CSPI07G21470 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI07G21470
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	glyoxylate/hydroxypyruvate reductase A HPR2-like
Location	Chr7: 18348485 .. 18349265 (-)
RNA-Seq Expression	CSPI07G21470
Synteny	CSPI07G21470

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGACATTCTAATTTTTTTCCAAAATCTAATTTTGGTTATATATATGCGTGTGATCAGTTCAGTGGCAAATCGATAGGAATAATTGGGTTAGGAAGAATCGGGTCAGCCATAGCGAAGAGAGCTCAAGCATTTGGTTGCACCATCAATTATTTCTCAAGGACCGTAAAGCACCATCTCACTGACTACAAATACTTCCCCACTCCTCTAGAGTTGGCTAAGAATTCTCAGATTATGTTTGTTTCATGTGCTCTTACCAAAGAAACCAAGCACATTGTGAACCGAGAAGTACTTGATGCGTTAGGTCCAAATGGCATTCTTATCAACGTCGGTCGAGGTGCTCATGTCGACCAGTCTGAGCTCATCTCGGCATTGCTGGAAGGCCGTTTAGGAGGAGCGGGATTAGATGTATTTGAAAACGAATCCCATGTACCCGAACAATTGATTCACGAGCTTCAGGATAAGACAGTGCTTCTGCCTCATGTGGGTGGGATCTGATACCATTGATACTAATATTGCAATGGCTGACCTTGTCATTGCCAACTTGGAAGCTCATTTCAAGAATCAACCTCTCATCACTCCTGTGAGTTAATTATGTTATATATGATGTTGATTTCTACTTTATTTGTGGTTTTTTTTTACTAAAAGGAAAGTCATTTCTACTATTTACTAAAGTTTTTTTAATTACTATATGGTTTGGATGGAAATATGGTGTATCAATTTTATGAAACTATTAATAAAAAGTTTGGAAAACTTTATGGAAAGTAATGAAATTTTGATA

mRNA sequence

ATGAGACATTCTAATTTTTTTCCAAAATCTAATTTTGGTTATATATATGCGTGTGATCAGTTCAGTGGCAAATCGATAGGAATAATTGGGTTAGGAAGAATCGGGTCAGCCATAGCGAAGAGAGCTCAAGCATTTGGTTGCACCATCAATTATTTCTCAAGGACCGTAAAGCACCATCTCACTGACTACAAATACTTCCCCACTCCTCTAGAGTTGGCTAAGAATTCTCAGATTATGTTTGTTTCATGTGCTCTTACCAAAGAAACCAAGCACATTGTGAACCGAGAAGTACTTGATGCGTTAGGTCCAAATGGCATTCTTATCAACGTCGGTCGAGGTGCTCATGTCGACCAGTCTGAGCTCATCTCGGCATTGCTGGAAGGCCGTTTAGGAGGAGCGGGATTAGATGTATTTGAAAACGAATCCCATGTACCCGAACAATTGATTCACGAGCTTCAGGATAAGACAGTGCTTCTGCCTCATGTGGGTGGGATCTGATACCATTGATACTAATATTGCAATGGCTGACCTTGTCATTGCCAACTTGGAAGCTCATTTCAAGAATCAACCTCTCATCACTCCTGTGAGTTAATTATGTTATATATGATGTTGATTTCTACTTTATTTGTGGTTTTTTTTTACTAAAAGGAAAGTCATTTCTACTATTTACTAAAGTTTTTTTAATTACTATATGGTTTGGATGGAAATATGGTGTATCAATTTTATGAAACTATTAATAAAAAGTTTGGAAAACTTTATGGAAAGTAATGAAATTTTGATA

Coding sequence (CDS)

ATGAGACATTCTAATTTTTTTCCAAAATCTAATTTTGGTTATATATATGCGTGTGATCAGTTCAGTGGCAAATCGATAGGAATAATTGGGTTAGGAAGAATCGGGTCAGCCATAGCGAAGAGAGCTCAAGCATTTGGTTGCACCATCAATTATTTCTCAAGGACCGTAAAGCACCATCTCACTGACTACAAATACTTCCCCACTCCTCTAGAGTTGGCTAAGAATTCTCAGATTATGTTTGTTTCATGTGCTCTTACCAAAGAAACCAAGCACATTGTGAACCGAGAAGTACTTGATGCGTTAGGTCCAAATGGCATTCTTATCAACGTCGGTCGAGGTGCTCATGTCGACCAGTCTGAGCTCATCTCGGCATTGCTGGAAGGCCGTTTAGGAGGAGCGGGATTAGATGTATTTGAAAACGAATCCCATGTACCCGAACAATTGATTCACGAGCTTCAGGATAAGACAGTGCTTCTGCCTCATGTGGGTGGGATCTGA

Protein sequence

MRHSNFFPKSNFGYIYACDQFSGKSIGIIGLGRIGSAIAKRAQAFGCTINYFSRTVKHHLTDYKYFPTPLELAKNSQIMFVSCALTKETKHIVNREVLDALGPNGILINVGRGAHVDQSELISALLEGRLGGAGLDVFENESHVPEQLIHELQDKTVLLPHVGGI*

Homology

[Close]

BLAST of CSPI07G21470 vs. ExPASy Swiss-Prot
Match: A5CAL1 (Glyoxylate/hydroxypyruvate/pyruvate reductase 2KGR OS=Vitis vinifera OX=29760 GN=2KGR PE=1 SV=1)

HSP 1 Score: 201.1 bits (510), Expect = 1.0e-50
Identity = 97/144 (67.36%), Postives = 121/144 (84.03%), Query Frame = 0


Query: 20  QFSGKSIGIIGLGRIGSAIAKRAQAFGCTINYFSRTVKHHLTDYKYFPTPLELAKNSQIM 79
           +F+GKS+GIIGLGRIGSAIAKRA+ F C I+Y SRT K   T+YKY+P+ +ELA N QI+
Sbjct: 141 KFTGKSVGIIGLGRIGSAIAKRAEGFSCPISYHSRTEKPG-TNYKYYPSVVELASNCQIL 200

Query: 80  FVSCALTKETKHIVNREVLDALGPNGILINVGRGAHVDQSELISALLEGRLGGAGLDVFE 139
            V+CALT ET+HI+NREV++ALGP G++IN+GRG HVD+ EL+SAL+EGRLGGAGLDVFE
Sbjct: 201 VVACALTPETRHIINREVINALGPKGVVINIGRGLHVDEPELVSALVEGRLGGAGLDVFE 260

Query: 140 NESHVPEQLIHELQDKTVLLPHVG 164
           NE +VPE+L+    D  VLLPHVG
Sbjct: 261 NEPNVPEELL--AMDNVVLLPHVG 281

[Close]

BLAST of CSPI07G21470 vs. ExPASy Swiss-Prot
Match: Q9CA90 (Glyoxylate/hydroxypyruvate reductase A HPR2 OS=Arabidopsis thaliana OX=3702 GN=HPR2 PE=1 SV=1)

HSP 1 Score: 195.3 bits (495), Expect = 5.5e-49
Identity = 94/144 (65.28%), Postives = 118/144 (81.94%), Query Frame = 0


Query: 20  QFSGKSIGIIGLGRIGSAIAKRAQAFGCTINYFSRTVKHHLTDYKYFPTPLELAKNSQIM 79
           +FSGKS+GIIGLGRIG+AIAKRA+AF C INY+SRT+K  +  YKY+PT ++LA+NS I+
Sbjct: 141 KFSGKSVGIIGLGRIGTAIAKRAEAFSCPINYYSRTIKPDVA-YKYYPTVVDLAQNSDIL 200

Query: 80  FVSCALTKETKHIVNREVLDALGPNGILINVGRGAHVDQSELISALLEGRLGGAGLDVFE 139
            V+C LT++T+HIV+R+V+DALG  G+LIN+GRG HVD+ ELI AL EGRLGGA LDVFE
Sbjct: 201 VVACPLTEQTRHIVDRQVMDALGAKGVLINIGRGPHVDEQELIKALTEGRLGGAALDVFE 260

Query: 140 NESHVPEQLIHELQDKTVLLPHVG 164
            E HVPE+L     +  VLLPHVG
Sbjct: 261 QEPHVPEELFG--LENVVLLPHVG 281

[Close]

BLAST of CSPI07G21470 vs. ExPASy Swiss-Prot
Match: Q65CJ7 (Hydroxyphenylpyruvate reductase OS=Plectranthus scutellarioides OX=4142 GN=HPPR PE=1 SV=2)

HSP 1 Score: 188.7 bits (478), Expect = 5.2e-47
Identity = 94/152 (61.84%), Postives = 117/152 (76.97%), Query Frame = 0


Query: 12  FGYIYACDQFSGKSIGIIGLGRIGSAIAKRAQAFGCTINYFSRTVKHHLTDYKYFPTPLE 71
           FG      +FSGK +GIIGLGRIG A+A+RA+AF C I+YFSR+ K + T+Y Y+ + +E
Sbjct: 133 FGDFKLTTKFSGKRVGIIGLGRIGLAVAERAEAFDCPISYFSRSKKPN-TNYTYYGSVVE 192

Query: 72  LAKNSQIMFVSCALTKETKHIVNREVLDALGPNGILINVGRGAHVDQSELISALLEGRLG 131
           LA NS I+ V+C LT ET HI+NREV+DALGP G+LIN+GRG HVD+ EL+SAL+EGRLG
Sbjct: 193 LASNSDILVVACPLTPETTHIINREVIDALGPKGVLINIGRGPHVDEPELVSALVEGRLG 252

Query: 132 GAGLDVFENESHVPEQLIHELQDKTVLLPHVG 164
           GAGLDVFE E  VPE+L     +  VLLPHVG
Sbjct: 253 GAGLDVFEREPEVPEKLFG--LENVVLLPHVG 281

[Close]

BLAST of CSPI07G21470 vs. ExPASy Swiss-Prot
Match: Q9LE33 (Glyoxylate/hydroxypyruvate reductase HPR3 OS=Arabidopsis thaliana OX=3702 GN=HPR3 PE=2 SV=1)

HSP 1 Score: 152.9 bits (385), Expect = 3.2e-36
Identity = 74/152 (48.68%), Postives = 106/152 (69.74%), Query Frame = 0


Query: 10  SNFGYIYACDQFSGKSIGIIGLGRIGSAIAKRAQAFGCTINYFSRTVKHHLTDYKYFPTP 69
           + FG      + SGK +GI+GLG IGS +AKR ++FGC I+Y SR+ K   + Y+Y+   
Sbjct: 139 AKFGDFQLGSKVSGKRVGIVGLGSIGSFVAKRLESFGCVISYNSRSQKQS-SPYRYYSDI 198

Query: 70  LELAKNSQIMFVSCALTKETKHIVNREVLDALGPNGILINVGRGAHVDQSELISALLEGR 129
           L LA+N+ ++ + C+LT ET HIVNREV++ LG +G++INVGRG  +D+ E++  L++G 
Sbjct: 199 LSLAENNDVLVLCCSLTDETHHIVNREVMELLGKDGVVINVGRGKLIDEKEMVKCLVDGV 258

Query: 130 LGGAGLDVFENESHVPEQLIHELQDKTVLLPH 162
           +GGAGLDVFENE  VP++L     D  VL PH
Sbjct: 259 IGGAGLDVFENEPAVPQELFG--LDNVVLSPH 287

[Close]

BLAST of CSPI07G21470 vs. ExPASy Swiss-Prot
Match: O58320 (Glyoxylate reductase OS=Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) OX=70601 GN=gyaR PE=1 SV=2)

HSP 1 Score: 119.0 bits (297), Expect = 5.0e-26
Identity = 69/147 (46.94%), Postives = 90/147 (61.22%), Query Frame = 0


Query: 23  GKSIGIIGLGRIGSAIAKRAQAFGCTINYFSRTVKHHL-----TDYKYFPTPLE-LAKNS 82
           GK+IGIIGLGRIG AIAKRA+ F   I Y+SRT K  +      ++K    PLE L + S
Sbjct: 150 GKTIGIIGLGRIGQAIAKRAKGFNMRILYYSRTRKEEVERELNAEFK----PLEDLLRES 209

Query: 83  QIMFVSCALTKETKHIVNREVLDALGPNGILINVGRGAHVDQSELISALLEGRLGGAGLD 142
             + ++  LT+ET H++N E L  +    ILIN+ RG  VD + L+ AL EG + GAGLD
Sbjct: 210 DFVVLAVPLTRETYHLINEERLKLMKKTAILINIARGKVVDTNALVKALKEGWIAGAGLD 269

Query: 143 VFENESHVPEQLIHELQDKTVLLPHVG 164
           VFE E +  E+L     D  VL PH+G
Sbjct: 270 VFEEEPYYNEELFK--LDNVVLTPHIG 290

[Close]

BLAST of CSPI07G21470 vs. ExPASy TrEMBL
Match: A0A0A0KC62 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G446960 PE=3 SV=1)

HSP 1 Score: 287.0 bits (733), Expect = 5.2e-74
Identity = 141/144 (97.92%), Postives = 142/144 (98.61%), Query Frame = 0


Query: 20  QFSGKSIGIIGLGRIGSAIAKRAQAFGCTINYFSRTVKHHLTDYKYFPTPLELAKNSQIM 79
           +FSGK IGIIGLGRIGSAIAKRAQAFGCTINYFSRTVKHHLTDYKYFPTPLELAKNSQIM
Sbjct: 140 KFSGKPIGIIGLGRIGSAIAKRAQAFGCTINYFSRTVKHHLTDYKYFPTPLELAKNSQIM 199

Query: 80  FVSCALTKETKHIVNREVLDALGPNGILINVGRGAHVDQSELISALLEGRLGGAGLDVFE 139
           FVSCALTKETKHIVNREVLDALGPNGILINVGRGAHVDQSELISALLEGRLGGAGLDVFE
Sbjct: 200 FVSCALTKETKHIVNREVLDALGPNGILINVGRGAHVDQSELISALLEGRLGGAGLDVFE 259

Query: 140 NESHVPEQLIHELQDKTVLLPHVG 164
           NE HVPEQLIHELQDKTVLLPHVG
Sbjct: 260 NEPHVPEQLIHELQDKTVLLPHVG 283

[Close]

BLAST of CSPI07G21470 vs. ExPASy TrEMBL
Match: A0A5A7UYM8 (Glyoxylate/hydroxypyruvate reductase A HPR2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G002210 PE=3 SV=1)

HSP 1 Score: 252.3 bits (643), Expect = 1.4e-63
Identity = 125/144 (86.81%), Postives = 135/144 (93.75%), Query Frame = 0


Query: 20  QFSGKSIGIIGLGRIGSAIAKRAQAFGCTINYFSRTVKHHLTDYKYFPTPLELAKNSQIM 79
           +FSGKS+GIIGLGRIGSAIAKRAQAFGCTINYFSRT KH  TDYKYFP  L++AKNSQIM
Sbjct: 140 KFSGKSVGIIGLGRIGSAIAKRAQAFGCTINYFSRTEKHQHTDYKYFPKLLDMAKNSQIM 199

Query: 80  FVSCALTKETKHIVNREVLDALGPNGILINVGRGAHVDQSELISALLEGRLGGAGLDVFE 139
           FVSCALT+ETKHIVNREVLDALGPNGILINVGRGAHVD+ ELISALLEGRLGGAGLDV+E
Sbjct: 200 FVSCALTEETKHIVNREVLDALGPNGILINVGRGAHVDEPELISALLEGRLGGAGLDVYE 259

Query: 140 NESHVPEQLIHELQDKTVLLPHVG 164
           NE HV EQLIH+L++ TVLLPHVG
Sbjct: 260 NEPHVAEQLIHQLEN-TVLLPHVG 282

[Close]

BLAST of CSPI07G21470 vs. ExPASy TrEMBL
Match: A0A1S3CG82 (glyoxylate/hydroxypyruvate reductase A HPR2-like OS=Cucumis melo OX=3656 GN=LOC103500586 PE=3 SV=1)

HSP 1 Score: 252.3 bits (643), Expect = 1.4e-63
Identity = 125/144 (86.81%), Postives = 135/144 (93.75%), Query Frame = 0


Query: 20  QFSGKSIGIIGLGRIGSAIAKRAQAFGCTINYFSRTVKHHLTDYKYFPTPLELAKNSQIM 79
           +FSGKS+GIIGLGRIGSAIAKRAQAFGCTINYFSRT KH  TDYKYFP  L++AKNSQIM
Sbjct: 140 KFSGKSVGIIGLGRIGSAIAKRAQAFGCTINYFSRTEKHQHTDYKYFPKLLDMAKNSQIM 199

Query: 80  FVSCALTKETKHIVNREVLDALGPNGILINVGRGAHVDQSELISALLEGRLGGAGLDVFE 139
           FVSCALT+ETKHIVNREVLDALGPNGILINVGRGAHVD+ ELISALLEGRLGGAGLDV+E
Sbjct: 200 FVSCALTEETKHIVNREVLDALGPNGILINVGRGAHVDEPELISALLEGRLGGAGLDVYE 259

Query: 140 NESHVPEQLIHELQDKTVLLPHVG 164
           NE HV EQLIH+L++ TVLLPHVG
Sbjct: 260 NEPHVAEQLIHQLEN-TVLLPHVG 282

[Close]

BLAST of CSPI07G21470 vs. ExPASy TrEMBL
Match: A0A5D3BWA0 (Hydroxyphenylpyruvate reductase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G002220 PE=3 SV=1)

HSP 1 Score: 226.5 bits (576), Expect = 8.3e-56
Identity = 115/160 (71.88%), Postives = 133/160 (83.12%), Query Frame = 0


Query: 4   SNFFPKSNFGYIYACDQFSGKSIGIIGLGRIGSAIAKRAQAFGCTINYFSRTVKHHLTDY 63
           S  + K  FG      +F+GKS+GI+GLGRIGSAIAKRA+AFGC I YFSRT KHH + Y
Sbjct: 128 SGSWMKDEFG---LATRFNGKSVGILGLGRIGSAIAKRAEAFGCNIGYFSRTEKHH-SGY 187

Query: 64  KYFPTPLELAKNSQIMFVSCALTKETKHIVNREVLDALGPNGILINVGRGAHVDQSELIS 123
           KYFP+ L+LA NSQI+FVSC LT+ETKHIVNREV+DALGPNGILINVGRGAHV++SEL+S
Sbjct: 188 KYFPSVLDLAANSQILFVSCTLTEETKHIVNREVIDALGPNGILINVGRGAHVNESELVS 247

Query: 124 ALLEGRLGGAGLDVFENESHVPEQLIHELQDKTVLLPHVG 164
           ALLE RLGGAGLDVFENE HVPE+L+    +  VLLPHVG
Sbjct: 248 ALLERRLGGAGLDVFENEPHVPEKLLE--LNNVVLLPHVG 281

[Close]

BLAST of CSPI07G21470 vs. ExPASy TrEMBL
Match: A0A1S3CG96 (hydroxyphenylpyruvate reductase-like OS=Cucumis melo OX=3656 GN=LOC103500587 PE=3 SV=1)

HSP 1 Score: 226.5 bits (576), Expect = 8.3e-56
Identity = 115/160 (71.88%), Postives = 133/160 (83.12%), Query Frame = 0


Query: 4   SNFFPKSNFGYIYACDQFSGKSIGIIGLGRIGSAIAKRAQAFGCTINYFSRTVKHHLTDY 63
           S  + K  FG      +F+GKS+GI+GLGRIGSAIAKRA+AFGC I YFSRT KHH + Y
Sbjct: 150 SGSWMKDEFG---LATRFNGKSVGILGLGRIGSAIAKRAEAFGCNIGYFSRTEKHH-SGY 209

Query: 64  KYFPTPLELAKNSQIMFVSCALTKETKHIVNREVLDALGPNGILINVGRGAHVDQSELIS 123
           KYFP+ L+LA NSQI+FVSC LT+ETKHIVNREV+DALGPNGILINVGRGAHV++SEL+S
Sbjct: 210 KYFPSVLDLAANSQILFVSCTLTEETKHIVNREVIDALGPNGILINVGRGAHVNESELVS 269

Query: 124 ALLEGRLGGAGLDVFENESHVPEQLIHELQDKTVLLPHVG 164
           ALLE RLGGAGLDVFENE HVPE+L+    +  VLLPHVG
Sbjct: 270 ALLERRLGGAGLDVFENEPHVPEKLLE--LNNVVLLPHVG 303

[Close]

BLAST of CSPI07G21470 vs. NCBI nr
Match: XP_031744698.1 (glyoxylate/hydroxypyruvate reductase A HPR2 isoform X1 [Cucumis sativus])

HSP 1 Score: 287.0 bits (733), Expect = 1.1e-73
Identity = 141/144 (97.92%), Postives = 142/144 (98.61%), Query Frame = 0


Query: 20  QFSGKSIGIIGLGRIGSAIAKRAQAFGCTINYFSRTVKHHLTDYKYFPTPLELAKNSQIM 79
           +FSGK IGIIGLGRIGSAIAKRAQAFGCTINYFSRTVKHHLTDYKYFPTPLELAKNSQIM
Sbjct: 140 KFSGKPIGIIGLGRIGSAIAKRAQAFGCTINYFSRTVKHHLTDYKYFPTPLELAKNSQIM 199

Query: 80  FVSCALTKETKHIVNREVLDALGPNGILINVGRGAHVDQSELISALLEGRLGGAGLDVFE 139
           FVSCALTKETKHIVNREVLDALGPNGILINVGRGAHVDQSELISALLEGRLGGAGLDVFE
Sbjct: 200 FVSCALTKETKHIVNREVLDALGPNGILINVGRGAHVDQSELISALLEGRLGGAGLDVFE 259

Query: 140 NESHVPEQLIHELQDKTVLLPHVG 164
           NE HVPEQLIHELQDKTVLLPHVG
Sbjct: 260 NEPHVPEQLIHELQDKTVLLPHVG 283

[Close]

BLAST of CSPI07G21470 vs. NCBI nr
Match: XP_004141768.1 (glyoxylate/hydroxypyruvate reductase A HPR2 isoform X2 [Cucumis sativus] >KGN45381.1 hypothetical protein Csa_016054 [Cucumis sativus])

HSP 1 Score: 287.0 bits (733), Expect = 1.1e-73
Identity = 141/144 (97.92%), Postives = 142/144 (98.61%), Query Frame = 0


Query: 20  QFSGKSIGIIGLGRIGSAIAKRAQAFGCTINYFSRTVKHHLTDYKYFPTPLELAKNSQIM 79
           +FSGK IGIIGLGRIGSAIAKRAQAFGCTINYFSRTVKHHLTDYKYFPTPLELAKNSQIM
Sbjct: 140 KFSGKPIGIIGLGRIGSAIAKRAQAFGCTINYFSRTVKHHLTDYKYFPTPLELAKNSQIM 199

Query: 80  FVSCALTKETKHIVNREVLDALGPNGILINVGRGAHVDQSELISALLEGRLGGAGLDVFE 139
           FVSCALTKETKHIVNREVLDALGPNGILINVGRGAHVDQSELISALLEGRLGGAGLDVFE
Sbjct: 200 FVSCALTKETKHIVNREVLDALGPNGILINVGRGAHVDQSELISALLEGRLGGAGLDVFE 259

Query: 140 NESHVPEQLIHELQDKTVLLPHVG 164
           NE HVPEQLIHELQDKTVLLPHVG
Sbjct: 260 NEPHVPEQLIHELQDKTVLLPHVG 283

[Close]

BLAST of CSPI07G21470 vs. NCBI nr
Match: KAA0059306.1 (glyoxylate/hydroxypyruvate reductase A HPR2-like [Cucumis melo var. makuwa] >TYK04022.1 glyoxylate/hydroxypyruvate reductase A HPR2-like [Cucumis melo var. makuwa])

HSP 1 Score: 252.3 bits (643), Expect = 2.9e-63
Identity = 125/144 (86.81%), Postives = 135/144 (93.75%), Query Frame = 0


Query: 20  QFSGKSIGIIGLGRIGSAIAKRAQAFGCTINYFSRTVKHHLTDYKYFPTPLELAKNSQIM 79
           +FSGKS+GIIGLGRIGSAIAKRAQAFGCTINYFSRT KH  TDYKYFP  L++AKNSQIM
Sbjct: 140 KFSGKSVGIIGLGRIGSAIAKRAQAFGCTINYFSRTEKHQHTDYKYFPKLLDMAKNSQIM 199

Query: 80  FVSCALTKETKHIVNREVLDALGPNGILINVGRGAHVDQSELISALLEGRLGGAGLDVFE 139
           FVSCALT+ETKHIVNREVLDALGPNGILINVGRGAHVD+ ELISALLEGRLGGAGLDV+E
Sbjct: 200 FVSCALTEETKHIVNREVLDALGPNGILINVGRGAHVDEPELISALLEGRLGGAGLDVYE 259

Query: 140 NESHVPEQLIHELQDKTVLLPHVG 164
           NE HV EQLIH+L++ TVLLPHVG
Sbjct: 260 NEPHVAEQLIHQLEN-TVLLPHVG 282

[Close]

BLAST of CSPI07G21470 vs. NCBI nr
Match: XP_008462161.1 (PREDICTED: glyoxylate/hydroxypyruvate reductase A HPR2-like [Cucumis melo])

HSP 1 Score: 252.3 bits (643), Expect = 2.9e-63
Identity = 125/144 (86.81%), Postives = 135/144 (93.75%), Query Frame = 0


Query: 20  QFSGKSIGIIGLGRIGSAIAKRAQAFGCTINYFSRTVKHHLTDYKYFPTPLELAKNSQIM 79
           +FSGKS+GIIGLGRIGSAIAKRAQAFGCTINYFSRT KH  TDYKYFP  L++AKNSQIM
Sbjct: 140 KFSGKSVGIIGLGRIGSAIAKRAQAFGCTINYFSRTEKHQHTDYKYFPKLLDMAKNSQIM 199

Query: 80  FVSCALTKETKHIVNREVLDALGPNGILINVGRGAHVDQSELISALLEGRLGGAGLDVFE 139
           FVSCALT+ETKHIVNREVLDALGPNGILINVGRGAHVD+ ELISALLEGRLGGAGLDV+E
Sbjct: 200 FVSCALTEETKHIVNREVLDALGPNGILINVGRGAHVDEPELISALLEGRLGGAGLDVYE 259

Query: 140 NESHVPEQLIHELQDKTVLLPHVG 164
           NE HV EQLIH+L++ TVLLPHVG
Sbjct: 260 NEPHVAEQLIHQLEN-TVLLPHVG 282

[Close]

BLAST of CSPI07G21470 vs. NCBI nr
Match: XP_038898271.1 (glyoxylate/hydroxypyruvate reductase A HPR2-like [Benincasa hispida])

HSP 1 Score: 237.7 bits (605), Expect = 7.4e-59
Identity = 120/145 (82.76%), Postives = 134/145 (92.41%), Query Frame = 0


Query: 20  QFSGKSIGIIGLGRIGSAIAKRAQAFGCTINYFSRTVK-HHLTDYKYFPTPLELAKNSQI 79
           +FSGKS+GIIGLGRIGSAIAKRAQAFGC INYFSRT K HH  +YKYFPT L+LA NSQI
Sbjct: 159 RFSGKSVGIIGLGRIGSAIAKRAQAFGCPINYFSRTEKQHHRGNYKYFPTVLDLAANSQI 218

Query: 80  MFVSCALTKETKHIVNREVLDALGPNGILINVGRGAHVDQSELISALLEGRLGGAGLDVF 139
           +FVSCALT++TKHIVNR+V+DALGP GILINVGRGAHVD+SELISALLEGRLGGAGLDVF
Sbjct: 219 LFVSCALTEQTKHIVNRQVIDALGPYGILINVGRGAHVDESELISALLEGRLGGAGLDVF 278

Query: 140 ENESHVPEQLIHELQDKTVLLPHVG 164
           ENE H+P+QL+H LQ+ TVLLPHVG
Sbjct: 279 ENEPHIPQQLLH-LQN-TVLLPHVG 301

[Close]

BLAST of CSPI07G21470 vs. TAIR 10
Match: AT1G79870.1 (D-isomer specific 2-hydroxyacid dehydrogenase family protein )

HSP 1 Score: 195.3 bits (495), Expect = 3.9e-50
Identity = 94/144 (65.28%), Postives = 118/144 (81.94%), Query Frame = 0


Query: 20  QFSGKSIGIIGLGRIGSAIAKRAQAFGCTINYFSRTVKHHLTDYKYFPTPLELAKNSQIM 79
           +FSGKS+GIIGLGRIG+AIAKRA+AF C INY+SRT+K  +  YKY+PT ++LA+NS I+
Sbjct: 141 KFSGKSVGIIGLGRIGTAIAKRAEAFSCPINYYSRTIKPDVA-YKYYPTVVDLAQNSDIL 200

Query: 80  FVSCALTKETKHIVNREVLDALGPNGILINVGRGAHVDQSELISALLEGRLGGAGLDVFE 139
            V+C LT++T+HIV+R+V+DALG  G+LIN+GRG HVD+ ELI AL EGRLGGA LDVFE
Sbjct: 201 VVACPLTEQTRHIVDRQVMDALGAKGVLINIGRGPHVDEQELIKALTEGRLGGAALDVFE 260

Query: 140 NESHVPEQLIHELQDKTVLLPHVG 164
            E HVPE+L     +  VLLPHVG
Sbjct: 261 QEPHVPEELFG--LENVVLLPHVG 281

[Close]

BLAST of CSPI07G21470 vs. TAIR 10
Match: AT1G79870.2 (D-isomer specific 2-hydroxyacid dehydrogenase family protein )

HSP 1 Score: 174.9 bits (442), Expect = 5.5e-44
Identity = 84/132 (63.64%), Postives = 106/132 (80.30%), Query Frame = 0


Query: 32  GRIGSAIAKRAQAFGCTINYFSRTVKHHLTDYKYFPTPLELAKNSQIMFVSCALTKETKH 91
           GRIG+AIAKRA+AF C INY+SRT+K  +  YKY+PT ++LA+NS I+ V+C LT++T+H
Sbjct: 134 GRIGTAIAKRAEAFSCPINYYSRTIKPDVA-YKYYPTVVDLAQNSDILVVACPLTEQTRH 193

Query: 92  IVNREVLDALGPNGILINVGRGAHVDQSELISALLEGRLGGAGLDVFENESHVPEQLIHE 151
           IV+R+V+DALG  G+LIN+GRG HVD+ ELI AL EGRLGGA LDVFE E HVPE+L   
Sbjct: 194 IVDRQVMDALGAKGVLINIGRGPHVDEQELIKALTEGRLGGAALDVFEQEPHVPEELFG- 253

Query: 152 LQDKTVLLPHVG 164
             +  VLLPHVG
Sbjct: 254 -LENVVLLPHVG 262

[Close]

BLAST of CSPI07G21470 vs. TAIR 10
Match: AT1G12550.1 (D-isomer specific 2-hydroxyacid dehydrogenase family protein )

HSP 1 Score: 152.9 bits (385), Expect = 2.2e-37
Identity = 74/152 (48.68%), Postives = 106/152 (69.74%), Query Frame = 0


Query: 10  SNFGYIYACDQFSGKSIGIIGLGRIGSAIAKRAQAFGCTINYFSRTVKHHLTDYKYFPTP 69
           + FG      + SGK +GI+GLG IGS +AKR ++FGC I+Y SR+ K   + Y+Y+   
Sbjct: 139 AKFGDFQLGSKVSGKRVGIVGLGSIGSFVAKRLESFGCVISYNSRSQKQS-SPYRYYSDI 198

Query: 70  LELAKNSQIMFVSCALTKETKHIVNREVLDALGPNGILINVGRGAHVDQSELISALLEGR 129
           L LA+N+ ++ + C+LT ET HIVNREV++ LG +G++INVGRG  +D+ E++  L++G 
Sbjct: 199 LSLAENNDVLVLCCSLTDETHHIVNREVMELLGKDGVVINVGRGKLIDEKEMVKCLVDGV 258

Query: 130 LGGAGLDVFENESHVPEQLIHELQDKTVLLPH 162
           +GGAGLDVFENE  VP++L     D  VL PH
Sbjct: 259 IGGAGLDVFENEPAVPQELFG--LDNVVLSPH 287

[Close]

BLAST of CSPI07G21470 vs. TAIR 10
Match: AT2G45630.2 (D-isomer specific 2-hydroxyacid dehydrogenase family protein )

HSP 1 Score: 135.2 bits (339), Expect = 4.8e-32
Identity = 64/138 (46.38%), Postives = 90/138 (65.22%), Query Frame = 0


Query: 24  KSIGIIGLGRIGSAIAKRAQAFGCTINYFSRTVKHHLTDYKYFPTPLELAKNSQIMFVSC 83
           K IGI+GLG IGS +A R  AFGC I+Y SR  K +   Y Y+    E+A NS  + + C
Sbjct: 169 KRIGIVGLGSIGSKVATRLDAFGCQISYSSRNRKPYDVPYHYYMDIEEMAANSDALIICC 228

Query: 84  ALTKETKHIVNREVLDALGPNGILINVGRGAHVDQSELISALLEGRLGGAGLDVFENESH 143
            L ++T  ++N++VL ALG  G+++NV RGA +D+ E++  L EG +GGAGLDVFE+E +
Sbjct: 229 ELNEKTLRLINKDVLSALGKRGVIVNVARGAIIDEEEMVRCLREGEIGGAGLDVFEDEPN 288

Query: 144 VPEQLIHELQDKTVLLPH 162
           VP++L     D  V  PH
Sbjct: 289 VPKELFE--LDNVVFSPH 304

[Close]

BLAST of CSPI07G21470 vs. TAIR 10
Match: AT1G72190.1 (D-isomer specific 2-hydroxyacid dehydrogenase family protein )

HSP 1 Score: 73.9 bits (180), Expect = 1.3e-13
Identity = 47/155 (30.32%), Postives = 76/155 (49.03%), Query Frame = 0


Query: 19  DQFSGKSIGIIGLGRIGSAIAKRAQAFG----CTINYFSRTV---KHHLTDYKYFPTPL- 78
           D   GK++ I+G G IG  +AKR + FG     T  ++  ++      L D K     + 
Sbjct: 188 DTLLGKTVFILGYGNIGIELAKRLKPFGSRVIATKRFWPASIVDSDSRLVDEKGSHEDIY 247

Query: 79  ELAKNSQIMFVSCALTKETKHIVNREVLDALGPNGILINVGRGAHVDQSELISALLEGRL 138
             A  + I+ V   L KET  IVN+E + ++    +L+N+ RG  ++       L  G L
Sbjct: 248 TFAGKADIVVVCLRLNKETAEIVNKEFICSMKKGALLVNIARGGLINYESAFQNLESGHL 307

Query: 139 GGAGLDVFENESHVPEQLIHELQDKTVLLPHVGGI 166
           GG G+DV  +E   P   I + ++  ++ PHV G+
Sbjct: 308 GGLGIDVAWSEPFDPNDPILKFKN-VIITPHVAGV 341

The following BLAST results are available for this feature:

BLAST of CSPI07G21470 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A5CAL1	1.0e-50	67.36	Glyoxylate/hydroxypyruvate/pyruvate reductase 2KGR OS=Vitis vinifera OX=29760 GN...	[more]
Q9CA90	5.5e-49	65.28	Glyoxylate/hydroxypyruvate reductase A HPR2 OS=Arabidopsis thaliana OX=3702 GN=H...	[more]
Q65CJ7	5.2e-47	61.84	Hydroxyphenylpyruvate reductase OS=Plectranthus scutellarioides OX=4142 GN=HPPR ...	[more]
Q9LE33	3.2e-36	48.68	Glyoxylate/hydroxypyruvate reductase HPR3 OS=Arabidopsis thaliana OX=3702 GN=HPR...	[more]
O58320	5.0e-26	46.94	Glyoxylate reductase OS=Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / ...	[more]

BLAST of CSPI07G21470 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KC62	5.2e-74	97.92	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G446960 PE=3 SV=1	[more]
A0A5A7UYM8	1.4e-63	86.81	Glyoxylate/hydroxypyruvate reductase A HPR2-like OS=Cucumis melo var. makuwa OX=...	[more]
A0A1S3CG82	1.4e-63	86.81	glyoxylate/hydroxypyruvate reductase A HPR2-like OS=Cucumis melo OX=3656 GN=LOC1...	[more]
A0A5D3BWA0	8.3e-56	71.88	Hydroxyphenylpyruvate reductase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E...	[more]
A0A1S3CG96	8.3e-56	71.88	hydroxyphenylpyruvate reductase-like OS=Cucumis melo OX=3656 GN=LOC103500587 PE=...	[more]

BLAST of CSPI07G21470 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_031744698.1	1.1e-73	97.92	glyoxylate/hydroxypyruvate reductase A HPR2 isoform X1 [Cucumis sativus]	[more]
XP_004141768.1	1.1e-73	97.92	glyoxylate/hydroxypyruvate reductase A HPR2 isoform X2 [Cucumis sativus] >KGN453...	[more]
KAA0059306.1	2.9e-63	86.81	glyoxylate/hydroxypyruvate reductase A HPR2-like [Cucumis melo var. makuwa] >TYK...	[more]
XP_008462161.1	2.9e-63	86.81	PREDICTED: glyoxylate/hydroxypyruvate reductase A HPR2-like [Cucumis melo]	[more]
XP_038898271.1	7.4e-59	82.76	glyoxylate/hydroxypyruvate reductase A HPR2-like [Benincasa hispida]	[more]

BLAST of CSPI07G21470 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G79870.1	3.9e-50	65.28	D-isomer specific 2-hydroxyacid dehydrogenase family protein	[more]
AT1G79870.2	5.5e-44	63.64	D-isomer specific 2-hydroxyacid dehydrogenase family protein	[more]
AT1G12550.1	2.2e-37	48.68	D-isomer specific 2-hydroxyacid dehydrogenase family protein	[more]
AT2G45630.2	4.8e-32	46.38	D-isomer specific 2-hydroxyacid dehydrogenase family protein	[more]
AT1G72190.1	1.3e-13	30.32	D-isomer specific 2-hydroxyacid dehydrogenase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.50.720		coord: 17..164 e-value: 9.7E-59 score: 200.5
None	No IPR available	PANTHER	PTHR10996	2-HYDROXYACID DEHYDROGENASE-RELATED	coord: 18..163
None	No IPR available	PANTHER	PTHR10996:SF235	D-GLYCERATE DEHYDROGENASE/HYDROXYPYRUVATE REDUCTASE	coord: 18..163
IPR006140	D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding domain	PFAM	PF02826	2-Hacid_dh_C	coord: 18..163 e-value: 1.8E-41 score: 141.4
IPR029752	D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding domain conserved site 1	PROSITE	PS00065	D_2_HYDROXYACID_DH_1	coord: 26..53
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 16..163

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI07G21470.1	CSPI07G21470.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005829	cytosol
molecular_function	GO:0030267	glyoxylate reductase (NADP+) activity
molecular_function	GO:0016618	hydroxypyruvate reductase activity
molecular_function	GO:0051287	NAD binding