CSPI07G22020 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI07G22020
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionRING-type E3 ubiquitin transferase
LocationChr7: 18707252 .. 18708515 (+)
RNA-Seq ExpressionCSPI07G22020
SyntenyCSPI07G22020
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AACAAACACTTTCTTCTTCTTCTTCTTCTTCTTCATCTTCTTCTTCCACTTTCTCTCCATAAACACAACCAAAAAACAAAAGTCCACCCCAAAGTCTTCCCTCATTTCTTCTTTAAATCTCTCCTTCTTCATCTTCTCATTTCAACATTTCCCAAATCCCAAAATCCCATTACTGATCATTTCCAGTTTCGGCCATTCTCATGGAGGAACAAGCATCCACAATCAATCATCCCTTCGGCGACTCCATTGCAATGGAGCTCACAGGCAAAATCATGCTCGCCGCTATTTTAATTCTCTGTTTAGTCATTGCCTTCGTTCTCCTTCTCCAGCTTTACTCTCGTTGGTTCTTATCTCGCCTTCACCAATCCTCCTCTGATTCCGCCACAAATCAAGAATCCCCTGTTTCCACTACATTGCGCAAGGGTCTTGATTCCGCCGTCCTCCACTCAATTCCGGTGGTGGTGTTCTCGCCGGCGGATTTCAAAGAGGGATTAGAATGTGCTGTGTGTTTGTCGGAGTTAAGTGAAGGGGAGAAAGCTCGGTTGCTTCCGAGATGTAACCATGGCTTCCATGTCGATTGTATTGATATGTGGTTCAAATCAAATTCCACCTGCCCTCTTTGTAGAAACCCAGTGGCAATCACAGAACCGAATTCAGAACAGAATCCAGAAACTGGGTCTTCAATGGAGTCTCCGATTTTTCCTACTAATGTTCTGTTTTGGGGGAATCAAATGCAGGTTAGTTCGAGAGGGGTTTGTTTAGAAGAACCTCAGATTTCTTCTTCTTCTTCTTCTACTTCTTCTTCATTAAATACAATGGTGGTAATTGACATTCCAAACGAGCCCTCTACATCCGGCGCCGGCACTTGTTTCGCCGATGAAGAGATGGGATCGGTTGTGACGAACAGATTGAGAACATTGAAAAGGCTTTTGAGCAGAGAGCGAAGAATTGGGACCAATCCCATTCCTTCAGATATTGAACAGGAAGGGATAGTGCAAAGCTAAACAGAATTAAGAAAGAAAATCGATTATGGTTTTTTAAAATCTTTTGCACATATAGAAGGTTAGAAGAATCGGATTTTTTTCTTTTTGAAATTGTTTTTGAGTTTCAAGTCAGAGACTGTGAGGTTCATAGTCCGGCTGTTACCATTGTAAAACTTGAATGTGGATGAAAACAACAAAATTGATTGTAATGTGAAAAACAAGAAGTAAGTTTGGTTTATGTTAAATTTGAGAAATGGAAAGAGAGTTCATCTTCTTGGGTC

mRNA sequence

AACAAACACTTTCTTCTTCTTCTTCTTCTTCTTCATCTTCTTCTTCCACTTTCTCTCCATAAACACAACCAAAAAACAAAAGTCCACCCCAAAGTCTTCCCTCATTTCTTCTTTAAATCTCTCCTTCTTCATCTTCTCATTTCAACATTTCCCAAATCCCAAAATCCCATTACTGATCATTTCCAGTTTCGGCCATTCTCATGGAGGAACAAGCATCCACAATCAATCATCCCTTCGGCGACTCCATTGCAATGGAGCTCACAGGCAAAATCATGCTCGCCGCTATTTTAATTCTCTGTTTAGTCATTGCCTTCGTTCTCCTTCTCCAGCTTTACTCTCGTTGGTTCTTATCTCGCCTTCACCAATCCTCCTCTGATTCCGCCACAAATCAAGAATCCCCTGTTTCCACTACATTGCGCAAGGGTCTTGATTCCGCCGTCCTCCACTCAATTCCGGTGGTGGTGTTCTCGCCGGCGGATTTCAAAGAGGGATTAGAATGTGCTGTGTGTTTGTCGGAGTTAAGTGAAGGGGAGAAAGCTCGGTTGCTTCCGAGATGTAACCATGGCTTCCATGTCGATTGTATTGATATGTGGTTCAAATCAAATTCCACCTGCCCTCTTTGTAGAAACCCAGTGGCAATCACAGAACCGAATTCAGAACAGAATCCAGAAACTGGGTCTTCAATGGAGTCTCCGATTTTTCCTACTAATGTTCTGTTTTGGGGGAATCAAATGCAGGTTAGTTCGAGAGGGGTTTGTTTAGAAGAACCTCAGATTTCTTCTTCTTCTTCTTCTACTTCTTCTTCATTAAATACAATGGTGGTAATTGACATTCCAAACGAGCCCTCTACATCCGGCGCCGGCACTTGTTTCGCCGATGAAGAGATGGGATCGGTTGTGACGAACAGATTGAGAACATTGAAAAGGCTTTTGAGCAGAGAGCGAAGAATTGGGACCAATCCCATTCCTTCAGATATTGAACAGGAAGGGATAGTGCAAAGCTAAACAGAATTAAGAAAGAAAATCGATTATGGTTTTTTAAAATCTTTTGCACATATAGAAGGTTAGAAGAATCGGATTTTTTTCTTTTTGAAATTGTTTTTGAGTTTCAAGTCAGAGACTGTGAGGTTCATAGTCCGGCTGTTACCATTGTAAAACTTGAATGTGGATGAAAACAACAAAATTGATTGTAATGTGAAAAACAAGAAGTAAGTTTGGTTTATGTTAAATTTGAGAAATGGAAAGAGAGTTCATCTTCTTGGGTC

Coding sequence (CDS)

ATGGAGGAACAAGCATCCACAATCAATCATCCCTTCGGCGACTCCATTGCAATGGAGCTCACAGGCAAAATCATGCTCGCCGCTATTTTAATTCTCTGTTTAGTCATTGCCTTCGTTCTCCTTCTCCAGCTTTACTCTCGTTGGTTCTTATCTCGCCTTCACCAATCCTCCTCTGATTCCGCCACAAATCAAGAATCCCCTGTTTCCACTACATTGCGCAAGGGTCTTGATTCCGCCGTCCTCCACTCAATTCCGGTGGTGGTGTTCTCGCCGGCGGATTTCAAAGAGGGATTAGAATGTGCTGTGTGTTTGTCGGAGTTAAGTGAAGGGGAGAAAGCTCGGTTGCTTCCGAGATGTAACCATGGCTTCCATGTCGATTGTATTGATATGTGGTTCAAATCAAATTCCACCTGCCCTCTTTGTAGAAACCCAGTGGCAATCACAGAACCGAATTCAGAACAGAATCCAGAAACTGGGTCTTCAATGGAGTCTCCGATTTTTCCTACTAATGTTCTGTTTTGGGGGAATCAAATGCAGGTTAGTTCGAGAGGGGTTTGTTTAGAAGAACCTCAGATTTCTTCTTCTTCTTCTTCTACTTCTTCTTCATTAAATACAATGGTGGTAATTGACATTCCAAACGAGCCCTCTACATCCGGCGCCGGCACTTGTTTCGCCGATGAAGAGATGGGATCGGTTGTGACGAACAGATTGAGAACATTGAAAAGGCTTTTGAGCAGAGAGCGAAGAATTGGGACCAATCCCATTCCTTCAGATATTGAACAGGAAGGGATAGTGCAAAGCTAA

Protein sequence

MEEQASTINHPFGDSIAMELTGKIMLAAILILCLVIAFVLLLQLYSRWFLSRLHQSSSDSATNQESPVSTTLRKGLDSAVLHSIPVVVFSPADFKEGLECAVCLSELSEGEKARLLPRCNHGFHVDCIDMWFKSNSTCPLCRNPVAITEPNSEQNPETGSSMESPIFPTNVLFWGNQMQVSSRGVCLEEPQISSSSSSTSSSLNTMVVIDIPNEPSTSGAGTCFADEEMGSVVTNRLRTLKRLLSRERRIGTNPIPSDIEQEGIVQS*
Homology
BLAST of CSPI07G22020 vs. ExPASy Swiss-Prot
Match: P0C035 (RING-H2 finger protein ATL60 OS=Arabidopsis thaliana OX=3702 GN=ATL60 PE=2 SV=1)

HSP 1 Score: 181.4 bits (459), Expect = 1.3e-44
Identity = 124/310 (40.00%), Postives = 162/310 (52.26%), Query Frame = 0

Query: 1   MEEQASTINHPFGDSIAMELTGKIMLAAILILCLVIAFVLLLQLYSRWFLSRLHQSSSDS 60
           M+E++ +    F      E  GK++L +I+ +   I F+LLL LY+R F  R+ Q  + +
Sbjct: 1   MDEESVSNGSLFSKFEGEETMGKVLLFSIVSIFTGILFLLLLHLYARLFWWRVEQHFNLN 60

Query: 61  ATNQESPVSTTLRK--------------------GLDSAVLHSIPVVVFSPADFKEGLEC 120
               + P ST + +                    GLDS +L SI VVVF   DFK+GLEC
Sbjct: 61  LIQSDDPGSTVIGRNPRRRRFVFAQSQEDPLHNAGLDSKILQSIHVVVFKCTDFKDGLEC 120

Query: 121 AVCLSELSEGEKARLLPRCNHGFHVDCIDMWFKSNSTCPLCRNPVAITEPNS-------- 180
           AVCLS+L +G+KAR+LPRCNHGFHVDCIDMWF+S+STCPLCRN V   E  +        
Sbjct: 121 AVCLSDLVDGDKARVLPRCNHGFHVDCIDMWFQSHSTCPLCRNTVGSVEDTTHGGSEGLP 180

Query: 181 -EQNPETGS-----------------SMESPIFPTNVLFWGNQMQVSSRGVCLEEPQISS 240
             QN E+G                  S E   FPTNVL WG+Q QV S G+ + E   S 
Sbjct: 181 QNQNFESGHSTNQHNPSQDQSFVHEFSTEPLSFPTNVLVWGDQNQVRSAGLVVTEESPSG 240

Query: 241 S-----------SSSTSSSLNTMVVIDIPNEPSTSGAGTCFADEEMGSVVTNRLRTLKRL 254
           +           SSST S   T VV+DIP+  S+        +EE  S +  RLR LK +
Sbjct: 241 NFAASYNDHQQESSSTRSQEVTAVVVDIPDN-SSENLSERIDEEEPKSPMFTRLRLLKNV 300

BLAST of CSPI07G22020 vs. ExPASy Swiss-Prot
Match: Q9XF63 (RING-H2 finger protein ATL3 OS=Arabidopsis thaliana OX=3702 GN=ATL3 PE=2 SV=1)

HSP 1 Score: 175.6 bits (444), Expect = 7.3e-43
Identity = 115/305 (37.70%), Postives = 163/305 (53.44%), Query Frame = 0

Query: 1   MEEQASTINHPFGDSIAMELTGKIMLAAILILCLVIAFVLLLQLYSR---WFLSRLHQSS 60
           M++  ST +  FGD    E+T KI+L AI++L + + FVL+L LY++   W + +L Q  
Sbjct: 1   MDDNRSTHSSMFGDLSTEEVTSKIILTAIIVLFMAVLFVLILHLYAKLYWWRIDQLQQQQ 60

Query: 61  SDSATNQES---------PVSTTLRK---------------GLDSAVLHSIPVVVFSPAD 120
                 QE          PV T  ++               GL S  L S+P+V F    
Sbjct: 61  QQQQQEQEQEEDQSSIAPPVVTRRQRRRFIFVPGQDALSNTGLTSFELSSLPIVFFRQDS 120

Query: 121 FKEGLECAVCLSELSEGEKARLLPRCNHGFHVDCIDMWFKSNSTCPLCRNPVAITE---- 180
            K+GLEC++CLSEL +G+KARLLP+CNH FHV+CIDMWF+S+STCP+CRN V   E    
Sbjct: 121 CKDGLECSICLSELVKGDKARLLPKCNHSFHVECIDMWFQSHSTCPICRNTVLGPEQASS 180

Query: 181 ------PNSEQNPETGS---------SMESPIFPTNVLFWGNQMQVSSRGVCLEEPQISS 240
                 P++ +N  T +         S  SP FPTNVL WG Q QVS+    +   +  +
Sbjct: 181 KRVEQVPDNAENAGTTNNNHDALSQLSTSSPEFPTNVLVWGRQDQVSTGNTNVGTQEDGA 240

Query: 241 SSSSTSSSLNTMVVIDIPN--------EPSTSGAGTCFADEEMGSVVTNRLRTLKRLLSR 252
           + +  S S    VV+DI +          S+S       +EE  S +T RLR+L+R LSR
Sbjct: 241 AGNGASQS-QEAVVLDISDSSSRNHNVSSSSSSMRFIVEEEEAKSPMTTRLRSLRRFLSR 300

BLAST of CSPI07G22020 vs. ExPASy Swiss-Prot
Match: Q8L9T5 (RING-H2 finger protein ATL2 OS=Arabidopsis thaliana OX=3702 GN=ATL2 PE=2 SV=2)

HSP 1 Score: 124.0 bits (310), Expect = 2.5e-27
Identity = 94/271 (34.69%), Postives = 134/271 (49.45%), Query Frame = 0

Query: 12  FGDSIAMELTGKIMLAAILILCLVIAFVLLLQLYSRWFLSR-----LHQSSSD------- 71
           F  S    ++GKIML+AI+IL  V+  ++ L LY+RW+L R     L + S +       
Sbjct: 18  FSGSKTYAMSGKIMLSAIVILFFVVILMVFLHLYARWYLLRARRRHLRRRSRNRRATMVF 77

Query: 72  -SATNQESPVSTTLRKGLDSAVLHSIPVVVFSPADFKEGLECAVCLSELSEGEKARLLPR 131
            +A    +  S    +GLD  V+ S+PV  FS    K+ +ECAVCLSE  E E  R+LP 
Sbjct: 78  FTADPSTAATSVVASRGLDPNVIKSLPVFTFSDETHKDPIECAVCLSEFEESETGRVLPN 137

Query: 132 CNHGFHVDCIDMWFKSNSTCPLCRNPV----AITEPNSEQNPETGSSMESPIFPTNVLFW 191
           C H FHVDCIDMWF S+STCPLCR+ V     I    + +  E   +++S   P  V+  
Sbjct: 138 CQHTFHVDCIDMWFHSHSTCPLCRSLVESLAGIESTAAAREREVVIAVDSD--PVLVI-- 197

Query: 192 GNQMQVSSRGVCLEEPQISSSSSS-TSSSLNTMVVIDIPNEPSTSGAGTCFADE------ 251
               + SS     +EP  S SS      S      I++P    +      F DE      
Sbjct: 198 ----EPSSSSGLTDEPHGSGSSQMLREDSGRKPAAIEVPRRTFSE-----FEDELTRRDS 257

Query: 252 ---EMGSVVTNRLRTLKRLLSRERRIGTNPI 256
              +      +R+ +  R+LSR+RR  ++PI
Sbjct: 258 PASQSFRSPMSRMLSFTRMLSRDRRSASSPI 275

BLAST of CSPI07G22020 vs. ExPASy Swiss-Prot
Match: O22255 (RING-H2 finger protein ATL64 OS=Arabidopsis thaliana OX=3702 GN=ATL64 PE=2 SV=1)

HSP 1 Score: 115.9 bits (289), Expect = 6.9e-25
Identity = 66/155 (42.58%), Postives = 92/155 (59.35%), Query Frame = 0

Query: 20  LTGKIMLAAILILCLVIAFVLLLQLYSRWFLSRLHQSSSDSATNQESPVSTTLR-KGLDS 79
           L GKIML+++++L + +  +L    Y+RW   R ++       +    +S + R + LD 
Sbjct: 24  LNGKIMLSSVIVLFVAVIMILCFHSYARWLFRRHNRRIRRRIRSHLRTLSASPRDQALDQ 83

Query: 80  AVLHSIPVVVFS---PADFKEGLECAVCLSELSEGEKARLLPRCNHGFHVDCIDMWFKSN 139
           AVL  IP+ V+S   P   +E  EC+VCLSE  E ++ RLLP+C H FHVDCID WF+S 
Sbjct: 84  AVLDKIPIFVYSSKNPPPPEEKEECSVCLSEFEEEDEGRLLPKCGHSFHVDCIDTWFRSR 143

Query: 140 STCPLCRNPVAITEPNSEQNPETGSSMESPI-FPT 170
           STCPLCR PV   +P  +      SS  SP+ FPT
Sbjct: 144 STCPLCRAPV---QPPFQVIETGSSSSSSPLTFPT 175

BLAST of CSPI07G22020 vs. ExPASy Swiss-Prot
Match: Q9LZJ6 (RING-H2 finger protein ATL5 OS=Arabidopsis thaliana OX=3702 GN=ATL5 PE=2 SV=1)

HSP 1 Score: 109.8 bits (273), Expect = 4.9e-23
Identity = 81/246 (32.93%), Postives = 121/246 (49.19%), Query Frame = 0

Query: 20  LTGKIMLAAILILCLVIAFVLLLQLYSRWFL--------SRLHQSSSDSATNQESPVSTT 79
           L GKIMLA+++IL + +  +L    Y+RW           R+       A  ++   S++
Sbjct: 24  LNGKIMLASVIILFVAVILILCFHSYARWLFRRQNRRIRRRISAHLRSLAAARDPTQSSS 83

Query: 80  LRKGLDSAVLHSIPVVVFSPADFKEGL-ECAVCLSELSEGEKARLLPRCNHGFHVDCIDM 139
               LD  VL  IP+ V+S    +  L EC+VCLSE  E ++ R+LP+C H FHVDCID 
Sbjct: 84  SLSPLDPTVLEKIPIFVYSVKTHESPLEECSVCLSEFEEDDEGRVLPKCGHVFHVDCIDT 143

Query: 140 WFKSNSTCPLCRNPVAITEPNSEQNPETGSSMESPIFPTNVLFWGNQMQVSSRGVCLEEP 199
           WF+S S+CPLCR PV   +P +E  P       + +FP+       +   SS     +E 
Sbjct: 144 WFRSRSSCPLCRAPVQPAQPVTEPEP------VAAVFPSVKPIEDTEAGSSSSS---DES 203

Query: 200 QISSSSSSTSSSLN-------------TMVVIDIPNEPSTSGAGTCFADEEMGSVVTNRL 244
           + S+ SSS+ S +                ++++IP E   S +       + GS   NR 
Sbjct: 204 ESSTPSSSSGSPVRFPMEACEREPIDLVGIIVEIPREFQDSNSD---LPADNGS---NRR 254

BLAST of CSPI07G22020 vs. ExPASy TrEMBL
Match: A0A0A0K6T2 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_7G447970 PE=3 SV=1)

HSP 1 Score: 522.7 bits (1345), Expect = 9.0e-145
Identity = 265/267 (99.25%), Postives = 267/267 (100.00%), Query Frame = 0

Query: 1   MEEQASTINHPFGDSIAMELTGKIMLAAILILCLVIAFVLLLQLYSRWFLSRLHQSSSDS 60
           MEEQASTI+HPFGDSIAMELTGKIMLAAILILCLVIAFVLLLQLYSRWFLSRLHQSSSDS
Sbjct: 1   MEEQASTISHPFGDSIAMELTGKIMLAAILILCLVIAFVLLLQLYSRWFLSRLHQSSSDS 60

Query: 61  ATNQESPVSTTLRKGLDSAVLHSIPVVVFSPADFKEGLECAVCLSELSEGEKARLLPRCN 120
           ATNQESPVSTTLRKGLDSAVLHSIPVVVFSPADFKEGLECAVCLSELSEGEKARLLPRCN
Sbjct: 61  ATNQESPVSTTLRKGLDSAVLHSIPVVVFSPADFKEGLECAVCLSELSEGEKARLLPRCN 120

Query: 121 HGFHVDCIDMWFKSNSTCPLCRNPVAITEPNSEQNPETGSSMESPIFPTNVLFWGNQMQV 180
           HGFHVDCIDMWFKSNSTCPLCRNPVAITEPNSEQNPETGSSMESPIFPTNVLFWGNQMQV
Sbjct: 121 HGFHVDCIDMWFKSNSTCPLCRNPVAITEPNSEQNPETGSSMESPIFPTNVLFWGNQMQV 180

Query: 181 SSRGVCLEEPQISSSSSSTSSSLNTMVVIDIPNEPSTSGAGTCFADEEMGSVVTNRLRTL 240
           SSRGVCLEEPQISSSSSSTSSS+NTMVVIDIPNEPSTSGAGTCFADEEMGSVVTNRLRTL
Sbjct: 181 SSRGVCLEEPQISSSSSSTSSSINTMVVIDIPNEPSTSGAGTCFADEEMGSVVTNRLRTL 240

Query: 241 KRLLSRERRIGTNPIPSDIEQEGIVQS 268
           KRLLSRERRIGTNPIPSDIEQEGIVQS
Sbjct: 241 KRLLSRERRIGTNPIPSDIEQEGIVQS 267

BLAST of CSPI07G22020 vs. ExPASy TrEMBL
Match: A0A5D3BW46 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G001700 PE=3 SV=1)

HSP 1 Score: 485.3 bits (1248), Expect = 1.6e-133
Identity = 250/270 (92.59%), Postives = 254/270 (94.07%), Query Frame = 0

Query: 1   MEEQASTINHPFGDSIAMELTGKIMLAAILILCLVIAFVLLLQLYSRWFLSRLHQSSSDS 60
           ME QASTINHPFGDS AM+LTGKIM AAILILCLVIAFVLLLQLYSRWFLSRLHQSSSDS
Sbjct: 1   MEAQASTINHPFGDSFAMQLTGKIMFAAILILCLVIAFVLLLQLYSRWFLSRLHQSSSDS 60

Query: 61  ATNQESPVSTTLRKGLDSAVLHSIPVVVFSPADFKEGLECAVCLSELSEGEKARLLPRCN 120
           ATNQESPVSTTLRKGLDSAVLHSIPVVVFSPADFKEGLECAVCLSELSEGEKARLLPRCN
Sbjct: 61  ATNQESPVSTTLRKGLDSAVLHSIPVVVFSPADFKEGLECAVCLSELSEGEKARLLPRCN 120

Query: 121 HGFHVDCIDMWFKSNSTCPLCRNPVAITEPNSEQNPETGSSMESPIFPTNVLFWGNQMQV 180
           HGFHVDCIDMWFKSNSTCPLCRNPVAITEPN EQNPET SS ESPIFPTNVLFWGNQMQV
Sbjct: 121 HGFHVDCIDMWFKSNSTCPLCRNPVAITEPNLEQNPETESSTESPIFPTNVLFWGNQMQV 180

Query: 181 SSRGVCLEEPQISSSSS---STSSSLNTMVVIDIPNEPSTSGAGTCFADEEMGSVVTNRL 240
           SSRGVCLEEPQISSSSS   S+ SS N MVVIDIP++PSTSGA TCFADEEMGSVVTNRL
Sbjct: 181 SSRGVCLEEPQISSSSSSSPSSPSSANAMVVIDIPSQPSTSGASTCFADEEMGSVVTNRL 240

Query: 241 RTLKRLLSRERRIGTNPIPSDIEQEGIVQS 268
           RTLKRLLSRERRIG NPI SDIEQEGI QS
Sbjct: 241 RTLKRLLSRERRIGNNPISSDIEQEGIGQS 270

BLAST of CSPI07G22020 vs. ExPASy TrEMBL
Match: A0A1S3CH08 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103500641 PE=3 SV=1)

HSP 1 Score: 485.3 bits (1248), Expect = 1.6e-133
Identity = 250/270 (92.59%), Postives = 254/270 (94.07%), Query Frame = 0

Query: 1   MEEQASTINHPFGDSIAMELTGKIMLAAILILCLVIAFVLLLQLYSRWFLSRLHQSSSDS 60
           ME QASTINHPFGDS AM+LTGKIM AAILILCLVIAFVLLLQLYSRWFLSRLHQSSSDS
Sbjct: 1   MEAQASTINHPFGDSFAMQLTGKIMFAAILILCLVIAFVLLLQLYSRWFLSRLHQSSSDS 60

Query: 61  ATNQESPVSTTLRKGLDSAVLHSIPVVVFSPADFKEGLECAVCLSELSEGEKARLLPRCN 120
           ATNQESPVSTTLRKGLDSAVLHSIPVVVFSPADFKEGLECAVCLSELSEGEKARLLPRCN
Sbjct: 61  ATNQESPVSTTLRKGLDSAVLHSIPVVVFSPADFKEGLECAVCLSELSEGEKARLLPRCN 120

Query: 121 HGFHVDCIDMWFKSNSTCPLCRNPVAITEPNSEQNPETGSSMESPIFPTNVLFWGNQMQV 180
           HGFHVDCIDMWFKSNSTCPLCRNPVAITEPN EQNPET SS ESPIFPTNVLFWGNQMQV
Sbjct: 121 HGFHVDCIDMWFKSNSTCPLCRNPVAITEPNLEQNPETESSTESPIFPTNVLFWGNQMQV 180

Query: 181 SSRGVCLEEPQISSSSS---STSSSLNTMVVIDIPNEPSTSGAGTCFADEEMGSVVTNRL 240
           SSRGVCLEEPQISSSSS   S+ SS N MVVIDIP++PSTSGA TCFADEEMGSVVTNRL
Sbjct: 181 SSRGVCLEEPQISSSSSSSPSSPSSANAMVVIDIPSQPSTSGASTCFADEEMGSVVTNRL 240

Query: 241 RTLKRLLSRERRIGTNPIPSDIEQEGIVQS 268
           RTLKRLLSRERRIG NPI SDIEQEGI QS
Sbjct: 241 RTLKRLLSRERRIGNNPISSDIEQEGIGQS 270

BLAST of CSPI07G22020 vs. ExPASy TrEMBL
Match: A0A6J1HKG4 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111464366 PE=3 SV=1)

HSP 1 Score: 360.1 bits (923), Expect = 7.8e-96
Identity = 201/282 (71.28%), Postives = 226/282 (80.14%), Query Frame = 0

Query: 1   MEEQASTINHPFGDSIAMELTGKIMLAAILILCLVIAFVLLLQLYSRWFLSRLHQSSSDS 60
           MEE AS I+  F  S+ MEL GKIM AAI IL LVI FVL+LQLYSRW LSR+H+SSSDS
Sbjct: 1   MEEHASGISKSFEGSLTMELMGKIMAAAIFILFLVIVFVLILQLYSRWLLSRIHESSSDS 60

Query: 61  A----TNQESPVSTTLRKGLDSAVLHSIPVVVFSPADFKEGLECAVCLSELSEGEKARLL 120
           A    ++QES V+ TL KGLD+AVL  IPVVVFSP DFKEGLECAVCLSEL++GEK RLL
Sbjct: 61  AVLRRSDQESDVA-TLTKGLDAAVLRLIPVVVFSPEDFKEGLECAVCLSELAQGEKVRLL 120

Query: 121 PRCNHGFHVDCIDMWFKSNSTCPLCRNPVA-----ITEPNSEQNPETGSSMESPIFPTNV 180
           PRC+HGFHVDCIDMWFKSNSTCPLCR+PVA      TE NS ++ E+GSSMESPIFPTN+
Sbjct: 121 PRCSHGFHVDCIDMWFKSNSTCPLCRDPVAPKPSIPTEMNSAEDLESGSSMESPIFPTNI 180

Query: 181 LFWGNQMQVSSRGVCLEEPQISSSSSSTSSSLNT----MVVIDIPNEPSTSGAGTC--FA 240
           LFWGNQMQ+SSRGVCLEE Q SSSSSS+SSS N     MVVIDIPNEPS+S +GT    A
Sbjct: 181 LFWGNQMQISSRGVCLEEAQTSSSSSSSSSSSNIRADGMVVIDIPNEPSSSSSGTSGRSA 240

Query: 241 DEEMGSVVTNRLRTLKRLLSRERRIGTNPIPSDIEQEGIVQS 268
            EEM SVVT+RL+TLKRLLSRERRIG N    ++EQ G  QS
Sbjct: 241 GEEMKSVVTSRLKTLKRLLSRERRIGNNLTSLNLEQAGRAQS 281

BLAST of CSPI07G22020 vs. ExPASy TrEMBL
Match: A0A6J1D188 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111016097 PE=3 SV=1)

HSP 1 Score: 352.1 bits (902), Expect = 2.1e-93
Identity = 193/277 (69.68%), Postives = 220/277 (79.42%), Query Frame = 0

Query: 1   MEEQASTINHPFGDSIAMELTGKIMLAAILILCLVIAFVLLLQLYSRWFLSRLHQSSSDS 60
           MEE  S I  PFG S AMELTGKIMLAAILIL LVI FVL+LQLYSRWFLSR+ +S+ DS
Sbjct: 1   MEEHTSRITRPFGGSFAMELTGKIMLAAILILFLVIIFVLVLQLYSRWFLSRIVESTPDS 60

Query: 61  ATNQESPVSTTLRKGLDSAVLHSIPVVVFSPADFKEGLECAVCLSELSEGEKARLLPRCN 120
           A +  S    +LRKGLD+AVL S+PV+VF  ADF+EGLECAVCLSEL++GE+ARLLPRCN
Sbjct: 61  AASHGSG-QDSLRKGLDAAVLRSLPVLVFEAADFEEGLECAVCLSELAQGERARLLPRCN 120

Query: 121 HGFHVDCIDMWFKSNSTCPLCRNPVAI-----TEPNSEQNPETGSSMESPIFPTNVLFWG 180
           HGFHVDCIDMWFKSNSTCPLCRNPVA+     ++ NS ++ E+GSS+ESPIFPTNVLFWG
Sbjct: 121 HGFHVDCIDMWFKSNSTCPLCRNPVALQALNPSQSNSAEDLESGSSIESPIFPTNVLFWG 180

Query: 181 NQMQVSSRGVCLEEPQISSSSSSTSSSLNTMVVIDIPNEPSTS----GAGTCFADEEMGS 240
           NQMQVSSRGVCLEE   SSSS++T    + +V IDIPNEPS S    GAG C  DEEM S
Sbjct: 181 NQMQVSSRGVCLEEAPTSSSSANTRP--DGVVAIDIPNEPSASSSGAGAGGCTGDEEMKS 240

Query: 241 VVTNRLRTLKRLLSRERRIGTNPI-PSDIEQEGIVQS 268
            +T RLRTLKRLLS ERR G N + PSD+EQ G  QS
Sbjct: 241 ALTTRLRTLKRLLSGERRAGGNSLTPSDLEQVGRGQS 274

BLAST of CSPI07G22020 vs. NCBI nr
Match: XP_004141825.2 (RING-H2 finger protein ATL60 [Cucumis sativus] >KGN45435.1 hypothetical protein Csa_016496 [Cucumis sativus])

HSP 1 Score: 522.7 bits (1345), Expect = 1.9e-144
Identity = 265/267 (99.25%), Postives = 267/267 (100.00%), Query Frame = 0

Query: 1   MEEQASTINHPFGDSIAMELTGKIMLAAILILCLVIAFVLLLQLYSRWFLSRLHQSSSDS 60
           MEEQASTI+HPFGDSIAMELTGKIMLAAILILCLVIAFVLLLQLYSRWFLSRLHQSSSDS
Sbjct: 1   MEEQASTISHPFGDSIAMELTGKIMLAAILILCLVIAFVLLLQLYSRWFLSRLHQSSSDS 60

Query: 61  ATNQESPVSTTLRKGLDSAVLHSIPVVVFSPADFKEGLECAVCLSELSEGEKARLLPRCN 120
           ATNQESPVSTTLRKGLDSAVLHSIPVVVFSPADFKEGLECAVCLSELSEGEKARLLPRCN
Sbjct: 61  ATNQESPVSTTLRKGLDSAVLHSIPVVVFSPADFKEGLECAVCLSELSEGEKARLLPRCN 120

Query: 121 HGFHVDCIDMWFKSNSTCPLCRNPVAITEPNSEQNPETGSSMESPIFPTNVLFWGNQMQV 180
           HGFHVDCIDMWFKSNSTCPLCRNPVAITEPNSEQNPETGSSMESPIFPTNVLFWGNQMQV
Sbjct: 121 HGFHVDCIDMWFKSNSTCPLCRNPVAITEPNSEQNPETGSSMESPIFPTNVLFWGNQMQV 180

Query: 181 SSRGVCLEEPQISSSSSSTSSSLNTMVVIDIPNEPSTSGAGTCFADEEMGSVVTNRLRTL 240
           SSRGVCLEEPQISSSSSSTSSS+NTMVVIDIPNEPSTSGAGTCFADEEMGSVVTNRLRTL
Sbjct: 181 SSRGVCLEEPQISSSSSSTSSSINTMVVIDIPNEPSTSGAGTCFADEEMGSVVTNRLRTL 240

Query: 241 KRLLSRERRIGTNPIPSDIEQEGIVQS 268
           KRLLSRERRIGTNPIPSDIEQEGIVQS
Sbjct: 241 KRLLSRERRIGTNPIPSDIEQEGIVQS 267

BLAST of CSPI07G22020 vs. NCBI nr
Match: XP_008462235.1 (PREDICTED: RING-H2 finger protein ATL60-like [Cucumis melo] >KAA0059353.1 RING-H2 finger protein ATL60-like [Cucumis melo var. makuwa] >TYK03973.1 RING-H2 finger protein ATL60-like [Cucumis melo var. makuwa])

HSP 1 Score: 485.3 bits (1248), Expect = 3.3e-133
Identity = 250/270 (92.59%), Postives = 254/270 (94.07%), Query Frame = 0

Query: 1   MEEQASTINHPFGDSIAMELTGKIMLAAILILCLVIAFVLLLQLYSRWFLSRLHQSSSDS 60
           ME QASTINHPFGDS AM+LTGKIM AAILILCLVIAFVLLLQLYSRWFLSRLHQSSSDS
Sbjct: 1   MEAQASTINHPFGDSFAMQLTGKIMFAAILILCLVIAFVLLLQLYSRWFLSRLHQSSSDS 60

Query: 61  ATNQESPVSTTLRKGLDSAVLHSIPVVVFSPADFKEGLECAVCLSELSEGEKARLLPRCN 120
           ATNQESPVSTTLRKGLDSAVLHSIPVVVFSPADFKEGLECAVCLSELSEGEKARLLPRCN
Sbjct: 61  ATNQESPVSTTLRKGLDSAVLHSIPVVVFSPADFKEGLECAVCLSELSEGEKARLLPRCN 120

Query: 121 HGFHVDCIDMWFKSNSTCPLCRNPVAITEPNSEQNPETGSSMESPIFPTNVLFWGNQMQV 180
           HGFHVDCIDMWFKSNSTCPLCRNPVAITEPN EQNPET SS ESPIFPTNVLFWGNQMQV
Sbjct: 121 HGFHVDCIDMWFKSNSTCPLCRNPVAITEPNLEQNPETESSTESPIFPTNVLFWGNQMQV 180

Query: 181 SSRGVCLEEPQISSSSS---STSSSLNTMVVIDIPNEPSTSGAGTCFADEEMGSVVTNRL 240
           SSRGVCLEEPQISSSSS   S+ SS N MVVIDIP++PSTSGA TCFADEEMGSVVTNRL
Sbjct: 181 SSRGVCLEEPQISSSSSSSPSSPSSANAMVVIDIPSQPSTSGASTCFADEEMGSVVTNRL 240

Query: 241 RTLKRLLSRERRIGTNPIPSDIEQEGIVQS 268
           RTLKRLLSRERRIG NPI SDIEQEGI QS
Sbjct: 241 RTLKRLLSRERRIGNNPISSDIEQEGIGQS 270

BLAST of CSPI07G22020 vs. NCBI nr
Match: XP_038897361.1 (RING-H2 finger protein ATL60-like [Benincasa hispida])

HSP 1 Score: 444.1 bits (1141), Expect = 8.4e-121
Identity = 228/267 (85.39%), Postives = 239/267 (89.51%), Query Frame = 0

Query: 1   MEEQASTINHPFGDSIAMELTGKIMLAAILILCLVIAFVLLLQLYSRWFLSRLHQSSSDS 60
           MEE ASTIN PFG S AMELTGKIMLAAILILCLVIAFVL+LQLYSRWFLS +HQSS DS
Sbjct: 1   MEEHASTINRPFGGSFAMELTGKIMLAAILILCLVIAFVLILQLYSRWFLSHIHQSSPDS 60

Query: 61  ATNQESPVSTTLRKGLDSAVLHSIPVVVFSPADFKEGLECAVCLSELSEGEKARLLPRCN 120
           A N ESPVS+TL KGLDSA+L SIPVVVFSPADF+EGLECAVCLSELSEGEKARLLP CN
Sbjct: 61  AANHESPVSSTLHKGLDSALLQSIPVVVFSPADFEEGLECAVCLSELSEGEKARLLPICN 120

Query: 121 HGFHVDCIDMWFKSNSTCPLCRNPVAITEPNSEQNPETGSSMESPIFPTNVLFWGNQMQV 180
           HGFHVDCIDMWFKSNSTCPLCRNPV  TE NSE N E+GSSMESPIFPTNVLFWGNQMQV
Sbjct: 121 HGFHVDCIDMWFKSNSTCPLCRNPVIPTESNSEHNLESGSSMESPIFPTNVLFWGNQMQV 180

Query: 181 SSRGVCLEEPQISSSSSSTSSSLNTMVVIDIPNEPSTSGAGTCFADEEMGSVVTNRLRTL 240
           SSRG+CLEE QISSSS++T +  N MVVIDIPNEPSTSGAG CFADEE GSVVTNRLRTL
Sbjct: 181 SSRGICLEESQISSSSANTRADGNGMVVIDIPNEPSTSGAGGCFADEETGSVVTNRLRTL 240

Query: 241 KRLLSRERRIGTNPIPSDIEQEGIVQS 268
           KRLLSRERRIG N   SD+EQ GIVQS
Sbjct: 241 KRLLSRERRIGNNLTSSDLEQVGIVQS 267

BLAST of CSPI07G22020 vs. NCBI nr
Match: KAG6593587.1 (RING-H2 finger protein ATL60, partial [Cucurbita argyrosperma subsp. sororia] >KAG7025931.1 RING-H2 finger protein ATL60, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 361.3 bits (926), Expect = 7.2e-96
Identity = 202/281 (71.89%), Postives = 226/281 (80.43%), Query Frame = 0

Query: 1   MEEQASTINHPFGDSIAMELTGKIMLAAILILCLVIAFVLLLQLYSRWFLSRLHQSSSDS 60
           MEE AS I+  F  S+ MEL GKIM AAI IL LVI FVL+LQLYSRW LSR+H+SSSDS
Sbjct: 1   MEEHASGISKSFEGSLTMELMGKIMAAAIFILFLVIVFVLILQLYSRWLLSRIHESSSDS 60

Query: 61  A----TNQESPVSTTLRKGLDSAVLHSIPVVVFSPADFKEGLECAVCLSELSEGEKARLL 120
           A    ++QES V+ TL KGLD+AVL  IPVVVFSP DFKEGLECAVCLSEL++GEKARLL
Sbjct: 61  AVLRRSDQESDVA-TLTKGLDAAVLRLIPVVVFSPEDFKEGLECAVCLSELAQGEKARLL 120

Query: 121 PRCNHGFHVDCIDMWFKSNSTCPLCRNPVA-----ITEPNSEQNPETGSSMESPIFPTNV 180
           PRCNHGFHVDCIDMWFKSNSTCPLCR+PVA      TE NS ++ E+GSSMESPIFPTN+
Sbjct: 121 PRCNHGFHVDCIDMWFKSNSTCPLCRDPVAPKPSIPTEMNSAEDLESGSSMESPIFPTNI 180

Query: 181 LFWGNQMQVSSRGVCLEEPQISSSSSSTSSSLNT----MVVIDIPNEPSTSGAGT--CFA 240
           LFWGNQMQ+SSRGVCLEE Q SSSSSS+SSS N     MVVIDIPNEPS+S +GT    A
Sbjct: 181 LFWGNQMQISSRGVCLEEAQTSSSSSSSSSSSNIRADGMVVIDIPNEPSSSSSGTGGRSA 240

Query: 241 DEEMGSVVTNRLRTLKRLLSRERRIGTNPIPSDIEQEGIVQ 267
            EEM SVVT+RL+TLKRLLSRERRIG N    ++EQ G  Q
Sbjct: 241 GEEMKSVVTSRLKTLKRLLSRERRIGNNLTSLNLEQAGRAQ 280

BLAST of CSPI07G22020 vs. NCBI nr
Match: XP_022964315.1 (RING-H2 finger protein ATL60-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 360.1 bits (923), Expect = 1.6e-95
Identity = 201/282 (71.28%), Postives = 226/282 (80.14%), Query Frame = 0

Query: 1   MEEQASTINHPFGDSIAMELTGKIMLAAILILCLVIAFVLLLQLYSRWFLSRLHQSSSDS 60
           MEE AS I+  F  S+ MEL GKIM AAI IL LVI FVL+LQLYSRW LSR+H+SSSDS
Sbjct: 1   MEEHASGISKSFEGSLTMELMGKIMAAAIFILFLVIVFVLILQLYSRWLLSRIHESSSDS 60

Query: 61  A----TNQESPVSTTLRKGLDSAVLHSIPVVVFSPADFKEGLECAVCLSELSEGEKARLL 120
           A    ++QES V+ TL KGLD+AVL  IPVVVFSP DFKEGLECAVCLSEL++GEK RLL
Sbjct: 61  AVLRRSDQESDVA-TLTKGLDAAVLRLIPVVVFSPEDFKEGLECAVCLSELAQGEKVRLL 120

Query: 121 PRCNHGFHVDCIDMWFKSNSTCPLCRNPVA-----ITEPNSEQNPETGSSMESPIFPTNV 180
           PRC+HGFHVDCIDMWFKSNSTCPLCR+PVA      TE NS ++ E+GSSMESPIFPTN+
Sbjct: 121 PRCSHGFHVDCIDMWFKSNSTCPLCRDPVAPKPSIPTEMNSAEDLESGSSMESPIFPTNI 180

Query: 181 LFWGNQMQVSSRGVCLEEPQISSSSSSTSSSLNT----MVVIDIPNEPSTSGAGTC--FA 240
           LFWGNQMQ+SSRGVCLEE Q SSSSSS+SSS N     MVVIDIPNEPS+S +GT    A
Sbjct: 181 LFWGNQMQISSRGVCLEEAQTSSSSSSSSSSSNIRADGMVVIDIPNEPSSSSSGTSGRSA 240

Query: 241 DEEMGSVVTNRLRTLKRLLSRERRIGTNPIPSDIEQEGIVQS 268
            EEM SVVT+RL+TLKRLLSRERRIG N    ++EQ G  QS
Sbjct: 241 GEEMKSVVTSRLKTLKRLLSRERRIGNNLTSLNLEQAGRAQS 281

BLAST of CSPI07G22020 vs. TAIR 10
Match: AT1G53820.1 (RING/U-box superfamily protein )

HSP 1 Score: 181.4 bits (459), Expect = 9.5e-46
Identity = 124/310 (40.00%), Postives = 162/310 (52.26%), Query Frame = 0

Query: 1   MEEQASTINHPFGDSIAMELTGKIMLAAILILCLVIAFVLLLQLYSRWFLSRLHQSSSDS 60
           M+E++ +    F      E  GK++L +I+ +   I F+LLL LY+R F  R+ Q  + +
Sbjct: 1   MDEESVSNGSLFSKFEGEETMGKVLLFSIVSIFTGILFLLLLHLYARLFWWRVEQHFNLN 60

Query: 61  ATNQESPVSTTLRK--------------------GLDSAVLHSIPVVVFSPADFKEGLEC 120
               + P ST + +                    GLDS +L SI VVVF   DFK+GLEC
Sbjct: 61  LIQSDDPGSTVIGRNPRRRRFVFAQSQEDPLHNAGLDSKILQSIHVVVFKCTDFKDGLEC 120

Query: 121 AVCLSELSEGEKARLLPRCNHGFHVDCIDMWFKSNSTCPLCRNPVAITEPNS-------- 180
           AVCLS+L +G+KAR+LPRCNHGFHVDCIDMWF+S+STCPLCRN V   E  +        
Sbjct: 121 AVCLSDLVDGDKARVLPRCNHGFHVDCIDMWFQSHSTCPLCRNTVGSVEDTTHGGSEGLP 180

Query: 181 -EQNPETGS-----------------SMESPIFPTNVLFWGNQMQVSSRGVCLEEPQISS 240
             QN E+G                  S E   FPTNVL WG+Q QV S G+ + E   S 
Sbjct: 181 QNQNFESGHSTNQHNPSQDQSFVHEFSTEPLSFPTNVLVWGDQNQVRSAGLVVTEESPSG 240

Query: 241 S-----------SSSTSSSLNTMVVIDIPNEPSTSGAGTCFADEEMGSVVTNRLRTLKRL 254
           +           SSST S   T VV+DIP+  S+        +EE  S +  RLR LK +
Sbjct: 241 NFAASYNDHQQESSSTRSQEVTAVVVDIPDN-SSENLSERIDEEEPKSPMFTRLRLLKNV 300

BLAST of CSPI07G22020 vs. TAIR 10
Match: AT1G72310.1 (RING/U-box superfamily protein )

HSP 1 Score: 175.6 bits (444), Expect = 5.2e-44
Identity = 115/305 (37.70%), Postives = 163/305 (53.44%), Query Frame = 0

Query: 1   MEEQASTINHPFGDSIAMELTGKIMLAAILILCLVIAFVLLLQLYSR---WFLSRLHQSS 60
           M++  ST +  FGD    E+T KI+L AI++L + + FVL+L LY++   W + +L Q  
Sbjct: 1   MDDNRSTHSSMFGDLSTEEVTSKIILTAIIVLFMAVLFVLILHLYAKLYWWRIDQLQQQQ 60

Query: 61  SDSATNQES---------PVSTTLRK---------------GLDSAVLHSIPVVVFSPAD 120
                 QE          PV T  ++               GL S  L S+P+V F    
Sbjct: 61  QQQQQEQEQEEDQSSIAPPVVTRRQRRRFIFVPGQDALSNTGLTSFELSSLPIVFFRQDS 120

Query: 121 FKEGLECAVCLSELSEGEKARLLPRCNHGFHVDCIDMWFKSNSTCPLCRNPVAITE---- 180
            K+GLEC++CLSEL +G+KARLLP+CNH FHV+CIDMWF+S+STCP+CRN V   E    
Sbjct: 121 CKDGLECSICLSELVKGDKARLLPKCNHSFHVECIDMWFQSHSTCPICRNTVLGPEQASS 180

Query: 181 ------PNSEQNPETGS---------SMESPIFPTNVLFWGNQMQVSSRGVCLEEPQISS 240
                 P++ +N  T +         S  SP FPTNVL WG Q QVS+    +   +  +
Sbjct: 181 KRVEQVPDNAENAGTTNNNHDALSQLSTSSPEFPTNVLVWGRQDQVSTGNTNVGTQEDGA 240

Query: 241 SSSSTSSSLNTMVVIDIPN--------EPSTSGAGTCFADEEMGSVVTNRLRTLKRLLSR 252
           + +  S S    VV+DI +          S+S       +EE  S +T RLR+L+R LSR
Sbjct: 241 AGNGASQS-QEAVVLDISDSSSRNHNVSSSSSSMRFIVEEEEAKSPMTTRLRSLRRFLSR 300

BLAST of CSPI07G22020 vs. TAIR 10
Match: AT3G16720.1 (TOXICOS EN LEVADURA 2 )

HSP 1 Score: 124.0 bits (310), Expect = 1.8e-28
Identity = 94/271 (34.69%), Postives = 134/271 (49.45%), Query Frame = 0

Query: 12  FGDSIAMELTGKIMLAAILILCLVIAFVLLLQLYSRWFLSR-----LHQSSSD------- 71
           F  S    ++GKIML+AI+IL  V+  ++ L LY+RW+L R     L + S +       
Sbjct: 18  FSGSKTYAMSGKIMLSAIVILFFVVILMVFLHLYARWYLLRARRRHLRRRSRNRRATMVF 77

Query: 72  -SATNQESPVSTTLRKGLDSAVLHSIPVVVFSPADFKEGLECAVCLSELSEGEKARLLPR 131
            +A    +  S    +GLD  V+ S+PV  FS    K+ +ECAVCLSE  E E  R+LP 
Sbjct: 78  FTADPSTAATSVVASRGLDPNVIKSLPVFTFSDETHKDPIECAVCLSEFEESETGRVLPN 137

Query: 132 CNHGFHVDCIDMWFKSNSTCPLCRNPV----AITEPNSEQNPETGSSMESPIFPTNVLFW 191
           C H FHVDCIDMWF S+STCPLCR+ V     I    + +  E   +++S   P  V+  
Sbjct: 138 CQHTFHVDCIDMWFHSHSTCPLCRSLVESLAGIESTAAAREREVVIAVDSD--PVLVI-- 197

Query: 192 GNQMQVSSRGVCLEEPQISSSSSS-TSSSLNTMVVIDIPNEPSTSGAGTCFADE------ 251
               + SS     +EP  S SS      S      I++P    +      F DE      
Sbjct: 198 ----EPSSSSGLTDEPHGSGSSQMLREDSGRKPAAIEVPRRTFSE-----FEDELTRRDS 257

Query: 252 ---EMGSVVTNRLRTLKRLLSRERRIGTNPI 256
              +      +R+ +  R+LSR+RR  ++PI
Sbjct: 258 PASQSFRSPMSRMLSFTRMLSRDRRSASSPI 275

BLAST of CSPI07G22020 vs. TAIR 10
Match: AT2G47560.1 (RING/U-box superfamily protein )

HSP 1 Score: 115.9 bits (289), Expect = 4.9e-26
Identity = 66/155 (42.58%), Postives = 92/155 (59.35%), Query Frame = 0

Query: 20  LTGKIMLAAILILCLVIAFVLLLQLYSRWFLSRLHQSSSDSATNQESPVSTTLR-KGLDS 79
           L GKIML+++++L + +  +L    Y+RW   R ++       +    +S + R + LD 
Sbjct: 24  LNGKIMLSSVIVLFVAVIMILCFHSYARWLFRRHNRRIRRRIRSHLRTLSASPRDQALDQ 83

Query: 80  AVLHSIPVVVFS---PADFKEGLECAVCLSELSEGEKARLLPRCNHGFHVDCIDMWFKSN 139
           AVL  IP+ V+S   P   +E  EC+VCLSE  E ++ RLLP+C H FHVDCID WF+S 
Sbjct: 84  AVLDKIPIFVYSSKNPPPPEEKEECSVCLSEFEEEDEGRLLPKCGHSFHVDCIDTWFRSR 143

Query: 140 STCPLCRNPVAITEPNSEQNPETGSSMESPI-FPT 170
           STCPLCR PV   +P  +      SS  SP+ FPT
Sbjct: 144 STCPLCRAPV---QPPFQVIETGSSSSSSPLTFPT 175

BLAST of CSPI07G22020 vs. TAIR 10
Match: AT3G62690.1 (AtL5 )

HSP 1 Score: 109.8 bits (273), Expect = 3.5e-24
Identity = 81/246 (32.93%), Postives = 121/246 (49.19%), Query Frame = 0

Query: 20  LTGKIMLAAILILCLVIAFVLLLQLYSRWFL--------SRLHQSSSDSATNQESPVSTT 79
           L GKIMLA+++IL + +  +L    Y+RW           R+       A  ++   S++
Sbjct: 24  LNGKIMLASVIILFVAVILILCFHSYARWLFRRQNRRIRRRISAHLRSLAAARDPTQSSS 83

Query: 80  LRKGLDSAVLHSIPVVVFSPADFKEGL-ECAVCLSELSEGEKARLLPRCNHGFHVDCIDM 139
               LD  VL  IP+ V+S    +  L EC+VCLSE  E ++ R+LP+C H FHVDCID 
Sbjct: 84  SLSPLDPTVLEKIPIFVYSVKTHESPLEECSVCLSEFEEDDEGRVLPKCGHVFHVDCIDT 143

Query: 140 WFKSNSTCPLCRNPVAITEPNSEQNPETGSSMESPIFPTNVLFWGNQMQVSSRGVCLEEP 199
           WF+S S+CPLCR PV   +P +E  P       + +FP+       +   SS     +E 
Sbjct: 144 WFRSRSSCPLCRAPVQPAQPVTEPEP------VAAVFPSVKPIEDTEAGSSSSS---DES 203

Query: 200 QISSSSSSTSSSLN-------------TMVVIDIPNEPSTSGAGTCFADEEMGSVVTNRL 244
           + S+ SSS+ S +                ++++IP E   S +       + GS   NR 
Sbjct: 204 ESSTPSSSSGSPVRFPMEACEREPIDLVGIIVEIPREFQDSNSD---LPADNGS---NRR 254

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P0C0351.3e-4440.00RING-H2 finger protein ATL60 OS=Arabidopsis thaliana OX=3702 GN=ATL60 PE=2 SV=1[more]
Q9XF637.3e-4337.70RING-H2 finger protein ATL3 OS=Arabidopsis thaliana OX=3702 GN=ATL3 PE=2 SV=1[more]
Q8L9T52.5e-2734.69RING-H2 finger protein ATL2 OS=Arabidopsis thaliana OX=3702 GN=ATL2 PE=2 SV=2[more]
O222556.9e-2542.58RING-H2 finger protein ATL64 OS=Arabidopsis thaliana OX=3702 GN=ATL64 PE=2 SV=1[more]
Q9LZJ64.9e-2332.93RING-H2 finger protein ATL5 OS=Arabidopsis thaliana OX=3702 GN=ATL5 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0K6T29.0e-14599.25RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_7G447970 PE... [more]
A0A5D3BW461.6e-13392.59RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3CH081.6e-13392.59RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103500641 PE=3 ... [more]
A0A6J1HKG47.8e-9671.28RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111464366... [more]
A0A6J1D1882.1e-9369.68RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11101609... [more]
Match NameE-valueIdentityDescription
XP_004141825.21.9e-14499.25RING-H2 finger protein ATL60 [Cucumis sativus] >KGN45435.1 hypothetical protein ... [more]
XP_008462235.13.3e-13392.59PREDICTED: RING-H2 finger protein ATL60-like [Cucumis melo] >KAA0059353.1 RING-H... [more]
XP_038897361.18.4e-12185.39RING-H2 finger protein ATL60-like [Benincasa hispida][more]
KAG6593587.17.2e-9671.89RING-H2 finger protein ATL60, partial [Cucurbita argyrosperma subsp. sororia] >K... [more]
XP_022964315.11.6e-9571.28RING-H2 finger protein ATL60-like isoform X1 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT1G53820.19.5e-4640.00RING/U-box superfamily protein [more]
AT1G72310.15.2e-4437.70RING/U-box superfamily protein [more]
AT3G16720.11.8e-2834.69TOXICOS EN LEVADURA 2 [more]
AT2G47560.14.9e-2642.58RING/U-box superfamily protein [more]
AT3G62690.13.5e-2432.93AtL5 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 100..141
e-value: 2.5E-8
score: 43.7
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 99..142
e-value: 7.6E-14
score: 51.8
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 100..142
score: 12.607302
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 77..150
e-value: 1.7E-20
score: 74.0
IPR044675E3 ubiquitin-protein ligase RING1-likePANTHERPTHR45676RING-H2 FINGER PROTEIN ATL51-RELATEDcoord: 4..255
NoneNo IPR availablePANTHERPTHR45676:SF132RING-H2 FINGER PROTEIN ATL3coord: 4..255
NoneNo IPR availableCDDcd16461RING-H2_EL5_likecoord: 99..142
e-value: 2.38415E-18
score: 74.2687
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 80..148

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI07G22020.1CSPI07G22020.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0016021 integral component of membrane