CSPI07G22800 (gene) Cucumber (PI 183967) v1

BLAST of CSPI07G22800 vs. ExPASy Swiss-Prot
Match: Q43866 (Beta-fructofuranosidase, insoluble isoenzyme CWINV1 OS=Arabidopsis thaliana OX=3702 GN=CWINV1 PE=1 SV=1)

HSP 1 Score: 769.6 bits (1986), Expect = 2.5e-221
Identity = 375/588 (63.78%), Postives = 449/588 (76.36%), Query Frame = 0

Query: 5   SSSLCFLNNIFLLIFLLFGHEFLQLQALHHVYRASHRTSHFVLHQHQQQPYRTSYHFQPP 64
           +  +C    ++LL+ LL G+  + L+A HHVY+   ++++        QPYRT +HFQPP
Sbjct: 2   TKEVCSNIGLWLLLTLLIGNYVVNLEASHHVYKRLTQSTN-TKSPSVNQPYRTGFHFQPP 61

Query: 65  KNWI---NGPMIYKGIYHFFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIFPSQPSD 124
           KNW+   NGPMIYKGIYH FYQ+NPKGAVWGNIVWAHSTSTDLINW+PH  AIFPS P D
Sbjct: 62  KNWMNDPNGPMIYKGIYHLFYQWNPKGAVWGNIVWAHSTSTDLINWDPHPPAIFPSAPFD 121

Query: 125 INGCWSGSATILPGEKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPT 184
           INGCWSGSATILP  KP ILYTGI+PKNQQVQN+A PKNLSDPYLREW KSP NPLMAP 
Sbjct: 122 INGCWSGSATILPNGKPVILYTGIDPKNQQVQNIAEPKNLSDPYLREWKKSPLNPLMAPD 181

Query: 185 PQNHINVSSFRDPTTAWLGRDGEWRVIIGSKIDARGLAIMYRSKDFVRWNQVDHPLRYKD 244
             N IN SSFRDPTTAWLG+D +WRVIIGSKI  RGLAI Y SKDF++W +   PL Y D
Sbjct: 182 AVNGINASSFRDPTTAWLGQDKKWRVIIGSKIHRRGLAITYTSKDFLKWEKSPEPLHYDD 241

Query: 245 DMGMWECPDFFPVAKTGRTGVEMKVSGSH---VKHILKVSLDDTKHDVYTVGTYNWKKDI 304
             GMWECPDFFPV + G  GVE    G     +KH+LK+SLDDTKHD YT+GTY+  KD 
Sbjct: 242 GSGMWECPDFFPVTRFGSNGVETSSFGEPNEILKHVLKISLDDTKHDYYTIGTYDRVKDK 301

Query: 305 YIPNKGSIEGYNGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQA 364
           ++P+ G        RYDYGKYYASKTFFDS K RR+LWGW NESSSV+DD++KGWSG+Q 
Sbjct: 302 FVPDNGFKMDGTAPRYDYGKYYASKTFFDSAKNRRILWGWTNESSSVEDDVEKGWSGIQT 361

Query: 365 IPRAIWLDASGKQLIQWPIVEIQKLRNNKVK-LTNEVLKKGSTIEVKGVTAAQADVEISF 424
           IPR IWLD SGKQLIQWP+ E+++LR  +VK L N+VLK GS +EV GVTAAQADVE+ F
Sbjct: 362 IPRKIWLDRSGKQLIQWPVREVERLRTKQVKNLRNKVLKSGSRLEVYGVTAAQADVEVLF 421

Query: 425 KVENLKKAEILKPEWKDPQLLCSQKGTSSTTEGGSVGPFGLLVLASNDLKEYTSVFFTVF 484
           KV +L+KA++++P W DPQL+CS+   S  +    +GPFGL+VLAS +L+EYTSV+F +F
Sbjct: 422 KVRDLEKADVIEPSWTDPQLICSKMNVSVKS---GLGPFGLMVLASKNLEEYTSVYFRIF 481

Query: 485 K---KQHKYVVLMCGDQSRSSLHSNNDKTTYGAFLDVDPTRENLSLRSLIDHSIVESFGI 544
           K     +KYVVLMC DQSRSSL  +NDKTTYGAF+D++P  + LSLR+LIDHS+VESFG 
Sbjct: 482 KARQNSNKYVVLMCSDQSRSSLKEDNDKTTYGAFVDINP-HQPLSLRALIDHSVVESFGG 541

Query: 545 KGKGCITARVYPTLAIGDDVSLYAFNNGTDSVTITKLTAWSMKKAHIN 583
           KG+ CIT+RVYP LAIG    L+AFN G  SV +  L AWSM  A I+
Sbjct: 542 KGRACITSRVYPKLAIGKSSHLFAFNYGYQSVDVLNLNAWSMNSAQIS 584

BLAST of CSPI07G22800 vs. ExPASy Swiss-Prot
Match: Q67XZ3 (Beta-fructofuranosidase, insoluble isoenzyme CWINV3 OS=Arabidopsis thaliana OX=3702 GN=CWINV3 PE=1 SV=2)

HSP 1 Score: 716.5 bits (1848), Expect = 2.5e-205
Identity = 344/547 (62.89%), Postives = 415/547 (75.87%), Query Frame = 0

Query: 48  HQHQQQPYRTSYHFQPPKNWI---NGPMIYKGIYHFFYQYNPKGAVWG-NIVWAHSTSTD 107
           HQ   QPYRT YHFQP KNW+   NGPMIYKGIYH FYQYNP GAVW   IVW HSTS D
Sbjct: 31  HQDLNQPYRTGYHFQPLKNWMNDPNGPMIYKGIYHLFYQYNPYGAVWDVRIVWGHSTSVD 90

Query: 108 LINWEPHDHAIFPSQPSDINGCWSGSATILPGEKPAILYTGINPKNQQVQNLAVPKNLSD 167
           L+NW     A  PSQPSDINGCWSGS TILP  KP ILYTGI+    QVQN+AVP N+SD
Sbjct: 91  LVNWISQPPAFNPSQPSDINGCWSGSVTILPNGKPVILYTGIDQNKGQVQNVAVPVNISD 150

Query: 168 PYLREWVKSPKNPLMAPTPQNHINVSSFRDPTTAWLGRDGEWRVIIGSKI-DARGLAIMY 227
           PYLREW K P+NPLM     N IN   FRDPTTAWLGRDGEWRVI+GS   D RGLAI+Y
Sbjct: 151 PYLREWSKPPQNPLMTTNAVNGINPDRFRDPTTAWLGRDGEWRVIVGSSTDDRRGLAILY 210

Query: 228 RSKDFVRWNQVDHPLRYKDDMGMWECPDFFPVAKTGRTGVEMKVSGSH-VKHILKVSLDD 287
           +S+DF  W Q   PL Y+D  GMWECPDFFPV+ TG  GVE    G + +KH+LKVSL +
Sbjct: 211 KSRDFFNWTQSMKPLHYEDLTGMWECPDFFPVSITGSDGVETSSVGENGIKHVLKVSLIE 270

Query: 288 TKHDVYTVGTYNWKKDIYIPNKGSIEGYNGLRYDYGKYYASKTFFDSQKKRRVLWGWVNE 347
           T HD YT+G+Y+ +KD+Y+P+ G ++  +  R DYGKYYASKTF+D  KKRR+LWGWVNE
Sbjct: 271 TLHDYYTIGSYDREKDVYVPDLGFVQNESAPRLDYGKYYASKTFYDDVKKRRILWGWVNE 330

Query: 348 SSSVDDDIKKGWSGVQAIPRAIWLDASGKQLIQWPIVEIQKLRNNKVKLTNEVLKKGSTI 407
           SS   DDI+KGWSG+Q+ PR IWLD SGK+L+QWPI EI+ LR  +V    +VLK GST+
Sbjct: 331 SSPAKDDIEKGWSGLQSFPRKIWLDESGKELLQWPIEEIETLRGQQVNWQKKVLKAGSTL 390

Query: 408 EVKGVTAAQADVEISFKVENLKKAEILKPEWKDPQLLCSQKGTSSTTEGGSVGPFGLLVL 467
           +V GVTAAQADVE+SFKV+ L+KA++++P W DPQ +CSQ G  S   G  +GPFGL+VL
Sbjct: 391 QVHGVTAAQADVEVSFKVKELEKADVIEPSWTDPQKICSQ-GDLSVMSG--LGPFGLMVL 450

Query: 468 ASNDLKEYTSVFFTVFK------KQHKYVVLMCGDQSRSSLHSNNDKTTYGAFLDVDPTR 527
           ASND++EYTSV+F +FK      K+ KYVVLMC DQSRSSL+  NDK+T+GAF+ +DP+ 
Sbjct: 451 ASNDMEEYTSVYFRIFKSNDDTNKKTKYVVLMCSDQSRSSLNDENDKSTFGAFVAIDPSH 510

Query: 528 ENLSLRSLIDHSIVESFGIKGKGCITARVYPTLAIGDDVSLYAFNNGTDSVTITKLTAWS 583
           + +SLR+LIDHSIVES+G  G+ CIT+RVYP LAIG++ +L+ FN GT SV I  L+AWS
Sbjct: 511 QTISLRTLIDHSIVESYGGGGRTCITSRVYPKLAIGENANLFVFNKGTQSVDILTLSAWS 570

BLAST of CSPI07G22800 vs. ExPASy Swiss-Prot
Match: Q43089 (Beta-fructofuranosidase, cell wall isozyme OS=Pisum sativum OX=3888 GN=BFRUCT1 PE=2 SV=1)

HSP 1 Score: 711.1 bits (1834), Expect = 1.1e-203
Identity = 349/556 (62.77%), Postives = 413/556 (74.28%), Query Frame = 0

Query: 7   SLCFLNNIFLLIFLLFGHEFLQLQALHHVYRASHRTSHFVLHQHQQQPYRTSYHFQPPKN 66
           S  FL ++F LI++      + ++A HHVY+     S     +   QPYRT+YHFQP KN
Sbjct: 4   SSIFLLSLFSLIYV------IPIEATHHVYQTLETLSSHHSSKSNHQPYRTAYHFQPLKN 63

Query: 67  WI---NGPMIYKGIYHFFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIFPSQPSDIN 126
           WI   NGPM Y G YH FYQYNPKGAVWGNIVWAHS S DL+NW P DHAI PSQPSDI 
Sbjct: 64  WINDPNGPMRYGGFYHLFYQYNPKGAVWGNIVWAHSVSKDLVNWTPLDHAIHPSQPSDIK 123

Query: 127 GCWSGSATILPGEKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQ 186
           GCWSGSATILPG KPAILYTGI+P N QVQN+A+PKN+SDP LREW KSPKNPLM PT  
Sbjct: 124 GCWSGSATILPGGKPAILYTGIDPNNHQVQNIAIPKNMSDPLLREWKKSPKNPLMEPTIA 183

Query: 187 NHINVSSFRDPTTAWLGRDGEWRVIIGSKIDARGLAIMYRSKDFVRWNQVDHPLRYKDDM 246
           N IN SSFRDPTT+WLG+DG WRV+IGSKID +G+AI+Y+SK+FV W +  HPL   +  
Sbjct: 184 NKINSSSFRDPTTSWLGKDGFWRVLIGSKIDTKGMAILYKSKNFVDWVEAKHPLHSAEGT 243

Query: 247 GMWECPDFFPVAKTG--RTGVEMKVSG-SHVKHILKVSLDDTKHDVYTVGTYNWKKDIYI 306
           GMWECPDF+PV      RTGV+   +G   V+H+LKVSLDDTKHD Y +G+Y+  KD+++
Sbjct: 244 GMWECPDFYPVLDKNLLRTGVDTSRNGDDDVRHVLKVSLDDTKHDHYLIGSYDVVKDVFV 303

Query: 307 PNKGSIEGYNGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIP 366
           P  G  +    LRYDYGKYYASKTFFD  K RR+L GWVNESSSV DD+KKGWSG+  IP
Sbjct: 304 PENGFEDNGFVLRYDYGKYYASKTFFDDGKNRRILLGWVNESSSVADDVKKGWSGIHTIP 363

Query: 367 RAIWLDASGKQLIQWPIVEIQKLRNNKVKLTNEVLKKGSTIEVKGVTAAQADVEISFKVE 426
           R IWL  SGKQL+QWP+ EI+ LR N V    +V+K G  I + GV + QADVEISF++ 
Sbjct: 364 REIWLHESGKQLVQWPVKEIENLRMNPVNWPTKVIKGGERISITGVDSVQADVEISFEIS 423

Query: 427 NLKKAEILKPEWKDPQLLCSQKGTSSTTEGGSVGPFGLLVLASNDLKEYTSVFFTVFKKQ 486
           +L K E L+ +W DPQLLCSQKG       G VGPFGLLV AS  LKEYT+VFF +FK Q
Sbjct: 424 DLGKVESLR-KWIDPQLLCSQKGAGVK---GGVGPFGLLVFASQGLKEYTAVFFRIFKYQ 483

Query: 487 HKYVVLMCGDQSRSSLHSNNDKTTYGAFLDVDPTRENLSLRSLIDHSIVESFGIKGKGCI 546
            K +VLMC DQSRSSL+ +ND T+YG F+DVDP  E LSLR+LIDHS+VESFG +G+ C+
Sbjct: 484 DKNLVLMCSDQSRSSLNKDNDMTSYGTFVDVDPLHEKLSLRTLIDHSVVESFGGEGRACV 543

Query: 547 TARVYPTLAIGDDVSL 557
           TARVYPTLAI D   L
Sbjct: 544 TARVYPTLAIHDKAML 549

BLAST of CSPI07G22800 vs. ExPASy Swiss-Prot
Match: Q70XE6 (Fructan 6-exohydrolase OS=Beta vulgaris OX=161934 GN=6-FEH PE=1 SV=1)

HSP 1 Score: 704.9 bits (1818), Expect = 7.6e-202
Identity = 345/565 (61.06%), Postives = 415/565 (73.45%), Query Frame = 0

Query: 30  QALHHVYRASHRTSH--FVLHQHQ----QQPYRTSYHFQPPKNWI---NGPMIYKGIYHF 89
           QA+HH     H   H   +++ HQ      PYRT+YHFQ PKNW+   NGPMIYKGIYH 
Sbjct: 29  QAIHH-----HDDDHDDMLINDHQMINDDDPYRTAYHFQSPKNWMNDPNGPMIYKGIYHL 88

Query: 90  FYQYNPKGAVW-GNIVWAHSTSTDLINWEPHDHAIFPSQPSDINGCWSGSATILPGEKPA 149
           FYQY P   VW   IVW HSTSTDLINW     A+ PS+P DINGCWSGS TILP  KP 
Sbjct: 89  FYQYYPYDPVWHTEIVWGHSTSTDLINWTQQPIALSPSEPYDINGCWSGSITILPQNKPV 148

Query: 150 ILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMA--PTPQNHINVSSFRDPTTA 209
           ILYTGIN KN QVQNLA+PKNLSDPYL+EW+K P+NPLMA  PT  N+IN SSFRDP+TA
Sbjct: 149 ILYTGINNKNYQVQNLALPKNLSDPYLKEWIKLPQNPLMAGTPTNNNNINASSFRDPSTA 208

Query: 210 WLGRDGEWRVIIGSKIDARGLAIMYRSKDFVRWNQVDHPLRYKDDMGMWECPDFFPVAKT 269
           W   DG+WRVI+G++   RGLA+++ S DFV+WN   +PL   +  G+WECPDFFPV   
Sbjct: 209 WQLSDGKWRVIVGTQQGKRGLAVLFTSDDFVKWNNTGNPLHSTEGNGIWECPDFFPVYVG 268

Query: 270 GRTGVEMKVSGSHVKHILKVSLDDTKHDVYTVGTYNWKKDIYIPNKGSIEGYNGLRYDYG 329
              G +  + G  VKH+LK+SL DT+++ YT+G Y+ +KDIY+P++GSIE   GLRYDYG
Sbjct: 269 KSLGADTSIIGDDVKHVLKLSLFDTQYEYYTIGRYDIEKDIYVPDEGSIESDLGLRYDYG 328

Query: 330 KYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRAIWLDASGKQLIQWPI 389
           K+YASK+FFD +  RR+LWGWVNESS   DDIKKGWSGVQAIPR + LD SGKQL+QWP+
Sbjct: 329 KFYASKSFFDDETNRRILWGWVNESSIQADDIKKGWSGVQAIPRTVVLDKSGKQLVQWPL 388

Query: 390 VEIQKLRNNKVKLTNEVLKKGSTIEVKGVTAAQADVEISFKVENLKKAEILKPEWKDPQL 449
            E+  LR N V+L ++V+K GS +E+  +TA+QADVEISFK+      E L     +PQ+
Sbjct: 389 AEVDMLRENDVELPSQVIKGGSLVEISQITASQADVEISFKIPESNYVEELDSTCTNPQI 448

Query: 450 LCSQKGTSSTTEGGSVGPFGLLVLASNDLKEYTSVFFTVFKKQHKYVVLMCGDQSRSSLH 509
           LCSQKG S     G  GPFGLL LAS  L+EYT+VFF +FK  +KYVVLMC DQ+RSSL+
Sbjct: 449 LCSQKGASIK---GRFGPFGLLTLASMGLEEYTAVFFRIFKGPNKYVVLMCSDQTRSSLN 508

Query: 510 SNNDKTTYGAFLDVDPTRENLSLRSLIDHSIVESFGIKGKGCITARVYPTLAIGDDVSLY 569
              DK ++G F+DVDP  E+LSLR LIDHSIVESF  KGK CITARVYPT+AI D   LY
Sbjct: 509 PTTDKLSFGIFVDVDPINEDLSLRILIDHSIVESFSAKGKSCITARVYPTMAINDKAKLY 568

Query: 570 AFNNGTDSVTITKLTAWSMKKAHIN 583
            FNNGT+ V ITKL+AWSMKKA IN
Sbjct: 569 VFNNGTEDVKITKLSAWSMKKAQIN 585

BLAST of CSPI07G22800 vs. ExPASy Swiss-Prot
Match: Q9LIB9 (Beta-fructofuranosidase, insoluble isoenzyme CWINV5 OS=Arabidopsis thaliana OX=3702 GN=CWINV5 PE=2 SV=2)

HSP 1 Score: 684.9 bits (1766), Expect = 8.1e-196
Identity = 329/540 (60.93%), Postives = 406/540 (75.19%), Query Frame = 0

Query: 51  QQQPYRTSYHFQPPKNWI---NGPMIYKGIYHFFYQYNPKGAVW--GNIVWAHSTSTDLI 110
           + QPYRT YHFQPPKNW+   NGPMIYKGIYH FYQ+N  GAV      VW H+TSTDLI
Sbjct: 38  KNQPYRTGYHFQPPKNWMNDPNGPMIYKGIYHLFYQWNQNGAVMDVNKTVWGHATSTDLI 97

Query: 111 NWEPHDHAIFPSQPSDINGCWSGSATILPGEKPAILYTGINPKNQQVQNLAVPKNLSDPY 170
           NW     AI PS+PSDINGCWSGS TILP  KP ILYTG +  N+QVQNL  PKNL+DPY
Sbjct: 98  NWITLSPAIRPSRPSDINGCWSGSVTILPNGKPVILYTGNDRYNRQVQNLVKPKNLTDPY 157

Query: 171 LREWVKSPKNPLMAPTPQNHINVSSFRDPTTAWLGRDGEWRVIIGSKIDARGLAIMYRSK 230
           LR W KSP+NPL+ P+P NHIN S+FRDPTTAW GRDG WR+  GS+   RGLAI++ SK
Sbjct: 158 LRHWTKSPENPLVTPSPVNHINSSAFRDPTTAWFGRDGRWRITTGSQEGRRGLAILHTSK 217

Query: 231 DFVRWNQVDHPLRYKDDMGMWECPDFFPVAKTGRTGVEMKV-SGSHVKHILKVSLDDTKH 290
           DFV W Q   PL Y D  GMWECPDFFPVA+T   G++    SG  VKH+LKVSL DT +
Sbjct: 218 DFVIWKQSPKPLHYHDGTGMWECPDFFPVARTDSRGLDTSFSSGPMVKHVLKVSLTDTFN 277

Query: 291 DVYTVGTYNWKKDIYIPNKGSIEGYNGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSS 350
           D YT+GTY+  +D+Y+P+KG ++     RYDYGK+YASKTF+DS  +RR+LWGWVNESS 
Sbjct: 278 DYYTIGTYDEVRDVYVPDKGFVQDETAPRYDYGKFYASKTFYDSVNQRRILWGWVNESSP 337

Query: 351 VDDDIKKGWSGVQAIPRAIWLDASGKQLIQWPIVEIQKLRNNKVKLTNEVLKKGSTIEVK 410
             D+IKKGW+G+QAIPR +WLD SGK+L+QWP+ EI++LR  +VK  N++LK GS +EV 
Sbjct: 338 EKDNIKKGWAGLQAIPRKVWLDESGKRLVQWPVKEIERLRTTQVKWGNKLLKGGSVMEVH 397

Query: 411 GVTAAQADVEISFKVE--NLKKAEILKPEWKDPQLLCSQKGTSSTTEGGSVGPFGLLVLA 470
           GVTA QADVE+ FKV   +L+KA++++P W DPQL+CSQ+  SS      +GPFGL+VLA
Sbjct: 398 GVTAPQADVEVFFKVSGFDLEKADVIEPGWTDPQLICSQRNASS-----GLGPFGLMVLA 457

Query: 471 SNDLKEYTSVFFTVFK---KQHKYVVLMCGDQSRSSLHSNNDKTTYGAFLDVDPTRENLS 530
           S +++EYTSV   +F+      ++VV+MC DQS SSL   NDKTTYGAFLD+ P  + +S
Sbjct: 458 SKNMEEYTSVNIRIFRAGENSKEHVVVMCSDQSTSSLEKGNDKTTYGAFLDISP-YQPIS 517

Query: 531 LRSLIDHSIVESFGIKGKGCITARVYPTLAIGDDVSLYAFNNGTDSVTITKLTAWSMKKA 580
           LR+LID SIVESFG KGK CIT+RVYP LAIG+   L+AFN G+ +V +  L+AWSMK +
Sbjct: 518 LRTLIDKSIVESFGGKGKTCITSRVYPKLAIGERTHLFAFNKGSQNVNVLSLSAWSMKSS 571

BLAST of CSPI07G22800 vs. ExPASy TrEMBL
Match: A0A2I4JRK2 (Cell wall invertase 2 OS=Cucumis sativus OX=3659 PE=2 SV=1)

HSP 1 Score: 1199.1 bits (3101), Expect = 0.0e+00
Identity = 578/585 (98.80%), Postives = 580/585 (99.15%), Query Frame = 0

Query: 1   MASYSSSLCFLNNIFLLIFLLFGHEFLQLQALHHVYRASHRTSHFVLHQHQQQPYRTSYH 60
           MASYSSSLCFLNNIFLLIFLLFGHEFLQLQALHHVYRASHRTSHFVLHQHQQQPYRTSYH
Sbjct: 1   MASYSSSLCFLNNIFLLIFLLFGHEFLQLQALHHVYRASHRTSHFVLHQHQQQPYRTSYH 60

Query: 61  FQPPKNWI---NGPMIYKGIYHFFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIFPS 120
           FQPPKNWI   NGPMIYKGIYHFFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIFPS
Sbjct: 61  FQPPKNWINDPNGPMIYKGIYHFFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIFPS 120

Query: 121 QPSDINGCWSGSATILPGEKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPL 180
           QPSDINGCWSGSATILPGEKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPL
Sbjct: 121 QPSDINGCWSGSATILPGEKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPL 180

Query: 181 MAPTPQNHINVSSFRDPTTAWLGRDGEWRVIIGSKIDARGLAIMYRSKDFVRWNQVDHPL 240
           MAPTPQNHINVSSFRDPTTAWLGRDGEWRVIIGSKIDARGLAIMYRSKDFVRWNQVDHPL
Sbjct: 181 MAPTPQNHINVSSFRDPTTAWLGRDGEWRVIIGSKIDARGLAIMYRSKDFVRWNQVDHPL 240

Query: 241 RYKDDMGMWECPDFFPVAKTGRTGVEMKVSGSHVKHILKVSLDDTKHDVYTVGTYNWKKD 300
           RYKDDMGMWECPDFFPVAKTGRTGVEMKVSGSHVKHILKVSLDDTKHDVYTVGTYNWKKD
Sbjct: 241 RYKDDMGMWECPDFFPVAKTGRTGVEMKVSGSHVKHILKVSLDDTKHDVYTVGTYNWKKD 300

Query: 301 IYIPNKGSIEGYNGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQ 360
           IYIPNKGSIEGYNGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQ
Sbjct: 301 IYIPNKGSIEGYNGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQ 360

Query: 361 AIPRAIWLDASGKQLIQWPIVEIQKLRNNKVKLTNEVLKKGSTIEVKGVTAAQADVEISF 420
           AIPRAIWLDASGKQLIQWPIVEIQKLRNNKVKLTNEVLKKGSTIEVKGVTAAQADVEISF
Sbjct: 361 AIPRAIWLDASGKQLIQWPIVEIQKLRNNKVKLTNEVLKKGSTIEVKGVTAAQADVEISF 420

Query: 421 KVENLKKAEILKPEWKDPQLLCSQKGTSSTTEGGSVGPFGLLVLASNDLKEYTSVFFTVF 480
           KVENLKKAEILKPEWKDPQLLCSQKGTSSTTEGGSVGPFGLLVLASNDLKEYTSVFFTVF
Sbjct: 421 KVENLKKAEILKPEWKDPQLLCSQKGTSSTTEGGSVGPFGLLVLASNDLKEYTSVFFTVF 480

Query: 481 KKQHKYVVLMCGDQSRSSLHSNNDKTTYGAFLDVDPTRENLSLRSLIDHSIVESFGIKGK 540
           KK HKYVVLMC DQSRSSLHSNNDKTTYGAFLDVDPTREN+SLRSLIDHSIVESFGIKGK
Sbjct: 481 KKPHKYVVLMCSDQSRSSLHSNNDKTTYGAFLDVDPTRENISLRSLIDHSIVESFGIKGK 540

Query: 541 GCITARVYPTLAIGDDVSLYAFNNGTDSVTITKLTAWSMKKAHIN 583
           GCITARVYPTLAIGD+VSLYAFNNGTDSVTITKLTAWSMKKAHIN
Sbjct: 541 GCITARVYPTLAIGDNVSLYAFNNGTDSVTITKLTAWSMKKAHIN 585

BLAST of CSPI07G22800 vs. ExPASy TrEMBL
Match: A0A0A0KCP2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G450780 PE=3 SV=1)

HSP 1 Score: 1062.0 bits (2745), Expect = 9.0e-307
Identity = 507/511 (99.22%), Postives = 509/511 (99.61%), Query Frame = 0

Query: 72  MIYKGIYHFFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIFPSQPSDINGCWSGSAT 131
           MIYKGIYHFFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIFPSQPSDINGCWSGSAT
Sbjct: 1   MIYKGIYHFFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIFPSQPSDINGCWSGSAT 60

Query: 132 ILPGEKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNHINVSSF 191
           ILPGEKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNHINVSSF
Sbjct: 61  ILPGEKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNHINVSSF 120

Query: 192 RDPTTAWLGRDGEWRVIIGSKIDARGLAIMYRSKDFVRWNQVDHPLRYKDDMGMWECPDF 251
           RDPTTAWLGRDGEWRVIIGSKIDARGLAIMYRSKDFVRWNQVDHPLRYKDDMGMWECPDF
Sbjct: 121 RDPTTAWLGRDGEWRVIIGSKIDARGLAIMYRSKDFVRWNQVDHPLRYKDDMGMWECPDF 180

Query: 252 FPVAKTGRTGVEMKVSGSHVKHILKVSLDDTKHDVYTVGTYNWKKDIYIPNKGSIEGYNG 311
           FPVAKTGRTGVEMKVSGSHVKHILKVSLDDTKHDVYTVGTYNWKKDIYIPNKGSIEGYNG
Sbjct: 181 FPVAKTGRTGVEMKVSGSHVKHILKVSLDDTKHDVYTVGTYNWKKDIYIPNKGSIEGYNG 240

Query: 312 LRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRAIWLDASGKQ 371
           LRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRAIWLDASGKQ
Sbjct: 241 LRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRAIWLDASGKQ 300

Query: 372 LIQWPIVEIQKLRNNKVKLTNEVLKKGSTIEVKGVTAAQADVEISFKVENLKKAEILKPE 431
           LIQWPIVEIQKLRNNKVKLTNEVLKKGSTIEVKGVTAAQADVEISFKVENLKKAEILKPE
Sbjct: 301 LIQWPIVEIQKLRNNKVKLTNEVLKKGSTIEVKGVTAAQADVEISFKVENLKKAEILKPE 360

Query: 432 WKDPQLLCSQKGTSSTTEGGSVGPFGLLVLASNDLKEYTSVFFTVFKKQHKYVVLMCGDQ 491
           WKDPQLLCSQKGTSSTTEGGSVGPFGLLVLASNDLKEYTSVFFTVFKK HKYVVLMC DQ
Sbjct: 361 WKDPQLLCSQKGTSSTTEGGSVGPFGLLVLASNDLKEYTSVFFTVFKKPHKYVVLMCSDQ 420

Query: 492 SRSSLHSNNDKTTYGAFLDVDPTRENLSLRSLIDHSIVESFGIKGKGCITARVYPTLAIG 551
           SRSSLHSNNDKTTYGAFLDVDPTREN+SLRSLIDHSIVESFGIKGKGCITARVYPTLAIG
Sbjct: 421 SRSSLHSNNDKTTYGAFLDVDPTRENISLRSLIDHSIVESFGIKGKGCITARVYPTLAIG 480

Query: 552 DDVSLYAFNNGTDSVTITKLTAWSMKKAHIN 583
           D+VSLYAFNNGTDSVTITKLTAWSMKKAHIN
Sbjct: 481 DNVSLYAFNNGTDSVTITKLTAWSMKKAHIN 511

BLAST of CSPI07G22800 vs. ExPASy TrEMBL
Match: A0A5A7V0R5 (Beta-fructofuranosidase, insoluble isoenzyme CWINV1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G00960 PE=3 SV=1)

HSP 1 Score: 998.0 bits (2579), Expect = 1.6e-287
Identity = 473/511 (92.56%), Postives = 489/511 (95.69%), Query Frame = 0

Query: 72  MIYKGIYHFFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIFPSQPSDINGCWSGSAT 131
           MIYKGIYH FYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAI+PSQPSDINGCWSGSAT
Sbjct: 1   MIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIYPSQPSDINGCWSGSAT 60

Query: 132 ILPGEKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNHINVSSF 191
           ILPGEKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNHIN SSF
Sbjct: 61  ILPGEKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNHINASSF 120

Query: 192 RDPTTAWLGRDGEWRVIIGSKIDARGLAIMYRSKDFVRWNQVDHPLRYKDDMGMWECPDF 251
           RDPTTAW+G DGEWRVI+GSK+DARGLA+MYRSKDFV+WNQVDHPL YKD MGMWECPDF
Sbjct: 121 RDPTTAWIGPDGEWRVIVGSKLDARGLAVMYRSKDFVKWNQVDHPLHYKDGMGMWECPDF 180

Query: 252 FPVAKTGRTGVEMKVSGSHVKHILKVSLDDTKHDVYTVGTYNWKKDIYIPNKGSIEGYNG 311
           FPVAKTGR GVE +V GS VKH+LKVSLDDTKHDVYTVGTYNW+KDIY+PNKGSIE YNG
Sbjct: 181 FPVAKTGRKGVETRVYGSQVKHVLKVSLDDTKHDVYTVGTYNWQKDIYVPNKGSIESYNG 240

Query: 312 LRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRAIWLDASGKQ 371
           LRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRA+WLDASGKQ
Sbjct: 241 LRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRAVWLDASGKQ 300

Query: 372 LIQWPIVEIQKLRNNKVKLTNEVLKKGSTIEVKGVTAAQADVEISFKVENLKKAEILKPE 431
           LIQWP+VEIQKLRNNKVKLTNEVLKKGS +EVKGVTAAQADVEISFKVEN KKAEILKPE
Sbjct: 301 LIQWPVVEIQKLRNNKVKLTNEVLKKGSIVEVKGVTAAQADVEISFKVENFKKAEILKPE 360

Query: 432 WKDPQLLCSQKGTSSTTEGGSVGPFGLLVLASNDLKEYTSVFFTVFKKQHKYVVLMCGDQ 491
           WKDPQLLCSQKGT STT+ GSVGPFGLLVLASNDLKEYTSV F VFK QHKYVVLMC DQ
Sbjct: 361 WKDPQLLCSQKGT-STTKSGSVGPFGLLVLASNDLKEYTSVSFRVFKGQHKYVVLMCSDQ 420

Query: 492 SRSSLHSNNDKTTYGAFLDVDPTRENLSLRSLIDHSIVESFGIKGKGCITARVYPTLAIG 551
           SRSSL+SN+DKTTYG FLDVDPTRENLSLRSLIDHSIVESFGIKGKGCITARVYPTLAIG
Sbjct: 421 SRSSLNSNDDKTTYGTFLDVDPTRENLSLRSLIDHSIVESFGIKGKGCITARVYPTLAIG 480

Query: 552 DDVSLYAFNNGTDSVTITKLTAWSMKKAHIN 583
           D V LYAFNNGTDSVTITKLTAWSMKKA IN
Sbjct: 481 DSVGLYAFNNGTDSVTITKLTAWSMKKALIN 510

BLAST of CSPI07G22800 vs. ExPASy TrEMBL
Match: A0A0A0K761 (Cell wall invertase 5 OS=Cucumis sativus OX=3659 GN=Csa_7G450770 PE=2 SV=1)

HSP 1 Score: 987.6 bits (2552), Expect = 2.2e-284
Identity = 471/582 (80.93%), Postives = 520/582 (89.35%), Query Frame = 0

Query: 5   SSSLCFLNNIFLLIFLLFGHEFLQLQALHHVYRASHRTSHFVLHQHQQQPYRTSYHFQPP 64
           S SLCF++     +  LFGH   QLQA HHV++    TS    H H +QPYRTSYHFQPP
Sbjct: 3   SISLCFIS----FLCFLFGHGVFQLQASHHVHKPQTSTS-LPYHYHHRQPYRTSYHFQPP 62

Query: 65  KNWI---NGPMIYKGIYHFFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIFPSQPSD 124
           KNWI   NGPMIYKGIYH FYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIFPSQPSD
Sbjct: 63  KNWINDPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIFPSQPSD 122

Query: 125 INGCWSGSATILPGEKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPT 184
           INGCWSGSATILPGEKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPT
Sbjct: 123 INGCWSGSATILPGEKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPT 182

Query: 185 PQNHINVSSFRDPTTAWLGRDGEWRVIIGSKIDARGLAIMYRSKDFVRWNQVDHPLRYKD 244
           PQN IN SSFRDPTTAWLG +GEWRVI+G+K+  RGLA+M++SKDF++W+QVDHPL Y D
Sbjct: 183 PQNQINASSFRDPTTAWLGPNGEWRVIVGNKVHTRGLALMFQSKDFIKWDQVDHPLHYAD 242

Query: 245 DMGMWECPDFFPVAKTGRTGVEMKVSGSHVKHILKVSLDDTKHDVYTVGTYNWKKDIYIP 304
             GMWECPDF+PVAKTGR GV+  V+G +VKH+LKVSLDDT+HDVYT+GTY+ +KDIY+P
Sbjct: 243 GSGMWECPDFYPVAKTGRRGVDTTVNGKNVKHVLKVSLDDTRHDVYTIGTYDVEKDIYVP 302

Query: 305 NKGSIEGYNGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPR 364
           NKGSIEGY+GLRYDYGKYYASKTF+D QKKRRVLWGWVNESSSV+DDIKKGWSG+Q IPR
Sbjct: 303 NKGSIEGYSGLRYDYGKYYASKTFYDGQKKRRVLWGWVNESSSVEDDIKKGWSGIQGIPR 362

Query: 365 AIWLDASGKQLIQWPIVEIQKLRNNKVKLTNEVLKKGSTIEVKGVTAAQADVEISFKVEN 424
            IWLDASGKQLIQWPI EIQKLR NKV LTN+VLKKGSTIEVKGVT +QADVE+SFKV+N
Sbjct: 363 TIWLDASGKQLIQWPIEEIQKLRKNKVTLTNKVLKKGSTIEVKGVTPSQADVEVSFKVKN 422

Query: 425 LKKAEILKPE-WKDPQLLCSQKGTSSTTEGGSVGPFGLLVLASNDLKEYTSVFFTVFKKQ 484
           LKKA+ILKP+ W++PQ+LCSQKG ++TT+GG VGPFGLLVLASND KEYTSV FT+FKKQ
Sbjct: 423 LKKAQILKPQYWENPQVLCSQKGRAATTKGG-VGPFGLLVLASNDFKEYTSVSFTIFKKQ 482

Query: 485 HKYVVLMCGDQSRSSLHSNNDKTTYGAFLDVDPTRENLSLRSLIDHSIVESFGIKGKGCI 544
            KYV LMC DQSRSSL+  NDKTTYGAFLDVD   E+LSLRSLIDHS+VESFG+KGKGCI
Sbjct: 483 SKYVALMCSDQSRSSLNPTNDKTTYGAFLDVDVDSEDLSLRSLIDHSVVESFGLKGKGCI 542

Query: 545 TARVYPTLAIGDDVSLYAFNNGTDSVTITKLTAWSMKKAHIN 583
           TARVYPTLAIGD+V LYAFNNGT+SVTITKLTAWSMKKA IN
Sbjct: 543 TARVYPTLAIGDNVGLYAFNNGTESVTITKLTAWSMKKARIN 578

BLAST of CSPI07G22800 vs. ExPASy TrEMBL
Match: A0A6J1GPU7 (beta-fructofuranosidase, insoluble isoenzyme CWINV1-like OS=Cucurbita moschata OX=3662 GN=LOC111456399 PE=3 SV=1)

HSP 1 Score: 979.9 bits (2532), Expect = 4.5e-282
Identity = 476/581 (81.93%), Postives = 519/581 (89.33%), Query Frame = 0

Query: 5   SSSLCFLNNIFLLIFLLFGHEFLQLQALHHVYRASHRTSHFVLHQHQQQPYRTSYHFQPP 64
           SSSLCF++    L FLL  H  L+LQA HHVY  + + S  +LH++ +QPYRTSYHFQPP
Sbjct: 3   SSSLCFIS---FLCFLLC-HGALKLQASHHVY--TPQPSRLLLHRY-EQPYRTSYHFQPP 62

Query: 65  KNWI---NGPMIYKGIYHFFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIFPSQPSD 124
           KNWI   NGPMIYKGIYH FYQYNPKGAVWGNIVWAHSTSTDLINW+PHD+AI+PSQPSD
Sbjct: 63  KNWINDPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWKPHDYAIYPSQPSD 122

Query: 125 INGCWSGSATILPGEKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPT 184
           INGCWSGSATILPG+KPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPT
Sbjct: 123 INGCWSGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPT 182

Query: 185 PQNHINVSSFRDPTTAWLGRDGEWRVIIGSKIDARGLAIMYRSKDFVRWNQVDHPLRYKD 244
           PQNHIN SSFRDPTTAWLG DGEWRVIIGSKIDARGLAIM++SKDFV+W+QVDHPL Y D
Sbjct: 183 PQNHINSSSFRDPTTAWLGHDGEWRVIIGSKIDARGLAIMFQSKDFVKWDQVDHPLHYAD 242

Query: 245 DMGMWECPDFFPVAKTGRTGVEMKVSGSHVKHILKVSLDDTKHDVYTVGTYNWKKDIYIP 304
             GMWECPDFFPVAK  R GV+  VSG HVKH+LKVSLDDTKHD YTVGTY+ KKDIY+P
Sbjct: 243 GTGMWECPDFFPVAKNSRAGVDTSVSGQHVKHVLKVSLDDTKHDQYTVGTYDLKKDIYVP 302

Query: 305 NKGSIEGYNGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPR 364
           +KGSIEGY GLRYDYGKYYASKTFFD QKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPR
Sbjct: 303 DKGSIEGYTGLRYDYGKYYASKTFFDGQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPR 362

Query: 365 AIWLDASGKQLIQWPIVEIQKLRNNKVKLTNEVLKKGSTIEVKGVTAAQADVEISFKVEN 424
           A+WLDASGKQLIQWPIVEIQKLR +KVKLTN+VLKKGS I VKGVTAAQADV++SFKV++
Sbjct: 363 AVWLDASGKQLIQWPIVEIQKLRKDKVKLTNKVLKKGSIIAVKGVTAAQADVDVSFKVKS 422

Query: 425 LKKAEILKPEWKDPQLLCSQKGTSSTTEGGSVGPFGLLVLASNDLKEYTSVFFTVFKKQH 484
             KA+ LK EWKDPQL+CSQ G ++    G VGPFGLLVLASN+L+EYTSVFF +FK+Q 
Sbjct: 423 FNKAQPLKAEWKDPQLVCSQMGAAAK---GGVGPFGLLVLASNNLEEYTSVFFRIFKRQD 482

Query: 485 KYVVLMCGDQSRSSLHSNNDKTTYGAFLDVDPTRENLSLRSLIDHSIVESFGIKGKGCIT 544
           KYVVLMC DQSRSSL++NNDKTTYGAFLDVDP REN+SLRSLIDHSIVESFG KGK CIT
Sbjct: 483 KYVVLMCSDQSRSSLNANNDKTTYGAFLDVDPVRENISLRSLIDHSIVESFGSKGKACIT 542

Query: 545 ARVYPTLAIGDDVSLYAFNNGTDSVTITKLTAWSMKKAHIN 583
           ARVYP LAIGD   LYAFNNGT+SVTITKL+AWSMKKA I+
Sbjct: 543 ARVYPRLAIGDKAGLYAFNNGTESVTITKLSAWSMKKAQIS 573

BLAST of CSPI07G22800 vs. NCBI nr
Match: XP_004141805.1 (beta-fructofuranosidase, insoluble isoenzyme CWINV1 [Cucumis sativus] >ALS30213.1 cell wall invertase 2 [Cucumis sativus])

HSP 1 Score: 1199.1 bits (3101), Expect = 0.0e+00
Identity = 578/585 (98.80%), Postives = 580/585 (99.15%), Query Frame = 0

Query: 1   MASYSSSLCFLNNIFLLIFLLFGHEFLQLQALHHVYRASHRTSHFVLHQHQQQPYRTSYH 60
           MASYSSSLCFLNNIFLLIFLLFGHEFLQLQALHHVYRASHRTSHFVLHQHQQQPYRTSYH
Sbjct: 1   MASYSSSLCFLNNIFLLIFLLFGHEFLQLQALHHVYRASHRTSHFVLHQHQQQPYRTSYH 60

Query: 61  FQPPKNWI---NGPMIYKGIYHFFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIFPS 120
           FQPPKNWI   NGPMIYKGIYHFFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIFPS
Sbjct: 61  FQPPKNWINDPNGPMIYKGIYHFFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIFPS 120

Query: 121 QPSDINGCWSGSATILPGEKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPL 180
           QPSDINGCWSGSATILPGEKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPL
Sbjct: 121 QPSDINGCWSGSATILPGEKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPL 180

Query: 181 MAPTPQNHINVSSFRDPTTAWLGRDGEWRVIIGSKIDARGLAIMYRSKDFVRWNQVDHPL 240
           MAPTPQNHINVSSFRDPTTAWLGRDGEWRVIIGSKIDARGLAIMYRSKDFVRWNQVDHPL
Sbjct: 181 MAPTPQNHINVSSFRDPTTAWLGRDGEWRVIIGSKIDARGLAIMYRSKDFVRWNQVDHPL 240

Query: 241 RYKDDMGMWECPDFFPVAKTGRTGVEMKVSGSHVKHILKVSLDDTKHDVYTVGTYNWKKD 300
           RYKDDMGMWECPDFFPVAKTGRTGVEMKVSGSHVKHILKVSLDDTKHDVYTVGTYNWKKD
Sbjct: 241 RYKDDMGMWECPDFFPVAKTGRTGVEMKVSGSHVKHILKVSLDDTKHDVYTVGTYNWKKD 300

Query: 301 IYIPNKGSIEGYNGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQ 360
           IYIPNKGSIEGYNGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQ
Sbjct: 301 IYIPNKGSIEGYNGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQ 360

Query: 361 AIPRAIWLDASGKQLIQWPIVEIQKLRNNKVKLTNEVLKKGSTIEVKGVTAAQADVEISF 420
           AIPRAIWLDASGKQLIQWPIVEIQKLRNNKVKLTNEVLKKGSTIEVKGVTAAQADVEISF
Sbjct: 361 AIPRAIWLDASGKQLIQWPIVEIQKLRNNKVKLTNEVLKKGSTIEVKGVTAAQADVEISF 420

Query: 421 KVENLKKAEILKPEWKDPQLLCSQKGTSSTTEGGSVGPFGLLVLASNDLKEYTSVFFTVF 480
           KVENLKKAEILKPEWKDPQLLCSQKGTSSTTEGGSVGPFGLLVLASNDLKEYTSVFFTVF
Sbjct: 421 KVENLKKAEILKPEWKDPQLLCSQKGTSSTTEGGSVGPFGLLVLASNDLKEYTSVFFTVF 480

Query: 481 KKQHKYVVLMCGDQSRSSLHSNNDKTTYGAFLDVDPTRENLSLRSLIDHSIVESFGIKGK 540
           KK HKYVVLMC DQSRSSLHSNNDKTTYGAFLDVDPTREN+SLRSLIDHSIVESFGIKGK
Sbjct: 481 KKPHKYVVLMCSDQSRSSLHSNNDKTTYGAFLDVDPTRENISLRSLIDHSIVESFGIKGK 540

Query: 541 GCITARVYPTLAIGDDVSLYAFNNGTDSVTITKLTAWSMKKAHIN 583
           GCITARVYPTLAIGD+VSLYAFNNGTDSVTITKLTAWSMKKAHIN
Sbjct: 541 GCITARVYPTLAIGDNVSLYAFNNGTDSVTITKLTAWSMKKAHIN 585

BLAST of CSPI07G22800 vs. NCBI nr
Match: KAE8646511.1 (hypothetical protein Csa_015867 [Cucumis sativus])

HSP 1 Score: 1060.8 bits (2742), Expect = 4.1e-306
Identity = 503/586 (85.84%), Postives = 546/586 (93.17%), Query Frame = 0

Query: 1   MASYSSSLCFLNNIFLLIFLLFGHEFLQLQALHHVYRASHRTSHFVLHQHQQQPYRTSYH 60
           MASYSSSLCFLNNIFLLIFLLFGHEFLQLQALHHVYRASHRTSHFVLHQHQQQPYRTSYH
Sbjct: 1   MASYSSSLCFLNNIFLLIFLLFGHEFLQLQALHHVYRASHRTSHFVLHQHQQQPYRTSYH 60

Query: 61  FQPPKNWI---NGPMIYKGIYHFFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIFPS 120
           FQPPKNWI   NGPMIYKGIYH FYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIFPS
Sbjct: 61  FQPPKNWINDPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIFPS 120

Query: 121 QPSDINGCWSGSATILPGEKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPL 180
           QPSDINGCWSGSATILPGEKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPL
Sbjct: 121 QPSDINGCWSGSATILPGEKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPL 180

Query: 181 MAPTPQNHINVSSFRDPTTAWLGRDGEWRVIIGSKIDARGLAIMYRSKDFVRWNQVDHPL 240
           MAPTPQN IN SSFRDPTTAWLG +GEWRVI+G+K+  RGLA+M++SKDF++W+QVDHPL
Sbjct: 181 MAPTPQNQINASSFRDPTTAWLGPNGEWRVIVGNKVHTRGLALMFQSKDFIKWDQVDHPL 240

Query: 241 RYKDDMGMWECPDFFPVAKTGRTGVEMKVSGSHVKHILKVSLDDTKHDVYTVGTYNWKKD 300
            Y D  GMWECPDF+PVAKTGR GV+  V+G +VKH+LKVSLDDT+HDVYT+GTY+ +KD
Sbjct: 241 HYADGSGMWECPDFYPVAKTGRRGVDTTVNGKNVKHVLKVSLDDTRHDVYTIGTYDVEKD 300

Query: 301 IYIPNKGSIEGYNGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQ 360
           IY+PNKGSIEGY+GLRYDYGKYYASKTF+D QKKRRVLWGWVNESSSV+DDIKKGWSG+Q
Sbjct: 301 IYVPNKGSIEGYSGLRYDYGKYYASKTFYDGQKKRRVLWGWVNESSSVEDDIKKGWSGIQ 360

Query: 361 AIPRAIWLDASGKQLIQWPIVEIQKLRNNKVKLTNEVLKKGSTIEVKGVTAAQADVEISF 420
            IPR IWLDASGKQLIQWPI EIQKLR NKV LTN+VLKKGSTIEVKGVT +QADVE+SF
Sbjct: 361 GIPRTIWLDASGKQLIQWPIEEIQKLRKNKVTLTNKVLKKGSTIEVKGVTPSQADVEVSF 420

Query: 421 KVENLKKAEILKPE-WKDPQLLCSQKGTSSTTEGGSVGPFGLLVLASNDLKEYTSVFFTV 480
           KV+NLKKA+ILKP+ W++PQ+LCSQKG ++TT+GG VGPFGLLVLASND KEYTSV FT+
Sbjct: 421 KVKNLKKAQILKPQYWENPQVLCSQKGRAATTKGG-VGPFGLLVLASNDFKEYTSVSFTI 480

Query: 481 FKKQHKYVVLMCGDQSRSSLHSNNDKTTYGAFLDVDPTRENLSLRSLIDHSIVESFGIKG 540
           FKKQ KYV LMC DQSRSSL+  NDKTTYGAFLDVD   E+LSLRSLIDHS+VESFG+KG
Sbjct: 481 FKKQSKYVALMCSDQSRSSLNPTNDKTTYGAFLDVDVDSEDLSLRSLIDHSVVESFGLKG 540

Query: 541 KGCITARVYPTLAIGDDVSLYAFNNGTDSVTITKLTAWSMKKAHIN 583
           KGCITARVYPTLAIGD+V LYAFNNGT+SVTITKLTAWSMKKA IN
Sbjct: 541 KGCITARVYPTLAIGDNVGLYAFNNGTESVTITKLTAWSMKKARIN 585

BLAST of CSPI07G22800 vs. NCBI nr
Match: XP_038898203.1 (beta-fructofuranosidase, insoluble isoenzyme CWINV1-like [Benincasa hispida])

HSP 1 Score: 998.8 bits (2581), Expect = 1.9e-287
Identity = 486/585 (83.08%), Postives = 522/585 (89.23%), Query Frame = 0

Query: 1   MASYSSSLCFLNNIFLLIFLLFGHEFLQLQALHHVYRASHRTSHFVLHQHQQQPYRTSYH 60
           MAS+  SLCF++     +  LFGH  LQLQA HHVYRA    S   L  H QQPYRTSYH
Sbjct: 1   MASF--SLCFIS----FLCFLFGHGVLQLQASHHVYRAQLSNS---LPHHYQQPYRTSYH 60

Query: 61  FQPPKNWI---NGPMIYKGIYHFFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIFPS 120
           FQPPKNWI   NGPMIYKGIYH FYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAI+PS
Sbjct: 61  FQPPKNWINDPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIYPS 120

Query: 121 QPSDINGCWSGSATILPGEKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPL 180
           QPSDINGCWSGSATILPG+KPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPL
Sbjct: 121 QPSDINGCWSGSATILPGDKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPL 180

Query: 181 MAPTPQNHINVSSFRDPTTAWLGRDGEWRVIIGSKIDARGLAIMYRSKDFVRWNQVDHPL 240
           MAPTPQNHIN SSFRDPTTAWLG DGEWRVIIGSKI+ARGLAIMY+SKDFV+W+QVDHPL
Sbjct: 181 MAPTPQNHINSSSFRDPTTAWLGPDGEWRVIIGSKINARGLAIMYQSKDFVKWDQVDHPL 240

Query: 241 RYKDDMGMWECPDFFPVAKTGRTGVEMKVSGSHVKHILKVSLDDTKHDVYTVGTYNWKKD 300
            Y D  GMWECPDFFPVAKTGR GV+  VSG HVKH+LKVSLDDTKHD YTVG Y+ +KD
Sbjct: 241 HYADGTGMWECPDFFPVAKTGRKGVDTSVSGGHVKHVLKVSLDDTKHDHYTVGNYDLEKD 300

Query: 301 IYIPNKGSIEGYNGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQ 360
           IY+PNKGSIEGY GLRYDYGKYYASKTF+D QKKRRVLWGWVNESSSV+DDIKKGWSG+Q
Sbjct: 301 IYVPNKGSIEGYMGLRYDYGKYYASKTFYDGQKKRRVLWGWVNESSSVEDDIKKGWSGIQ 360

Query: 361 AIPRAIWLDASGKQLIQWPIVEIQKLRNNKVKLTNEVLKKGSTIEVKGVTAAQADVEISF 420
           AIPR IWLDASGKQLIQWPI EIQKLR +KVKLTN+VLKKGS IEVKGVTAAQADVE+SF
Sbjct: 361 AIPRTIWLDASGKQLIQWPIEEIQKLRKDKVKLTNKVLKKGSLIEVKGVTAAQADVEVSF 420

Query: 421 KVENLKKAEILKPEWKDPQLLCSQKGTSSTTEGGSVGPFGLLVLASNDLKEYTSVFFTVF 480
           KV+N KKA+ILKPEWKDPQLLCSQKG  +TT+GG VG FGLLVLAS DLKEYTS+ FT+F
Sbjct: 421 KVKNFKKAQILKPEWKDPQLLCSQKG--ATTKGG-VGSFGLLVLASKDLKEYTSISFTIF 480

Query: 481 KKQHKYVVLMCGDQSRSSLHSNNDKTTYGAFLDVDPTRENLSLRSLIDHSIVESFGIKGK 540
           K+Q KY+VLMC DQSRSSL+ NNDKTTYGAFL+VDP  E+LSLRSLIDHS+VESFG KGK
Sbjct: 481 KRQGKYIVLMCSDQSRSSLNPNNDKTTYGAFLNVDPVSEDLSLRSLIDHSVVESFGSKGK 540

Query: 541 GCITARVYPTLAIGDDVSLYAFNNGTDSVTITKLTAWSMKKAHIN 583
            CITARVYPTLAIGD+V LY FNNGT+SVTITKL AWS+KKA IN
Sbjct: 541 ACITARVYPTLAIGDNVGLYVFNNGTESVTITKLGAWSLKKAQIN 573

BLAST of CSPI07G22800 vs. NCBI nr
Match: KAA0059425.1 (beta-fructofuranosidase, insoluble isoenzyme CWINV1-like [Cucumis melo var. makuwa] >TYK03900.1 beta-fructofuranosidase, insoluble isoenzyme CWINV1-like [Cucumis melo var. makuwa])

HSP 1 Score: 998.0 bits (2579), Expect = 3.3e-287
Identity = 473/511 (92.56%), Postives = 489/511 (95.69%), Query Frame = 0

Query: 72  MIYKGIYHFFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIFPSQPSDINGCWSGSAT 131
           MIYKGIYH FYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAI+PSQPSDINGCWSGSAT
Sbjct: 1   MIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIYPSQPSDINGCWSGSAT 60

Query: 132 ILPGEKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNHINVSSF 191
           ILPGEKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNHIN SSF
Sbjct: 61  ILPGEKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQNHINASSF 120

Query: 192 RDPTTAWLGRDGEWRVIIGSKIDARGLAIMYRSKDFVRWNQVDHPLRYKDDMGMWECPDF 251
           RDPTTAW+G DGEWRVI+GSK+DARGLA+MYRSKDFV+WNQVDHPL YKD MGMWECPDF
Sbjct: 121 RDPTTAWIGPDGEWRVIVGSKLDARGLAVMYRSKDFVKWNQVDHPLHYKDGMGMWECPDF 180

Query: 252 FPVAKTGRTGVEMKVSGSHVKHILKVSLDDTKHDVYTVGTYNWKKDIYIPNKGSIEGYNG 311
           FPVAKTGR GVE +V GS VKH+LKVSLDDTKHDVYTVGTYNW+KDIY+PNKGSIE YNG
Sbjct: 181 FPVAKTGRKGVETRVYGSQVKHVLKVSLDDTKHDVYTVGTYNWQKDIYVPNKGSIESYNG 240

Query: 312 LRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRAIWLDASGKQ 371
           LRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRA+WLDASGKQ
Sbjct: 241 LRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPRAVWLDASGKQ 300

Query: 372 LIQWPIVEIQKLRNNKVKLTNEVLKKGSTIEVKGVTAAQADVEISFKVENLKKAEILKPE 431
           LIQWP+VEIQKLRNNKVKLTNEVLKKGS +EVKGVTAAQADVEISFKVEN KKAEILKPE
Sbjct: 301 LIQWPVVEIQKLRNNKVKLTNEVLKKGSIVEVKGVTAAQADVEISFKVENFKKAEILKPE 360

Query: 432 WKDPQLLCSQKGTSSTTEGGSVGPFGLLVLASNDLKEYTSVFFTVFKKQHKYVVLMCGDQ 491
           WKDPQLLCSQKGT STT+ GSVGPFGLLVLASNDLKEYTSV F VFK QHKYVVLMC DQ
Sbjct: 361 WKDPQLLCSQKGT-STTKSGSVGPFGLLVLASNDLKEYTSVSFRVFKGQHKYVVLMCSDQ 420

Query: 492 SRSSLHSNNDKTTYGAFLDVDPTRENLSLRSLIDHSIVESFGIKGKGCITARVYPTLAIG 551
           SRSSL+SN+DKTTYG FLDVDPTRENLSLRSLIDHSIVESFGIKGKGCITARVYPTLAIG
Sbjct: 421 SRSSLNSNDDKTTYGTFLDVDPTRENLSLRSLIDHSIVESFGIKGKGCITARVYPTLAIG 480

Query: 552 DDVSLYAFNNGTDSVTITKLTAWSMKKAHIN 583
           D V LYAFNNGTDSVTITKLTAWSMKKA IN
Sbjct: 481 DSVGLYAFNNGTDSVTITKLTAWSMKKALIN 510

BLAST of CSPI07G22800 vs. NCBI nr
Match: XP_004141685.2 (beta-fructofuranosidase, insoluble isoenzyme CWINV1 [Cucumis sativus] >ALS30216.1 cell wall invertase 5 [Cucumis sativus])

HSP 1 Score: 987.6 bits (2552), Expect = 4.5e-284
Identity = 471/582 (80.93%), Postives = 520/582 (89.35%), Query Frame = 0

Query: 5   SSSLCFLNNIFLLIFLLFGHEFLQLQALHHVYRASHRTSHFVLHQHQQQPYRTSYHFQPP 64
           S SLCF++     +  LFGH   QLQA HHV++    TS    H H +QPYRTSYHFQPP
Sbjct: 3   SISLCFIS----FLCFLFGHGVFQLQASHHVHKPQTSTS-LPYHYHHRQPYRTSYHFQPP 62

Query: 65  KNWI---NGPMIYKGIYHFFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIFPSQPSD 124
           KNWI   NGPMIYKGIYH FYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIFPSQPSD
Sbjct: 63  KNWINDPNGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIFPSQPSD 122

Query: 125 INGCWSGSATILPGEKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPT 184
           INGCWSGSATILPGEKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPT
Sbjct: 123 INGCWSGSATILPGEKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPT 182

Query: 185 PQNHINVSSFRDPTTAWLGRDGEWRVIIGSKIDARGLAIMYRSKDFVRWNQVDHPLRYKD 244
           PQN IN SSFRDPTTAWLG +GEWRVI+G+K+  RGLA+M++SKDF++W+QVDHPL Y D
Sbjct: 183 PQNQINASSFRDPTTAWLGPNGEWRVIVGNKVHTRGLALMFQSKDFIKWDQVDHPLHYAD 242

Query: 245 DMGMWECPDFFPVAKTGRTGVEMKVSGSHVKHILKVSLDDTKHDVYTVGTYNWKKDIYIP 304
             GMWECPDF+PVAKTGR GV+  V+G +VKH+LKVSLDDT+HDVYT+GTY+ +KDIY+P
Sbjct: 243 GSGMWECPDFYPVAKTGRRGVDTTVNGKNVKHVLKVSLDDTRHDVYTIGTYDVEKDIYVP 302

Query: 305 NKGSIEGYNGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPR 364
           NKGSIEGY+GLRYDYGKYYASKTF+D QKKRRVLWGWVNESSSV+DDIKKGWSG+Q IPR
Sbjct: 303 NKGSIEGYSGLRYDYGKYYASKTFYDGQKKRRVLWGWVNESSSVEDDIKKGWSGIQGIPR 362

Query: 365 AIWLDASGKQLIQWPIVEIQKLRNNKVKLTNEVLKKGSTIEVKGVTAAQADVEISFKVEN 424
            IWLDASGKQLIQWPI EIQKLR NKV LTN+VLKKGSTIEVKGVT +QADVE+SFKV+N
Sbjct: 363 TIWLDASGKQLIQWPIEEIQKLRKNKVTLTNKVLKKGSTIEVKGVTPSQADVEVSFKVKN 422

Query: 425 LKKAEILKPE-WKDPQLLCSQKGTSSTTEGGSVGPFGLLVLASNDLKEYTSVFFTVFKKQ 484
           LKKA+ILKP+ W++PQ+LCSQKG ++TT+GG VGPFGLLVLASND KEYTSV FT+FKKQ
Sbjct: 423 LKKAQILKPQYWENPQVLCSQKGRAATTKGG-VGPFGLLVLASNDFKEYTSVSFTIFKKQ 482

Query: 485 HKYVVLMCGDQSRSSLHSNNDKTTYGAFLDVDPTRENLSLRSLIDHSIVESFGIKGKGCI 544
            KYV LMC DQSRSSL+  NDKTTYGAFLDVD   E+LSLRSLIDHS+VESFG+KGKGCI
Sbjct: 483 SKYVALMCSDQSRSSLNPTNDKTTYGAFLDVDVDSEDLSLRSLIDHSVVESFGLKGKGCI 542

Query: 545 TARVYPTLAIGDDVSLYAFNNGTDSVTITKLTAWSMKKAHIN 583
           TARVYPTLAIGD+V LYAFNNGT+SVTITKLTAWSMKKA IN
Sbjct: 543 TARVYPTLAIGDNVGLYAFNNGTESVTITKLTAWSMKKARIN 578

BLAST of CSPI07G22800 vs. TAIR 10
Match: AT3G13790.2 (Glycosyl hydrolases family 32 protein )

HSP 1 Score: 775.0 bits (2000), Expect = 4.2e-224
Identity = 375/585 (64.10%), Postives = 449/585 (76.75%), Query Frame = 0

Query: 5   SSSLCFLNNIFLLIFLLFGHEFLQLQALHHVYRASHRTSHFVLHQHQQQPYRTSYHFQPP 64
           +  +C    ++LL+ LL G+  + L+A HHVY+   ++++        QPYRT +HFQPP
Sbjct: 2   TKEVCSNIGLWLLLTLLIGNYVVNLEASHHVYKRLTQSTN-TKSPSVNQPYRTGFHFQPP 61

Query: 65  KNWINGPMIYKGIYHFFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIFPSQPSDING 124
           KNW+NGPMIYKGIYH FYQ+NPKGAVWGNIVWAHSTSTDLINW+PH  AIFPS P DING
Sbjct: 62  KNWMNGPMIYKGIYHLFYQWNPKGAVWGNIVWAHSTSTDLINWDPHPPAIFPSAPFDING 121

Query: 125 CWSGSATILPGEKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTPQN 184
           CWSGSATILP  KP ILYTGI+PKNQQVQN+A PKNLSDPYLREW KSP NPLMAP   N
Sbjct: 122 CWSGSATILPNGKPVILYTGIDPKNQQVQNIAEPKNLSDPYLREWKKSPLNPLMAPDAVN 181

Query: 185 HINVSSFRDPTTAWLGRDGEWRVIIGSKIDARGLAIMYRSKDFVRWNQVDHPLRYKDDMG 244
            IN SSFRDPTTAWLG+D +WRVIIGSKI  RGLAI Y SKDF++W +   PL Y D  G
Sbjct: 182 GINASSFRDPTTAWLGQDKKWRVIIGSKIHRRGLAITYTSKDFLKWEKSPEPLHYDDGSG 241

Query: 245 MWECPDFFPVAKTGRTGVEMKVSGSH---VKHILKVSLDDTKHDVYTVGTYNWKKDIYIP 304
           MWECPDFFPV + G  GVE    G     +KH+LK+SLDDTKHD YT+GTY+  KD ++P
Sbjct: 242 MWECPDFFPVTRFGSNGVETSSFGEPNEILKHVLKISLDDTKHDYYTIGTYDRVKDKFVP 301

Query: 305 NKGSIEGYNGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIPR 364
           + G        RYDYGKYYASKTFFDS K RR+LWGW NESSSV+DD++KGWSG+Q IPR
Sbjct: 302 DNGFKMDGTAPRYDYGKYYASKTFFDSAKNRRILWGWTNESSSVEDDVEKGWSGIQTIPR 361

Query: 365 AIWLDASGKQLIQWPIVEIQKLRNNKVK-LTNEVLKKGSTIEVKGVTAAQADVEISFKVE 424
            IWLD SGKQLIQWP+ E+++LR  +VK L N+VLK GS +EV GVTAAQADVE+ FKV 
Sbjct: 362 KIWLDRSGKQLIQWPVREVERLRTKQVKNLRNKVLKSGSRLEVYGVTAAQADVEVLFKVR 421

Query: 425 NLKKAEILKPEWKDPQLLCSQKGTSSTTEGGSVGPFGLLVLASNDLKEYTSVFFTVFK-- 484
           +L+KA++++P W DPQL+CS+   S  +    +GPFGL+VLAS +L+EYTSV+F +FK  
Sbjct: 422 DLEKADVIEPSWTDPQLICSKMNVSVKS---GLGPFGLMVLASKNLEEYTSVYFRIFKAR 481

Query: 485 -KQHKYVVLMCGDQSRSSLHSNNDKTTYGAFLDVDPTRENLSLRSLIDHSIVESFGIKGK 544
              +KYVVLMC DQSRSSL  +NDKTTYGAF+D++P  + LSLR+LIDHS+VESFG KG+
Sbjct: 482 QNSNKYVVLMCSDQSRSSLKEDNDKTTYGAFVDINP-HQPLSLRALIDHSVVESFGGKGR 541

Query: 545 GCITARVYPTLAIGDDVSLYAFNNGTDSVTITKLTAWSMKKAHIN 583
            CIT+RVYP LAIG    L+AFN G  SV +  L AWSM  A I+
Sbjct: 542 ACITSRVYPKLAIGKSSHLFAFNYGYQSVDVLNLNAWSMNSAQIS 581

BLAST of CSPI07G22800 vs. TAIR 10
Match: AT3G13790.1 (Glycosyl hydrolases family 32 protein )

HSP 1 Score: 769.6 bits (1986), Expect = 1.8e-222
Identity = 375/588 (63.78%), Postives = 449/588 (76.36%), Query Frame = 0

Query: 5   SSSLCFLNNIFLLIFLLFGHEFLQLQALHHVYRASHRTSHFVLHQHQQQPYRTSYHFQPP 64
           +  +C    ++LL+ LL G+  + L+A HHVY+   ++++        QPYRT +HFQPP
Sbjct: 2   TKEVCSNIGLWLLLTLLIGNYVVNLEASHHVYKRLTQSTN-TKSPSVNQPYRTGFHFQPP 61

Query: 65  KNWI---NGPMIYKGIYHFFYQYNPKGAVWGNIVWAHSTSTDLINWEPHDHAIFPSQPSD 124
           KNW+   NGPMIYKGIYH FYQ+NPKGAVWGNIVWAHSTSTDLINW+PH  AIFPS P D
Sbjct: 62  KNWMNDPNGPMIYKGIYHLFYQWNPKGAVWGNIVWAHSTSTDLINWDPHPPAIFPSAPFD 121

Query: 125 INGCWSGSATILPGEKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPT 184
           INGCWSGSATILP  KP ILYTGI+PKNQQVQN+A PKNLSDPYLREW KSP NPLMAP 
Sbjct: 122 INGCWSGSATILPNGKPVILYTGIDPKNQQVQNIAEPKNLSDPYLREWKKSPLNPLMAPD 181

Query: 185 PQNHINVSSFRDPTTAWLGRDGEWRVIIGSKIDARGLAIMYRSKDFVRWNQVDHPLRYKD 244
             N IN SSFRDPTTAWLG+D +WRVIIGSKI  RGLAI Y SKDF++W +   PL Y D
Sbjct: 182 AVNGINASSFRDPTTAWLGQDKKWRVIIGSKIHRRGLAITYTSKDFLKWEKSPEPLHYDD 241

Query: 245 DMGMWECPDFFPVAKTGRTGVEMKVSGSH---VKHILKVSLDDTKHDVYTVGTYNWKKDI 304
             GMWECPDFFPV + G  GVE    G     +KH+LK+SLDDTKHD YT+GTY+  KD 
Sbjct: 242 GSGMWECPDFFPVTRFGSNGVETSSFGEPNEILKHVLKISLDDTKHDYYTIGTYDRVKDK 301

Query: 305 YIPNKGSIEGYNGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQA 364
           ++P+ G        RYDYGKYYASKTFFDS K RR+LWGW NESSSV+DD++KGWSG+Q 
Sbjct: 302 FVPDNGFKMDGTAPRYDYGKYYASKTFFDSAKNRRILWGWTNESSSVEDDVEKGWSGIQT 361

Query: 365 IPRAIWLDASGKQLIQWPIVEIQKLRNNKVK-LTNEVLKKGSTIEVKGVTAAQADVEISF 424
           IPR IWLD SGKQLIQWP+ E+++LR  +VK L N+VLK GS +EV GVTAAQADVE+ F
Sbjct: 362 IPRKIWLDRSGKQLIQWPVREVERLRTKQVKNLRNKVLKSGSRLEVYGVTAAQADVEVLF 421

Query: 425 KVENLKKAEILKPEWKDPQLLCSQKGTSSTTEGGSVGPFGLLVLASNDLKEYTSVFFTVF 484
           KV +L+KA++++P W DPQL+CS+   S  +    +GPFGL+VLAS +L+EYTSV+F +F
Sbjct: 422 KVRDLEKADVIEPSWTDPQLICSKMNVSVKS---GLGPFGLMVLASKNLEEYTSVYFRIF 481

Query: 485 K---KQHKYVVLMCGDQSRSSLHSNNDKTTYGAFLDVDPTRENLSLRSLIDHSIVESFGI 544
           K     +KYVVLMC DQSRSSL  +NDKTTYGAF+D++P  + LSLR+LIDHS+VESFG 
Sbjct: 482 KARQNSNKYVVLMCSDQSRSSLKEDNDKTTYGAFVDINP-HQPLSLRALIDHSVVESFGG 541

Query: 545 KGKGCITARVYPTLAIGDDVSLYAFNNGTDSVTITKLTAWSMKKAHIN 583
           KG+ CIT+RVYP LAIG    L+AFN G  SV +  L AWSM  A I+
Sbjct: 542 KGRACITSRVYPKLAIGKSSHLFAFNYGYQSVDVLNLNAWSMNSAQIS 584

BLAST of CSPI07G22800 vs. TAIR 10
Match: AT1G55120.1 (beta-fructofuranosidase 5 )

HSP 1 Score: 716.5 bits (1848), Expect = 1.8e-206
Identity = 344/547 (62.89%), Postives = 415/547 (75.87%), Query Frame = 0

Query: 48  HQHQQQPYRTSYHFQPPKNWI---NGPMIYKGIYHFFYQYNPKGAVWG-NIVWAHSTSTD 107
           HQ   QPYRT YHFQP KNW+   NGPMIYKGIYH FYQYNP GAVW   IVW HSTS D
Sbjct: 31  HQDLNQPYRTGYHFQPLKNWMNDPNGPMIYKGIYHLFYQYNPYGAVWDVRIVWGHSTSVD 90

Query: 108 LINWEPHDHAIFPSQPSDINGCWSGSATILPGEKPAILYTGINPKNQQVQNLAVPKNLSD 167
           L+NW     A  PSQPSDINGCWSGS TILP  KP ILYTGI+    QVQN+AVP N+SD
Sbjct: 91  LVNWISQPPAFNPSQPSDINGCWSGSVTILPNGKPVILYTGIDQNKGQVQNVAVPVNISD 150

Query: 168 PYLREWVKSPKNPLMAPTPQNHINVSSFRDPTTAWLGRDGEWRVIIGSKI-DARGLAIMY 227
           PYLREW K P+NPLM     N IN   FRDPTTAWLGRDGEWRVI+GS   D RGLAI+Y
Sbjct: 151 PYLREWSKPPQNPLMTTNAVNGINPDRFRDPTTAWLGRDGEWRVIVGSSTDDRRGLAILY 210

Query: 228 RSKDFVRWNQVDHPLRYKDDMGMWECPDFFPVAKTGRTGVEMKVSGSH-VKHILKVSLDD 287
           +S+DF  W Q   PL Y+D  GMWECPDFFPV+ TG  GVE    G + +KH+LKVSL +
Sbjct: 211 KSRDFFNWTQSMKPLHYEDLTGMWECPDFFPVSITGSDGVETSSVGENGIKHVLKVSLIE 270

Query: 288 TKHDVYTVGTYNWKKDIYIPNKGSIEGYNGLRYDYGKYYASKTFFDSQKKRRVLWGWVNE 347
           T HD YT+G+Y+ +KD+Y+P+ G ++  +  R DYGKYYASKTF+D  KKRR+LWGWVNE
Sbjct: 271 TLHDYYTIGSYDREKDVYVPDLGFVQNESAPRLDYGKYYASKTFYDDVKKRRILWGWVNE 330

Query: 348 SSSVDDDIKKGWSGVQAIPRAIWLDASGKQLIQWPIVEIQKLRNNKVKLTNEVLKKGSTI 407
           SS   DDI+KGWSG+Q+ PR IWLD SGK+L+QWPI EI+ LR  +V    +VLK GST+
Sbjct: 331 SSPAKDDIEKGWSGLQSFPRKIWLDESGKELLQWPIEEIETLRGQQVNWQKKVLKAGSTL 390

Query: 408 EVKGVTAAQADVEISFKVENLKKAEILKPEWKDPQLLCSQKGTSSTTEGGSVGPFGLLVL 467
           +V GVTAAQADVE+SFKV+ L+KA++++P W DPQ +CSQ G  S   G  +GPFGL+VL
Sbjct: 391 QVHGVTAAQADVEVSFKVKELEKADVIEPSWTDPQKICSQ-GDLSVMSG--LGPFGLMVL 450

Query: 468 ASNDLKEYTSVFFTVFK------KQHKYVVLMCGDQSRSSLHSNNDKTTYGAFLDVDPTR 527
           ASND++EYTSV+F +FK      K+ KYVVLMC DQSRSSL+  NDK+T+GAF+ +DP+ 
Sbjct: 451 ASNDMEEYTSVYFRIFKSNDDTNKKTKYVVLMCSDQSRSSLNDENDKSTFGAFVAIDPSH 510

Query: 528 ENLSLRSLIDHSIVESFGIKGKGCITARVYPTLAIGDDVSLYAFNNGTDSVTITKLTAWS 583
           + +SLR+LIDHSIVES+G  G+ CIT+RVYP LAIG++ +L+ FN GT SV I  L+AWS
Sbjct: 511 QTISLRTLIDHSIVESYGGGGRTCITSRVYPKLAIGENANLFVFNKGTQSVDILTLSAWS 570

BLAST of CSPI07G22800 vs. TAIR 10
Match: AT3G13784.1 (cell wall invertase 5 )

HSP 1 Score: 690.3 bits (1780), Expect = 1.4e-198
Identity = 329/537 (61.27%), Postives = 406/537 (75.61%), Query Frame = 0

Query: 51  QQQPYRTSYHFQPPKNWINGPMIYKGIYHFFYQYNPKGAVW--GNIVWAHSTSTDLINWE 110
           + QPYRT YHFQPPKNW+NGPMIYKGIYH FYQ+N  GAV      VW H+TSTDLINW 
Sbjct: 38  KNQPYRTGYHFQPPKNWMNGPMIYKGIYHLFYQWNQNGAVMDVNKTVWGHATSTDLINWI 97

Query: 111 PHDHAIFPSQPSDINGCWSGSATILPGEKPAILYTGINPKNQQVQNLAVPKNLSDPYLRE 170
               AI PS+PSDINGCWSGS TILP  KP ILYTG +  N+QVQNL  PKNL+DPYLR 
Sbjct: 98  TLSPAIRPSRPSDINGCWSGSVTILPNGKPVILYTGNDRYNRQVQNLVKPKNLTDPYLRH 157

Query: 171 WVKSPKNPLMAPTPQNHINVSSFRDPTTAWLGRDGEWRVIIGSKIDARGLAIMYRSKDFV 230
           W KSP+NPL+ P+P NHIN S+FRDPTTAW GRDG WR+  GS+   RGLAI++ SKDFV
Sbjct: 158 WTKSPENPLVTPSPVNHINSSAFRDPTTAWFGRDGRWRITTGSQEGRRGLAILHTSKDFV 217

Query: 231 RWNQVDHPLRYKDDMGMWECPDFFPVAKTGRTGVEMKV-SGSHVKHILKVSLDDTKHDVY 290
            W Q   PL Y D  GMWECPDFFPVA+T   G++    SG  VKH+LKVSL DT +D Y
Sbjct: 218 IWKQSPKPLHYHDGTGMWECPDFFPVARTDSRGLDTSFSSGPMVKHVLKVSLTDTFNDYY 277

Query: 291 TVGTYNWKKDIYIPNKGSIEGYNGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDD 350
           T+GTY+  +D+Y+P+KG ++     RYDYGK+YASKTF+DS  +RR+LWGWVNESS   D
Sbjct: 278 TIGTYDEVRDVYVPDKGFVQDETAPRYDYGKFYASKTFYDSVNQRRILWGWVNESSPEKD 337

Query: 351 DIKKGWSGVQAIPRAIWLDASGKQLIQWPIVEIQKLRNNKVKLTNEVLKKGSTIEVKGVT 410
           +IKKGW+G+QAIPR +WLD SGK+L+QWP+ EI++LR  +VK  N++LK GS +EV GVT
Sbjct: 338 NIKKGWAGLQAIPRKVWLDESGKRLVQWPVKEIERLRTTQVKWGNKLLKGGSVMEVHGVT 397

Query: 411 AAQADVEISFKVE--NLKKAEILKPEWKDPQLLCSQKGTSSTTEGGSVGPFGLLVLASND 470
           A QADVE+ FKV   +L+KA++++P W DPQL+CSQ+  SS      +GPFGL+VLAS +
Sbjct: 398 APQADVEVFFKVSGFDLEKADVIEPGWTDPQLICSQRNASS-----GLGPFGLMVLASKN 457

Query: 471 LKEYTSVFFTVFK---KQHKYVVLMCGDQSRSSLHSNNDKTTYGAFLDVDPTRENLSLRS 530
           ++EYTSV   +F+      ++VV+MC DQS SSL   NDKTTYGAFLD+ P  + +SLR+
Sbjct: 458 MEEYTSVNIRIFRAGENSKEHVVVMCSDQSTSSLEKGNDKTTYGAFLDISP-YQPISLRT 517

Query: 531 LIDHSIVESFGIKGKGCITARVYPTLAIGDDVSLYAFNNGTDSVTITKLTAWSMKKA 580
           LID SIVESFG KGK CIT+RVYP LAIG+   L+AFN G+ +V +  L+AWSMK +
Sbjct: 518 LIDKSIVESFGGKGKTCITSRVYPKLAIGERTHLFAFNKGSQNVNVLSLSAWSMKSS 568

BLAST of CSPI07G22800 vs. TAIR 10
Match: AT1G55120.3 (beta-fructofuranosidase 5 )

HSP 1 Score: 689.5 bits (1778), Expect = 2.3e-198
Identity = 329/528 (62.31%), Postives = 401/528 (75.95%), Query Frame = 0

Query: 64  PKNWINGPMIYKGIYHFFYQYNPKGAVWG-NIVWAHSTSTDLINWEPHDHAIFPSQPSDI 123
           P   ++GPMIYKGIYH FYQYNP GAVW   IVW HSTS DL+NW     A  PSQPSDI
Sbjct: 35  PNVSVSGPMIYKGIYHLFYQYNPYGAVWDVRIVWGHSTSVDLVNWISQPPAFNPSQPSDI 94

Query: 124 NGCWSGSATILPGEKPAILYTGINPKNQQVQNLAVPKNLSDPYLREWVKSPKNPLMAPTP 183
           NGCWSGS TILP  KP ILYTGI+    QVQN+AVP N+SDPYLREW K P+NPLM    
Sbjct: 95  NGCWSGSVTILPNGKPVILYTGIDQNKGQVQNVAVPVNISDPYLREWSKPPQNPLMTTNA 154

Query: 184 QNHINVSSFRDPTTAWLGRDGEWRVIIGSKI-DARGLAIMYRSKDFVRWNQVDHPLRYKD 243
            N IN   FRDPTTAWLGRDGEWRVI+GS   D RGLAI+Y+S+DF  W Q   PL Y+D
Sbjct: 155 VNGINPDRFRDPTTAWLGRDGEWRVIVGSSTDDRRGLAILYKSRDFFNWTQSMKPLHYED 214

Query: 244 DMGMWECPDFFPVAKTGRTGVEMKVSGSH-VKHILKVSLDDTKHDVYTVGTYNWKKDIYI 303
             GMWECPDFFPV+ TG  GVE    G + +KH+LKVSL +T HD YT+G+Y+ +KD+Y+
Sbjct: 215 LTGMWECPDFFPVSITGSDGVETSSVGENGIKHVLKVSLIETLHDYYTIGSYDREKDVYV 274

Query: 304 PNKGSIEGYNGLRYDYGKYYASKTFFDSQKKRRVLWGWVNESSSVDDDIKKGWSGVQAIP 363
           P+ G ++  +  R DYGKYYASKTF+D  KKRR+LWGWVNESS   DDI+KGWSG+Q+ P
Sbjct: 275 PDLGFVQNESAPRLDYGKYYASKTFYDDVKKRRILWGWVNESSPAKDDIEKGWSGLQSFP 334

Query: 364 RAIWLDASGKQLIQWPIVEIQKLRNNKVKLTNEVLKKGSTIEVKGVTAAQADVEISFKVE 423
           R IWLD SGK+L+QWPI EI+ LR  +V    +VLK GST++V GVTAAQADVE+SFKV+
Sbjct: 335 RKIWLDESGKELLQWPIEEIETLRGQQVNWQKKVLKAGSTLQVHGVTAAQADVEVSFKVK 394

Query: 424 NLKKAEILKPEWKDPQLLCSQKGTSSTTEGGSVGPFGLLVLASNDLKEYTSVFFTVFK-- 483
            L+KA++++P W DPQ +CSQ G  S   G  +GPFGL+VLASND++EYTSV+F +FK  
Sbjct: 395 ELEKADVIEPSWTDPQKICSQ-GDLSVMSG--LGPFGLMVLASNDMEEYTSVYFRIFKSN 454

Query: 484 ----KQHKYVVLMCGDQSRSSLHSNNDKTTYGAFLDVDPTRENLSLRSLIDHSIVESFGI 543
               K+ KYVVLMC DQSRSSL+  NDK+T+GAF+ +DP+ + +SLR+LIDHSIVES+G 
Sbjct: 455 DDTNKKTKYVVLMCSDQSRSSLNDENDKSTFGAFVAIDPSHQTISLRTLIDHSIVESYGG 514

Query: 544 KGKGCITARVYPTLAIGDDVSLYAFNNGTDSVTITKLTAWSMKKAHIN 583
            G+ CIT+RVYP LAIG++ +L+ FN GT SV I  L+AWS+K A IN
Sbjct: 515 GGRTCITSRVYPKLAIGENANLFVFNKGTQSVDILTLSAWSLKSAQIN 559