CSPI07G18810 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI07G18810
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionCytochrome P450 protein
LocationChr7: 16711458 .. 16713754 (-)
RNA-Seq ExpressionCSPI07G18810
SyntenyCSPI07G18810
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATTCTGGAAACCTCTCCTCCTCCTCCTTCTCCCCTCCAATTTCCATCGGACACTTCGGGCTCCGGTCGCTCCTCGATGTTCAAATCTTAGAGATCACTTTCGCCCTTTTCGTGTTCTTGGTTATTCATGTTGTTAGACAGAAGAGACGACACGGCTTGCCTATATGGCCAATCGTTGGAATGTTACCAGCTTTGATTACAGCAACACGTGGGAATCTCTACGAGTGGATTACCGACCTCTTGTGTCATCACAAGGGCACATTCCAATTTCGAGGTCCATGGCTTACTAGTCTCAACTGTGTTGTCACTGCTGATCCTCGAAACTTAGAACACCTACTCAAGACAAAATTTTCTTCCTACCCAAAAGGGTCGTTCTTTCGCGACACCGTGAGAGACTTACTTGGTGATGGAATATTCGGGGCTGACGACGAGACATGGCAACGACAGAGAAAGGTAGCCAGCATTGAGTTTCACTCGGCAAGGTTTCGACAAATGACAGTGGAGTCATTGGTGGAGCTTGTGCACTCGAGGCTGGAAGCAGTGTTGGAGGATTTCGAGGGGCAATCAACAACGGTGGACTTGCAGGATGTTCTATTGAGGTTGACGTTTGATAATGTGTGTATGATTGCCTTTGGGGTGGATCCCGGTTGCTTGCGCAGCGGCTTGCCGGAGATTCCATTTGCTAAGGCCTTTGAGGATGCAACTGAAGTCACTGTTATACGCTTCATCACACCTACATTCATATGGAAATTCATGAGGTATTTTCTTTTTCTAATTTTGCATATTCAAATAGTTAATCCTTTTTTGTTTGAAAATAGTTTATTGTAGAGCTGCCCACCAAAACCTAACTAATCAGTTAAGATATTACTAGTAATCAATGTCAATAAAATTTTGACTCCATACTTCTACAGCAACAAAGTATTGATTAAATTGTTAGAAAAAGGGCTAAATTACAAGCTTTGGTAAATTATAACCATAGCTAAATTACCATTGAACTATGCTAAGCTATTACTTTCAAATTTTGTAACATTACCTACAACTTTTTAAAAAATACTCTCAGAATAGATGTCATTTAAGAACATCCCCAATGATCATTTTCTTTTCCTAAGTCATTGTTGCACCTTTTTCAAACCACAAATTAAAAAAATATTTCTTATAATTTAGGGTGGAAAAAGCCTATCATTTAGTTTTGAGACAACAAAGTGTAAATAGATTATTTTGAGTGTAATGTGTAATTTCATAAATATATATCTATGTATCAAATCGTTATTCAGGTATTATTTTTATTTAAAAAAAACCCAATTTTTTTTAATTAATTTAAATTTAAGCAGGTACCTGAACATAGGAATTGAAAAGAAGCTGAAGCAATCGATCAAAGCCGTCGACGAATTTGCGGAGGACGTGATTCAAACACGGAAGAGAGAACTCTCTACGCCGGCGGAATCTCAAGCTGAAAACCAAAGATCCGACCTATTAACTGTATTCATGAAGCTAAGGGACGAGCAAGGGCGCCCATTCTCCGATAAGTTTCTCAGAGATATTTGCGTCAATTTCATACTCGCCGGAAGAGACACGTCTTCCGTAGCATTGAGTTGGTTCTTCTTGTTGCTCGATCAAAATCCCGATGTAGAAGATAAAATTCTAGAAGAAATTTGCAGAATCGTCGGCGAACGAGAAGAGGCCAAAACCAGAGAGCTTCGATTCGATTCATTAGTGTTCAAACCAGAGGAGATAAAAAAAATGGAGTATTTGCACGCCGCCATTTCCGAAGCCCTAAGATTGTATCCATCAGTTCCATTCGATCACAAAGAGGTAATTATAACAATTACTCATTCACATTCTAATTTCGAAAGAAAACTTCAGAAATCTAATCGATAATCCGTCTGATTCAGGTTGTGGAGGACGATGTATTCCCAGACGGAACGGTTCTGAAGAAAGGAACGAAAATCATCTACGCGATATACGCAATGGGAAGAATGGAAGCGATTTGGGGGAAAGATTGTCGCGAATTCAAACCTGAGAGATGGATGAGAGAGGGTCGATTCATGAGCGAATCGGCATATCGATTCACCGCCTTCAATGGGGGCCCTCGGCTGTGTCTTGGAAAGGATTTTGCGTATTACCAAATGAAATACGTGGCCGCTTCTATCATCAGCCGGTACAGGGTAGCGGTGGTGAGGGGGCATCCGGTGGAGCCGAAGCTTGCCCTAACTTTCTACATGAAGAAGGGCTTGAAGGTCAATTTGATCAAACGAGAAGAGGGGCAACTTCGGAAATACATTAAGTTAAATTGA

mRNA sequence

ATGGATTCTGGAAACCTCTCCTCCTCCTCCTTCTCCCCTCCAATTTCCATCGGACACTTCGGGCTCCGGTCGCTCCTCGATGTTCAAATCTTAGAGATCACTTTCGCCCTTTTCGTGTTCTTGGTTATTCATGTTGTTAGACAGAAGAGACGACACGGCTTGCCTATATGGCCAATCGTTGGAATGTTACCAGCTTTGATTACAGCAACACGTGGGAATCTCTACGAGTGGATTACCGACCTCTTGTGTCATCACAAGGGCACATTCCAATTTCGAGGTCCATGGCTTACTAGTCTCAACTGTGTTGTCACTGCTGATCCTCGAAACTTAGAACACCTACTCAAGACAAAATTTTCTTCCTACCCAAAAGGGTCGTTCTTTCGCGACACCGTGAGAGACTTACTTGGTGATGGAATATTCGGGGCTGACGACGAGACATGGCAACGACAGAGAAAGGTAGCCAGCATTGAGTTTCACTCGGCAAGGTTTCGACAAATGACAGTGGAGTCATTGGTGGAGCTTGTGCACTCGAGGCTGGAAGCAGTGTTGGAGGATTTCGAGGGGCAATCAACAACGGTGGACTTGCAGGATGTTCTATTGAGGTTGACGTTTGATAATGTGTGTATGATTGCCTTTGGGGTGGATCCCGGTTGCTTGCGCAGCGGCTTGCCGGAGATTCCATTTGCTAAGGCCTTTGAGGATGCAACTGAAGTCACTGTTATACGCTTCATCACACCTACATTCATATGGAAATTCATGAGGTACCTGAACATAGGAATTGAAAAGAAGCTGAAGCAATCGATCAAAGCCGTCGACGAATTTGCGGAGGACGTGATTCAAACACGGAAGAGAGAACTCTCTACGCCGGCGGAATCTCAAGCTGAAAACCAAAGATCCGACCTATTAACTGTATTCATGAAGCTAAGGGACGAGCAAGGGCGCCCATTCTCCGATAAGTTTCTCAGAGATATTTGCGTCAATTTCATACTCGCCGGAAGAGACACGTCTTCCGTAGCATTGAGTTGGTTCTTCTTGTTGCTCGATCAAAATCCCGATGTAGAAGATAAAATTCTAGAAGAAATTTGCAGAATCGTCGGCGAACGAGAAGAGGCCAAAACCAGAGAGCTTCGATTCGATTCATTAGTGTTCAAACCAGAGGAGATAAAAAAAATGGAGTATTTGCACGCCGCCATTTCCGAAGCCCTAAGATTGTATCCATCAGTTCCATTCGATCACAAAGAGGTTGTGGAGGACGATGTATTCCCAGACGGAACGGTTCTGAAGAAAGGAACGAAAATCATCTACGCGATATACGCAATGGGAAGAATGGAAGCGATTTGGGGGAAAGATTGTCGCGAATTCAAACCTGAGAGATGGATGAGAGAGGGTCGATTCATGAGCGAATCGGCATATCGATTCACCGCCTTCAATGGGGGCCCTCGGCTGTGTCTTGGAAAGGATTTTGCGTATTACCAAATGAAATACGTGGCCGCTTCTATCATCAGCCGGTACAGGGTAGCGGTGGTGAGGGGGCATCCGGTGGAGCCGAAGCTTGCCCTAACTTTCTACATGAAGAAGGGCTTGAAGGTCAATTTGATCAAACGAGAAGAGGGGCAACTTCGGAAATACATTAAGTTAAATTGA

Coding sequence (CDS)

ATGGATTCTGGAAACCTCTCCTCCTCCTCCTTCTCCCCTCCAATTTCCATCGGACACTTCGGGCTCCGGTCGCTCCTCGATGTTCAAATCTTAGAGATCACTTTCGCCCTTTTCGTGTTCTTGGTTATTCATGTTGTTAGACAGAAGAGACGACACGGCTTGCCTATATGGCCAATCGTTGGAATGTTACCAGCTTTGATTACAGCAACACGTGGGAATCTCTACGAGTGGATTACCGACCTCTTGTGTCATCACAAGGGCACATTCCAATTTCGAGGTCCATGGCTTACTAGTCTCAACTGTGTTGTCACTGCTGATCCTCGAAACTTAGAACACCTACTCAAGACAAAATTTTCTTCCTACCCAAAAGGGTCGTTCTTTCGCGACACCGTGAGAGACTTACTTGGTGATGGAATATTCGGGGCTGACGACGAGACATGGCAACGACAGAGAAAGGTAGCCAGCATTGAGTTTCACTCGGCAAGGTTTCGACAAATGACAGTGGAGTCATTGGTGGAGCTTGTGCACTCGAGGCTGGAAGCAGTGTTGGAGGATTTCGAGGGGCAATCAACAACGGTGGACTTGCAGGATGTTCTATTGAGGTTGACGTTTGATAATGTGTGTATGATTGCCTTTGGGGTGGATCCCGGTTGCTTGCGCAGCGGCTTGCCGGAGATTCCATTTGCTAAGGCCTTTGAGGATGCAACTGAAGTCACTGTTATACGCTTCATCACACCTACATTCATATGGAAATTCATGAGGTACCTGAACATAGGAATTGAAAAGAAGCTGAAGCAATCGATCAAAGCCGTCGACGAATTTGCGGAGGACGTGATTCAAACACGGAAGAGAGAACTCTCTACGCCGGCGGAATCTCAAGCTGAAAACCAAAGATCCGACCTATTAACTGTATTCATGAAGCTAAGGGACGAGCAAGGGCGCCCATTCTCCGATAAGTTTCTCAGAGATATTTGCGTCAATTTCATACTCGCCGGAAGAGACACGTCTTCCGTAGCATTGAGTTGGTTCTTCTTGTTGCTCGATCAAAATCCCGATGTAGAAGATAAAATTCTAGAAGAAATTTGCAGAATCGTCGGCGAACGAGAAGAGGCCAAAACCAGAGAGCTTCGATTCGATTCATTAGTGTTCAAACCAGAGGAGATAAAAAAAATGGAGTATTTGCACGCCGCCATTTCCGAAGCCCTAAGATTGTATCCATCAGTTCCATTCGATCACAAAGAGGTTGTGGAGGACGATGTATTCCCAGACGGAACGGTTCTGAAGAAAGGAACGAAAATCATCTACGCGATATACGCAATGGGAAGAATGGAAGCGATTTGGGGGAAAGATTGTCGCGAATTCAAACCTGAGAGATGGATGAGAGAGGGTCGATTCATGAGCGAATCGGCATATCGATTCACCGCCTTCAATGGGGGCCCTCGGCTGTGTCTTGGAAAGGATTTTGCGTATTACCAAATGAAATACGTGGCCGCTTCTATCATCAGCCGGTACAGGGTAGCGGTGGTGAGGGGGCATCCGGTGGAGCCGAAGCTTGCCCTAACTTTCTACATGAAGAAGGGCTTGAAGGTCAATTTGATCAAACGAGAAGAGGGGCAACTTCGGAAATACATTAAGTTAAATTGA

Protein sequence

MDSGNLSSSSFSPPISIGHFGLRSLLDVQILEITFALFVFLVIHVVRQKRRHGLPIWPIVGMLPALITATRGNLYEWITDLLCHHKGTFQFRGPWLTSLNCVVTADPRNLEHLLKTKFSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVHSRLEAVLEDFEGQSTTVDLQDVLLRLTFDNVCMIAFGVDPGCLRSGLPEIPFAKAFEDATEVTVIRFITPTFIWKFMRYLNIGIEKKLKQSIKAVDEFAEDVIQTRKRELSTPAESQAENQRSDLLTVFMKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWFFLLLDQNPDVEDKILEEICRIVGEREEAKTRELRFDSLVFKPEEIKKMEYLHAAISEALRLYPSVPFDHKEVVEDDVFPDGTVLKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMREGRFMSESAYRFTAFNGGPRLCLGKDFAYYQMKYVAASIISRYRVAVVRGHPVEPKLALTFYMKKGLKVNLIKREEGQLRKYIKLN*
Homology
BLAST of CSPI07G18810 vs. ExPASy Swiss-Prot
Match: Q9FMY1 (Cytochrome P450 86B1 OS=Arabidopsis thaliana OX=3702 GN=CYP86B1 PE=2 SV=1)

HSP 1 Score: 767.3 bits (1980), Expect = 1.2e-220
Identity = 376/537 (70.02%), Postives = 439/537 (81.75%), Query Frame = 0

Query: 7   SSSSFSPPISIGHFGLRSLLDVQILEITFALFVFLVIHVVRQKRRHGLPIWPIVGMLPAL 66
           SSSS  P +S   F LR   DVQILE+  A+FVF+ IH +RQK+  GLP+WP +GMLP+L
Sbjct: 18  SSSSSDPLVSRRLFLLR---DVQILELLIAIFVFVAIHALRQKKYQGLPVWPFLGMLPSL 77

Query: 67  ITATRGNLYEWITDLLCHHKGTFQFRGPWLTSLNCVVTADPRNLEHLLKTKFSSYPKGSF 126
               RGN+YEW++D+LC   GTFQFRGPW +SLN  +T DPRN+EHLLK +FS +PKGS+
Sbjct: 78  AFGLRGNIYEWLSDVLCLQNGTFQFRGPWFSSLNSTITCDPRNVEHLLKNRFSVFPKGSY 137

Query: 127 FRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVHSRLEAVLEDF 186
           FRD +RDLLGDGIF ADDETWQRQRK ASIEFHSA+FRQ+T +SL ELVH RL  VLE  
Sbjct: 138 FRDNLRDLLGDGIFNADDETWQRQRKTASIEFHSAKFRQLTTQSLFELVHKRLLPVLETS 197

Query: 187 EGQSTTVDLQDVLLRLTFDNVCMIAFGVDPGCLRSGLPEIPFAKAFEDATEVTVIRFITP 246
              S+ +DLQDVLLRLTFDNVCMIAFGVDPGCL    P IPFAKAFEDATE  V+RF+ P
Sbjct: 198 VKSSSPIDLQDVLLRLTFDNVCMIAFGVDPGCLGPDQPVIPFAKAFEDATEAAVVRFVMP 257

Query: 247 TFIWKFMRYLNIGIEKKLKQSIKAVDEFAEDVIQTRKRELSTPAESQAENQRSDLLTVFM 306
           T +WKFMRYL+IG EKKLK+SIK VD+FA++VI+TRK+ELS   E+    +RSDLLTVFM
Sbjct: 258 TCVWKFMRYLDIGTEKKLKESIKGVDDFADEVIRTRKKELSLEGET---TKRSDLLTVFM 317

Query: 307 KLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWFFLLLDQNPDVEDKILEEICRIVG 366
            LRDE+G  FSDKFLRDICVNFILAGRDTSSVALSWFF LL++NP+VE+KI+ E+C+I+ 
Sbjct: 318 GLRDEKGESFSDKFLRDICVNFILAGRDTSSVALSWFFWLLEKNPEVEEKIMVEMCKILR 377

Query: 367 EREEAKTRELRFDSLVFKPEEIKKMEYLHAAISEALRLYPSVPFDHKEVVEDDVFPDGTV 426
           +R++    E      VF PEEIKKM+YL AA+SEALRLYPSVP DHKEV EDDVFPDGT+
Sbjct: 378 QRDDHGNAEKSDYEPVFGPEEIKKMDYLQAALSEALRLYPSVPVDHKEVQEDDVFPDGTM 437

Query: 427 LKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMREGRFMSESAYRFTAFNGGPRLCLGK 486
           LKKG K+IYAIYAMGRMEAIWGKDC EF+PERW+R+GRFMSESAY+FTAFNGGPRLCLGK
Sbjct: 438 LKKGDKVIYAIYAMGRMEAIWGKDCLEFRPERWLRDGRFMSESAYKFTAFNGGPRLCLGK 497

Query: 487 DFAYYQMKYVAASIISRYRVAVVRGHPVEPKLALTFYMKKGLKVNLIKREEGQLRKY 544
           DFAYYQMK  AA+I+ RY+V VV GH VEPKLALT YMK GL VNLI R   ++ +Y
Sbjct: 498 DFAYYQMKSTAAAIVYRYKVKVVNGHKVEPKLALTMYMKHGLMVNLINRSVSEIDQY 548

BLAST of CSPI07G18810 vs. ExPASy Swiss-Prot
Match: Q9CAD6 (Cytochrome P450 86A7 OS=Arabidopsis thaliana OX=3702 GN=CYP86A7 PE=2 SV=1)

HSP 1 Score: 458.0 bits (1177), Expect = 1.5e-127
Identity = 243/517 (47.00%), Postives = 336/517 (64.99%), Query Frame = 0

Query: 26  LDVQILEITFALFVFLVIHVVRQKRRH-GLPIWPIVGMLPALITATRGNLYEWITDLLCH 85
           +D     I   L V  +I  V  +R + G  +WP+VG LPALIT     ++++I D L  
Sbjct: 1   MDGSTAAIILTLIVTYIIWFVSLRRSYKGPRVWPLVGSLPALITNAH-RMHDFIADNLRM 60

Query: 86  HKGTFQ---FRGPWLTSL--NCVVTADPRNLEHLLKTKFSSYPKGSFFRDTVRDLLGDGI 145
             GT+Q   F  P+L     +  VT DP+NLEH+LKT+F +YPKG  ++    DLLGDGI
Sbjct: 61  CGGTYQTCIFPIPFLAKKQGHVTVTCDPKNLEHILKTRFDNYPKGPSWQSVFHDLLGDGI 120

Query: 146 FGADDETWQRQRKVASIEFHSARFRQMTVESLVELVHSRLEAVLEDFEGQSTTVDLQDVL 205
           F +D +TW+ QRK A++EF +   RQ     +   + +RL  +LE    ++  +DLQDVL
Sbjct: 121 FNSDGDTWRFQRKTAALEFTTRTLRQAMARWVDRAIKNRLVPILESARSRAEPIDLQDVL 180

Query: 206 LRLTFDNVCMIAFGVDPGCLRSGLPEIPFAKAFEDATEVTVIRFITPTFIWKFMRYLNIG 265
           LRLTFDN+C + FG DP  L    PE  FA AF+ ATE T+ RFI P FIWK  ++L +G
Sbjct: 181 LRLTFDNICGLTFGKDPRTLSPEFPENGFAVAFDGATEATLQRFIMPEFIWKIRKWLRLG 240

Query: 266 IEKKLKQSIKAVDEFAEDVIQTRKRELSTPAESQAENQRSDLLTVFMKLRDEQGRPFSDK 325
           +E  + +SI  VD +  ++I TRK EL    + Q E++  DLL+ FMK ++     +SDK
Sbjct: 241 LEDDMSRSISHVDNYLSEIINTRKLELL--GQQQDESRHDDLLSRFMKKKES----YSDK 300

Query: 326 FLRDICVNFILAGRDTSSVALSWFFLLLDQNPDVEDKILEEICRIVGEREEAKTRELRFD 385
           +L+ + +NFILAGRDTSSVA+SWFF L+  NP VE+KI+ EIC I+ +  +    +   +
Sbjct: 301 YLKYVALNFILAGRDTSSVAMSWFFWLVSLNPRVEEKIINEICTILIKTRDTNVSKWTDE 360

Query: 386 SLVFKPEEIKKMEYLHAAISEALRLYPSVPFDHKEVVEDDVFPDGTVLKKGTKIIYAIYA 445
            L F  +EI ++ YL AA+SE LRLYPSVP D K VV +DV PDGT +  G+ + Y+IY+
Sbjct: 361 PLTF--DEIDQLVYLKAALSETLRLYPSVPEDSKFVVANDVLPDGTFVPSGSNVTYSIYS 420

Query: 446 MGRMEAIWGKDCREFKPERWMREGRFMSESAYRFTAFNGGPRLCLGKDFAYYQMKYVAAS 505
           +GRM+ IWG+DC EFKPERW+ E R    + Y+F AFN GPR+CLGKD AY QMK + AS
Sbjct: 421 VGRMKFIWGEDCLEFKPERWLEESRDEKCNQYKFVAFNAGPRICLGKDLAYLQMKSITAS 480

Query: 506 IISRYRVAVVRGHPVEPKLALTFYMKKGLKVNLIKRE 537
           I+ R+R+ V  GH VE K++LT +MK GLK+++ KR+
Sbjct: 481 ILLRHRLTVAPGHRVEQKMSLTLFMKFGLKMDVHKRD 508

BLAST of CSPI07G18810 vs. ExPASy Swiss-Prot
Match: B3RFJ6 (Cytochrome P450 86A22 OS=Petunia hybrida OX=4102 GN=CYP86A22 PE=1 SV=1)

HSP 1 Score: 451.4 bits (1160), Expect = 1.4e-125
Identity = 247/516 (47.87%), Postives = 326/516 (63.18%), Query Frame = 0

Query: 28  VQILEITFALFVFLVIHVVRQKRRHGLPIWPIVGMLPALITATRGN-LYEWITDLLCHHK 87
           + I+ I  A  ++        K   G  +WP+VG LP LI    GN ++EWI D L    
Sbjct: 7   MMIVAIVAAYLLWFKSITKSMKGPKGPTMWPVVGSLPGLI--ENGNRMHEWIADNLRACS 66

Query: 88  GTFQ---FRGPWLTSLNCV-VTADPRNLEHLLKTKFSSYPKGSFFRDTVRDLLGDGIFGA 147
           GT+Q      P+L     V VT DP+NLEH+LK +F +YPKG  ++    DLLG+GIF +
Sbjct: 67  GTYQTCICAIPFLARKQLVTVTCDPKNLEHILKVRFDNYPKGPTWQAVFHDLLGEGIFNS 126

Query: 148 DDETWQRQRKVASIEFHSARFRQMTVESLVELVHSRLEAVLEDFEGQSTTVDLQDVLLRL 207
           D +TW  QRK A++EF +   RQ     +   + +R   +LE  + Q   VDLQD+LLRL
Sbjct: 127 DGDTWLFQRKTAALEFTTRTLRQAMGRWVNRAIKNRFCPILEMAQVQGKPVDLQDLLLRL 186

Query: 208 TFDNVCMIAFGVDPGCLRSGLPEIPFAKAFEDATEVTVIRFITPTFIWKFMRYLNIGIEK 267
           TFDN+C +AFG DP  L   LPE  FA +F+ ATE T+ RFI P F+WK  + L +G+E 
Sbjct: 187 TFDNICGLAFGKDPETLSPELPENNFATSFDRATEATLHRFIMPEFVWKLKKMLGLGLEV 246

Query: 268 KLKQSIKAVDEFAEDVIQTRKRELSTPAESQAENQRSDLLTVFMKLRDEQGRPFSDKFLR 327
            L  S+K VD +  DVI TRK EL          Q  DLL+ FMK ++     +SDKFL+
Sbjct: 247 SLNNSLKQVDNYMTDVINTRKLELLN--HQNGGPQHDDLLSRFMKKKES----YSDKFLQ 306

Query: 328 DICVNFILAGRDTSSVALSWFFLLLDQNPDVEDKILEEICRIVGEREEAKTRELRFDSLV 387
            + +NFILAGRDTSSVALSWFF L+  NP VE+KIL EIC I+ E     T +   + LV
Sbjct: 307 HVALNFILAGRDTSSVALSWFFWLVSSNPRVEEKILVEICTILAETRGNDTSKWLEEPLV 366

Query: 388 FKPEEIKKMEYLHAAISEALRLYPSVPFDHKEVVEDDVFPDGTVLKKGTKIIYAIYAMGR 447
           F  EE+ ++ YL AA+SE LRLYPSVP D K V+ DD  PDGT +  G+ I Y+IY+ GR
Sbjct: 367 F--EEVDQLMYLKAALSETLRLYPSVPEDSKHVISDDYLPDGTFVPAGSNITYSIYSTGR 426

Query: 448 MEAIWGKDCREFKPERWMRE--GRFMSESAYRFTAFNGGPRLCLGKDFAYYQMKYVAASI 507
           M+ IWG+DC EFKPERWM +   +F  +  +RF AFN GPR+CLGKD AY QMK +AA++
Sbjct: 427 MKFIWGEDCLEFKPERWMSQDGDKFQVQDTFRFVAFNAGPRICLGKDLAYLQMKSIAAAV 486

Query: 508 ISRYRVAVVRGHPVEPKLALTFYMKKGLKVNLIKRE 537
           + R+R+AV  GH VE K++LT +MK GL +N+  R+
Sbjct: 487 LLRHRLAVAPGHKVEQKMSLTLFMKDGLVMNVTPRD 512

BLAST of CSPI07G18810 vs. ExPASy Swiss-Prot
Match: Q9LMM1 (Cytochrome P450 86A4 OS=Arabidopsis thaliana OX=3702 GN=CYP86A4 PE=1 SV=1)

HSP 1 Score: 448.0 bits (1151), Expect = 1.6e-124
Identity = 237/492 (48.17%), Postives = 318/492 (64.63%), Query Frame = 0

Query: 53  GLPIWPIVGMLPALITATRGNLYEWITDLLCHHKGTFQ---FRGPWLTSLN--CVVTADP 112
           G  +WP++G LP LI   R  ++EWIT+ L    GT+Q   F  P+L        VT DP
Sbjct: 29  GPRVWPLLGSLPGLI-EQRDRMHEWITENLRACGGTYQTCIFAVPFLAKKQGLVTVTCDP 88

Query: 113 RNLEHLLKTKFSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMT 172
           +NLEH+LKT+F +YPKG  ++    DLLG GIF +D +TW  QRK A++EF +   RQ  
Sbjct: 89  KNLEHMLKTRFDNYPKGPTWQSVFHDLLGQGIFNSDGDTWLFQRKTAALEFTTRTLRQAM 148

Query: 173 VESLVELVHSRLEAVLEDFEGQSTTVDLQDVLLRLTFDNVCMIAFGVDPGCLRSGLPEIP 232
              +   +  R   +L   +  +  VDLQD++LRLTFDN+C +AFG D      GLPE  
Sbjct: 149 GRWVNRGIKLRFCPILATAQDNAEPVDLQDLILRLTFDNICGLAFGKDTRTCAPGLPENG 208

Query: 233 FAKAFEDATEVTVIRFITPTFIWKFMRYLNIGIEKKLKQSIKAVDEFAEDVIQTRKRELS 292
           FA AF+ ATE ++ RFI P F+WK  ++L +G+E  L +S+  +DE+   VI TRK+EL 
Sbjct: 209 FASAFDRATEASLQRFIIPKFMWKLKKWLGLGLEVSLSRSLGEIDEYLAAVINTRKQELM 268

Query: 293 TPAESQAENQRSDLLTVFMKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWFFLLL 352
           +  ES    +  DLL+ FM  + E    +SD FL+ + +NFILAGRDTSSVALSWFF L+
Sbjct: 269 SQQESGTHQRHDDLLSRFMMKKTES---YSDTFLQHVALNFILAGRDTSSVALSWFFWLI 328

Query: 353 DQNPDVEDKILEEICRIVGE-REEAKTRELRFDSLVFKPEEIKKMEYLHAAISEALRLYP 412
             +P VEDKI+ EIC ++ E R          + L F  +EI ++ YL AAISE LRLYP
Sbjct: 329 TMHPTVEDKIVREICSVLIETRGTDDVASWTEEPLGF--DEIDRLVYLKAAISETLRLYP 388

Query: 413 SVPFDHKEVVEDDVFPDGTVLKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMR--EGR 472
           SVP D K V  DDV PDGT +  G+ + Y+IYA GRM++ WG+DC EF PERW+   +G+
Sbjct: 389 SVPEDSKHVENDDVLPDGTFVPAGSSVTYSIYAAGRMKSTWGEDCLEFNPERWISPIDGK 448

Query: 473 FMSESAYRFTAFNGGPRLCLGKDFAYYQMKYVAASIISRYRVAVVRGHPVEPKLALTFYM 532
           F++   YRF AFN GPR+CLGKD AY QMK +AA+++ R+R+ VV GH VE K++LT +M
Sbjct: 449 FINHDQYRFVAFNAGPRICLGKDLAYLQMKTIAAAVLLRHRLTVVPGHKVEQKMSLTLFM 508

Query: 533 KKGLKVNLIKRE 537
           K GL VNL KR+
Sbjct: 509 KNGLLVNLYKRD 514

BLAST of CSPI07G18810 vs. ExPASy Swiss-Prot
Match: O23066 (Cytochrome P450 86A2 OS=Arabidopsis thaliana OX=3702 GN=CYP86A2 PE=1 SV=1)

HSP 1 Score: 443.0 bits (1138), Expect = 5.0e-123
Identity = 229/491 (46.64%), Postives = 319/491 (64.97%), Query Frame = 0

Query: 53  GLPIWPIVGMLPALITATRGNLYEWITDLLCHHKGTFQ---FRGPWLTSLN--CVVTADP 112
           G  +WP++G LP LI   R  +++WIT+ L    GT+Q      P+L        VT DP
Sbjct: 29  GPRVWPVLGSLPGLI-EQRDRMHDWITENLRACGGTYQTCICAVPFLAKKQGLVTVTCDP 88

Query: 113 RNLEHLLKTKFSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMT 172
           +N+EH+LKT+F +YPKG  ++    D LG GIF +D +TW  QRK A++EF +   RQ  
Sbjct: 89  KNIEHMLKTRFDNYPKGPTWQAVFHDFLGQGIFNSDGDTWLFQRKTAALEFTTRTLRQAM 148

Query: 173 VESLVELVHSRLEAVLEDFEGQSTTVDLQDVLLRLTFDNVCMIAFGVDPGCLRSGLPEIP 232
              +   +  R   +LE  +     VDLQD++LRLTFDN+C +AFG D      GLPE  
Sbjct: 149 GRWVNRGIKLRFCPILETAQNNYEPVDLQDLILRLTFDNICGLAFGKDTRTCAPGLPENG 208

Query: 233 FAKAFEDATEVTVIRFITPTFIWKFMRYLNIGIEKKLKQSIKAVDEFAEDVIQTRKRELS 292
           FA AF+ ATE ++ RFI P F+W+  ++L +G+E  L +S+  +D + + VI TRK+EL 
Sbjct: 209 FASAFDRATEASLQRFILPEFLWRLKKWLGLGLEVSLSRSLGEIDGYLDAVINTRKQELL 268

Query: 293 TPAESQAENQRSDLLTVFMKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWFFLLL 352
           +  ES  + +  DLL+ FMK +D+    +S+ FLR + +NFILAGRDTSSVALSWFF L+
Sbjct: 269 SQRESGVQ-RHDDLLSRFMKKKDQS---YSETFLRHVALNFILAGRDTSSVALSWFFWLI 328

Query: 353 DQNPDVEDKILEEICRIVGEREEAKTRELRFDSLVFKPEEIKKMEYLHAAISEALRLYPS 412
             +P VEDKI+ EIC ++ E           + L F  +E+ ++ YL AA+SE LRLYPS
Sbjct: 329 TTHPTVEDKIVREICSVLIETRGTDVSSWTAEPLEF--DEVDRLVYLKAALSETLRLYPS 388

Query: 413 VPFDHKEVVEDDVFPDGTVLKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMR--EGRF 472
           VP D K VV DD+ PDGT +  G+ + Y+IYA GRM++ WG+DC EFKPERW+   +G+F
Sbjct: 389 VPEDSKHVVNDDILPDGTFVPAGSSVTYSIYAAGRMKSTWGEDCLEFKPERWISPDDGKF 448

Query: 473 MSESAYRFTAFNGGPRLCLGKDFAYYQMKYVAASIISRYRVAVVRGHPVEPKLALTFYMK 532
           ++   YRF AFN GPR+CLGKD AY QMK +AA+++ R+R+ V  GH VE K++LT +MK
Sbjct: 449 VNHDQYRFVAFNAGPRICLGKDLAYLQMKTIAAAVLLRHRLTVAPGHKVEQKMSLTLFMK 508

Query: 533 KGLKVNLIKRE 537
            GL VN+ KR+
Sbjct: 509 NGLLVNVHKRD 512

BLAST of CSPI07G18810 vs. ExPASy TrEMBL
Match: A0A0A0K879 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G420770 PE=3 SV=1)

HSP 1 Score: 1086.6 bits (2809), Expect = 0.0e+00
Identity = 544/551 (98.73%), Postives = 546/551 (99.09%), Query Frame = 0

Query: 1   MDSGNL----SSSSFSPPISIGHFGLRSLLDVQILEITFALFVFLVIHVVRQKRRHGLPI 60
           MDSGNL    SSSSFSPPISIGHFGLRSLLDVQILEITFALFVFLVIHVVRQKRRHGLPI
Sbjct: 1   MDSGNLSSSSSSSSFSPPISIGHFGLRSLLDVQILEITFALFVFLVIHVVRQKRRHGLPI 60

Query: 61  WPIVGMLPALITATRGNLYEWITDLLCHHKGTFQFRGPWLTSLNCVVTADPRNLEHLLKT 120
           WPIVGMLPALITATRGNLYEWITDLLC HKGTFQFRGPWLTSLNCVVTADPRNLEHLLKT
Sbjct: 61  WPIVGMLPALITATRGNLYEWITDLLCRHKGTFQFRGPWLTSLNCVVTADPRNLEHLLKT 120

Query: 121 KFSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVH 180
           KFSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVH
Sbjct: 121 KFSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVH 180

Query: 181 SRLEAVLEDFEGQSTTVDLQDVLLRLTFDNVCMIAFGVDPGCLRSGLPEIPFAKAFEDAT 240
           SRLEAVLEDFEGQ+TTVDLQDVLLRLTFDNVCMIAFGVDPGCLRSGLPEIPFAKAFEDAT
Sbjct: 181 SRLEAVLEDFEGQATTVDLQDVLLRLTFDNVCMIAFGVDPGCLRSGLPEIPFAKAFEDAT 240

Query: 241 EVTVIRFITPTFIWKFMRYLNIGIEKKLKQSIKAVDEFAEDVIQTRKRELSTPAESQAEN 300
           EVTVIRFITPTFIWKFMRYLNIGIEKKLKQSIKAVDEFAEDVIQTRKRELSTPAESQAEN
Sbjct: 241 EVTVIRFITPTFIWKFMRYLNIGIEKKLKQSIKAVDEFAEDVIQTRKRELSTPAESQAEN 300

Query: 301 QRSDLLTVFMKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWFFLLLDQNPDVEDK 360
           QRSDLLTVFMKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWFFLLLDQNPDVEDK
Sbjct: 301 QRSDLLTVFMKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWFFLLLDQNPDVEDK 360

Query: 361 ILEEICRIVGEREEAKTRELRFDSLVFKPEEIKKMEYLHAAISEALRLYPSVPFDHKEVV 420
           ILEEICRIVGEREEAKTRELRFDSLVFKPEEIK+MEYLHAAISEALRLYPSVPFDHKEVV
Sbjct: 361 ILEEICRIVGEREEAKTRELRFDSLVFKPEEIKRMEYLHAAISEALRLYPSVPFDHKEVV 420

Query: 421 EDDVFPDGTVLKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMREGRFMSESAYRFTAF 480
           EDDVFPDGTVLKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMREGRFMSESAYRFTAF
Sbjct: 421 EDDVFPDGTVLKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMREGRFMSESAYRFTAF 480

Query: 481 NGGPRLCLGKDFAYYQMKYVAASIISRYRVAVVRGHPVEPKLALTFYMKKGLKVNLIKRE 540
           NGGPRLCLGKDFAYYQMKYVAASIISRYRVAVVRGHPVEPKLALTFYMKKGLKVNLIKRE
Sbjct: 481 NGGPRLCLGKDFAYYQMKYVAASIISRYRVAVVRGHPVEPKLALTFYMKKGLKVNLIKRE 540

Query: 541 EGQLRKYIKLN 548
           EGQLRKYIKLN
Sbjct: 541 EGQLRKYIKLN 551

BLAST of CSPI07G18810 vs. ExPASy TrEMBL
Match: A0A5A7UTT4 (Cytochrome P450 86B1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G00200 PE=3 SV=1)

HSP 1 Score: 1045.4 bits (2702), Expect = 8.2e-302
Identity = 521/550 (94.73%), Postives = 534/550 (97.09%), Query Frame = 0

Query: 1   MDSGNLSSSSFS---PPISIGHFGLRSLLDVQILEITFALFVFLVIHVVRQKRRHGLPIW 60
           MDSGNLSSSS S   P IS+ HFGL+SLLDVQILEI+FALFVFLVIHVVRQKRRHGLPIW
Sbjct: 1   MDSGNLSSSSSSSLPPSISVRHFGLQSLLDVQILEISFALFVFLVIHVVRQKRRHGLPIW 60

Query: 61  PIVGMLPALITATRGNLYEWITDLLCHHKGTFQFRGPWLTSLNCVVTADPRNLEHLLKTK 120
           PIVGMLPALITATRGNLYEWITDLLC HKGTFQFRGPWLTSLNCVVTADPRNLEHLLKTK
Sbjct: 61  PIVGMLPALITATRGNLYEWITDLLCRHKGTFQFRGPWLTSLNCVVTADPRNLEHLLKTK 120

Query: 121 FSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVHS 180
           FSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVHS
Sbjct: 121 FSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVHS 180

Query: 181 RLEAVLEDFEGQSTTVDLQDVLLRLTFDNVCMIAFGVDPGCLRSGLPEIPFAKAFEDATE 240
           RLEAVLEDF G+STTVDLQDVLLRLTFDNVCMIAFGVDPGCLR GLPEIPFAKAFEDATE
Sbjct: 181 RLEAVLEDFAGKSTTVDLQDVLLRLTFDNVCMIAFGVDPGCLRGGLPEIPFAKAFEDATE 240

Query: 241 VTVIRFITPTFIWKFMRYLNIGIEKKLKQSIKAVDEFAEDVIQTRKRELSTPAESQAENQ 300
           VTV+RFITPTFIWK MRYLNIGIEKKLKQSIKAVDEFAE+VIQTRKRELSTPAESQA+N+
Sbjct: 241 VTVMRFITPTFIWKAMRYLNIGIEKKLKQSIKAVDEFAENVIQTRKRELSTPAESQADNK 300

Query: 301 RSDLLTVFMKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWFFLLLDQNPDVEDKI 360
           RSDLLTVFMKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWFF LLDQNPDVEDKI
Sbjct: 301 RSDLLTVFMKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWFFWLLDQNPDVEDKI 360

Query: 361 LEEICRIVGEREEAKTRELRFDSLVFKPEEIKKMEYLHAAISEALRLYPSVPFDHKEVVE 420
           L EICRIVGEREEAKTRELRFDSLVFKPEEIKKMEYLHAAISEALRLYPSVPFDHKEVVE
Sbjct: 361 LAEICRIVGEREEAKTRELRFDSLVFKPEEIKKMEYLHAAISEALRLYPSVPFDHKEVVE 420

Query: 421 DDVFPDGTVLKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMREGRFMSESAYRFTAFN 480
           DD FPDGT+LKKG KIIYAIYAMGRMEAIWGKDCREFKPERWMR+GRFMSES YRFTAFN
Sbjct: 421 DDTFPDGTILKKGMKIIYAIYAMGRMEAIWGKDCREFKPERWMRDGRFMSESVYRFTAFN 480

Query: 481 GGPRLCLGKDFAYYQMKYVAASIISRYRVAVVRGHPVEPKLALTFYMKKGLKVNLIKREE 540
           GGPRLCLGKDFAYYQMKYVAASIISRYRVAVV GHPVEPKL+LTFYMKKGLKVNLIKREE
Sbjct: 481 GGPRLCLGKDFAYYQMKYVAASIISRYRVAVVAGHPVEPKLSLTFYMKKGLKVNLIKREE 540

Query: 541 GQLRKYIKLN 548
           GQ+RKY+KLN
Sbjct: 541 GQIRKYMKLN 550

BLAST of CSPI07G18810 vs. ExPASy TrEMBL
Match: A0A1S3CEY8 (LOW QUALITY PROTEIN: cytochrome P450 86B1-like OS=Cucumis melo OX=3656 GN=LOC103499965 PE=3 SV=1)

HSP 1 Score: 1042.0 bits (2693), Expect = 9.1e-301
Identity = 519/550 (94.36%), Postives = 532/550 (96.73%), Query Frame = 0

Query: 1   MDSGNLSSSSFS---PPISIGHFGLRSLLDVQILEITFALFVFLVIHVVRQKRRHGLPIW 60
           MDSGNLSSSS S   P IS+ HFGL+SLLDVQILEI+FALFVFLVIHVVRQKRRHGLPIW
Sbjct: 39  MDSGNLSSSSSSSLPPSISVRHFGLQSLLDVQILEISFALFVFLVIHVVRQKRRHGLPIW 98

Query: 61  PIVGMLPALITATRGNLYEWITDLLCHHKGTFQFRGPWLTSLNCVVTADPRNLEHLLKTK 120
           PIVGMLPALITATRGNLYEWITDLLC HKGTFQFRGPWLTSLNCVVTADPRNLEHLLKTK
Sbjct: 99  PIVGMLPALITATRGNLYEWITDLLCRHKGTFQFRGPWLTSLNCVVTADPRNLEHLLKTK 158

Query: 121 FSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVHS 180
           FSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVEL HS
Sbjct: 159 FSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELAHS 218

Query: 181 RLEAVLEDFEGQSTTVDLQDVLLRLTFDNVCMIAFGVDPGCLRSGLPEIPFAKAFEDATE 240
           RLEAVLEDF G+STTVDLQDVLLRLTFDNVCMIAFGVDPGCLR GLPEIPFAKAFEDATE
Sbjct: 219 RLEAVLEDFAGKSTTVDLQDVLLRLTFDNVCMIAFGVDPGCLRGGLPEIPFAKAFEDATE 278

Query: 241 VTVIRFITPTFIWKFMRYLNIGIEKKLKQSIKAVDEFAEDVIQTRKRELSTPAESQAENQ 300
           VTV+RFITPTFIWK MRYLNIGIEKKLKQSIKAVDEFAE+VIQTRKRELSTPAESQA+N+
Sbjct: 279 VTVMRFITPTFIWKAMRYLNIGIEKKLKQSIKAVDEFAENVIQTRKRELSTPAESQADNK 338

Query: 301 RSDLLTVFMKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWFFLLLDQNPDVEDKI 360
           RSDLLTVFMKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWFF LLDQNPDVEDKI
Sbjct: 339 RSDLLTVFMKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWFFWLLDQNPDVEDKI 398

Query: 361 LEEICRIVGEREEAKTRELRFDSLVFKPEEIKKMEYLHAAISEALRLYPSVPFDHKEVVE 420
           L EICRIVGEREEAKTRELRFDSLVFKPEE KKMEYLHAAISEALRLYPSVPFDHKEVVE
Sbjct: 399 LAEICRIVGEREEAKTRELRFDSLVFKPEEXKKMEYLHAAISEALRLYPSVPFDHKEVVE 458

Query: 421 DDVFPDGTVLKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMREGRFMSESAYRFTAFN 480
           DD FPDGT+LKKG KIIYAIYAMGRMEAIWGKDCREFKPERWMR+GRFMSES YRFTAFN
Sbjct: 459 DDTFPDGTILKKGMKIIYAIYAMGRMEAIWGKDCREFKPERWMRDGRFMSESVYRFTAFN 518

Query: 481 GGPRLCLGKDFAYYQMKYVAASIISRYRVAVVRGHPVEPKLALTFYMKKGLKVNLIKREE 540
           GGPRLCLGKDFAYYQMKYVAASIISRYRVAVV GHPVEPKL+LTFYMKKGLKVNLIKREE
Sbjct: 519 GGPRLCLGKDFAYYQMKYVAASIISRYRVAVVAGHPVEPKLSLTFYMKKGLKVNLIKREE 578

Query: 541 GQLRKYIKLN 548
           GQ+RKY+KLN
Sbjct: 579 GQIRKYMKLN 588

BLAST of CSPI07G18810 vs. ExPASy TrEMBL
Match: A0A6J1GQ36 (cytochrome P450 86B1-like OS=Cucurbita moschata OX=3662 GN=LOC111456469 PE=3 SV=1)

HSP 1 Score: 998.4 bits (2580), Expect = 1.1e-287
Identity = 494/548 (90.15%), Postives = 519/548 (94.71%), Query Frame = 0

Query: 1   MDSGNLSSSSFSPP-ISIGHFGLRSLLDVQILEITFALFVFLVIHVVRQKRRHGLPIWPI 60
           MDSGNLSSSS  P  +S G+FGL  LLD QILEI+FALFVFL IHVVRQ+RR+GLP+WPI
Sbjct: 15  MDSGNLSSSSALPALVSAGNFGLWPLLDFQILEISFALFVFLAIHVVRQQRRYGLPVWPI 74

Query: 61  VGMLPALITATRGNLYEWITDLLCHHKGTFQFRGPWLTSLNCVVTADPRNLEHLLKTKFS 120
           VGMLP+LITA RGNLYEWITDLL HHKGTFQFRGPW +SLNCVVTADPRNLEHLLKTKF 
Sbjct: 75  VGMLPSLITAARGNLYEWITDLLRHHKGTFQFRGPWFSSLNCVVTADPRNLEHLLKTKFP 134

Query: 121 SYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVHSRL 180
           SYPKG FFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVHSRL
Sbjct: 135 SYPKGPFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVHSRL 194

Query: 181 EAVLEDFEGQSTTVDLQDVLLRLTFDNVCMIAFGVDPGCLRSGLPEIPFAKAFEDATEVT 240
           E VLEDF G+ T VDLQDVLLRLTFDNVCMIAFGVDPGCLR GLPEIPFAKAFEDATEVT
Sbjct: 195 ETVLEDFLGRRTAVDLQDVLLRLTFDNVCMIAFGVDPGCLRGGLPEIPFAKAFEDATEVT 254

Query: 241 VIRFITPTFIWKFMRYLNIGIEKKLKQSIKAVDEFAEDVIQTRKRELSTPAESQAENQRS 300
           V+RFITPTF+WK +RYLN+G+EKKLKQSI+AVDEFAEDVIQTRKRELST A+ QAENQRS
Sbjct: 255 VLRFITPTFVWKILRYLNLGVEKKLKQSIQAVDEFAEDVIQTRKRELSTTAQPQAENQRS 314

Query: 301 DLLTVFMKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWFFLLLDQNPDVEDKILE 360
           DLLTVFM+LRDEQ RPFSDKFLRDICVNFILAGRDTSSVALSWFF LLD+NPDVEDKIL 
Sbjct: 315 DLLTVFMRLRDEQRRPFSDKFLRDICVNFILAGRDTSSVALSWFFWLLDRNPDVEDKILA 374

Query: 361 EICRIVGEREEAKTRELRFDSLVFKPEEIKKMEYLHAAISEALRLYPSVPFDHKEVVEDD 420
           EI RIVGEREEAKTRE RFDSLVFKPEEIKKMEYLHAAISEALRLYPSVP DHKEVVEDD
Sbjct: 375 EIYRIVGEREEAKTREFRFDSLVFKPEEIKKMEYLHAAISEALRLYPSVPVDHKEVVEDD 434

Query: 421 VFPDGTVLKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMREGRFMSESAYRFTAFNGG 480
           VFPDGT+LKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWM++GRFMSESAYRFTAFNGG
Sbjct: 435 VFPDGTILKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMKDGRFMSESAYRFTAFNGG 494

Query: 481 PRLCLGKDFAYYQMKYVAASIISRYRVAVVRGHPVEPKLALTFYMKKGLKVNLIKREEGQ 540
           PRLCLG+DFAYYQMKYVAASIISRYRVAVV+GHPVEPKLALTFYMK GLKVNLIKREEGQ
Sbjct: 495 PRLCLGRDFAYYQMKYVAASIISRYRVAVVKGHPVEPKLALTFYMKDGLKVNLIKREEGQ 554

Query: 541 LRKYIKLN 548
           +RKY+KLN
Sbjct: 555 IRKYVKLN 562

BLAST of CSPI07G18810 vs. ExPASy TrEMBL
Match: A0A6J1JMG1 (cytochrome P450 86B1-like OS=Cucurbita maxima OX=3661 GN=LOC111488206 PE=3 SV=1)

HSP 1 Score: 981.1 bits (2535), Expect = 1.9e-282
Identity = 487/548 (88.87%), Postives = 513/548 (93.61%), Query Frame = 0

Query: 1   MDSGNLSSSSFSPP-ISIGHFGLRSLLDVQILEITFALFVFLVIHVVRQKRRHGLPIWPI 60
           MDSGNLSSSS  P  +S  +F L  LLDVQILE++FALFVFL IHV+RQ+RR+GL +WPI
Sbjct: 15  MDSGNLSSSSALPAVVSAENFRLWPLLDVQILELSFALFVFLAIHVIRQQRRYGLSVWPI 74

Query: 61  VGMLPALITATRGNLYEWITDLLCHHKGTFQFRGPWLTSLNCVVTADPRNLEHLLKTKFS 120
           VGMLP+LITA RGNLYEWITDLL  HKGTFQFRGPW +SLNCVVTADPRNLEHLLKTKF 
Sbjct: 75  VGMLPSLITAARGNLYEWITDLLRRHKGTFQFRGPWFSSLNCVVTADPRNLEHLLKTKFP 134

Query: 121 SYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVHSRL 180
            YPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVHSRL
Sbjct: 135 YYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVHSRL 194

Query: 181 EAVLEDFEGQSTTVDLQDVLLRLTFDNVCMIAFGVDPGCLRSGLPEIPFAKAFEDATEVT 240
           E VLEDF  + T VDLQDVLLRLTFDNVCMIAFGVDPGCLR GLPEIPFAKAFEDATEVT
Sbjct: 195 ETVLEDFVERRTAVDLQDVLLRLTFDNVCMIAFGVDPGCLRGGLPEIPFAKAFEDATEVT 254

Query: 241 VIRFITPTFIWKFMRYLNIGIEKKLKQSIKAVDEFAEDVIQTRKRELSTPAESQAENQRS 300
           V+RFITPTF+WK +RYLN+G+EKKLKQSIK VDEFAEDVIQTRKRELST A+ QA NQRS
Sbjct: 255 VLRFITPTFVWKILRYLNLGVEKKLKQSIKTVDEFAEDVIQTRKRELSTTAQPQANNQRS 314

Query: 301 DLLTVFMKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWFFLLLDQNPDVEDKILE 360
           DLLTVFM+LRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWFF LLDQNPDVE+KIL 
Sbjct: 315 DLLTVFMRLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWFFWLLDQNPDVENKILA 374

Query: 361 EICRIVGEREEAKTRELRFDSLVFKPEEIKKMEYLHAAISEALRLYPSVPFDHKEVVEDD 420
           EI RIVGER EAKTRE RFDSLVFKPEEIKKMEYLHAAISEALRLYPSVP DHKEVVEDD
Sbjct: 375 EIYRIVGERGEAKTREFRFDSLVFKPEEIKKMEYLHAAISEALRLYPSVPVDHKEVVEDD 434

Query: 421 VFPDGTVLKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMREGRFMSESAYRFTAFNGG 480
           VFPDGT+LKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWM++GRFMSESAYRFTAFNGG
Sbjct: 435 VFPDGTILKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMKDGRFMSESAYRFTAFNGG 494

Query: 481 PRLCLGKDFAYYQMKYVAASIISRYRVAVVRGHPVEPKLALTFYMKKGLKVNLIKREEGQ 540
           PRLCLG+DFAYYQMKYVAASIISRYRVAVV+GHPVEPKLALTFYMK GLKVNLIKREEGQ
Sbjct: 495 PRLCLGRDFAYYQMKYVAASIISRYRVAVVKGHPVEPKLALTFYMKDGLKVNLIKREEGQ 554

Query: 541 LRKYIKLN 548
           +RKY+KLN
Sbjct: 555 IRKYMKLN 562

BLAST of CSPI07G18810 vs. NCBI nr
Match: XP_004135954.3 (cytochrome P450 86B1 [Cucumis sativus] >KAE8646384.1 hypothetical protein Csa_015511 [Cucumis sativus])

HSP 1 Score: 1086.6 bits (2809), Expect = 0.0e+00
Identity = 544/551 (98.73%), Postives = 546/551 (99.09%), Query Frame = 0

Query: 1   MDSGNL----SSSSFSPPISIGHFGLRSLLDVQILEITFALFVFLVIHVVRQKRRHGLPI 60
           MDSGNL    SSSSFSPPISIGHFGLRSLLDVQILEITFALFVFLVIHVVRQKRRHGLPI
Sbjct: 39  MDSGNLSSSSSSSSFSPPISIGHFGLRSLLDVQILEITFALFVFLVIHVVRQKRRHGLPI 98

Query: 61  WPIVGMLPALITATRGNLYEWITDLLCHHKGTFQFRGPWLTSLNCVVTADPRNLEHLLKT 120
           WPIVGMLPALITATRGNLYEWITDLLC HKGTFQFRGPWLTSLNCVVTADPRNLEHLLKT
Sbjct: 99  WPIVGMLPALITATRGNLYEWITDLLCRHKGTFQFRGPWLTSLNCVVTADPRNLEHLLKT 158

Query: 121 KFSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVH 180
           KFSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVH
Sbjct: 159 KFSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVH 218

Query: 181 SRLEAVLEDFEGQSTTVDLQDVLLRLTFDNVCMIAFGVDPGCLRSGLPEIPFAKAFEDAT 240
           SRLEAVLEDFEGQ+TTVDLQDVLLRLTFDNVCMIAFGVDPGCLRSGLPEIPFAKAFEDAT
Sbjct: 219 SRLEAVLEDFEGQATTVDLQDVLLRLTFDNVCMIAFGVDPGCLRSGLPEIPFAKAFEDAT 278

Query: 241 EVTVIRFITPTFIWKFMRYLNIGIEKKLKQSIKAVDEFAEDVIQTRKRELSTPAESQAEN 300
           EVTVIRFITPTFIWKFMRYLNIGIEKKLKQSIKAVDEFAEDVIQTRKRELSTPAESQAEN
Sbjct: 279 EVTVIRFITPTFIWKFMRYLNIGIEKKLKQSIKAVDEFAEDVIQTRKRELSTPAESQAEN 338

Query: 301 QRSDLLTVFMKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWFFLLLDQNPDVEDK 360
           QRSDLLTVFMKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWFFLLLDQNPDVEDK
Sbjct: 339 QRSDLLTVFMKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWFFLLLDQNPDVEDK 398

Query: 361 ILEEICRIVGEREEAKTRELRFDSLVFKPEEIKKMEYLHAAISEALRLYPSVPFDHKEVV 420
           ILEEICRIVGEREEAKTRELRFDSLVFKPEEIK+MEYLHAAISEALRLYPSVPFDHKEVV
Sbjct: 399 ILEEICRIVGEREEAKTRELRFDSLVFKPEEIKRMEYLHAAISEALRLYPSVPFDHKEVV 458

Query: 421 EDDVFPDGTVLKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMREGRFMSESAYRFTAF 480
           EDDVFPDGTVLKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMREGRFMSESAYRFTAF
Sbjct: 459 EDDVFPDGTVLKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMREGRFMSESAYRFTAF 518

Query: 481 NGGPRLCLGKDFAYYQMKYVAASIISRYRVAVVRGHPVEPKLALTFYMKKGLKVNLIKRE 540
           NGGPRLCLGKDFAYYQMKYVAASIISRYRVAVVRGHPVEPKLALTFYMKKGLKVNLIKRE
Sbjct: 519 NGGPRLCLGKDFAYYQMKYVAASIISRYRVAVVRGHPVEPKLALTFYMKKGLKVNLIKRE 578

Query: 541 EGQLRKYIKLN 548
           EGQLRKYIKLN
Sbjct: 579 EGQLRKYIKLN 589

BLAST of CSPI07G18810 vs. NCBI nr
Match: KAA0058578.1 (cytochrome P450 86B1-like [Cucumis melo var. makuwa] >TYK10378.1 cytochrome P450 86B1-like [Cucumis melo var. makuwa])

HSP 1 Score: 1045.4 bits (2702), Expect = 1.7e-301
Identity = 521/550 (94.73%), Postives = 534/550 (97.09%), Query Frame = 0

Query: 1   MDSGNLSSSSFS---PPISIGHFGLRSLLDVQILEITFALFVFLVIHVVRQKRRHGLPIW 60
           MDSGNLSSSS S   P IS+ HFGL+SLLDVQILEI+FALFVFLVIHVVRQKRRHGLPIW
Sbjct: 1   MDSGNLSSSSSSSLPPSISVRHFGLQSLLDVQILEISFALFVFLVIHVVRQKRRHGLPIW 60

Query: 61  PIVGMLPALITATRGNLYEWITDLLCHHKGTFQFRGPWLTSLNCVVTADPRNLEHLLKTK 120
           PIVGMLPALITATRGNLYEWITDLLC HKGTFQFRGPWLTSLNCVVTADPRNLEHLLKTK
Sbjct: 61  PIVGMLPALITATRGNLYEWITDLLCRHKGTFQFRGPWLTSLNCVVTADPRNLEHLLKTK 120

Query: 121 FSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVHS 180
           FSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVHS
Sbjct: 121 FSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVHS 180

Query: 181 RLEAVLEDFEGQSTTVDLQDVLLRLTFDNVCMIAFGVDPGCLRSGLPEIPFAKAFEDATE 240
           RLEAVLEDF G+STTVDLQDVLLRLTFDNVCMIAFGVDPGCLR GLPEIPFAKAFEDATE
Sbjct: 181 RLEAVLEDFAGKSTTVDLQDVLLRLTFDNVCMIAFGVDPGCLRGGLPEIPFAKAFEDATE 240

Query: 241 VTVIRFITPTFIWKFMRYLNIGIEKKLKQSIKAVDEFAEDVIQTRKRELSTPAESQAENQ 300
           VTV+RFITPTFIWK MRYLNIGIEKKLKQSIKAVDEFAE+VIQTRKRELSTPAESQA+N+
Sbjct: 241 VTVMRFITPTFIWKAMRYLNIGIEKKLKQSIKAVDEFAENVIQTRKRELSTPAESQADNK 300

Query: 301 RSDLLTVFMKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWFFLLLDQNPDVEDKI 360
           RSDLLTVFMKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWFF LLDQNPDVEDKI
Sbjct: 301 RSDLLTVFMKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWFFWLLDQNPDVEDKI 360

Query: 361 LEEICRIVGEREEAKTRELRFDSLVFKPEEIKKMEYLHAAISEALRLYPSVPFDHKEVVE 420
           L EICRIVGEREEAKTRELRFDSLVFKPEEIKKMEYLHAAISEALRLYPSVPFDHKEVVE
Sbjct: 361 LAEICRIVGEREEAKTRELRFDSLVFKPEEIKKMEYLHAAISEALRLYPSVPFDHKEVVE 420

Query: 421 DDVFPDGTVLKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMREGRFMSESAYRFTAFN 480
           DD FPDGT+LKKG KIIYAIYAMGRMEAIWGKDCREFKPERWMR+GRFMSES YRFTAFN
Sbjct: 421 DDTFPDGTILKKGMKIIYAIYAMGRMEAIWGKDCREFKPERWMRDGRFMSESVYRFTAFN 480

Query: 481 GGPRLCLGKDFAYYQMKYVAASIISRYRVAVVRGHPVEPKLALTFYMKKGLKVNLIKREE 540
           GGPRLCLGKDFAYYQMKYVAASIISRYRVAVV GHPVEPKL+LTFYMKKGLKVNLIKREE
Sbjct: 481 GGPRLCLGKDFAYYQMKYVAASIISRYRVAVVAGHPVEPKLSLTFYMKKGLKVNLIKREE 540

Query: 541 GQLRKYIKLN 548
           GQ+RKY+KLN
Sbjct: 541 GQIRKYMKLN 550

BLAST of CSPI07G18810 vs. NCBI nr
Match: XP_008461361.1 (PREDICTED: LOW QUALITY PROTEIN: cytochrome P450 86B1-like [Cucumis melo])

HSP 1 Score: 1042.0 bits (2693), Expect = 1.9e-300
Identity = 519/550 (94.36%), Postives = 532/550 (96.73%), Query Frame = 0

Query: 1   MDSGNLSSSSFS---PPISIGHFGLRSLLDVQILEITFALFVFLVIHVVRQKRRHGLPIW 60
           MDSGNLSSSS S   P IS+ HFGL+SLLDVQILEI+FALFVFLVIHVVRQKRRHGLPIW
Sbjct: 39  MDSGNLSSSSSSSLPPSISVRHFGLQSLLDVQILEISFALFVFLVIHVVRQKRRHGLPIW 98

Query: 61  PIVGMLPALITATRGNLYEWITDLLCHHKGTFQFRGPWLTSLNCVVTADPRNLEHLLKTK 120
           PIVGMLPALITATRGNLYEWITDLLC HKGTFQFRGPWLTSLNCVVTADPRNLEHLLKTK
Sbjct: 99  PIVGMLPALITATRGNLYEWITDLLCRHKGTFQFRGPWLTSLNCVVTADPRNLEHLLKTK 158

Query: 121 FSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVHS 180
           FSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVEL HS
Sbjct: 159 FSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELAHS 218

Query: 181 RLEAVLEDFEGQSTTVDLQDVLLRLTFDNVCMIAFGVDPGCLRSGLPEIPFAKAFEDATE 240
           RLEAVLEDF G+STTVDLQDVLLRLTFDNVCMIAFGVDPGCLR GLPEIPFAKAFEDATE
Sbjct: 219 RLEAVLEDFAGKSTTVDLQDVLLRLTFDNVCMIAFGVDPGCLRGGLPEIPFAKAFEDATE 278

Query: 241 VTVIRFITPTFIWKFMRYLNIGIEKKLKQSIKAVDEFAEDVIQTRKRELSTPAESQAENQ 300
           VTV+RFITPTFIWK MRYLNIGIEKKLKQSIKAVDEFAE+VIQTRKRELSTPAESQA+N+
Sbjct: 279 VTVMRFITPTFIWKAMRYLNIGIEKKLKQSIKAVDEFAENVIQTRKRELSTPAESQADNK 338

Query: 301 RSDLLTVFMKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWFFLLLDQNPDVEDKI 360
           RSDLLTVFMKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWFF LLDQNPDVEDKI
Sbjct: 339 RSDLLTVFMKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWFFWLLDQNPDVEDKI 398

Query: 361 LEEICRIVGEREEAKTRELRFDSLVFKPEEIKKMEYLHAAISEALRLYPSVPFDHKEVVE 420
           L EICRIVGEREEAKTRELRFDSLVFKPEE KKMEYLHAAISEALRLYPSVPFDHKEVVE
Sbjct: 399 LAEICRIVGEREEAKTRELRFDSLVFKPEEXKKMEYLHAAISEALRLYPSVPFDHKEVVE 458

Query: 421 DDVFPDGTVLKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMREGRFMSESAYRFTAFN 480
           DD FPDGT+LKKG KIIYAIYAMGRMEAIWGKDCREFKPERWMR+GRFMSES YRFTAFN
Sbjct: 459 DDTFPDGTILKKGMKIIYAIYAMGRMEAIWGKDCREFKPERWMRDGRFMSESVYRFTAFN 518

Query: 481 GGPRLCLGKDFAYYQMKYVAASIISRYRVAVVRGHPVEPKLALTFYMKKGLKVNLIKREE 540
           GGPRLCLGKDFAYYQMKYVAASIISRYRVAVV GHPVEPKL+LTFYMKKGLKVNLIKREE
Sbjct: 519 GGPRLCLGKDFAYYQMKYVAASIISRYRVAVVAGHPVEPKLSLTFYMKKGLKVNLIKREE 578

Query: 541 GQLRKYIKLN 548
           GQ+RKY+KLN
Sbjct: 579 GQIRKYMKLN 588

BLAST of CSPI07G18810 vs. NCBI nr
Match: XP_022954102.1 (cytochrome P450 86B1-like [Cucurbita moschata])

HSP 1 Score: 998.4 bits (2580), Expect = 2.4e-287
Identity = 494/548 (90.15%), Postives = 519/548 (94.71%), Query Frame = 0

Query: 1   MDSGNLSSSSFSPP-ISIGHFGLRSLLDVQILEITFALFVFLVIHVVRQKRRHGLPIWPI 60
           MDSGNLSSSS  P  +S G+FGL  LLD QILEI+FALFVFL IHVVRQ+RR+GLP+WPI
Sbjct: 15  MDSGNLSSSSALPALVSAGNFGLWPLLDFQILEISFALFVFLAIHVVRQQRRYGLPVWPI 74

Query: 61  VGMLPALITATRGNLYEWITDLLCHHKGTFQFRGPWLTSLNCVVTADPRNLEHLLKTKFS 120
           VGMLP+LITA RGNLYEWITDLL HHKGTFQFRGPW +SLNCVVTADPRNLEHLLKTKF 
Sbjct: 75  VGMLPSLITAARGNLYEWITDLLRHHKGTFQFRGPWFSSLNCVVTADPRNLEHLLKTKFP 134

Query: 121 SYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVHSRL 180
           SYPKG FFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVHSRL
Sbjct: 135 SYPKGPFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVHSRL 194

Query: 181 EAVLEDFEGQSTTVDLQDVLLRLTFDNVCMIAFGVDPGCLRSGLPEIPFAKAFEDATEVT 240
           E VLEDF G+ T VDLQDVLLRLTFDNVCMIAFGVDPGCLR GLPEIPFAKAFEDATEVT
Sbjct: 195 ETVLEDFLGRRTAVDLQDVLLRLTFDNVCMIAFGVDPGCLRGGLPEIPFAKAFEDATEVT 254

Query: 241 VIRFITPTFIWKFMRYLNIGIEKKLKQSIKAVDEFAEDVIQTRKRELSTPAESQAENQRS 300
           V+RFITPTF+WK +RYLN+G+EKKLKQSI+AVDEFAEDVIQTRKRELST A+ QAENQRS
Sbjct: 255 VLRFITPTFVWKILRYLNLGVEKKLKQSIQAVDEFAEDVIQTRKRELSTTAQPQAENQRS 314

Query: 301 DLLTVFMKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWFFLLLDQNPDVEDKILE 360
           DLLTVFM+LRDEQ RPFSDKFLRDICVNFILAGRDTSSVALSWFF LLD+NPDVEDKIL 
Sbjct: 315 DLLTVFMRLRDEQRRPFSDKFLRDICVNFILAGRDTSSVALSWFFWLLDRNPDVEDKILA 374

Query: 361 EICRIVGEREEAKTRELRFDSLVFKPEEIKKMEYLHAAISEALRLYPSVPFDHKEVVEDD 420
           EI RIVGEREEAKTRE RFDSLVFKPEEIKKMEYLHAAISEALRLYPSVP DHKEVVEDD
Sbjct: 375 EIYRIVGEREEAKTREFRFDSLVFKPEEIKKMEYLHAAISEALRLYPSVPVDHKEVVEDD 434

Query: 421 VFPDGTVLKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMREGRFMSESAYRFTAFNGG 480
           VFPDGT+LKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWM++GRFMSESAYRFTAFNGG
Sbjct: 435 VFPDGTILKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMKDGRFMSESAYRFTAFNGG 494

Query: 481 PRLCLGKDFAYYQMKYVAASIISRYRVAVVRGHPVEPKLALTFYMKKGLKVNLIKREEGQ 540
           PRLCLG+DFAYYQMKYVAASIISRYRVAVV+GHPVEPKLALTFYMK GLKVNLIKREEGQ
Sbjct: 495 PRLCLGRDFAYYQMKYVAASIISRYRVAVVKGHPVEPKLALTFYMKDGLKVNLIKREEGQ 554

Query: 541 LRKYIKLN 548
           +RKY+KLN
Sbjct: 555 IRKYVKLN 562

BLAST of CSPI07G18810 vs. NCBI nr
Match: KAG7014270.1 (Cytochrome P450 86B1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 996.9 bits (2576), Expect = 6.9e-287
Identity = 492/548 (89.78%), Postives = 519/548 (94.71%), Query Frame = 0

Query: 1   MDSGNLSSSSFSPP-ISIGHFGLRSLLDVQILEITFALFVFLVIHVVRQKRRHGLPIWPI 60
           MDSGNLS+SS  P  +S G+FGL  LLD QILEI+FALF+FL IHVVRQ+RR+GLP+WPI
Sbjct: 15  MDSGNLSTSSAPPALVSAGNFGLWPLLDFQILEISFALFMFLAIHVVRQQRRYGLPVWPI 74

Query: 61  VGMLPALITATRGNLYEWITDLLCHHKGTFQFRGPWLTSLNCVVTADPRNLEHLLKTKFS 120
           VGMLP+LITA RGNLYEWITDLL  HKGTFQFRGPW +SLNCVVTADPRNLEHLLKTKF 
Sbjct: 75  VGMLPSLITAARGNLYEWITDLLRRHKGTFQFRGPWFSSLNCVVTADPRNLEHLLKTKFP 134

Query: 121 SYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVHSRL 180
           SYPKG FFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVHSRL
Sbjct: 135 SYPKGPFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVHSRL 194

Query: 181 EAVLEDFEGQSTTVDLQDVLLRLTFDNVCMIAFGVDPGCLRSGLPEIPFAKAFEDATEVT 240
           E VLEDF G+ T VDLQDVLLRLTFDNVCMIAFGVDPGCLR GLPEIPFAKAFEDATEVT
Sbjct: 195 ETVLEDFVGRRTAVDLQDVLLRLTFDNVCMIAFGVDPGCLRGGLPEIPFAKAFEDATEVT 254

Query: 241 VIRFITPTFIWKFMRYLNIGIEKKLKQSIKAVDEFAEDVIQTRKRELSTPAESQAENQRS 300
           V+RFITPTF+WK +RYLN+G+EKKLKQSI+AVDEFAEDVIQTRKRELST A+ QAENQRS
Sbjct: 255 VLRFITPTFVWKILRYLNLGVEKKLKQSIQAVDEFAEDVIQTRKRELSTTAQPQAENQRS 314

Query: 301 DLLTVFMKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWFFLLLDQNPDVEDKILE 360
           DLLTVFM+LRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWFF LLD+NPDVEDKIL 
Sbjct: 315 DLLTVFMRLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWFFWLLDRNPDVEDKILA 374

Query: 361 EICRIVGEREEAKTRELRFDSLVFKPEEIKKMEYLHAAISEALRLYPSVPFDHKEVVEDD 420
           EI RIVGEREEAKTRE RFDSLVFKPEEIKKMEYLHAAISEALRLYPSVP DHKEVVEDD
Sbjct: 375 EIYRIVGEREEAKTREFRFDSLVFKPEEIKKMEYLHAAISEALRLYPSVPVDHKEVVEDD 434

Query: 421 VFPDGTVLKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMREGRFMSESAYRFTAFNGG 480
           VFPDGT+LKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWM++GRFMSESAYRFTAFNGG
Sbjct: 435 VFPDGTILKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMKDGRFMSESAYRFTAFNGG 494

Query: 481 PRLCLGKDFAYYQMKYVAASIISRYRVAVVRGHPVEPKLALTFYMKKGLKVNLIKREEGQ 540
           PRLCLG+DFAYYQMKYVAASIISRYRVAVV+GHPVEPKLALTFYMK GLKVNLIKREEGQ
Sbjct: 495 PRLCLGRDFAYYQMKYVAASIISRYRVAVVKGHPVEPKLALTFYMKDGLKVNLIKREEGQ 554

Query: 541 LRKYIKLN 548
           +RKY+KLN
Sbjct: 555 IRKYVKLN 562

BLAST of CSPI07G18810 vs. TAIR 10
Match: AT5G23190.1 (cytochrome P450, family 86, subfamily B, polypeptide 1 )

HSP 1 Score: 767.3 bits (1980), Expect = 8.3e-222
Identity = 376/537 (70.02%), Postives = 439/537 (81.75%), Query Frame = 0

Query: 7   SSSSFSPPISIGHFGLRSLLDVQILEITFALFVFLVIHVVRQKRRHGLPIWPIVGMLPAL 66
           SSSS  P +S   F LR   DVQILE+  A+FVF+ IH +RQK+  GLP+WP +GMLP+L
Sbjct: 18  SSSSSDPLVSRRLFLLR---DVQILELLIAIFVFVAIHALRQKKYQGLPVWPFLGMLPSL 77

Query: 67  ITATRGNLYEWITDLLCHHKGTFQFRGPWLTSLNCVVTADPRNLEHLLKTKFSSYPKGSF 126
               RGN+YEW++D+LC   GTFQFRGPW +SLN  +T DPRN+EHLLK +FS +PKGS+
Sbjct: 78  AFGLRGNIYEWLSDVLCLQNGTFQFRGPWFSSLNSTITCDPRNVEHLLKNRFSVFPKGSY 137

Query: 127 FRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVHSRLEAVLEDF 186
           FRD +RDLLGDGIF ADDETWQRQRK ASIEFHSA+FRQ+T +SL ELVH RL  VLE  
Sbjct: 138 FRDNLRDLLGDGIFNADDETWQRQRKTASIEFHSAKFRQLTTQSLFELVHKRLLPVLETS 197

Query: 187 EGQSTTVDLQDVLLRLTFDNVCMIAFGVDPGCLRSGLPEIPFAKAFEDATEVTVIRFITP 246
              S+ +DLQDVLLRLTFDNVCMIAFGVDPGCL    P IPFAKAFEDATE  V+RF+ P
Sbjct: 198 VKSSSPIDLQDVLLRLTFDNVCMIAFGVDPGCLGPDQPVIPFAKAFEDATEAAVVRFVMP 257

Query: 247 TFIWKFMRYLNIGIEKKLKQSIKAVDEFAEDVIQTRKRELSTPAESQAENQRSDLLTVFM 306
           T +WKFMRYL+IG EKKLK+SIK VD+FA++VI+TRK+ELS   E+    +RSDLLTVFM
Sbjct: 258 TCVWKFMRYLDIGTEKKLKESIKGVDDFADEVIRTRKKELSLEGET---TKRSDLLTVFM 317

Query: 307 KLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWFFLLLDQNPDVEDKILEEICRIVG 366
            LRDE+G  FSDKFLRDICVNFILAGRDTSSVALSWFF LL++NP+VE+KI+ E+C+I+ 
Sbjct: 318 GLRDEKGESFSDKFLRDICVNFILAGRDTSSVALSWFFWLLEKNPEVEEKIMVEMCKILR 377

Query: 367 EREEAKTRELRFDSLVFKPEEIKKMEYLHAAISEALRLYPSVPFDHKEVVEDDVFPDGTV 426
           +R++    E      VF PEEIKKM+YL AA+SEALRLYPSVP DHKEV EDDVFPDGT+
Sbjct: 378 QRDDHGNAEKSDYEPVFGPEEIKKMDYLQAALSEALRLYPSVPVDHKEVQEDDVFPDGTM 437

Query: 427 LKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMREGRFMSESAYRFTAFNGGPRLCLGK 486
           LKKG K+IYAIYAMGRMEAIWGKDC EF+PERW+R+GRFMSESAY+FTAFNGGPRLCLGK
Sbjct: 438 LKKGDKVIYAIYAMGRMEAIWGKDCLEFRPERWLRDGRFMSESAYKFTAFNGGPRLCLGK 497

Query: 487 DFAYYQMKYVAASIISRYRVAVVRGHPVEPKLALTFYMKKGLKVNLIKREEGQLRKY 544
           DFAYYQMK  AA+I+ RY+V VV GH VEPKLALT YMK GL VNLI R   ++ +Y
Sbjct: 498 DFAYYQMKSTAAAIVYRYKVKVVNGHKVEPKLALTMYMKHGLMVNLINRSVSEIDQY 548

BLAST of CSPI07G18810 vs. TAIR 10
Match: AT5G08250.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 716.5 bits (1848), Expect = 1.7e-206
Identity = 345/493 (69.98%), Postives = 412/493 (83.57%), Query Frame = 0

Query: 54  LPIWPIVGMLPALITATRGNLYEWITDLLCHHKGTFQFRGPWLTSLNCVVTADPRNLEHL 113
           +P+WP+VGMLP+LI+A R N+YEW++D+L    GTF+FRGPW ++LNCVVT DPRN+EHL
Sbjct: 1   MPVWPLVGMLPSLISAVRSNIYEWLSDVLISQNGTFRFRGPWFSTLNCVVTCDPRNVEHL 60

Query: 114 LKTKFSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVE 173
           LKT+FS YPKGS+FR+T++DLLGDGIF  DD TWQRQRK AS+EFHSA+FRQ+T +SL E
Sbjct: 61  LKTRFSIYPKGSYFRETMQDLLGDGIFNTDDGTWQRQRKAASVEFHSAKFRQLTSQSLHE 120

Query: 174 LVHSRLEAVLEDFEGQSTTVDLQDVLLRLTFDNVCMIAFGVDPGCLRSGLPEIPFAKAFE 233
           LVH+RL  VLE     S  +DLQD+LLRLTFDNVCMIAFGVDPGCL   LPEIPFAKAFE
Sbjct: 121 LVHNRLLPVLE----TSGKIDLQDILLRLTFDNVCMIAFGVDPGCLSPKLPEIPFAKAFE 180

Query: 234 DATEVTVIRFITPTFIWKFMRYLNIGIEKKLKQSIKAVDEFAEDVIQTRKRELSTPAESQ 293
           DATE TV+RF+ P F+WK MR LN+G EKKLK+SI  VD+FAE+VI+TRK+E+S   E  
Sbjct: 181 DATEATVVRFVMPKFVWKLMRSLNLGTEKKLKESINGVDDFAEEVIRTRKKEMSLETEIA 240

Query: 294 AENQRSDLLTVFMKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWFFLLLDQNPDV 353
              +R DLLT+FM LRDE G+ FSDKFLRDICVNFILAGRDTSSVALSWFF L+++NP+V
Sbjct: 241 ---KRPDLLTIFMGLRDENGQKFSDKFLRDICVNFILAGRDTSSVALSWFFWLIEKNPEV 300

Query: 354 EDKILEEICRIVGER-EEAKTRELRFDSLVFKPEEIKKMEYLHAAISEALRLYPSVPFDH 413
           E+KI+  IC+I+ +R +   T++      VF+PEEIKKM+YL AA+SE LRLYPSVP DH
Sbjct: 301 EEKIMMGICKILEQRVDHGDTKKNMEYEPVFRPEEIKKMDYLQAALSETLRLYPSVPVDH 360

Query: 414 KEVVEDDVFPDGTVLKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMREGRFMSESAYR 473
           KEV+EDDVFPDGT LKKG K+IYAIYAMGRME IWGKDCREFKPERW+R+GR+MSESAY+
Sbjct: 361 KEVLEDDVFPDGTKLKKGEKVIYAIYAMGRMETIWGKDCREFKPERWLRDGRYMSESAYK 420

Query: 474 FTAFNGGPRLCLGKDFAYYQMKYVAASIISRYRVAV--VRGHPVEPKLALTFYMKKGLKV 533
           FTAFNGGPRLCLGKDFAYYQM+YVAA+II RY+V V    GH VEPK+ALT YMK GLKV
Sbjct: 421 FTAFNGGPRLCLGKDFAYYQMRYVAAAIIYRYKVRVDDKGGHKVEPKMALTMYMKHGLKV 480

Query: 534 NLIKREEGQLRKY 544
           N++KR   ++  Y
Sbjct: 481 NMVKRSVSEIDHY 486

BLAST of CSPI07G18810 vs. TAIR 10
Match: AT1G24540.1 (cytochrome P450, family 86, subfamily C, polypeptide 1 )

HSP 1 Score: 491.1 bits (1263), Expect = 1.1e-138
Identity = 241/517 (46.62%), Postives = 347/517 (67.12%), Query Frame = 0

Query: 28  VQILEITFALFVFLVIHVVRQK--RRHGLPIWPIVGMLPALITATRGNLYEWITDLLCHH 87
           +++ ++  AL    ++  +R+K   + G  +WP++G++P ++   + +L+ W T  +   
Sbjct: 15  LRLSDVALALVGLFLLSYLREKLVSKGGPVMWPVLGIIP-MLALNKHDLFTWCTRCVVRS 74

Query: 88  KGTFQFRGPWLTSLNCVVTADPRNLEHLLKTKFSSYPKGSFFRDTVRDLLGDGIFGADDE 147
            GTF +RG W      ++TADP N+EH+LKT F +YPKG+F+R+  RDLL DGIF ADDE
Sbjct: 75  GGTFHYRGIWFGGAYGIMTADPANVEHILKTNFKNYPKGAFYRERFRDLLEDGIFNADDE 134

Query: 148 TWQRQRKVASIEFHSARFRQMTVESLVELVHSRLEAVLEDFEGQSTTVDLQDVLLRLTFD 207
            W+ +R+VA  E HS+RF + T  ++ +LV  +L  ++E+        DLQD+LLR TFD
Sbjct: 135 LWKEERRVAKTEMHSSRFLEHTFTTMRDLVDQKLVPLMENLSTSKRVFDLQDLLLRFTFD 194

Query: 208 NVCMIAFGVDPGCLRSGLPEIPFAKAFEDATEVTVIRFITPTFIWKFMRYLNIGIEKKLK 267
           N+C+ AFGV PG L +GLPEIPFAKAFEDATE T+ RF+ P F+WK MR+L IG E+KL 
Sbjct: 195 NICISAFGVYPGSLETGLPEIPFAKAFEDATEYTLARFLIPPFVWKPMRFLGIGYERKLN 254

Query: 268 QSIKAVDEFAEDVIQTRKRELSTPAESQAENQRSDLLTVFMKLRDE------QGRPFSDK 327
            +++ V  FA   ++ R+ ++         N  +DLL+  M+   E      +G  FSDK
Sbjct: 255 NAVRIVHAFANKTVRERRNKMRKLGNL---NDYADLLSRLMQREYEKEEDTTRGNYFSDK 314

Query: 328 FLRDICVNFILAGRDTSSVALSWFFLLLDQNPDVEDKILEEICRIVGEREEAKTRELRFD 387
           + R+ C +FI+AGRDT+SVAL WFF L+ ++P+VE +IL EI  I  +    +T +    
Sbjct: 315 YFREFCTSFIIAGRDTTSVALVWFFWLVQKHPEVEKRILREIREIKRKLTTQETED---- 374

Query: 388 SLVFKPEEIKKMEYLHAAISEALRLYPSVPFDHKEVVEDDVFPDGTVLKKGTKIIYAIYA 447
              F+ E+ ++M YL AA++E+LRLYPSVP + K+ +EDDV PDGT +KKG +I Y++Y+
Sbjct: 375 --QFEAEDFREMVYLQAALTESLRLYPSVPMEMKQALEDDVLPDGTRVKKGARIHYSVYS 434

Query: 448 MGRMEAIWGKDCREFKPERWMREGRFMSESAYRFTAFNGGPRLCLGKDFAYYQMKYVAAS 507
           MGR+E+IWGKD  EFKPERW++EGR +SE  +++  FNGGPRLC+GK FAY QMK VAA+
Sbjct: 435 MGRIESIWGKDWEEFKPERWIKEGRIVSEDQFKYVVFNGGPRLCVGKKFAYTQMKMVAAA 494

Query: 508 IISRYRVAVVRGHPVEPKLALTFYMKKGLKVNLIKRE 537
           I+ RY V VV+G  + PKL  T YMK G+ V L  R+
Sbjct: 495 ILMRYSVKVVQGQEIVPKLTTTLYMKNGMNVMLQPRD 521

BLAST of CSPI07G18810 vs. TAIR 10
Match: AT1G13140.1 (cytochrome P450, family 86, subfamily C, polypeptide 3 )

HSP 1 Score: 464.9 bits (1195), Expect = 8.8e-131
Identity = 227/507 (44.77%), Postives = 333/507 (65.68%), Query Frame = 0

Query: 32  EITFALFVFLVIHVVRQK--RRHGLPIWPIVGMLPALITATRGNLYEWITDLLCHHKGTF 91
           +++ AL    V   VR+K  ++ G  IWP+ G+ P      R ++Y W T  L   +GTF
Sbjct: 22  DVSLALLGLFVFCCVREKVTKKLGPTIWPVFGITPEFF-FHRNDVYGWATRCLKKCRGTF 81

Query: 92  QFRGPWLTSLNCVVTADPRNLEHLLKTKFSSYPKGSFFRDTVRDLLGDGIFGADDETWQR 151
            + G WL      VT  P N+E++LKT F ++PKG+FF++   DLL DGIF AD E+W+ 
Sbjct: 82  LYNGIWLGGSYGAVTCVPANVEYMLKTNFKNFPKGAFFKERFNDLLEDGIFNADAESWKE 141

Query: 152 QRKVASIEFHSARFRQMTVESLVELVHSRLEAVLEDFEGQSTTVDLQDVLLRLTFDNVCM 211
           QR++   E HS RF + + ++  +LV  +L  V+E F       DLQDVLLRLTFDN+C+
Sbjct: 142 QRRIIITEMHSTRFVEHSFQTTQDLVRKKLLKVMESFARSQEAFDLQDVLLRLTFDNICI 201

Query: 212 IAFGVDPGCLRSGLPEIPFAKAFEDATEVTVIRFITPTFIWKFMRYLNIGIEKKLKQSIK 271
              G DPG L S LP +PFA+AFE+ATE T+ RF+ P FIWK +++ +IG EK L++++ 
Sbjct: 202 AGLGDDPGTLDSDLPLVPFAQAFEEATESTMFRFMIPPFIWKPLKFFDIGYEKGLRKAVD 261

Query: 272 AVDEFAEDVIQTRKRELSTPAESQAENQRSDLLTVFMKLRD----EQGRPFSDKFLRDIC 331
            V EF + ++  R  +L    E      RSD+L+  +++      ++  P + KF R  C
Sbjct: 262 VVHEFVDKMVVDRICKLK---EEGTLGNRSDVLSRIIEIESHKTTDEKDPSTIKFFRQFC 321

Query: 332 VNFILAGRDTSSVALSWFFLLLDQNPDVEDKILEEICRIVGEREEAKTRELRFDSLVFKP 391
            +FILAGRDTSSVAL+WFF ++ ++P+VE+KI+ EI  I+ +R ++ T +   +  +F  
Sbjct: 322 TSFILAGRDTSSVALTWFFWVIQKHPEVENKIIREISEILRQRGDSPTSK---NESLFTV 381

Query: 392 EEIKKMEYLHAAISEALRLYPSVPFDHKEVVEDDVFPDGTVLKKGTKIIYAIYAMGRMEA 451
           +E+  M YL AA+SE +RLYP +P + K+ +EDDVFPDGT ++KG+++ +A YAMGRME+
Sbjct: 382 KELNDMVYLQAALSETMRLYPPIPMEMKQAIEDDVFPDGTFIRKGSRVYFATYAMGRMES 441

Query: 452 IWGKDCREFKPERWMREGRFMSESAYRFTAFNGGPRLCLGKDFAYYQMKYVAASIISRYR 511
           IWGKDC  FKPERW++ G F+++  +++  FN GPRLCLGK FAY QMK +AAS++SRY 
Sbjct: 442 IWGKDCESFKPERWIQSGNFVNDDQFKYVVFNAGPRLCLGKTFAYLQMKTIAASVLSRYS 501

Query: 512 VAVVRGHPVEPKLALTFYMKKGLKVNL 533
           + V + H V P++  T YM+ GLKV +
Sbjct: 502 IKVAKDHVVVPRVTTTLYMRHGLKVTI 521

BLAST of CSPI07G18810 vs. TAIR 10
Match: AT1G63710.1 (cytochrome P450, family 86, subfamily A, polypeptide 7 )

HSP 1 Score: 458.0 bits (1177), Expect = 1.1e-128
Identity = 243/517 (47.00%), Postives = 336/517 (64.99%), Query Frame = 0

Query: 26  LDVQILEITFALFVFLVIHVVRQKRRH-GLPIWPIVGMLPALITATRGNLYEWITDLLCH 85
           +D     I   L V  +I  V  +R + G  +WP+VG LPALIT     ++++I D L  
Sbjct: 1   MDGSTAAIILTLIVTYIIWFVSLRRSYKGPRVWPLVGSLPALITNAH-RMHDFIADNLRM 60

Query: 86  HKGTFQ---FRGPWLTSL--NCVVTADPRNLEHLLKTKFSSYPKGSFFRDTVRDLLGDGI 145
             GT+Q   F  P+L     +  VT DP+NLEH+LKT+F +YPKG  ++    DLLGDGI
Sbjct: 61  CGGTYQTCIFPIPFLAKKQGHVTVTCDPKNLEHILKTRFDNYPKGPSWQSVFHDLLGDGI 120

Query: 146 FGADDETWQRQRKVASIEFHSARFRQMTVESLVELVHSRLEAVLEDFEGQSTTVDLQDVL 205
           F +D +TW+ QRK A++EF +   RQ     +   + +RL  +LE    ++  +DLQDVL
Sbjct: 121 FNSDGDTWRFQRKTAALEFTTRTLRQAMARWVDRAIKNRLVPILESARSRAEPIDLQDVL 180

Query: 206 LRLTFDNVCMIAFGVDPGCLRSGLPEIPFAKAFEDATEVTVIRFITPTFIWKFMRYLNIG 265
           LRLTFDN+C + FG DP  L    PE  FA AF+ ATE T+ RFI P FIWK  ++L +G
Sbjct: 181 LRLTFDNICGLTFGKDPRTLSPEFPENGFAVAFDGATEATLQRFIMPEFIWKIRKWLRLG 240

Query: 266 IEKKLKQSIKAVDEFAEDVIQTRKRELSTPAESQAENQRSDLLTVFMKLRDEQGRPFSDK 325
           +E  + +SI  VD +  ++I TRK EL    + Q E++  DLL+ FMK ++     +SDK
Sbjct: 241 LEDDMSRSISHVDNYLSEIINTRKLELL--GQQQDESRHDDLLSRFMKKKES----YSDK 300

Query: 326 FLRDICVNFILAGRDTSSVALSWFFLLLDQNPDVEDKILEEICRIVGEREEAKTRELRFD 385
           +L+ + +NFILAGRDTSSVA+SWFF L+  NP VE+KI+ EIC I+ +  +    +   +
Sbjct: 301 YLKYVALNFILAGRDTSSVAMSWFFWLVSLNPRVEEKIINEICTILIKTRDTNVSKWTDE 360

Query: 386 SLVFKPEEIKKMEYLHAAISEALRLYPSVPFDHKEVVEDDVFPDGTVLKKGTKIIYAIYA 445
            L F  +EI ++ YL AA+SE LRLYPSVP D K VV +DV PDGT +  G+ + Y+IY+
Sbjct: 361 PLTF--DEIDQLVYLKAALSETLRLYPSVPEDSKFVVANDVLPDGTFVPSGSNVTYSIYS 420

Query: 446 MGRMEAIWGKDCREFKPERWMREGRFMSESAYRFTAFNGGPRLCLGKDFAYYQMKYVAAS 505
           +GRM+ IWG+DC EFKPERW+ E R    + Y+F AFN GPR+CLGKD AY QMK + AS
Sbjct: 421 VGRMKFIWGEDCLEFKPERWLEESRDEKCNQYKFVAFNAGPRICLGKDLAYLQMKSITAS 480

Query: 506 IISRYRVAVVRGHPVEPKLALTFYMKKGLKVNLIKRE 537
           I+ R+R+ V  GH VE K++LT +MK GLK+++ KR+
Sbjct: 481 ILLRHRLTVAPGHRVEQKMSLTLFMKFGLKMDVHKRD 508

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FMY11.2e-22070.02Cytochrome P450 86B1 OS=Arabidopsis thaliana OX=3702 GN=CYP86B1 PE=2 SV=1[more]
Q9CAD61.5e-12747.00Cytochrome P450 86A7 OS=Arabidopsis thaliana OX=3702 GN=CYP86A7 PE=2 SV=1[more]
B3RFJ61.4e-12547.87Cytochrome P450 86A22 OS=Petunia hybrida OX=4102 GN=CYP86A22 PE=1 SV=1[more]
Q9LMM11.6e-12448.17Cytochrome P450 86A4 OS=Arabidopsis thaliana OX=3702 GN=CYP86A4 PE=1 SV=1[more]
O230665.0e-12346.64Cytochrome P450 86A2 OS=Arabidopsis thaliana OX=3702 GN=CYP86A2 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0K8790.0e+0098.73Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G420770 PE=3 SV=1[more]
A0A5A7UTT48.2e-30294.73Cytochrome P450 86B1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... [more]
A0A1S3CEY89.1e-30194.36LOW QUALITY PROTEIN: cytochrome P450 86B1-like OS=Cucumis melo OX=3656 GN=LOC103... [more]
A0A6J1GQ361.1e-28790.15cytochrome P450 86B1-like OS=Cucurbita moschata OX=3662 GN=LOC111456469 PE=3 SV=... [more]
A0A6J1JMG11.9e-28288.87cytochrome P450 86B1-like OS=Cucurbita maxima OX=3661 GN=LOC111488206 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_004135954.30.0e+0098.73cytochrome P450 86B1 [Cucumis sativus] >KAE8646384.1 hypothetical protein Csa_01... [more]
KAA0058578.11.7e-30194.73cytochrome P450 86B1-like [Cucumis melo var. makuwa] >TYK10378.1 cytochrome P450... [more]
XP_008461361.11.9e-30094.36PREDICTED: LOW QUALITY PROTEIN: cytochrome P450 86B1-like [Cucumis melo][more]
XP_022954102.12.4e-28790.15cytochrome P450 86B1-like [Cucurbita moschata][more]
KAG7014270.16.9e-28789.78Cytochrome P450 86B1, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
AT5G23190.18.3e-22270.02cytochrome P450, family 86, subfamily B, polypeptide 1 [more]
AT5G08250.11.7e-20669.98Cytochrome P450 superfamily protein [more]
AT1G24540.11.1e-13846.62cytochrome P450, family 86, subfamily C, polypeptide 1 [more]
AT1G13140.18.8e-13144.77cytochrome P450, family 86, subfamily C, polypeptide 3 [more]
AT1G63710.11.1e-12847.00cytochrome P450, family 86, subfamily A, polypeptide 7 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 473..483
score: 42.0
coord: 320..337
score: 28.15
coord: 395..413
score: 31.1
coord: 483..506
score: 19.46
coord: 340..366
score: 36.53
IPR001128Cytochrome P450PRINTSPR00385P450coord: 474..483
score: 46.03
coord: 483..494
score: 35.08
coord: 331..348
score: 39.78
coord: 396..407
score: 44.94
IPR001128Cytochrome P450PFAMPF00067p450coord: 54..520
e-value: 1.7E-79
score: 267.8
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 49..545
e-value: 1.0E-106
score: 359.2
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 53..535
NoneNo IPR availablePANTHERPTHR24296:SF177CYTOCHROME P450 86B1-LIKEcoord: 19..536
NoneNo IPR availablePANTHERPTHR24296CYTOCHROME P450coord: 19..536
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 476..485

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI07G18810.1CSPI07G18810.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen