CSPI07G21020 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI07G21020
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionLINE-1 retrotransposable element ORF2 protein
LocationChr7: 18006742 .. 18007215 (-)
RNA-Seq ExpressionCSPI07G21020
SyntenyCSPI07G21020
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGAAAATCTCTCCTTTTGGAAAGGAAATTGGTGTGAGATCAGCCCCGTATACACATAAACCATGGTTATTTGCCTTATCTACCATCCAAAATGGCTTCGTAAAAGAAATGTGGAATTGTAGCATTTTGGATTGGGACTTCAAACCAAGAAGATCCCTTCGAAATTGTGAACATCATGCGCAGTGGAATGAAATCAAGCGACATTTCAAAACCCCAAATGACAGTAGAGCTATTGACTATCCCACTTGGAAATTGAACATTAATGGTAAATTTGTCGTTGCTTCTGTTAAGAAAGTCTTTTTTAGCTTGAACCAAAGAGATGATATTGATATTGATGTTAACATCTTTTCTCAACTTTGGAAAACCAAAATCCCCAAGAAATGTCGTTTTTTCATATGGTCTCTTTTACATTGTTGTATTAACACTGCTGACATTCTTCAGAGAAAAGTTTCAACTCTCAATCTGAGTTAA

mRNA sequence

ATGGCGAAAATCTCTCCTTTTGGAAAGGAAATTGGTGTGAGATCAGCCCCGTATACACATAAACCATGGTTATTTGCCTTATCTACCATCCAAAATGGCTTCGTAAAAGAAATGTGGAATTGTAGCATTTTGGATTGGGACTTCAAACCAAGAAGATCCCTTCGAAATTGTGAACATCATGCGCAGTGGAATGAAATCAAGCGACATTTCAAAACCCCAAATGACAGTAGAGCTATTGACTATCCCACTTGGAAATTGAACATTAATGGTAAATTTGTCGTTGCTTCTGTTAAGAAAGTCTTTTTTAGCTTGAACCAAAGAGATGATATTGATATTGATGTTAACATCTTTTCTCAACTTTGGAAAACCAAAATCCCCAAGAAATGTCGTTTTTTCATATGGTCTCTTTTACATTGTTGTATTAACACTGCTGACATTCTTCAGAGAAAAGTTTCAACTCTCAATCTGAGTTAA

Coding sequence (CDS)

ATGGCGAAAATCTCTCCTTTTGGAAAGGAAATTGGTGTGAGATCAGCCCCGTATACACATAAACCATGGTTATTTGCCTTATCTACCATCCAAAATGGCTTCGTAAAAGAAATGTGGAATTGTAGCATTTTGGATTGGGACTTCAAACCAAGAAGATCCCTTCGAAATTGTGAACATCATGCGCAGTGGAATGAAATCAAGCGACATTTCAAAACCCCAAATGACAGTAGAGCTATTGACTATCCCACTTGGAAATTGAACATTAATGGTAAATTTGTCGTTGCTTCTGTTAAGAAAGTCTTTTTTAGCTTGAACCAAAGAGATGATATTGATATTGATGTTAACATCTTTTCTCAACTTTGGAAAACCAAAATCCCCAAGAAATGTCGTTTTTTCATATGGTCTCTTTTACATTGTTGTATTAACACTGCTGACATTCTTCAGAGAAAAGTTTCAACTCTCAATCTGAGTTAA

Protein sequence

MAKISPFGKEIGVRSAPYTHKPWLFALSTIQNGFVKEMWNCSILDWDFKPRRSLRNCEHHAQWNEIKRHFKTPNDSRAIDYPTWKLNINGKFVVASVKKVFFSLNQRDDIDIDVNIFSQLWKTKIPKKCRFFIWSLLHCCINTADILQRKVSTLNLS*
Homology
BLAST of CSPI07G21020 vs. ExPASy TrEMBL
Match: A0A5D3CJ08 (LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold367G00340 PE=4 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 1.3e-26
Identity = 63/135 (46.67%), Postives = 83/135 (61.48%), Query Frame = 0

Query: 20  HKPWLFALSTIQNGFVKEMWNCSILDWDFKPRRSLRNCEHHAQWNEIKRHFKTPNDSRAI 79
           H P LFALST Q+  +K+MWN +++DWD KPRR LR+ E H  W E+K            
Sbjct: 620 HYPRLFALSTNQDNSIKDMWNTTLMDWDLKPRRQLRDWE-HPLWAELKNSLNASFCENGR 679

Query: 80  DYPTWKLNINGKFVVASVKKVFFSLNQRDDIDIDVNIFSQLWKTKIPKKCRFFIWSLLHC 139
           D PTW LN +G + VASVKK     +Q      + N F  LWK+ IPKKC+FFIW+LL+ 
Sbjct: 680 DSPTWILNSDGFYSVASVKKALLQPDQGILALQNQNTFKNLWKSSIPKKCKFFIWTLLYD 739

Query: 140 CINTADILQRKVSTL 155
            +NTAD L +++  L
Sbjct: 740 SVNTADQLTKRMPNL 753

BLAST of CSPI07G21020 vs. ExPASy TrEMBL
Match: A0A5A7TES3 (LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold366G00040 PE=4 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 2.5e-25
Identity = 63/138 (45.65%), Postives = 84/138 (60.87%), Query Frame = 0

Query: 20  HKPWLFALSTIQNGFVKEMWNCSILDWDFKPRRSLRNCEHHAQWNEIKRHFKTPNDSRAI 79
           H P LFALST ++  +++MWN +++DWD  PRR LR  E +  W E+K            
Sbjct: 280 HYPRLFALSTNKDSSIRDMWNNTLMDWDLNPRRQLREWE-YPLWAELKNSLNASFCENGK 339

Query: 80  DYPTWKLNINGKFVVASVKKVFFSLNQRDDIDIDV---NIFSQLWKTKIPKKCRFFIWSL 139
           D PTW LN NG + VASVKK   +L Q D   +D+   N F  LWKT IPKKC FFIW+L
Sbjct: 340 DSPTWTLNSNGLYTVASVKK---ALQQPDQSLLDLQSQNTFKNLWKTSIPKKCIFFIWTL 399

Query: 140 LHCCINTADILQRKVSTL 155
           L+  +NTA+ L +++  L
Sbjct: 400 LYDSVNTAEQLMKRLPNL 413

BLAST of CSPI07G21020 vs. ExPASy TrEMBL
Match: A0A5D3DM72 (LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G002870 PE=4 SV=1)

HSP 1 Score: 121.7 bits (304), Expect = 2.7e-24
Identity = 61/139 (43.88%), Postives = 82/139 (58.99%), Query Frame = 0

Query: 19  THKPWLFALSTIQNGFVKEMWNCSILDWDFKPRRSLRNCEHHAQWNEIKRHFKTPNDSRA 78
           +H P L+ALST +   +++MWN +++DWD  PRR LR  E H  W E+K           
Sbjct: 792 SHYPRLYALSTNKESSIRDMWNNTLMDWDLNPRRQLREWE-HPLWAELKNSLNASFCENG 851

Query: 79  IDYPTWKLNINGKFVVASVKKVFFSLNQRDDIDIDV---NIFSQLWKTKIPKKCRFFIWS 138
            D P W LN NG + VASVKK   +L Q +   +D    N F  LWKT IPKKC FFIW+
Sbjct: 852 NDSPMWTLNSNGLYTVASVKK---TLQQPEQNLLDFQSQNTFKNLWKTSIPKKCIFFIWT 911

Query: 139 LLHCCINTADILQRKVSTL 155
           LL+  +NTA+ L +++  L
Sbjct: 912 LLYDSVNTAEQLMKRLPNL 926

BLAST of CSPI07G21020 vs. ExPASy TrEMBL
Match: A0A1S4E2K5 (LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo OX=3656 GN=LOC107991687 PE=4 SV=1)

HSP 1 Score: 121.7 bits (304), Expect = 2.7e-24
Identity = 61/139 (43.88%), Postives = 82/139 (58.99%), Query Frame = 0

Query: 19  THKPWLFALSTIQNGFVKEMWNCSILDWDFKPRRSLRNCEHHAQWNEIKRHFKTPNDSRA 78
           +H P L+ALST +   +++MWN +++DWD  PRR LR  E H  W E+K           
Sbjct: 792 SHYPRLYALSTNKESSIRDMWNNTLMDWDLNPRRQLREWE-HPLWAELKNSLNASFCENG 851

Query: 79  IDYPTWKLNINGKFVVASVKKVFFSLNQRDDIDIDV---NIFSQLWKTKIPKKCRFFIWS 138
            D P W LN NG + VASVKK   +L Q +   +D    N F  LWKT IPKKC FFIW+
Sbjct: 852 NDSPMWTLNSNGLYTVASVKK---TLQQPEQNLLDFQSQNTFKNLWKTSIPKKCIFFIWT 911

Query: 139 LLHCCINTADILQRKVSTL 155
           LL+  +NTA+ L +++  L
Sbjct: 912 LLYDSVNTAEQLMKRLPNL 926

BLAST of CSPI07G21020 vs. ExPASy TrEMBL
Match: A0A5A7TSA7 (LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G001840 PE=4 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 1.0e-23
Identity = 62/136 (45.59%), Postives = 80/136 (58.82%), Query Frame = 0

Query: 22  PWLFALSTIQNGFVKEMWNCSILDWDFKPRRSLRNCEHHAQWNEIKRHFKTPNDSRAIDY 81
           P LFALST + G VK+ WN S  DW     R LR+ E +  W+ IK    TP  +R +  
Sbjct: 226 PRLFALSTNKKGSVKDFWNPSSNDWHLHINRPLRDHEKNL-WHNIKASLPTPLPNRGLPK 285

Query: 82  PTWKLNINGKFVVASVKKVFFSLNQRDDIDIDVNIFSQLWKTKIPKKCRFFIWSLLHCCI 141
           P WKLN N  F  ASVKK+  S       +   N++  LWK + PKKC+FFIW+L+H CI
Sbjct: 286 PLWKLNSNNIFDTASVKKI-LSEAPVSPANFHPNLYKTLWKVEFPKKCKFFIWTLIHGCI 345

Query: 142 NTADILQRKVSTLNLS 158
           NTAD LQ+++    LS
Sbjct: 346 NTADRLQKRLPNWALS 359

BLAST of CSPI07G21020 vs. NCBI nr
Match: TYK10356.1 (LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa])

HSP 1 Score: 129.4 bits (324), Expect = 2.7e-26
Identity = 63/135 (46.67%), Postives = 83/135 (61.48%), Query Frame = 0

Query: 20  HKPWLFALSTIQNGFVKEMWNCSILDWDFKPRRSLRNCEHHAQWNEIKRHFKTPNDSRAI 79
           H P LFALST Q+  +K+MWN +++DWD KPRR LR+ E H  W E+K            
Sbjct: 620 HYPRLFALSTNQDNSIKDMWNTTLMDWDLKPRRQLRDWE-HPLWAELKNSLNASFCENGR 679

Query: 80  DYPTWKLNINGKFVVASVKKVFFSLNQRDDIDIDVNIFSQLWKTKIPKKCRFFIWSLLHC 139
           D PTW LN +G + VASVKK     +Q      + N F  LWK+ IPKKC+FFIW+LL+ 
Sbjct: 680 DSPTWILNSDGFYSVASVKKALLQPDQGILALQNQNTFKNLWKSSIPKKCKFFIWTLLYD 739

Query: 140 CINTADILQRKVSTL 155
            +NTAD L +++  L
Sbjct: 740 SVNTADQLTKRMPNL 753

BLAST of CSPI07G21020 vs. NCBI nr
Match: XP_031744692.1 (uncharacterized protein LOC116405125 [Cucumis sativus])

HSP 1 Score: 127.5 bits (319), Expect = 1.0e-25
Identity = 65/142 (45.77%), Postives = 85/142 (59.86%), Query Frame = 0

Query: 16  APYTH-KPWLFALSTIQNGFVKEMWNCSILDWDFKPRRSLRNCEHHAQWNEIKRHFKTPN 75
           +P+T   P LFALST +   +  MWN    DWD  PRR LR+ E    W ++K       
Sbjct: 35  SPFTQTNPRLFALSTKKGDSIANMWNAEKADWDLYPRRPLRSVE-EVLWEDMKVSLPPLP 94

Query: 76  DSRAIDYPTWKLNINGKFVVASVKKVFFSLNQRDDIDIDVNIFSQLWKTKIPKKCRFFIW 135
           DS   D P W LN NG F VAS+K      NQ +  + D  I++ LWK+ IPK+C+FF+W
Sbjct: 95  DS-GFDNPRWTLNNNGSFTVASIKLARPLNNQDETNEDDGEIYNNLWKSTIPKRCKFFVW 154

Query: 136 SLLHCCINTADILQRKVSTLNL 157
           +LLH CINT D+LQR++ T NL
Sbjct: 155 TLLHECINTMDMLQRRLPTWNL 174

BLAST of CSPI07G21020 vs. NCBI nr
Match: KAA0040037.1 (LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa])

HSP 1 Score: 125.2 bits (313), Expect = 5.1e-25
Identity = 63/138 (45.65%), Postives = 84/138 (60.87%), Query Frame = 0

Query: 20  HKPWLFALSTIQNGFVKEMWNCSILDWDFKPRRSLRNCEHHAQWNEIKRHFKTPNDSRAI 79
           H P LFALST ++  +++MWN +++DWD  PRR LR  E +  W E+K            
Sbjct: 280 HYPRLFALSTNKDSSIRDMWNNTLMDWDLNPRRQLREWE-YPLWAELKNSLNASFCENGK 339

Query: 80  DYPTWKLNINGKFVVASVKKVFFSLNQRDDIDIDV---NIFSQLWKTKIPKKCRFFIWSL 139
           D PTW LN NG + VASVKK   +L Q D   +D+   N F  LWKT IPKKC FFIW+L
Sbjct: 340 DSPTWTLNSNGLYTVASVKK---ALQQPDQSLLDLQSQNTFKNLWKTSIPKKCIFFIWTL 399

Query: 140 LHCCINTADILQRKVSTL 155
           L+  +NTA+ L +++  L
Sbjct: 400 LYDSVNTAEQLMKRLPNL 413

BLAST of CSPI07G21020 vs. NCBI nr
Match: XP_016902461.1 (PREDICTED: LINE-1 retrotransposable element ORF2 protein [Cucumis melo])

HSP 1 Score: 121.7 bits (304), Expect = 5.7e-24
Identity = 61/139 (43.88%), Postives = 82/139 (58.99%), Query Frame = 0

Query: 19  THKPWLFALSTIQNGFVKEMWNCSILDWDFKPRRSLRNCEHHAQWNEIKRHFKTPNDSRA 78
           +H P L+ALST +   +++MWN +++DWD  PRR LR  E H  W E+K           
Sbjct: 792 SHYPRLYALSTNKESSIRDMWNNTLMDWDLNPRRQLREWE-HPLWAELKNSLNASFCENG 851

Query: 79  IDYPTWKLNINGKFVVASVKKVFFSLNQRDDIDIDV---NIFSQLWKTKIPKKCRFFIWS 138
            D P W LN NG + VASVKK   +L Q +   +D    N F  LWKT IPKKC FFIW+
Sbjct: 852 NDSPMWTLNSNGLYTVASVKK---TLQQPEQNLLDFQSQNTFKNLWKTSIPKKCIFFIWT 911

Query: 139 LLHCCINTADILQRKVSTL 155
           LL+  +NTA+ L +++  L
Sbjct: 912 LLYDSVNTAEQLMKRLPNL 926

BLAST of CSPI07G21020 vs. NCBI nr
Match: KAA0039770.1 (LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa] >TYK24727.1 LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa])

HSP 1 Score: 121.7 bits (304), Expect = 5.7e-24
Identity = 61/139 (43.88%), Postives = 82/139 (58.99%), Query Frame = 0

Query: 19  THKPWLFALSTIQNGFVKEMWNCSILDWDFKPRRSLRNCEHHAQWNEIKRHFKTPNDSRA 78
           +H P L+ALST +   +++MWN +++DWD  PRR LR  E H  W E+K           
Sbjct: 792 SHYPRLYALSTNKESSIRDMWNNTLMDWDLNPRRQLREWE-HPLWAELKNSLNASFCENG 851

Query: 79  IDYPTWKLNINGKFVVASVKKVFFSLNQRDDIDIDV---NIFSQLWKTKIPKKCRFFIWS 138
            D P W LN NG + VASVKK   +L Q +   +D    N F  LWKT IPKKC FFIW+
Sbjct: 852 NDSPMWTLNSNGLYTVASVKK---TLQQPEQNLLDFQSQNTFKNLWKTSIPKKCIFFIWT 911

Query: 139 LLHCCINTADILQRKVSTL 155
           LL+  +NTA+ L +++  L
Sbjct: 912 LLYDSVNTAEQLMKRLPNL 926

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A5D3CJ081.3e-2646.67LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=119... [more]
A0A5A7TES32.5e-2545.65LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=119... [more]
A0A5D3DM722.7e-2443.88LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=119... [more]
A0A1S4E2K52.7e-2443.88LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo OX=3656 GN=LOC1079... [more]
A0A5A7TSA71.0e-2345.59LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=119... [more]
Match NameE-valueIdentityDescription
TYK10356.12.7e-2646.67LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa][more]
XP_031744692.11.0e-2545.77uncharacterized protein LOC116405125 [Cucumis sativus][more]
KAA0040037.15.1e-2545.65LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa][more]
XP_016902461.15.7e-2443.88PREDICTED: LINE-1 retrotransposable element ORF2 protein [Cucumis melo][more]
KAA0039770.15.7e-2443.88LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa] >TYK247... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR026960Reverse transcriptase zinc-binding domainPFAMPF13966zf-RVTcoord: 96..150
e-value: 2.3E-7
score: 31.4

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI07G21020.1CSPI07G21020.1mRNA