CSPI07G23300 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI07G23300
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionHistidine phosphotransfer protein
LocationChr7: 19618812 .. 19620993 (-)
RNA-Seq ExpressionCSPI07G23300
SyntenyCSPI07G23300
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AGCTTAACCGCCTCTAACTATTTCTTTCCTTTTCTTTGTTTCAACCTCATAAAAGTTCTACTTTTGGAGTCATTTGCACTTCCTCAAATGGAGAAAACACAGTTGCGTAGGCAGCTTGCTAACATTAGGCAGTCCCTTTTTGATCAGGTAATTAATGATGTTCACATTTAATTCCACCACCCTAAAAGTTTACTAATTTAACAACATATCTCATAGTTAGGAAGTGTTAGTTAAGTTGTAATTCCTACAATGAATGACAATTCCCTTCGATTTCATGTGTTATATAGCCTGTATGCTTTTGTTCATATGTGAACTGGAAGAGCAGTGTAAAAAGACTATACTTGGCTTGCTATATCAGTGATATTTTCAGTTTAGCGTGTTAAGTGTAATATCAAATGCTTCGTTTTGACATCAAATTCCGGCTGGCCATTTGTCAGGGATTTCTTGACGAACAGTTTGTGCAGTTAGAGGAACTGCAAGATGATGCTAACCCAAACTTTGTGGAGGAAATTGTTACATTGTACTACAGAGACTCATCCAGACTGATTCTTAGCATAGAGCAAGCACTGTATGCTTCTTATTTTCTTTTGCTGCTTACCAAATTTTGACCTAAGAAAGTCTCTTTGTTATATAATTAATCTCCAAAATTTTCATCTCAAATGCAGACAGAAGAGCCCTCTGGACTTCAATAAGTTGGATGCCCTTATGCACCAATTTAAAGGAAGTAGCTCAAGGTAACCCAAATATTTTGAATAACTGGCAATGTTAACAAAATTTGAACATAAAAGTCTTTAAAAAAAAATTAAAAAAAATGTGAAAGAGAGAGGGTGAGGTGGCCATGGGTCGGAGTATATACTCTGATGGGCACTAGTTAAATATAGCCAAACAAATCTTCCTGTTGAAAAAACACTCATGTTCTTTTTGTAATGGGTGCAGTATTGGAGCCAAAAAGGTGAAAGCTGAGTGCACACAATTGAGGGAATATTGCAAGGCAGGAAGTGGAGAAGGGTAAATATCTATCATACATATCCTTTTTCTTATTCCCTTCAACGTGGACGGCTGAGATTGATCGAACATGAATTTGATCCCTTTCCCTGCTCCTCCATTTTAAGTATACATCTATATTTTCATTTTTTTAAAAATATATATCAGTTTTTAAATTAACAAGTATAGTAGAAAGTAACATGAGCTTTGAGATGTATGGTTCAGTCTTTGCATGCATGCAATGGAAACAATGACAGTAGGAAGGTAGACAATAACATATTATTATTATTATTATCGTTTACATCACATGCTCACTTAGCTACATGAAGAAAAAGCAACCAACTTTTTCAAACTTGTTCAGTTAGGTCATTACTGTGCATCACTGTTGATGGTATCTTATACTAATTAAAGAGATTTTCCTTAAAGCTTTCCATTAAAAATCTGCTTATTTACTCTAAAAAGTAAGTTTTGTTTTCTAAATCTTCTTAAAAAGTGATTTTGACAAATTTTCCATTCCTAACTTTTAATTAAAAGGGGTTATTTAAAAACAAATGTTATTCTAGAAGGTATATATATGCATGTAATCTACTTAATTGTTGTTTGGGTTCAAAGTCTCGACTCCCTAGTGCTACTTGAGAAACAGTTTCCTTTCCCACTTCCAAACACTTTTTAAGGCTTTAAAGAGTTTGTACAGGATCACCTATTTTATTATCATTTGTTAACTGTTTACTAAAAACAAATATCTCCTTTTTTTATAATGAAAACAGATGCATGAGAACATTCCAACAACTGAAGAAAGAATATACAAGTCTGAGAAAGAAGCTTGAAGCCTATTTTCAGGTTTGTATAAAAAAGCTTAGCTCCCATTCTTTGGTTTCCTTCGTTACTTTTTCATTCCAATATAATATAATCTATTTTCTGCAAGCTAAAGGTAATTAACATTTAAGTTTTGTGGTCTAATCACAGCTCGCAAGACAGGCTGGGCCCATGGAATCTGCATGTCGGCCCAAGTAATCATATGTGGAAGACAAATGGAGGACAGAGCTGATGTGGGAAGATGTGATTGGAGATCTTTATGTTTCTCTAAATTATGTAAAGCTCCAATTGTGGATTGGAGCAGCCTGTGGGGCTTATAGGTTGCTTTTCTTTTCTTTTCTTTTCTTTTCTTTTTCTTTTCTTCTTTTTGGGTTCATGAATG

mRNA sequence

AGCTTAACCGCCTCTAACTATTTCTTTCCTTTTCTTTGTTTCAACCTCATAAAAGTTCTACTTTTGGAGTCATTTGCACTTCCTCAAATGGAGAAAACACAGTTGCGTAGGCAGCTTGCTAACATTAGGCAGTCCCTTTTTGATCAGGGATTTCTTGACGAACAGTTTGTGCAGTTAGAGGAACTGCAAGATGATGCTAACCCAAACTTTGTGGAGGAAATTGTTACATTGTACTACAGAGACTCATCCAGACTGATTCTTAGCATAGAGCAAGCACTACAGAAGAGCCCTCTGGACTTCAATAAGTTGGATGCCCTTATGCACCAATTTAAAGGAAGTAGCTCAAGTATTGGAGCCAAAAAGGTGAAAGCTGAGTGCACACAATTGAGGGAATATTGCAAGGCAGGAAGTGGAGAAGGATGCATGAGAACATTCCAACAACTGAAGAAAGAATATACAAGTCTGAGAAAGAAGCTTGAAGCCTATTTTCAGCTCGCAAGACAGGCTGGGCCCATGGAATCTGCATGTCGGCCCAAGTAATCATATGTGGAAGACAAATGGAGGACAGAGCTGATGTGGGAAGATGTGATTGGAGATCTTTATGTTTCTCTAAATTATGTAAAGCTCCAATTGTGGATTGGAGCAGCCTGTGGGGCTTATAGGTTGCTTTTCTTTTCTTTTCTTTTCTTTTCTTTTTCTTTTCTTCTTTTTGGGTTCATGAATG

Coding sequence (CDS)

ATGGAGAAAACACAGTTGCGTAGGCAGCTTGCTAACATTAGGCAGTCCCTTTTTGATCAGGGATTTCTTGACGAACAGTTTGTGCAGTTAGAGGAACTGCAAGATGATGCTAACCCAAACTTTGTGGAGGAAATTGTTACATTGTACTACAGAGACTCATCCAGACTGATTCTTAGCATAGAGCAAGCACTACAGAAGAGCCCTCTGGACTTCAATAAGTTGGATGCCCTTATGCACCAATTTAAAGGAAGTAGCTCAAGTATTGGAGCCAAAAAGGTGAAAGCTGAGTGCACACAATTGAGGGAATATTGCAAGGCAGGAAGTGGAGAAGGATGCATGAGAACATTCCAACAACTGAAGAAAGAATATACAAGTCTGAGAAAGAAGCTTGAAGCCTATTTTCAGCTCGCAAGACAGGCTGGGCCCATGGAATCTGCATGTCGGCCCAAGTAA

Protein sequence

MEKTQLRRQLANIRQSLFDQGFLDEQFVQLEELQDDANPNFVEEIVTLYYRDSSRLILSIEQALQKSPLDFNKLDALMHQFKGSSSSIGAKKVKAECTQLREYCKAGSGEGCMRTFQQLKKEYTSLRKKLEAYFQLARQAGPMESACRPK*
Homology
BLAST of CSPI07G23300 vs. ExPASy Swiss-Prot
Match: Q9LU15 (Histidine-containing phosphotransfer protein 4 OS=Arabidopsis thaliana OX=3702 GN=AHP4 PE=1 SV=2)

HSP 1 Score: 179.9 bits (455), Expect = 2.2e-44
Identity = 84/119 (70.59%), Postives = 106/119 (89.08%), Query Frame = 0

Query: 20  QGFLDEQFVQLEELQDDANPNFVEEIVTLYYRDSSRLILSIEQALQKSPLDFNKLDALMH 79
           QG+LDEQF++LEELQDDANPNFVEE+  LY++DS+RLI +I+QAL++   DFN+LD+ MH
Sbjct: 9   QGYLDEQFMELEELQDDANPNFVEEVSALYFKDSARLINNIDQALERGSFDFNRLDSYMH 68

Query: 80  QFKGSSSSIGAKKVKAECTQLREYCKAGSGEGCMRTFQQLKKEYTSLRKKLEAYFQLAR 139
           QFKGSS+SIGA KVKAECT  REYC+AG+ EGC+RTFQQLKKE+++LRKKLE YFQ ++
Sbjct: 69  QFKGSSTSIGASKVKAECTTFREYCRAGNAEGCLRTFQQLKKEHSTLRKKLEHYFQASQ 127

BLAST of CSPI07G23300 vs. ExPASy Swiss-Prot
Match: Q6F303 (Pseudo histidine-containing phosphotransfer protein 5 OS=Oryza sativa subsp. japonica OX=39947 GN=PHP5 PE=2 SV=1)

HSP 1 Score: 178.3 bits (451), Expect = 6.4e-44
Identity = 85/149 (57.05%), Postives = 116/149 (77.85%), Query Frame = 0

Query: 1   MEKTQLRRQLANIRQSLFDQGFLDEQFVQLEELQDDANPNFVEEIVTLYYRDSSRLILSI 60
           ME   LRRQ A++++SLFDQG+LDEQF Q+E+LQD+ANPNF EE+V+L+++DS+R++L+ 
Sbjct: 1   MEYGNLRRQAASLKKSLFDQGYLDEQFCQVEDLQDEANPNFAEEVVSLFFKDSTRVMLNF 60

Query: 61  EQALQKSPLDFNKLDALMHQFKGSSSSIGAKKVKAECTQLREYCKAGSGEGCMRTFQQLK 120
           EQA++K P DF + D  M Q KGS SSIGA +VK ECT  R +C   + EGC R+FQ++K
Sbjct: 61  EQAIEKHPKDFARWDTHMQQLKGSCSSIGASRVKNECTSFRNFCGEENAEGCTRSFQKVK 120

Query: 121 KEYTSLRKKLEAYFQLARQAGPMESACRP 150
           +E+  LR+K E+YFQL RQAGP  +A RP
Sbjct: 121 REHAVLRQKWESYFQLLRQAGPAGTATRP 149

BLAST of CSPI07G23300 vs. ExPASy Swiss-Prot
Match: Q0DK78 (Pseudo histidine-containing phosphotransfer protein 2 OS=Oryza sativa subsp. japonica OX=39947 GN=PHP2 PE=2 SV=3)

HSP 1 Score: 164.5 bits (415), Expect = 9.5e-40
Identity = 79/146 (54.11%), Postives = 114/146 (78.08%), Query Frame = 0

Query: 1   MEKTQLRRQLANIRQSLFDQGFLDEQFVQLEELQDDANPNFVEEIVTLYYRDSSRLILSI 60
           ME + LRRQ+  ++++LFDQG+LDEQF QLEELQD+++PNFVEE+  L+++DSSRL+ +I
Sbjct: 1   MEYSNLRRQIIFMKKNLFDQGYLDEQFNQLEELQDESSPNFVEEVAALFFKDSSRLLTNI 60

Query: 61  EQALQKSPLDFNKLDALMHQFKGSSSSIGAKKVKAECTQLREYCKAGSGEGCMRTFQQLK 120
           EQA+ K P DF +LD+L+ Q KGS SSIGA ++K EC+  +  C   + EGC R+ Q++K
Sbjct: 61  EQAIDKYPQDFYRLDSLVQQLKGSGSSIGALRMKNECSVFKVNCNDRNLEGCRRSLQKMK 120

Query: 121 KEYTSLRKKLEAYFQLARQAGPMESA 147
           +E+ +L++KLE+YFQL RQ GP + A
Sbjct: 121 REHATLKQKLESYFQLLRQVGPRDYA 146

BLAST of CSPI07G23300 vs. ExPASy Swiss-Prot
Match: Q0JJE3 (Pseudo histidine-containing phosphotransfer protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=PHP1 PE=3 SV=2)

HSP 1 Score: 164.1 bits (414), Expect = 1.2e-39
Identity = 78/130 (60.00%), Postives = 104/130 (80.00%), Query Frame = 0

Query: 20  QGFLDEQFVQLEELQDDANPNFVEEIVTLYYRDSSRLILSIEQALQKSPLDFNKLDALMH 79
           +G+LDEQF Q+E+LQD+A+PNFVEE+VTL+++DS RL+ +IEQAL+K P DFN+ D  M 
Sbjct: 56  KGYLDEQFCQVEDLQDEASPNFVEEVVTLFFKDSGRLMSNIEQALEKYPRDFNRWDTYMQ 115

Query: 80  QFKGSSSSIGAKKVKAECTQLREYCKAGSGEGCMRTFQQLKKEYTSLRKKLEAYFQLARQ 139
           Q KGS SSIGA ++K EC   R+ C  G+ EGCMR+FQ++K+E+  LR+KLE+YFQL RQ
Sbjct: 116 QLKGSCSSIGASRMKNECMSFRDSCGQGNVEGCMRSFQKVKREHAVLRQKLESYFQLLRQ 175

Query: 140 AGPMESACRP 150
           AGP  +A RP
Sbjct: 176 AGPAGAATRP 185

BLAST of CSPI07G23300 vs. ExPASy Swiss-Prot
Match: Q9ZNV9 (Histidine-containing phosphotransfer protein 1 OS=Arabidopsis thaliana OX=3702 GN=AHP1 PE=1 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 2.9e-28
Identity = 60/139 (43.17%), Postives = 97/139 (69.78%), Query Frame = 0

Query: 1   MEKTQLRRQLANIRQSLFDQGFLDEQFVQLEELQDDANPNFVEEIVTLYYRDSSRLILSI 60
           M+  Q ++ L +  +SLF +G LD QF+QL++LQD++NP+FV ++VTL+++DS R++  +
Sbjct: 1   MDLVQKQKSLQDYTKSLFLEGILDSQFLQLQQLQDESNPDFVSQVVTLFFQDSDRILNDL 60

Query: 61  EQALQKSPLDFNKLDALMHQFKGSSSSIGAKKVKAECTQLREYCKAGSGEGCMRTFQQLK 120
             +L +  +DF K+D  +HQ KGSSSSIGA++VK  C   R +C+  + E C R  QQ+K
Sbjct: 61  SLSLDQQVVDFKKVDPHVHQLKGSSSSIGAQRVKNACVVFRSFCEQQNVEACHRCLQQVK 120

Query: 121 KEYTSLRKKLEAYFQLARQ 140
           +EY  ++ +LE  F+L +Q
Sbjct: 121 QEYYLVKNRLETLFKLEQQ 139

BLAST of CSPI07G23300 vs. ExPASy TrEMBL
Match: A0A0A0KB29 (HPt domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G452370 PE=4 SV=1)

HSP 1 Score: 294.3 bits (752), Expect = 2.9e-76
Identity = 150/150 (100.00%), Postives = 150/150 (100.00%), Query Frame = 0

Query: 1   MEKTQLRRQLANIRQSLFDQGFLDEQFVQLEELQDDANPNFVEEIVTLYYRDSSRLILSI 60
           MEKTQLRRQLANIRQSLFDQGFLDEQFVQLEELQDDANPNFVEEIVTLYYRDSSRLILSI
Sbjct: 1   MEKTQLRRQLANIRQSLFDQGFLDEQFVQLEELQDDANPNFVEEIVTLYYRDSSRLILSI 60

Query: 61  EQALQKSPLDFNKLDALMHQFKGSSSSIGAKKVKAECTQLREYCKAGSGEGCMRTFQQLK 120
           EQALQKSPLDFNKLDALMHQFKGSSSSIGAKKVKAECTQLREYCKAGSGEGCMRTFQQLK
Sbjct: 61  EQALQKSPLDFNKLDALMHQFKGSSSSIGAKKVKAECTQLREYCKAGSGEGCMRTFQQLK 120

Query: 121 KEYTSLRKKLEAYFQLARQAGPMESACRPK 151
           KEYTSLRKKLEAYFQLARQAGPMESACRPK
Sbjct: 121 KEYTSLRKKLEAYFQLARQAGPMESACRPK 150

BLAST of CSPI07G23300 vs. ExPASy TrEMBL
Match: A0A5A7UBP6 (Histidine-containing phosphotransfer protein 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G00320 PE=4 SV=1)

HSP 1 Score: 291.6 bits (745), Expect = 1.9e-75
Identity = 148/150 (98.67%), Postives = 150/150 (100.00%), Query Frame = 0

Query: 1   MEKTQLRRQLANIRQSLFDQGFLDEQFVQLEELQDDANPNFVEEIVTLYYRDSSRLILSI 60
           MEKTQLRRQLANIRQSLFDQGFLDEQFVQLEELQDDANPNFVEEIVTLYYRDSSRLILSI
Sbjct: 1   MEKTQLRRQLANIRQSLFDQGFLDEQFVQLEELQDDANPNFVEEIVTLYYRDSSRLILSI 60

Query: 61  EQALQKSPLDFNKLDALMHQFKGSSSSIGAKKVKAECTQLREYCKAGSGEGCMRTFQQLK 120
           EQALQKSPLDFNKLDALMHQFKGSSSSIGAKKVKAECTQLREYCKAGSGEGCMRTFQQLK
Sbjct: 61  EQALQKSPLDFNKLDALMHQFKGSSSSIGAKKVKAECTQLREYCKAGSGEGCMRTFQQLK 120

Query: 121 KEYTSLRKKLEAYFQLARQAGPMESACRPK 151
           KEYT+LRKKLEAYFQLARQAGP+ESACRPK
Sbjct: 121 KEYTTLRKKLEAYFQLARQAGPIESACRPK 150

BLAST of CSPI07G23300 vs. ExPASy TrEMBL
Match: A0A1S3CGY5 (histidine-containing phosphotransfer protein 4 OS=Cucumis melo OX=3656 GN=LOC103500781 PE=4 SV=1)

HSP 1 Score: 291.6 bits (745), Expect = 1.9e-75
Identity = 148/150 (98.67%), Postives = 150/150 (100.00%), Query Frame = 0

Query: 1   MEKTQLRRQLANIRQSLFDQGFLDEQFVQLEELQDDANPNFVEEIVTLYYRDSSRLILSI 60
           MEKTQLRRQLANIRQSLFDQGFLDEQFVQLEELQDDANPNFVEEIVTLYYRDSSRLILSI
Sbjct: 1   MEKTQLRRQLANIRQSLFDQGFLDEQFVQLEELQDDANPNFVEEIVTLYYRDSSRLILSI 60

Query: 61  EQALQKSPLDFNKLDALMHQFKGSSSSIGAKKVKAECTQLREYCKAGSGEGCMRTFQQLK 120
           EQALQKSPLDFNKLDALMHQFKGSSSSIGAKKVKAECTQLREYCKAGSGEGCMRTFQQLK
Sbjct: 61  EQALQKSPLDFNKLDALMHQFKGSSSSIGAKKVKAECTQLREYCKAGSGEGCMRTFQQLK 120

Query: 121 KEYTSLRKKLEAYFQLARQAGPMESACRPK 151
           KEYT+LRKKLEAYFQLARQAGP+ESACRPK
Sbjct: 121 KEYTTLRKKLEAYFQLARQAGPIESACRPK 150

BLAST of CSPI07G23300 vs. ExPASy TrEMBL
Match: A0A6J1JPT6 (histidine-containing phosphotransfer protein 4 OS=Cucurbita maxima OX=3661 GN=LOC111488738 PE=4 SV=1)

HSP 1 Score: 287.0 bits (733), Expect = 4.7e-74
Identity = 144/150 (96.00%), Postives = 150/150 (100.00%), Query Frame = 0

Query: 1   MEKTQLRRQLANIRQSLFDQGFLDEQFVQLEELQDDANPNFVEEIVTLYYRDSSRLILSI 60
           MEKTQLRRQLANIRQSLFDQGFLDEQFVQLEELQDDANPNFVEEIVTLYYRDSSRLI+SI
Sbjct: 1   MEKTQLRRQLANIRQSLFDQGFLDEQFVQLEELQDDANPNFVEEIVTLYYRDSSRLIVSI 60

Query: 61  EQALQKSPLDFNKLDALMHQFKGSSSSIGAKKVKAECTQLREYCKAGSGEGCMRTFQQLK 120
           EQALQKSPLDFNKLDALMHQFKGSSSSIGAKKVKAECTQLREYC+AGSGEGC+RTFQQLK
Sbjct: 61  EQALQKSPLDFNKLDALMHQFKGSSSSIGAKKVKAECTQLREYCRAGSGEGCLRTFQQLK 120

Query: 121 KEYTSLRKKLEAYFQLARQAGPMESACRPK 151
           KEYT+LRKKLEAYFQLARQAGP++SACRPK
Sbjct: 121 KEYTTLRKKLEAYFQLARQAGPIDSACRPK 150

BLAST of CSPI07G23300 vs. ExPASy TrEMBL
Match: A0A6J1GPH3 (histidine-containing phosphotransfer protein 4-like OS=Cucurbita moschata OX=3662 GN=LOC111455947 PE=4 SV=1)

HSP 1 Score: 285.8 bits (730), Expect = 1.0e-73
Identity = 143/150 (95.33%), Postives = 150/150 (100.00%), Query Frame = 0

Query: 1   MEKTQLRRQLANIRQSLFDQGFLDEQFVQLEELQDDANPNFVEEIVTLYYRDSSRLILSI 60
           MEKTQLRRQLANIRQSLFDQGFLDEQFVQLEELQDDANPNFVEEIVTLYYRDSSRLI+SI
Sbjct: 1   MEKTQLRRQLANIRQSLFDQGFLDEQFVQLEELQDDANPNFVEEIVTLYYRDSSRLIVSI 60

Query: 61  EQALQKSPLDFNKLDALMHQFKGSSSSIGAKKVKAECTQLREYCKAGSGEGCMRTFQQLK 120
           EQALQKSPLDFNKLDALMHQFKGSSSSIGAKKVKAECTQLREYC+AGSG+GC+RTFQQLK
Sbjct: 61  EQALQKSPLDFNKLDALMHQFKGSSSSIGAKKVKAECTQLREYCRAGSGDGCLRTFQQLK 120

Query: 121 KEYTSLRKKLEAYFQLARQAGPMESACRPK 151
           KEYT+LRKKLEAYFQLARQAGP++SACRPK
Sbjct: 121 KEYTTLRKKLEAYFQLARQAGPIDSACRPK 150

BLAST of CSPI07G23300 vs. NCBI nr
Match: XP_004141666.1 (histidine-containing phosphotransfer protein 4 [Cucumis sativus] >XP_031745195.1 histidine-containing phosphotransfer protein 4 [Cucumis sativus] >KGN45582.1 hypothetical protein Csa_015843 [Cucumis sativus])

HSP 1 Score: 294.3 bits (752), Expect = 6.1e-76
Identity = 150/150 (100.00%), Postives = 150/150 (100.00%), Query Frame = 0

Query: 1   MEKTQLRRQLANIRQSLFDQGFLDEQFVQLEELQDDANPNFVEEIVTLYYRDSSRLILSI 60
           MEKTQLRRQLANIRQSLFDQGFLDEQFVQLEELQDDANPNFVEEIVTLYYRDSSRLILSI
Sbjct: 1   MEKTQLRRQLANIRQSLFDQGFLDEQFVQLEELQDDANPNFVEEIVTLYYRDSSRLILSI 60

Query: 61  EQALQKSPLDFNKLDALMHQFKGSSSSIGAKKVKAECTQLREYCKAGSGEGCMRTFQQLK 120
           EQALQKSPLDFNKLDALMHQFKGSSSSIGAKKVKAECTQLREYCKAGSGEGCMRTFQQLK
Sbjct: 61  EQALQKSPLDFNKLDALMHQFKGSSSSIGAKKVKAECTQLREYCKAGSGEGCMRTFQQLK 120

Query: 121 KEYTSLRKKLEAYFQLARQAGPMESACRPK 151
           KEYTSLRKKLEAYFQLARQAGPMESACRPK
Sbjct: 121 KEYTSLRKKLEAYFQLARQAGPMESACRPK 150

BLAST of CSPI07G23300 vs. NCBI nr
Match: XP_008462426.1 (PREDICTED: histidine-containing phosphotransfer protein 4 [Cucumis melo])

HSP 1 Score: 291.6 bits (745), Expect = 3.9e-75
Identity = 148/150 (98.67%), Postives = 150/150 (100.00%), Query Frame = 0

Query: 1   MEKTQLRRQLANIRQSLFDQGFLDEQFVQLEELQDDANPNFVEEIVTLYYRDSSRLILSI 60
           MEKTQLRRQLANIRQSLFDQGFLDEQFVQLEELQDDANPNFVEEIVTLYYRDSSRLILSI
Sbjct: 1   MEKTQLRRQLANIRQSLFDQGFLDEQFVQLEELQDDANPNFVEEIVTLYYRDSSRLILSI 60

Query: 61  EQALQKSPLDFNKLDALMHQFKGSSSSIGAKKVKAECTQLREYCKAGSGEGCMRTFQQLK 120
           EQALQKSPLDFNKLDALMHQFKGSSSSIGAKKVKAECTQLREYCKAGSGEGCMRTFQQLK
Sbjct: 61  EQALQKSPLDFNKLDALMHQFKGSSSSIGAKKVKAECTQLREYCKAGSGEGCMRTFQQLK 120

Query: 121 KEYTSLRKKLEAYFQLARQAGPMESACRPK 151
           KEYT+LRKKLEAYFQLARQAGP+ESACRPK
Sbjct: 121 KEYTTLRKKLEAYFQLARQAGPIESACRPK 150

BLAST of CSPI07G23300 vs. NCBI nr
Match: KAA0051045.1 (histidine-containing phosphotransfer protein 4 [Cucumis melo var. makuwa] >TYK03840.1 histidine-containing phosphotransfer protein 4 [Cucumis melo var. makuwa])

HSP 1 Score: 291.6 bits (745), Expect = 3.9e-75
Identity = 148/150 (98.67%), Postives = 150/150 (100.00%), Query Frame = 0

Query: 1   MEKTQLRRQLANIRQSLFDQGFLDEQFVQLEELQDDANPNFVEEIVTLYYRDSSRLILSI 60
           MEKTQLRRQLANIRQSLFDQGFLDEQFVQLEELQDDANPNFVEEIVTLYYRDSSRLILSI
Sbjct: 1   MEKTQLRRQLANIRQSLFDQGFLDEQFVQLEELQDDANPNFVEEIVTLYYRDSSRLILSI 60

Query: 61  EQALQKSPLDFNKLDALMHQFKGSSSSIGAKKVKAECTQLREYCKAGSGEGCMRTFQQLK 120
           EQALQKSPLDFNKLDALMHQFKGSSSSIGAKKVKAECTQLREYCKAGSGEGCMRTFQQLK
Sbjct: 61  EQALQKSPLDFNKLDALMHQFKGSSSSIGAKKVKAECTQLREYCKAGSGEGCMRTFQQLK 120

Query: 121 KEYTSLRKKLEAYFQLARQAGPMESACRPK 151
           KEYT+LRKKLEAYFQLARQAGP+ESACRPK
Sbjct: 121 KEYTTLRKKLEAYFQLARQAGPIESACRPK 150

BLAST of CSPI07G23300 vs. NCBI nr
Match: XP_038899247.1 (histidine-containing phosphotransfer protein 4-like [Benincasa hispida])

HSP 1 Score: 289.3 bits (739), Expect = 2.0e-74
Identity = 146/150 (97.33%), Postives = 150/150 (100.00%), Query Frame = 0

Query: 1   MEKTQLRRQLANIRQSLFDQGFLDEQFVQLEELQDDANPNFVEEIVTLYYRDSSRLILSI 60
           MEKTQLRRQLANIRQSLFDQG+LDEQFVQLEELQDDANPNFVEEIVTLYYRDSSRLILSI
Sbjct: 1   MEKTQLRRQLANIRQSLFDQGYLDEQFVQLEELQDDANPNFVEEIVTLYYRDSSRLILSI 60

Query: 61  EQALQKSPLDFNKLDALMHQFKGSSSSIGAKKVKAECTQLREYCKAGSGEGCMRTFQQLK 120
           EQALQKSPLDFNKLDALMHQFKGSSSSIGAKKVKAECTQLREYCKAGSGEGC+RTFQQLK
Sbjct: 61  EQALQKSPLDFNKLDALMHQFKGSSSSIGAKKVKAECTQLREYCKAGSGEGCLRTFQQLK 120

Query: 121 KEYTSLRKKLEAYFQLARQAGPMESACRPK 151
           KEYT+LRKKLEAYFQLARQAGP+ESACRPK
Sbjct: 121 KEYTTLRKKLEAYFQLARQAGPIESACRPK 150

BLAST of CSPI07G23300 vs. NCBI nr
Match: XP_022992432.1 (histidine-containing phosphotransfer protein 4 [Cucurbita maxima])

HSP 1 Score: 287.0 bits (733), Expect = 9.7e-74
Identity = 144/150 (96.00%), Postives = 150/150 (100.00%), Query Frame = 0

Query: 1   MEKTQLRRQLANIRQSLFDQGFLDEQFVQLEELQDDANPNFVEEIVTLYYRDSSRLILSI 60
           MEKTQLRRQLANIRQSLFDQGFLDEQFVQLEELQDDANPNFVEEIVTLYYRDSSRLI+SI
Sbjct: 1   MEKTQLRRQLANIRQSLFDQGFLDEQFVQLEELQDDANPNFVEEIVTLYYRDSSRLIVSI 60

Query: 61  EQALQKSPLDFNKLDALMHQFKGSSSSIGAKKVKAECTQLREYCKAGSGEGCMRTFQQLK 120
           EQALQKSPLDFNKLDALMHQFKGSSSSIGAKKVKAECTQLREYC+AGSGEGC+RTFQQLK
Sbjct: 61  EQALQKSPLDFNKLDALMHQFKGSSSSIGAKKVKAECTQLREYCRAGSGEGCLRTFQQLK 120

Query: 121 KEYTSLRKKLEAYFQLARQAGPMESACRPK 151
           KEYT+LRKKLEAYFQLARQAGP++SACRPK
Sbjct: 121 KEYTTLRKKLEAYFQLARQAGPIDSACRPK 150

BLAST of CSPI07G23300 vs. TAIR 10
Match: AT3G16360.2 (HPT phosphotransmitter 4 )

HSP 1 Score: 219.2 bits (557), Expect = 2.3e-57
Identity = 105/145 (72.41%), Postives = 130/145 (89.66%), Query Frame = 0

Query: 6   LRRQLANIRQSLFDQGFLDEQFVQLEELQDDANPNFVEEIVTLYYRDSSRLILSIEQALQ 65
           ++RQ+A I+QSLFDQG+LDEQF++LEELQDDANPNFVEE+  LY++DS+RLI +I+QAL+
Sbjct: 1   MQRQVALIKQSLFDQGYLDEQFMELEELQDDANPNFVEEVSALYFKDSARLINNIDQALE 60

Query: 66  KSPLDFNKLDALMHQFKGSSSSIGAKKVKAECTQLREYCKAGSGEGCMRTFQQLKKEYTS 125
           +   DFN+LD+ MHQFKGSS+SIGA KVKAECT  REYC+AG+ EGC+RTFQQLKKE+++
Sbjct: 61  RGSFDFNRLDSYMHQFKGSSTSIGASKVKAECTTFREYCRAGNAEGCLRTFQQLKKEHST 120

Query: 126 LRKKLEAYFQLARQAGPMESACRPK 151
           LRKKLE YFQLARQAGP E+A RPK
Sbjct: 121 LRKKLEHYFQLARQAGPKETARRPK 145

BLAST of CSPI07G23300 vs. TAIR 10
Match: AT3G16360.1 (HPT phosphotransmitter 4 )

HSP 1 Score: 179.9 bits (455), Expect = 1.6e-45
Identity = 84/119 (70.59%), Postives = 106/119 (89.08%), Query Frame = 0

Query: 20  QGFLDEQFVQLEELQDDANPNFVEEIVTLYYRDSSRLILSIEQALQKSPLDFNKLDALMH 79
           QG+LDEQF++LEELQDDANPNFVEE+  LY++DS+RLI +I+QAL++   DFN+LD+ MH
Sbjct: 9   QGYLDEQFMELEELQDDANPNFVEEVSALYFKDSARLINNIDQALERGSFDFNRLDSYMH 68

Query: 80  QFKGSSSSIGAKKVKAECTQLREYCKAGSGEGCMRTFQQLKKEYTSLRKKLEAYFQLAR 139
           QFKGSS+SIGA KVKAECT  REYC+AG+ EGC+RTFQQLKKE+++LRKKLE YFQ ++
Sbjct: 69  QFKGSSTSIGASKVKAECTTFREYCRAGNAEGCLRTFQQLKKEHSTLRKKLEHYFQASQ 127

BLAST of CSPI07G23300 vs. TAIR 10
Match: AT3G21510.1 (histidine-containing phosphotransmitter 1 )

HSP 1 Score: 126.3 bits (316), Expect = 2.0e-29
Identity = 60/139 (43.17%), Postives = 97/139 (69.78%), Query Frame = 0

Query: 1   MEKTQLRRQLANIRQSLFDQGFLDEQFVQLEELQDDANPNFVEEIVTLYYRDSSRLILSI 60
           M+  Q ++ L +  +SLF +G LD QF+QL++LQD++NP+FV ++VTL+++DS R++  +
Sbjct: 1   MDLVQKQKSLQDYTKSLFLEGILDSQFLQLQQLQDESNPDFVSQVVTLFFQDSDRILNDL 60

Query: 61  EQALQKSPLDFNKLDALMHQFKGSSSSIGAKKVKAECTQLREYCKAGSGEGCMRTFQQLK 120
             +L +  +DF K+D  +HQ KGSSSSIGA++VK  C   R +C+  + E C R  QQ+K
Sbjct: 61  SLSLDQQVVDFKKVDPHVHQLKGSSSSIGAQRVKNACVVFRSFCEQQNVEACHRCLQQVK 120

Query: 121 KEYTSLRKKLEAYFQLARQ 140
           +EY  ++ +LE  F+L +Q
Sbjct: 121 QEYYLVKNRLETLFKLEQQ 139

BLAST of CSPI07G23300 vs. TAIR 10
Match: AT3G29350.1 (histidine-containing phosphotransmitter 2 )

HSP 1 Score: 124.0 bits (310), Expect = 1.0e-28
Identity = 62/136 (45.59%), Postives = 97/136 (71.32%), Query Frame = 0

Query: 5   QLRRQLANIRQSLFDQGFLDEQFVQLEELQDDANPNFVEEIVTLYYRDSSRLILSIEQAL 64
           QL+RQ  +   SL+ QGFLD+QF +L++LQDD +P+FV E+++L++ D  +LI ++ +AL
Sbjct: 7   QLQRQFRDYTISLYQQGFLDDQFTELKKLQDDGSPDFVSEVLSLFFEDCVKLISNMARAL 66

Query: 65  QKS-PLDFNKLDALMHQFKGSSSSIGAKKVKAECTQLREYCKAGSGEGCMRTFQQLKKEY 124
             +  +DF+++ A +HQ KGSSSS+GAK+VK  C   +E C+A + EGC+R  QQ+  EY
Sbjct: 67  DTTGTVDFSQVGASVHQLKGSSSSVGAKRVKTLCVSFKECCEAKNYEGCVRCLQQVDIEY 126

Query: 125 TSLRKKLEAYFQLARQ 140
            +L+ KL+  F L +Q
Sbjct: 127 KALKTKLQDMFNLEKQ 142

BLAST of CSPI07G23300 vs. TAIR 10
Match: AT5G39340.1 (histidine-containing phosphotransmitter 3 )

HSP 1 Score: 121.7 bits (304), Expect = 5.0e-28
Identity = 62/136 (45.59%), Postives = 95/136 (69.85%), Query Frame = 0

Query: 5   QLRRQLANIRQSLFDQGFLDEQFVQLEELQDDANPNFVEEIVTLYYRDSSRLILSIEQAL 64
           QL+R+  +   SL+ QGFLD+QF +L++LQD+ +P+FV E+VTL++ D  +LI ++ +AL
Sbjct: 7   QLQRRFCDFTISLYHQGFLDDQFTELKKLQDECSPDFVAEVVTLFFEDCEKLISNMARAL 66

Query: 65  -QKSPLDFNKLDALMHQFKGSSSSIGAKKVKAECTQLREYCKAGSGEGCMRTFQQLKKEY 124
            Q   +DF  + + +HQ KGSSSS+GAK+VK  C  L+E C + + EGC+R  QQ+  EY
Sbjct: 67  DQTGNVDFKLVGSSVHQLKGSSSSVGAKRVKGLCVTLKECCDSQNYEGCVRCLQQVDIEY 126

Query: 125 TSLRKKLEAYFQLARQ 140
            +L+ KL+  F L +Q
Sbjct: 127 KTLKAKLQDLFNLEQQ 142

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LU152.2e-4470.59Histidine-containing phosphotransfer protein 4 OS=Arabidopsis thaliana OX=3702 G... [more]
Q6F3036.4e-4457.05Pseudo histidine-containing phosphotransfer protein 5 OS=Oryza sativa subsp. jap... [more]
Q0DK789.5e-4054.11Pseudo histidine-containing phosphotransfer protein 2 OS=Oryza sativa subsp. jap... [more]
Q0JJE31.2e-3960.00Pseudo histidine-containing phosphotransfer protein 1 OS=Oryza sativa subsp. jap... [more]
Q9ZNV92.9e-2843.17Histidine-containing phosphotransfer protein 1 OS=Arabidopsis thaliana OX=3702 G... [more]
Match NameE-valueIdentityDescription
A0A0A0KB292.9e-76100.00HPt domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G452370 PE=4 SV... [more]
A0A5A7UBP61.9e-7598.67Histidine-containing phosphotransfer protein 4 OS=Cucumis melo var. makuwa OX=11... [more]
A0A1S3CGY51.9e-7598.67histidine-containing phosphotransfer protein 4 OS=Cucumis melo OX=3656 GN=LOC103... [more]
A0A6J1JPT64.7e-7496.00histidine-containing phosphotransfer protein 4 OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A6J1GPH31.0e-7395.33histidine-containing phosphotransfer protein 4-like OS=Cucurbita moschata OX=366... [more]
Match NameE-valueIdentityDescription
XP_004141666.16.1e-76100.00histidine-containing phosphotransfer protein 4 [Cucumis sativus] >XP_031745195.1... [more]
XP_008462426.13.9e-7598.67PREDICTED: histidine-containing phosphotransfer protein 4 [Cucumis melo][more]
KAA0051045.13.9e-7598.67histidine-containing phosphotransfer protein 4 [Cucumis melo var. makuwa] >TYK03... [more]
XP_038899247.12.0e-7497.33histidine-containing phosphotransfer protein 4-like [Benincasa hispida][more]
XP_022992432.19.7e-7496.00histidine-containing phosphotransfer protein 4 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT3G16360.22.3e-5772.41HPT phosphotransmitter 4 [more]
AT3G16360.11.6e-4570.59HPT phosphotransmitter 4 [more]
AT3G21510.12.0e-2943.17histidine-containing phosphotransmitter 1 [more]
AT3G29350.11.0e-2845.59histidine-containing phosphotransmitter 2 [more]
AT5G39340.15.0e-2845.59histidine-containing phosphotransmitter 3 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 116..136
NoneNo IPR availablePANTHERPTHR28242FAMILY NOT NAMEDcoord: 4..146
NoneNo IPR availablePANTHERPTHR28242:SF43HISTIDINE-CONTAINING PHOSPHOTRANSFER PROTEIN 4coord: 4..146
IPR036641HPT domain superfamilyGENE3D1.20.120.160HPT domaincoord: 2..146
e-value: 1.8E-54
score: 185.5
IPR036641HPT domain superfamilySUPERFAMILY47226Histidine-containing phosphotransfer domain, HPT domaincoord: 5..139
IPR008207Signal transduction histidine kinase, phosphotransfer (Hpt) domainPFAMPF01627Hptcoord: 44..127
e-value: 4.0E-11
score: 43.0
IPR008207Signal transduction histidine kinase, phosphotransfer (Hpt) domainPROSITEPS50894HPTcoord: 38..133
score: 13.534058
IPR008207Signal transduction histidine kinase, phosphotransfer (Hpt) domainCDDcd00088HPTcoord: 42..107
e-value: 4.93988E-7
score: 43.5242

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI07G23300.1CSPI07G23300.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009736 cytokinin-activated signaling pathway
biological_process GO:0000160 phosphorelay signal transduction system
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0005829 cytosol
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005634 nucleus
molecular_function GO:0009927 histidine phosphotransfer kinase activity
molecular_function GO:0043424 protein histidine kinase binding