CSPI07G21000 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI07G21000
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Location	Chr7: 17996322 .. 17998468 (+)
RNA-Seq Expression	CSPI07G21000
Synteny	CSPI07G21000

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTTCAGAAACGACGGAGGAGATCAGGCTGCCGGTGATAAATTTCAGTGATGAAAAAGTGAAGGCGGAGGGCGGCGTCGAGTGGCGGAGAACGAGCAAAAAAGTCCGGGAAGCCATGGAAGAGTACGGATGTTTTATAGCGAAGTATGAGGGAGTAAGTAAAGAACTTAGGGAGGAAGTGTTGAAGGCAGTGAAAGAACTTTTTGAGCTTCCAATTGAGAGGAAGAAGATGAATAAATATGAAAAGCCTTTTAATGGATACGTTGGAGAGCTTCGTACTCTTCCTCTTCATGAAAGCTTGGGAATTGATGATGCTACTAACTTCAATCAAGTTCAATCCTTCTCTCATCTCATGTGGCCGCCGGAGGGAAATCACCATTTTAGGTCATTTTCTCTTCTTCTTTTACATCTTTCTTTCTTTCAATTTTTCGTTTTTCAGATAGTTAGCAAATTTTACATAAGATGTTCAAATAATCTAAGTCTCATGCTCGTTATGCTAAAAAAAAGTTTACGGAGAAATCTTATGCTTTTTATACGATTTGATGAACTTATTAGTAATCTTATTGTCAATTGGTATATGAACTAATGTTATTTAATATAAGATATTTTAAAAATGATAAAATATTAAAACTAATTACAAAATATAACAAAATTCATCAAACTCTTAAGTACATTTTGTAACTAATTTTAGTATTTTGCTATGGAGAACAATTTTTCTTAATATATGAACCCTAAACCCTTAATATTTTGAGGATGTGAATTGGATCACGTATCATCTGATATGTAGATATTTGTTTTAGCAAGATATAACAAAATTTCACATACCTCAAATAGAAATATGAAATTTTGTTGTACTGTTTTTTTTTATAATTTTTTTTAACACTATTACAACAGAATGTTATTATATATATTTTTTCTGTGGGTGCTGACTTTTATAAATGAGTTGGGATTATTGGATGTTTACTTTGTGGGTTTTTGAGTACTGATCTTGGGAGCTAGCTGCTTTATTATTAGTGGGTTGGTCTCAATAAAGAATTCTTAGCTTCTTGGAGAATGTTGTTCAAATGGATCCAAACAAGAGACAAGACTAGGTAGCCTAGCTAGTTCAATAATAATGAAAGATAAATGAAAGAATTGGACAAAGATAAAACTTCTTATTTGGAGGGGTTTGGATACATAATTGTTTTGGTTGAAGAGAGAAGTGATAATAATGTTCTTAATTTTTGGTTTGTTTTTTCAGTGCAACTTTGCATTCATTTGCAAAGATAGCAGAAGAATTGGACAAAATGGTGACCAAAATGATATTTGAAAGCTATGGTGTGGAAAAGTATTACAATTCTCATGCTGATTCCATTACTTATCTTCTTAGAGTTTTAAAAAGCAAAGCTCCTCAATCTCCAGACCCTACTTTATGTCTTGTGGATCACACTGATAAGAGCTTCACCACCATGGTCTATCAAGATCATATCAATGGTTTGGAGCTCAAAACCAAAAATGGCCAATGGATTCAAGTTGACAACTCTTCACCCTCAAGCTTCCTCGTCATTGCTGGAGACGCAATCAAGGTTGAATATATCATTATACATATCATACAATTTTTGAATTTTAATGACATTGATGAACTCAAAGTTGAAAGACTAAACTTTTAATAATTTAGTAAAATTCATTCCTACATTCTTATATTATTATCATCAAATCGACAAATAAAGAAAATAAAAATTGTAAACAATATAGAATCGATGAAAAAAAGTTAATATAGTGGACTCTAACAAAAGATAAATATATCTATAACGTTAAATAGATATGTAAATTCTCTGCTATTTGTCGATTTCATGATCATTATATTTTGAGAATAAACCCTAATAATTGTATACTGTTTTCTTTTTTTGTTGGGTTGAGAAGGCATGGAGCAATGATAGAATAGAATCACCAACTCATAAAGTGACGATAAGAGGAAATGAAGATAGGTATTCTCTTGCGATGTTTGGATTCAGCAATGGAATTGTGGAAGTGCCAAAAGAACTTGTTGATCAAAATCATCCTTTGCGATATAAACCTTTTGATCATATTGGATTGCTTCATTTCTTCCGTTCTAAAGAGGGCTTCAACTCCAAATCTCCTCTTAAAGACTATTGTGCAATCTAA

mRNA sequence

ATGGGTTCAGAAACGACGGAGGAGATCAGGCTGCCGGTGATAAATTTCAGTGATGAAAAAGTGAAGGCGGAGGGCGGCGTCGAGTGGCGGAGAACGAGCAAAAAAGTCCGGGAAGCCATGGAAGAGTACGGATGTTTTATAGCGAAGTATGAGGGAGTAAGTAAAGAACTTAGGGAGGAAGTGTTGAAGGCAGTGAAAGAACTTTTTGAGCTTCCAATTGAGAGGAAGAAGATGAATAAATATGAAAAGCCTTTTAATGGATACGTTGGAGAGCTTCGTACTCTTCCTCTTCATGAAAGCTTGGGAATTGATGATGCTACTAACTTCAATCAAGTTCAATCCTTCTCTCATCTCATGTGGCCGCCGGAGGGAAATCACCATTTTAGTGCAACTTTGCATTCATTTGCAAAGATAGCAGAAGAATTGGACAAAATGGTGACCAAAATGATATTTGAAAGCTATGGTGTGGAAAAGTATTACAATTCTCATGCTGATTCCATTACTTATCTTCTTAGAGTTTTAAAAAGCAAAGCTCCTCAATCTCCAGACCCTACTTTATGTCTTGTGGATCACACTGATAAGAGCTTCACCACCATGGTCTATCAAGATCATATCAATGGTTTGGAGCTCAAAACCAAAAATGGCCAATGGATTCAAGTTGACAACTCTTCACCCTCAAGCTTCCTCGTCATTGCTGGAGACGCAATCAAGAAGGCATGGAGCAATGATAGAATAGAATCACCAACTCATAAAGTGACGATAAGAGGAAATGAAGATAGGTATTCTCTTGCGATGTTTGGATTCAGCAATGGAATTGTGGAAGTGCCAAAAGAACTTGTTGATCAAAATCATCCTTTGCGATATAAACCTTTTGATCATATTGGATTGCTTCATTTCTTCCGTTCTAAAGAGGGCTTCAACTCCAAATCTCCTCTTAAAGACTATTGTGCAATCTAA

Coding sequence (CDS)

ATGGGTTCAGAAACGACGGAGGAGATCAGGCTGCCGGTGATAAATTTCAGTGATGAAAAAGTGAAGGCGGAGGGCGGCGTCGAGTGGCGGAGAACGAGCAAAAAAGTCCGGGAAGCCATGGAAGAGTACGGATGTTTTATAGCGAAGTATGAGGGAGTAAGTAAAGAACTTAGGGAGGAAGTGTTGAAGGCAGTGAAAGAACTTTTTGAGCTTCCAATTGAGAGGAAGAAGATGAATAAATATGAAAAGCCTTTTAATGGATACGTTGGAGAGCTTCGTACTCTTCCTCTTCATGAAAGCTTGGGAATTGATGATGCTACTAACTTCAATCAAGTTCAATCCTTCTCTCATCTCATGTGGCCGCCGGAGGGAAATCACCATTTTAGTGCAACTTTGCATTCATTTGCAAAGATAGCAGAAGAATTGGACAAAATGGTGACCAAAATGATATTTGAAAGCTATGGTGTGGAAAAGTATTACAATTCTCATGCTGATTCCATTACTTATCTTCTTAGAGTTTTAAAAAGCAAAGCTCCTCAATCTCCAGACCCTACTTTATGTCTTGTGGATCACACTGATAAGAGCTTCACCACCATGGTCTATCAAGATCATATCAATGGTTTGGAGCTCAAAACCAAAAATGGCCAATGGATTCAAGTTGACAACTCTTCACCCTCAAGCTTCCTCGTCATTGCTGGAGACGCAATCAAGAAGGCATGGAGCAATGATAGAATAGAATCACCAACTCATAAAGTGACGATAAGAGGAAATGAAGATAGGTATTCTCTTGCGATGTTTGGATTCAGCAATGGAATTGTGGAAGTGCCAAAAGAACTTGTTGATCAAAATCATCCTTTGCGATATAAACCTTTTGATCATATTGGATTGCTTCATTTCTTCCGTTCTAAAGAGGGCTTCAACTCCAAATCTCCTCTTAAAGACTATTGTGCAATCTAA

Protein sequence

MGSETTEEIRLPVINFSDEKVKAEGGVEWRRTSKKVREAMEEYGCFIAKYEGVSKELREEVLKAVKELFELPIERKKMNKYEKPFNGYVGELRTLPLHESLGIDDATNFNQVQSFSHLMWPPEGNHHFSATLHSFAKIAEELDKMVTKMIFESYGVEKYYNSHADSITYLLRVLKSKAPQSPDPTLCLVDHTDKSFTTMVYQDHINGLELKTKNGQWIQVDNSSPSSFLVIAGDAIKKAWSNDRIESPTHKVTIRGNEDRYSLAMFGFSNGIVEVPKELVDQNHPLRYKPFDHIGLLHFFRSKEGFNSKSPLKDYCAI*

Homology

[Close]

BLAST of CSPI07G21000 vs. ExPASy Swiss-Prot
Match: Q945B6 (Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=3702 GN=AOP1.2 PE=2 SV=1)

HSP 1 Score: 240.0 bits (611), Expect = 3.8e-62
Identity = 129/315 (40.95%), Postives = 194/315 (61.59%), Query Frame = 0


Query: 10  RLPVINFSDEKVKAEGGVEWRRTSKKVREAMEEYGCFIAKYEGVSKELREEVLKAVKELF 69
           +LPVI+FSD+ +K  G  +W      V +A+E+YGCF A ++ +S EL   V +A+++LF
Sbjct: 13  QLPVIDFSDQNLK-PGSSKWDEVKADVLKALEDYGCFEAFFDKLSVELNRSVFEAMEDLF 72

Query: 70  ELPIERKKMNKYEKPFNGYVGELRTLPLHESLGIDDATNFNQVQSFSHLMWPPEGNHHFS 129
           ELPI  K+ N   KPF+GY+       L+ESLGIDDA    +V  F+  +WP  GN   S
Sbjct: 73  ELPIPTKQRNVSSKPFHGYLCH----NLYESLGIDDANVLEKVNDFTQQLWPDHGNKSIS 132

Query: 130 ATLHSFAKIAEELDKMVTKMIFESYGVEKYYNSHADSITYLLRVLKSKAPQSPD-----P 189
            T+H F++   ELD MV +MI ES+G+E Y + H +S  YL R++K  +P   D      
Sbjct: 133 ETIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYTSPPDDDDDDEET 192

Query: 190 TLCLVDHTDKSFTTMVYQDHINGLELKTKNGQWIQVDNSSPSSFLVIAGDAIKKAWSNDR 249
            L L  HTDK+  T+++Q  ++GLE+KTK+ +WI+V   S  S LV+ GD++  A  N R
Sbjct: 193 KLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKV-KPSQDSVLVMVGDSL-CALLNGR 252

Query: 250 IESPTHKVTIRGNEDRYSLAMFGF--SNGIVEVPKELVDQNHPLRYKPFDHIGLLHFFRS 309
           + SP H+V + G + RYS  +F    +  I++ P+ELVD+ HP  +KPF++   LHFF++
Sbjct: 253 LHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFFQT 312

Query: 310 KEGFNSKSPLKDYCA 318
           + G  ++S L  + A
Sbjct: 313 EAGRIAQSALHAFAA 320

[Close]

BLAST of CSPI07G21000 vs. ExPASy Swiss-Prot
Match: Q9ZTA3 (Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 239.2 bits (609), Expect = 6.4e-62
Identity = 128/316 (40.51%), Postives = 195/316 (61.71%), Query Frame = 0


Query: 10  RLPVINFSDEKVKAEGGVEWRRTSKKVREAMEEYGCFIAKYEGVSKELREEVLKAVKELF 69
           +LPVI+FSD+ +K  G  +W   +  V +A+E+YGCF A ++ +S EL   V +A+++LF
Sbjct: 13  QLPVIDFSDQNLK-PGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDLF 72

Query: 70  ELPIERKKMNKYEKPFNGYVGELRTLPLHESLGIDDATNFNQVQSFSHLMWPPEGNHHFS 129
           ELPI  K+ N   KPF+GY+       L+ESLGI+DA    +V  F+  +WP  GN   S
Sbjct: 73  ELPIPTKQRNVSSKPFHGYLCH----NLYESLGINDANVLEKVNDFTQQLWPDHGNKSIS 132

Query: 130 ATLHSFAKIAEELDKMVTKMIFESYGVEKYYNSHADSITYLLRVLKSKAPQSPD------ 189
            T+H F++   ELD MV +MI ES+G+E Y + H +S  YL R++K  +P   D      
Sbjct: 133 ETIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYTSPPDDDDDDDEE 192

Query: 190 PTLCLVDHTDKSFTTMVYQDHINGLELKTKNGQWIQVDNSSPSSFLVIAGDAIKKAWSND 249
             L L  HTDK+  T+++Q  ++GLE+KTK+ +WI+V   S  S LV+ GD++  A  N 
Sbjct: 193 TKLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKV-KPSQDSVLVMVGDSL-CALLNG 252

Query: 250 RIESPTHKVTIRGNEDRYSLAMFGF--SNGIVEVPKELVDQNHPLRYKPFDHIGLLHFFR 309
           R+ SP H+V + G + RYS  +F    +  I++ P+ELVD+ HP  +KPF++   LHFF+
Sbjct: 253 RLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFFQ 312

Query: 310 SKEGFNSKSPLKDYCA 318
           ++ G  ++S L  + A
Sbjct: 313 TEAGRIAQSALHAFAA 321

[Close]

BLAST of CSPI07G21000 vs. ExPASy Swiss-Prot
Match: Q944Z5 (Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 233.8 bits (595), Expect = 2.7e-60
Identity = 125/302 (41.39%), Postives = 189/302 (62.58%), Query Frame = 0


Query: 10  RLPVINFSDEKVKAEGGVEWRRTSKKVREAMEEYGCFIAKYEGVSKELREEVLKAVKELF 69
           +LPVI+FSD+ +K  G  +W   +  V +A+E+YGCF A ++ +S EL   V +A+++LF
Sbjct: 6   QLPVIDFSDQNLK-PGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDLF 65

Query: 70  ELPIERKKMNKYEKPFNGYVGELRTLPLHESLGIDDATNFNQVQSFSHLMWPPEGNHHFS 129
           ELPI  K+ N   K F+GY+       L+ESLGI+DA    +V  F+  +WP  GN   S
Sbjct: 66  ELPIPTKQRNVSSKLFHGYLCH----NLYESLGINDANVLEKVNDFTQQLWPDHGNKSIS 125

Query: 130 ATLHSFAKIAEELDKMVTKMIFESYGVEKYYNSHADSITYLLRVLKSKAPQSPD----PT 189
            T+H F++   ELD MV +MI ES+G+EKY + H +S  YL R++K  +P   D      
Sbjct: 126 ETIHLFSEQLVELDLMVRRMIMESFGIEKYIDEHLNSTYYLTRLMKYTSPPDDDDDEETK 185

Query: 190 LCLVDHTDKSFTTMVYQDHINGLELKTKNGQWIQVDNSSPSSFLVIAGDAIKKAWSNDRI 249
           L L  HTDK+  T+++Q  ++GLE+KTK+ +WI+V   S  S LV+ GD++  A  N R+
Sbjct: 186 LGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKV-KPSQDSVLVMVGDSL-CALLNGRL 245

Query: 250 ESPTHKVTIRGNEDRYSLAMFGF--SNGIVEVPKELVDQNHPLRYKPFDHIGLLHFFRSK 306
            SP H+V + G + RYS  +F    +  I++ P+ELVD+ HP  +KPF++   LHFF+++
Sbjct: 246 HSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFFQTE 300

[Close]

BLAST of CSPI07G21000 vs. ExPASy Swiss-Prot
Match: A0A0N9HQ36 (Deoxypodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=2-ODD PE=1 SV=1)

HSP 1 Score: 228.4 bits (581), Expect = 1.1e-58
Identity = 125/318 (39.31%), Postives = 189/318 (59.43%), Query Frame = 0


Query: 1   MGSETTEEIRLPVINFSDEKVKAEGGVEWRRTSKKVREAMEEYGCFIAKYEGVSKELREE 60
           MGS  T  +RLPVI+ S + +K  G   W     +VREA+EEYGCF A  + VS EL++ 
Sbjct: 1   MGS--TAPLRLPVIDLSMKNLK-PGTTSWNSVRTQVREALEEYGCFEAVIDAVSPELQKA 60

Query: 61  VLKAVKELFELPIERKKMNKYEKPFNGYVGELRTLP-LHESLGIDDATNFNQVQSFSHLM 120
           V     EL  LP+E K +N  +  ++G+     ++P L+E +G+   T+  +V+ F++LM
Sbjct: 61  VCNKGHELLNLPLETKMLNGNKPEYDGFT----SIPNLNEGMGVGRITDLEKVERFTNLM 120

Query: 121 WPPEGNHHFSATLHSFAKIAEELDKMVTKMIFESYGVEKYYNSHADSITYLLRVLKSKAP 180
           W PEGN  F  T++S+ K   E+D ++  M+FES+G+EK+++S  +S  YLL  ++ + P
Sbjct: 121 W-PEGNKDFCETVYSYGKRMAEVDHILKMMVFESFGMEKHFDSFCESTNYLLHFMRYQQP 180

Query: 181 QSPDPTLCLVDHTDKSFTTMVYQDHINGLELKTKNGQWIQVDNSSPSSFLVIAGDAIKKA 240
                +  L  H DKS  T+V Q+ + GLE +TK+G+WI     +  + +V+ GD    A
Sbjct: 181 GKDGRSPALSLHKDKSILTIVNQNDVKGLEFETKDGEWIL---PTADNHIVLLGDCF-MA 240

Query: 241 WSNDRIESPTHKVTIRGNEDRYSLAMFGFSNGIVEVPKELVDQNHPLRYKPFDHIGLLHF 300
           WSN R+ SP H+VT+  N+ R S + F F   I+E P ELVD+ HPL + PF+   LL +
Sbjct: 241 WSNGRLHSPLHRVTLVANQARLSTSSFSFPKDIIETPAELVDEEHPLLFNPFEITELLAY 300

Query: 301 FRSKEGFNSKSPLKDYCA 318
             +KEG  +   LK Y A
Sbjct: 301 CFTKEGAKAVCDLKQYKA 306

[Close]

BLAST of CSPI07G21000 vs. ExPASy Swiss-Prot
Match: Q9ZTA1 (2-oxoglutarate-dependent dioxygenase AOP3 OS=Arabidopsis thaliana OX=3702 GN=AOP3 PE=2 SV=1)

HSP 1 Score: 198.0 bits (502), Expect = 1.6e-49
Identity = 133/408 (32.60%), Postives = 191/408 (46.81%), Query Frame = 0


Query: 10  RLPVINFSDEKVKAEGGVEWRRTSKKVREAMEEYGCFIAKYEGVSKELREEVLKAVKELF 69
           +LP+I  SD+ +K  G  +W +    VR+A+E+YGCF AK + VS EL+  VLKA++ELF
Sbjct: 7   QLPLICLSDQTLK-PGSSKWVKVRSDVRKALEDYGCFEAKIDQVSMELQGSVLKAMQELF 66

Query: 70  ELPIERKKMNKYEKPFNGYVGELRTLPLHESLGIDDATNFNQVQSFSHLMWPPEGNHHFS 129
            LP E K+ N   KPF GY   L    L ES GI DA    +   F+  +W PEGN   S
Sbjct: 67  ALPTEAKQRNVCPKPFTGY---LSHNGLSESFGIKDANILEKAHEFTQQLW-PEGNKSIS 126

Query: 130 ATLHSFAKIAEELDKMVTKMIFESYGVEKYYNSHADSITYLLRVLKSKAPQSPDPT---- 189
             +  +A+   ELD MV ++I ESYG+E + + H +S  Y +R++K  A    D T    
Sbjct: 127 KMIQLYAEKLAELDMMVRRLILESYGIEYFIDEHLNSTYYRMRLMKYIARPDNDITAAVG 186

Query: 190 ------------------------------------------------------------ 249
                                                                       
Sbjct: 187 ANVDNGANDNADGDANVNDDGASIGVKVNVDVGDDVNDNDSVNIGVGVDINVETNVNGDL 246

Query: 250 --------------------------------LCLVDHTDKSFTTMVYQDHINGLELKTK 309
                                           L L  HTDKS T ++YQ  I+GLE+KTK
Sbjct: 247 DAEANGDATAWVVGAVSGNASVGAKEANVDAELGLPSHTDKSLTGIIYQHQIDGLEVKTK 306

Query: 310 NGQWIQVDNSSPSSFLVIAGDAIKKAWSNDRIESPTHKVTI-RGNEDRYSLAMFG--FSN 319
            G+WI+V   +P++ +VIAGDA+  A  N RI SP H+V +    + RY+ A+F      
Sbjct: 307 EGKWIRV-KPAPNTVIVIAGDAL-CALMNGRIPSPYHRVRVTEKKKTRYAAALFSNPKEG 366

[Close]

BLAST of CSPI07G21000 vs. ExPASy TrEMBL
Match: A0A1S3CDV1 (probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103499759 PE=3 SV=1)

HSP 1 Score: 538.5 bits (1386), Expect = 1.9e-149
Identity = 264/318 (83.02%), Postives = 283/318 (88.99%), Query Frame = 0


Query: 1   MGSETTEEIRLPVINFSDEKVKAEGGVEWRRTSKKVREAMEEYGCFIAKYEGVSKELREE 60
           MGSET EE RLPVINFSDEKVKAEG VEWRRTSKKVREA+EEYGCFIAKYEGV KELREE
Sbjct: 1   MGSETMEE-RLPVINFSDEKVKAEGSVEWRRTSKKVREALEEYGCFIAKYEGVGKELREE 60

Query: 61  VLKAVKELFELPIERKKMNKYEKPFNGYVGELRTLPLHESLGIDDATNFNQVQSFSHLMW 120
           VLKAVKELFELPIERKKMNKYEKPFNGYVGELRTLPLHESLGIDDATNFN VQSFSHLMW
Sbjct: 61  VLKAVKELFELPIERKKMNKYEKPFNGYVGELRTLPLHESLGIDDATNFNGVQSFSHLMW 120

Query: 121 PPEGNHHFSATLHSFAKIAEELDKMVTKMIFESYGVEKYYNSHADSITYLLRVLKSKAPQ 180
            PEGNHHFSATLHSFAK AEELDKMVTKMIFESYGVEKYYNSHADSITYLLR+LK+KAPQ
Sbjct: 121 -PEGNHHFSATLHSFAKRAEELDKMVTKMIFESYGVEKYYNSHADSITYLLRILKNKAPQ 180

Query: 181 SPDPTLCLVDHTDKSFTTMVYQDHINGLELKTKNGQWIQVDNSSPSSFLVIAGDAIKKAW 240
           SPDPTLCLVDHTDKSFTTM+YQDHINGLELKTKNGQWIQVDNSSPSSF+VIAGDA  KAW
Sbjct: 181 SPDPTLCLVDHTDKSFTTMIYQDHINGLELKTKNGQWIQVDNSSPSSFVVIAGDAF-KAW 240

Query: 241 SNDRIESPTHKVTIRGNEDRYSLAMFGFSNGIVEVPKELVDQNHPLRYKPFDHIGLLHFF 300
           SN+RI S  H+V +RG E RYS+ MF F NG +EVP+  VD  +PLRYKPF+H   L + 
Sbjct: 241 SNERIRSCRHRVIMRGEETRYSIGMFSFKNGTIEVPQGFVDDANPLRYKPFNHYDFLTYD 300

Query: 301 RSKEGFNSKSPLKDYCAI 319
           ++    N+ S +KDYC +
Sbjct: 301 KANASHNTISRIKDYCGL 315

[Close]

BLAST of CSPI07G21000 vs. ExPASy TrEMBL
Match: A0A6J1GRH7 (probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 OS=Cucurbita moschata OX=3662 GN=LOC111456372 PE=4 SV=1)

HSP 1 Score: 533.5 bits (1373), Expect = 6.1e-148
Identity = 260/320 (81.25%), Postives = 286/320 (89.38%), Query Frame = 0


Query: 1   MGSET--TEEIRLPVINFSDEKVKAEGGVEWRRTSKKVREAMEEYGCFIAKYEGVSKELR 60
           MGSET    +  LPVINFS + VKAEGG  WRR S +VREA+EEYGCFIA+YEG+S ELR
Sbjct: 1   MGSETGPRMDAGLPVINFSGDDVKAEGGEGWRRRSGEVREALEEYGCFIAEYEGISPELR 60

Query: 61  EEVLKAVKELFELPIERKKMNKYEKPFNGYVGELRTLPLHESLGIDDATNFNQVQSFSHL 120
            EV KAV+ LFELPIE KK N+YEKPFNGYVGELRTLPLHESLGIDDATN + VQ+F+HL
Sbjct: 61  VEVFKAVEALFELPIETKKKNRYEKPFNGYVGELRTLPLHESLGIDDATNIDGVQAFTHL 120

Query: 121 MWPPEGNHHFSATLHSFAKIAEELDKMVTKMIFESYGVEKYYNSHADSITYLLRVLKSKA 180
           MW P GNHHFS+ LHSFAK AEELD+MVTKMIFESYGVEKY+ +HA SITYLLR LK+KA
Sbjct: 121 MW-PNGNHHFSSNLHSFAKKAEELDQMVTKMIFESYGVEKYHETHAASITYLLRALKNKA 180

Query: 181 PQSPDPTLCLVDHTDKSFTTMVYQDHINGLELKTKNGQWIQVDNSSPSSFLVIAGDAIKK 240
           P S +PTLCLVDHTDKSFTTMVYQDH NGLE++TK+G+WIQVDNSSPS+F+VIAGDA  K
Sbjct: 181 PHSQNPTLCLVDHTDKSFTTMVYQDHTNGLEVQTKDGRWIQVDNSSPSTFVVIAGDAF-K 240

Query: 241 AWSNDRIESPTHKVTIRGNEDRYSLAMFGFSNGIVEVPKELVDQNHPLRYKPFDHIGLLH 300
           AWSNDRIESPTHKVTIRGNEDRYSLAMFGFSNGIVEVPKEL+DQ HPLRYKPFDHIGLLH
Sbjct: 241 AWSNDRIESPTHKVTIRGNEDRYSLAMFGFSNGIVEVPKELIDQTHPLRYKPFDHIGLLH 300

Query: 301 FFRSKEGFNSKSPLKDYCAI 319
           FFRSKEGF SKSPL+DYCA+
Sbjct: 301 FFRSKEGFKSKSPLQDYCAL 318

[Close]

BLAST of CSPI07G21000 vs. ExPASy TrEMBL
Match: A0A6J1JM78 (probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 OS=Cucurbita maxima OX=3661 GN=LOC111488145 PE=4 SV=1)

HSP 1 Score: 529.6 bits (1363), Expect = 8.8e-147
Identity = 258/320 (80.62%), Postives = 286/320 (89.38%), Query Frame = 0


Query: 1   MGSET--TEEIRLPVINFSDEKVKAEGGVEWRRTSKKVREAMEEYGCFIAKYEGVSKELR 60
           MGSET    +  LPVINFS + VKAEGG  WRR S +VREA+EEYGCFIA+YEG+S ELR
Sbjct: 1   MGSETGPRMDAGLPVINFSGDDVKAEGGEVWRRRSGEVREALEEYGCFIAEYEGISPELR 60

Query: 61  EEVLKAVKELFELPIERKKMNKYEKPFNGYVGELRTLPLHESLGIDDATNFNQVQSFSHL 120
            +V KAV+ LFELPIE KK N+YEKPFNGYVGELRTLPLHESLGIDDATN + VQ+F++L
Sbjct: 61  VQVFKAVEALFELPIETKKKNRYEKPFNGYVGELRTLPLHESLGIDDATNIDGVQAFTNL 120

Query: 121 MWPPEGNHHFSATLHSFAKIAEELDKMVTKMIFESYGVEKYYNSHADSITYLLRVLKSKA 180
           MW P GNHHFS+ LHSFAK AEELD+MVTKMIFESYGVEKY+ +HA SITYLLR LK+KA
Sbjct: 121 MW-PNGNHHFSSNLHSFAKKAEELDQMVTKMIFESYGVEKYHETHAASITYLLRALKNKA 180

Query: 181 PQSPDPTLCLVDHTDKSFTTMVYQDHINGLELKTKNGQWIQVDNSSPSSFLVIAGDAIKK 240
           P S +PTLCLVDHTDKSFTTMVYQDH NGLE++TK+G+WIQVDNSSPS+F+VIAGDA  K
Sbjct: 181 PHSQNPTLCLVDHTDKSFTTMVYQDHTNGLEVQTKDGRWIQVDNSSPSTFVVIAGDAF-K 240

Query: 241 AWSNDRIESPTHKVTIRGNEDRYSLAMFGFSNGIVEVPKELVDQNHPLRYKPFDHIGLLH 300
           AWSNDRIESPTHKVTIRGNEDRYSLAMFGFSNGIVEVPKEL+DQ HPLRYKPFDHIGLLH
Sbjct: 241 AWSNDRIESPTHKVTIRGNEDRYSLAMFGFSNGIVEVPKELIDQTHPLRYKPFDHIGLLH 300

Query: 301 FFRSKEGFNSKSPLKDYCAI 319
           FFRSKEGF SKSPL+DYCA+
Sbjct: 301 FFRSKEGFKSKSPLQDYCAL 318

[Close]

BLAST of CSPI07G21000 vs. ExPASy TrEMBL
Match: A0A6J1DBW9 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Momordica charantia OX=3673 GN=LOC111019607 PE=3 SV=1)

HSP 1 Score: 508.4 bits (1308), Expect = 2.1e-140
Identity = 243/310 (78.39%), Postives = 278/310 (89.68%), Query Frame = 0


Query: 9   IRLPVINFSDEKVKAEGGVEWRRTSKKVREAMEEYGCFIAKYEGVSKELREEVLKAVKEL 68
           + LPVIN S + ++  GG  W+ T KKVREA+EEYGCF+A+Y GVS ELRE+V KA+++L
Sbjct: 29  VGLPVINLSGDDLR-PGGERWKTTGKKVREAVEEYGCFMAEYNGVSPELREQVFKALEQL 88

Query: 69  FELPIERKKMNKYEKPFNGYVGELRTLPLHESLGIDDATNFNQVQSFSHLMWPPEGNHHF 128
           F+LP+ERK  NKYEKPFNGYVG+LRTLPLHESLGIDDAT F+ V SF++LMW P GNHHF
Sbjct: 89  FDLPVERKMKNKYEKPFNGYVGQLRTLPLHESLGIDDATTFHGVHSFTNLMW-PSGNHHF 148

Query: 129 SATLHSFAKIAEELDKMVTKMIFESYGVEKYYNSHADSITYLLRVLKSKAPQSPDPTLCL 188
           SATLHSFAKIAE LDKMVTKMIFESYGVEKYY+SHA SITYLLRVLK+KAPQS +P+LCL
Sbjct: 149 SATLHSFAKIAEALDKMVTKMIFESYGVEKYYDSHASSITYLLRVLKNKAPQSENPSLCL 208

Query: 189 VDHTDKSFTTMVYQDHINGLELKTKNGQWIQVDNSSPSSFLVIAGDAIKKAWSNDRIESP 248
           VDHTDKSFTTM+YQDHINGLE+KTK+GQWI+V+ SSPS+F+VIAGDA  KAWSN RIESP
Sbjct: 209 VDHTDKSFTTMLYQDHINGLEVKTKDGQWIKVEISSPSNFIVIAGDAF-KAWSNGRIESP 268

Query: 249 THKVTIRGNEDRYSLAMFGFSNGIVEVPKELVDQNHPLRYKPFDHIGLLHFFRSKEGFNS 308
           THKV  RGNEDRYS+ MFGFSNG VEVPKELVDQNHPL+YKPF+HIGLL FF+S+EGFNS
Sbjct: 269 THKVMTRGNEDRYSVGMFGFSNGTVEVPKELVDQNHPLKYKPFNHIGLLRFFQSQEGFNS 328

Query: 309 KSPLKDYCAI 319
           KSPLKDYCA+
Sbjct: 329 KSPLKDYCAL 335

[Close]

BLAST of CSPI07G21000 vs. ExPASy TrEMBL
Match: A0A0A0K746 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G434950 PE=4 SV=1)

HSP 1 Score: 479.6 bits (1233), Expect = 1.0e-131
Identity = 236/237 (99.58%), Postives = 236/237 (99.58%), Query Frame = 0


Query: 1   MGSETTEEIRLPVINFSDEKVKAEGGVEWRRTSKKVREAMEEYGCFIAKYEGVSKELREE 60
           MGSETTEEIRLPVINFSDEKVKAEGGVEWRRTSK VREAMEEYGCFIAKYEGVSKELREE
Sbjct: 1   MGSETTEEIRLPVINFSDEKVKAEGGVEWRRTSKNVREAMEEYGCFIAKYEGVSKELREE 60

Query: 61  VLKAVKELFELPIERKKMNKYEKPFNGYVGELRTLPLHESLGIDDATNFNQVQSFSHLMW 120
           VLKAVKELFELPIERKKMNKYEKPFNGYVGELRTLPLHESLGIDDATNFNQVQSFSHLMW
Sbjct: 61  VLKAVKELFELPIERKKMNKYEKPFNGYVGELRTLPLHESLGIDDATNFNQVQSFSHLMW 120

Query: 121 PPEGNHHFSATLHSFAKIAEELDKMVTKMIFESYGVEKYYNSHADSITYLLRVLKSKAPQ 180
           PPEGNHHFSATLHSFAKIAEELDKMVTKMIFESYGVEKYYNSHADSITYLLRVLKSKAPQ
Sbjct: 121 PPEGNHHFSATLHSFAKIAEELDKMVTKMIFESYGVEKYYNSHADSITYLLRVLKSKAPQ 180

Query: 181 SPDPTLCLVDHTDKSFTTMVYQDHINGLELKTKNGQWIQVDNSSPSSFLVIAGDAIK 238
           SPDPTLCLVDHTDKSFTTMVYQDHINGLELKTKNGQWIQVDNSSPSSFLVIAGDAIK
Sbjct: 181 SPDPTLCLVDHTDKSFTTMVYQDHINGLELKTKNGQWIQVDNSSPSSFLVIAGDAIK 237

[Close]

BLAST of CSPI07G21000 vs. NCBI nr
Match: XP_004136074.1 (2-oxoglutarate-dependent dioxygenase AOP2 isoform X1 [Cucumis sativus] >KAE8646455.1 hypothetical protein Csa_015846 [Cucumis sativus])

HSP 1 Score: 647.5 bits (1669), Expect = 6.0e-182
Identity = 316/318 (99.37%), Postives = 316/318 (99.37%), Query Frame = 0


Query: 1   MGSETTEEIRLPVINFSDEKVKAEGGVEWRRTSKKVREAMEEYGCFIAKYEGVSKELREE 60
           MGSETTEEIRLPVINFSDEKVKAEGGVEWRRTSK VREAMEEYGCFIAKYEGVSKELREE
Sbjct: 1   MGSETTEEIRLPVINFSDEKVKAEGGVEWRRTSKNVREAMEEYGCFIAKYEGVSKELREE 60

Query: 61  VLKAVKELFELPIERKKMNKYEKPFNGYVGELRTLPLHESLGIDDATNFNQVQSFSHLMW 120
           VLKAVKELFELPIERKKMNKYEKPFNGYVGELRTLPLHESLGIDDATNFNQVQSFSHLMW
Sbjct: 61  VLKAVKELFELPIERKKMNKYEKPFNGYVGELRTLPLHESLGIDDATNFNQVQSFSHLMW 120

Query: 121 PPEGNHHFSATLHSFAKIAEELDKMVTKMIFESYGVEKYYNSHADSITYLLRVLKSKAPQ 180
           PPEGNHHFSATLHSFAKIAEELDKMVTKMIFESYGVEKYYNSHADSITYLLRVLKSKAPQ
Sbjct: 121 PPEGNHHFSATLHSFAKIAEELDKMVTKMIFESYGVEKYYNSHADSITYLLRVLKSKAPQ 180

Query: 181 SPDPTLCLVDHTDKSFTTMVYQDHINGLELKTKNGQWIQVDNSSPSSFLVIAGDAIKKAW 240
           SPDPTLCLVDHTDKSFTTMVYQDHINGLELKTKNGQWIQVDNSSPSSFLVIAGDAI KAW
Sbjct: 181 SPDPTLCLVDHTDKSFTTMVYQDHINGLELKTKNGQWIQVDNSSPSSFLVIAGDAI-KAW 240

Query: 241 SNDRIESPTHKVTIRGNEDRYSLAMFGFSNGIVEVPKELVDQNHPLRYKPFDHIGLLHFF 300
           SNDRIESPTHKVTIRGNEDRYSLAMFGFSNGIVEVPKELVDQNHPLRYKPFDHIGLLHFF
Sbjct: 241 SNDRIESPTHKVTIRGNEDRYSLAMFGFSNGIVEVPKELVDQNHPLRYKPFDHIGLLHFF 300

Query: 301 RSKEGFNSKSPLKDYCAI 319
           RSKEGFNSKSPLKDYCAI
Sbjct: 301 RSKEGFNSKSPLKDYCAI 317

[Close]

BLAST of CSPI07G21000 vs. NCBI nr
Match: XP_038896850.1 (probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 [Benincasa hispida])

HSP 1 Score: 561.2 bits (1445), Expect = 5.6e-156
Identity = 274/318 (86.16%), Postives = 293/318 (92.14%), Query Frame = 0


Query: 1   MGSETTEEIRLPVINFSDEKVKAEGGVEWRRTSKKVREAMEEYGCFIAKYEGVSKELREE 60
           MGSET     LPVINFS   VK EGG EWRR S++VREA+EEYGCFIA+Y+GVS+ELR E
Sbjct: 1   MGSETG---GLPVINFSGNGVKTEGGDEWRRRSREVREALEEYGCFIAEYDGVSRELRGE 60

Query: 61  VLKAVKELFELPIERKKMNKYEKPFNGYVGELRTLPLHESLGIDDATNFNQVQSFSHLMW 120
           V+KAVK LFELPIERKK NKYEKPFNGYVGELRTLPLHESLGIDDATNF+ VQSF+HLMW
Sbjct: 61  VMKAVKALFELPIERKKKNKYEKPFNGYVGELRTLPLHESLGIDDATNFHGVQSFTHLMW 120

Query: 121 PPEGNHHFSATLHSFAKIAEELDKMVTKMIFESYGVEKYYNSHADSITYLLRVLKSKAPQ 180
            P GNHHFS TLHSFAKIAEELDKMVTKMIFESYGVEKY+ SHADSITYLLR+LK+KAP 
Sbjct: 121 -PSGNHHFSTTLHSFAKIAEELDKMVTKMIFESYGVEKYHKSHADSITYLLRILKNKAPH 180

Query: 181 SPDPTLCLVDHTDKSFTTMVYQDHINGLELKTKNGQWIQVDNSSPSSFLVIAGDAIKKAW 240
           S +PTLCLVDHTDKSFTTM+YQDHINGLE+KTK+GQWIQVDNSSPS+F+VIAGDA  KAW
Sbjct: 181 SSNPTLCLVDHTDKSFTTMLYQDHINGLEVKTKHGQWIQVDNSSPSTFVVIAGDAF-KAW 240

Query: 241 SNDRIESPTHKVTIRGNEDRYSLAMFGFSNGIVEVPKELVDQNHPLRYKPFDHIGLLHFF 300
           SNDRIESPTHKV IRGNEDRYSLAMFGFSNGIVEVPKELVDQ HPLRYKPFDHIGLLHFF
Sbjct: 241 SNDRIESPTHKVMIRGNEDRYSLAMFGFSNGIVEVPKELVDQTHPLRYKPFDHIGLLHFF 300

Query: 301 RSKEGFNSKSPLKDYCAI 319
           RSKEGFNSKSPLKDYCA+
Sbjct: 301 RSKEGFNSKSPLKDYCAL 313

[Close]

BLAST of CSPI07G21000 vs. NCBI nr
Match: XP_031744406.1 (2-oxoglutarate-dependent dioxygenase AOP3 isoform X2 [Cucumis sativus])

HSP 1 Score: 543.1 bits (1398), Expect = 1.6e-150
Identity = 273/318 (85.85%), Postives = 273/318 (85.85%), Query Frame = 0


Query: 1   MGSETTEEIRLPVINFSDEKVKAEGGVEWRRTSKKVREAMEEYGCFIAKYEGVSKELREE 60
           MGSETTEEIRLPVINFSDEKVKAEGGVEWRRTSK VREAMEEYGCFIAKYEGVSKELREE
Sbjct: 1   MGSETTEEIRLPVINFSDEKVKAEGGVEWRRTSKNVREAMEEYGCFIAKYEGVSKELREE 60

Query: 61  VLKAVKELFELPIERKKMNKYEKPFNGYVGELRTLPLHESLGIDDATNFNQVQSFSHLMW 120
           VLKAVKELFELPIERKKMNKYEKPFNGYVGELRTLPLHESLGIDDATNFNQVQSFSHLMW
Sbjct: 61  VLKAVKELFELPIERKKMNKYEKPFNGYVGELRTLPLHESLGIDDATNFNQVQSFSHLMW 120

Query: 121 PPEGNHHFSATLHSFAKIAEELDKMVTKMIFESYGVEKYYNSHADSITYLLRVLKSKAPQ 180
           PPEGNHHF                                           RVLKSKAPQ
Sbjct: 121 PPEGNHHF-------------------------------------------RVLKSKAPQ 180

Query: 181 SPDPTLCLVDHTDKSFTTMVYQDHINGLELKTKNGQWIQVDNSSPSSFLVIAGDAIKKAW 240
           SPDPTLCLVDHTDKSFTTMVYQDHINGLELKTKNGQWIQVDNSSPSSFLVIAGDAI KAW
Sbjct: 181 SPDPTLCLVDHTDKSFTTMVYQDHINGLELKTKNGQWIQVDNSSPSSFLVIAGDAI-KAW 240

Query: 241 SNDRIESPTHKVTIRGNEDRYSLAMFGFSNGIVEVPKELVDQNHPLRYKPFDHIGLLHFF 300
           SNDRIESPTHKVTIRGNEDRYSLAMFGFSNGIVEVPKELVDQNHPLRYKPFDHIGLLHFF
Sbjct: 241 SNDRIESPTHKVTIRGNEDRYSLAMFGFSNGIVEVPKELVDQNHPLRYKPFDHIGLLHFF 274

Query: 301 RSKEGFNSKSPLKDYCAI 319
           RSKEGFNSKSPLKDYCAI
Sbjct: 301 RSKEGFNSKSPLKDYCAI 274

[Close]

BLAST of CSPI07G21000 vs. NCBI nr
Match: XP_008461058.1 (PREDICTED: probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 isoform X2 [Cucumis melo])

HSP 1 Score: 538.5 bits (1386), Expect = 3.9e-149
Identity = 264/318 (83.02%), Postives = 283/318 (88.99%), Query Frame = 0


Query: 1   MGSETTEEIRLPVINFSDEKVKAEGGVEWRRTSKKVREAMEEYGCFIAKYEGVSKELREE 60
           MGSET EE RLPVINFSDEKVKAEG VEWRRTSKKVREA+EEYGCFIAKYEGV KELREE
Sbjct: 1   MGSETMEE-RLPVINFSDEKVKAEGSVEWRRTSKKVREALEEYGCFIAKYEGVGKELREE 60

Query: 61  VLKAVKELFELPIERKKMNKYEKPFNGYVGELRTLPLHESLGIDDATNFNQVQSFSHLMW 120
           VLKAVKELFELPIERKKMNKYEKPFNGYVGELRTLPLHESLGIDDATNFN VQSFSHLMW
Sbjct: 61  VLKAVKELFELPIERKKMNKYEKPFNGYVGELRTLPLHESLGIDDATNFNGVQSFSHLMW 120

Query: 121 PPEGNHHFSATLHSFAKIAEELDKMVTKMIFESYGVEKYYNSHADSITYLLRVLKSKAPQ 180
            PEGNHHFSATLHSFAK AEELDKMVTKMIFESYGVEKYYNSHADSITYLLR+LK+KAPQ
Sbjct: 121 -PEGNHHFSATLHSFAKRAEELDKMVTKMIFESYGVEKYYNSHADSITYLLRILKNKAPQ 180

Query: 181 SPDPTLCLVDHTDKSFTTMVYQDHINGLELKTKNGQWIQVDNSSPSSFLVIAGDAIKKAW 240
           SPDPTLCLVDHTDKSFTTM+YQDHINGLELKTKNGQWIQVDNSSPSSF+VIAGDA  KAW
Sbjct: 181 SPDPTLCLVDHTDKSFTTMIYQDHINGLELKTKNGQWIQVDNSSPSSFVVIAGDAF-KAW 240

Query: 241 SNDRIESPTHKVTIRGNEDRYSLAMFGFSNGIVEVPKELVDQNHPLRYKPFDHIGLLHFF 300
           SN+RI S  H+V +RG E RYS+ MF F NG +EVP+  VD  +PLRYKPF+H   L + 
Sbjct: 241 SNERIRSCRHRVIMRGEETRYSIGMFSFKNGTIEVPQGFVDDANPLRYKPFNHYDFLTYD 300

Query: 301 RSKEGFNSKSPLKDYCAI 319
           ++    N+ S +KDYC +
Sbjct: 301 KANASHNTISRIKDYCGL 315

[Close]

BLAST of CSPI07G21000 vs. NCBI nr
Match: XP_022953974.1 (probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 [Cucurbita moschata])

HSP 1 Score: 533.5 bits (1373), Expect = 1.3e-147
Identity = 260/320 (81.25%), Postives = 286/320 (89.38%), Query Frame = 0


Query: 1   MGSET--TEEIRLPVINFSDEKVKAEGGVEWRRTSKKVREAMEEYGCFIAKYEGVSKELR 60
           MGSET    +  LPVINFS + VKAEGG  WRR S +VREA+EEYGCFIA+YEG+S ELR
Sbjct: 1   MGSETGPRMDAGLPVINFSGDDVKAEGGEGWRRRSGEVREALEEYGCFIAEYEGISPELR 60

Query: 61  EEVLKAVKELFELPIERKKMNKYEKPFNGYVGELRTLPLHESLGIDDATNFNQVQSFSHL 120
            EV KAV+ LFELPIE KK N+YEKPFNGYVGELRTLPLHESLGIDDATN + VQ+F+HL
Sbjct: 61  VEVFKAVEALFELPIETKKKNRYEKPFNGYVGELRTLPLHESLGIDDATNIDGVQAFTHL 120

Query: 121 MWPPEGNHHFSATLHSFAKIAEELDKMVTKMIFESYGVEKYYNSHADSITYLLRVLKSKA 180
           MW P GNHHFS+ LHSFAK AEELD+MVTKMIFESYGVEKY+ +HA SITYLLR LK+KA
Sbjct: 121 MW-PNGNHHFSSNLHSFAKKAEELDQMVTKMIFESYGVEKYHETHAASITYLLRALKNKA 180

Query: 181 PQSPDPTLCLVDHTDKSFTTMVYQDHINGLELKTKNGQWIQVDNSSPSSFLVIAGDAIKK 240
           P S +PTLCLVDHTDKSFTTMVYQDH NGLE++TK+G+WIQVDNSSPS+F+VIAGDA  K
Sbjct: 181 PHSQNPTLCLVDHTDKSFTTMVYQDHTNGLEVQTKDGRWIQVDNSSPSTFVVIAGDAF-K 240

Query: 241 AWSNDRIESPTHKVTIRGNEDRYSLAMFGFSNGIVEVPKELVDQNHPLRYKPFDHIGLLH 300
           AWSNDRIESPTHKVTIRGNEDRYSLAMFGFSNGIVEVPKEL+DQ HPLRYKPFDHIGLLH
Sbjct: 241 AWSNDRIESPTHKVTIRGNEDRYSLAMFGFSNGIVEVPKELIDQTHPLRYKPFDHIGLLH 300

Query: 301 FFRSKEGFNSKSPLKDYCAI 319
           FFRSKEGF SKSPL+DYCA+
Sbjct: 301 FFRSKEGFKSKSPLQDYCAL 318

[Close]

BLAST of CSPI07G21000 vs. TAIR 10
Match: AT1G52790.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 353.6 bits (906), Expect = 1.7e-97
Identity = 163/308 (52.92%), Postives = 230/308 (74.68%), Query Frame = 0


Query: 10  RLPVINFSDEKVKAEGGVEWRRTSKKVREAMEEYGCFIAKYEGVSK-ELREEVLKAVKEL 69
           ++P ++FS E +K  G   W  T + +R+A+EEYGCFI   +  +  +L + V  ++ +L
Sbjct: 4   KIPTLDFSREDLK-PGTKYWESTRENIRQALEEYGCFIIDLKDKTPLDLLDRVFGSLVDL 63

Query: 70  FELPIERKKMNKYEKPFNGYVGELRTLPLHESLGIDDATNFNQVQSFSHLMWPPEGNHHF 129
           F+LP + K  NKY+KP NGYVG++  LPLHESLGID+AT+    +SF+ LMW P+GN HF
Sbjct: 64  FDLPTQTKMKNKYDKPLNGYVGQIPALPLHESLGIDNATSLEATRSFTGLMW-PQGNEHF 123

Query: 130 SATLHSFAKIAEELDKMVTKMIFESYGVEKYYNSHADSITYLLRVLKSKAPQSPDPTLCL 189
           S  L+ +A+ A ELD+MVT+M+F+SY VEKYY+ + +S TYLLRVLK++AP + +PTL  
Sbjct: 124 SECLYKYAEFAAELDQMVTRMVFQSYNVEKYYDPYIESTTYLLRVLKNRAPNNENPTLGF 183

Query: 190 VDHTDKSFTTMVYQDHINGLELKTKNGQWIQVDNSSPSSFLVIAGDAIKKAWSNDRIESP 249
           V HTDKSFTT+++QD +NGLE++T+ G+ I ++ SSPS F+V+AGDA+  AWSNDR+ SP
Sbjct: 184 VTHTDKSFTTILHQDQVNGLEMETREGERININLSSPSLFMVVAGDAL-MAWSNDRVWSP 243

Query: 250 THKVTIRGNEDRYSLAMFGFSNGIVEVPKELVDQNHPLRYKPFDHIGLLHFFRSKEGFNS 309
            H+V + G  DRYSL MF F+NG ++VP+EL+D  HPL YKPFDH+GLL F+R+  G+ S
Sbjct: 244 RHQVLVSGETDRYSLGMFAFNNGTLQVPEELIDHQHPLMYKPFDHLGLLRFYRTDTGYKS 303

Query: 310 KSPLKDYC 317
           + P+K YC
Sbjct: 304 ECPIKAYC 308

[Close]

BLAST of CSPI07G21000 vs. TAIR 10
Match: AT1G52800.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 315.1 bits (806), Expect = 6.6e-86
Identity = 145/309 (46.93%), Postives = 212/309 (68.61%), Query Frame = 0


Query: 10  RLPVINFSDEKVKAEGGVEWRRTSKKVREAMEEYGCFIAKYEGVSKELREEVLKAVKELF 69
           ++P+++ + ++        WR  S++  EA+EEYGCF+A Y+GV+++L + +  A +ELF
Sbjct: 9   KVPILDLTSQQELKPNTSTWRSVSREACEALEEYGCFLAVYDGVTQQLDDSIFAAAEELF 68

Query: 70  ELPIERKKMNKYEKPFNGYVGELRTLPLHESLGIDDATNFNQVQSFSHLMWPPEGNHHFS 129
           +LP E KK N  EKP++GYVG++  +PLHE LG+D  TN    Q F+HLMW P+GN  F 
Sbjct: 69  DLPTETKKKNVNEKPYHGYVGQMPVIPLHEGLGVDYVTNKEIAQRFTHLMW-PQGNDRFC 128

Query: 130 ATLHSFAKIAEELDKMVTKMIFESYGVEKYYNSHADSITYLLRVLKSKAPQSPDPTLCLV 189
            T+H+F+    ELD++V +MIFE+YGVEK+Y SH  S TYLL+ LK  AP          
Sbjct: 129 NTVHTFSNAVAELDRLVVRMIFENYGVEKHYESHVGSKTYLLKFLKYLAPPESISMPAFP 188

Query: 190 DHTDKSFTTMVYQDHINGLELKTKNGQWIQVDNSSPSSFLVIAGDAIKKAWSNDRIESPT 249
            HTDK+F ++++Q+ +NGLE+K+K+G+WI +    P S++V+AGD I   WSNDRI S  
Sbjct: 189 QHTDKTFLSILHQNDVNGLEVKSKDGEWISL-QLPPKSYVVMAGD-ISMGWSNDRIRSCE 248

Query: 250 HKVTIRGNEDRYSLAMFGFSNGIVEVPKELVDQNHPLRYKPFDHIGLLHFFRSKEGFNSK 309
           H+VT+ G++ RY+L +F F   +V +P+ELVD  HPL YKPFD+I L++F+ +KEG  + 
Sbjct: 249 HRVTMEGDKTRYTLGLFSFLTDLVSIPEELVDDKHPLMYKPFDNIALINFYTTKEGREAN 308

Query: 310 SPLKDYCAI 319
           S LK YC I
Sbjct: 309 STLKAYCGI 314

[Close]

BLAST of CSPI07G21000 vs. TAIR 10
Match: AT1G52820.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 274.2 bits (700), Expect = 1.3e-73
Identity = 142/321 (44.24%), Postives = 210/321 (65.42%), Query Frame = 0


Query: 1   MGSET-TEEIRLPVINFSDEKVKAEGGVEWRRTSKKVREAMEEYGCFIAKYEGVSKELRE 60
           MGSET    +RLPVI+FS++ +K  G  EW  T   V++A+++YG F A ++ +  ELR+
Sbjct: 1   MGSETPLLPLRLPVIDFSNKNLK-PGEPEWDLTRADVQKALQDYGYFEASFDRIPFELRK 60

Query: 61  EVLKAVKELFELPIERKKMNKYEKPFNGYVGELRTLPLHESLGIDDATNFNQVQSFSHLM 120
            V  A++ELF+LP++ K  N  +KPF+GYVG+   +PL+ES+GIDD+    +V +F+  +
Sbjct: 61  SVFGALEELFDLPLQTKLRNVSKKPFHGYVGQYPMVPLYESMGIDDSDIAEKVDAFTEKL 120

Query: 121 WPPEGNHHFSATLHSFAKIAEELDKMVTKMIFESYGVEKYYNSHADSITYLLRVLKSKAP 180
           W P+GN  FS T+ SF+K   ELD  + +MI ES+G++KY + H  S  YLLRV+K K P
Sbjct: 121 W-PQGNISFSTTIQSFSKKLSELDITIRRMIMESFGLDKYIDEHLHSTNYLLRVMKYKGP 180

Query: 181 QSPDPTLCLVDHTDKSFTTMVYQDHINGLELKTKNGQWIQVDNSSPSSFLVIAGDAIKKA 240
            + +  + L  HTDK+  T++YQ+H+ GLE++TK+  WI+V   +  SF V+ GD++  A
Sbjct: 181 DTEETKVGLNAHTDKNIVTILYQNHVEGLEVQTKDKNWIKV-KPTQDSFTVMIGDSL-YA 240

Query: 241 WSNDRIESPTHKVTIRGNEDRYSLAMFGF--SNGIVEVPKELVDQNHPLRYKPFDHIGLL 300
             N R+ SP H+V + G E RYSL +F    +  IV  P ELVD+ HP  +KPFDH+  L
Sbjct: 241 LLNGRLHSPYHRVMMTGTETRYSLGLFSIPKAGHIVSSPDELVDEEHPRLFKPFDHVEFL 300

Query: 301 HFFRSKEGFNSKSPLKDYCAI 319
            F+ ++ G  S+S LK YC I
Sbjct: 301 QFYYTEAGQRSQSALKTYCGI 317

[Close]

BLAST of CSPI07G21000 vs. TAIR 10
Match: AT1G15540.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 255.4 bits (651), Expect = 6.2e-68
Identity = 138/324 (42.59%), Postives = 195/324 (60.19%), Query Frame = 0


Query: 1   MGSETTEEIRLPVINFSDEKVKAEGGVEWRRTSKKVREAMEEYGCFIAKYEGVSKELREE 60
           MG   T E  LP+IN SD+ +K    + W  T   VREAME +G F+A+Y      L + 
Sbjct: 1   MGGNETRE--LPIINLSDKNLKQNTEL-WSFTRDSVREAMEHHGWFVAEYNNFPTGLHQS 60

Query: 61  VLKAVKELFELPIERKKMNKYEKPFNGYVGELRT-LPLHESLGIDDATNFNQVQSFSHLM 120
           +L+A KEL +LP+E K  N+  K  +GY+  +    P+HE LGID   +  Q + FS LM
Sbjct: 61  ILEAAKELLDLPVEIKLKNENHKAGHGYITMMSDGQPVHEGLGIDQVNDVQQCRGFSRLM 120

Query: 121 WPP--EGNHHFSATLHSFAKIAEELDKMVTKMIFESYGVEKYYNSHADSITYLLRVLKSK 180
           WP   + N  F  T+H++AK+  EL+++V +M+FESY VEKY   +     YLLR+LK +
Sbjct: 121 WPDDHDDNDRFCETVHAYAKMQAELEQLVIRMLFESYNVEKYTEKYIGGTRYLLRLLKYR 180

Query: 181 APQSPDPTLCLVDHTDKSFTTMVYQDHINGLELKT-KNGQWIQVDNSSPSSFLVIAGDAI 240
              + +P    + HTDKSF ++++Q+HI GL LK+ K   W      SP+ F+VIAGDAI
Sbjct: 181 RLPNGEPNRKFISHTDKSFISILHQNHITGLMLKSEKEDVWYHF-TPSPTRFVVIAGDAI 240

Query: 241 KKAWSNDRIESPTHKVTIRGNEDRYSLAMFGFSNGIVEVPKELVDQNHPLRYKPFDHIGL 300
             AWSNDRI++  HKV +   E RYSL  F F  G++  P+E+VD++HPL Y PF H GL
Sbjct: 241 -MAWSNDRIKACYHKVEMESVEMRYSLGFFSFQEGMISTPEEMVDKDHPLAYNPFHHDGL 300

Query: 301 LHFFRSKEGF--NSKSPLKDYCAI 319
           L ++ + E      ++  K YC I
Sbjct: 301 LEYYETLEVHLKAHRTMTKAYCGI 319

[Close]

BLAST of CSPI07G21000 vs. TAIR 10
Match: AT1G80320.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 255.0 bits (650), Expect = 8.0e-68
Identity = 136/321 (42.37%), Postives = 198/321 (61.68%), Query Frame = 0


Query: 4   ETTEEIRLPVINFSDEKVKAEGGVEWRRTSKKVREAMEEYGCFIAKYEGVSKELREEVLK 63
           ++ EE  LP+++FS +K+   G   W  T   VR AME  G F+A++ GVS +LR+ +L 
Sbjct: 2   DSHEEQCLPILDFSSDKL-VRGTSHWITTRDDVRRAMEGQGWFVAEFSGVSSDLRDNLLA 61

Query: 64  AVKELFELPIERKKMNKYEKPFNGYVG-ELRTLPLHESLGIDDATNFNQVQSFSHLMWPP 123
            +KE++ LP + K  N+  K  +GY+   +    +HESLGID AT     + FS L+W P
Sbjct: 62  GMKEMYYLPDQIKIKNENHKASHGYMSMVVDDYRIHESLGIDYATELQACKDFSKLLW-P 121

Query: 124 EGNHHFSATLHSFAKIAEELDKMVTKMIFESYGVE--KYYNSHADSITYLLRVLKSKAPQ 183
           +GN  F  T H +A    ELD+ V +M++ESYG++  K+  SH++S  YLLR+L  +  Q
Sbjct: 122 QGNDPFCQTTHMYAMTMAELDQTVMRMLYESYGMDEKKHSVSHSESTRYLLRMLSYRRQQ 181

Query: 184 SPDPTLCLVDHTDKSFTTMVYQDHINGLELKTKNGQWIQVDNSSPSSFLVIAGDAIKKAW 243
           + +     V HTDKSF ++++Q+H+ GL+LKT  GQW+   N SP+ F+V++G  +  AW
Sbjct: 182 NGEANTGFVSHTDKSFMSILHQNHVGGLQLKTMTGQWVGF-NPSPTRFVVLSGMGL-TAW 241

Query: 244 SNDRIESPTHKVTIRGNEDRYSLAMFGFSNGIVEVPKELVDQNHPLRYKPFDHIGLLHFF 303
           SNDRI++  HKV +  +E RYSL  F F  G +  P+ELVD  HPLRY PF+H GLL F+
Sbjct: 242 SNDRIKACYHKVVMSADEIRYSLGFFSFHKGTIRTPEELVDDQHPLRYNPFEHDGLLRFY 301

Query: 304 RSKEGFNSKSP---LKDYCAI 319
            S      KS    L+ YC +
Sbjct: 302 ESYINSLKKSSEDLLQIYCGV 318

The following BLAST results are available for this feature:

BLAST of CSPI07G21000 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q945B6	3.8e-62	40.95	Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=...	[more]
Q9ZTA3	6.4e-62	40.51	Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=37...	[more]
Q944Z5	2.7e-60	41.39	Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis tha...	[more]
A0A0N9HQ36	1.1e-58	39.31	Deoxypodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=2-ODD PE=...	[more]
Q9ZTA1	1.6e-49	32.60	2-oxoglutarate-dependent dioxygenase AOP3 OS=Arabidopsis thaliana OX=3702 GN=AOP...	[more]

BLAST of CSPI07G21000 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3CDV1	1.9e-149	83.02	probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 isoform X2 OS=Cucumi...	[more]
A0A6J1GRH7	6.1e-148	81.25	probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 OS=Cucurbita moschat...	[more]
A0A6J1JM78	8.8e-147	80.63	probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 OS=Cucurbita maxima ...	[more]
A0A6J1DBW9	2.1e-140	78.39	probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Momordica charantia OX=367...	[more]
A0A0A0K746	1.0e-131	99.58	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G434950 PE=4 SV=1	[more]

BLAST of CSPI07G21000 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004136074.1	6.0e-182	99.37	2-oxoglutarate-dependent dioxygenase AOP2 isoform X1 [Cucumis sativus] >KAE86464...	[more]
XP_038896850.1	5.6e-156	86.16	probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 [Benincasa hispida]	[more]
XP_031744406.1	1.6e-150	85.85	2-oxoglutarate-dependent dioxygenase AOP3 isoform X2 [Cucumis sativus]	[more]
XP_008461058.1	3.9e-149	83.02	PREDICTED: probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 isoform X...	[more]
XP_022953974.1	1.3e-147	81.25	probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 [Cucurbita moschata]	[more]

BLAST of CSPI07G21000 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G52790.1	1.7e-97	52.92	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G52800.1	6.6e-86	46.93	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G52820.1	1.3e-73	44.24	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G15540.1	6.2e-68	42.59	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G80320.1	8.0e-68	42.37	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 2..295 e-value: 3.9E-54 score: 186.4
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 11..90 e-value: 4.4E-13 score: 50.0
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 178..267 e-value: 1.8E-12 score: 47.7
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 8..317
None	No IPR available	PANTHER	PTHR47990:SF153	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN	coord: 8..317
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 6..300
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 145..269 score: 8.261216

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI07G21000.1	CSPI07G21000.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0046872	metal ion binding