CSPI07G21170 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI07G21170
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	thionin-like protein 2
Location	Chr7: 18133854 .. 18134812 (+)
RNA-Seq Expression	CSPI07G21170
Synteny	CSPI07G21170

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GGATCAAGAGAGAACAAATATATTGTTAAAGAGAAAAGCTTTTGATTTAGAGAATATCCTCCGTCTTCATCAATTCATCGATAATTAATAGAGATGGAGAGCAGCAATGTTATGGCAATCAAATGTGGTTTAATTGTTTGTCTATTCCTAAGCTCGCTCTCCATCGCCCATTCCTTCTTCGACGATAAAACTCAGGTTGAAATATGCTTCAAATCTTGTTTAAACGTATGTCTCTACGATAAGAAAAGCATTCGTTTTTTGTGCCCGTTGAAATGCACCAAAATTTGCGTGGCCGACCCGTGGACCAAAAACTTCCAAGCCCTACTGACCAAAAAGGATTCGTCGTGTGAGCTCGACTGCGCAACTTCTAAGTGTACCAACTCAATTAGCACGAACGATATTATAGGTTTGAAATTTACACTCCTCCACTTTCCCACTTTCTTTTTTTGTTATACTTTATTTGCTAATAAATTTCTTATTTGAAGCACCTATCATACAAAGACTATAATAACAAAACATATATACGGTACCGTGTTTATTAATAATTATTTTCACAAACTAAAATACCTTCTTAGTGAACATTAAGTTGTTGTTGGAAGATTAAGTTTTGTGATTGATACACAATATTTGAAGTTAATTAGCTTTGTTTGTTTTTGTTGATGCAGATGAAAAAGAAATGGGGAAGTGTGTGGATTCTTGCACTCAAAAATGTTCCAACAATGAGAATTGATGATGTGGATCTCCTAAATGAACCTTTACCTCTTTTTTTTTTTCTTTTTCTCCACAAAAAATATAAGTCAGTTTTGTATGAAACAAATTTATTGTTGTATTGTTTCATCATATTATAAATGAAACTCCATTATTTATGTTGGAAGAAAGTTTATGTTTTTTAAATCGATTCACACCCTTTTATAAATTTAATTGATAGAATAAATTCATATTTAAATTGATACAAAAAT

mRNA sequence

GGATCAAGAGAGAACAAATATATTGTTAAAGAGAAAAGCTTTTGATTTAGAGAATATCCTCCGTCTTCATCAATTCATCGATAATTAATAGAGATGGAGAGCAGCAATGTTATGGCAATCAAATGTGGTTTAATTGTTTGTCTATTCCTAAGCTCGCTCTCCATCGCCCATTCCTTCTTCGACGATAAAACTCAGGTTGAAATATGCTTCAAATCTTGTTTAAACGTATGTCTCTACGATAAGAAAAGCATTCGTTTTTTGTGCCCGTTGAAATGCACCAAAATTTGCGTGGCCGACCCGTGGACCAAAAACTTCCAAGCCCTACTGACCAAAAAGGATTCGTCGTGTGAGCTCGACTGCGCAACTTCTAAGTGTACCAACTCAATTAGCACGAACGATATTATAGATGAAAAAGAAATGGGGAAGTGTGTGGATTCTTGCACTCAAAAATGTTCCAACAATGAGAATTGATGATGTGGATCTCCTAAATGAACCTTTACCTCTTTTTTTTTTTCTTTTTCTCCACAAAAAATATAAGTCAGTTTTGTATGAAACAAATTTATTGTTGTATTGTTTCATCATATTATAAATGAAACTCCATTATTTATGTTGGAAGAAAGTTTATGTTTTTTAAATCGATTCACACCCTTTTATAAATTTAATTGATAGAATAAATTCATATTTAAATTGATACAAAAAT

Coding sequence (CDS)

ATGGAGAGCAGCAATGTTATGGCAATCAAATGTGGTTTAATTGTTTGTCTATTCCTAAGCTCGCTCTCCATCGCCCATTCCTTCTTCGACGATAAAACTCAGGTTGAAATATGCTTCAAATCTTGTTTAAACGTATGTCTCTACGATAAGAAAAGCATTCGTTTTTTGTGCCCGTTGAAATGCACCAAAATTTGCGTGGCCGACCCGTGGACCAAAAACTTCCAAGCCCTACTGACCAAAAAGGATTCGTCGTGTGAGCTCGACTGCGCAACTTCTAAGTGTACCAACTCAATTAGCACGAACGATATTATAGATGAAAAAGAAATGGGGAAGTGTGTGGATTCTTGCACTCAAAAATGTTCCAACAATGAGAATTGA

Protein sequence

MESSNVMAIKCGLIVCLFLSSLSIAHSFFDDKTQVEICFKSCLNVCLYDKKSIRFLCPLKCTKICVADPWTKNFQALLTKKDSSCELDCATSKCTNSISTNDIIDEKEMGKCVDSCTQKCSNNEN*

Homology

[Close]

BLAST of CSPI07G21170 vs. ExPASy Swiss-Prot
Match: A8MRP4 (Thionin-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=At1g12663 PE=3 SV=1)

HSP 1 Score: 50.1 bits (118), Expect = 2.2e-05
Identity = 29/86 (33.72%), Postives = 46/86 (53.49%), Query Frame = 0


Query: 38  CFKSCLNVCLYDKKSIRFL-CPLKCTKICVADPWTKNFQA-LLTKKDSSCELDCATSKCT 97
           C+ +CL  C    K  ++L CP  CTK C+  P   +  +  + + D  C+L CAT  C 
Sbjct: 28  CYPACLVECKAGSKFPKYLKCPFTCTKECLQQPSPPSVSSNNIDESDYFCKLGCATYHCV 87

Query: 98  NSISTNDIIDEKEMGKCVDSCTQKCS 122
            S+S+    + + +  CVDSC+ KC+
Sbjct: 88  -SLSSIQNPNVERVSACVDSCSNKCT 112

[Close]

BLAST of CSPI07G21170 vs. ExPASy TrEMBL
Match: A0A0A0KC12 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G440620 PE=4 SV=1)

HSP 1 Score: 258.8 bits (660), Expect = 1.1e-65
Identity = 125/125 (100.00%), Postives = 125/125 (100.00%), Query Frame = 0


Query: 1   MESSNVMAIKCGLIVCLFLSSLSIAHSFFDDKTQVEICFKSCLNVCLYDKKSIRFLCPLK 60
           MESSNVMAIKCGLIVCLFLSSLSIAHSFFDDKTQVEICFKSCLNVCLYDKKSIRFLCPLK
Sbjct: 1   MESSNVMAIKCGLIVCLFLSSLSIAHSFFDDKTQVEICFKSCLNVCLYDKKSIRFLCPLK 60

Query: 61  CTKICVADPWTKNFQALLTKKDSSCELDCATSKCTNSISTNDIIDEKEMGKCVDSCTQKC 120
           CTKICVADPWTKNFQALLTKKDSSCELDCATSKCTNSISTNDIIDEKEMGKCVDSCTQKC
Sbjct: 61  CTKICVADPWTKNFQALLTKKDSSCELDCATSKCTNSISTNDIIDEKEMGKCVDSCTQKC 120

Query: 121 SNNEN 126
           SNNEN
Sbjct: 121 SNNEN 125

[Close]

BLAST of CSPI07G21170 vs. ExPASy TrEMBL
Match: A0A1S3CDU0 (thionin-like protein 2 OS=Cucumis melo OX=3656 GN=LOC103499746 PE=4 SV=1)

HSP 1 Score: 142.1 bits (357), Expect = 1.6e-30
Identity = 75/121 (61.98%), Postives = 86/121 (71.07%), Query Frame = 0


Query: 1   MESSNVMAIKCGLIVCLFLSSLSIAHSFFDDKTQVEICFKSCLNVCLYDKKSIRFLCPLK 60
           MESSNVMA+KCGLIV LFLSSLSIA SF D+ T +  CFKSCL  C+ +KK+ R LCP K
Sbjct: 1   MESSNVMAMKCGLIVWLFLSSLSIADSFIDEPTPLGNCFKSCLAECVVNKKTARLLCPFK 60

Query: 61  CTKICVADPWTKNFQALLTKKDSSCELDCATSKCTNSISTNDIIDEKEMGKCVDSCTQKC 120
           CTKICVADP T +F+ +     SSCEL CA S C    ST +  DEKE+ KCV SC Q C
Sbjct: 61  CTKICVADPSTTHFKFVKVDNYSSCELQCAASMC-GEFSTKENPDEKEVEKCVKSCDQTC 120

Query: 121 S 122
           S
Sbjct: 121 S 120

[Close]

BLAST of CSPI07G21170 vs. ExPASy TrEMBL
Match: A0A0A0K781 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G441625 PE=4 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 1.9e-07
Identity = 41/123 (33.33%), Postives = 64/123 (52.03%), Query Frame = 0


Query: 5   NVMAIKCGLIVCLFLSSLSIAHSFFDDKTQVEICFKSCLNVCLYDKKSIRFLCPLKCTKI 64
           NV+A K  L+  L L+SLS A+S     T  + C+ +C  +C          CPL+C + 
Sbjct: 3   NVLAKKWALMAWLLLTSLSTANS-----TSFQECYATCFVICAITPGVAFSDCPLRCLQA 62

Query: 65  CVADPWTKNFQALLTKKDSS----CELDCATSKCTNSISTNDIIDEKEMGKCVDSCTQKC 124
           C+   +  +   ++ ++       C+L CA S CT   ST     E+++G+CVDSC+Q C
Sbjct: 63  CIVPSFPIDDADVVHRQQQQNHFFCKLGCAASSCT-KFSTKQNPGERKVGRCVDSCSQTC 119

[Close]

BLAST of CSPI07G21170 vs. ExPASy TrEMBL
Match: A0A5N5GA29 (Uncharacterized protein OS=Pyrus ussuriensis x Pyrus communis OX=2448454 GN=D8674_039700 PE=4 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 2.4e-07
Identity = 43/118 (36.44%), Postives = 60/118 (50.85%), Query Frame = 0


Query: 9   IKCGLIVCLFLSSL---SIAHSFFDDKTQVEICFKSCLNVCLYDKKSIRFLCPLKCTKIC 68
           ++  ++VCL L  L     A  FF      ++C+ +C  +C+  K      C   C K C
Sbjct: 6   VRNAVLVCLVLGLLMGHCTAGLFF------KLCYGACFTICMVKKPHNPLTCGFNCLKKC 65

Query: 69  VADPWTKNFQALLTKKDSSCELDCATSKCTNSISTNDIIDEKEMGKCVDSCTQKCSNN 124
            A   T +FQ   T     C+L CATS CT+ IST D  + + +GKCVDSC+  CS N
Sbjct: 66  AAPAQTLDFQ---TNPHHFCKLGCATSLCTH-ISTKDNPNAELVGKCVDSCSGTCSTN 113

[Close]

BLAST of CSPI07G21170 vs. ExPASy TrEMBL
Match: A0A6P8CEJ2 (thionin-like protein 2 OS=Punica granatum OX=22663 GN=LOC116196697 PE=4 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 4.1e-07
Identity = 40/111 (36.04%), Postives = 56/111 (50.45%), Query Frame = 0


Query: 14  IVCLFLSSLSIAHSFFDDKTQVEICFKSCLNVCLYDKKSIRFLCPLKCTKICVADPWTKN 73
           +VC+ L  L  AH           C+KSC  +C+       F C  +C K C+    + +
Sbjct: 15  MVCVVLGLLMGAH---QSMASFPSCYKSCFVLCVIKPGETAFKCAFQCLKDCLVHSASPS 74

Query: 74  -FQALLTKKDSSCELDCATSKCTNSISTNDIIDEKEMGKCVDSCTQKCSNN 124
              AL    D  C+L C+TS CTN +ST     EKE+G CV+SC+  CSN+
Sbjct: 75  PASALSVDTDYFCKLGCSTSLCTN-LSTKTNPGEKEVGGCVESCSGMCSNH 121

[Close]

BLAST of CSPI07G21170 vs. NCBI nr
Match: KGN45341.1 (hypothetical protein Csa_015540 [Cucumis sativus])

HSP 1 Score: 258.8 bits (660), Expect = 2.4e-65
Identity = 125/125 (100.00%), Postives = 125/125 (100.00%), Query Frame = 0


Query: 1   MESSNVMAIKCGLIVCLFLSSLSIAHSFFDDKTQVEICFKSCLNVCLYDKKSIRFLCPLK 60
           MESSNVMAIKCGLIVCLFLSSLSIAHSFFDDKTQVEICFKSCLNVCLYDKKSIRFLCPLK
Sbjct: 1   MESSNVMAIKCGLIVCLFLSSLSIAHSFFDDKTQVEICFKSCLNVCLYDKKSIRFLCPLK 60

Query: 61  CTKICVADPWTKNFQALLTKKDSSCELDCATSKCTNSISTNDIIDEKEMGKCVDSCTQKC 120
           CTKICVADPWTKNFQALLTKKDSSCELDCATSKCTNSISTNDIIDEKEMGKCVDSCTQKC
Sbjct: 61  CTKICVADPWTKNFQALLTKKDSSCELDCATSKCTNSISTNDIIDEKEMGKCVDSCTQKC 120

Query: 121 SNNEN 126
           SNNEN
Sbjct: 121 SNNEN 125

[Close]

BLAST of CSPI07G21170 vs. NCBI nr
Match: XP_008461040.1 (PREDICTED: thionin-like protein 2 [Cucumis melo])

HSP 1 Score: 142.1 bits (357), Expect = 3.2e-30
Identity = 75/121 (61.98%), Postives = 86/121 (71.07%), Query Frame = 0


Query: 1   MESSNVMAIKCGLIVCLFLSSLSIAHSFFDDKTQVEICFKSCLNVCLYDKKSIRFLCPLK 60
           MESSNVMA+KCGLIV LFLSSLSIA SF D+ T +  CFKSCL  C+ +KK+ R LCP K
Sbjct: 1   MESSNVMAMKCGLIVWLFLSSLSIADSFIDEPTPLGNCFKSCLAECVVNKKTARLLCPFK 60

Query: 61  CTKICVADPWTKNFQALLTKKDSSCELDCATSKCTNSISTNDIIDEKEMGKCVDSCTQKC 120
           CTKICVADP T +F+ +     SSCEL CA S C    ST +  DEKE+ KCV SC Q C
Sbjct: 61  CTKICVADPSTTHFKFVKVDNYSSCELQCAASMC-GEFSTKENPDEKEVEKCVKSCDQTC 120

Query: 121 S 122
           S
Sbjct: 121 S 120

[Close]

BLAST of CSPI07G21170 vs. NCBI nr
Match: XP_038896425.1 (thionin-like protein 2 [Benincasa hispida])

HSP 1 Score: 77.0 bits (188), Expect = 1.3e-10
Identity = 52/126 (41.27%), Postives = 64/126 (50.79%), Query Frame = 0


Query: 1   MESSNVMAIKCGLIVCLFLSSLSIAHSFFDDKTQVEICFKSCLNVCLYDKKSIRFLCPLK 60
           MESS  MA+K  L+ CL LSSLSIA+S   +    + C+ SC  VC        F CPL+
Sbjct: 1   MESS--MAMKWALMACLLLSSLSIANSEPANSEPFQECYSSCFGVCSLGPSIYIFDCPLR 60

Query: 61  CTKICVADPWTKN---FQALLTKKDSSCELDCATSKCTNSISTNDIIDEKEMGKCVDSCT 120
           C   C+A P   N    Q         C+L CATS CT    T +    +E+  CVDSC 
Sbjct: 61  CLHSCIALPSNDNAEFHQPQQQHHQFYCKLGCATSTCT-GFGTKEKPAVEEVTSCVDSCA 120

Query: 121 QKCSNN 124
           Q CS N
Sbjct: 121 QVCSMN 123

[Close]

BLAST of CSPI07G21170 vs. NCBI nr
Match: XP_038899383.1 (thionin-like protein 2 [Benincasa hispida])

HSP 1 Score: 65.9 bits (159), Expect = 2.9e-07
Identity = 45/117 (38.46%), Postives = 58/117 (49.57%), Query Frame = 0


Query: 7   MAIKCGLIVCLFLSSLSIAHSFFDDKTQVEICFKSCLNVCLYDKKSIRFLCPLKCTKICV 66
           MA K  LI  L LSSLS A+S     T  + C+ +C  +C          CPL+C + C+
Sbjct: 1   MAKKWALIAWLLLSSLSTANS-----TSFQECYATCFVICAITPGVAFSDCPLRCLQACI 60

Query: 67  --ADPWTKNFQALLTKKDSSCELDCATSKCTNSISTNDIIDEKEMGKCVDSCTQKCS 122
             + P   N      +    C+L CA S CT   ST     EKE+G CVDSC+Q CS
Sbjct: 61  IPSFPIDNNDVHRQEQNQFFCKLGCAVSSCT-KFSTKQNPAEKEVGNCVDSCSQTCS 111

[Close]

BLAST of CSPI07G21170 vs. NCBI nr
Match: XP_011659556.1 (thionin-like protein 2 [Cucumis sativus] >KGN45343.1 hypothetical protein Csa_016713 [Cucumis sativus])

HSP 1 Score: 65.5 bits (158), Expect = 3.8e-07
Identity = 41/123 (33.33%), Postives = 64/123 (52.03%), Query Frame = 0


Query: 5   NVMAIKCGLIVCLFLSSLSIAHSFFDDKTQVEICFKSCLNVCLYDKKSIRFLCPLKCTKI 64
           NV+A K  L+  L L+SLS A+S     T  + C+ +C  +C          CPL+C + 
Sbjct: 3   NVLAKKWALMAWLLLTSLSTANS-----TSFQECYATCFVICAITPGVAFSDCPLRCLQA 62

Query: 65  CVADPWTKNFQALLTKKDSS----CELDCATSKCTNSISTNDIIDEKEMGKCVDSCTQKC 124
           C+   +  +   ++ ++       C+L CA S CT   ST     E+++G+CVDSC+Q C
Sbjct: 63  CIVPSFPIDDADVVHRQQQQNHFFCKLGCAASSCT-KFSTKQNPGERKVGRCVDSCSQTC 119

[Close]

BLAST of CSPI07G21170 vs. TAIR 10
Match: AT1G12663.1 (Predicted to encode a PR (pathogenesis-related) protein. Belongs to the plant thionin (PR-13) family with the following members: At1g66100, At5g36910, At1g72260, At2g15010, At1g12663, At1g12660. )

HSP 1 Score: 50.1 bits (118), Expect = 1.6e-06
Identity = 29/86 (33.72%), Postives = 46/86 (53.49%), Query Frame = 0


Query: 38  CFKSCLNVCLYDKKSIRFL-CPLKCTKICVADPWTKNFQA-LLTKKDSSCELDCATSKCT 97
           C+ +CL  C    K  ++L CP  CTK C+  P   +  +  + + D  C+L CAT  C 
Sbjct: 28  CYPACLVECKAGSKFPKYLKCPFTCTKECLQQPSPPSVSSNNIDESDYFCKLGCATYHCV 87

Query: 98  NSISTNDIIDEKEMGKCVDSCTQKCS 122
            S+S+    + + +  CVDSC+ KC+
Sbjct: 88  -SLSSIQNPNVERVSACVDSCSNKCT 112

[Close]

BLAST of CSPI07G21170 vs. TAIR 10
Match: AT1G12660.1 (Predicted to encode a PR (pathogenesis-related) protein. Belongs to the plant thionin (PR-13) family with the following members: At1g66100, At5g36910, At1g72260, At2g15010, At1g12663, At1g12660. )

HSP 1 Score: 41.6 bits (96), Expect = 5.5e-04
Identity = 27/71 (38.03%), Postives = 37/71 (52.11%), Query Frame = 0


Query: 36  EICFKSCLNVCLYDKKSIRFL-CPLKCTKICVADPWTKNFQALLTKKD---SSCELDCAT 95
           ++C+  CL  C      I+ L CP  C K C   P   +F+A L + D   S CEL CAT
Sbjct: 30  KLCYGGCLVACALIAPPIKKLFCPFLCIKDCKRRPML-SFEANLNEIDQTGSYCELGCAT 89

Query: 96  SKCTNSISTND 103
            +C +S S +D
Sbjct: 90  DRCVSSSSIDD 99

The following BLAST results are available for this feature:

BLAST of CSPI07G21170 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A8MRP4	2.2e-05	33.72	Thionin-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=At1g12663 PE=3 SV=1	[more]

BLAST of CSPI07G21170 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KC12	1.1e-65	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G440620 PE=4 SV=1	[more]
A0A1S3CDU0	1.6e-30	61.98	thionin-like protein 2 OS=Cucumis melo OX=3656 GN=LOC103499746 PE=4 SV=1	[more]
A0A0A0K781	1.9e-07	33.33	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G441625 PE=4 SV=1	[more]
A0A5N5GA29	2.4e-07	36.44	Uncharacterized protein OS=Pyrus ussuriensis x Pyrus communis OX=2448454 GN=D867...	[more]
A0A6P8CEJ2	4.1e-07	36.04	thionin-like protein 2 OS=Punica granatum OX=22663 GN=LOC116196697 PE=4 SV=1	[more]

BLAST of CSPI07G21170 vs. NCBI nr


Match Name	E-value	Identity	Description
KGN45341.1	2.4e-65	100.00	hypothetical protein Csa_015540 [Cucumis sativus]	[more]
XP_008461040.1	3.2e-30	61.98	PREDICTED: thionin-like protein 2 [Cucumis melo]	[more]
XP_038896425.1	1.3e-10	41.27	thionin-like protein 2 [Benincasa hispida]	[more]
XP_038899383.1	2.9e-07	38.46	thionin-like protein 2 [Benincasa hispida]	[more]
XP_011659556.1	3.8e-07	33.33	thionin-like protein 2 [Cucumis sativus] >KGN45343.1 hypothetical protein Csa_01...	[more]

BLAST of CSPI07G21170 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G12663.1	1.6e-06	33.72	Predicted to encode a PR (pathogenesis-related) protein. Belongs to the plant t...	[more]
AT1G12660.1	5.5e-04	38.03	Predicted to encode a PR (pathogenesis-related) protein. Belongs to the plant t...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR038975	Thionin-like protein	PANTHER	PTHR36312	THIONIN-LIKE PROTEIN 1	coord: 9..123
None	No IPR available	PANTHER	PTHR36312:SF1	THIONIN-LIKE PROTEIN 1	coord: 9..123

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI07G21170.1	CSPI07G21170.1	mRNA