CSPI07G21490 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI07G21490
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionHydroxyphenylpyruvate reductase
LocationChr7: 18353014 .. 18356151 (-)
RNA-Seq ExpressionCSPI07G21490
SyntenyCSPI07G21490
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTTCTTTTCTGTTTGTTTATGCGCATCGAATCTTCCTCTTCTCCACCTTCATCTTCATCATCTAAATTCAACTACATCTCCTCCGAAAACCCTAATCCAACCCCTAAATTCTCAACCATGGAATCCATCGGTGTACTCATGACTTGTCCGATGAACGCTTACCTTGAGGGGGAGCTGCAGAAGCGCTTCAATCTTTACAAGTTCTGGCAATTTCCTCAGAAGACGCAATTCCTCACGGAGCATTGCAATTCGATTCGTGCCGTTGTTGGAAATGCGTCAGCTGGTGCCGATGCTACTTTGATTGATGCCTTGCCGAAGTTGGAGATTGTCTCCAGCTTCAGTGTGGGATTGGATAAGATTGATCTCAAAAAGTGCAAGGAGAAGGGGATTCGGGTCACTAATACTCCCGATGTTTTAACTGAAGACGTTGCGGATTTGGCGATTGGGTTGATTATAGCGGTGCTGAGGAGGTTGTGTGAATGCGATAGATATGTCAGGAGCGGGAAGTGGAAGATTGGTAACTACAAGTTGACTACCAAGGTTCTGTGTTTTTGTGCTTAATTAATGCTACCTGGTATTTTCTTTGGTTGGATATTAATGAAGGATGGGTTTGTTGCTTGGATTTGGTACTATTATCTAACATGTTTAGTTGAAGCATTCTTATCATTTATATTGTTGTTAGTGATAGCAAATTCAAATGGTCGATTGCTTGATTCAATTAAGATGTGGTACTAATACTCAAGTGGTTGAATTCTTGTGGTATTGACTGTGTAGGTTATGTAACCAAGGAAGAAGTTTCCCTAAGACCTGAGTCTACTCGTTGAAATTTTCCAGAAGGTCAAAATTCTGTGTTGTTTTTTCAAAATGCATTGGTTTTCATAATTGCATTATCCTTCCCATTTGTAGATAAGAAGAACTAAGTTCTCCCAATTAATTTTTCTTTGACTGTATACTTAGTTCTGTGGCTTTAGCTAGCAGAGATGTTTCATTTGTGTGAAATAACAATTATCTAGGATTTGGAGATTCTTGCCTGCCTGGAGACAAGAATTTTTTAGTTGATGTAATAATGTTTCATAGTTTCAAACCTAGTGAATGTGTTGCAGTTAGAGATTTTGGGGTGAGAAATTGTTGTTTAGAGAGTAAGTTAGGCTTTTGTTCCTTGATTTGAACTTCTTTTTCACTGTTCATGGGTCGTCAGAACATTCATTTGGTCATTAAGGTTTCAGGGTGACATGTTTGGTCTGGTTGTATTTGATTTATGATTGATCCCGGGAGTTTGATAAAGGTCTCTATGTAGAGTTAACATTGGTTAATATAATGGAATGATGACTAGACTTCGTTGATGTGTATTTTATAAATTAGTTAGACAATTTTTCCTTGTACTTGGGGATCAATTTGAAACTAATTTCAAACTTCGTGGGACTATTATAAATCAACTTTAAATCTTGGCATCAAACAGGTCACTTTTAAAACTTCAATGACCAAATAGAAAAAAAAATCATATCTCAAGAGTTTTAAGTGTTAGGTTTGAAAACTTAGGGACAGTTCAAATCCCAGCTACAAAAGGGTATTTAGCTTTTCCCTTCTGGCAAACCCATTCTTAGGTTGCACCTTGCTTCCCTGACAATTGACTTGAATGAACTTGGGTTTCTGTTTTCTTTTTTGAGCCAAGTGCAGCGTATTTCTTTATTCAAACGTTATTCTATCTAATAGGATCAGTGCAAGAACTTTCACACACACACACACACACACATATTCATTTCTTTCTTTCTATTCTTGCATCTGCAATTAGTTAATGTGTTTGTTCGAAAGTTCATTGATTTTATTTTCAAGTGCTAGAGTTTCCTCTTGCTGTTCATTCTCACTAACAGAACAAGAAATCAGTGCCAATCTTTATATATGTATTTTGGTTTGAACTTCGCCCTGTGCCAATAATGCCCACAGTTAATTATGGGCATGAAGAAAAGGGTGAACATAAGGTTTGAACGTGAAAACTACTTTCTAGTGTTTATGTCTGAACATTTCTTGCTAATGTTCAGAACCAAAGATCACGCAATCAGAAAAGACTTTGGATTCTTTTATCTTTCATAACTTTTTATGTCTTGTTTGGTTATGATGTTGCTACATTGGCAAACATTTTAATTGACCTAACCTATCGATTAGGTATAAAGGGATAAGGTATTCAGTTGTGTCTTTGTAATTATAATTGACCTAACCTATTGATCTATAAAAGTACTAAAAATTGCATTGCTATTGCCTTTCAGTTCTCTGGCAAATCGGTTGGCATTATTGGTTTGGGAAGGATTGGCTTAGCAATTGCCAAGAGAGCTGAAGCATTCAACTGTCCTATATCTTACTACTCAAGAACAAAAAAGGAAGACACCAAGTACAAGTACTACTCAAATCTTCTCGAGTTGGCCTCCAACTCAGATATTCTGATTGTTGCTTGCGCGCTAACTAAAGAAACCCACCACATCGTTAATCGAGAAGTTATTGATGCATTGGGTCCAAAAGGTGTTCTCATCAACATTGGGAGGGGTCCTCACGTCGATGAACCTGAATTAGTAGCTGCCCTGGTTGAAGGCCGATTAGGCGGTGCAGGACTCGATGTGTTTGAAAACGAGCCTGAAGTCCCCCAAGAGCTGTTTGCACTCGAAAATGTTGTCCTGGTGCCGCATATAGGAAGTGGAACTGTTGAAACTCGCAAAGAAATGGCTGACCTTGTGCTTGGAAACCTGGAATCTCACTTCTCCAATAAACCTCTGTTAACTCCAGTGGTATAGTTTTGTGATTGAAACAAATATTAGCCTTCGCTGTTGCCTGGAGAATTCCATGGCATCCTCATCCAGTAACCATCAGACAACACATCGAGTGAACGAATCAATTCGACGAATACTAGCTGGTGATGTTCATAATTTGAATCTTCTATGATTATTGCTTTCATCCCTTACTTGTTGGTATAGTTCAAGTTTTCTTACTCAATCTGATGTAATAGCTTAGCCCTGTTGGTATGATATGTGAAGATATCTTAATAAACGTTGTTGGGGGAAATGCTATCTGTGATATTTGTTTGGTGAAGTCTTCTATTACAATTTGTGTATAATCCAAAACTGAATGGCAAAATTGAAAACTCCTTG

mRNA sequence

ATTTCTTTTCTGTTTGTTTATGCGCATCGAATCTTCCTCTTCTCCACCTTCATCTTCATCATCTAAATTCAACTACATCTCCTCCGAAAACCCTAATCCAACCCCTAAATTCTCAACCATGGAATCCATCGGTGTACTCATGACTTGTCCGATGAACGCTTACCTTGAGGGGGAGCTGCAGAAGCGCTTCAATCTTTACAAGTTCTGGCAATTTCCTCAGAAGACGCAATTCCTCACGGAGCATTGCAATTCGATTCGTGCCGTTGTTGGAAATGCGTCAGCTGGTGCCGATGCTACTTTGATTGATGCCTTGCCGAAGTTGGAGATTGTCTCCAGCTTCAGTGTGGGATTGGATAAGATTGATCTCAAAAAGTGCAAGGAGAAGGGGATTCGGGTCACTAATACTCCCGATGTTTTAACTGAAGACGTTGCGGATTTGGCGATTGGGTTGATTATAGCGGTGCTGAGGAGGTTGTGTGAATGCGATAGATATGTCAGGAGCGGGAAGTGGAAGATTGGTAACTACAAGTTGACTACCAAGTTCTCTGGCAAATCGGTTGGCATTATTGGTTTGGGAAGGATTGGCTTAGCAATTGCCAAGAGAGCTGAAGCATTCAACTGTCCTATATCTTACTACTCAAGAACAAAAAAGGAAGACACCAAGTACAAGTACTACTCAAATCTTCTCGAGTTGGCCTCCAACTCAGATATTCTGATTGTTGCTTGCGCGCTAACTAAAGAAACCCACCACATCGTTAATCGAGAAGTTATTGATGCATTGGGTCCAAAAGGTGTTCTCATCAACATTGGGAGGGGTCCTCACGTCGATGAACCTGAATTAGTAGCTGCCCTGGTTGAAGGCCGATTAGGCGGTGCAGGACTCGATGTGTTTGAAAACGAGCCTGAAGTCCCCCAAGAGCTGTTTGCACTCGAAAATGTTGTCCTGGTGCCGCATATAGGAAGTGGAACTGTTGAAACTCGCAAAGAAATGGCTGACCTTGTGCTTGGAAACCTGGAATCTCACTTCTCCAATAAACCTCTGTTAACTCCAGTGGTATAGTTTTGTGATTGAAACAAATATTAGCCTTCGCTGTTGCCTGGAGAATTCCATGGCATCCTCATCCAGTAACCATCAGACAACACATCGAGTGAACGAATCAATTCGACGAATACTAGCTGGTGATGTTCATAATTTGAATCTTCTATGATTATTGCTTTCATCCCTTACTTGTTGGTATAGTTCAAGTTTTCTTACTCAATCTGATGTAATAGCTTAGCCCTGTTGGTATGATATGTGAAGATATCTTAATAAACGTTGTTGGGGGAAATGCTATCTGTGATATTTGTTTGGTGAAGTCTTCTATTACAATTTGTGTATAATCCAAAACTGAATGGCAAAATTGAAAACTCCTTG

Coding sequence (CDS)

ATGCGCATCGAATCTTCCTCTTCTCCACCTTCATCTTCATCATCTAAATTCAACTACATCTCCTCCGAAAACCCTAATCCAACCCCTAAATTCTCAACCATGGAATCCATCGGTGTACTCATGACTTGTCCGATGAACGCTTACCTTGAGGGGGAGCTGCAGAAGCGCTTCAATCTTTACAAGTTCTGGCAATTTCCTCAGAAGACGCAATTCCTCACGGAGCATTGCAATTCGATTCGTGCCGTTGTTGGAAATGCGTCAGCTGGTGCCGATGCTACTTTGATTGATGCCTTGCCGAAGTTGGAGATTGTCTCCAGCTTCAGTGTGGGATTGGATAAGATTGATCTCAAAAAGTGCAAGGAGAAGGGGATTCGGGTCACTAATACTCCCGATGTTTTAACTGAAGACGTTGCGGATTTGGCGATTGGGTTGATTATAGCGGTGCTGAGGAGGTTGTGTGAATGCGATAGATATGTCAGGAGCGGGAAGTGGAAGATTGGTAACTACAAGTTGACTACCAAGTTCTCTGGCAAATCGGTTGGCATTATTGGTTTGGGAAGGATTGGCTTAGCAATTGCCAAGAGAGCTGAAGCATTCAACTGTCCTATATCTTACTACTCAAGAACAAAAAAGGAAGACACCAAGTACAAGTACTACTCAAATCTTCTCGAGTTGGCCTCCAACTCAGATATTCTGATTGTTGCTTGCGCGCTAACTAAAGAAACCCACCACATCGTTAATCGAGAAGTTATTGATGCATTGGGTCCAAAAGGTGTTCTCATCAACATTGGGAGGGGTCCTCACGTCGATGAACCTGAATTAGTAGCTGCCCTGGTTGAAGGCCGATTAGGCGGTGCAGGACTCGATGTGTTTGAAAACGAGCCTGAAGTCCCCCAAGAGCTGTTTGCACTCGAAAATGTTGTCCTGGTGCCGCATATAGGAAGTGGAACTGTTGAAACTCGCAAAGAAATGGCTGACCTTGTGCTTGGAAACCTGGAATCTCACTTCTCCAATAAACCTCTGTTAACTCCAGTGGTATAG

Protein sequence

MRIESSSSPPSSSSSKFNYISSENPNPTPKFSTMESIGVLMTCPMNAYLEGELQKRFNLYKFWQFPQKTQFLTEHCNSIRAVVGNASAGADATLIDALPKLEIVSSFSVGLDKIDLKKCKEKGIRVTNTPDVLTEDVADLAIGLIIAVLRRLCECDRYVRSGKWKIGNYKLTTKFSGKSVGIIGLGRIGLAIAKRAEAFNCPISYYSRTKKEDTKYKYYSNLLELASNSDILIVACALTKETHHIVNREVIDALGPKGVLINIGRGPHVDEPELVAALVEGRLGGAGLDVFENEPEVPQELFALENVVLVPHIGSGTVETRKEMADLVLGNLESHFSNKPLLTPVV*
Homology
BLAST of CSPI07G21490 vs. ExPASy Swiss-Prot
Match: Q65CJ7 (Hydroxyphenylpyruvate reductase OS=Plectranthus scutellarioides OX=4142 GN=HPPR PE=1 SV=2)

HSP 1 Score: 508.8 bits (1309), Expect = 4.7e-143
Identity = 246/313 (78.59%), Postives = 281/313 (89.78%), Query Frame = 0

Query: 34  MESIGVLMTCPMNAYLEGELQKRFNLYKFWQFPQKTQFLTEHCNSIRAVVGNASAGADAT 93
           ME+IGVLM CPM+ YLE EL KRF L+++W  P +  FL     SIRAVVGN++AGADA 
Sbjct: 1   MEAIGVLMMCPMSTYLEQELDKRFKLFRYWTQPAQRDFLALQAESIRAVVGNSNAGADAE 60

Query: 94  LIDALPKLEIVSSFSVGLDKIDLKKCKEKGIRVTNTPDVLTEDVADLAIGLIIAVLRRLC 153
           LIDALPKLEIVSSFSVGLDK+DL KC+EKG+RVTNTPDVLT+DVADLAIGLI+AVLRR+C
Sbjct: 61  LIDALPKLEIVSSFSVGLDKVDLIKCEEKGVRVTNTPDVLTDDVADLAIGLILAVLRRIC 120

Query: 154 ECDRYVRSGKWKIGNYKLTTKFSGKSVGIIGLGRIGLAIAKRAEAFNCPISYYSRTKKED 213
           ECD+YVR G WK G++KLTTKFSGK VGIIGLGRIGLA+A+RAEAF+CPISY+SR+KK +
Sbjct: 121 ECDKYVRRGAWKFGDFKLTTKFSGKRVGIIGLGRIGLAVAERAEAFDCPISYFSRSKKPN 180

Query: 214 TKYKYYSNLLELASNSDILIVACALTKETHHIVNREVIDALGPKGVLINIGRGPHVDEPE 273
           T Y YY +++ELASNSDIL+VAC LT ET HI+NREVIDALGPKGVLINIGRGPHVDEPE
Sbjct: 181 TNYTYYGSVVELASNSDILVVACPLTPETTHIINREVIDALGPKGVLINIGRGPHVDEPE 240

Query: 274 LVAALVEGRLGGAGLDVFENEPEVPQELFALENVVLVPHIGSGTVETRKEMADLVLGNLE 333
           LV+ALVEGRLGGAGLDVFE EPEVP++LF LENVVL+PH+GSGTVETRK MADLV+GNLE
Sbjct: 241 LVSALVEGRLGGAGLDVFEREPEVPEKLFGLENVVLLPHVGSGTVETRKVMADLVVGNLE 300

Query: 334 SHFSNKPLLTPVV 347
           +HFS KPLLTPVV
Sbjct: 301 AHFSGKPLLTPVV 313

BLAST of CSPI07G21490 vs. ExPASy Swiss-Prot
Match: A5CAL1 (Glyoxylate/hydroxypyruvate/pyruvate reductase 2KGR OS=Vitis vinifera OX=29760 GN=2KGR PE=1 SV=1)

HSP 1 Score: 505.0 bits (1299), Expect = 6.8e-142
Identity = 245/313 (78.27%), Postives = 277/313 (88.50%), Query Frame = 0

Query: 34  MESIGVLMTCPMNAYLEGELQKRFNLYKFWQFPQKTQFLTEHCNSIRAVVGNASAGADAT 93
           MESIGVL+TCPMN YLE EL KRF L++FW FP       EH NSIRAVVGN+  GADA 
Sbjct: 1   MESIGVLLTCPMNPYLEQELDKRFKLFRFWDFPSANDLFREHSNSIRAVVGNSFIGADAQ 60

Query: 94  LIDALPKLEIVSSFSVGLDKIDLKKCKEKGIRVTNTPDVLTEDVADLAIGLIIAVLRRLC 153
           +I+ALPK+EIVSSFSVGLDKIDL +CKEKGIRVTNTPDVLTEDVADLA+ LI+A LRR+C
Sbjct: 61  MIEALPKMEIVSSFSVGLDKIDLVRCKEKGIRVTNTPDVLTEDVADLALALILATLRRIC 120

Query: 154 ECDRYVRSGKWKIGNYKLTTKFSGKSVGIIGLGRIGLAIAKRAEAFNCPISYYSRTKKED 213
           E DRYVRSG WK G++KLTTKF+GKSVGIIGLGRIG AIAKRAE F+CPISY+SRT+K  
Sbjct: 121 ESDRYVRSGSWKKGDFKLTTKFTGKSVGIIGLGRIGSAIAKRAEGFSCPISYHSRTEKPG 180

Query: 214 TKYKYYSNLLELASNSDILIVACALTKETHHIVNREVIDALGPKGVLINIGRGPHVDEPE 273
           T YKYY +++ELASN  IL+VACALT ET HI+NREVI+ALGPKGV+INIGRG HVDEPE
Sbjct: 181 TNYKYYPSVVELASNCQILVVACALTPETRHIINREVINALGPKGVVINIGRGLHVDEPE 240

Query: 274 LVAALVEGRLGGAGLDVFENEPEVPQELFALENVVLVPHIGSGTVETRKEMADLVLGNLE 333
           LV+ALVEGRLGGAGLDVFENEP VP+EL A++NVVL+PH+GSGTVETRK+MADLVLGNLE
Sbjct: 241 LVSALVEGRLGGAGLDVFENEPNVPEELLAMDNVVLLPHVGSGTVETRKDMADLVLGNLE 300

Query: 334 SHFSNKPLLTPVV 347
           +HF NKPLLTPVV
Sbjct: 301 AHFLNKPLLTPVV 313

BLAST of CSPI07G21490 vs. ExPASy Swiss-Prot
Match: Q9CA90 (Glyoxylate/hydroxypyruvate reductase A HPR2 OS=Arabidopsis thaliana OX=3702 GN=HPR2 PE=1 SV=1)

HSP 1 Score: 500.4 bits (1287), Expect = 1.7e-140
Identity = 247/313 (78.91%), Postives = 274/313 (87.54%), Query Frame = 0

Query: 34  MESIGVLMTCPMNAYLEGELQKRFNLYKFWQFPQKTQFLTEHCNSIRAVVGNASAGADAT 93
           MESIGVLM CPM++YLE EL+KRFNL +FW  P+K+  L  H NSIRAVVGNASAGADA 
Sbjct: 1   MESIGVLMMCPMSSYLENELEKRFNLLRFWTSPEKSVLLETHRNSIRAVVGNASAGADAQ 60

Query: 94  LIDALPKLEIVSSFSVGLDKIDLKKCKEKGIRVTNTPDVLTEDVADLAIGLIIAVLRRLC 153
           LI  LP LEIVSSFSVGLDKIDL KCKEKGIRVTNTPDVLTEDVADLAIGLI+A+LRRLC
Sbjct: 61  LISDLPNLEIVSSFSVGLDKIDLGKCKEKGIRVTNTPDVLTEDVADLAIGLILALLRRLC 120

Query: 154 ECDRYVRSGKWKIGNYKLTTKFSGKSVGIIGLGRIGLAIAKRAEAFNCPISYYSRTKKED 213
           ECDRYVRSGKWK G ++LTTKFSGKSVGIIGLGRIG AIAKRAEAF+CPI+YYSRT K D
Sbjct: 121 ECDRYVRSGKWKQGEFQLTTKFSGKSVGIIGLGRIGTAIAKRAEAFSCPINYYSRTIKPD 180

Query: 214 TKYKYYSNLLELASNSDILIVACALTKETHHIVNREVIDALGPKGVLINIGRGPHVDEPE 273
             YKYY  +++LA NSDIL+VAC LT++T HIV+R+V+DALG KGVLINIGRGPHVDE E
Sbjct: 181 VAYKYYPTVVDLAQNSDILVVACPLTEQTRHIVDRQVMDALGAKGVLINIGRGPHVDEQE 240

Query: 274 LVAALVEGRLGGAGLDVFENEPEVPQELFALENVVLVPHIGSGTVETRKEMADLVLGNLE 333
           L+ AL EGRLGGA LDVFE EP VP+ELF LENVVL+PH+GSGTVETR  MADLV+GNLE
Sbjct: 241 LIKALTEGRLGGAALDVFEQEPHVPEELFGLENVVLLPHVGSGTVETRNAMADLVVGNLE 300

Query: 334 SHFSNKPLLTPVV 347
           +HFS K LLTPVV
Sbjct: 301 AHFSGKSLLTPVV 313

BLAST of CSPI07G21490 vs. ExPASy Swiss-Prot
Match: Q9LE33 (Glyoxylate/hydroxypyruvate reductase HPR3 OS=Arabidopsis thaliana OX=3702 GN=HPR3 PE=2 SV=1)

HSP 1 Score: 302.8 bits (774), Expect = 5.2e-81
Identity = 148/276 (53.62%), Postives = 198/276 (71.74%), Query Frame = 0

Query: 71  FLTEHCNSIRAVVGNASAGADATLIDALPKLEIVSSFSVGLDKIDLKKCKEKGIRVTNTP 130
           F   H +S RA V +        L+  LP L+I+   SVG+D IDL  CK +GI +TN  
Sbjct: 45  FFPRHASSARAFVISGRLPVTDELLSHLPSLQILVCTSVGIDHIDLAACKRRGIVITNAG 104

Query: 131 DVLTEDVADLAIGLIIAVLRRLCECDRYVRSGKW-KIGNYKLTTKFSGKSVGIIGLGRIG 190
           +  ++DVAD A+GL+I+VLRR+   DRYVRSG W K G+++L +K SGK VGI+GLG IG
Sbjct: 105 NAFSDDVADCAVGLLISVLRRIPAADRYVRSGNWAKFGDFQLGSKVSGKRVGIVGLGSIG 164

Query: 191 LAIAKRAEAFNCPISYYSRTKKEDTKYKYYSNLLELASNSDILIVACALTKETHHIVNRE 250
             +AKR E+F C ISY SR++K+ + Y+YYS++L LA N+D+L++ C+LT ETHHIVNRE
Sbjct: 165 SFVAKRLESFGCVISYNSRSQKQSSPYRYYSDILSLAENNDVLVLCCSLTDETHHIVNRE 224

Query: 251 VIDALGPKGVLINIGRGPHVDEPELVAALVEGRLGGAGLDVFENEPEVPQELFALENVVL 310
           V++ LG  GV+IN+GRG  +DE E+V  LV+G +GGAGLDVFENEP VPQELF L+NVVL
Sbjct: 225 VMELLGKDGVVINVGRGKLIDEKEMVKCLVDGVIGGAGLDVFENEPAVPQELFGLDNVVL 284

Query: 311 VPHIGSGTVETRKEMADLVLGNLESHFSNKPLLTPV 346
            PH    T  +   +A + L NL++ FSN+PLL+PV
Sbjct: 285 SPHFAVATPGSLDNVAQIALANLKAFFSNRPLLSPV 320

BLAST of CSPI07G21490 vs. ExPASy Swiss-Prot
Match: O58320 (Glyoxylate reductase OS=Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) OX=70601 GN=gyaR PE=1 SV=2)

HSP 1 Score: 228.0 bits (580), Expect = 1.6e-58
Identity = 125/272 (45.96%), Postives = 173/272 (63.60%), Query Frame = 0

Query: 70  QFLTEHCNSIRAVVGNASAGADATLIDALPKLEIVSSFSVGLDKIDLKKCKEKGIRVTNT 129
           + L +    + A+V   S   D  + +  PKL IV++++VG D ID+++  ++GI VTNT
Sbjct: 37  EILLKKVKEVDALVTMLSERIDKEVFENAPKLRIVANYAVGYDNIDIEEATKRGIYVTNT 96

Query: 130 PDVLTEDVADLAIGLIIAVLRRLCECDRYVRSGKWKIGNYKLTTKF------SGKSVGII 189
           PDVLT+  ADLA  L++A  R + + DR+VRSG+WK        K+       GK++GII
Sbjct: 97  PDVLTDATADLAFALLLATARHVVKGDRFVRSGEWKKRGVAWHPKWFLGYDVYGKTIGII 156

Query: 190 GLGRIGLAIAKRAEAFNCPISYYSRTKKEDTKYKY---YSNLLELASNSDILIVACALTK 249
           GLGRIG AIAKRA+ FN  I YYSRT+KE+ + +    +  L +L   SD +++A  LT+
Sbjct: 157 GLGRIGQAIAKRAKGFNMRILYYSRTRKEEVERELNAEFKPLEDLLRESDFVVLAVPLTR 216

Query: 250 ETHHIVNREVIDALGPKGVLINIGRGPHVDEPELVAALVEGRLGGAGLDVFENEPEVPQE 309
           ET+H++N E +  +    +LINI RG  VD   LV AL EG + GAGLDVFE EP   +E
Sbjct: 217 ETYHLINEERLKLMKKTAILINIARGKVVDTNALVKALKEGWIAGAGLDVFEEEPYYNEE 276

Query: 310 LFALENVVLVPHIGSGTVETRKEMADLVLGNL 333
           LF L+NVVL PHIGS +   R+ MA+LV  NL
Sbjct: 277 LFKLDNVVLTPHIGSASFGAREGMAELVAKNL 308

BLAST of CSPI07G21490 vs. ExPASy TrEMBL
Match: A0A0A0KAG9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G446970 PE=3 SV=1)

HSP 1 Score: 684.1 bits (1764), Expect = 3.0e-193
Identity = 346/346 (100.00%), Postives = 346/346 (100.00%), Query Frame = 0

Query: 1   MRIESSSSPPSSSSSKFNYISSENPNPTPKFSTMESIGVLMTCPMNAYLEGELQKRFNLY 60
           MRIESSSSPPSSSSSKFNYISSENPNPTPKFSTMESIGVLMTCPMNAYLEGELQKRFNLY
Sbjct: 1   MRIESSSSPPSSSSSKFNYISSENPNPTPKFSTMESIGVLMTCPMNAYLEGELQKRFNLY 60

Query: 61  KFWQFPQKTQFLTEHCNSIRAVVGNASAGADATLIDALPKLEIVSSFSVGLDKIDLKKCK 120
           KFWQFPQKTQFLTEHCNSIRAVVGNASAGADATLIDALPKLEIVSSFSVGLDKIDLKKCK
Sbjct: 61  KFWQFPQKTQFLTEHCNSIRAVVGNASAGADATLIDALPKLEIVSSFSVGLDKIDLKKCK 120

Query: 121 EKGIRVTNTPDVLTEDVADLAIGLIIAVLRRLCECDRYVRSGKWKIGNYKLTTKFSGKSV 180
           EKGIRVTNTPDVLTEDVADLAIGLIIAVLRRLCECDRYVRSGKWKIGNYKLTTKFSGKSV
Sbjct: 121 EKGIRVTNTPDVLTEDVADLAIGLIIAVLRRLCECDRYVRSGKWKIGNYKLTTKFSGKSV 180

Query: 181 GIIGLGRIGLAIAKRAEAFNCPISYYSRTKKEDTKYKYYSNLLELASNSDILIVACALTK 240
           GIIGLGRIGLAIAKRAEAFNCPISYYSRTKKEDTKYKYYSNLLELASNSDILIVACALTK
Sbjct: 181 GIIGLGRIGLAIAKRAEAFNCPISYYSRTKKEDTKYKYYSNLLELASNSDILIVACALTK 240

Query: 241 ETHHIVNREVIDALGPKGVLINIGRGPHVDEPELVAALVEGRLGGAGLDVFENEPEVPQE 300
           ETHHIVNREVIDALGPKGVLINIGRGPHVDEPELVAALVEGRLGGAGLDVFENEPEVPQE
Sbjct: 241 ETHHIVNREVIDALGPKGVLINIGRGPHVDEPELVAALVEGRLGGAGLDVFENEPEVPQE 300

Query: 301 LFALENVVLVPHIGSGTVETRKEMADLVLGNLESHFSNKPLLTPVV 347
           LFALENVVLVPHIGSGTVETRKEMADLVLGNLESHFSNKPLLTPVV
Sbjct: 301 LFALENVVLVPHIGSGTVETRKEMADLVLGNLESHFSNKPLLTPVV 346

BLAST of CSPI07G21490 vs. ExPASy TrEMBL
Match: A0A5D3BWD3 (Hydroxyphenylpyruvate reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G002200 PE=3 SV=1)

HSP 1 Score: 612.8 bits (1579), Expect = 8.6e-172
Identity = 305/313 (97.44%), Postives = 310/313 (99.04%), Query Frame = 0

Query: 34  MESIGVLMTCPMNAYLEGELQKRFNLYKFWQFPQKTQFLTEHCNSIRAVVGNASAGADAT 93
           MESIGVLMTCPMNAYLE EL KRFNLYKFWQFPQ+TQFLTEHCNSIRAVVGNASAGADAT
Sbjct: 1   MESIGVLMTCPMNAYLEQELDKRFNLYKFWQFPQRTQFLTEHCNSIRAVVGNASAGADAT 60

Query: 94  LIDALPKLEIVSSFSVGLDKIDLKKCKEKGIRVTNTPDVLTEDVADLAIGLIIAVLRRLC 153
           LIDALPKLEIVSSFSVGLDKIDLKKCKEKGIRVTNTPDVLTEDVADLAIGLIIAVLRRLC
Sbjct: 61  LIDALPKLEIVSSFSVGLDKIDLKKCKEKGIRVTNTPDVLTEDVADLAIGLIIAVLRRLC 120

Query: 154 ECDRYVRSGKWKIGNYKLTTKFSGKSVGIIGLGRIGLAIAKRAEAFNCPISYYSRTKKED 213
           ECDRY+RSGKWKIGNYKLTTKFSGKSVGIIGLGRIGLAIAKRAEAFNCPISY+SRTKKED
Sbjct: 121 ECDRYIRSGKWKIGNYKLTTKFSGKSVGIIGLGRIGLAIAKRAEAFNCPISYHSRTKKED 180

Query: 214 TKYKYYSNLLELASNSDILIVACALTKETHHIVNREVIDALGPKGVLINIGRGPHVDEPE 273
           +KYKYYSNLLELASNSDILI+ACALTKETHHIVNREVIDALGPKGVLINIGRG HVDEPE
Sbjct: 181 SKYKYYSNLLELASNSDILIIACALTKETHHIVNREVIDALGPKGVLINIGRGSHVDEPE 240

Query: 274 LVAALVEGRLGGAGLDVFENEPEVPQELFALENVVLVPHIGSGTVETRKEMADLVLGNLE 333
           LVAALVEGRLGGAGLDVFENEPEVPQELFALENVVLVPHIGSGTVETRKEMADLVLGNLE
Sbjct: 241 LVAALVEGRLGGAGLDVFENEPEVPQELFALENVVLVPHIGSGTVETRKEMADLVLGNLE 300

Query: 334 SHFSNKPLLTPVV 347
           SHFSNKPLLTPVV
Sbjct: 301 SHFSNKPLLTPVV 313

BLAST of CSPI07G21490 vs. ExPASy TrEMBL
Match: A0A6J1GP11 (hydroxyphenylpyruvate reductase OS=Cucurbita moschata OX=3662 GN=LOC111456204 PE=3 SV=1)

HSP 1 Score: 598.2 bits (1541), Expect = 2.2e-167
Identity = 295/313 (94.25%), Postives = 308/313 (98.40%), Query Frame = 0

Query: 34  MESIGVLMTCPMNAYLEGELQKRFNLYKFWQFPQKTQFLTEHCNSIRAVVGNASAGADAT 93
           MESIGVLMTCPMNAYLE EL+KRFNLYKFWQFPQ+TQFLTE+CNSIRAVVGNA AGADAT
Sbjct: 1   MESIGVLMTCPMNAYLEKELEKRFNLYKFWQFPQRTQFLTENCNSIRAVVGNAKAGADAT 60

Query: 94  LIDALPKLEIVSSFSVGLDKIDLKKCKEKGIRVTNTPDVLTEDVADLAIGLIIAVLRRLC 153
           LIDALPKLEIVSSFSVGLDKIDLK+CKEKGIRVTNTPDVLTEDVADLAIGLIIAVLRRLC
Sbjct: 61  LIDALPKLEIVSSFSVGLDKIDLKRCKEKGIRVTNTPDVLTEDVADLAIGLIIAVLRRLC 120

Query: 154 ECDRYVRSGKWKIGNYKLTTKFSGKSVGIIGLGRIGLAIAKRAEAFNCPISYYSRTKKED 213
           ECDRYVRSGKWKIG+YKLTTKFSGKSVGI+GLGRIGLAIAKRAEAFNCPISYYSRT+KE 
Sbjct: 121 ECDRYVRSGKWKIGDYKLTTKFSGKSVGILGLGRIGLAIAKRAEAFNCPISYYSRTEKEG 180

Query: 214 TKYKYYSNLLELASNSDILIVACALTKETHHIVNREVIDALGPKGVLINIGRGPHVDEPE 273
           +KYKYYSNLL+LASNSDIL+VACALTKETHHI+NREVIDALGPKGVLINIGRGPHVDEPE
Sbjct: 181 SKYKYYSNLLDLASNSDILVVACALTKETHHIINREVIDALGPKGVLINIGRGPHVDEPE 240

Query: 274 LVAALVEGRLGGAGLDVFENEPEVPQELFALENVVLVPHIGSGTVETRKEMADLVLGNLE 333
           LVAALVEGRLGGAGLDVFENEPEVPQELF LENVVLVPHIGSGTVETR EMA+LVLGNLE
Sbjct: 241 LVAALVEGRLGGAGLDVFENEPEVPQELFTLENVVLVPHIGSGTVETRTEMANLVLGNLE 300

Query: 334 SHFSNKPLLTPVV 347
           +HFSNKPLLTPVV
Sbjct: 301 AHFSNKPLLTPVV 313

BLAST of CSPI07G21490 vs. ExPASy TrEMBL
Match: A0A5D3BXX6 (Hydroxyphenylpyruvate reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G002190 PE=3 SV=1)

HSP 1 Score: 595.1 bits (1533), Expect = 1.9e-166
Identity = 295/313 (94.25%), Postives = 304/313 (97.12%), Query Frame = 0

Query: 34  MESIGVLMTCPMNAYLEGELQKRFNLYKFWQFPQKTQFLTEHCNSIRAVVGNASAGADAT 93
           MESIGVLMTCPMNAYLE EL KRFNLYKFWQFPQ+TQFLTEHCNSIRAVVGNAS GADAT
Sbjct: 1   MESIGVLMTCPMNAYLEQELDKRFNLYKFWQFPQRTQFLTEHCNSIRAVVGNASVGADAT 60

Query: 94  LIDALPKLEIVSSFSVGLDKIDLKKCKEKGIRVTNTPDVLTEDVADLAIGLIIAVLRRLC 153
           LIDALPKLEIVSSFSVGLDKIDLKKCKEKGIRVTNTPDVLTEDVADLAIGLIIAVLRRLC
Sbjct: 61  LIDALPKLEIVSSFSVGLDKIDLKKCKEKGIRVTNTPDVLTEDVADLAIGLIIAVLRRLC 120

Query: 154 ECDRYVRSGKWKIGNYKLTTKFSGKSVGIIGLGRIGLAIAKRAEAFNCPISYYSRTKKED 213
           ECDRY+RSGKWKIGNYKLTTKFSGKSVGIIGLGRIGLAIAKRAEAFNC ISYYSRTKKED
Sbjct: 121 ECDRYIRSGKWKIGNYKLTTKFSGKSVGIIGLGRIGLAIAKRAEAFNCRISYYSRTKKED 180

Query: 214 TKYKYYSNLLELASNSDILIVACALTKETHHIVNREVIDALGPKGVLINIGRGPHVDEPE 273
           +KYKYYSNLLELASNSDILI+ACALTK+T HIVNREVIDALGPKGVLINIGRG HVDEPE
Sbjct: 181 SKYKYYSNLLELASNSDILIIACALTKQTRHIVNREVIDALGPKGVLINIGRGSHVDEPE 240

Query: 274 LVAALVEGRLGGAGLDVFENEPEVPQELFALENVVLVPHIGSGTVETRKEMADLVLGNLE 333
           LV+ALVEGRLGGAG+DVFENEPEVPQELFALENVVLVPHIG+GTVET KEM  LV+GNLE
Sbjct: 241 LVSALVEGRLGGAGIDVFENEPEVPQELFALENVVLVPHIGNGTVETHKEMVGLVVGNLE 300

Query: 334 SHFSNKPLLTPVV 347
           SHFSNKPLLTPVV
Sbjct: 301 SHFSNKPLLTPVV 313

BLAST of CSPI07G21490 vs. ExPASy TrEMBL
Match: A0A6J1JTA0 (hydroxyphenylpyruvate reductase OS=Cucurbita maxima OX=3661 GN=LOC111488676 PE=3 SV=1)

HSP 1 Score: 594.7 bits (1532), Expect = 2.4e-166
Identity = 293/313 (93.61%), Postives = 307/313 (98.08%), Query Frame = 0

Query: 34  MESIGVLMTCPMNAYLEGELQKRFNLYKFWQFPQKTQFLTEHCNSIRAVVGNASAGADAT 93
           MESIGVLMTCPMNAYLE EL+KRFNLYKFWQFPQ+TQFLTE+CNSIRAVVGNA AGADA 
Sbjct: 1   MESIGVLMTCPMNAYLEKELEKRFNLYKFWQFPQRTQFLTENCNSIRAVVGNAKAGADAP 60

Query: 94  LIDALPKLEIVSSFSVGLDKIDLKKCKEKGIRVTNTPDVLTEDVADLAIGLIIAVLRRLC 153
           LIDALPKLEIVSSFSVGLDKIDLK+CKEKGIRVTNTPDVLTEDVADLAIGLIIAVLRRLC
Sbjct: 61  LIDALPKLEIVSSFSVGLDKIDLKRCKEKGIRVTNTPDVLTEDVADLAIGLIIAVLRRLC 120

Query: 154 ECDRYVRSGKWKIGNYKLTTKFSGKSVGIIGLGRIGLAIAKRAEAFNCPISYYSRTKKED 213
           ECDRYVRSGKWKIG+YKLTTKFSGKSVG++GLGRIGLAIAKRAEAFNCPISY+SRT+KE 
Sbjct: 121 ECDRYVRSGKWKIGDYKLTTKFSGKSVGVLGLGRIGLAIAKRAEAFNCPISYHSRTEKEG 180

Query: 214 TKYKYYSNLLELASNSDILIVACALTKETHHIVNREVIDALGPKGVLINIGRGPHVDEPE 273
           +KYKYYSNLLELASNSDIL+VACALTKETHHI+NREVIDALGPKGVLINIGRGPHVDEPE
Sbjct: 181 SKYKYYSNLLELASNSDILVVACALTKETHHIINREVIDALGPKGVLINIGRGPHVDEPE 240

Query: 274 LVAALVEGRLGGAGLDVFENEPEVPQELFALENVVLVPHIGSGTVETRKEMADLVLGNLE 333
           LVAALVEGRLGGAGLDVFENEPEVPQELF LENVVLVPHIGSGTVETR EMA+LVLGNLE
Sbjct: 241 LVAALVEGRLGGAGLDVFENEPEVPQELFTLENVVLVPHIGSGTVETRTEMANLVLGNLE 300

Query: 334 SHFSNKPLLTPVV 347
           +HFSNKPLLTPVV
Sbjct: 301 AHFSNKPLLTPVV 313

BLAST of CSPI07G21490 vs. NCBI nr
Match: XP_004141766.3 (hydroxyphenylpyruvate reductase [Cucumis sativus] >KGN45382.1 hypothetical protein Csa_015936 [Cucumis sativus])

HSP 1 Score: 684.1 bits (1764), Expect = 6.3e-193
Identity = 346/346 (100.00%), Postives = 346/346 (100.00%), Query Frame = 0

Query: 1   MRIESSSSPPSSSSSKFNYISSENPNPTPKFSTMESIGVLMTCPMNAYLEGELQKRFNLY 60
           MRIESSSSPPSSSSSKFNYISSENPNPTPKFSTMESIGVLMTCPMNAYLEGELQKRFNLY
Sbjct: 1   MRIESSSSPPSSSSSKFNYISSENPNPTPKFSTMESIGVLMTCPMNAYLEGELQKRFNLY 60

Query: 61  KFWQFPQKTQFLTEHCNSIRAVVGNASAGADATLIDALPKLEIVSSFSVGLDKIDLKKCK 120
           KFWQFPQKTQFLTEHCNSIRAVVGNASAGADATLIDALPKLEIVSSFSVGLDKIDLKKCK
Sbjct: 61  KFWQFPQKTQFLTEHCNSIRAVVGNASAGADATLIDALPKLEIVSSFSVGLDKIDLKKCK 120

Query: 121 EKGIRVTNTPDVLTEDVADLAIGLIIAVLRRLCECDRYVRSGKWKIGNYKLTTKFSGKSV 180
           EKGIRVTNTPDVLTEDVADLAIGLIIAVLRRLCECDRYVRSGKWKIGNYKLTTKFSGKSV
Sbjct: 121 EKGIRVTNTPDVLTEDVADLAIGLIIAVLRRLCECDRYVRSGKWKIGNYKLTTKFSGKSV 180

Query: 181 GIIGLGRIGLAIAKRAEAFNCPISYYSRTKKEDTKYKYYSNLLELASNSDILIVACALTK 240
           GIIGLGRIGLAIAKRAEAFNCPISYYSRTKKEDTKYKYYSNLLELASNSDILIVACALTK
Sbjct: 181 GIIGLGRIGLAIAKRAEAFNCPISYYSRTKKEDTKYKYYSNLLELASNSDILIVACALTK 240

Query: 241 ETHHIVNREVIDALGPKGVLINIGRGPHVDEPELVAALVEGRLGGAGLDVFENEPEVPQE 300
           ETHHIVNREVIDALGPKGVLINIGRGPHVDEPELVAALVEGRLGGAGLDVFENEPEVPQE
Sbjct: 241 ETHHIVNREVIDALGPKGVLINIGRGPHVDEPELVAALVEGRLGGAGLDVFENEPEVPQE 300

Query: 301 LFALENVVLVPHIGSGTVETRKEMADLVLGNLESHFSNKPLLTPVV 347
           LFALENVVLVPHIGSGTVETRKEMADLVLGNLESHFSNKPLLTPVV
Sbjct: 301 LFALENVVLVPHIGSGTVETRKEMADLVLGNLESHFSNKPLLTPVV 346

BLAST of CSPI07G21490 vs. NCBI nr
Match: KAA0059307.1 (hydroxyphenylpyruvate reductase [Cucumis melo var. makuwa] >TYK04021.1 hydroxyphenylpyruvate reductase [Cucumis melo var. makuwa])

HSP 1 Score: 612.8 bits (1579), Expect = 1.8e-171
Identity = 305/313 (97.44%), Postives = 310/313 (99.04%), Query Frame = 0

Query: 34  MESIGVLMTCPMNAYLEGELQKRFNLYKFWQFPQKTQFLTEHCNSIRAVVGNASAGADAT 93
           MESIGVLMTCPMNAYLE EL KRFNLYKFWQFPQ+TQFLTEHCNSIRAVVGNASAGADAT
Sbjct: 1   MESIGVLMTCPMNAYLEQELDKRFNLYKFWQFPQRTQFLTEHCNSIRAVVGNASAGADAT 60

Query: 94  LIDALPKLEIVSSFSVGLDKIDLKKCKEKGIRVTNTPDVLTEDVADLAIGLIIAVLRRLC 153
           LIDALPKLEIVSSFSVGLDKIDLKKCKEKGIRVTNTPDVLTEDVADLAIGLIIAVLRRLC
Sbjct: 61  LIDALPKLEIVSSFSVGLDKIDLKKCKEKGIRVTNTPDVLTEDVADLAIGLIIAVLRRLC 120

Query: 154 ECDRYVRSGKWKIGNYKLTTKFSGKSVGIIGLGRIGLAIAKRAEAFNCPISYYSRTKKED 213
           ECDRY+RSGKWKIGNYKLTTKFSGKSVGIIGLGRIGLAIAKRAEAFNCPISY+SRTKKED
Sbjct: 121 ECDRYIRSGKWKIGNYKLTTKFSGKSVGIIGLGRIGLAIAKRAEAFNCPISYHSRTKKED 180

Query: 214 TKYKYYSNLLELASNSDILIVACALTKETHHIVNREVIDALGPKGVLINIGRGPHVDEPE 273
           +KYKYYSNLLELASNSDILI+ACALTKETHHIVNREVIDALGPKGVLINIGRG HVDEPE
Sbjct: 181 SKYKYYSNLLELASNSDILIIACALTKETHHIVNREVIDALGPKGVLINIGRGSHVDEPE 240

Query: 274 LVAALVEGRLGGAGLDVFENEPEVPQELFALENVVLVPHIGSGTVETRKEMADLVLGNLE 333
           LVAALVEGRLGGAGLDVFENEPEVPQELFALENVVLVPHIGSGTVETRKEMADLVLGNLE
Sbjct: 241 LVAALVEGRLGGAGLDVFENEPEVPQELFALENVVLVPHIGSGTVETRKEMADLVLGNLE 300

Query: 334 SHFSNKPLLTPVV 347
           SHFSNKPLLTPVV
Sbjct: 301 SHFSNKPLLTPVV 313

BLAST of CSPI07G21490 vs. NCBI nr
Match: KAG7014075.1 (Hydroxyphenylpyruvate reductase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 605.9 bits (1561), Expect = 2.2e-169
Identity = 300/322 (93.17%), Postives = 314/322 (97.52%), Query Frame = 0

Query: 25  PNPTPKFSTMESIGVLMTCPMNAYLEGELQKRFNLYKFWQFPQKTQFLTEHCNSIRAVVG 84
           P  +P FSTMESIGVLMTCPMNAYLE EL+KRFNLYKFWQFPQ+TQFLTE+CNSIRAVVG
Sbjct: 28  PFLSPNFSTMESIGVLMTCPMNAYLEKELEKRFNLYKFWQFPQRTQFLTENCNSIRAVVG 87

Query: 85  NASAGADATLIDALPKLEIVSSFSVGLDKIDLKKCKEKGIRVTNTPDVLTEDVADLAIGL 144
           NA AGADATLIDALPKLEIVSSFSVGLDKIDLK+CKEKGIRVTNTPDVLTEDVADLAIGL
Sbjct: 88  NAKAGADATLIDALPKLEIVSSFSVGLDKIDLKRCKEKGIRVTNTPDVLTEDVADLAIGL 147

Query: 145 IIAVLRRLCECDRYVRSGKWKIGNYKLTTKFSGKSVGIIGLGRIGLAIAKRAEAFNCPIS 204
           IIAVLRRLCECDRYVRSGKWKIG+YKLTTKFSGKSVGI+GLGRIGLAIAKRAEAFNCPIS
Sbjct: 148 IIAVLRRLCECDRYVRSGKWKIGDYKLTTKFSGKSVGILGLGRIGLAIAKRAEAFNCPIS 207

Query: 205 YYSRTKKEDTKYKYYSNLLELASNSDILIVACALTKETHHIVNREVIDALGPKGVLINIG 264
           YYSRT+KE +KYKYYSNLL+LASNSDIL+VACALTKETHHI+NREVIDALGPKGVLINIG
Sbjct: 208 YYSRTEKEGSKYKYYSNLLDLASNSDILVVACALTKETHHIINREVIDALGPKGVLINIG 267

Query: 265 RGPHVDEPELVAALVEGRLGGAGLDVFENEPEVPQELFALENVVLVPHIGSGTVETRKEM 324
           RGPHVDEPELVAALVEGRLGGAGLDVFENEPEVPQELF LENVVLVPHIGSGTVETR EM
Sbjct: 268 RGPHVDEPELVAALVEGRLGGAGLDVFENEPEVPQELFTLENVVLVPHIGSGTVETRTEM 327

Query: 325 ADLVLGNLESHFSNKPLLTPVV 347
           A+LVLGNLE+HFSNKPLLTPVV
Sbjct: 328 ANLVLGNLEAHFSNKPLLTPVV 349

BLAST of CSPI07G21490 vs. NCBI nr
Match: XP_022953776.1 (hydroxyphenylpyruvate reductase [Cucurbita moschata] >KAG6575531.1 Hydroxyphenylpyruvate reductase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 598.2 bits (1541), Expect = 4.5e-167
Identity = 295/313 (94.25%), Postives = 308/313 (98.40%), Query Frame = 0

Query: 34  MESIGVLMTCPMNAYLEGELQKRFNLYKFWQFPQKTQFLTEHCNSIRAVVGNASAGADAT 93
           MESIGVLMTCPMNAYLE EL+KRFNLYKFWQFPQ+TQFLTE+CNSIRAVVGNA AGADAT
Sbjct: 1   MESIGVLMTCPMNAYLEKELEKRFNLYKFWQFPQRTQFLTENCNSIRAVVGNAKAGADAT 60

Query: 94  LIDALPKLEIVSSFSVGLDKIDLKKCKEKGIRVTNTPDVLTEDVADLAIGLIIAVLRRLC 153
           LIDALPKLEIVSSFSVGLDKIDLK+CKEKGIRVTNTPDVLTEDVADLAIGLIIAVLRRLC
Sbjct: 61  LIDALPKLEIVSSFSVGLDKIDLKRCKEKGIRVTNTPDVLTEDVADLAIGLIIAVLRRLC 120

Query: 154 ECDRYVRSGKWKIGNYKLTTKFSGKSVGIIGLGRIGLAIAKRAEAFNCPISYYSRTKKED 213
           ECDRYVRSGKWKIG+YKLTTKFSGKSVGI+GLGRIGLAIAKRAEAFNCPISYYSRT+KE 
Sbjct: 121 ECDRYVRSGKWKIGDYKLTTKFSGKSVGILGLGRIGLAIAKRAEAFNCPISYYSRTEKEG 180

Query: 214 TKYKYYSNLLELASNSDILIVACALTKETHHIVNREVIDALGPKGVLINIGRGPHVDEPE 273
           +KYKYYSNLL+LASNSDIL+VACALTKETHHI+NREVIDALGPKGVLINIGRGPHVDEPE
Sbjct: 181 SKYKYYSNLLDLASNSDILVVACALTKETHHIINREVIDALGPKGVLINIGRGPHVDEPE 240

Query: 274 LVAALVEGRLGGAGLDVFENEPEVPQELFALENVVLVPHIGSGTVETRKEMADLVLGNLE 333
           LVAALVEGRLGGAGLDVFENEPEVPQELF LENVVLVPHIGSGTVETR EMA+LVLGNLE
Sbjct: 241 LVAALVEGRLGGAGLDVFENEPEVPQELFTLENVVLVPHIGSGTVETRTEMANLVLGNLE 300

Query: 334 SHFSNKPLLTPVV 347
           +HFSNKPLLTPVV
Sbjct: 301 AHFSNKPLLTPVV 313

BLAST of CSPI07G21490 vs. NCBI nr
Match: XP_023548193.1 (hydroxyphenylpyruvate reductase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 596.3 bits (1536), Expect = 1.7e-166
Identity = 295/313 (94.25%), Postives = 307/313 (98.08%), Query Frame = 0

Query: 34  MESIGVLMTCPMNAYLEGELQKRFNLYKFWQFPQKTQFLTEHCNSIRAVVGNASAGADAT 93
           MESIGVLMTCPMNAYLE EL+KRFNLYKFWQFPQ+TQFLTE+CNSIRAVVGNA AGADAT
Sbjct: 1   MESIGVLMTCPMNAYLEKELEKRFNLYKFWQFPQRTQFLTENCNSIRAVVGNAKAGADAT 60

Query: 94  LIDALPKLEIVSSFSVGLDKIDLKKCKEKGIRVTNTPDVLTEDVADLAIGLIIAVLRRLC 153
           LIDALPKLEIVSSFSVGLDKIDLK+CKEKGIRVTNTPDVLTEDVADLAIGLIIAVLRRLC
Sbjct: 61  LIDALPKLEIVSSFSVGLDKIDLKRCKEKGIRVTNTPDVLTEDVADLAIGLIIAVLRRLC 120

Query: 154 ECDRYVRSGKWKIGNYKLTTKFSGKSVGIIGLGRIGLAIAKRAEAFNCPISYYSRTKKED 213
           ECDRYVRSGKWKIG+YKLTTKFSGKSVGI+GLGRIGLAIAKRAEAFNCPISYYSRT+KE 
Sbjct: 121 ECDRYVRSGKWKIGDYKLTTKFSGKSVGILGLGRIGLAIAKRAEAFNCPISYYSRTEKEG 180

Query: 214 TKYKYYSNLLELASNSDILIVACALTKETHHIVNREVIDALGPKGVLINIGRGPHVDEPE 273
           +KYKYYSNLLELASNSDIL+VACALTKETHHI+NREVIDALGPKGVLINIGRG HVDEPE
Sbjct: 181 SKYKYYSNLLELASNSDILVVACALTKETHHIINREVIDALGPKGVLINIGRGSHVDEPE 240

Query: 274 LVAALVEGRLGGAGLDVFENEPEVPQELFALENVVLVPHIGSGTVETRKEMADLVLGNLE 333
           LVAALVEGRLGGAGLDVFENEPEVPQELF LENVVLVPHIGSGTVETR EMA+LVLGNLE
Sbjct: 241 LVAALVEGRLGGAGLDVFENEPEVPQELFTLENVVLVPHIGSGTVETRTEMANLVLGNLE 300

Query: 334 SHFSNKPLLTPVV 347
           +HFSNKPLLTPVV
Sbjct: 301 AHFSNKPLLTPVV 313

BLAST of CSPI07G21490 vs. TAIR 10
Match: AT1G79870.1 (D-isomer specific 2-hydroxyacid dehydrogenase family protein )

HSP 1 Score: 500.4 bits (1287), Expect = 1.2e-141
Identity = 247/313 (78.91%), Postives = 274/313 (87.54%), Query Frame = 0

Query: 34  MESIGVLMTCPMNAYLEGELQKRFNLYKFWQFPQKTQFLTEHCNSIRAVVGNASAGADAT 93
           MESIGVLM CPM++YLE EL+KRFNL +FW  P+K+  L  H NSIRAVVGNASAGADA 
Sbjct: 1   MESIGVLMMCPMSSYLENELEKRFNLLRFWTSPEKSVLLETHRNSIRAVVGNASAGADAQ 60

Query: 94  LIDALPKLEIVSSFSVGLDKIDLKKCKEKGIRVTNTPDVLTEDVADLAIGLIIAVLRRLC 153
           LI  LP LEIVSSFSVGLDKIDL KCKEKGIRVTNTPDVLTEDVADLAIGLI+A+LRRLC
Sbjct: 61  LISDLPNLEIVSSFSVGLDKIDLGKCKEKGIRVTNTPDVLTEDVADLAIGLILALLRRLC 120

Query: 154 ECDRYVRSGKWKIGNYKLTTKFSGKSVGIIGLGRIGLAIAKRAEAFNCPISYYSRTKKED 213
           ECDRYVRSGKWK G ++LTTKFSGKSVGIIGLGRIG AIAKRAEAF+CPI+YYSRT K D
Sbjct: 121 ECDRYVRSGKWKQGEFQLTTKFSGKSVGIIGLGRIGTAIAKRAEAFSCPINYYSRTIKPD 180

Query: 214 TKYKYYSNLLELASNSDILIVACALTKETHHIVNREVIDALGPKGVLINIGRGPHVDEPE 273
             YKYY  +++LA NSDIL+VAC LT++T HIV+R+V+DALG KGVLINIGRGPHVDE E
Sbjct: 181 VAYKYYPTVVDLAQNSDILVVACPLTEQTRHIVDRQVMDALGAKGVLINIGRGPHVDEQE 240

Query: 274 LVAALVEGRLGGAGLDVFENEPEVPQELFALENVVLVPHIGSGTVETRKEMADLVLGNLE 333
           L+ AL EGRLGGA LDVFE EP VP+ELF LENVVL+PH+GSGTVETR  MADLV+GNLE
Sbjct: 241 LIKALTEGRLGGAALDVFEQEPHVPEELFGLENVVLLPHVGSGTVETRNAMADLVVGNLE 300

Query: 334 SHFSNKPLLTPVV 347
           +HFS K LLTPVV
Sbjct: 301 AHFSGKSLLTPVV 313

BLAST of CSPI07G21490 vs. TAIR 10
Match: AT1G79870.2 (D-isomer specific 2-hydroxyacid dehydrogenase family protein )

HSP 1 Score: 457.6 bits (1176), Expect = 8.9e-129
Identity = 231/313 (73.80%), Postives = 256/313 (81.79%), Query Frame = 0

Query: 34  MESIGVLMTCPMNAYLEGELQKRFNLYKFWQFPQKTQFLTEHCNSIRAVVGNASAGADAT 93
           MESIGVLM CPM++YLE EL+KRFNL +FW  P+K+  L  H NSIRAVVGNASAGADA 
Sbjct: 1   MESIGVLMMCPMSSYLENELEKRFNLLRFWTSPEKSVLLETHRNSIRAVVGNASAGADAQ 60

Query: 94  LIDALPKLEIVSSFSVGLDKIDLKKCKEKGIRVTNTPDVLTEDVADLAIGLIIAVLRRLC 153
           LI  LP LEIVSSFSVGLDKIDL KCKEKGIRVTNTPDVLTEDVADLAIGLI+A+LRRLC
Sbjct: 61  LISDLPNLEIVSSFSVGLDKIDLGKCKEKGIRVTNTPDVLTEDVADLAIGLILALLRRLC 120

Query: 154 ECDRYVRSGKWKIGNYKLTTKFSGKSVGIIGLGRIGLAIAKRAEAFNCPISYYSRTKKED 213
           ECDRYVRSGKWK                    GRIG AIAKRAEAF+CPI+YYSRT K D
Sbjct: 121 ECDRYVRSGKWK-------------------QGRIGTAIAKRAEAFSCPINYYSRTIKPD 180

Query: 214 TKYKYYSNLLELASNSDILIVACALTKETHHIVNREVIDALGPKGVLINIGRGPHVDEPE 273
             YKYY  +++LA NSDIL+VAC LT++T HIV+R+V+DALG KGVLINIGRGPHVDE E
Sbjct: 181 VAYKYYPTVVDLAQNSDILVVACPLTEQTRHIVDRQVMDALGAKGVLINIGRGPHVDEQE 240

Query: 274 LVAALVEGRLGGAGLDVFENEPEVPQELFALENVVLVPHIGSGTVETRKEMADLVLGNLE 333
           L+ AL EGRLGGA LDVFE EP VP+ELF LENVVL+PH+GSGTVETR  MADLV+GNLE
Sbjct: 241 LIKALTEGRLGGAALDVFEQEPHVPEELFGLENVVLLPHVGSGTVETRNAMADLVVGNLE 294

Query: 334 SHFSNKPLLTPVV 347
           +HFS K LLTPVV
Sbjct: 301 AHFSGKSLLTPVV 294

BLAST of CSPI07G21490 vs. TAIR 10
Match: AT1G12550.1 (D-isomer specific 2-hydroxyacid dehydrogenase family protein )

HSP 1 Score: 302.8 bits (774), Expect = 3.7e-82
Identity = 148/276 (53.62%), Postives = 198/276 (71.74%), Query Frame = 0

Query: 71  FLTEHCNSIRAVVGNASAGADATLIDALPKLEIVSSFSVGLDKIDLKKCKEKGIRVTNTP 130
           F   H +S RA V +        L+  LP L+I+   SVG+D IDL  CK +GI +TN  
Sbjct: 45  FFPRHASSARAFVISGRLPVTDELLSHLPSLQILVCTSVGIDHIDLAACKRRGIVITNAG 104

Query: 131 DVLTEDVADLAIGLIIAVLRRLCECDRYVRSGKW-KIGNYKLTTKFSGKSVGIIGLGRIG 190
           +  ++DVAD A+GL+I+VLRR+   DRYVRSG W K G+++L +K SGK VGI+GLG IG
Sbjct: 105 NAFSDDVADCAVGLLISVLRRIPAADRYVRSGNWAKFGDFQLGSKVSGKRVGIVGLGSIG 164

Query: 191 LAIAKRAEAFNCPISYYSRTKKEDTKYKYYSNLLELASNSDILIVACALTKETHHIVNRE 250
             +AKR E+F C ISY SR++K+ + Y+YYS++L LA N+D+L++ C+LT ETHHIVNRE
Sbjct: 165 SFVAKRLESFGCVISYNSRSQKQSSPYRYYSDILSLAENNDVLVLCCSLTDETHHIVNRE 224

Query: 251 VIDALGPKGVLINIGRGPHVDEPELVAALVEGRLGGAGLDVFENEPEVPQELFALENVVL 310
           V++ LG  GV+IN+GRG  +DE E+V  LV+G +GGAGLDVFENEP VPQELF L+NVVL
Sbjct: 225 VMELLGKDGVVINVGRGKLIDEKEMVKCLVDGVIGGAGLDVFENEPAVPQELFGLDNVVL 284

Query: 311 VPHIGSGTVETRKEMADLVLGNLESHFSNKPLLTPV 346
            PH    T  +   +A + L NL++ FSN+PLL+PV
Sbjct: 285 SPHFAVATPGSLDNVAQIALANLKAFFSNRPLLSPV 320

BLAST of CSPI07G21490 vs. TAIR 10
Match: AT2G45630.2 (D-isomer specific 2-hydroxyacid dehydrogenase family protein )

HSP 1 Score: 270.8 bits (691), Expect = 1.5e-72
Identity = 135/294 (45.92%), Postives = 196/294 (66.67%), Query Frame = 0

Query: 56  RFNLYKFWQFP-QKTQFLTEHCNSIRAVVGNASAGADATLIDALPKLEIVSSFSVGLDKI 115
           +F + K ++ P    +FL  H +SI A++   +A   A LI  LP L +V + S G+D +
Sbjct: 45  KFEILKAFESPLPLPEFLAYHSDSISAIIAPVAAPVTADLIRILPNLRLVVTTSAGVDHV 104

Query: 116 DLKKCKEKGIRVTNTPDVLTEDVADLAIGLIIAVLRRLCECDRYVRSGKWKI-GNYKLTT 175
           DL +C+ +GI V N     +EDVAD A+GL+I V RR+   +R+V+   W + G+Y L +
Sbjct: 105 DLVECRRRGISVANAGSSFSEDVADTAVGLLIDVFRRISAANRFVKQRFWPLKGDYPLGS 164

Query: 176 KFSGKSVGIIGLGRIGLAIAKRAEAFNCPISYYSRTKKE-DTKYKYYSNLLELASNSDIL 235
           K   K +GI+GLG IG  +A R +AF C ISY SR +K  D  Y YY ++ E+A+NSD L
Sbjct: 165 KLGRKRIGIVGLGSIGSKVATRLDAFGCQISYSSRNRKPYDVPYHYYMDIEEMAANSDAL 224

Query: 236 IVACALTKETHHIVNREVIDALGPKGVLINIGRGPHVDEPELVAALVEGRLGGAGLDVFE 295
           I+ C L ++T  ++N++V+ ALG +GV++N+ RG  +DE E+V  L EG +GGAGLDVFE
Sbjct: 225 IICCELNEKTLRLINKDVLSALGKRGVIVNVARGAIIDEEEMVRCLREGEIGGAGLDVFE 284

Query: 296 NEPEVPQELFALENVVLVPHIGSGTVETRKEMADLVLGNLESHFSNKPLLTPVV 347
           +EP VP+ELF L+NVV  PH    T+E  +E+  +V+GN+E+ FSNKPLLTPV+
Sbjct: 285 DEPNVPKELFELDNVVFSPHSAFMTLEGLEELGKVVVGNIEAFFSNKPLLTPVL 338

BLAST of CSPI07G21490 vs. TAIR 10
Match: AT1G68010.1 (hydroxypyruvate reductase )

HSP 1 Score: 143.7 bits (361), Expect = 2.8e-34
Identity = 94/284 (33.10%), Postives = 147/284 (51.76%), Query Frame = 0

Query: 82  VVGNASAGADATLIDALPKL--EIVSSFSVGLDKIDLKKCKEKGIRVTNTPDVLTEDVAD 141
           V+G  +     TL  AL K   +  S+ +VG + +D++   + GI V NTP VLTE  A+
Sbjct: 66  VIGQLTEDWGETLFSALSKAGGKAFSNMAVGYNNVDVEAANKYGIAVGNTPGVLTETTAE 125

Query: 142 LAIGLIIAVLRRLCECDRYVRSGKWK--IGNYKLTTKFSGKSVGIIGLGRIGLAIAK-RA 201
           LA  L +A  RR+ E D ++R G ++  + +  +     G++VG+IG GRIG A A+   
Sbjct: 126 LAASLSLAAARRIVEADEFMRGGLYEGWLPHLFVGNLLKGQTVGVIGAGRIGSAYARMMV 185

Query: 202 EAFNCPISYY-----SRTKKEDTKY--------------KYYSNLLELASNSDILIVACA 261
           E F   + Y+     +R +K  T Y              K  S++ E+   +D++ +   
Sbjct: 186 EGFKMNLIYFDLYQSTRLEKFVTAYGQFLKANGEQPVTWKRASSMEEVLREADLISLHPV 245

Query: 262 LTKETHHIVNREVIDALGPKGVLINIGRGPHVDEPELVAALVEGRLGGAGLDVFENEPEV 321
           L K T+H+VN+E +  +  + +L+N  RGP +DE  LV  L E  +   GLDVFE EP +
Sbjct: 246 LDKTTYHLVNKERLAMMKKEAILVNCSRGPVIDEAALVEHLKENPMFRVGLDVFEEEPFM 305

Query: 322 PQELFALENVVLVPHIGSGTVETRKEMADLVLGNLESHFSNKPL 342
              L   +N ++VPHI S +  TR+ MA L   N+       P+
Sbjct: 306 KPGLADTKNAIVVPHIASASKWTREGMATLAALNVLGRVKGYPI 349

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q65CJ74.7e-14378.59Hydroxyphenylpyruvate reductase OS=Plectranthus scutellarioides OX=4142 GN=HPPR ... [more]
A5CAL16.8e-14278.27Glyoxylate/hydroxypyruvate/pyruvate reductase 2KGR OS=Vitis vinifera OX=29760 GN... [more]
Q9CA901.7e-14078.91Glyoxylate/hydroxypyruvate reductase A HPR2 OS=Arabidopsis thaliana OX=3702 GN=H... [more]
Q9LE335.2e-8153.62Glyoxylate/hydroxypyruvate reductase HPR3 OS=Arabidopsis thaliana OX=3702 GN=HPR... [more]
O583201.6e-5845.96Glyoxylate reductase OS=Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / ... [more]
Match NameE-valueIdentityDescription
A0A0A0KAG93.0e-193100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G446970 PE=3 SV=1[more]
A0A5D3BWD38.6e-17297.44Hydroxyphenylpyruvate reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
A0A6J1GP112.2e-16794.25hydroxyphenylpyruvate reductase OS=Cucurbita moschata OX=3662 GN=LOC111456204 PE... [more]
A0A5D3BXX61.9e-16694.25Hydroxyphenylpyruvate reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
A0A6J1JTA02.4e-16693.61hydroxyphenylpyruvate reductase OS=Cucurbita maxima OX=3661 GN=LOC111488676 PE=3... [more]
Match NameE-valueIdentityDescription
XP_004141766.36.3e-193100.00hydroxyphenylpyruvate reductase [Cucumis sativus] >KGN45382.1 hypothetical prote... [more]
KAA0059307.11.8e-17197.44hydroxyphenylpyruvate reductase [Cucumis melo var. makuwa] >TYK04021.1 hydroxyph... [more]
KAG7014075.12.2e-16993.17Hydroxyphenylpyruvate reductase, partial [Cucurbita argyrosperma subsp. argyrosp... [more]
XP_022953776.14.5e-16794.25hydroxyphenylpyruvate reductase [Cucurbita moschata] >KAG6575531.1 Hydroxyphenyl... [more]
XP_023548193.11.7e-16694.25hydroxyphenylpyruvate reductase [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT1G79870.11.2e-14178.91D-isomer specific 2-hydroxyacid dehydrogenase family protein [more]
AT1G79870.28.9e-12973.80D-isomer specific 2-hydroxyacid dehydrogenase family protein [more]
AT1G12550.13.7e-8253.62D-isomer specific 2-hydroxyacid dehydrogenase family protein [more]
AT2G45630.21.5e-7245.92D-isomer specific 2-hydroxyacid dehydrogenase family protein [more]
AT1G68010.12.8e-3433.10hydroxypyruvate reductase [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006140D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding domainPFAMPF028262-Hacid_dh_Ccoord: 142..314
e-value: 3.7E-54
score: 182.7
IPR006139D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domainPFAMPF003892-Hacid_dhcoord: 44..345
e-value: 7.1E-25
score: 87.5
NoneNo IPR availableGENE3D3.40.50.720coord: 134..317
e-value: 1.8E-133
score: 445.8
NoneNo IPR availableGENE3D3.40.50.720coord: 52..340
e-value: 1.8E-133
score: 445.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..20
NoneNo IPR availablePANTHERPTHR10996:SF235D-GLYCERATE DEHYDROGENASE/HYDROXYPYRUVATE REDUCTASEcoord: 34..346
NoneNo IPR availablePANTHERPTHR109962-HYDROXYACID DEHYDROGENASE-RELATEDcoord: 34..346
NoneNo IPR availableCDDcd12156HPPRcoord: 37..337
e-value: 0.0
score: 517.407
NoneNo IPR availableSUPERFAMILY52283Formate/glycerate dehydrogenase catalytic domain-likecoord: 39..167
IPR029752D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding domain conserved site 1PROSITEPS00065D_2_HYDROXYACID_DH_1coord: 180..207
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 134..315

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI07G21490.1CSPI07G21490.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005829 cytosol
molecular_function GO:0030267 glyoxylate reductase (NADP+) activity
molecular_function GO:0047995 hydroxyphenylpyruvate reductase activity
molecular_function GO:0016618 hydroxypyruvate reductase activity
molecular_function GO:0051287 NAD binding
molecular_function GO:0102742 R(+)-3,4-dihydroxyphenyllactate:NADP+ oxidoreductase activity
molecular_function GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor