CSPI07G22530 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI07G22530
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	RING-type E3 ubiquitin transferase
Location	Chr7: 19101748 .. 19105986 (+)
RNA-Seq Expression	CSPI07G22530
Synteny	CSPI07G22530

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CAACCACGGGCTGATTGCCCAAGGAAAAGTTCTACCAATTTCCCTATTTCTGCTCTGCTCTTCCATTTTGGTTGCCCATAACTGCGACAGCGGGCGTGTTTTCTTCACTGCCTCGGCAGCGGTGCTGGTCGTCGTCTCCACCGCATTCAAGGAGGGAGCTATTTTCACCACAGAAACACGGAATTTCCTCATGTCCACATCGGAGCAAGCCCTTGTTACTTTACTCTCTCGTTTTGCTTTATCGTTTGATGGAGCCGTTTTAGGCGCTGCCCTAGCTTATGCTACCGTCCGTACCGTCCTTAAACTTACTGCTTCTTGCTCCGCTCTCAATGAGCTCCGTAAAGCTCCTTATGTTACTGTTTCCGATCTTCGTCATATCGTTTCCGACGGAGTTTCGGAACAATCCGAGTCTGATCGGAAGCTTGTTGTTGTTCGTGGCACCGTGGAGACTAGGTCGTCTGTGGAAGGTAATAGGAAGAGCTTACAGCCTAATGTTTTGATTTCTCAGGAGTCTGGAGATAGAGCGGTGATTATTCAGCGAACTCAAGTGGTAACTCCTTTGAGCGACTGTGTTGAGATTTTGTTTTGTTATTTCGGGGTTAGAAAATTGCATGATTGAAAATTATTATTCATCCGTTTGTTAGTAGGTTGTGCTTATAGAACGTTGTGTGGCAGTATTCGAGTAATTTGATGGCTCCACTGAAATTGTCGACAGAGTCTAACCAATTAATCGACTAACTTGTACAGATTTAGAATTTTCACATTACAAGGTTTCTAGTCTAGGTACTCGGTTCAAGACGTAGCTACTATACAATTCTATCCTGGATGAACCTCGAAAGAGAAGAGTCATCGAGTGAAGTAGCTGTTTAATTTATCTTTATAGGTCTTCTGGCTTGCCTGAAGTTGATCTGTCATCATCGTGTGTGCCTGTGTGTATATAATGTGGGGATTAAGTATTTGGGAAATCTTGAAACTACTTAATCAACACGACTTCTTTATGTTCTGCAAGTGTTTGTTTTCGTTCCCCTTGGTTGTTTAATGTGATGGACATGTGCATATAATAATTCTGTTCTTTAAACTAAAGCTTTTTTGGTTGTATTGTGGCTTTGTTTAGTGCATATTCAACGAATGGAAAGGCTTTTTCGGATGGACTTCTGACTTGCGTGCAATTTTTGGAAGAACCTTTAGAAAGCAAGAGTCAACATCATTTAGAACGGTAAATTCAAGGCTTATATCTGTTATTGTTATGTTTGATTTGCTCTTTTCATTACCCTTACCAAAAGGGAAAAAAAAGAAGATTTTTTAAATACCTTTTTATTGAATGTTACGGGGCTTTTTGATTCTTTTCGTAAATAAAGAACTAACTAATTGAAGTGAGTGATTTTATGAGGGCATCTCTCTCAAATTTGGGGCCATAGAGTTGTGCCCTTTTCCGGTGATTATCTATTGGGTCATTTTTTCTATCTCGTTGATGGCATGTGCAAGTAGGTTTGAAAGTCATACCTCTTAAGAAATGGATCCAACAGAGTGGCTTGAAGACGTGCCCCTTAGTGTCAAGAAATAAATATTATGGTAGATTACTTATTGTCTACAAAAACTAAATCTTCATGGTATGACATTAATGTGTGGTCGTTGTGATTTGTGAAGTAATATATACACTCTAGTCTTTAGATAGGGAACAATATTCAGTGCAGTTTGCGCATGAAGAGTTATGTGATACTGATATTTGGGAAATTTTGAACAGGTTCCTTTTGTTCTTCTTGAGGGTCATTCAACATACTCTGATTTTGTTGTTGTTAACATGGAAGGCTCAAGACATCCCTTACCTCTTACAACTGTGTATCATCAACTGCAGCCCGTTTGTGCTACTCCTTATACCTTCCTTCAAGCAGTTTTTGGTCACGAGTATCCTGTGAGTTAGGCAATCTATATTGAAGTCGAGCATCACTTTTTTTTTTCTTTTTTATAAATAGATTTTAGGTAAAATTCTTAGTTATGCTCTACTTTTGAAGGTTGGGGTGCTTGATGAGGAGAAAATACTTCCATTAGGAAAAAATATCAGTGCTGTTGGTATATGCAGTTTTGAAAATGGAGTTCCTGTCATAAAGTCTTGCAGTGATTTTCCCCATTTCCTGTAAGTTTATACATTTCAAAGTAGATCATAGATGCACATCATATCTTATTTCTCTCAACTTGTGGACATATAATTGATGATAAATTTGTTCTGTCGGCCAATGGTGAGCCCTGTTGATTGGGTTTGGGGCTCTGGATGGGACTTCAACTAGGTCTTAGGATTAAGCCTCCGAAAGCTTTTGTTGTCTTTTGGAGCTGCATGGGTTTGTTTGTATTTGTTGTCTGGGTCCCAATACTCTCTTCTTCATACAAATGTGTGTGTGTGTTGTGCACGTTCTCATTTCTTGTTCAAGTGTGGGCCTTGGTTTCCTAAATCATGCAACAGATCTTGTTTCGTTAGCACTGCTAGCTAGTGGTTAGTCTCTCCTCTCCCGCATACCCAAAGTGAGAAAAAAAAAACTGTAGGCAACTAATTGAAGCCATGTCTGATGTGTGACATTCATGCAATTTCCTCTTTGTTGTATATGGAGAATATTGTCTAATTGGACAACAAAAAATCGTTTCCTTTATGTTTTCCTTGTATTTGGTATTTTGAGTACTAGCCTTTTTTCATCTATTCATTCAAAAGAAAGAACTGTCTAATTGGACAAAAATTGTTCCTGATTACGCTTTCAAGACAATCTTCATTTAAATATTAATAACAAATATTATGATGATGTATTTTTCTCTGCAGATGTGAAATGACTAAGGATCAGATGATTTTAGATCTAGTCTTCAAAACAAAATTTCTATTTTGGAGTAGTATTGTCCTTGGTTCATTGACAGTTGGCATTCTTGGTTATTCTGCTGCGAGGTATGTTTTATTTTGTTAATTTTTTCTTTTATTTTATTTGGTTGAGGTATCTGTAAGGACACATCCTCATGATTTGATATTCACATGATAATAACAAAAGTAAAATAGAGTCCGACTAACACAAGAATTACGAACAAAACAAACAAACCCCAACCAAGCGAGAGGGTTGCACTTTTCCACGGTTGGACTATTCTTTACACACTAAACACAATATAACAAACCCTAGCCAAAATGATAGTTTATATACAAACATTCCCCTGCAACACGTACATCCAGGACTACAACCATCGAACCCTCCATCTGGGGTGTAATCCCCTTTCTACTCTTCCTCTTATATACTTTCTTAATGGAAGGCCTATCTGTACCTTTGTGCTGATTCTTGATATTTTTCTTTAGGCAAAGCAATACCTTGGCTTAATATACCTTCCATGAATTCTTTTCTTTTTTTCTGCTTTGCACTATACTTTTTGAATATTTTTCATTAGTCCTTTTATTAGACTATTCCGAAGTATAATACCCCACTCGTCTTATTGCTTCCTTCTTTCTATAATCACATTTCGAAAAGTTTAAGGAGTGCCAATATCCATTCTCTTCAGAAATTGGAATAGATGGAAACAATGGAGGCAACACAGACAGTTACAGAATTCAAGGAATGATTCCCCCACGTCACAACTGGATTCAAGCAATGACATTCCTATTTACGGTGATTTTGCTTCAGACGACGAGGCAGCAACTGTTCCAGACGATGAGTTATCATCACACGTTCCAGACGATGAGTTATCATCACACGTTCCAGATGGACAATTGTGTGTGATCTGTCTCATGAGGAGAAAACGATCTGCTTTCATTCCATGCGGTCATCTAGTTTGTTGCGAACGATGTGCTGTGTCAGTCGAACGTGAGTCATCTCCCAAATGTCCCATTTGCCGCCAACAAATTCGTAGTTCTGTACGGATTTACGATTCGTGAAGAGACAAATCATCTTTTCAGGTTCTAACTACGACAAATCCCGTTTTGCTGATATTATACTGTTTGAGATTTCATATAAATGCCAATCCCTTCCACAAGATAGCAGATGTGAAGTTTGTTTTTCCTCATCTACTTGTAACTTTTTATCCCCACATTTTGATGAGAGGGATTGTTCTTTAAAGTTTGTAAAGCCAGATTTGAAGTAATGGCTCTTGTAAATATCAGATAGCATATACTCTTTTGATATGTGACATGAAAAAAATGAAACTTTATTATTGGCTCCGTTATGTATAGGTGTATTGCTCGGATTGACTTTCCAAATGGTTTAGAAAAATAAATATGAT

mRNA sequence

CAACCACGGGCTGATTGCCCAAGGAAAAGTTCTACCAATTTCCCTATTTCTGCTCTGCTCTTCCATTTTGGTTGCCCATAACTGCGACAGCGGGCGTGTTTTCTTCACTGCCTCGGCAGCGGTGCTGGTCGTCGTCTCCACCGCATTCAAGGAGGGAGCTATTTTCACCACAGAAACACGGAATTTCCTCATGTCCACATCGGAGCAAGCCCTTGTTACTTTACTCTCTCGTTTTGCTTTATCGTTTGATGGAGCCGTTTTAGGCGCTGCCCTAGCTTATGCTACCGTCCGTACCGTCCTTAAACTTACTGCTTCTTGCTCCGCTCTCAATGAGCTCCGTAAAGCTCCTTATGTTACTGTTTCCGATCTTCGTCATATCGTTTCCGACGGAGTTTCGGAACAATCCGAGTCTGATCGGAAGCTTGTTGTTGTTCGTGGCACCGTGGAGACTAGGTCGTCTGTGGAAGGTAATAGGAAGAGCTTACAGCCTAATGTTTTGATTTCTCAGGAGTCTGGAGATAGAGCGGTGATTATTCAGCGAACTCAAGTGTGCATATTCAACGAATGGAAAGGCTTTTTCGGATGGACTTCTGACTTGCGTGCAATTTTTGGAAGAACCTTTAGAAAGCAAGAGTCAACATCATTTAGAACGGTTCCTTTTGTTCTTCTTGAGGGTCATTCAACATACTCTGATTTTGTTGTTGTTAACATGGAAGGCTCAAGACATCCCTTACCTCTTACAACTGTGTATCATCAACTGCAGCCCGTTTGTGCTACTCCTTATACCTTCCTTCAAGCAGTTTTTGGTCACGAGTATCCTGTTGGGGTGCTTGATGAGGAGAAAATACTTCCATTAGGAAAAAATATCAGTGCTGTTGGTATATGCAGTTTTGAAAATGGAGTTCCTGTCATAAAGTCTTGCAGTGATTTTCCCCATTTCCTATGTGAAATGACTAAGGATCAGATGATTTTAGATCTAGTCTTCAAAACAAAATTTCTATTTTGGAGTAGTATTGTCCTTGGTTCATTGACAGTTGGCATTCTTGGTTATTCTGCTGCGAGAAATTGGAATAGATGGAAACAATGGAGGCAACACAGACAGTTACAGAATTCAAGGAATGATTCCCCCACGTCACAACTGGATTCAAGCAATGACATTCCTATTTACGGTGATTTTGCTTCAGACGACGAGGCAGCAACTGTTCCAGACGATGAGTTATCATCACACGTTCCAGACGATGAGTTATCATCACACGTTCCAGATGGACAATTGTGTGTGATCTGTCTCATGAGGAGAAAACGATCTGCTTTCATTCCATGCGGTCATCTAGTTTGTTGCGAACGATGTGCTGTGTCAGTCGAACGTGAGTCATCTCCCAAATGTCCCATTTGCCGCCAACAAATTCGTAGTTCTGTACGGATTTACGATTCGTGAAGAGACAAATCATCTTTTCAGGTTCTAACTACGACAAATCCCGTTTTGCTGATATTATACTGTTTGAGATTTCATATAAATGCCAATCCCTTCCACAAGATAGCAGATGTGAAGTTTGTTTTTCCTCATCTACTTGTAACTTTTTATCCCCACATTTTGATGAGAGGGATTGTTCTTTAAAGTTTGTAAAGCCAGATTTGAAGTAATGGCTCTTGTAAATATCAGATAGCATATACTCTTTTGATATGTGACATGAAAAAAATGAAACTTTATTATTGGCTCCGTTATGTATAGGTGTATTGCTCGGATTGACTTTCCAAATGGTTTAGAAAAATAAATATGAT

Coding sequence (CDS)

ATGTCCACATCGGAGCAAGCCCTTGTTACTTTACTCTCTCGTTTTGCTTTATCGTTTGATGGAGCCGTTTTAGGCGCTGCCCTAGCTTATGCTACCGTCCGTACCGTCCTTAAACTTACTGCTTCTTGCTCCGCTCTCAATGAGCTCCGTAAAGCTCCTTATGTTACTGTTTCCGATCTTCGTCATATCGTTTCCGACGGAGTTTCGGAACAATCCGAGTCTGATCGGAAGCTTGTTGTTGTTCGTGGCACCGTGGAGACTAGGTCGTCTGTGGAAGGTAATAGGAAGAGCTTACAGCCTAATGTTTTGATTTCTCAGGAGTCTGGAGATAGAGCGGTGATTATTCAGCGAACTCAAGTGTGCATATTCAACGAATGGAAAGGCTTTTTCGGATGGACTTCTGACTTGCGTGCAATTTTTGGAAGAACCTTTAGAAAGCAAGAGTCAACATCATTTAGAACGGTTCCTTTTGTTCTTCTTGAGGGTCATTCAACATACTCTGATTTTGTTGTTGTTAACATGGAAGGCTCAAGACATCCCTTACCTCTTACAACTGTGTATCATCAACTGCAGCCCGTTTGTGCTACTCCTTATACCTTCCTTCAAGCAGTTTTTGGTCACGAGTATCCTGTTGGGGTGCTTGATGAGGAGAAAATACTTCCATTAGGAAAAAATATCAGTGCTGTTGGTATATGCAGTTTTGAAAATGGAGTTCCTGTCATAAAGTCTTGCAGTGATTTTCCCCATTTCCTATGTGAAATGACTAAGGATCAGATGATTTTAGATCTAGTCTTCAAAACAAAATTTCTATTTTGGAGTAGTATTGTCCTTGGTTCATTGACAGTTGGCATTCTTGGTTATTCTGCTGCGAGAAATTGGAATAGATGGAAACAATGGAGGCAACACAGACAGTTACAGAATTCAAGGAATGATTCCCCCACGTCACAACTGGATTCAAGCAATGACATTCCTATTTACGGTGATTTTGCTTCAGACGACGAGGCAGCAACTGTTCCAGACGATGAGTTATCATCACACGTTCCAGACGATGAGTTATCATCACACGTTCCAGATGGACAATTGTGTGTGATCTGTCTCATGAGGAGAAAACGATCTGCTTTCATTCCATGCGGTCATCTAGTTTGTTGCGAACGATGTGCTGTGTCAGTCGAACGTGAGTCATCTCCCAAATGTCCCATTTGCCGCCAACAAATTCGTAGTTCTGTACGGATTTACGATTCGTGA

Protein sequence

MSTSEQALVTLLSRFALSFDGAVLGAALAYATVRTVLKLTASCSALNELRKAPYVTVSDLRHIVSDGVSEQSESDRKLVVVRGTVETRSSVEGNRKSLQPNVLISQESGDRAVIIQRTQVCIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLLEGHSTYSDFVVVNMEGSRHPLPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKNISAVGICSFENGVPVIKSCSDFPHFLCEMTKDQMILDLVFKTKFLFWSSIVLGSLTVGILGYSAARNWNRWKQWRQHRQLQNSRNDSPTSQLDSSNDIPIYGDFASDDEAATVPDDELSSHVPDDELSSHVPDGQLCVICLMRRKRSAFIPCGHLVCCERCAVSVERESSPKCPICRQQIRSSVRIYDS*

Homology

[Close]

BLAST of CSPI07G22530 vs. ExPASy Swiss-Prot
Match: Q9SYH3 (E3 ubiquitin-protein ligase SPL2 OS=Arabidopsis thaliana OX=3702 GN=SPL2 PE=2 SV=1)

HSP 1 Score: 362.5 bits (929), Expect = 6.6e-99
Identity = 204/421 (48.46%), Postives = 279/421 (66.27%), Query Frame = 0


Query: 1   MSTSEQALVTLLSRFALSFDGAVLGAALAYATVRTVLKLTASCSALNELRKAPYVTVSDL 60
           MS+ E+AL+ LL+  ALSFDGA+LG  LA + V + LK  ++ +AL +++ AP V++SDL
Sbjct: 1   MSSPERALLNLLTDIALSFDGAILGLTLAVSAVGSALKYASTNAALKKIKDAPEVSISDL 60

Query: 61  RHIV--SDGVSEQSE----SDRKLVVVRGTVETRSSVEGNRKSLQPNVLISQESGDRAVI 120
           R ++  S+  SE ++    +D+++VVVRG V+ +  + G+      NVLIS E+GD+A+I
Sbjct: 61  RSLLPASEDKSETNDNRKSNDQRIVVVRGVVKPK--ISGDEGYKNNNVLISPETGDKALI 120

Query: 121 IQRTQVCIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLL-EGHSTYSDFVVVN 180
           IQRTQ  +++ WK  F  T   R +  R+ RK  +   RTVPFV++ +   + S FV VN
Sbjct: 121 IQRTQTYVYSGWKRLFQSTGH-RFMLERSLRKHGADFTRTVPFVIVGKDQQSNSSFVAVN 180

Query: 181 MEGSRHPLPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKNISAVGICS 240
           M+GSR PLPLTTVY++LQP+ +   +FLQA    +YPVG+LD EKILP GK+I+AVGI S
Sbjct: 181 MDGSRQPLPLTTVYNRLQPINS---SFLQAFLYPDYPVGLLDIEKILPPGKDITAVGIYS 240

Query: 241 FENGVPVIKSCSDFPHFLCEMTKDQMILDLVFKTKFLFWSSIVLGSLTVGILGYSAARNW 300
           F NGVP IKSC D P+FL EMTKD+MI DL+ +T F+F  S++LG ++VGIL Y+A R W
Sbjct: 241 FNNGVPEIKSCQDLPYFLSEMTKDKMIEDLMEQTNFIFLGSVILGIVSVGILSYAAVRTW 300

Query: 301 NRWKQWRQHRQLQNSRNDSPTSQLDSSNDIPIYGDFASDDEAATVPDDELSSHVPDDELS 360
           N+WKQW   R+L    NDS                         V DDE     P+D  +
Sbjct: 301 NKWKQWNHQRELPQRPNDS-------------------------VVDDE-----PED--A 360

Query: 361 SHVPDGQLCVICLMRRKRSAFIPCGHLVCCERCAVSVERESSPKCPICRQQIRSSVRIYD 415
             +PDG+LCVIC+ RR+  AFIPCGH+VCC RCA +VERE +PKCP+C Q IR S+R+Y 
Sbjct: 361 DEIPDGELCVICVSRRRVPAFIPCGHVVCCRRCASTVERELNPKCPVCLQSIRGSMRVYY 383

[Close]

BLAST of CSPI07G22530 vs. ExPASy Swiss-Prot
Match: P41437 (E3 ubiquitin-protein ligase IAP-3 OS=Orgyia pseudotsugata multicapsid polyhedrosis virus OX=262177 GN=IAP3 PE=3 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 2.2e-09
Identity = 31/79 (39.24%), Postives = 43/79 (54.43%), Query Frame = 0


Query: 334 EAATVPDDELSSHVPDDELSSHVPDGQLCVICLMRRKRSAFIPCGHLVCCERCAVSVERE 393
           EA  V D +   H+    + + V D +LC ICL   K   F+PCGH+V C +CA  V   
Sbjct: 193 EACVVRDADNEPHIERPAVEAEVADDRLCKICLGAEKTVCFVPCGHVVACGKCAAGV--- 252

Query: 394 SSPKCPICRQQIRSSVRIY 413
               CP+CR Q+  +VR+Y
Sbjct: 253 --TTCPVCRGQLDKAVRMY 266

[Close]

BLAST of CSPI07G22530 vs. ExPASy Swiss-Prot
Match: O08863 (Baculoviral IAP repeat-containing protein 3 OS=Mus musculus OX=10090 GN=Birc3 PE=1 SV=2)

HSP 1 Score: 60.5 bits (145), Expect = 5.3e-08
Identity = 35/105 (33.33%), Postives = 59/105 (56.19%), Query Frame = 0


Query: 310 NDSPTSQLDSSNDI--PIYGDFASDDEAATVPDDELSSHVPDDELSSHVPDGQLCVICLM 369
           N + TS  +S  +I   +Y D     +  ++P D++++ +P +E    + + ++C +C+ 
Sbjct: 500 NTAATSFRNSLREIDPALYRDIFVQQDIRSLPTDDIAA-LPMEEQLRKLQEERMCKVCMD 559

Query: 370 RRKRSAFIPCGHLVCCERCAVSVERESSPKCPICRQQIRSSVRIY 413
           R     FIPCGHLV C+ CA S+      KCPICR  I+ +VR +
Sbjct: 560 REVSIVFIPCGHLVVCKDCAPSLR-----KCPICRGTIKGTVRTF 598

[Close]

BLAST of CSPI07G22530 vs. ExPASy Swiss-Prot
Match: P41436 (Apoptosis inhibitor IAP OS=Cydia pomonella granulosis virus (isolate Mexico/1963) OX=654905 GN=IAP PE=4 SV=1)

HSP 1 Score: 59.7 bits (143), Expect = 9.1e-08
Identity = 27/74 (36.49%), Postives = 45/74 (60.81%), Query Frame = 0


Query: 343 LSSHVPDDELSS--HVPDGQLCVICLMRRKRSAFIPCGHLVCCERCAVSVERESSPKCPI 402
           +S  +P+ ++     V D +LC IC +      F+PCGH+V C +CA+SV+     KCP+
Sbjct: 207 VSEPIPETKIEKEPQVEDSKLCKICYVEECIVCFVPCGHVVACAKCALSVD-----KCPM 266

Query: 403 CRQQIRSSVRIYDS 415
           CR+ + S +++Y S
Sbjct: 267 CRKIVTSVLKVYFS 275

[Close]

BLAST of CSPI07G22530 vs. ExPASy Swiss-Prot
Match: Q24306 (Death-associated inhibitor of apoptosis 1 OS=Drosophila melanogaster OX=7227 GN=Diap1 PE=1 SV=2)

HSP 1 Score: 59.3 bits (142), Expect = 1.2e-07
Identity = 26/62 (41.94%), Postives = 38/62 (61.29%), Query Frame = 0


Query: 353 SSHVPDGQLCVICLMRRKRSAFIPCGHLVCCERCAVSVERESSPKCPICRQQIRSSVRIY 412
           S+ +P+ +LC IC      +AF+PCGH+V C +CA SV      KCP+CR+     +R+Y
Sbjct: 382 STSIPEEKLCKICYGAEYNTAFLPCGHVVACAKCASSV-----TKCPLCRKPFTDVMRVY 438

Query: 413 DS 415
            S
Sbjct: 442 FS 438

[Close]

BLAST of CSPI07G22530 vs. ExPASy TrEMBL
Match: A0A0A0K708 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_7G450490 PE=4 SV=1)

HSP 1 Score: 801.2 bits (2068), Expect = 2.0e-228
Identity = 405/414 (97.83%), Postives = 405/414 (97.83%), Query Frame = 0


Query: 1   MSTSEQALVTLLSRFALSFDGAVLGAALAYATVRTVLKLTASCSALNELRKAPYVTVSDL 60
           MSTSEQALVTLLSRFALSFDGAVLGAALAYATVRTVLKLTASCSALNELRKAPYVTVSDL
Sbjct: 1   MSTSEQALVTLLSRFALSFDGAVLGAALAYATVRTVLKLTASCSALNELRKAPYVTVSDL 60

Query: 61  RHIVSDGVSEQSESDRKLVVVRGTVETRSSVEGNRKSLQPNVLISQESGDRAVIIQRTQV 120
           RHIVSDGVSEQSESDRKLVVVRGTVETRSSVEGNRKSLQPNVLISQESGDRAVIIQRTQV
Sbjct: 61  RHIVSDGVSEQSESDRKLVVVRGTVETRSSVEGNRKSLQPNVLISQESGDRAVIIQRTQV 120

Query: 121 CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLLEGHSTYSDFVVVNMEGSRHP 180
           CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLLEGHSTYSDFVVVNMEGSRHP
Sbjct: 121 CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLLEGHSTYSDFVVVNMEGSRHP 180

Query: 181 LPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKNISAVGICSFENGVPV 240
           LPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKNISAVGICSFENGVPV
Sbjct: 181 LPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKNISAVGICSFENGVPV 240

Query: 241 IKSCSDFPHFLCEMTKDQMILDLVFKTKFLFWSSIVLGSLTVGILGYSAARNWNRWKQWR 300
           IKSCSDFPHFLCEMTKDQMILDLVFKTKFLFWSSIVLGSLTVGILGYSAARNWNRWKQWR
Sbjct: 241 IKSCSDFPHFLCEMTKDQMILDLVFKTKFLFWSSIVLGSLTVGILGYSAARNWNRWKQWR 300

Query: 301 QHRQLQNSRNDSPTSQLDSSNDIPIYGDFASDDEAATVPDDELSSHVPDDELSSHVPDGQ 360
           QHRQLQNSRNDSPTSQLDSSNDIPIYGDFASDDEAAT         VPDDELSSHVPDGQ
Sbjct: 301 QHRQLQNSRNDSPTSQLDSSNDIPIYGDFASDDEAAT---------VPDDELSSHVPDGQ 360

Query: 361 LCVICLMRRKRSAFIPCGHLVCCERCAVSVERESSPKCPICRQQIRSSVRIYDS 415
           LCVICLMRRKRSAFIPCGHLVCCERCAVSVERESSPKCPICRQQIRSSVRIYDS
Sbjct: 361 LCVICLMRRKRSAFIPCGHLVCCERCAVSVERESSPKCPICRQQIRSSVRIYDS 405

[Close]

BLAST of CSPI07G22530 vs. ExPASy TrEMBL
Match: A0A5D3BWQ0 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G001240 PE=4 SV=1)

HSP 1 Score: 783.1 bits (2021), Expect = 5.7e-223
Identity = 396/414 (95.65%), Postives = 401/414 (96.86%), Query Frame = 0


Query: 1   MSTSEQALVTLLSRFALSFDGAVLGAALAYATVRTVLKLTASCSALNELRKAPYVTVSDL 60
           MSTSEQALVTLLSRFALSFDGAVLGAALAYATVRTVLKLTASCSALNELRKAPYVTVSDL
Sbjct: 1   MSTSEQALVTLLSRFALSFDGAVLGAALAYATVRTVLKLTASCSALNELRKAPYVTVSDL 60

Query: 61  RHIVSDGVSEQSESDRKLVVVRGTVETRSSVEGNRKSLQPNVLISQESGDRAVIIQRTQV 120
           RHIVSDG SEQSESDRKLVVVRGTVETRSSVEGN KSLQPNVLISQESGDRAVIIQRTQV
Sbjct: 61  RHIVSDGNSEQSESDRKLVVVRGTVETRSSVEGNWKSLQPNVLISQESGDRAVIIQRTQV 120

Query: 121 CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLLEGHSTYSDFVVVNMEGSRHP 180
           CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVL++GHSTYSDFVVVNMEGSRHP
Sbjct: 121 CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLVDGHSTYSDFVVVNMEGSRHP 180

Query: 181 LPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKNISAVGICSFENGVPV 240
           LPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKNISAVGICSFENGVPV
Sbjct: 181 LPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKNISAVGICSFENGVPV 240

Query: 241 IKSCSDFPHFLCEMTKDQMILDLVFKTKFLFWSSIVLGSLTVGILGYSAARNWNRWKQWR 300
           IKSCSDFPHFLCEMTKDQMILDLVFKTKFLFWSSIVLGSLTVGILGYSA RNWNRWKQWR
Sbjct: 241 IKSCSDFPHFLCEMTKDQMILDLVFKTKFLFWSSIVLGSLTVGILGYSAVRNWNRWKQWR 300

Query: 301 QHRQLQNSRNDSPTSQLDSSNDIPIYGDFASDDEAATVPDDELSSHVPDDELSSHVPDGQ 360
           QHR+LQNSRNDS TSQLDSSNDIPIYGDFASDDEAAT         VPDDELSS+VPDGQ
Sbjct: 301 QHRRLQNSRNDSTTSQLDSSNDIPIYGDFASDDEAAT---------VPDDELSSNVPDGQ 360

Query: 361 LCVICLMRRKRSAFIPCGHLVCCERCAVSVERESSPKCPICRQQIRSSVRIYDS 415
           LCVICLMRRKRSAFIPCGHLVCCERCAVSVER+SSPKCPICRQQIRSSVRIYDS
Sbjct: 361 LCVICLMRRKRSAFIPCGHLVCCERCAVSVERDSSPKCPICRQQIRSSVRIYDS 405

[Close]

BLAST of CSPI07G22530 vs. ExPASy TrEMBL
Match: A0A1S3CGP0 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103500694 PE=4 SV=1)

HSP 1 Score: 783.1 bits (2021), Expect = 5.7e-223
Identity = 396/414 (95.65%), Postives = 401/414 (96.86%), Query Frame = 0


Query: 1   MSTSEQALVTLLSRFALSFDGAVLGAALAYATVRTVLKLTASCSALNELRKAPYVTVSDL 60
           MSTSEQALVTLLSRFALSFDGAVLGAALAYATVRTVLKLTASCSALNELRKAPYVTVSDL
Sbjct: 1   MSTSEQALVTLLSRFALSFDGAVLGAALAYATVRTVLKLTASCSALNELRKAPYVTVSDL 60

Query: 61  RHIVSDGVSEQSESDRKLVVVRGTVETRSSVEGNRKSLQPNVLISQESGDRAVIIQRTQV 120
           RHIVSDG SEQSESDRKLVVVRGTVETRSSVEGN KSLQPNVLISQESGDRAVIIQRTQV
Sbjct: 61  RHIVSDGNSEQSESDRKLVVVRGTVETRSSVEGNWKSLQPNVLISQESGDRAVIIQRTQV 120

Query: 121 CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLLEGHSTYSDFVVVNMEGSRHP 180
           CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVL++GHSTYSDFVVVNMEGSRHP
Sbjct: 121 CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLVDGHSTYSDFVVVNMEGSRHP 180

Query: 181 LPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKNISAVGICSFENGVPV 240
           LPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKNISAVGICSFENGVPV
Sbjct: 181 LPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKNISAVGICSFENGVPV 240

Query: 241 IKSCSDFPHFLCEMTKDQMILDLVFKTKFLFWSSIVLGSLTVGILGYSAARNWNRWKQWR 300
           IKSCSDFPHFLCEMTKDQMILDLVFKTKFLFWSSIVLGSLTVGILGYSA RNWNRWKQWR
Sbjct: 241 IKSCSDFPHFLCEMTKDQMILDLVFKTKFLFWSSIVLGSLTVGILGYSAVRNWNRWKQWR 300

Query: 301 QHRQLQNSRNDSPTSQLDSSNDIPIYGDFASDDEAATVPDDELSSHVPDDELSSHVPDGQ 360
           QHR+LQNSRNDS TSQLDSSNDIPIYGDFASDDEAAT         VPDDELSS+VPDGQ
Sbjct: 301 QHRRLQNSRNDSTTSQLDSSNDIPIYGDFASDDEAAT---------VPDDELSSNVPDGQ 360

Query: 361 LCVICLMRRKRSAFIPCGHLVCCERCAVSVERESSPKCPICRQQIRSSVRIYDS 415
           LCVICLMRRKRSAFIPCGHLVCCERCAVSVER+SSPKCPICRQQIRSSVRIYDS
Sbjct: 361 LCVICLMRRKRSAFIPCGHLVCCERCAVSVERDSSPKCPICRQQIRSSVRIYDS 405

[Close]

BLAST of CSPI07G22530 vs. ExPASy TrEMBL
Match: A0A6J1JT39 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111488642 PE=4 SV=1)

HSP 1 Score: 704.9 bits (1818), Expect = 2.0e-199
Identity = 358/417 (85.85%), Postives = 385/417 (92.33%), Query Frame = 0


Query: 1   MSTSEQALVTLLSRFALSFDGAVLGAALAYATVRTVLKLTASCSALNELRKAPYVTVSDL 60
           MS+SEQALV+LLS+FALSFDGAVLGAALAYA VRTVLKLTASCSAL+ELRKAPYV+VSDL
Sbjct: 1   MSSSEQALVSLLSQFALSFDGAVLGAALAYAAVRTVLKLTASCSALDELRKAPYVSVSDL 60

Query: 61  RHIVSDGVSEQSESDRKLVVVRGTVETRSSVEGNRKSLQPNVLISQESGDRAVIIQRTQV 120
           RHI+SDG S +SESD KLVVVRGTVE RSSVEGN KS+QPNVLISQESGDRAVIIQRTQ+
Sbjct: 61  RHIISDGDSGKSESDHKLVVVRGTVEARSSVEGNWKSVQPNVLISQESGDRAVIIQRTQM 120

Query: 121 CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLL-EGHSTYSDFVVVNMEGSRH 180
           CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVL+ EGHS YSDFVVVNM+GSRH
Sbjct: 121 CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLVDEGHSPYSDFVVVNMDGSRH 180

Query: 181 PLPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKNISAVGICSFENGVP 240
           PLPLTTVYHQLQPV ATPYTFLQA+FGHEYPVGVLDEEKILPLGKNISAVGICSF+NGVP
Sbjct: 181 PLPLTTVYHQLQPVSATPYTFLQALFGHEYPVGVLDEEKILPLGKNISAVGICSFKNGVP 240

Query: 241 VIKSCSDFPHFLCEMTKDQMILDLVFKTKFLFWSSIVLGSLTVGILGYSAARNWNRWKQW 300
           VIKSC DFPHFLCEMTKDQMILDLVFKTKFLFWSS+VLGSL+VG+LGYS  RNWNRWKQW
Sbjct: 241 VIKSCKDFPHFLCEMTKDQMILDLVFKTKFLFWSSVVLGSLSVGVLGYSVVRNWNRWKQW 300

Query: 301 RQHRQLQNSRNDSPTSQLDSSNDIPIYGDF--ASDDEAATVPDDELSSHVPDDELSSHVP 360
           RQ R+LQ+S NDS  SQLD SN+IPIYGDF  ASD+E A           P+DE+SS+VP
Sbjct: 301 RQQRRLQDSGNDSTMSQLD-SNNIPIYGDFGTASDNEEAV---------DPEDEVSSNVP 360

Query: 361 DGQLCVICLMRRKRSAFIPCGHLVCCERCAVSVERESSPKCPICRQQIRSSVRIYDS 415
           DGQLCVICLMRRKRSAFIPCGH VCCERCA+SVER+S+PKCP+CRQQIRSSVRIYDS
Sbjct: 361 DGQLCVICLMRRKRSAFIPCGHFVCCERCAMSVERDSAPKCPVCRQQIRSSVRIYDS 407

[Close]

BLAST of CSPI07G22530 vs. ExPASy TrEMBL
Match: A0A6J1HK84 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111464304 PE=4 SV=1)

HSP 1 Score: 702.2 bits (1811), Expect = 1.3e-198
Identity = 350/414 (84.54%), Postives = 378/414 (91.30%), Query Frame = 0


Query: 1   MSTSEQALVTLLSRFALSFDGAVLGAALAYATVRTVLKLTASCSALNELRKAPYVTVSDL 60
           MS+SEQALV+L SRFALSFDGA LGAALAYA  RTVLKLTASCSAL+ELRKAP V VSDL
Sbjct: 1   MSSSEQALVSLFSRFALSFDGAALGAALAYAAFRTVLKLTASCSALDELRKAPNVRVSDL 60

Query: 61  RHIVSDGVSEQSESDRKLVVVRGTVETRSSVEGNRKSLQPNVLISQESGDRAVIIQRTQV 120
           RH++SDG S QSESD+KLVVVRGTVE RS VEGN KSLQPNVLISQESGDRAVIIQRTQ+
Sbjct: 61  RHMISDGDSRQSESDQKLVVVRGTVEARSFVEGNWKSLQPNVLISQESGDRAVIIQRTQM 120

Query: 121 CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLLEGHSTYSDFVVVNMEGSRHP 180
           CIFNEWKGF GWTSDLRAIFGRTFRKQESTSFRTVPFVL+EGHS YSDF VVNM+GSRH 
Sbjct: 121 CIFNEWKGFLGWTSDLRAIFGRTFRKQESTSFRTVPFVLVEGHSPYSDFAVVNMDGSRHR 180

Query: 181 LPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKNISAVGICSFENGVPV 240
           LPLTTVY QLQPVCATPYTFLQA+FGHEYPVGVLDEEKILPLG+NIS VG+CSF+NGVP 
Sbjct: 181 LPLTTVYRQLQPVCATPYTFLQALFGHEYPVGVLDEEKILPLGRNISVVGVCSFKNGVPE 240

Query: 241 IKSCSDFPHFLCEMTKDQMILDLVFKTKFLFWSSIVLGSLTVGILGYSAARNWNRWKQWR 300
           IKSC DFPHFLCEMTKDQM+LDLVFKTKFLFWSSIVLGSL+VG+LG S  RNWNRWKQWR
Sbjct: 241 IKSCKDFPHFLCEMTKDQMVLDLVFKTKFLFWSSIVLGSLSVGVLGCSVMRNWNRWKQWR 300

Query: 301 QHRQLQNSRNDSPTSQLDSSNDIPIYGDFASDDEAATVPDDELSSHVPDDELSSHVPDGQ 360
           Q R+LQ+S NDS  SQLDSSN+IPIYGDF       T  DDE++S +PDDELSS+VPDGQ
Sbjct: 301 QQRRLQDSGNDSTMSQLDSSNNIPIYGDF------GTASDDEVAS-IPDDELSSNVPDGQ 360

Query: 361 LCVICLMRRKRSAFIPCGHLVCCERCAVSVERESSPKCPICRQQIRSSVRIYDS 415
           LCVICLMRR+RSAFIPCGHLVCCERCA+SVER+S+PKCP+CRQQIRSSVRIYDS
Sbjct: 361 LCVICLMRRRRSAFIPCGHLVCCERCAISVERDSAPKCPVCRQQIRSSVRIYDS 407

[Close]

BLAST of CSPI07G22530 vs. NCBI nr
Match: XP_011659622.2 (E3 ubiquitin-protein ligase SPL2 isoform X1 [Cucumis sativus])

HSP 1 Score: 818.5 bits (2113), Expect = 2.5e-233
Identity = 414/423 (97.87%), Postives = 414/423 (97.87%), Query Frame = 0


Query: 1   MSTSEQALVTLLSRFALSFDGAVLGAALAYATVRTVLKLTASCSALNELRKAPYVTVSDL 60
           MSTSEQALVTLLSRFALSFDGAVLGAALAYATVRTVLKLTASCSALNELRKAPYVTVSDL
Sbjct: 1   MSTSEQALVTLLSRFALSFDGAVLGAALAYATVRTVLKLTASCSALNELRKAPYVTVSDL 60

Query: 61  RHIVSDGVSEQSESDRKLVVVRGTVETRSSVEGNRKSLQPNVLISQESGDRAVIIQRTQV 120
           RHIVSDGVSEQSESDRKLVVVRGTVETRSSVEGNRKSLQPNVLISQESGDRAVIIQRTQV
Sbjct: 61  RHIVSDGVSEQSESDRKLVVVRGTVETRSSVEGNRKSLQPNVLISQESGDRAVIIQRTQV 120

Query: 121 CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLLEGHSTYSDFVVVNMEGSRHP 180
           CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLLEGHSTYSDFVVVNMEGSRHP
Sbjct: 121 CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLLEGHSTYSDFVVVNMEGSRHP 180

Query: 181 LPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKNISAVGICSFENGVPV 240
           LPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKNISAVGICSFENGVPV
Sbjct: 181 LPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKNISAVGICSFENGVPV 240

Query: 241 IKSCSDFPHFLCEMTKDQMILDLVFKTKFLFWSSIVLGSLTVGILGYSAARNWNRWKQWR 300
           IKSCSDFPHFLCEMTKDQMILDLVFKTKFLFWSSIVLGSLTVGILGYSAARNWNRWKQWR
Sbjct: 241 IKSCSDFPHFLCEMTKDQMILDLVFKTKFLFWSSIVLGSLTVGILGYSAARNWNRWKQWR 300

Query: 301 QHRQLQNSRNDSPTSQLDSSNDIPIYGDFASDDEAAT---------VPDDELSSHVPDDE 360
           QHRQLQNSRNDSPTSQLDSSNDIPIYGDFASDDEAAT         VPDDELSSHVPDDE
Sbjct: 301 QHRQLQNSRNDSPTSQLDSSNDIPIYGDFASDDEAATVPDDELSSHVPDDELSSHVPDDE 360

Query: 361 LSSHVPDGQLCVICLMRRKRSAFIPCGHLVCCERCAVSVERESSPKCPICRQQIRSSVRI 415
           LSSHVPDGQLCVICLMRRKRSAFIPCGHLVCCERCAVSVERESSPKCPICRQQIRSSVRI
Sbjct: 361 LSSHVPDGQLCVICLMRRKRSAFIPCGHLVCCERCAVSVERESSPKCPICRQQIRSSVRI 420

[Close]

BLAST of CSPI07G22530 vs. NCBI nr
Match: XP_008462309.1 (PREDICTED: mitochondrial ubiquitin ligase activator of nfkb 1 [Cucumis melo] >KAA0059400.1 mitochondrial ubiquitin ligase activator of nfkb 1 [Cucumis melo var. makuwa] >TYK03927.1 mitochondrial ubiquitin ligase activator of nfkb 1 [Cucumis melo var. makuwa])

HSP 1 Score: 783.1 bits (2021), Expect = 1.2e-222
Identity = 396/414 (95.65%), Postives = 401/414 (96.86%), Query Frame = 0


Query: 1   MSTSEQALVTLLSRFALSFDGAVLGAALAYATVRTVLKLTASCSALNELRKAPYVTVSDL 60
           MSTSEQALVTLLSRFALSFDGAVLGAALAYATVRTVLKLTASCSALNELRKAPYVTVSDL
Sbjct: 1   MSTSEQALVTLLSRFALSFDGAVLGAALAYATVRTVLKLTASCSALNELRKAPYVTVSDL 60

Query: 61  RHIVSDGVSEQSESDRKLVVVRGTVETRSSVEGNRKSLQPNVLISQESGDRAVIIQRTQV 120
           RHIVSDG SEQSESDRKLVVVRGTVETRSSVEGN KSLQPNVLISQESGDRAVIIQRTQV
Sbjct: 61  RHIVSDGNSEQSESDRKLVVVRGTVETRSSVEGNWKSLQPNVLISQESGDRAVIIQRTQV 120

Query: 121 CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLLEGHSTYSDFVVVNMEGSRHP 180
           CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVL++GHSTYSDFVVVNMEGSRHP
Sbjct: 121 CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLVDGHSTYSDFVVVNMEGSRHP 180

Query: 181 LPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKNISAVGICSFENGVPV 240
           LPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKNISAVGICSFENGVPV
Sbjct: 181 LPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKNISAVGICSFENGVPV 240

Query: 241 IKSCSDFPHFLCEMTKDQMILDLVFKTKFLFWSSIVLGSLTVGILGYSAARNWNRWKQWR 300
           IKSCSDFPHFLCEMTKDQMILDLVFKTKFLFWSSIVLGSLTVGILGYSA RNWNRWKQWR
Sbjct: 241 IKSCSDFPHFLCEMTKDQMILDLVFKTKFLFWSSIVLGSLTVGILGYSAVRNWNRWKQWR 300

Query: 301 QHRQLQNSRNDSPTSQLDSSNDIPIYGDFASDDEAATVPDDELSSHVPDDELSSHVPDGQ 360
           QHR+LQNSRNDS TSQLDSSNDIPIYGDFASDDEAAT         VPDDELSS+VPDGQ
Sbjct: 301 QHRRLQNSRNDSTTSQLDSSNDIPIYGDFASDDEAAT---------VPDDELSSNVPDGQ 360

Query: 361 LCVICLMRRKRSAFIPCGHLVCCERCAVSVERESSPKCPICRQQIRSSVRIYDS 415
           LCVICLMRRKRSAFIPCGHLVCCERCAVSVER+SSPKCPICRQQIRSSVRIYDS
Sbjct: 361 LCVICLMRRKRSAFIPCGHLVCCERCAVSVERDSSPKCPICRQQIRSSVRIYDS 405

[Close]

BLAST of CSPI07G22530 vs. NCBI nr
Match: XP_038897215.1 (E3 ubiquitin-protein ligase SPL2 [Benincasa hispida])

HSP 1 Score: 731.9 bits (1888), Expect = 3.1e-207
Identity = 371/416 (89.18%), Postives = 381/416 (91.59%), Query Frame = 0


Query: 1   MSTSEQALVTLLSRFALSFDGAVLGAALAYATVRTVLKLTASCSALNELRKAPYVTVSDL 60
           MSTSEQALVTLLSRFALSFDGAVLGAALAYA VRTVLKLTASCSALNELRKAPYV VSDL
Sbjct: 1   MSTSEQALVTLLSRFALSFDGAVLGAALAYAAVRTVLKLTASCSALNELRKAPYVGVSDL 60

Query: 61  RHIVSDGVSEQSESDRKLVVVRGTVETRSSVEGNRKSLQPNVLISQESGDRAVIIQRTQV 120
           RHI+ DG S QSESDRKLVVVRGTVE RSSVEGN KSLQPN+LISQESGDRAVIIQRTQV
Sbjct: 61  RHIIPDGDSGQSESDRKLVVVRGTVEARSSVEGNWKSLQPNILISQESGDRAVIIQRTQV 120

Query: 121 CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLLEGHSTYSDFVVVNMEGSRHP 180
           CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVL+EGHS YSDFVVVNM+GSRHP
Sbjct: 121 CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLVEGHSPYSDFVVVNMDGSRHP 180

Query: 181 LPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKNISAVGICSFENGVPV 240
           LPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKNISAVGICSFENGVPV
Sbjct: 181 LPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKNISAVGICSFENGVPV 240

Query: 241 IKSCSDFPHFLCEMTKDQMILDLVFKTKFLFWSSIVLGSLTVGILGYSAARNWNRWKQWR 300
           IKSCSDFPHFLCEMTKDQMILDLVFKTKFLFWSSIVLGSL+VGILGYS  RNWNRWKQWR
Sbjct: 241 IKSCSDFPHFLCEMTKDQMILDLVFKTKFLFWSSIVLGSLSVGILGYSVVRNWNRWKQWR 300

Query: 301 QHRQLQNSRNDSPTSQLDSSNDIPIYGDF--ASDDEAATVPDDELSSHVPDDELSSHVPD 360
           QHR+LQNS NDS TSQLDSSNDIPIYGDF  ASDDE AT                  VPD
Sbjct: 301 QHRRLQNSGNDSTTSQLDSSNDIPIYGDFGTASDDEVAT------------------VPD 360

Query: 361 GQLCVICLMRRKRSAFIPCGHLVCCERCAVSVERESSPKCPICRQQIRSSVRIYDS 415
           GQLCVICL RRKRSAFIPCGH VCCERCAVS+ER+S+PKCP+CRQQIRSSVRIYDS
Sbjct: 361 GQLCVICLTRRKRSAFIPCGHFVCCERCAVSIERDSAPKCPVCRQQIRSSVRIYDS 398

[Close]

BLAST of CSPI07G22530 vs. NCBI nr
Match: XP_023549225.1 (LOW QUALITY PROTEIN: E3 ubiquitin-protein ligase SPL2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 706.4 bits (1822), Expect = 1.4e-199
Identity = 359/417 (86.09%), Postives = 384/417 (92.09%), Query Frame = 0


Query: 1   MSTSEQALVTLLSRFALSFDGAVLGAALAYATVRTVLKLTASCSALNELRKAPYVTVSDL 60
           MS+SEQALV+LLS+FAL FDGAVLGAALAYA VRTVLKLTASCSAL+ELRKAPYV+VSDL
Sbjct: 1   MSSSEQALVSLLSQFALXFDGAVLGAALAYAAVRTVLKLTASCSALDELRKAPYVSVSDL 60

Query: 61  RHIVSDGVSEQSESDRKLVVVRGTVETRSSVEGNRKSLQPNVLISQESGDRAVIIQRTQV 120
           RHI+SDG S QSESD KLVVVRGTVE RSSVEGN KS+QPNVLISQESGDRAVIIQRTQ+
Sbjct: 61  RHIISDGDSGQSESDHKLVVVRGTVEARSSVEGNWKSVQPNVLISQESGDRAVIIQRTQM 120

Query: 121 CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLL-EGHSTYSDFVVVNMEGSRH 180
           CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVL+ EGHS YSDFVVVNM+GSRH
Sbjct: 121 CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLVDEGHSPYSDFVVVNMDGSRH 180

Query: 181 PLPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKNISAVGICSFENGVP 240
           PLPLTTVYHQLQPV ATPYTFLQA+FGHEYPVGVLDEEKILPLGKNISAVGICSF+NGVP
Sbjct: 181 PLPLTTVYHQLQPVSATPYTFLQALFGHEYPVGVLDEEKILPLGKNISAVGICSFKNGVP 240

Query: 241 VIKSCSDFPHFLCEMTKDQMILDLVFKTKFLFWSSIVLGSLTVGILGYSAARNWNRWKQW 300
           VIKSC DFPHFLCEMTKDQMILDLVFKTKFLFWSS+VLGSL+VG+LGYS  RNWNRWKQW
Sbjct: 241 VIKSCKDFPHFLCEMTKDQMILDLVFKTKFLFWSSVVLGSLSVGVLGYSVVRNWNRWKQW 300

Query: 301 RQHRQLQNSRNDSPTSQLDSSNDIPIYGDF--ASDDEAATVPDDELSSHVPDDELSSHVP 360
           RQ R+LQ+S NDS  SQLD SNDIPIYGDF  ASD+E A           P+DE+SS+VP
Sbjct: 301 RQQRRLQDSGNDSTMSQLD-SNDIPIYGDFGTASDNEEAA---------DPEDEVSSNVP 360

Query: 361 DGQLCVICLMRRKRSAFIPCGHLVCCERCAVSVERESSPKCPICRQQIRSSVRIYDS 415
           DGQLCVICLMRRKRSAFIPCGH VCCERCA+SVER+S+PKCP+CRQQIRSSVRIYDS
Sbjct: 361 DGQLCVICLMRRKRSAFIPCGHFVCCERCAMSVERDSAPKCPVCRQQIRSSVRIYDS 407

[Close]

BLAST of CSPI07G22530 vs. NCBI nr
Match: XP_022992269.1 (E3 ubiquitin-protein ligase SPL2 [Cucurbita maxima] >XP_022992271.1 E3 ubiquitin-protein ligase SPL2 [Cucurbita maxima])

HSP 1 Score: 704.9 bits (1818), Expect = 4.1e-199
Identity = 358/417 (85.85%), Postives = 385/417 (92.33%), Query Frame = 0


Query: 1   MSTSEQALVTLLSRFALSFDGAVLGAALAYATVRTVLKLTASCSALNELRKAPYVTVSDL 60
           MS+SEQALV+LLS+FALSFDGAVLGAALAYA VRTVLKLTASCSAL+ELRKAPYV+VSDL
Sbjct: 1   MSSSEQALVSLLSQFALSFDGAVLGAALAYAAVRTVLKLTASCSALDELRKAPYVSVSDL 60

Query: 61  RHIVSDGVSEQSESDRKLVVVRGTVETRSSVEGNRKSLQPNVLISQESGDRAVIIQRTQV 120
           RHI+SDG S +SESD KLVVVRGTVE RSSVEGN KS+QPNVLISQESGDRAVIIQRTQ+
Sbjct: 61  RHIISDGDSGKSESDHKLVVVRGTVEARSSVEGNWKSVQPNVLISQESGDRAVIIQRTQM 120

Query: 121 CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLL-EGHSTYSDFVVVNMEGSRH 180
           CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVL+ EGHS YSDFVVVNM+GSRH
Sbjct: 121 CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLVDEGHSPYSDFVVVNMDGSRH 180

Query: 181 PLPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKNISAVGICSFENGVP 240
           PLPLTTVYHQLQPV ATPYTFLQA+FGHEYPVGVLDEEKILPLGKNISAVGICSF+NGVP
Sbjct: 181 PLPLTTVYHQLQPVSATPYTFLQALFGHEYPVGVLDEEKILPLGKNISAVGICSFKNGVP 240

Query: 241 VIKSCSDFPHFLCEMTKDQMILDLVFKTKFLFWSSIVLGSLTVGILGYSAARNWNRWKQW 300
           VIKSC DFPHFLCEMTKDQMILDLVFKTKFLFWSS+VLGSL+VG+LGYS  RNWNRWKQW
Sbjct: 241 VIKSCKDFPHFLCEMTKDQMILDLVFKTKFLFWSSVVLGSLSVGVLGYSVVRNWNRWKQW 300

Query: 301 RQHRQLQNSRNDSPTSQLDSSNDIPIYGDF--ASDDEAATVPDDELSSHVPDDELSSHVP 360
           RQ R+LQ+S NDS  SQLD SN+IPIYGDF  ASD+E A           P+DE+SS+VP
Sbjct: 301 RQQRRLQDSGNDSTMSQLD-SNNIPIYGDFGTASDNEEAV---------DPEDEVSSNVP 360

Query: 361 DGQLCVICLMRRKRSAFIPCGHLVCCERCAVSVERESSPKCPICRQQIRSSVRIYDS 415
           DGQLCVICLMRRKRSAFIPCGH VCCERCA+SVER+S+PKCP+CRQQIRSSVRIYDS
Sbjct: 361 DGQLCVICLMRRKRSAFIPCGHFVCCERCAMSVERDSAPKCPVCRQQIRSSVRIYDS 407

[Close]

BLAST of CSPI07G22530 vs. TAIR 10
Match: AT1G54150.1 (E3 Ubiquitin ligase family protein )

HSP 1 Score: 362.5 bits (929), Expect = 4.7e-100
Identity = 204/421 (48.46%), Postives = 279/421 (66.27%), Query Frame = 0


Query: 1   MSTSEQALVTLLSRFALSFDGAVLGAALAYATVRTVLKLTASCSALNELRKAPYVTVSDL 60
           MS+ E+AL+ LL+  ALSFDGA+LG  LA + V + LK  ++ +AL +++ AP V++SDL
Sbjct: 1   MSSPERALLNLLTDIALSFDGAILGLTLAVSAVGSALKYASTNAALKKIKDAPEVSISDL 60

Query: 61  RHIV--SDGVSEQSE----SDRKLVVVRGTVETRSSVEGNRKSLQPNVLISQESGDRAVI 120
           R ++  S+  SE ++    +D+++VVVRG V+ +  + G+      NVLIS E+GD+A+I
Sbjct: 61  RSLLPASEDKSETNDNRKSNDQRIVVVRGVVKPK--ISGDEGYKNNNVLISPETGDKALI 120

Query: 121 IQRTQVCIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLL-EGHSTYSDFVVVN 180
           IQRTQ  +++ WK  F  T   R +  R+ RK  +   RTVPFV++ +   + S FV VN
Sbjct: 121 IQRTQTYVYSGWKRLFQSTGH-RFMLERSLRKHGADFTRTVPFVIVGKDQQSNSSFVAVN 180

Query: 181 MEGSRHPLPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKNISAVGICS 240
           M+GSR PLPLTTVY++LQP+ +   +FLQA    +YPVG+LD EKILP GK+I+AVGI S
Sbjct: 181 MDGSRQPLPLTTVYNRLQPINS---SFLQAFLYPDYPVGLLDIEKILPPGKDITAVGIYS 240

Query: 241 FENGVPVIKSCSDFPHFLCEMTKDQMILDLVFKTKFLFWSSIVLGSLTVGILGYSAARNW 300
           F NGVP IKSC D P+FL EMTKD+MI DL+ +T F+F  S++LG ++VGIL Y+A R W
Sbjct: 241 FNNGVPEIKSCQDLPYFLSEMTKDKMIEDLMEQTNFIFLGSVILGIVSVGILSYAAVRTW 300

Query: 301 NRWKQWRQHRQLQNSRNDSPTSQLDSSNDIPIYGDFASDDEAATVPDDELSSHVPDDELS 360
           N+WKQW   R+L    NDS                         V DDE     P+D  +
Sbjct: 301 NKWKQWNHQRELPQRPNDS-------------------------VVDDE-----PED--A 360

Query: 361 SHVPDGQLCVICLMRRKRSAFIPCGHLVCCERCAVSVERESSPKCPICRQQIRSSVRIYD 415
             +PDG+LCVIC+ RR+  AFIPCGH+VCC RCA +VERE +PKCP+C Q IR S+R+Y 
Sbjct: 361 DEIPDGELCVICVSRRRVPAFIPCGHVVCCRRCASTVERELNPKCPVCLQSIRGSMRVYY 383

[Close]

BLAST of CSPI07G22530 vs. TAIR 10
Match: AT4G24204.1 (RING/U-box superfamily protein )

HSP 1 Score: 59.3 bits (142), Expect = 8.4e-09
Identity = 27/59 (45.76%), Postives = 32/59 (54.24%), Query Frame = 0


Query: 353 SSHVPDGQLCVICLMRRKRSAFIPCGHLVCCERCAVSVERESSPKCPICRQQIRSSVRI 412
           S  V    LCVIC   R+   F+PCGH   C  CA  +  E S  CPICR+ IR S R+
Sbjct: 60  SDDVDYSTLCVICFEERRNCFFVPCGHSATCRGCAQRILSEESKVCPICRRVIRKSKRL 118

[Close]

BLAST of CSPI07G22530 vs. TAIR 10
Match: AT4G24204.2 (RING/U-box superfamily protein )

HSP 1 Score: 59.3 bits (142), Expect = 8.4e-09
Identity = 27/59 (45.76%), Postives = 32/59 (54.24%), Query Frame = 0


Query: 353 SSHVPDGQLCVICLMRRKRSAFIPCGHLVCCERCAVSVERESSPKCPICRQQIRSSVRI 412
           S  V    LCVIC   R+   F+PCGH   C  CA  +  E S  CPICR+ IR S R+
Sbjct: 94  SDDVDYSTLCVICFEERRNCFFVPCGHSATCRGCAQRILSEESKVCPICRRVIRKSKRL 152

[Close]

BLAST of CSPI07G22530 vs. TAIR 10
Match: AT4G24204.3 (RING/U-box superfamily protein )

HSP 1 Score: 59.3 bits (142), Expect = 8.4e-09
Identity = 27/59 (45.76%), Postives = 32/59 (54.24%), Query Frame = 0


Query: 353 SSHVPDGQLCVICLMRRKRSAFIPCGHLVCCERCAVSVERESSPKCPICRQQIRSSVRI 412
           S  V    LCVIC   R+   F+PCGH   C  CA  +  E S  CPICR+ IR S R+
Sbjct: 115 SDDVDYSTLCVICFEERRNCFFVPCGHSATCRGCAQRILSEESKVCPICRRVIRKSKRL 173

[Close]

BLAST of CSPI07G22530 vs. TAIR 10
Match: AT1G59560.1 (E3 Ubiquitin ligase family protein )

HSP 1 Score: 53.9 bits (128), Expect = 3.5e-07
Identity = 20/52 (38.46%), Postives = 35/52 (67.31%), Query Frame = 0


Query: 361 LCVICLMRRKRSAFIPCGHLVCCERCAVSVERESSPKCPICRQQIRSSVRIY 413
           LCV+CL ++  +AF+ CGH+ CC  C++ +       CP+CR++I+  ++IY
Sbjct: 290 LCVVCLDQKYNTAFVECGHMCCCTPCSLQLR-----TCPLCRERIQQVLKIY 336

The following BLAST results are available for this feature:

BLAST of CSPI07G22530 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SYH3	6.6e-99	48.46	E3 ubiquitin-protein ligase SPL2 OS=Arabidopsis thaliana OX=3702 GN=SPL2 PE=2 SV...	[more]
P41437	2.2e-09	39.24	E3 ubiquitin-protein ligase IAP-3 OS=Orgyia pseudotsugata multicapsid polyhedros...	[more]
O08863	5.3e-08	33.33	Baculoviral IAP repeat-containing protein 3 OS=Mus musculus OX=10090 GN=Birc3 PE...	[more]
P41436	9.1e-08	36.49	Apoptosis inhibitor IAP OS=Cydia pomonella granulosis virus (isolate Mexico/1963...	[more]
Q24306	1.2e-07	41.94	Death-associated inhibitor of apoptosis 1 OS=Drosophila melanogaster OX=7227 GN=...	[more]

BLAST of CSPI07G22530 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K708	2.0e-228	97.83	RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_7G450490 PE...	[more]
A0A5D3BWQ0	5.7e-223	95.65	RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A1S3CGP0	5.7e-223	95.65	RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103500694 PE=4 ...	[more]
A0A6J1JT39	2.0e-199	85.85	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111488642 P...	[more]
A0A6J1HK84	1.3e-198	84.54	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111464304...	[more]

BLAST of CSPI07G22530 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011659622.2	2.5e-233	97.87	E3 ubiquitin-protein ligase SPL2 isoform X1 [Cucumis sativus]	[more]
XP_008462309.1	1.2e-222	95.65	PREDICTED: mitochondrial ubiquitin ligase activator of nfkb 1 [Cucumis melo] >KA...	[more]
XP_038897215.1	3.1e-207	89.18	E3 ubiquitin-protein ligase SPL2 [Benincasa hispida]	[more]
XP_023549225.1	1.4e-199	86.09	LOW QUALITY PROTEIN: E3 ubiquitin-protein ligase SPL2 [Cucurbita pepo subsp. pep...	[more]
XP_022992269.1	4.1e-199	85.85	E3 ubiquitin-protein ligase SPL2 [Cucurbita maxima] >XP_022992271.1 E3 ubiquitin...	[more]

BLAST of CSPI07G22530 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G54150.1	4.7e-100	48.46	E3 Ubiquitin ligase family protein	[more]
AT4G24204.1	8.4e-09	45.76	RING/U-box superfamily protein	[more]
AT4G24204.2	8.4e-09	45.76	RING/U-box superfamily protein	[more]
AT4G24204.3	8.4e-09	45.76	RING/U-box superfamily protein	[more]
AT1G59560.1	3.5e-07	38.46	E3 Ubiquitin ligase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR022170	E3 Ubiquitin ligase MUL1-like	PFAM	PF12483	GIDE	coord: 142..283 e-value: 1.4E-33 score: 115.6
None	No IPR available	PANTHER	PTHR47355:SF1	E3 UBIQUITIN-PROTEIN LIGASE SPL2	coord: 1..291
IPR044247	E3 ubiquitin-protein ligase SPL2-like	PANTHER	PTHR47355	E3 UBIQUITIN-PROTEIN LIGASE SPL2	coord: 1..291

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI07G22530.1	CSPI07G22530.1	mRNA
CSPI07G22530.2	CSPI07G22530.2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006996	organelle organization
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0009941	chloroplast envelope
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016874	ligase activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0004842	ubiquitin-protein transferase activity