OX=3662 GN=LOC111455943 PE=3 SV=1)

HSP 1 Score: 252.3 bits (643), Expect = 1.8e-63
Identity = 129/173 (74.57%), Postives = 142/173 (82.08%), Query Frame = 0


Query: 3   GLVVSSCKESLQLLKQPWAHKHEPIKDHVGVVKVFFLTSPFKICIRVKRQRFHSFYLPIS 62
           GL+VSS KESLQ  K+PWAH+HEPI+D V  VKV FL SPFK   +  +       +PIS
Sbjct: 416 GLIVSSRKESLQHFKKPWAHEHEPIQDLVDAVKVSFLPSPFKFLHKPMK-------VPIS 475

Query: 63  FQDIKPTVLIGTSGMGRTFTKEVMEAIAAINEKPIILALLNPTSQFECTAEEAYTWTEGR 122
           FQDIKPTVLIGTSG+GRTFTK+V+EA+AAIN+KPIILAL NPTSQ ECTAEEAYTWTEGR
Sbjct: 476 FQDIKPTVLIGTSGVGRTFTKDVVEAMAAINKKPIILALSNPTSQSECTAEEAYTWTEGR 535

Query: 123 AIFASGGPFDPVEYKRNVFVSKQANKAYIFLGFRLGLIMFGTIRIRDDMLLAA 176
           AIFASG PFDPVEY  NVFV  QAN AYIF GF LGLIM GTIR+RDDMLLAA
Sbjct: 536 AIFASGSPFDPVEYNGNVFVPGQANNAYIFPGFGLGLIMSGTIRVRDDMLLAA 581

[Close]

BLAST of CaUC02G032330 vs. ExPASy TrEMBL
Match: A0A5D3BWG1 (Malic enzyme OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G00290 PE=3 SV=1)

HSP 1 Score: 224.9 bits (572), Expect = 3.1e-55
Identity = 118/173 (68.21%), Postives = 128/173 (73.99%), Query Frame = 0


Query: 3   GLVVSSCKESLQLLKQPWAHKHEPIKDHVGVVKVFFLTSPFKICIRVKRQRFHSFYLPIS 62
           GL+VSS KESLQ  K+PWAH+HEPIKD +  VK                           
Sbjct: 353 GLIVSSRKESLQHFKKPWAHEHEPIKDLIDAVK--------------------------- 412

Query: 63  FQDIKPTVLIGTSGMGRTFTKEVMEAIAAINEKPIILALLNPTSQFECTAEEAYTWTEGR 122
             DIKPTVLIGTSG+GRTFTK+V+EA+AAINEKPIILAL NPTSQ ECTAEEAYTWTEGR
Sbjct: 413 --DIKPTVLIGTSGVGRTFTKDVVEAMAAINEKPIILALSNPTSQSECTAEEAYTWTEGR 472

Query: 123 AIFASGGPFDPVEYKRNVFVSKQANKAYIFLGFRLGLIMFGTIRIRDDMLLAA 176
           AIFASG PFDPVEY+ NVFV  QAN AYIF GF LGLIM GTIR+RDDMLLAA
Sbjct: 473 AIFASGSPFDPVEYEGNVFVPGQANNAYIFPGFGLGLIMSGTIRVRDDMLLAA 496

[Close]

BLAST of CaUC02G032330 vs. ExPASy TrEMBL
Match: A0A1S3CGW8 (Malic enzyme OS=Cucumis melo OX=3656 GN=LOC103500782 PE=3 SV=1)

HSP 1 Score: 224.9 bits (572), Expect = 3.1e-55
Identity = 118/173 (68.21%), Postives = 128/173 (73.99%), Query Frame = 0


Query: 3   GLVVSSCKESLQLLKQPWAHKHEPIKDHVGVVKVFFLTSPFKICIRVKRQRFHSFYLPIS 62
           GL+VSS KESLQ  K+PWAH+HEPIKD +  VK                           
Sbjct: 353 GLIVSSRKESLQHFKKPWAHEHEPIKDLIDAVK--------------------------- 412

Query: 63  FQDIKPTVLIGTSGMGRTFTKEVMEAIAAINEKPIILALLNPTSQFECTAEEAYTWTEGR 122
             DIKPTVLIGTSG+GRTFTK+V+EA+AAINEKPIILAL NPTSQ ECTAEEAYTWTEGR
Sbjct: 413 --DIKPTVLIGTSGVGRTFTKDVVEAMAAINEKPIILALSNPTSQSECTAEEAYTWTEGR 472

Query: 123 AIFASGGPFDPVEYKRNVFVSKQANKAYIFLGFRLGLIMFGTIRIRDDMLLAA 176
           AIFASG PFDPVEY+ NVFV  QAN AYIF GF LGLIM GTIR+RDDMLLAA
Sbjct: 473 AIFASGSPFDPVEYEGNVFVPGQANNAYIFPGFGLGLIMSGTIRVRDDMLLAA 496

[Close]

BLAST of CaUC02G032330 vs. ExPASy TrEMBL
Match: A0A6J1GPG6 (Malic enzyme OS=Cucurbita moschata OX=3662 GN=LOC111455943 PE=3 SV=1)

HSP 1 Score: 221.9 bits (564), Expect = 2.6e-54
Identity = 117/173 (67.63%), Postives = 127/173 (73.41%), Query Frame = 0


Query: 3   GLVVSSCKESLQLLKQPWAHKHEPIKDHVGVVKVFFLTSPFKICIRVKRQRFHSFYLPIS 62
           GL+VSS KESLQ  K+PWAH+HEPI+D V  VK                           
Sbjct: 416 GLIVSSRKESLQHFKKPWAHEHEPIQDLVDAVK--------------------------- 475

Query: 63  FQDIKPTVLIGTSGMGRTFTKEVMEAIAAINEKPIILALLNPTSQFECTAEEAYTWTEGR 122
             DIKPTVLIGTSG+GRTFTK+V+EA+AAIN+KPIILAL NPTSQ ECTAEEAYTWTEGR
Sbjct: 476 --DIKPTVLIGTSGVGRTFTKDVVEAMAAINKKPIILALSNPTSQSECTAEEAYTWTEGR 535

Query: 123 AIFASGGPFDPVEYKRNVFVSKQANKAYIFLGFRLGLIMFGTIRIRDDMLLAA 176
           AIFASG PFDPVEY  NVFV  QAN AYIF GF LGLIM GTIR+RDDMLLAA
Sbjct: 536 AIFASGSPFDPVEYNGNVFVPGQANNAYIFPGFGLGLIMSGTIRVRDDMLLAA 559

[Close]

BLAST of CaUC02G032330 vs. ExPASy TrEMBL
Match: A0A6J1JSW4 (Malic enzyme OS=Cucurbita maxima OX=3661 GN=LOC111488596 PE=3 SV=1)

HSP 1 Score: 220.7 bits (561), Expect = 5.9e-54
Identity = 116/173 (67.05%), Postives = 127/173 (73.41%), Query Frame = 0


Query: 3   GLVVSSCKESLQLLKQPWAHKHEPIKDHVGVVKVFFLTSPFKICIRVKRQRFHSFYLPIS 62
           GL+VSS KESLQ  K+PWAH+H+PI+D V  VK                           
Sbjct: 416 GLIVSSRKESLQHFKKPWAHEHQPIQDLVDAVK--------------------------- 475

Query: 63  FQDIKPTVLIGTSGMGRTFTKEVMEAIAAINEKPIILALLNPTSQFECTAEEAYTWTEGR 122
             DIKPTVLIGTSG+GRTFTK+V+EA+AAIN+KPIILAL NPTSQ ECTAEEAYTWTEGR
Sbjct: 476 --DIKPTVLIGTSGVGRTFTKDVVEAMAAINKKPIILALSNPTSQSECTAEEAYTWTEGR 535

Query: 123 AIFASGGPFDPVEYKRNVFVSKQANKAYIFLGFRLGLIMFGTIRIRDDMLLAA 176
           AIFASG PFDPVEY  NVFV  QAN AYIF GF LGLIM GTIR+RDDMLLAA
Sbjct: 536 AIFASGSPFDPVEYNGNVFVPGQANNAYIFPGFGLGLIMSGTIRVRDDMLLAA 559

[Close]

BLAST of CaUC02G032330 vs. TAIR 10
Match: AT2G19900.1 (NADP-malic enzyme 1 )

HSP 1 Score: 204.1 bits (518), Expect = 1.1e-52
Identity = 101/175 (57.71%), Postives = 127/175 (72.57%), Query Frame = 0


Query: 3   GLVVSSCKESLQLLKQPWAHKHEPIKDHVGVVKVFFLTSPFKICIRVKRQRFHSFYLPIS 62
           GL+V+S K+SLQ  K+PWAH+HEP+KD +G +K                           
Sbjct: 366 GLIVNSRKDSLQDFKKPWAHEHEPVKDLLGAIKA-------------------------- 425

Query: 63  FQDIKPTVLIGTSGMGRTFTKEVMEAIAAINEKPIILALLNPTSQFECTAEEAYTWTEGR 122
              IKPTVLIG+SG+GR+FTKEV+EA+++INE+P+I+AL NPT+Q ECTAEEAYTW++GR
Sbjct: 426 ---IKPTVLIGSSGVGRSFTKEVIEAMSSINERPLIMALSNPTTQSECTAEEAYTWSKGR 485

Query: 123 AIFASGGPFDPVEYKRNVFVSKQANKAYIFLGFRLGLIMFGTIRIRDDMLLAACK 178
           AIFASG PFDPVEY+  VFVS QAN AYIF GF LGL++ G IR+ DDMLLAA +
Sbjct: 486 AIFASGSPFDPVEYEGKVFVSTQANNAYIFPGFGLGLVISGAIRVHDDMLLAAAE 511

[Close]

BLAST of CaUC02G032330 vs. TAIR 10
Match: AT1G79750.1 (NADP-malic enzyme 4 )

HSP 1 Score: 198.4 bits (503), Expect = 6.0e-51
Identity = 103/173 (59.54%), Postives = 120/173 (69.36%), Query Frame = 0


Query: 3   GLVVSSCKESLQLLKQPWAHKHEPIKDHVGVVKVFFLTSPFKICIRVKRQRFHSFYLPIS 62
           GL+VSS KES+Q  K+PWAH HEPI++ V  VK                           
Sbjct: 431 GLIVSSRKESIQHFKKPWAHDHEPIRELVDAVKA-------------------------- 490

Query: 63  FQDIKPTVLIGTSGMGRTFTKEVMEAIAAINEKPIILALLNPTSQFECTAEEAYTWTEGR 122
              IKPTVLIGTSG+G+TFT++V+E +A +NEKPIIL+L NPTSQ ECTAEEAYTW++GR
Sbjct: 491 ---IKPTVLIGTSGVGQTFTQDVVETMAKLNEKPIILSLSNPTSQSECTAEEAYTWSQGR 550

Query: 123 AIFASGGPFDPVEYKRNVFVSKQANKAYIFLGFRLGLIMFGTIRIRDDMLLAA 176
           AIFASG PF PVEY+   FV  QAN AYIF GF LGLIM GTIR+ DDMLLAA
Sbjct: 551 AIFASGSPFAPVEYEGKTFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAA 574

[Close]

BLAST of CaUC02G032330 vs. TAIR 10
Match: AT5G11670.1 (NADP-malic enzyme 2 )

HSP 1 Score: 197.6 bits (501), Expect = 1.0e-50
Identity = 102/173 (58.96%), Postives = 117/173 (67.63%), Query Frame = 0


Query: 3   GLVVSSCKESLQLLKQPWAHKHEPIKDHVGVVKVFFLTSPFKICIRVKRQRFHSFYLPIS 62
           GL+VSS KESLQ  KQPWAH+H+P+KD +G V                            
Sbjct: 373 GLIVSSRKESLQHFKQPWAHEHKPVKDLIGAVNA-------------------------- 432

Query: 63  FQDIKPTVLIGTSGMGRTFTKEVMEAIAAINEKPIILALLNPTSQFECTAEEAYTWTEGR 122
              IKPTVLIGTSG+G+TFTKEV+EA+A  NEKP+ILAL NPTSQ ECTAE+AYTWT+GR
Sbjct: 433 ---IKPTVLIGTSGVGQTFTKEVVEAMATNNEKPLILALSNPTSQAECTAEQAYTWTKGR 492

Query: 123 AIFASGGPFDPVEYKRNVFVSKQANKAYIFLGFRLGLIMFGTIRIRDDMLLAA 176
           AIF SG PFDPV Y    ++  QAN  YIF G  LGLIM G IR+RDDMLLAA
Sbjct: 493 AIFGSGSPFDPVVYDGKTYLPGQANNCYIFPGLGLGLIMSGAIRVRDDMLLAA 516

[Close]

BLAST of CaUC02G032330 vs. TAIR 10
Match: AT5G25880.1 (NADP-malic enzyme 3 )

HSP 1 Score: 197.2 bits (500), Expect = 1.3e-50
Identity = 102/173 (58.96%), Postives = 116/173 (67.05%), Query Frame = 0


Query: 3   GLVVSSCKESLQLLKQPWAHKHEPIKDHVGVVKVFFLTSPFKICIRVKRQRFHSFYLPIS 62
           GL+VS  KESLQ  KQPWAH H+P+K+ +  V                            
Sbjct: 373 GLIVSERKESLQHFKQPWAHDHKPVKELLAAVNA-------------------------- 432

Query: 63  FQDIKPTVLIGTSGMGRTFTKEVMEAIAAINEKPIILALLNPTSQFECTAEEAYTWTEGR 122
              IKPTVLIGTSG+G+TFTKEV+EA+A +NEKP+ILAL NPTSQ ECTAEEAYTWT+GR
Sbjct: 433 ---IKPTVLIGTSGVGKTFTKEVVEAMATLNEKPLILALSNPTSQAECTAEEAYTWTKGR 492

Query: 123 AIFASGGPFDPVEYKRNVFVSKQANKAYIFLGFRLGLIMFGTIRIRDDMLLAA 176
           AIFASG PFDPV+Y    F   QAN  YIF G  LGLIM G IR+RDDMLLAA
Sbjct: 493 AIFASGSPFDPVQYDGKKFTPGQANNCYIFPGLGLGLIMSGAIRVRDDMLLAA 516

[Close]

BLAST of CaUC02G032330 vs. TAIR 10
Match: AT2G13560.1 (NAD-dependent malic enzyme 1 )

HSP 1 Score: 88.6 bits (218), Expect = 6.7e-18
Identity = 47/117 (40.17%), Postives = 70/117 (59.83%), Query Frame = 0


Query: 64  QDIKPTVLIGTSGMGRTFTKEVMEAI-AAINEKPIILALLNPTSQFECTAEEAYTWTEGR 123
           +++KP VL+G S +G  F+KEV+EA+  + + +P I A+ NPT   ECT ++A++     
Sbjct: 424 REVKPDVLLGLSAVGGLFSKEVLEAMKGSTSTRPAIFAMSNPTKNAECTPQDAFSILGEN 483

Query: 124 AIFASGGPFDPVEY-KRNVFVSKQANKAYIFLGFRLGLIMFGTIRIRDDMLLAACKC 179
            IFASG PF  VE+   +V    Q N  Y+F G  LG ++ G   + D ML AA +C
Sbjct: 484 MIFASGSPFKNVEFGNGHVGHCNQGNNMYLFPGIGLGTLLSGAPIVSDGMLQAASEC 540

The following BLAST results are available for this feature:

BLAST of CaUC02G032330 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023547383.1	3.8e-63	74.57	NADP-dependent malic enzyme-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023...	[more]
XP_022953374.1	3.8e-63	74.57	NADP-dependent malic enzyme-like isoform X1 [Cucurbita moschata]	[more]
XP_008462428.1	6.5e-55	68.21	PREDICTED: NADP-dependent malic enzyme 4, chloroplastic-like isoform X2 [Cucumis...	[more]
XP_038900186.1	8.4e-55	68.21	NADP-dependent malic enzyme-like isoform X1 [Benincasa hispida]	[more]
XP_038900188.1	8.4e-55	68.21	NADP-dependent malic enzyme 4, chloroplastic-like isoform X2 [Benincasa hispida]	[more]

BLAST of CaUC02G032330 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O82191	1.5e-51	57.71	NADP-dependent malic enzyme 1 OS=Arabidopsis thaliana OX=3702 GN=NADP-ME1 PE=1 S...	[more]
P12628	5.0e-50	59.54	NADP-dependent malic enzyme OS=Phaseolus vulgaris OX=3885 GN=ME1 PE=2 SV=1	[more]
P34105	6.5e-50	58.29	NADP-dependent malic enzyme OS=Populus trichocarpa OX=3694 PE=2 SV=3	[more]
Q9CA83	8.5e-50	59.54	NADP-dependent malic enzyme 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=...	[more]
Q9LYG3	1.4e-49	58.96	NADP-dependent malic enzyme 2 OS=Arabidopsis thaliana OX=3702 GN=NADP-ME2 PE=1 S...	[more]

BLAST of CaUC02G032330 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GPG8	1.8e-63	74.57	Malic enzyme OS=Cucurbita moschata OX=3662 GN=LOC111455943 PE=3 SV=1	[more]
A0A5D3BWG1	3.1e-55	68.21	Malic enzyme OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G00290 P...	[more]
A0A1S3CGW8	3.1e-55	68.21	Malic enzyme OS=Cucumis melo OX=3656 GN=LOC103500782 PE=3 SV=1	[more]
A0A6J1GPG6	2.6e-54	67.63	Malic enzyme OS=Cucurbita moschata OX=3662 GN=LOC111455943 PE=3 SV=1	[more]
A0A6J1JSW4	5.9e-54	67.05	Malic enzyme OS=Cucurbita maxima OX=3661 GN=LOC111488596 PE=3 SV=1	[more]

BLAST of CaUC02G032330 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G19900.1	1.1e-52	57.71	NADP-malic enzyme 1	[more]
AT1G79750.1	6.0e-51	59.54	NADP-malic enzyme 4	[more]
AT5G11670.1	1.0e-50	58.96	NADP-malic enzyme 2	[more]
AT5G25880.1	1.3e-50	58.96	NADP-malic enzyme 3	[more]
AT2G13560.1	6.7e-18	40.17	NAD-dependent malic enzyme 1	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (USVL246-FR2) v1
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012302	Malic enzyme, NAD-binding	SMART	SM00919	Malic_M_2	coord: 5..213 e-value: 5.0E-9 score: -32.6
IPR012302	Malic enzyme, NAD-binding	PFAM	PF03949	Malic_M	coord: 3..178 e-value: 2.1E-40 score: 138.6
None	No IPR available	GENE3D	3.40.50.720		coord: 1..189 e-value: 2.4E-44 score: 153.9
None	No IPR available	PANTHER	PTHR23406	MALIC ENZYME-RELATED	coord: 63..177 coord: 3..36
None	No IPR available	PANTHER	PTHR23406:SF65	MALIC ENZYME	coord: 63..177 coord: 3..36
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 63..182

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CaUC02G032330.1	CaUC02G032330.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006108	malate metabolic process
biological_process	GO:0006090	pyruvate metabolic process
cellular_component	GO:0009507	chloroplast
cellular_component	GO:0016020	membrane
molecular_function	GO:0004471	malate dehydrogenase (decarboxylating) (NAD+) activity
molecular_function	GO:0004473	malate dehydrogenase (decarboxylating) (NADP+) activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0051287	NAD binding