CSPI07G19800 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI07G19800
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description4-coumarate--CoA ligase
LocationChr7: 17325318 .. 17327238 (-)
RNA-Seq ExpressionCSPI07G19800
SyntenyCSPI07G19800
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCCATCTCTACTTTTTTTCTCTTAATTACTCTCTGTTTCTACTCTGTCTTTCTTCAGACCTCAACAATGGCCTTTGAATCAAACCAAACTAATGAATTCATTTTCCGTTCAAAGCTTCCTAATATTCACATTCCCAATCATCTCCCACTTCACGATTATGTTTTTCAAAATCTCTCAAAATTTGCCTCACGCCCATGTTTGATCAACGGCGCCACCGGGGATGTTTACACCTACCATGACGTTCAGCTAACAGCCCGTCGAGTCGCCGCTGGCCTACACAACCTCGGTATTAAGAAGGGTGATGTTGTCATGAATTTACTTCCCAATTCCCCGGAGTTCGTCTTCACTTTCCTTGGCGCTTCTTACCGGGGCGCCATCATGACGGCAGCCAACCCTTTTTACACTGCGGTGGAAATAGCCAAACAGGCCAAAGCCGCCAATGCGAAATTAATCGTGACTATGGCTTGCTTCTACGATCGGGTTAAGGATTTGGGTGAAAATGGTGTCAAAATCGTATGTGTTGATTTTTCTGCTGAGGGTTGTTTGCATTTCTCTGTTTTGAGTGGGGCTGACGAATCTCTAATGTCGCGGGTGGATTTTTCTTCCGATGATGTGGTGGCGCTACCGTACTCCTCTGGGACTACCGGTTTGCCAAAGGGAGTTATGTTGACTCATAAAGGGTTAATCACAAGTGTGGCTCAACAAGTCGACGGCCAAAATCCGAACCTATATTATCGTGGCGATGATGTAATCCTCTGTGTACTGCCGCTTTTCCATATCTATTCACTCAATTCGATTTTGCTGTGTGGGCTACGCGCCGGTGCTGCCATTATGATTATGCAAAAGTTCGACATCGTTTCACTTTTTCAATTGATTGGGAAATACAAAATCTCGATCGTGCCCATCGTGCCGCCAATCTTCTTGGCCATTGCTAAGTCAGCTGAGTTTGAAAAATACGACGTGTCGTCCGTAAGGGTTCTAAAATCCGGCGGAGCACCACTGGGGAAGGAGTTGGTAGAGGCCGTGAAGGCAAAATTTCCAGCGGCGATTCTCGCACAAGGGTACGGAATGACTGAGGCTGGTCCGGTTCTAACTATGAGTTTGGCTTTTGCAAAAGAACCGTTTCAAGTGAAATCCGAAGCATGCGGAACTGTGGTCAGAAATGCAGAGATGAAGATAGTCGACCCGGAAACCGGCTCCTCCTTACCTGAAAATTCCGCAGGTGAGATTTGTATTAGAGGTGATCAAATCATGAAGGGATATTTGAATGATTTGGAGTCGACCAAGAGGACTATTGATAAAGAAGGATGGCTTCACACCGGCGACATCGGCTTCATCGATGACAACAATGAGCTCTTCATTATAGACCGGCTTAAGGAGCTTATCAAGTTCAAAACATTTCAAGTGGCACCCGCCGAACTGGAAGCACTTCTTATCACTCACCCAAAACTACGTGACGCTGCTGTTATTGGGTGAGTTCAAATCCTATTTGGTTCAAAATGGCATTTGTGAGATTAAATTTTTAATGGGATTTTTCTTTGCATATTGTTGTTTTTAGTATGCCAGACGTGGAGGCAGGAGAAGTACCGGTGGCGTTTGTGGTGGAGGAGAAGAGTGGTGCAAGTGCAACAACAACAGAAGAAGAAGTAAAAGAATTCATAGCAAAGCAAGTGATTTTCTACAAAAAGCTAAAGCGTGTGTTTTTTGTCAACGCTATTCCAAAGGCACCATCTGGCAAAATCCTCAGGAAAGAAATAAGGGCAAATTTGGCATCCGGCGCTTACAATTAATTAAACCCTAATCTTAGTTATTCATTTATTTATTAGCCTTCTGTTTGGTTTGTTAATTAATGCAAATTATGTATTAGGATTGTGAAACACAATTCTTACCTGTAATTGTATATTTTATTTTACAATAATAA

mRNA sequence

CCCATCTCTACTTTTTTTCTCTTAATTACTCTCTGTTTCTACTCTGTCTTTCTTCAGACCTCAACAATGGCCTTTGAATCAAACCAAACTAATGAATTCATTTTCCGTTCAAAGCTTCCTAATATTCACATTCCCAATCATCTCCCACTTCACGATTATGTTTTTCAAAATCTCTCAAAATTTGCCTCACGCCCATGTTTGATCAACGGCGCCACCGGGGATGTTTACACCTACCATGACGTTCAGCTAACAGCCCGTCGAGTCGCCGCTGGCCTACACAACCTCGGTATTAAGAAGGGTGATGTTGTCATGAATTTACTTCCCAATTCCCCGGAGTTCGTCTTCACTTTCCTTGGCGCTTCTTACCGGGGCGCCATCATGACGGCAGCCAACCCTTTTTACACTGCGGTGGAAATAGCCAAACAGGCCAAAGCCGCCAATGCGAAATTAATCGTGACTATGGCTTGCTTCTACGATCGGGTTAAGGATTTGGGTGAAAATGGTGTCAAAATCGTATGTGTTGATTTTTCTGCTGAGGGTTGTTTGCATTTCTCTGTTTTGAGTGGGGCTGACGAATCTCTAATGTCGCGGGTGGATTTTTCTTCCGATGATGTGGTGGCGCTACCGTACTCCTCTGGGACTACCGGTTTGCCAAAGGGAGTTATGTTGACTCATAAAGGGTTAATCACAAGTGTGGCTCAACAAGTCGACGGCCAAAATCCGAACCTATATTATCGTGGCGATGATGTAATCCTCTGTGTACTGCCGCTTTTCCATATCTATTCACTCAATTCGATTTTGCTGTGTGGGCTACGCGCCGGTGCTGCCATTATGATTATGCAAAAGTTCGACATCGTTTCACTTTTTCAATTGATTGGGAAATACAAAATCTCGATCGTGCCCATCGTGCCGCCAATCTTCTTGGCCATTGCTAAGTCAGCTGAGTTTGAAAAATACGACGTGTCGTCCGTAAGGGTTCTAAAATCCGGCGGAGCACCACTGGGGAAGGAGTTGGTAGAGGCCGTGAAGGCAAAATTTCCAGCGGCGATTCTCGCACAAGGGTACGGAATGACTGAGGCTGGTCCGGTTCTAACTATGAGTTTGGCTTTTGCAAAAGAACCGTTTCAAGTGAAATCCGAAGCATGCGGAACTGTGGTCAGAAATGCAGAGATGAAGATAGTCGACCCGGAAACCGGCTCCTCCTTACCTGAAAATTCCGCAGGTGAGATTTGTATTAGAGGTGATCAAATCATGAAGGGATATTTGAATGATTTGGAGTCGACCAAGAGGACTATTGATAAAGAAGGATGGCTTCACACCGGCGACATCGGCTTCATCGATGACAACAATGAGCTCTTCATTATAGACCGGCTTAAGGAGCTTATCAAGTTCAAAACATTTCAAGTGGCACCCGCCGAACTGGAAGCACTTCTTATCACTCACCCAAAACTACGTGACGCTGCTGTTATTGGTATGCCAGACGTGGAGGCAGGAGAAGTACCGGTGGCGTTTGTGGTGGAGGAGAAGAGTGGTGCAAGTGCAACAACAACAGAAGAAGAAGTAAAAGAATTCATAGCAAAGCAAGTGATTTTCTACAAAAAGCTAAAGCGTGTGTTTTTTGTCAACGCTATTCCAAAGGCACCATCTGGCAAAATCCTCAGGAAAGAAATAAGGGCAAATTTGGCATCCGGCGCTTACAATTAATTAAACCCTAATCTTAGTTATTCATTTATTTATTAGCCTTCTGTTTGGTTTGTTAATTAATGCAAATTATGTATTAGGATTGTGAAACACAATTCTTACCTGTAATTGTATATTTTATTTTACAATAATAA

Coding sequence (CDS)

ATGGCCTTTGAATCAAACCAAACTAATGAATTCATTTTCCGTTCAAAGCTTCCTAATATTCACATTCCCAATCATCTCCCACTTCACGATTATGTTTTTCAAAATCTCTCAAAATTTGCCTCACGCCCATGTTTGATCAACGGCGCCACCGGGGATGTTTACACCTACCATGACGTTCAGCTAACAGCCCGTCGAGTCGCCGCTGGCCTACACAACCTCGGTATTAAGAAGGGTGATGTTGTCATGAATTTACTTCCCAATTCCCCGGAGTTCGTCTTCACTTTCCTTGGCGCTTCTTACCGGGGCGCCATCATGACGGCAGCCAACCCTTTTTACACTGCGGTGGAAATAGCCAAACAGGCCAAAGCCGCCAATGCGAAATTAATCGTGACTATGGCTTGCTTCTACGATCGGGTTAAGGATTTGGGTGAAAATGGTGTCAAAATCGTATGTGTTGATTTTTCTGCTGAGGGTTGTTTGCATTTCTCTGTTTTGAGTGGGGCTGACGAATCTCTAATGTCGCGGGTGGATTTTTCTTCCGATGATGTGGTGGCGCTACCGTACTCCTCTGGGACTACCGGTTTGCCAAAGGGAGTTATGTTGACTCATAAAGGGTTAATCACAAGTGTGGCTCAACAAGTCGACGGCCAAAATCCGAACCTATATTATCGTGGCGATGATGTAATCCTCTGTGTACTGCCGCTTTTCCATATCTATTCACTCAATTCGATTTTGCTGTGTGGGCTACGCGCCGGTGCTGCCATTATGATTATGCAAAAGTTCGACATCGTTTCACTTTTTCAATTGATTGGGAAATACAAAATCTCGATCGTGCCCATCGTGCCGCCAATCTTCTTGGCCATTGCTAAGTCAGCTGAGTTTGAAAAATACGACGTGTCGTCCGTAAGGGTTCTAAAATCCGGCGGAGCACCACTGGGGAAGGAGTTGGTAGAGGCCGTGAAGGCAAAATTTCCAGCGGCGATTCTCGCACAAGGGTACGGAATGACTGAGGCTGGTCCGGTTCTAACTATGAGTTTGGCTTTTGCAAAAGAACCGTTTCAAGTGAAATCCGAAGCATGCGGAACTGTGGTCAGAAATGCAGAGATGAAGATAGTCGACCCGGAAACCGGCTCCTCCTTACCTGAAAATTCCGCAGGTGAGATTTGTATTAGAGGTGATCAAATCATGAAGGGATATTTGAATGATTTGGAGTCGACCAAGAGGACTATTGATAAAGAAGGATGGCTTCACACCGGCGACATCGGCTTCATCGATGACAACAATGAGCTCTTCATTATAGACCGGCTTAAGGAGCTTATCAAGTTCAAAACATTTCAAGTGGCACCCGCCGAACTGGAAGCACTTCTTATCACTCACCCAAAACTACGTGACGCTGCTGTTATTGGTATGCCAGACGTGGAGGCAGGAGAAGTACCGGTGGCGTTTGTGGTGGAGGAGAAGAGTGGTGCAAGTGCAACAACAACAGAAGAAGAAGTAAAAGAATTCATAGCAAAGCAAGTGATTTTCTACAAAAAGCTAAAGCGTGTGTTTTTTGTCAACGCTATTCCAAAGGCACCATCTGGCAAAATCCTCAGGAAAGAAATAAGGGCAAATTTGGCATCCGGCGCTTACAATTAA

Protein sequence

MAFESNQTNEFIFRSKLPNIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQLTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKAANAKLIVTMACFYDRVKDLGENGVKIVCVDFSAEGCLHFSVLSGADESLMSRVDFSSDDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGQNPNLYYRGDDVILCVLPLFHIYSLNSILLCGLRAGAAIMIMQKFDIVSLFQLIGKYKISIVPIVPPIFLAIAKSAEFEKYDVSSVRVLKSGGAPLGKELVEAVKAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVKSEACGTVVRNAEMKIVDPETGSSLPENSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGDIGFIDDNNELFIIDRLKELIKFKTFQVAPAELEALLITHPKLRDAAVIGMPDVEAGEVPVAFVVEEKSGASATTTEEEVKEFIAKQVIFYKKLKRVFFVNAIPKAPSGKILRKEIRANLASGAYN*
Homology
BLAST of CSPI07G19800 vs. ExPASy Swiss-Prot
Match: O24146 (4-coumarate--CoA ligase 2 OS=Nicotiana tabacum OX=4097 GN=4CL2 PE=1 SV=1)

HSP 1 Score: 769.6 bits (1986), Expect = 2.3e-221
Identity = 381/537 (70.95%), Postives = 451/537 (83.99%), Query Frame = 0

Query: 10  EFIFRSKLPNIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQLTARRVAAG 69
           + IFRSKLP+I+IPNHLPLH Y F+N+S+F+SRPCLINGA   +YTY DV+L +R+VAAG
Sbjct: 9   DIIFRSKLPDIYIPNHLPLHSYCFENISEFSSRPCLINGANKQIYTYADVELNSRKVAAG 68

Query: 70  LHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKAANAKLI 129
           LH  GI+  D +M LLPNSPEFVF F+GASY GAI T ANP +T  E+ KQAKA++AK+I
Sbjct: 69  LHKQGIQPKDTIMILLPNSPEFVFAFIGASYLGAISTMANPLFTPAEVVKQAKASSAKII 128

Query: 130 VTMACFYDRVKDLG-ENGVKIVCVDFSAEGCLHFSVLSGADESLMSRVDFSSDDVVALPY 189
           VT AC  ++VKD   EN VKI+C+D + EGCLHFSVL+ A+E  +  V+   DDVVALPY
Sbjct: 129 VTQACHVNKVKDYAFENDVKIICIDSAPEGCLHFSVLTQANEHDIPEVEIQPDDVVALPY 188

Query: 190 SSGTTGLPKGVMLTHKGLITSVAQQVDGQNPNLYYRGDDVILCVLPLFHIYSLNSILLCG 249
           SSGTTGLPKGVMLTHKGL+TSVAQQVDG+NPNLY   +DV+LCVLPLFHIYSLNS+LLCG
Sbjct: 189 SSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYIHSEDVMLCVLPLFHIYSLNSVLLCG 248

Query: 250 LRAGAAIMIMQKFDIVSLFQLIGKYKISIVPIVPPIFLAIAKSAEFEKYDVSSVRVLKSG 309
           LR GAAI+IMQKFDIVS  +LI +YK++I P VPPI LAIAKS   + YD+SSVR + SG
Sbjct: 249 LRVGAAILIMQKFDIVSFLELIQRYKVTIGPFVPPIVLAIAKSPMVDDYDLSSVRTVMSG 308

Query: 310 GAPLGKELVEAVKAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVKSEACGTVVRNAE 369
            APLGKEL + V+AKFP A L QGYGMTEAGPVL M LAFAKEPF++KS ACGTVVRNAE
Sbjct: 309 AAPLGKELEDTVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFEIKSGACGTVVRNAE 368

Query: 370 MKIVDPETGSSLPENSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGDIGFIDDNN 429
           MKIVDP+TG+SLP N +GEICIRGDQIMKGYLND E+T RTIDKEGWL+TGDIG+IDD++
Sbjct: 369 MKIVDPKTGNSLPRNQSGEICIRGDQIMKGYLNDPEATARTIDKEGWLYTGDIGYIDDDD 428

Query: 430 ELFIIDRLKELIKFKTFQVAPAELEALLITHPKLRDAAVIGMPDVEAGEVPVAFVVEEKS 489
           ELFI+DRLKELIK+K FQVAPAELEALL+ HP + DAAV+ M D +AGEVPVAFVV    
Sbjct: 429 ELFIVDRLKELIKYKGFQVAPAELEALLLNHPNISDAAVVPMKDEQAGEVPVAFVVRSN- 488

Query: 490 GASATTTEEEVKEFIAKQVIFYKKLKRVFFVNAIPKAPSGKILRKEIRANLASGAYN 546
              +T TE+EVK+FI+KQVIFYK++KRVFFV+AIPK+PSGKILRK++RA LA+G  N
Sbjct: 489 --GSTITEDEVKDFISKQVIFYKRIKRVFFVDAIPKSPSGKILRKDLRAKLAAGLPN 542

BLAST of CSPI07G19800 vs. ExPASy Swiss-Prot
Match: O24540 (4-coumarate--CoA ligase OS=Vanilla planifolia OX=51239 GN=4CL PE=3 SV=1)

HSP 1 Score: 764.6 bits (1973), Expect = 7.5e-220
Identity = 384/543 (70.72%), Postives = 450/543 (82.87%), Query Frame = 0

Query: 7   QTNEFIFRSKLPNIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQLTARRV 66
           Q  + IFRSKLP+I+IP +LPLH Y F+N+SKF+SRPCLINGAT +++TY DV+L +RRV
Sbjct: 10  QKKDIIFRSKLPDIYIPKNLPLHSYCFENISKFSSRPCLINGATDEIFTYADVELISRRV 69

Query: 67  AAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKAANA 126
            +GL  LGIK+GD +M LLPNSPEFVF FLGAS+ G+I T ANPF+T+ E+ KQAKA+NA
Sbjct: 70  GSGLSKLGIKQGDTIMILLPNSPEFVFAFLGASFIGSISTMANPFFTSTEVIKQAKASNA 129

Query: 127 KLIVTMACFYDRVKDLG-ENGVKIVCVDFS-----AEGCLHFSVLSGADESLMSRVDFSS 186
           KLI+T  C+ D+VKD   ENGVKI+ +D +     A   LHFS L+GADE+ M +V+ S 
Sbjct: 130 KLIITQGCYVDKVKDYACENGVKIISIDTTTTADDAANILHFSELTGADENEMPKVEISP 189

Query: 187 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGQNPNLYYRGDDVILCVLPLFHIYS 246
           D VVALPYSSGTTGLPKGVMLTHKGL+TSVAQQVDG+NPNLY   DDV+LCVLPLFHIYS
Sbjct: 190 DGVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYMHSDDVLLCVLPLFHIYS 249

Query: 247 LNSILLCGLRAGAAIMIMQKFDIVSLFQLIGKYKISIVPIVPPIFLAIAKSAEFEKYDVS 306
           LNS+LLCGLRAG+ I+IMQKF+IV   +LI KYK++I P VPPI LAIAKS   + YD+S
Sbjct: 250 LNSVLLCGLRAGSGILIMQKFEIVPFLELIQKYKVTIGPFVPPIVLAIAKSTVVDNYDLS 309

Query: 307 SVRVLKSGGAPLGKELVEAVKAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVKSEAC 366
           SVR + SG APLGKEL +AV+AKFP A L QGYGMTEAGPVL M LAFAKEPF +KS AC
Sbjct: 310 SVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGAC 369

Query: 367 GTVVRNAEMKIVDPETGSSLPENSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 426
           GTVVRNAEMKIVDPETGSSLP N  GEICIRGDQIMKGYLND E+T RTIDKEGWLHTGD
Sbjct: 370 GTVVRNAEMKIVDPETGSSLPRNHPGEICIRGDQIMKGYLNDPEATARTIDKEGWLHTGD 429

Query: 427 IGFIDDNNELFIIDRLKELIKFKTFQVAPAELEALLITHPKLRDAAVIGMPDVEAGEVPV 486
           IG+IDD++ELFI+DRLKELIK+K FQVAPAELEALL+THP + DAAV+ M D  AGEVPV
Sbjct: 430 IGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPCISDAAVVPMKDEAAGEVPV 489

Query: 487 AFVVEEKSGASATTTEEEVKEFIAKQVIFYKKLKRVFFVNAIPKAPSGKILRKEIRANLA 544
           AFVV+         TE+E+K+FI+KQVIFYK++ RVFFV AIPKAPSGKILRK++RA LA
Sbjct: 490 AFVVKSN---GHNITEDEIKQFISKQVIFYKRINRVFFVEAIPKAPSGKILRKDLRARLA 549

BLAST of CSPI07G19800 vs. ExPASy Swiss-Prot
Match: O24145 (4-coumarate--CoA ligase 1 OS=Nicotiana tabacum OX=4097 GN=4CL1 PE=2 SV=1)

HSP 1 Score: 760.8 bits (1963), Expect = 1.1e-218
Identity = 375/543 (69.06%), Postives = 444/543 (81.77%), Query Frame = 0

Query: 4   ESNQTNEFIFRSKLPNIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQLTA 63
           E+ Q+ + IFRSKLP+I+IP HLPLH Y F+N+S+F+SRPCLINGA   +YTY +V+LT 
Sbjct: 8   ETKQSGDLIFRSKLPDIYIPKHLPLHSYCFENISEFSSRPCLINGANDQIYTYAEVELTC 67

Query: 64  RRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKA 123
           R+VA GL+ LGI++ D +M LLPNSPEFVF F+GASY GAI T ANP +T  E+ KQAKA
Sbjct: 68  RKVAVGLNKLGIQQKDTIMILLPNSPEFVFAFMGASYLGAISTMANPLFTPAEVVKQAKA 127

Query: 124 ANAKLIVTMACFYDRVKD-LGENGVKIVCVDFSAEGCLHFSVLSGADESLMSRVDFSSDD 183
           ++AK+I+T +CF  +VKD   EN VK++C+D + EGCLHFS L+ +DE  +  V    DD
Sbjct: 128 SSAKIIITQSCFVGKVKDYASENDVKVICIDSAPEGCLHFSELTQSDEHEIPEVKIQPDD 187

Query: 184 VVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGQNPNLYYRGDDVILCVLPLFHIYSLN 243
           VVALPYSSGTTGLPKGVMLTHKGL+TSVAQQVDG+N NLY   +DV++CVLPLFHIYSLN
Sbjct: 188 VVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSEDVLMCVLPLFHIYSLN 247

Query: 244 SILLCGLRAGAAIMIMQKFDIVSLFQLIGKYKISIVPIVPPIFLAIAKSAEFEKYDVSSV 303
           SILLCGLR GAAI+IMQKFDI    +LI KYK+SI P VPPI LAIAKS   + YD+SSV
Sbjct: 248 SILLCGLRVGAAILIMQKFDIAPFLELIQKYKVSIGPFVPPIVLAIAKSPIVDSYDLSSV 307

Query: 304 RVLKSGGAPLGKELVEAVKAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVKSEACGT 363
           R + SG APLGKEL +AV+ KFP A L QGYGMTEAGPVL M LAFAKEPF +KS ACGT
Sbjct: 308 RTVMSGAAPLGKELEDAVRTKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGACGT 367

Query: 364 VVRNAEMKIVDPETGSSLPENSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGDIG 423
           VVRNAEMKIVDP+TG SLP N  GEICIRGDQIMKGYLND E+T RTIDKEGWLHTGDIG
Sbjct: 368 VVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATTRTIDKEGWLHTGDIG 427

Query: 424 FIDDNNELFIIDRLKELIKFKTFQVAPAELEALLITHPKLRDAAVIGMPDVEAGEVPVAF 483
           FID+++ELFI+DRLKELIK+K FQVAPAE+EALL+ HP + DAAV+ M D +AGEVPVAF
Sbjct: 428 FIDEDDELFIVDRLKELIKYKGFQVAPAEIEALLLNHPNISDAAVVPMKDEQAGEVPVAF 487

Query: 484 VVEEKSGASATTTEEEVKEFIAKQVIFYKKLKRVFFVNAIPKAPSGKILRKEIRANLASG 543
           VV     A    TE+EVK+FI+KQVIFYK++KRVFFV  +PK+PSGKILRK++RA LA+G
Sbjct: 488 VVRSNGSA---ITEDEVKDFISKQVIFYKRVKRVFFVETVPKSPSGKILRKDLRARLAAG 547

Query: 544 AYN 546
             N
Sbjct: 548 VPN 547

BLAST of CSPI07G19800 vs. ExPASy Swiss-Prot
Match: I3PB37 (4-coumarate:CoA ligase 1 OS=Petunia hybrida OX=4102 GN=4CL1 PE=1 SV=1)

HSP 1 Score: 759.2 bits (1959), Expect = 3.2e-218
Identity = 376/546 (68.86%), Postives = 448/546 (82.05%), Query Frame = 0

Query: 1   MAFESNQTNEFIFRSKLPNIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60
           M  E+NQ  + IFRSKLP+I+IP HLPLH Y F+N+S+F+SRPCLINGA   +YTY DV+
Sbjct: 3   METETNQ-GDLIFRSKLPDIYIPKHLPLHSYCFENISEFSSRPCLINGANNHIYTYADVE 62

Query: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LT+R+VAAGL+ LGI++ D +M LLPNSPEFVF F+GASY GAI T ANP +T  E+ KQ
Sbjct: 63  LTSRKVAAGLNKLGIQQKDTIMILLPNSPEFVFAFMGASYLGAISTMANPLFTPAEVVKQ 122

Query: 121 AKAANAKLIVTMACFYDRVKDLG-ENGVKIVCVDFSAEGCLHFSVLSGADESLMSRVDFS 180
           AKA+NAKLI+T ACF ++VKD   +N + ++C+D + EGC+HFS L+ ADE  +  V   
Sbjct: 123 AKASNAKLIITQACFVNKVKDYAFDNNLNVICIDSAPEGCIHFSELTQADEHDIPDVKIQ 182

Query: 181 SDDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGQNPNLYYRGDDVILCVLPLFHIY 240
           SDDVVALPYSSGTTGLPKGVMLTHKGL+TSVAQQVDG+N NLY   +DV++CVLPLFHIY
Sbjct: 183 SDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSEDVLMCVLPLFHIY 242

Query: 241 SLNSILLCGLRAGAAIMIMQKFDIVSLFQLIGKYKISIVPIVPPIFLAIAKSAEFEKYDV 300
           SLNS+LLCGLR GAAI+IMQKFDIV   +LI KYK++I P VPPI LAIAKS   + YD+
Sbjct: 243 SLNSVLLCGLRVGAAILIMQKFDIVQFCELIEKYKVTIGPFVPPIVLAIAKSPVVDNYDL 302

Query: 301 SSVRVLKSGGAPLGKELVEAVKAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVKSEA 360
           SSVR + SG APLGKEL +AV+ KFP A L QGYGMTEAGPVL M LAFAKEPF +KS A
Sbjct: 303 SSVRTVMSGAAPLGKELEDAVRIKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGA 362

Query: 361 CGTVVRNAEMKIVDPETGSSLPENSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTG 420
           CGTVVRNAEMKIVDP+TG SLP N  GEICIRGDQIMKGYLND  +T RTIDKEGWLHTG
Sbjct: 363 CGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPAATTRTIDKEGWLHTG 422

Query: 421 DIGFIDDNNELFIIDRLKELIKFKTFQVAPAELEALLITHPKLRDAAVIGMPDVEAGEVP 480
           DIG+ID+++ELFI+DRLKELIK+K FQVAPAELEALL+ HP + DAAV+ M D +AGEVP
Sbjct: 423 DIGYIDNDDELFIVDRLKELIKYKGFQVAPAELEALLLNHPNISDAAVVPMKDEQAGEVP 482

Query: 481 VAFVVEEKSGASATTTEEEVKEFIAKQVIFYKKLKRVFFVNAIPKAPSGKILRKEIRANL 540
           VAFVV       +  TE+EVK+F++KQVIFYK++KRVFFV  +PK+PSGKILRK++RA L
Sbjct: 483 VAFVVRSN---GSDITEDEVKDFVSKQVIFYKRIKRVFFVETVPKSPSGKILRKDLRARL 542

Query: 541 ASGAYN 546
           A+G  N
Sbjct: 543 AAGVPN 544

BLAST of CSPI07G19800 vs. ExPASy Swiss-Prot
Match: P14912 (4-coumarate--CoA ligase 1 OS=Petroselinum crispum OX=4043 GN=4CL1 PE=2 SV=1)

HSP 1 Score: 754.6 bits (1947), Expect = 7.8e-217
Identity = 370/534 (69.29%), Postives = 444/534 (83.15%), Query Frame = 0

Query: 10  EFIFRSKLPNIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQLTARRVAAG 69
           + IFRSKLP+I+IP HLPLH Y F+N+SK   + CLINGATG+ +TY  V+L +R+VA+G
Sbjct: 10  DLIFRSKLPDIYIPKHLPLHTYCFENISKVGDKSCLINGATGETFTYSQVELLSRKVASG 69

Query: 70  LHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKAANAKLI 129
           L+ LGI++GD +M LLPNSPE+ F FLGASYRGAI T ANPF+T+ E+ KQ KA+ AKLI
Sbjct: 70  LNKLGIQQGDTIMLLLPNSPEYFFAFLGASYRGAISTMANPFFTSAEVIKQLKASQAKLI 129

Query: 130 VTMACFYDRVKD-LGENGVKIVCVDFSAEGCLHFSVLSGADESLMSRVDFSSDDVVALPY 189
           +T AC+ D+VKD   E  ++I+C+D + + CLHFS L  ADES M  V  +SDDVVALPY
Sbjct: 130 ITQACYVDKVKDYAAEKNIQIICIDDAPQDCLHFSKLMEADESEMPEVVINSDDVVALPY 189

Query: 190 SSGTTGLPKGVMLTHKGLITSVAQQVDGQNPNLYYRGDDVILCVLPLFHIYSLNSILLCG 249
           SSGTTGLPKGVMLTHKGL+TSVAQQVDG NPNLY   +DV++C+LPLFHIYSLN++L CG
Sbjct: 190 SSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYMHSEDVMICILPLFHIYSLNAVLCCG 249

Query: 250 LRAGAAIMIMQKFDIVSLFQLIGKYKISIVPIVPPIFLAIAKSAEFEKYDVSSVRVLKSG 309
           LRAG  I+IMQKFDIV   +LI KYK++I P VPPI LAIAKS   +KYD+SSVR + SG
Sbjct: 250 LRAGVTILIMQKFDIVPFLELIQKYKVTIGPFVPPIVLAIAKSPVVDKYDLSSVRTVMSG 309

Query: 310 GAPLGKELVEAVKAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVKSEACGTVVRNAE 369
            APLGKEL +AV+AKFP A L QGYGMTEAGPVL M LAFAKEP+++KS ACGTVVRNAE
Sbjct: 310 AAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPYEIKSGACGTVVRNAE 369

Query: 370 MKIVDPETGSSLPENSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGDIGFIDDNN 429
           MKIVDPET +SLP N  GEICIRGDQIMKGYLND EST+ TID+EGWLHTGDIGFIDD++
Sbjct: 370 MKIVDPETNASLPRNQRGEICIRGDQIMKGYLNDPESTRTTIDEEGWLHTGDIGFIDDDD 429

Query: 430 ELFIIDRLKELIKFKTFQVAPAELEALLITHPKLRDAAVIGMPDVEAGEVPVAFVVEEKS 489
           ELFI+DRLKE+IK+K FQVAPAELEALL+THP + DAAV+ M D +AGEVPVAFVV    
Sbjct: 430 ELFIVDRLKEIIKYKGFQVAPAELEALLLTHPTISDAAVVPMIDEKAGEVPVAFVVRTN- 489

Query: 490 GASATTTEEEVKEFIAKQVIFYKKLKRVFFVNAIPKAPSGKILRKEIRANLASG 543
               TTTEEE+K+F++KQV+FYK++ RVFFV+AIPK+PSGKILRK++RA +ASG
Sbjct: 490 --GFTTTEEEIKQFVSKQVVFYKRIFRVFFVDAIPKSPSGKILRKDLRARIASG 540

BLAST of CSPI07G19800 vs. ExPASy TrEMBL
Match: A0A0A0K6Q5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G430230 PE=4 SV=1)

HSP 1 Score: 1062.4 bits (2746), Expect = 6.5e-307
Identity = 541/545 (99.27%), Postives = 543/545 (99.63%), Query Frame = 0

Query: 1   MAFESNQTNEFIFRSKLPNIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60
           MAFESNQTNEFIFRSKLP+IHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ
Sbjct: 1   MAFESNQTNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60

Query: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLGENGVKIVCVDFSAEGCLHFSVLSGADESLMSRVDFSS 180
           AKAANAKLIVTMACFYDRVKDLGENGVKIVCVDFSAEGCLHFSVLSGADESLMSRVDFSS
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLGENGVKIVCVDFSAEGCLHFSVLSGADESLMSRVDFSS 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGQNPNLYYRGDDVILCVLPLFHIYS 240
           DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGQNPNLYYRGDDVILCVLPLFHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGQNPNLYYRGDDVILCVLPLFHIYS 240

Query: 241 LNSILLCGLRAGAAIMIMQKFDIVSLFQLIGKYKISIVPIVPPIFLAIAKSAEFEKYDVS 300
           LNSILLCGLRAGAAIMIMQKFDIVSLFQLIGKYKISIVPIVPPIFLAIAKSAEFEKYDVS
Sbjct: 241 LNSILLCGLRAGAAIMIMQKFDIVSLFQLIGKYKISIVPIVPPIFLAIAKSAEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELVEAVKAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVKSEAC 360
           SVRVLKSGGAPLGKELVEAVKAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVK EAC
Sbjct: 301 SVRVLKSGGAPLGKELVEAVKAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVKFEAC 360

Query: 361 GTVVRNAEMKIVDPETGSSLPENSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKIVDPETG SLPENSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDPETGVSLPENSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420

Query: 421 IGFIDDNNELFIIDRLKELIKFKTFQVAPAELEALLITHPKLRDAAVIGMPDVEAGEVPV 480
           IGFIDDNNELFIIDRLKELIKFKTFQVAPAELEALLITHPKLRDAAVIGMPDVEAGEVPV
Sbjct: 421 IGFIDDNNELFIIDRLKELIKFKTFQVAPAELEALLITHPKLRDAAVIGMPDVEAGEVPV 480

Query: 481 AFVVEEKSGASATTTEEEVKEFIAKQVIFYKKLKRVFFVNAIPKAPSGKILRKEIRANLA 540
           AFVVEEKSGASATTTEEEVKEFIAKQVIFYK+LKRVFFVNAIPKAPSGKILRKEIRANLA
Sbjct: 481 AFVVEEKSGASATTTEEEVKEFIAKQVIFYKRLKRVFFVNAIPKAPSGKILRKEIRANLA 540

Query: 541 SGAYN 546
           SGAYN
Sbjct: 541 SGAYN 545

BLAST of CSPI07G19800 vs. ExPASy TrEMBL
Match: A0A5A7UU15 (4-coumarate--CoA ligase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold339G001480 PE=4 SV=1)

HSP 1 Score: 1022.3 bits (2642), Expect = 7.4e-295
Identity = 518/545 (95.05%), Postives = 531/545 (97.43%), Query Frame = 0

Query: 1   MAFESNQTNEFIFRSKLPNIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60
           MAFESNQT+EFIFRSKLP+IHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVY+Y+DV 
Sbjct: 1   MAFESNQTHEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYSYYDVN 60

Query: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTARRVAAGLH+LGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVAAGLHSLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLGENGVKIVCVDFSAEGCLHFSVLSGADESLMSRVDFSS 180
            KAANAKLIVTMACFYDRVKDL ENGVKIVCVDFS EGCLHFSVLSGADESLM RVDFSS
Sbjct: 121 VKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFSVEGCLHFSVLSGADESLMPRVDFSS 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGQNPNLYYRGDDVILCVLPLFHIYS 240
           DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGQNPNLYYRGDDVILCVLPLFHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGQNPNLYYRGDDVILCVLPLFHIYS 240

Query: 241 LNSILLCGLRAGAAIMIMQKFDIVSLFQLIGKYKISIVPIVPPIFLAIAKSAEFEKYDVS 300
           LNSILLCGLRAGAAI IMQKFDIVSLFQLIGKYK+SIVPIVPPIFLAIAKS EFEKYDVS
Sbjct: 241 LNSILLCGLRAGAAITIMQKFDIVSLFQLIGKYKVSIVPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELVEAVKAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVKSEAC 360
           SVRVLKSGGAPLGKELVEAVKAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVK+EAC
Sbjct: 301 SVRVLKSGGAPLGKELVEAVKAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVKAEAC 360

Query: 361 GTVVRNAEMKIVDPETGSSLPENSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKIVDPETG+SLPENS GEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDPETGASLPENSPGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420

Query: 421 IGFIDDNNELFIIDRLKELIKFKTFQVAPAELEALLITHPKLRDAAVIGMPDVEAGEVPV 480
           IGF+DDNNELFIIDRLKELIKFKTFQVAPAELEALLI HPKLRDAAVIGMPDVEAGEVPV
Sbjct: 421 IGFVDDNNELFIIDRLKELIKFKTFQVAPAELEALLIAHPKLRDAAVIGMPDVEAGEVPV 480

Query: 481 AFVVEEKSGASATTTEEEVKEFIAKQVIFYKKLKRVFFVNAIPKAPSGKILRKEIRANLA 540
           AFVVEEKSGA   TTEEEVK+FI KQV+FYK+LKRVFFVNAIPKAPSGKILRKE+RA LA
Sbjct: 481 AFVVEEKSGA---TTEEEVKQFITKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLA 540

Query: 541 SGAYN 546
           SGAYN
Sbjct: 541 SGAYN 542

BLAST of CSPI07G19800 vs. ExPASy TrEMBL
Match: A0A1S3CFE7 (4-coumarate--CoA ligase 2-like OS=Cucumis melo OX=3656 GN=LOC103499870 PE=4 SV=1)

HSP 1 Score: 1021.9 bits (2641), Expect = 9.7e-295
Identity = 517/545 (94.86%), Postives = 531/545 (97.43%), Query Frame = 0

Query: 1   MAFESNQTNEFIFRSKLPNIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60
           MAFESNQT+EFIFRSKLP+IHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVY+Y+DV 
Sbjct: 1   MAFESNQTHEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYSYYDVN 60

Query: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTARRVAAGLH+LGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVAAGLHSLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLGENGVKIVCVDFSAEGCLHFSVLSGADESLMSRVDFSS 180
            KAANAKLIVTMACFYDRVKDL ENGVKIVCVDFS EGCLHFSVLSGADESLM RVDFSS
Sbjct: 121 VKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFSVEGCLHFSVLSGADESLMPRVDFSS 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGQNPNLYYRGDDVILCVLPLFHIYS 240
           DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGQNPNLYYRGDDVILCVLPLFHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGQNPNLYYRGDDVILCVLPLFHIYS 240

Query: 241 LNSILLCGLRAGAAIMIMQKFDIVSLFQLIGKYKISIVPIVPPIFLAIAKSAEFEKYDVS 300
           LNSILLCGLRAGAAI IMQKFDIVSLFQLIGKYK+SIVPIVPPIFLAIAKS EFEKYDVS
Sbjct: 241 LNSILLCGLRAGAAITIMQKFDIVSLFQLIGKYKVSIVPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELVEAVKAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVKSEAC 360
           SVRVLKSGGAPLGKELVEAVKAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVK+EAC
Sbjct: 301 SVRVLKSGGAPLGKELVEAVKAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVKAEAC 360

Query: 361 GTVVRNAEMKIVDPETGSSLPENSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKIVDPETG+SLPENS GEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDPETGASLPENSPGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420

Query: 421 IGFIDDNNELFIIDRLKELIKFKTFQVAPAELEALLITHPKLRDAAVIGMPDVEAGEVPV 480
           IGF+DDNNELFIIDRLKELIKFKTFQVAPAELEALLI HPKLRDAAVIGMPDVEAGEVPV
Sbjct: 421 IGFVDDNNELFIIDRLKELIKFKTFQVAPAELEALLIAHPKLRDAAVIGMPDVEAGEVPV 480

Query: 481 AFVVEEKSGASATTTEEEVKEFIAKQVIFYKKLKRVFFVNAIPKAPSGKILRKEIRANLA 540
           AFVVEEKSGA   TTEEEVK+F+ KQV+FYK+LKRVFFVNAIPKAPSGKILRKE+RA LA
Sbjct: 481 AFVVEEKSGA---TTEEEVKQFVTKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLA 540

Query: 541 SGAYN 546
           SGAYN
Sbjct: 541 SGAYN 542

BLAST of CSPI07G19800 vs. ExPASy TrEMBL
Match: A0A5D3CH12 (4-coumarate--CoA ligase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G001340 PE=4 SV=1)

HSP 1 Score: 1020.8 bits (2638), Expect = 2.2e-294
Identity = 517/545 (94.86%), Postives = 530/545 (97.25%), Query Frame = 0

Query: 1   MAFESNQTNEFIFRSKLPNIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60
           MAFESNQT+EFIFRSKLP+IHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVY+Y+DV 
Sbjct: 1   MAFESNQTHEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYSYYDVN 60

Query: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTARRVAAGLH+LGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVAAGLHSLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLGENGVKIVCVDFSAEGCLHFSVLSGADESLMSRVDFSS 180
            KAANAKLIVTMACFYDRVKDL ENGVKIVCVDFS EGCLHFSVLSGADESLM RVDFSS
Sbjct: 121 VKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFSVEGCLHFSVLSGADESLMPRVDFSS 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGQNPNLYYRGDDVILCVLPLFHIYS 240
           DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGQNPNLYYRGDDVILCVLPLFHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGQNPNLYYRGDDVILCVLPLFHIYS 240

Query: 241 LNSILLCGLRAGAAIMIMQKFDIVSLFQLIGKYKISIVPIVPPIFLAIAKSAEFEKYDVS 300
           LNSILLCGLRAGAAI IMQKFDIVSLFQLIGKYK+SIVPIVPPIFLAIAKS EFEKYDVS
Sbjct: 241 LNSILLCGLRAGAAITIMQKFDIVSLFQLIGKYKVSIVPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELVEAVKAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVKSEAC 360
           SVRVLKSGGAPLGKELVEAVK KFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVK+EAC
Sbjct: 301 SVRVLKSGGAPLGKELVEAVKTKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVKAEAC 360

Query: 361 GTVVRNAEMKIVDPETGSSLPENSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKIVDPETG+SLPENS GEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDPETGASLPENSPGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420

Query: 421 IGFIDDNNELFIIDRLKELIKFKTFQVAPAELEALLITHPKLRDAAVIGMPDVEAGEVPV 480
           IGF+DDNNELFIIDRLKELIKFKTFQVAPAELEALLI HPKLRDAAVIGMPDVEAGEVPV
Sbjct: 421 IGFVDDNNELFIIDRLKELIKFKTFQVAPAELEALLIAHPKLRDAAVIGMPDVEAGEVPV 480

Query: 481 AFVVEEKSGASATTTEEEVKEFIAKQVIFYKKLKRVFFVNAIPKAPSGKILRKEIRANLA 540
           AFVVEEKSGA   TTEEEVK+FI KQV+FYK+LKRVFFVNAIPKAPSGKILRKE+RA LA
Sbjct: 481 AFVVEEKSGA---TTEEEVKQFITKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLA 540

Query: 541 SGAYN 546
           SGAYN
Sbjct: 541 SGAYN 542

BLAST of CSPI07G19800 vs. ExPASy TrEMBL
Match: A0A0A0K8H9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G430240 PE=4 SV=1)

HSP 1 Score: 969.5 bits (2505), Expect = 5.7e-279
Identity = 489/545 (89.72%), Postives = 516/545 (94.68%), Query Frame = 0

Query: 1   MAFESNQTNEFIFRSKLPNIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60
           MAFESN+TNEFIFRSKLP+IHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ
Sbjct: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60

Query: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLGENGVKIVCVDFSAEGCLHFSVLSGADESLMSRVDFSS 180
           AKAANAKLIVTMACFYDRVKDL ENGV+IVCVDF+ EGCLHFSVLSGADESL   VDFSS
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVQIVCVDFAVEGCLHFSVLSGADESLAPLVDFSS 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGQNPNLYYRGDDVILCVLPLFHIYS 240
           +DVVALPYSSGTTGLPKGVMLTHKGLITSVAQQ+DGQNPNLYY  +DVILCVLP FHIYS
Sbjct: 181 NDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGQNPNLYYHRNDVILCVLPFFHIYS 240

Query: 241 LNSILLCGLRAGAAIMIMQKFDIVSLFQLIGKYKISIVPIVPPIFLAIAKSAEFEKYDVS 300
           LNSILLCGLR GAAIMIMQKFDIV+L QLI K++ISI+PIVPPIFLAIAKS EFEKYDVS
Sbjct: 241 LNSILLCGLRVGAAIMIMQKFDIVALLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELVEAVKAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVKSEAC 360
           SVRVLKSGGAPLGKEL +AV+ KFP AIL QGYGMTEAGPVL+MSLAFAKEPFQVK+ AC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 360

Query: 361 GTVVRNAEMKIVDPETGSSLPENSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKIVD ETG+SLP NS+GEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420

Query: 421 IGFIDDNNELFIIDRLKELIKFKTFQVAPAELEALLITHPKLRDAAVIGMPDVEAGEVPV 480
           IGF+DD+NELFI+DRLKELIKFK FQVAPAELEALLITHPKL DAAVIGMPDVEAGEVPV
Sbjct: 421 IGFVDDDNELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480

Query: 481 AFVVEEKSGASATTTEEEVKEFIAKQVIFYKKLKRVFFVNAIPKAPSGKILRKEIRANLA 540
           AFV++   GA    +EEEVK+FIAKQV+FYK+LKRVFFVNAIPKAPSGKILRKE+RA LA
Sbjct: 481 AFVMKANGGA---ISEEEVKQFIAKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLA 540

Query: 541 SGAYN 546
           SGAYN
Sbjct: 541 SGAYN 542

BLAST of CSPI07G19800 vs. NCBI nr
Match: XP_004135895.1 (4-coumarate--CoA ligase 2 [Cucumis sativus] >KGN45188.1 hypothetical protein Csa_015658 [Cucumis sativus])

HSP 1 Score: 1062.4 bits (2746), Expect = 1.3e-306
Identity = 541/545 (99.27%), Postives = 543/545 (99.63%), Query Frame = 0

Query: 1   MAFESNQTNEFIFRSKLPNIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60
           MAFESNQTNEFIFRSKLP+IHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ
Sbjct: 1   MAFESNQTNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60

Query: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLGENGVKIVCVDFSAEGCLHFSVLSGADESLMSRVDFSS 180
           AKAANAKLIVTMACFYDRVKDLGENGVKIVCVDFSAEGCLHFSVLSGADESLMSRVDFSS
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLGENGVKIVCVDFSAEGCLHFSVLSGADESLMSRVDFSS 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGQNPNLYYRGDDVILCVLPLFHIYS 240
           DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGQNPNLYYRGDDVILCVLPLFHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGQNPNLYYRGDDVILCVLPLFHIYS 240

Query: 241 LNSILLCGLRAGAAIMIMQKFDIVSLFQLIGKYKISIVPIVPPIFLAIAKSAEFEKYDVS 300
           LNSILLCGLRAGAAIMIMQKFDIVSLFQLIGKYKISIVPIVPPIFLAIAKSAEFEKYDVS
Sbjct: 241 LNSILLCGLRAGAAIMIMQKFDIVSLFQLIGKYKISIVPIVPPIFLAIAKSAEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELVEAVKAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVKSEAC 360
           SVRVLKSGGAPLGKELVEAVKAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVK EAC
Sbjct: 301 SVRVLKSGGAPLGKELVEAVKAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVKFEAC 360

Query: 361 GTVVRNAEMKIVDPETGSSLPENSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKIVDPETG SLPENSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDPETGVSLPENSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420

Query: 421 IGFIDDNNELFIIDRLKELIKFKTFQVAPAELEALLITHPKLRDAAVIGMPDVEAGEVPV 480
           IGFIDDNNELFIIDRLKELIKFKTFQVAPAELEALLITHPKLRDAAVIGMPDVEAGEVPV
Sbjct: 421 IGFIDDNNELFIIDRLKELIKFKTFQVAPAELEALLITHPKLRDAAVIGMPDVEAGEVPV 480

Query: 481 AFVVEEKSGASATTTEEEVKEFIAKQVIFYKKLKRVFFVNAIPKAPSGKILRKEIRANLA 540
           AFVVEEKSGASATTTEEEVKEFIAKQVIFYK+LKRVFFVNAIPKAPSGKILRKEIRANLA
Sbjct: 481 AFVVEEKSGASATTTEEEVKEFIAKQVIFYKRLKRVFFVNAIPKAPSGKILRKEIRANLA 540

Query: 541 SGAYN 546
           SGAYN
Sbjct: 541 SGAYN 545

BLAST of CSPI07G19800 vs. NCBI nr
Match: KAA0058678.1 (4-coumarate--CoA ligase 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 1022.3 bits (2642), Expect = 1.5e-294
Identity = 518/545 (95.05%), Postives = 531/545 (97.43%), Query Frame = 0

Query: 1   MAFESNQTNEFIFRSKLPNIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60
           MAFESNQT+EFIFRSKLP+IHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVY+Y+DV 
Sbjct: 1   MAFESNQTHEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYSYYDVN 60

Query: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTARRVAAGLH+LGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVAAGLHSLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLGENGVKIVCVDFSAEGCLHFSVLSGADESLMSRVDFSS 180
            KAANAKLIVTMACFYDRVKDL ENGVKIVCVDFS EGCLHFSVLSGADESLM RVDFSS
Sbjct: 121 VKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFSVEGCLHFSVLSGADESLMPRVDFSS 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGQNPNLYYRGDDVILCVLPLFHIYS 240
           DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGQNPNLYYRGDDVILCVLPLFHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGQNPNLYYRGDDVILCVLPLFHIYS 240

Query: 241 LNSILLCGLRAGAAIMIMQKFDIVSLFQLIGKYKISIVPIVPPIFLAIAKSAEFEKYDVS 300
           LNSILLCGLRAGAAI IMQKFDIVSLFQLIGKYK+SIVPIVPPIFLAIAKS EFEKYDVS
Sbjct: 241 LNSILLCGLRAGAAITIMQKFDIVSLFQLIGKYKVSIVPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELVEAVKAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVKSEAC 360
           SVRVLKSGGAPLGKELVEAVKAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVK+EAC
Sbjct: 301 SVRVLKSGGAPLGKELVEAVKAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVKAEAC 360

Query: 361 GTVVRNAEMKIVDPETGSSLPENSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKIVDPETG+SLPENS GEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDPETGASLPENSPGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420

Query: 421 IGFIDDNNELFIIDRLKELIKFKTFQVAPAELEALLITHPKLRDAAVIGMPDVEAGEVPV 480
           IGF+DDNNELFIIDRLKELIKFKTFQVAPAELEALLI HPKLRDAAVIGMPDVEAGEVPV
Sbjct: 421 IGFVDDNNELFIIDRLKELIKFKTFQVAPAELEALLIAHPKLRDAAVIGMPDVEAGEVPV 480

Query: 481 AFVVEEKSGASATTTEEEVKEFIAKQVIFYKKLKRVFFVNAIPKAPSGKILRKEIRANLA 540
           AFVVEEKSGA   TTEEEVK+FI KQV+FYK+LKRVFFVNAIPKAPSGKILRKE+RA LA
Sbjct: 481 AFVVEEKSGA---TTEEEVKQFITKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLA 540

Query: 541 SGAYN 546
           SGAYN
Sbjct: 541 SGAYN 542

BLAST of CSPI07G19800 vs. NCBI nr
Match: XP_008461218.1 (PREDICTED: 4-coumarate--CoA ligase 2-like [Cucumis melo])

HSP 1 Score: 1021.9 bits (2641), Expect = 2.0e-294
Identity = 517/545 (94.86%), Postives = 531/545 (97.43%), Query Frame = 0

Query: 1   MAFESNQTNEFIFRSKLPNIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60
           MAFESNQT+EFIFRSKLP+IHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVY+Y+DV 
Sbjct: 1   MAFESNQTHEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYSYYDVN 60

Query: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTARRVAAGLH+LGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVAAGLHSLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLGENGVKIVCVDFSAEGCLHFSVLSGADESLMSRVDFSS 180
            KAANAKLIVTMACFYDRVKDL ENGVKIVCVDFS EGCLHFSVLSGADESLM RVDFSS
Sbjct: 121 VKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFSVEGCLHFSVLSGADESLMPRVDFSS 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGQNPNLYYRGDDVILCVLPLFHIYS 240
           DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGQNPNLYYRGDDVILCVLPLFHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGQNPNLYYRGDDVILCVLPLFHIYS 240

Query: 241 LNSILLCGLRAGAAIMIMQKFDIVSLFQLIGKYKISIVPIVPPIFLAIAKSAEFEKYDVS 300
           LNSILLCGLRAGAAI IMQKFDIVSLFQLIGKYK+SIVPIVPPIFLAIAKS EFEKYDVS
Sbjct: 241 LNSILLCGLRAGAAITIMQKFDIVSLFQLIGKYKVSIVPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELVEAVKAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVKSEAC 360
           SVRVLKSGGAPLGKELVEAVKAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVK+EAC
Sbjct: 301 SVRVLKSGGAPLGKELVEAVKAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVKAEAC 360

Query: 361 GTVVRNAEMKIVDPETGSSLPENSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKIVDPETG+SLPENS GEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDPETGASLPENSPGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420

Query: 421 IGFIDDNNELFIIDRLKELIKFKTFQVAPAELEALLITHPKLRDAAVIGMPDVEAGEVPV 480
           IGF+DDNNELFIIDRLKELIKFKTFQVAPAELEALLI HPKLRDAAVIGMPDVEAGEVPV
Sbjct: 421 IGFVDDNNELFIIDRLKELIKFKTFQVAPAELEALLIAHPKLRDAAVIGMPDVEAGEVPV 480

Query: 481 AFVVEEKSGASATTTEEEVKEFIAKQVIFYKKLKRVFFVNAIPKAPSGKILRKEIRANLA 540
           AFVVEEKSGA   TTEEEVK+F+ KQV+FYK+LKRVFFVNAIPKAPSGKILRKE+RA LA
Sbjct: 481 AFVVEEKSGA---TTEEEVKQFVTKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLA 540

Query: 541 SGAYN 546
           SGAYN
Sbjct: 541 SGAYN 542

BLAST of CSPI07G19800 vs. NCBI nr
Match: TYK10484.1 (4-coumarate--CoA ligase 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 1020.8 bits (2638), Expect = 4.4e-294
Identity = 517/545 (94.86%), Postives = 530/545 (97.25%), Query Frame = 0

Query: 1   MAFESNQTNEFIFRSKLPNIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60
           MAFESNQT+EFIFRSKLP+IHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVY+Y+DV 
Sbjct: 1   MAFESNQTHEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYSYYDVN 60

Query: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTARRVAAGLH+LGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVAAGLHSLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLGENGVKIVCVDFSAEGCLHFSVLSGADESLMSRVDFSS 180
            KAANAKLIVTMACFYDRVKDL ENGVKIVCVDFS EGCLHFSVLSGADESLM RVDFSS
Sbjct: 121 VKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFSVEGCLHFSVLSGADESLMPRVDFSS 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGQNPNLYYRGDDVILCVLPLFHIYS 240
           DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGQNPNLYYRGDDVILCVLPLFHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGQNPNLYYRGDDVILCVLPLFHIYS 240

Query: 241 LNSILLCGLRAGAAIMIMQKFDIVSLFQLIGKYKISIVPIVPPIFLAIAKSAEFEKYDVS 300
           LNSILLCGLRAGAAI IMQKFDIVSLFQLIGKYK+SIVPIVPPIFLAIAKS EFEKYDVS
Sbjct: 241 LNSILLCGLRAGAAITIMQKFDIVSLFQLIGKYKVSIVPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELVEAVKAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVKSEAC 360
           SVRVLKSGGAPLGKELVEAVK KFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVK+EAC
Sbjct: 301 SVRVLKSGGAPLGKELVEAVKTKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVKAEAC 360

Query: 361 GTVVRNAEMKIVDPETGSSLPENSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKIVDPETG+SLPENS GEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDPETGASLPENSPGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420

Query: 421 IGFIDDNNELFIIDRLKELIKFKTFQVAPAELEALLITHPKLRDAAVIGMPDVEAGEVPV 480
           IGF+DDNNELFIIDRLKELIKFKTFQVAPAELEALLI HPKLRDAAVIGMPDVEAGEVPV
Sbjct: 421 IGFVDDNNELFIIDRLKELIKFKTFQVAPAELEALLIAHPKLRDAAVIGMPDVEAGEVPV 480

Query: 481 AFVVEEKSGASATTTEEEVKEFIAKQVIFYKKLKRVFFVNAIPKAPSGKILRKEIRANLA 540
           AFVVEEKSGA   TTEEEVK+FI KQV+FYK+LKRVFFVNAIPKAPSGKILRKE+RA LA
Sbjct: 481 AFVVEEKSGA---TTEEEVKQFITKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLA 540

Query: 541 SGAYN 546
           SGAYN
Sbjct: 541 SGAYN 542

BLAST of CSPI07G19800 vs. NCBI nr
Match: XP_038897161.1 (4-coumarate--CoA ligase 2-like [Benincasa hispida])

HSP 1 Score: 972.6 bits (2513), Expect = 1.4e-279
Identity = 493/545 (90.46%), Postives = 515/545 (94.50%), Query Frame = 0

Query: 1   MAFESNQTNEFIFRSKLPNIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60
           MA ESNQTNEFIFRSKLP+IHIPNHLPLHDY+FQNL KFASRPCLINGATGDVY++HDVQ
Sbjct: 1   MALESNQTNEFIFRSKLPDIHIPNHLPLHDYIFQNLPKFASRPCLINGATGDVYSFHDVQ 60

Query: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANAKLIVTMACFYDRVKDLGENGVKIVCVDFSAEGCLHFSVLSGADESLMSRVDFSS 180
           AKAANAKLIVTMACFYDRVKDL ENGVKIVCVDF+ EGCLHFSVLSGADE+    VDFS 
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFAVEGCLHFSVLSGADEAHAPAVDFSP 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGQNPNLYYRGDDVILCVLPLFHIYS 240
           DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQ+DGQNPNLYYRGDDVILCVLP FHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYRGDDVILCVLPFFHIYS 240

Query: 241 LNSILLCGLRAGAAIMIMQKFDIVSLFQLIGKYKISIVPIVPPIFLAIAKSAEFEKYDVS 300
           LNSILLCG+RAGAAIMIMQKFDIVSLFQLI KY+ISI+PIVPPIFLAIAKS+EFEKYDVS
Sbjct: 241 LNSILLCGIRAGAAIMIMQKFDIVSLFQLIEKYRISIMPIVPPIFLAIAKSSEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELVEAVKAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVKSEAC 360
           SVRVLKSGGAPLGKEL +AV+AKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVK+ AC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVRAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVKAGAC 360

Query: 361 GTVVRNAEMKIVDPETGSSLPENSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKIVDPETG+SLP NSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420

Query: 421 IGFIDDNNELFIIDRLKELIKFKTFQVAPAELEALLITHPKLRDAAVIGMPDVEAGEVPV 480
           IGFIDD +ELFI+DRLKELIKFK FQVAPAELEALLITHPKL DAAVIGM D EAGEVPV
Sbjct: 421 IGFIDD-DELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMADEEAGEVPV 480

Query: 481 AFVVEEKSGASATTTEEEVKEFIAKQVIFYKKLKRVFFVNAIPKAPSGKILRKEIRANLA 540
           AFVV+E  G   T TEEEVK FI KQV+FYK+LK V+FVNAIPKAPSGKILRKE+R  LA
Sbjct: 481 AFVVKESDG---TITEEEVKHFITKQVVFYKRLKSVYFVNAIPKAPSGKILRKELRTKLA 540

Query: 541 SGAYN 546
           SGAYN
Sbjct: 541 SGAYN 541

BLAST of CSPI07G19800 vs. TAIR 10
Match: AT3G21240.1 (4-coumarate:CoA ligase 2 )

HSP 1 Score: 731.1 bits (1886), Expect = 6.6e-211
Identity = 361/542 (66.61%), Postives = 448/542 (82.66%), Query Frame = 0

Query: 8   TNEFIFRSKLPNIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQLTARRVA 67
           +N+ IFRS+LP+I+IPNHLPLHDY+F+N+S+FA++PCLING TG+VYTY DV +T+R++A
Sbjct: 18  SNDVIFRSRLPDIYIPNHLPLHDYIFENISEFAAKPCLINGPTGEVYTYADVHVTSRKLA 77

Query: 68  AGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKAANAK 127
           AGLHNLG+K+ DVVM LLPNSPE V TFL AS+ GAI T+ANPF+T  EI+KQAKA+ AK
Sbjct: 78  AGLHNLGVKQHDVVMILLPNSPEVVLTFLAASFIGAITTSANPFFTPAEISKQAKASAAK 137

Query: 128 LIVTMACFYDRVKDLGENGVKIVCVDFSA--EGCLHFSVLSGADESLMSRV--DFSSDDV 187
           LIVT + + D++K+L  +GV IV  D  A  E CL FS L+ ++E  +  +    S +DV
Sbjct: 138 LIVTQSRYVDKIKNLQNDGVLIVTTDSDAIPENCLRFSELTQSEEPRVDSIPEKISPEDV 197

Query: 188 VALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGQNPNLYYRGDDVILCVLPLFHIYSLNS 247
           VALP+SSGTTGLPKGVMLTHKGL+TSVAQQVDG+NPNLY+  DDVILCVLP+FHIY+LNS
Sbjct: 198 VALPFSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFNRDDVILCVLPMFHIYALNS 257

Query: 248 ILLCGLRAGAAIMIMQKFDIVSLFQLIGKYKISIVPIVPPIFLAIAKSAEFEKYDVSSVR 307
           I+LC LR GA I+IM KF+I  L + I + K+++  +VPPI LAIAKS E EKYD+SSVR
Sbjct: 258 IMLCSLRVGATILIMPKFEITLLLEQIQRCKVTVAMVVPPIVLAIAKSPETEKYDLSSVR 317

Query: 308 VLKSGGAPLGKELVEAVKAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVKSEACGTV 367
           ++KSG APLGKEL +A+ AKFP A L QGYGMTEAGPVL MSL FAKEPF VKS ACGTV
Sbjct: 318 MVKSGAAPLGKELEDAISAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKSGACGTV 377

Query: 368 VRNAEMKIVDPETGSSLPENSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGDIGF 427
           VRNAEMKI+DP+TG SLP N  GEICIRG+QIMKGYLND  +T  TIDK+GWLHTGD+GF
Sbjct: 378 VRNAEMKILDPDTGDSLPRNKPGEICIRGNQIMKGYLNDPLATASTIDKDGWLHTGDVGF 437

Query: 428 IDDNNELFIIDRLKELIKFKTFQVAPAELEALLITHPKLRDAAVIGMPDVEAGEVPVAFV 487
           IDD++ELFI+DRLKELIK+K FQVAPAELE+LLI HP++ D AV+ M + +AGEVPVAFV
Sbjct: 438 IDDDDELFIVDRLKELIKYKGFQVAPAELESLLIGHPEINDVAVVAMKEEDAGEVPVAFV 497

Query: 488 VEEKSGASATTTEEEVKEFIAKQVIFYKKLKRVFFVNAIPKAPSGKILRKEIRANLASGA 546
           V  K    +  +E+E+K+F++KQV+FYK++ +VFF ++IPKAPSGKILRK++RA LA+G 
Sbjct: 498 VRSK---DSNISEDEIKQFVSKQVVFYKRINKVFFTDSIPKAPSGKILRKDLRARLANGL 556

BLAST of CSPI07G19800 vs. TAIR 10
Match: AT1G51680.1 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 727.6 bits (1877), Expect = 7.2e-210
Identity = 364/547 (66.54%), Postives = 444/547 (81.17%), Query Frame = 0

Query: 5   SNQTNEFIFRSKLPNIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQLTAR 64
           +N  ++ IFRSKLP+I+IPNHL LHDY+FQN+S+FA++PCLING TG VYTY DV + +R
Sbjct: 17  NNNNSDVIFRSKLPDIYIPNHLSLHDYIFQNISEFATKPCLINGPTGHVYTYSDVHVISR 76

Query: 65  RVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKAA 124
           ++AA  H LG+ + DVVM LLPN PEFV +FL AS+RGA  TAANPF+T  EIAKQAKA+
Sbjct: 77  QIAANFHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKAS 136

Query: 125 NAKLIVTMACFYDRVKDL-GENGVKIVCVDFS-----AEGCLHFSVLSGAD---ESLMSR 184
           N KLI+T A + D++K L  ++GV IVC+D +      EGCL F+ L+ +      ++  
Sbjct: 137 NTKLIITEARYVDKIKPLQNDDGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDS 196

Query: 185 VDFSSDDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGQNPNLYYRGDDVILCVLPL 244
           V+ S DDVVALPYSSGTTGLPKGVMLTHKGL+TSVAQQVDG+NPNLY+  DDVILCVLP+
Sbjct: 197 VEISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPM 256

Query: 245 FHIYSLNSILLCGLRAGAAIMIMQKFDIVSLFQLIGKYKISIVPIVPPIFLAIAKSAEFE 304
           FHIY+LNSI+LCGLR GAAI+IM KF+I  L +LI + K+++ P+VPPI LAIAKS+E E
Sbjct: 257 FHIYALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETE 316

Query: 305 KYDVSSVRVLKSGGAPLGKELVEAVKAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQV 364
           KYD+SS+RV+KSG APLGKEL +AV AKFP A L QGYGMTEAGPVL MSL FAKEPF V
Sbjct: 317 KYDLSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPV 376

Query: 365 KSEACGTVVRNAEMKIVDPETGSSLPENSAGEICIRGDQIMKGYLNDLESTKRTIDKEGW 424
           KS ACGTVVRNAEMKIVDP+TG SL  N  GEICIRG QIMKGYLN+  +T  TIDK+GW
Sbjct: 377 KSGACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDGW 436

Query: 425 LHTGDIGFIDDNNELFIIDRLKELIKFKTFQVAPAELEALLITHPKLRDAAVIGMPDVEA 484
           LHTGDIG IDD++ELFI+DRLKELIK+K FQVAPAELEALLI HP + D AV+ M +  A
Sbjct: 437 LHTGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEEAA 496

Query: 485 GEVPVAFVVEEKSGASATTTEEEVKEFIAKQVIFYKKLKRVFFVNAIPKAPSGKILRKEI 543
           GEVPVAFVV+ K    +  +E++VK+F++KQV+FYK++ +VFF  +IPKAPSGKILRK++
Sbjct: 497 GEVPVAFVVKSK---DSELSEDDVKQFVSKQVVFYKRINKVFFTESIPKAPSGKILRKDL 556

BLAST of CSPI07G19800 vs. TAIR 10
Match: AT1G51680.3 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 676.8 bits (1745), Expect = 1.5e-194
Identity = 341/512 (66.60%), Postives = 413/512 (80.66%), Query Frame = 0

Query: 5   SNQTNEFIFRSKLPNIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQLTAR 64
           +N  ++ IFRSKLP+I+IPNHL LHDY+FQN+S+FA++PCLING TG VYTY DV + +R
Sbjct: 17  NNNNSDVIFRSKLPDIYIPNHLSLHDYIFQNISEFATKPCLINGPTGHVYTYSDVHVISR 76

Query: 65  RVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKAA 124
           ++AA  H LG+ + DVVM LLPN PEFV +FL AS+RGA  TAANPF+T  EIAKQAKA+
Sbjct: 77  QIAANFHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKAS 136

Query: 125 NAKLIVTMACFYDRVKDL-GENGVKIVCVDFS-----AEGCLHFSVLSGAD---ESLMSR 184
           N KLI+T A + D++K L  ++GV IVC+D +      EGCL F+ L+ +      ++  
Sbjct: 137 NTKLIITEARYVDKIKPLQNDDGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDS 196

Query: 185 VDFSSDDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGQNPNLYYRGDDVILCVLPL 244
           V+ S DDVVALPYSSGTTGLPKGVMLTHKGL+TSVAQQVDG+NPNLY+  DDVILCVLP+
Sbjct: 197 VEISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPM 256

Query: 245 FHIYSLNSILLCGLRAGAAIMIMQKFDIVSLFQLIGKYKISIVPIVPPIFLAIAKSAEFE 304
           FHIY+LNSI+LCGLR GAAI+IM KF+I  L +LI + K+++ P+VPPI LAIAKS+E E
Sbjct: 257 FHIYALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETE 316

Query: 305 KYDVSSVRVLKSGGAPLGKELVEAVKAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQV 364
           KYD+SS+RV+KSG APLGKEL +AV AKFP A L QGYGMTEAGPVL MSL FAKEPF V
Sbjct: 317 KYDLSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPV 376

Query: 365 KSEACGTVVRNAEMKIVDPETGSSLPENSAGEICIRGDQIMKGYLNDLESTKRTIDKEGW 424
           KS ACGTVVRNAEMKIVDP+TG SL  N  GEICIRG QIMKGYLN+  +T  TIDK+GW
Sbjct: 377 KSGACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDGW 436

Query: 425 LHTGDIGFIDDNNELFIIDRLKELIKFKTFQVAPAELEALLITHPKLRDAAVIGMPDVEA 484
           LHTGDIG IDD++ELFI+DRLKELIK+K FQVAPAELEALLI HP + D AV+ M +  A
Sbjct: 437 LHTGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEEAA 496

Query: 485 GEVPVAFVVEEKSGASATTTEEEVKEFIAKQV 508
           GEVPVAFVV+ K    +  +E++VK+F++KQV
Sbjct: 497 GEVPVAFVVKSK---DSELSEDDVKQFVSKQV 525

BLAST of CSPI07G19800 vs. TAIR 10
Match: AT3G21230.1 (4-coumarate:CoA ligase 5 )

HSP 1 Score: 654.1 bits (1686), Expect = 1.0e-187
Identity = 325/549 (59.20%), Postives = 420/549 (76.50%), Query Frame = 0

Query: 4   ESNQTNEFIFRSKLPNIHIPNHLPLHDYVFQNLS----KFASRPCLINGATGDVYTYHDV 63
           E   +++FIFRSKLP+I IPNHLPL DYVFQ  S      +S  C+I+GATG + TY DV
Sbjct: 20  EEEPSHDFIFRSKLPDIFIPNHLPLTDYVFQRFSGDGDGDSSTTCIIDGATGRILTYADV 79

Query: 64  QLTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAK 123
           Q   RR+AAG+H LGI+ GDVVM LLPNSPEF  +FL  +Y GA+ T ANPFYT  EIAK
Sbjct: 80  QTNMRRIAAGIHRLGIRHGDVVMLLLPNSPEFALSFLAVAYLGAVSTTANPFYTQPEIAK 139

Query: 124 QAKAANAKLIVTMACFYDRVKDLGENGVKIVCVD---------FSAEGCLHFSVLSGADE 183
           QAKA+ AK+I+T  C  D++ +L  +GV IVC+D          S +GC+ F+ L+ ADE
Sbjct: 140 QAKASAAKMIITKKCLVDKLTNLKNDGVLIVCLDDDGDNGVVSSSDDGCVSFTELTQADE 199

Query: 184 SLMSRVDFSSDDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGQNPNLYYRGDDVIL 243
           + + +   S +D VA+PYSSGTTGLPKGVM+THKGL+TS+AQ+VDG+NPNL +  +DVIL
Sbjct: 200 TELLKPKISPEDTVAMPYSSGTTGLPKGVMITHKGLVTSIAQKVDGENPNLNFTANDVIL 259

Query: 244 CVLPLFHIYSLNSILLCGLRAGAAIMIMQKFDIVSLFQLIGKYKISIVPIVPPIFLAIAK 303
           C LP+FHIY+L++++L  +R GAA++I+ +F++  + +LI +YK+++VP+ PP+ LA  K
Sbjct: 260 CFLPMFHIYALDALMLSAMRTGAALLIVPRFELNLVMELIQRYKVTVVPVAPPVVLAFIK 319

Query: 304 SAEFEKYDVSSVRVLKSGGAPLGKELVEAVKAKFPAAILAQGYGMTEAGPVLTMSLAFAK 363
           S E E+YD+SSVR++ SG A L KEL +AV+ KFP AI  QGYGMTE+G V   SLAFAK
Sbjct: 320 SPETERYDLSSVRIMLSGAATLKKELEDAVRLKFPNAIFGQGYGMTESGTV-AKSLAFAK 379

Query: 364 EPFQVKSEACGTVVRNAEMKIVDPETGSSLPENSAGEICIRGDQIMKGYLNDLESTKRTI 423
            PF+ KS ACGTV+RNAEMK+VD ETG SLP N +GEIC+RG Q+MKGYLND E+T RTI
Sbjct: 380 NPFKTKSGACGTVIRNAEMKVVDTETGISLPRNKSGEICVRGHQLMKGYLNDPEATARTI 439

Query: 424 DKEGWLHTGDIGFIDDNNELFIIDRLKELIKFKTFQVAPAELEALLITHPKLRDAAVIGM 483
           DK+GWLHTGDIGF+DD++E+FI+DRLKELIKFK +QVAPAELEALLI+HP + DAAV+ M
Sbjct: 440 DKDGWLHTGDIGFVDDDDEIFIVDRLKELIKFKGYQVAPAELEALLISHPSIDDAAVVAM 499

Query: 484 PDVEAGEVPVAFVVEEKSGASATTTEEEVKEFIAKQVIFYKKLKRVFFVNAIPKAPSGKI 540
            D  A EVPVAFV   +    +  TE++VK ++ KQV+ YK++K VFF+  IPKA SGKI
Sbjct: 500 KDEVADEVPVAFVARSQ---GSQLTEDDVKSYVNKQVVHYKRIKMVFFIEVIPKAVSGKI 559

BLAST of CSPI07G19800 vs. TAIR 10
Match: AT1G51680.2 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 646.0 bits (1665), Expect = 2.8e-185
Identity = 322/473 (68.08%), Postives = 385/473 (81.40%), Query Frame = 0

Query: 5   SNQTNEFIFRSKLPNIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQLTAR 64
           +N  ++ IFRSKLP+I+IPNHL LHDY+FQN+S+FA++PCLING TG VYTY DV + +R
Sbjct: 17  NNNNSDVIFRSKLPDIYIPNHLSLHDYIFQNISEFATKPCLINGPTGHVYTYSDVHVISR 76

Query: 65  RVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKAA 124
           ++AA  H LG+ + DVVM LLPN PEFV +FL AS+RGA  TAANPF+T  EIAKQAKA+
Sbjct: 77  QIAANFHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKAS 136

Query: 125 NAKLIVTMACFYDRVKDL-GENGVKIVCVDFS-----AEGCLHFSVLSGAD---ESLMSR 184
           N KLI+T A + D++K L  ++GV IVC+D +      EGCL F+ L+ +      ++  
Sbjct: 137 NTKLIITEARYVDKIKPLQNDDGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDS 196

Query: 185 VDFSSDDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGQNPNLYYRGDDVILCVLPL 244
           V+ S DDVVALPYSSGTTGLPKGVMLTHKGL+TSVAQQVDG+NPNLY+  DDVILCVLP+
Sbjct: 197 VEISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPM 256

Query: 245 FHIYSLNSILLCGLRAGAAIMIMQKFDIVSLFQLIGKYKISIVPIVPPIFLAIAKSAEFE 304
           FHIY+LNSI+LCGLR GAAI+IM KF+I  L +LI + K+++ P+VPPI LAIAKS+E E
Sbjct: 257 FHIYALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETE 316

Query: 305 KYDVSSVRVLKSGGAPLGKELVEAVKAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQV 364
           KYD+SS+RV+KSG APLGKEL +AV AKFP A L QGYGMTEAGPVL MSL FAKEPF V
Sbjct: 317 KYDLSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPV 376

Query: 365 KSEACGTVVRNAEMKIVDPETGSSLPENSAGEICIRGDQIMKGYLNDLESTKRTIDKEGW 424
           KS ACGTVVRNAEMKIVDP+TG SL  N  GEICIRG QIMKGYLN+  +T  TIDK+GW
Sbjct: 377 KSGACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDGW 436

Query: 425 LHTGDIGFIDDNNELFIIDRLKELIKFKTFQVAPAELEALLITHPKLRDAAVI 469
           LHTGDIG IDD++ELFI+DRLKELIK+K FQVAPAELEALLI HP + D AV+
Sbjct: 437 LHTGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVV 489

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O241462.3e-22170.954-coumarate--CoA ligase 2 OS=Nicotiana tabacum OX=4097 GN=4CL2 PE=1 SV=1[more]
O245407.5e-22070.724-coumarate--CoA ligase OS=Vanilla planifolia OX=51239 GN=4CL PE=3 SV=1[more]
O241451.1e-21869.064-coumarate--CoA ligase 1 OS=Nicotiana tabacum OX=4097 GN=4CL1 PE=2 SV=1[more]
I3PB373.2e-21868.864-coumarate:CoA ligase 1 OS=Petunia hybrida OX=4102 GN=4CL1 PE=1 SV=1[more]
P149127.8e-21769.294-coumarate--CoA ligase 1 OS=Petroselinum crispum OX=4043 GN=4CL1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0K6Q56.5e-30799.27Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G430230 PE=4 SV=1[more]
A0A5A7UU157.4e-29595.054-coumarate--CoA ligase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_s... [more]
A0A1S3CFE79.7e-29594.864-coumarate--CoA ligase 2-like OS=Cucumis melo OX=3656 GN=LOC103499870 PE=4 SV=1[more]
A0A5D3CH122.2e-29494.864-coumarate--CoA ligase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
A0A0A0K8H95.7e-27989.72Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G430240 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_004135895.11.3e-30699.274-coumarate--CoA ligase 2 [Cucumis sativus] >KGN45188.1 hypothetical protein Csa... [more]
KAA0058678.11.5e-29495.054-coumarate--CoA ligase 2-like [Cucumis melo var. makuwa][more]
XP_008461218.12.0e-29494.86PREDICTED: 4-coumarate--CoA ligase 2-like [Cucumis melo][more]
TYK10484.14.4e-29494.864-coumarate--CoA ligase 2-like [Cucumis melo var. makuwa][more]
XP_038897161.11.4e-27990.464-coumarate--CoA ligase 2-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT3G21240.16.6e-21166.614-coumarate:CoA ligase 2 [more]
AT1G51680.17.2e-21066.544-coumarate:CoA ligase 1 [more]
AT1G51680.31.5e-19466.604-coumarate:CoA ligase 1 [more]
AT3G21230.11.0e-18759.204-coumarate:CoA ligase 5 [more]
AT1G51680.22.8e-18568.084-coumarate:CoA ligase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000873AMP-dependent synthetase/ligasePFAMPF00501AMP-bindingcoord: 35..442
e-value: 2.2E-103
score: 346.1
NoneNo IPR availableGENE3D3.30.300.30coord: 437..541
e-value: 2.9E-28
score: 100.5
NoneNo IPR availablePANTHERPTHR24096LONG-CHAIN-FATTY-ACID--COA LIGASEcoord: 7..540
NoneNo IPR availablePANTHERPTHR24096:SF3824-COUMARATE--COA LIGASE 2-LIKEcoord: 7..540
NoneNo IPR availableCDDcd059044CLcoord: 22..534
e-value: 0.0
score: 719.403
NoneNo IPR availableSUPERFAMILY56801Acetyl-CoA synthetase-likecoord: 12..540
IPR025110AMP-binding enzyme, C-terminal domainPFAMPF13193AMP-binding_Ccoord: 451..529
e-value: 1.3E-16
score: 61.3
IPR042099ANL, N-terminal domainGENE3D3.40.50.12780coord: 3..436
e-value: 3.3E-162
score: 542.0
IPR020845AMP-binding, conserved sitePROSITEPS00455AMP_BINDINGcoord: 186..197

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI07G19800.1CSPI07G19800.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016405 CoA-ligase activity