CSPI07G18190 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI07G18190
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Protein kinase domain-containing protein
Location	Chr7: 16292147 .. 16293208 (+)
RNA-Seq Expression	CSPI07G18190
Synteny	CSPI07G18190

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATCAGGATTGGGTTCTAGTGAAGGCTTTAGGCGAAGGTTCTTGTGGCTTAGTTTGCTTAGCAAAACAAATCACTAAAGAAGAATCTGATCTTCATTATTATTTTGCTGTCAAGCGTGCTTCTCTTCGATATAATTCTTCTTCATTGTTGCGGGAAGAACATGTCTTGAAACATTTCACGGATTGTCCAGAAATTGTTCAATATCTAGGAAGTGAGGTAACTGGAGGAGGAGATTTTCTTGATGATATGGAACTTTACAATTTGAAGCTAGAGTATGCTGCTGGAGGGACATTAGCTGATTTGATCAAACAAAGAAATAAGTTACCTGAGGATGAGGTGAAGAAGTATCTTCAAATGATTCTTAAAGGCCTTTCATGTATTCATCGTAAAGGATTTGTTCATGTCGATCTGAAGCCTGATAATATTCTTGCCTTTCCTCAAAGTGATGGAAAGATGAAATTGAAAATTGCTGACTTTGGACTTGCTGAGAGATCATGTAAACGTGGGGAGGATGATCAAGAAGACAGAGGGAGCAAGTACTACTCTGGCGCGTTGAAGGTTAGAGCAACACATAGATACATGTCACCTGAATCCATCGTTTTCAGTGAGATCAATGGTTTGCATGATATTTGGTCGTTGGGTTGCACTTTGGTTCAAATGGTTTCTGGAGAGCGAGTGTGGAATGATTGCAAAAGTTATGAAGAATTAATAACAAAACTTTTGATTAGCGAAGAAATACCAACAATACCTGAGGAATTATCCAAACAAGGTAAAGATTTTCTTGAAAATTGTTTTGTTCGAAACTATGAACAACGGTGGACAGCTGATATGCTACTCCAACATCCCTACCTCAATGAAGAAAATAAAGATACGAAGAACGGTGATGAGAAGCTTAAACTTCCAAAGGCCATAGTTTTTTTGCCTCATCAATTCTTTCAGAGCAAGACTGATATGCTACTCCAACAGACGAAGAACGGTGATGAGAAGCTTAAACCTTCAAAAGCCATTTTTTTGCCTCATCAATTCTTCAAGAGCAAGGCTCAATCTTGTAATAATTAG

mRNA sequence

ATGGATCAGGATTGGGTTCTAGTGAAGGCTTTAGGCGAAGGTTCTTGTGGCTTAGTTTGCTTAGCAAAACAAATCACTAAAGAAGAATCTGATCTTCATTATTATTTTGCTGTCAAGCGTGCTTCTCTTCGATATAATTCTTCTTCATTGTTGCGGGAAGAACATGTCTTGAAACATTTCACGGATTGTCCAGAAATTGTTCAATATCTAGGAAGTGAGGTAACTGGAGGAGGAGATTTTCTTGATGATATGGAACTTTACAATTTGAAGCTAGAGTATGCTGCTGGAGGGACATTAGCTGATTTGATCAAACAAAGAAATAAGTTACCTGAGGATGAGGTGAAGAAGTATCTTCAAATGATTCTTAAAGGCCTTTCATGTATTCATCGTAAAGGATTTGTTCATGTCGATCTGAAGCCTGATAATATTCTTGCCTTTCCTCAAAGTGATGGAAAGATGAAATTGAAAATTGCTGACTTTGGACTTGCTGAGAGATCATGTAAACGTGGGGAGGATGATCAAGAAGACAGAGGGAGCAAGTACTACTCTGGCGCGTTGAAGGTTAGAGCAACACATAGATACATGTCACCTGAATCCATCGTTTTCAGTGAGATCAATGGTTTGCATGATATTTGGTCGTTGGGTTGCACTTTGGTTCAAATGGTTTCTGGAGAGCGAGTGTGGAATGATTGCAAAAGTTATGAAGAATTAATAACAAAACTTTTGATTAGCGAAGAAATACCAACAATACCTGAGGAATTATCCAAACAAGGTAAAGATTTTCTTGAAAATTGTTTTGTTCGAAACTATGAACAACGGTGGACAGCTGATATGCTACTCCAACATCCCTACCTCAATGAAGAAAATAAAGATACGAAGAACGGTGATGAGAAGCTTAAACTTCCAAAGGCCATAGTTTTTTTGCCTCATCAATTCTTTCAGAGCAAGACTGATATGCTACTCCAACAGACGAAGAACGGTGATGAGAAGCTTAAACCTTCAAAAGCCATTTTTTTGCCTCATCAATTCTTCAAGAGCAAGGCTCAATCTTGTAATAATTAG

Coding sequence (CDS)

ATGGATCAGGATTGGGTTCTAGTGAAGGCTTTAGGCGAAGGTTCTTGTGGCTTAGTTTGCTTAGCAAAACAAATCACTAAAGAAGAATCTGATCTTCATTATTATTTTGCTGTCAAGCGTGCTTCTCTTCGATATAATTCTTCTTCATTGTTGCGGGAAGAACATGTCTTGAAACATTTCACGGATTGTCCAGAAATTGTTCAATATCTAGGAAGTGAGGTAACTGGAGGAGGAGATTTTCTTGATGATATGGAACTTTACAATTTGAAGCTAGAGTATGCTGCTGGAGGGACATTAGCTGATTTGATCAAACAAAGAAATAAGTTACCTGAGGATGAGGTGAAGAAGTATCTTCAAATGATTCTTAAAGGCCTTTCATGTATTCATCGTAAAGGATTTGTTCATGTCGATCTGAAGCCTGATAATATTCTTGCCTTTCCTCAAAGTGATGGAAAGATGAAATTGAAAATTGCTGACTTTGGACTTGCTGAGAGATCATGTAAACGTGGGGAGGATGATCAAGAAGACAGAGGGAGCAAGTACTACTCTGGCGCGTTGAAGGTTAGAGCAACACATAGATACATGTCACCTGAATCCATCGTTTTCAGTGAGATCAATGGTTTGCATGATATTTGGTCGTTGGGTTGCACTTTGGTTCAAATGGTTTCTGGAGAGCGAGTGTGGAATGATTGCAAAAGTTATGAAGAATTAATAACAAAACTTTTGATTAGCGAAGAAATACCAACAATACCTGAGGAATTATCCAAACAAGGTAAAGATTTTCTTGAAAATTGTTTTGTTCGAAACTATGAACAACGGTGGACAGCTGATATGCTACTCCAACATCCCTACCTCAATGAAGAAAATAAAGATACGAAGAACGGTGATGAGAAGCTTAAACTTCCAAAGGCCATAGTTTTTTTGCCTCATCAATTCTTTCAGAGCAAGACTGATATGCTACTCCAACAGACGAAGAACGGTGATGAGAAGCTTAAACCTTCAAAAGCCATTTTTTTGCCTCATCAATTCTTCAAGAGCAAGGCTCAATCTTGTAATAATTAG

Protein sequence

MDQDWVLVKALGEGSCGLVCLAKQITKEESDLHYYFAVKRASLRYNSSSLLREEHVLKHFTDCPEIVQYLGSEVTGGGDFLDDMELYNLKLEYAAGGTLADLIKQRNKLPEDEVKKYLQMILKGLSCIHRKGFVHVDLKPDNILAFPQSDGKMKLKIADFGLAERSCKRGEDDQEDRGSKYYSGALKVRATHRYMSPESIVFSEINGLHDIWSLGCTLVQMVSGERVWNDCKSYEELITKLLISEEIPTIPEELSKQGKDFLENCFVRNYEQRWTADMLLQHPYLNEENKDTKNGDEKLKLPKAIVFLPHQFFQSKTDMLLQQTKNGDEKLKPSKAIFLPHQFFKSKAQSCNN*

Homology

[Close]

BLAST of CSPI07G18190 vs. ExPASy Swiss-Prot
Match: Q9SND6 (Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK20 PE=1 SV=1)

HSP 1 Score: 182.6 bits (462), Expect = 7.9e-45
Identity = 112/313 (35.78%), Postives = 173/313 (55.27%), Query Frame = 0


Query: 4   DWVLVKALGEGSCGLVCLAKQITKEESDLHYYFAVKRASLRYNSSSLLREEHVLKHFTDC 63
           +WV  + +G G+   V  A + ++   D     AVK     Y ++SL  E+ VL    DC
Sbjct: 2   EWVRGETIGFGTFSTVSTATK-SRNSGDFPALIAVKSTD-AYGAASLSNEKSVLDSLGDC 61

Query: 64  PEIVQYLGSEVTGGGDFLDDMELYNLKLEYAAGGTLADLIKQ--RNKLPEDEVKKYLQMI 123
           PEI++  G + T      +  E++NL LEYA+ G+LA  +K+     LPE  V+++   +
Sbjct: 62  PEIIRCYGEDST----VENGEEMHNLLLEYASRGSLASYMKKLGGEGLPESTVRRHTGSV 121

Query: 124 LKGLSCIHRKGFVHVDLKPDNILAFPQSDGKMKLKIADFGLAERSCKRGEDDQEDRGSKY 183
           L+GL  IH KGF H D+K  NIL F  +DG   +KIADFGLA R          D     
Sbjct: 122 LRGLRHIHAKGFAHCDIKLANILLF--NDG--SVKIADFGLAMR---------VDGDLTA 181

Query: 184 YSGALKVRATHRYMSPESIVFSEINGLHDIWSLGCTLVQMVSGERVWN--DCKSYEELIT 243
              ++++R T  YM+PE +  +E     D+W+LGC +V+M SG+  W+  +   +  L+ 
Sbjct: 182 LRKSVEIRGTPLYMAPECVNDNEYGSAADVWALGCAVVEMFSGKTAWSVKEGSHFMSLLI 241

Query: 244 KLLISEEIPTIPEELSKQGKDFLENCFVRNYEQRWTADMLLQHPYLNEENKD-------- 303
           ++ + +E+P IPE LS++GKDFL  CFV++  +RWTA+MLL H ++  + +D        
Sbjct: 242 RIGVGDELPKIPEMLSEEGKDFLSKCFVKDPAKRWTAEMLLNHSFVTIDLEDDHRENFVV 295

[Close]

BLAST of CSPI07G18190 vs. ExPASy Swiss-Prot
Match: O80888 (Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK17 PE=1 SV=1)

HSP 1 Score: 145.2 bits (365), Expect = 1.4e-33
Identity = 99/286 (34.62%), Postives = 139/286 (48.60%), Query Frame = 0


Query: 4   DWVLVKALGEGSCGLVCLAKQITKEESDLHYYFAVKRASLRYNSSSLLREEHVLKHFTDC 63
           +W   + LG GS   V  A     +E       AVK + + + S  L RE  +L   +  
Sbjct: 2   EWTRGRILGRGSTATVYAAAGHNSDE-----ILAVKSSEV-HRSEFLQREAKILSSLSS- 61

Query: 64  PEIVQYLGSEVTGGGDFLDDMELYNLKLEYAAGGTLAD-LIKQRNKLPEDEVKKYLQMIL 123
           P ++ Y GSE        + + +YNL +EYA  GTL D   K   ++ E  V KY + IL
Sbjct: 62  PYVIGYRGSETKRES---NGVVMYNLLMEYAPYGTLTDAAAKDGGRVDETRVVKYTRDIL 121

Query: 124 KGLSCIHRKGFVHVDLKPDNILAFPQSDGKMKLKIADFGLAERSCKRGEDDQEDRGSKYY 183
           KGL  IH KG VH D+K  N++       K + KIADFG A+R     E           
Sbjct: 122 KGLEYIHSKGIVHCDVKGSNVVI----SEKGEAKIADFGCAKRVDPVFES---------- 181

Query: 184 SGALKVRATHRYMSPESIVFSEINGLHDIWSLGCTLVQMVSGERVWNDCKSYEELITKLL 243
                V  T  +M+PE     +     DIW++GCT+++MV+G   W    S E+ ++ L 
Sbjct: 182 ----PVMGTPAFMAPEVARGEKQGKESDIWAVGCTMIEMVTGSPPWTKADSREDPVSVLY 241

Query: 244 ---ISEEIPTIPEELSKQGKDFLENCFVRNYEQRWTADMLLQHPYL 286
               S E P +P  L+++ KDFLE C  R   +RWTA  LL HP+L
Sbjct: 242 RVGYSSETPELPCLLAEEAKDFLEKCLKREANERWTATQLLNHPFL 259

[Close]

BLAST of CSPI07G18190 vs. ExPASy Swiss-Prot
Match: Q01389 (Serine/threonine-protein kinase BCK1/SLK1/SSP31 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=BCK1 PE=1 SV=1)

HSP 1 Score: 136.0 bits (341), Expect = 8.5e-31
Identity = 96/294 (32.65%), Postives = 152/294 (51.70%), Query Frame = 0


Query: 5    WVLVKALGEGSCGLVCLAKQITKEESDLHYYFAVKRASL-RYNSSS--------LLREEH 64
            W+  + +G+GS G V L   +T  E       AVK+  + +Y+S +         LR E 
Sbjct: 1175 WMKGEMIGKGSFGAVYLCLNVTTGE-----MMAVKQVEVPKYSSQNEAILSTVEALRSEV 1234

Query: 65   VLKHFTDCPEIVQYLGSEVTGGGDFLDDMELYNLKLEYAAGGTLADLIKQRNKLPEDEVK 124
                  D   IVQYLG        F +   +Y+L LEY AGG++  LI+   +  E  +K
Sbjct: 1235 STLKDLDHLNIVQYLG--------FENKNNIYSLFLEYVAGGSVGSLIRMYGRFDEPLIK 1294

Query: 125  KYLQMILKGLSCIHRKGFVHVDLKPDNILAFPQSDGKMKLKIADFGLAERSCKRGEDDQE 184
                 +LKGL+ +H KG +H D+K DN+L     DG    KI+DFG++ +S         
Sbjct: 1295 HLTTQVLKGLAYLHSKGILHRDMKADNLLL--DQDG--ICKISDFGISRKS--------- 1354

Query: 185  DRGSKYYSGALKVRATHRYMSPESIVFSE-INGLHDIWSLGCTLVQMVSGERVWNDCKSY 244
                 Y +  + +R T  +M+PE +   +  +   DIWSLGC +++M +G+R W++ +  
Sbjct: 1355 --KDIYSNSDMTMRGTVFWMAPEMVDTKQGYSAKVDIWSLGCIVLEMFAGKRPWSNLEVV 1414

Query: 245  EELITKLLISEEIPTIPEE----LSKQGKDFLENCFVRNYEQRWTADMLLQHPY 285
              +  K+  S+  P IPE+    +S+ G++FL+ CF  N E+R TA+ LL HP+
Sbjct: 1415 AAMF-KIGKSKSAPPIPEDTLPLISQIGRNFLDACFEINPEKRPTANELLSHPF 1439

[Close]

BLAST of CSPI07G18190 vs. ExPASy Swiss-Prot
Match: Q40541 (Mitogen-activated protein kinase kinase kinase NPK1 OS=Nicotiana tabacum OX=4097 GN=NPK1 PE=1 SV=1)

HSP 1 Score: 136.0 bits (341), Expect = 8.5e-31
Identity = 88/301 (29.24%), Postives = 152/301 (50.50%), Query Frame = 0


Query: 5   WVLVKALGEGSCGLVCLAKQITKEESDLHYYFAVKRASLRYNSSS----------LLREE 64
           W   + +G G+ G V +   +   E       A+K  S+  N +S          L  E 
Sbjct: 80  WRKGEMIGCGAFGRVYMGMNVDSGE-----LLAIKEVSIAMNGASRERAQAHVRELEEEV 139

Query: 65  HVLKHFTDCPEIVQYLGSEVTGGGDFLDDMELYNLKLEYAAGGTLADLIKQRNKLPEDEV 124
           ++LK+ +  P IV+YLG+    G          N+ LE+  GG+++ L+ +    PE  +
Sbjct: 140 NLLKNLSH-PNIVRYLGTAREAGS--------LNILLEFVPGGSISSLLGKFGSFPESVI 199

Query: 125 KKYLQMILKGLSCIHRKGFVHVDLKPDNILAFPQSDGKMKLKIADFGLAERSCKRGEDDQ 184
           + Y + +L GL  +H+ G +H D+K  NIL     D K  +K+ADFG +++  +      
Sbjct: 200 RMYTKQLLLGLEYLHKNGIMHRDIKGANILV----DNKGCIKLADFGASKKVVELAT--- 259

Query: 185 EDRGSKYYSGALKVRATHRYMSPESIVFSEINGLHDIWSLGCTLVQMVSGERVWNDCKSY 244
                   +GA  ++ T  +M+PE I+ +  +   DIWS+GCT+++M +G+  W+  + Y
Sbjct: 260 -------MTGAKSMKGTPYWMAPEVILQTGHSFSADIWSVGCTIIEMATGKPPWS--QQY 319

Query: 245 EELITKLLI--SEEIPTIPEELSKQGKDFLENCFVRNYEQRWTADMLLQHPYLNEENKDT 294
           +E+     I  ++  P IPE LS + KDFL  C  +    R +A  LLQHP++  E+++ 
Sbjct: 320 QEVAALFHIGTTKSHPPIPEHLSAESKDFLLKCLQKEPHLRHSASNLLQHPFVTAEHQEA 350

[Close]

BLAST of CSPI07G18190 vs. ExPASy Swiss-Prot
Match: Q9CAD5 (Mitogen-activated protein kinase kinase kinase YODA OS=Arabidopsis thaliana OX=3702 GN=YDA PE=1 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 1.5e-30
Identity = 96/289 (33.22%), Postives = 147/289 (50.87%), Query Frame = 0


Query: 5   WVLVKALGEGSCGLVCLAKQITKEESDLHYYFAVKRASL-------RYNSSSLLREEHVL 64
           W   + LG GS G V L      E  ++    A+K  +L       R ++  L +E  VL
Sbjct: 400 WKKGRLLGMGSFGHVYLG--FNSESGEM---CAMKEVTLCSDDPKSRESAQQLGQEISVL 459

Query: 65  KHFTDCPEIVQYLGSEVTGGGDFLDDMELYNLKLEYAAGGTLADLIKQRNKLPEDEVKKY 124
                   IVQY GSE         D +LY + LEY +GG++  L+++  +  E+ ++ Y
Sbjct: 460 SRLRH-QNIVQYYGSETV-------DDKLY-IYLEYVSGGSIYKLLQEYGQFGENAIRNY 519

Query: 125 LQMILKGLSCIHRKGFVHVDLKPDNILAFPQSDGKMKLKIADFGLAERSCKRGEDDQEDR 184
            Q IL GL+ +H K  VH D+K  NIL  P      ++K+ADFG+A+    +        
Sbjct: 520 TQQILSGLAYLHAKNTVHRDIKGANILVDPHG----RVKVADFGMAKHITAQ-------- 579

Query: 185 GSKYYSGALKVRATHRYMSPESIVFSEINGLH-DIWSLGCTLVQMVSGERVWNDCKSYEE 244
                SG L  + +  +M+PE I  S  + L  DIWSLGCT+++M + +  W+  +    
Sbjct: 580 -----SGPLSFKGSPYWMAPEVIKNSNGSNLAVDIWSLGCTVLEMATTKPPWSQYEGVPA 639

Query: 245 LITKLLISEEIPTIPEELSKQGKDFLENCFVRNYEQRWTADMLLQHPYL 286
           +  K+  S+E+P IP+ LS++GKDF+  C  RN   R TA  LL H ++
Sbjct: 640 MF-KIGNSKELPDIPDHLSEEGKDFVRKCLQRNPANRPTAAQLLDHAFV 656

[Close]

BLAST of CSPI07G18190 vs. ExPASy TrEMBL
Match: A0A0A0K835 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G407720 PE=4 SV=1)

HSP 1 Score: 721.1 bits (1860), Expect = 2.3e-204
Identity = 350/353 (99.15%), Postives = 350/353 (99.15%), Query Frame = 0


Query: 1   MDQDWVLVKALGEGSCGLVCLAKQITKEESDLHYYFAVKRASLRYNSSSLLREEHVLKHF 60
           MDQDWVLVKALGEGSCGLVCLAKQITKEESDLHYYFAVKRASLRYNSSSLL EEHVLKHF
Sbjct: 1   MDQDWVLVKALGEGSCGLVCLAKQITKEESDLHYYFAVKRASLRYNSSSLLWEEHVLKHF 60

Query: 61  TDCPEIVQYLGSEVTGGGDFLDDMELYNLKLEYAAGGTLADLIKQRNKLPEDEVKKYLQM 120
           TDCPEIVQYLGSEVTGGGDFLDD ELYNLKLEYAAGGTLADLIKQRNKLPEDEVKKYLQM
Sbjct: 61  TDCPEIVQYLGSEVTGGGDFLDDKELYNLKLEYAAGGTLADLIKQRNKLPEDEVKKYLQM 120

Query: 121 ILKGLSCIHRKGFVHVDLKPDNILAFPQSDGKMKLKIADFGLAERSCKRGEDDQEDRGSK 180
           ILKGLSCIHRKGFVHVDLKPDNILAFPQSDGKMKLKIADFGLAERSCKRGEDDQEDRGSK
Sbjct: 121 ILKGLSCIHRKGFVHVDLKPDNILAFPQSDGKMKLKIADFGLAERSCKRGEDDQEDRGSK 180

Query: 181 YYSGALKVRATHRYMSPESIVFSEINGLHDIWSLGCTLVQMVSGERVWNDCKSYEELITK 240
           YYSGALKVRATHRYMSPESIVFSEINGLHDIWSLGCTLVQMVSGERVWNDCKSYEELITK
Sbjct: 181 YYSGALKVRATHRYMSPESIVFSEINGLHDIWSLGCTLVQMVSGERVWNDCKSYEELITK 240

Query: 241 LLISEEIPTIPEELSKQGKDFLENCFVRNYEQRWTADMLLQHPYLNEENKDTKNGDEKLK 300
           LLISEEIPTIPEELSKQGKDFLE CFVRNYEQRWTADMLLQHPYLNEENKDTKNGDEKLK
Sbjct: 241 LLISEEIPTIPEELSKQGKDFLEKCFVRNYEQRWTADMLLQHPYLNEENKDTKNGDEKLK 300

Query: 301 LPKAIVFLPHQFFQSKTDMLLQQTKNGDEKLKPSKAIFLPHQFFKSKAQSCNN 354
           LPKAIVFLPHQFFQSKTDMLLQQTKNGDEKLKPSKAIFLPHQFFKSKAQSCNN
Sbjct: 301 LPKAIVFLPHQFFQSKTDMLLQQTKNGDEKLKPSKAIFLPHQFFKSKAQSCNN 353

[Close]

BLAST of CSPI07G18190 vs. ExPASy TrEMBL
Match: A0A5A7TZP4 (Serine/threonine-protein kinase Chk1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G001750 PE=4 SV=1)

HSP 1 Score: 599.7 bits (1545), Expect = 7.7e-168
Identity = 302/353 (85.55%), Postives = 320/353 (90.65%), Query Frame = 0


Query: 3   QDWVLVKALGEGSCGLVCLAKQITKEESDLHYYFAVKRASLRYNSSSLLREEHVLKHFTD 62
           +DWVL+K LGEGSCGLVCLAKQITKEESDLHYYFAVKRASLRYNS+SLL EEHVLKH TD
Sbjct: 2   EDWVLLKVLGEGSCGLVCLAKQITKEESDLHYYFAVKRASLRYNSASLLWEEHVLKHLTD 61

Query: 63  CPEIVQYLGSEVTGGGDFLDDMELYNLKLEYAAGGTLADLIKQRNKLPEDEVKKYLQMIL 122
           CPEIVQYLGSEVTGGGDFLDD ELYNLKLEYAAGGTLADLIKQRNKLPEDEVKKYLQMIL
Sbjct: 62  CPEIVQYLGSEVTGGGDFLDDKELYNLKLEYAAGGTLADLIKQRNKLPEDEVKKYLQMIL 121

Query: 123 KGLSCIHRKGFVHVDLKPDNILAFPQSDGKMKLKIADFGLAERSCKRGEDDQEDRGSKYY 182
           KGLSCIHRKGFVHVDLKPDNILAFPQSDGKMKLKIADFGLAER  K GEDDQED+G ++Y
Sbjct: 122 KGLSCIHRKGFVHVDLKPDNILAFPQSDGKMKLKIADFGLAER-FKHGEDDQEDKGCEHY 181

Query: 183 SGALKVRATHRYMSPESIVFSEINGLHDIWSLGCTLVQMVSGERVWNDCKSYEELITKLL 242
            GAL+VRATHRYMSPESIV  EI+GLHDIWSLGC LVQMVSGERVWNDCK+YEEL+TKLL
Sbjct: 182 -GALRVRATHRYMSPESIVLKEIDGLHDIWSLGCVLVQMVSGERVWNDCKNYEELMTKLL 241

Query: 243 ISEEIPTIPEELSKQGKDFLENCFVRNYEQRWTADMLLQHPYLNEENKDTKNGDEKLKLP 302
            S+EIP IP+ELSKQG DFLE CFVR+Y+QRWTADMLLQHPYLNE+NKDT + DEKLKLP
Sbjct: 242 NSKEIPIIPKELSKQGIDFLEKCFVRDYKQRWTADMLLQHPYLNEQNKDTSD-DEKLKLP 301

Query: 303 KAIVFLPHQFFQSKTDMLLQQTKNGDEKLK-PSKAIFLPHQFFKSKA-QSCNN 354
           K   FLPHQFF  K DML    KNGDEKL+ P KAIFLPHQFF S A QSCN+
Sbjct: 302 KT-AFLPHQFFNRKADML----KNGDEKLELPKKAIFLPHQFFNSTAQQSCND 346

[Close]

BLAST of CSPI07G18190 vs. ExPASy TrEMBL
Match: A0A1S3BRE6 (serine/threonine-protein kinase Chk1-like OS=Cucumis melo OX=3656 GN=LOC103492884 PE=4 SV=1)

HSP 1 Score: 466.5 bits (1199), Expect = 1.0e-127
Identity = 229/255 (89.80%), Postives = 241/255 (94.51%), Query Frame = 0


Query: 3   QDWVLVKALGEGSCGLVCLAKQITKEESDLHYYFAVKRASLRYNSSSLLREEHVLKHFTD 62
           +DWVL+K LGEGSCGLVCLAKQITKEESDLHYYFAVKRASLRYNS+SLL EEHVLKH TD
Sbjct: 2   EDWVLLKVLGEGSCGLVCLAKQITKEESDLHYYFAVKRASLRYNSASLLWEEHVLKHLTD 61

Query: 63  CPEIVQYLGSEVTGGGDFLDDMELYNLKLEYAAGGTLADLIKQRNKLPEDEVKKYLQMIL 122
           CPEIVQYLGSEVTGGGDFLDD ELYNLKLEYAAGGTLADLIKQRNKLPEDEVKKYLQMIL
Sbjct: 62  CPEIVQYLGSEVTGGGDFLDDKELYNLKLEYAAGGTLADLIKQRNKLPEDEVKKYLQMIL 121

Query: 123 KGLSCIHRKGFVHVDLKPDNILAFPQSDGKMKLKIADFGLAERSCKRGEDDQEDRGSKYY 182
           KGLSCIHRKGFVHVDLKPDNILAFPQSDGKMKLKIADFGLAER  K GEDDQED+G ++Y
Sbjct: 122 KGLSCIHRKGFVHVDLKPDNILAFPQSDGKMKLKIADFGLAER-FKHGEDDQEDKGCEHY 181

Query: 183 SGALKVRATHRYMSPESIVFSEINGLHDIWSLGCTLVQMVSGERVWNDCKSYEELITKLL 242
            GAL+VRATHRYMSPESIV  EI+GLHDIWSLGC LVQMVSGERVWNDCK+YEEL+TKLL
Sbjct: 182 -GALRVRATHRYMSPESIVLKEIDGLHDIWSLGCVLVQMVSGERVWNDCKNYEELMTKLL 241

Query: 243 ISEEIPTIPEELSKQ 258
            S+EIP IP+ELSKQ
Sbjct: 242 NSKEIPIIPKELSKQ 254

[Close]

BLAST of CSPI07G18190 vs. ExPASy TrEMBL
Match: A0A0A0K7G3 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G065150 PE=4 SV=1)

HSP 1 Score: 384.8 bits (987), Expect = 3.9e-103
Identity = 202/321 (62.93%), Postives = 247/321 (76.95%), Query Frame = 0


Query: 3   QDWVLVKALGEGSCGLVCLAKQITKEE--SDLHYYFAVKRASLRYNSSSLLREEHVLKHF 62
           ++WVLVK LG+GS GLVCLAKQ T+++  ++LHYYFAVK + L +NSS  L EE VLK F
Sbjct: 2   ENWVLVKILGQGSDGLVCLAKQTTRKKIYNNLHYYFAVKLSPLGHNSS--LWEELVLKQF 61

Query: 63  TDCPEIVQYLGSEVT---GGGDFLDDMELYNLKLEYAAGGTLADLIKQRNKLPEDEVKKY 122
            +CPEIVQYLGSE+T   GG D   D + Y LKLEYAAGGTL DLIKQ+ KLPEDEVK+Y
Sbjct: 62  KNCPEIVQYLGSEITRSPGGRDLPGDKDFYTLKLEYAAGGTLDDLIKQKEKLPEDEVKEY 121

Query: 123 LQMILKGLSCIHRKGFVHVDLKPDNILAFPQSDGKMKLKIADFGLAERSCKRGED----- 182
           L+MILKGLSCIH KGFVHVDLKP NILAFP+S+GKMKLKIADFG AER CK   D     
Sbjct: 122 LRMILKGLSCIHSKGFVHVDLKPKNILAFPRSNGKMKLKIADFGHAER-CKYRNDADAHS 181

Query: 183 DQEDRGSKYYSGALKVRATHRYMSPESIVFSEINGLHDIWSLGCTLVQMVSGERVWNDCK 242
           D E R S+  S  LK + T RYMSPESIVF E+N  HD+WSLGC LV+M+SG+  W++  
Sbjct: 182 DDEYRKSRRRSSLLKFKGTPRYMSPESIVFDEVNDAHDVWSLGCVLVRMISGKSAWDEVA 241

Query: 243 SYEELITKLLISEEIPTIPEELSKQGKDFLENCFVRNYEQRWTADMLLQHPYLNEENKDT 302
           + ++L+ K+L S+ +P IPEELS QGKDF++ CFV N++QRWTADMLLQHPYLNE+++ T
Sbjct: 242 TSKQLMMKILDSKSMPRIPEELSIQGKDFIKKCFVWNFKQRWTADMLLQHPYLNEKHEAT 301

Query: 303 --KNGDEKLKLPKAIVFLPHQ 312
             K+ D   KL K+++ L HQ
Sbjct: 302 TMKDDDGMPKLAKSLL-LSHQ 318

[Close]

BLAST of CSPI07G18190 vs. ExPASy TrEMBL
Match: A0A5D3C4R6 (Mitogen-activated protein kinase kinase kinase A-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G002080 PE=4 SV=1)

HSP 1 Score: 382.5 bits (981), Expect = 1.9e-102
Identity = 206/351 (58.69%), Postives = 255/351 (72.65%), Query Frame = 0


Query: 3   QDWVLVKALGEGSCGLVCLAKQITKEE--SDLHYYFAVKRASLRYNSSSLLREEHVLKHF 62
           + WV VK LG+GS G+V LAKQ  + +   +LHYYFAVK + LR+N SSLL EE VLK F
Sbjct: 2   EKWVFVKVLGQGSDGVVWLAKQTIRRKIGRNLHYYFAVKLSPLRHN-SSLLWEEQVLKQF 61

Query: 63  TDCPEIVQYLGSEVTGGGDFLDDMELYNLKLEYAAGGTLADLIKQRNKLPEDEVKKYLQM 122
            +CPEIVQYLGSE+T GGD   D + Y LKLEYAAGGTL DLIKQ+ KLPE+EVK YL+M
Sbjct: 62  KNCPEIVQYLGSEITRGGDLPQDKDFYTLKLEYAAGGTLDDLIKQKEKLPENEVKDYLRM 121

Query: 123 ILKGLSCIHRKGFVHVDLKPDNILAFPQSDGKMKLKIADFGLAERSCK--RGEDDQEDRG 182
           ILKGLSC+H KGFVHVDLKP+NILAFPQSDGKMKLKIADFG AER CK  R +DD +D+ 
Sbjct: 122 ILKGLSCVHSKGFVHVDLKPNNILAFPQSDGKMKLKIADFGHAER-CKLYRNDDDDDDQD 181

Query: 183 SKY--YSGALKVRATHRYMSPESIVFSEINGLHDIWSLGCTLVQMVSGERVWNDCKSYEE 242
            KY  Y  +LK + T RYMSPESIV +E+N  HDIWSLGC LV+M+SG+ VW+D     +
Sbjct: 182 RKYGGYCSSLKFKGTPRYMSPESIVCNEVNDAHDIWSLGCVLVKMISGKSVWDDLIDSRQ 241

Query: 243 LITKLLISE--EIPTIPEELSKQGKDFLENCFVRNYEQRWTADMLLQHPYL-NEENK--D 302
           L+ +LL ++   +PTIP+ELSKQGKDF++ CF+RN +QRWTADMLLQHPYL +EEN+   
Sbjct: 242 LMMRLLDNKNYNMPTIPKELSKQGKDFIKKCFIRNCKQRWTADMLLQHPYLMHEENEAAT 301

Query: 303 TKNGDEKL--KLPKAIVFLPHQFFQSKTDMLLQQTKNGDEKLKPSKAIFLP 341
           T   D+++   L KA+     +  +S+T        N  +    +K   LP
Sbjct: 302 TVKDDDQMPTTLAKALSLSRQRPLKSQTRSSNNNNNNNRKFNLRAKNYLLP 350

[Close]

BLAST of CSPI07G18190 vs. NCBI nr
Match: XP_004136151.1 (mitogen-activated protein kinase kinase kinase 17 [Cucumis sativus] >KGN45019.1 hypothetical protein Csa_015671 [Cucumis sativus])

HSP 1 Score: 721.1 bits (1860), Expect = 4.7e-204
Identity = 350/353 (99.15%), Postives = 350/353 (99.15%), Query Frame = 0


Query: 1   MDQDWVLVKALGEGSCGLVCLAKQITKEESDLHYYFAVKRASLRYNSSSLLREEHVLKHF 60
           MDQDWVLVKALGEGSCGLVCLAKQITKEESDLHYYFAVKRASLRYNSSSLL EEHVLKHF
Sbjct: 1   MDQDWVLVKALGEGSCGLVCLAKQITKEESDLHYYFAVKRASLRYNSSSLLWEEHVLKHF 60

Query: 61  TDCPEIVQYLGSEVTGGGDFLDDMELYNLKLEYAAGGTLADLIKQRNKLPEDEVKKYLQM 120
           TDCPEIVQYLGSEVTGGGDFLDD ELYNLKLEYAAGGTLADLIKQRNKLPEDEVKKYLQM
Sbjct: 61  TDCPEIVQYLGSEVTGGGDFLDDKELYNLKLEYAAGGTLADLIKQRNKLPEDEVKKYLQM 120

Query: 121 ILKGLSCIHRKGFVHVDLKPDNILAFPQSDGKMKLKIADFGLAERSCKRGEDDQEDRGSK 180
           ILKGLSCIHRKGFVHVDLKPDNILAFPQSDGKMKLKIADFGLAERSCKRGEDDQEDRGSK
Sbjct: 121 ILKGLSCIHRKGFVHVDLKPDNILAFPQSDGKMKLKIADFGLAERSCKRGEDDQEDRGSK 180

Query: 181 YYSGALKVRATHRYMSPESIVFSEINGLHDIWSLGCTLVQMVSGERVWNDCKSYEELITK 240
           YYSGALKVRATHRYMSPESIVFSEINGLHDIWSLGCTLVQMVSGERVWNDCKSYEELITK
Sbjct: 181 YYSGALKVRATHRYMSPESIVFSEINGLHDIWSLGCTLVQMVSGERVWNDCKSYEELITK 240

Query: 241 LLISEEIPTIPEELSKQGKDFLENCFVRNYEQRWTADMLLQHPYLNEENKDTKNGDEKLK 300
           LLISEEIPTIPEELSKQGKDFLE CFVRNYEQRWTADMLLQHPYLNEENKDTKNGDEKLK
Sbjct: 241 LLISEEIPTIPEELSKQGKDFLEKCFVRNYEQRWTADMLLQHPYLNEENKDTKNGDEKLK 300

Query: 301 LPKAIVFLPHQFFQSKTDMLLQQTKNGDEKLKPSKAIFLPHQFFKSKAQSCNN 354
           LPKAIVFLPHQFFQSKTDMLLQQTKNGDEKLKPSKAIFLPHQFFKSKAQSCNN
Sbjct: 301 LPKAIVFLPHQFFQSKTDMLLQQTKNGDEKLKPSKAIFLPHQFFKSKAQSCNN 353

[Close]

BLAST of CSPI07G18190 vs. NCBI nr
Match: KAA0046699.1 (serine/threonine-protein kinase Chk1-like [Cucumis melo var. makuwa] >TYK18235.1 serine/threonine-protein kinase Chk1-like [Cucumis melo var. makuwa])

HSP 1 Score: 599.7 bits (1545), Expect = 1.6e-167
Identity = 302/353 (85.55%), Postives = 320/353 (90.65%), Query Frame = 0


Query: 3   QDWVLVKALGEGSCGLVCLAKQITKEESDLHYYFAVKRASLRYNSSSLLREEHVLKHFTD 62
           +DWVL+K LGEGSCGLVCLAKQITKEESDLHYYFAVKRASLRYNS+SLL EEHVLKH TD
Sbjct: 2   EDWVLLKVLGEGSCGLVCLAKQITKEESDLHYYFAVKRASLRYNSASLLWEEHVLKHLTD 61

Query: 63  CPEIVQYLGSEVTGGGDFLDDMELYNLKLEYAAGGTLADLIKQRNKLPEDEVKKYLQMIL 122
           CPEIVQYLGSEVTGGGDFLDD ELYNLKLEYAAGGTLADLIKQRNKLPEDEVKKYLQMIL
Sbjct: 62  CPEIVQYLGSEVTGGGDFLDDKELYNLKLEYAAGGTLADLIKQRNKLPEDEVKKYLQMIL 121

Query: 123 KGLSCIHRKGFVHVDLKPDNILAFPQSDGKMKLKIADFGLAERSCKRGEDDQEDRGSKYY 182
           KGLSCIHRKGFVHVDLKPDNILAFPQSDGKMKLKIADFGLAER  K GEDDQED+G ++Y
Sbjct: 122 KGLSCIHRKGFVHVDLKPDNILAFPQSDGKMKLKIADFGLAER-FKHGEDDQEDKGCEHY 181

Query: 183 SGALKVRATHRYMSPESIVFSEINGLHDIWSLGCTLVQMVSGERVWNDCKSYEELITKLL 242
            GAL+VRATHRYMSPESIV  EI+GLHDIWSLGC LVQMVSGERVWNDCK+YEEL+TKLL
Sbjct: 182 -GALRVRATHRYMSPESIVLKEIDGLHDIWSLGCVLVQMVSGERVWNDCKNYEELMTKLL 241

Query: 243 ISEEIPTIPEELSKQGKDFLENCFVRNYEQRWTADMLLQHPYLNEENKDTKNGDEKLKLP 302
            S+EIP IP+ELSKQG DFLE CFVR+Y+QRWTADMLLQHPYLNE+NKDT + DEKLKLP
Sbjct: 242 NSKEIPIIPKELSKQGIDFLEKCFVRDYKQRWTADMLLQHPYLNEQNKDTSD-DEKLKLP 301

Query: 303 KAIVFLPHQFFQSKTDMLLQQTKNGDEKLK-PSKAIFLPHQFFKSKA-QSCNN 354
           K   FLPHQFF  K DML    KNGDEKL+ P KAIFLPHQFF S A QSCN+
Sbjct: 302 KT-AFLPHQFFNRKADML----KNGDEKLELPKKAIFLPHQFFNSTAQQSCND 346

[Close]

BLAST of CSPI07G18190 vs. NCBI nr
Match: XP_008451652.1 (PREDICTED: serine/threonine-protein kinase Chk1-like [Cucumis melo])

HSP 1 Score: 466.5 bits (1199), Expect = 2.1e-127
Identity = 229/255 (89.80%), Postives = 241/255 (94.51%), Query Frame = 0


Query: 3   QDWVLVKALGEGSCGLVCLAKQITKEESDLHYYFAVKRASLRYNSSSLLREEHVLKHFTD 62
           +DWVL+K LGEGSCGLVCLAKQITKEESDLHYYFAVKRASLRYNS+SLL EEHVLKH TD
Sbjct: 2   EDWVLLKVLGEGSCGLVCLAKQITKEESDLHYYFAVKRASLRYNSASLLWEEHVLKHLTD 61

Query: 63  CPEIVQYLGSEVTGGGDFLDDMELYNLKLEYAAGGTLADLIKQRNKLPEDEVKKYLQMIL 122
           CPEIVQYLGSEVTGGGDFLDD ELYNLKLEYAAGGTLADLIKQRNKLPEDEVKKYLQMIL
Sbjct: 62  CPEIVQYLGSEVTGGGDFLDDKELYNLKLEYAAGGTLADLIKQRNKLPEDEVKKYLQMIL 121

Query: 123 KGLSCIHRKGFVHVDLKPDNILAFPQSDGKMKLKIADFGLAERSCKRGEDDQEDRGSKYY 182
           KGLSCIHRKGFVHVDLKPDNILAFPQSDGKMKLKIADFGLAER  K GEDDQED+G ++Y
Sbjct: 122 KGLSCIHRKGFVHVDLKPDNILAFPQSDGKMKLKIADFGLAER-FKHGEDDQEDKGCEHY 181

Query: 183 SGALKVRATHRYMSPESIVFSEINGLHDIWSLGCTLVQMVSGERVWNDCKSYEELITKLL 242
            GAL+VRATHRYMSPESIV  EI+GLHDIWSLGC LVQMVSGERVWNDCK+YEEL+TKLL
Sbjct: 182 -GALRVRATHRYMSPESIVLKEIDGLHDIWSLGCVLVQMVSGERVWNDCKNYEELMTKLL 241

Query: 243 ISEEIPTIPEELSKQ 258
            S+EIP IP+ELSKQ
Sbjct: 242 NSKEIPIIPKELSKQ 254

[Close]

BLAST of CSPI07G18190 vs. NCBI nr
Match: XP_038896325.1 (mitogen-activated protein kinase kinase kinase 17-like [Benincasa hispida])

HSP 1 Score: 455.3 bits (1170), Expect = 4.8e-124
Identity = 234/346 (67.63%), Postives = 272/346 (78.61%), Query Frame = 0


Query: 3   QDWVLVKALGEGSCGLVCLAKQITKEESDLHYYFAVKRASLRYNSSSLLREEHVLKHFTD 62
           ++WVLVK +G GS G+VCLAKQ+TKE+SD HYYFAVK  SL+   SSLL EE VLKHF D
Sbjct: 2   ENWVLVKIIGRGSYGVVCLAKQMTKEKSDRHYYFAVKLTSLQ-RKSSLLWEEQVLKHFKD 61

Query: 63  CPEIVQYLGSEVTGGGDFLDDMELYNLKLEYAAGGTLADLIKQRNKLPEDEVKKYLQMIL 122
           CPEIVQ LGSE+T  GD  DDM LYNLKLEYA GGTLADLIKQR KLPEDEVK YL+MIL
Sbjct: 62  CPEIVQCLGSEIT-FGDIPDDMVLYNLKLEYAPGGTLADLIKQRKKLPEDEVKDYLRMIL 121

Query: 123 KGLSCIHRKGFVHVDLKPDNILAFPQSDGKMKLKIADFGLAERSCKRGEDDQEDRGSKYY 182
           KGLSCIHR+GFVHVDLKP N+L FPQSDGKMKLKIADFGLAER CK   DDQ DRG    
Sbjct: 122 KGLSCIHRRGFVHVDLKPANVLVFPQSDGKMKLKIADFGLAER-CKHIVDDQ-DRGY--- 181

Query: 183 SGALKVRATHRYMSPESIVFSEINGLHDIWSLGCTLVQMVSGERVWNDCKSYEELITKLL 242
               K + T +YMSPESI F+E+NG HDIWSLGC LV+MVSG+ VW+DC+  E+L+T+LL
Sbjct: 182 --LRKFKGTPKYMSPESIFFNEVNGTHDIWSLGCILVKMVSGKSVWDDCRGSEQLMTRLL 241

Query: 243 ISEEIPTIPEELSKQGKDFLENCFVRNYEQRWTADMLLQHPYLNEENKDTKNGDEKLKLP 302
             + IPTIP+ELSKQGKDF++ CF+RNY+QRWTADMLLQHPYLNEEN+ TK GD+ +KL 
Sbjct: 242 NDKNIPTIPKELSKQGKDFIKRCFIRNYKQRWTADMLLQHPYLNEENEATKKGDDIVKLI 301

Query: 303 KAIVFLPHQFFQSKTDMLLQQTKNGDEKLKPSKAIFLPHQFFKSKA 349
           KAI  LPHQFF+ K         + +EK    K  +LPH  +K+K+
Sbjct: 302 KAI-RLPHQFFKRKA------KSSNNEKSNRQKEDYLPHLLYKNKS 331

[Close]

BLAST of CSPI07G18190 vs. NCBI nr
Match: XP_004137063.1 (mitogen-activated protein kinase kinase kinase 17 [Cucumis sativus] >KGN43756.1 hypothetical protein Csa_017187 [Cucumis sativus])

HSP 1 Score: 384.8 bits (987), Expect = 8.0e-103
Identity = 202/321 (62.93%), Postives = 247/321 (76.95%), Query Frame = 0


Query: 3   QDWVLVKALGEGSCGLVCLAKQITKEE--SDLHYYFAVKRASLRYNSSSLLREEHVLKHF 62
           ++WVLVK LG+GS GLVCLAKQ T+++  ++LHYYFAVK + L +NSS  L EE VLK F
Sbjct: 2   ENWVLVKILGQGSDGLVCLAKQTTRKKIYNNLHYYFAVKLSPLGHNSS--LWEELVLKQF 61

Query: 63  TDCPEIVQYLGSEVT---GGGDFLDDMELYNLKLEYAAGGTLADLIKQRNKLPEDEVKKY 122
            +CPEIVQYLGSE+T   GG D   D + Y LKLEYAAGGTL DLIKQ+ KLPEDEVK+Y
Sbjct: 62  KNCPEIVQYLGSEITRSPGGRDLPGDKDFYTLKLEYAAGGTLDDLIKQKEKLPEDEVKEY 121

Query: 123 LQMILKGLSCIHRKGFVHVDLKPDNILAFPQSDGKMKLKIADFGLAERSCKRGED----- 182
           L+MILKGLSCIH KGFVHVDLKP NILAFP+S+GKMKLKIADFG AER CK   D     
Sbjct: 122 LRMILKGLSCIHSKGFVHVDLKPKNILAFPRSNGKMKLKIADFGHAER-CKYRNDADAHS 181

Query: 183 DQEDRGSKYYSGALKVRATHRYMSPESIVFSEINGLHDIWSLGCTLVQMVSGERVWNDCK 242
           D E R S+  S  LK + T RYMSPESIVF E+N  HD+WSLGC LV+M+SG+  W++  
Sbjct: 182 DDEYRKSRRRSSLLKFKGTPRYMSPESIVFDEVNDAHDVWSLGCVLVRMISGKSAWDEVA 241

Query: 243 SYEELITKLLISEEIPTIPEELSKQGKDFLENCFVRNYEQRWTADMLLQHPYLNEENKDT 302
           + ++L+ K+L S+ +P IPEELS QGKDF++ CFV N++QRWTADMLLQHPYLNE+++ T
Sbjct: 242 TSKQLMMKILDSKSMPRIPEELSIQGKDFIKKCFVWNFKQRWTADMLLQHPYLNEKHEAT 301

Query: 303 --KNGDEKLKLPKAIVFLPHQ 312
             K+ D   KL K+++ L HQ
Sbjct: 302 TMKDDDGMPKLAKSLL-LSHQ 318

[Close]

BLAST of CSPI07G18190 vs. TAIR 10
Match: AT4G36950.1 (mitogen-activated protein kinase kinase kinase 21 )

HSP 1 Score: 194.1 bits (492), Expect = 1.9e-49
Identity = 115/299 (38.46%), Postives = 167/299 (55.85%), Query Frame = 0


Query: 4   DWVLVKALGEGSCGLVCLAKQITKEESDLHYYFAVKRASLRYNSSSLLREEHVLKHFTDC 63
           +W+  + +G GS   V LA              AVK + +   S++L  E  VL    DC
Sbjct: 2   EWIRRETIGHGSFSTVSLATTSGSSSKAFPSLMAVKSSGV-VCSAALRNERDVLDDLGDC 61

Query: 64  PEIVQYLGSEVTGGGDFLDDMELYNLKLEYAAGGTLADLIKQRNK-LPEDEVKKYLQMIL 123
            EIV+  G     G    +  E+YNL LEYA+GG+LAD IK   + LPE EV+++ + I+
Sbjct: 62  SEIVRCFGE----GRTVENGEEIYNLFLEYASGGSLADRIKSSGEALPEFEVRRFTRSIV 121

Query: 124 KGLSCIHRKGFVHVDLKPDNILAFPQSDGKMKLKIADFGLAERSCKRGEDDQEDRGSKYY 183
           KGL  IH  GF H D+K +N+L F   D    +KI+DFGLA+R  + GE           
Sbjct: 122 KGLCHIHGNGFTHCDIKLENVLVFGDGD----VKISDFGLAKR--RSGE----------- 181

Query: 184 SGALKVRATHRYMSPESIVFSEINGLHDIWSLGCTLVQMVSGERVW----NDCKSYEELI 243
              +++R T  YM+PES+   E     DIW+LGC++V+M SG+  W        +   L+
Sbjct: 182 -VCVEIRGTPLYMAPESVNHGEFESPADIWALGCSVVEMSSGKTAWCLEDGVMNNVMSLL 241

Query: 244 TKLLISEEIPTIPEELSKQGKDFLENCFVRNYEQRWTADMLLQHPYLNEENKDTKNGDE 298
            ++   +E+P IP ELS++GKDF+  CFV+N  +RWTA+MLL HP+L     D ++G+E
Sbjct: 242 VRIGSGDEVPRIPVELSEEGKDFVSKCFVKNAAERWTAEMLLDHPFL---AVDDESGEE 274

[Close]

BLAST of CSPI07G18190 vs. TAIR 10
Match: AT5G67080.1 (mitogen-activated protein kinase kinase kinase 19 )

HSP 1 Score: 185.3 bits (469), Expect = 8.7e-47
Identity = 107/287 (37.28%), Postives = 167/287 (58.19%), Query Frame = 0


Query: 4   DWVLVKALGEGSCGLVCLAKQITKEESDLHYYFAVKRASLRYNSSSLLREEHVLKHF-TD 63
           +W+  + +G G+   V LA +   +  +     AVK A   Y ++SL  E+ VL +   D
Sbjct: 2   EWIRGETIGYGTFSTVSLATRSNNDSGEFPPLMAVKSAD-SYGAASLANEKSVLDNLGDD 61

Query: 64  CPEIVQYLGSEVTGGGDFLDDMELYNLKLEYAAGGTLADLIKQ--RNKLPEDEVKKYLQM 123
           C EIV+  G + T      +  E++NL LEYA+ G+L   +K+     +PE  V+++   
Sbjct: 62  CNEIVRCFGEDRT----VENGEEMHNLFLEYASRGSLESYLKKLAGEGVPESTVRRHTGS 121

Query: 124 ILKGLSCIHRKGFVHVDLKPDNILAFPQSDGKMKLKIADFGLAERSCKRGEDDQEDRGSK 183
           +L+GL  IH  GF H DLK  NIL F    G   +KIADFGLA+R          D  + 
Sbjct: 122 VLRGLRHIHANGFAHCDLKLGNILLF----GDGAVKIADFGLAKRI--------GDLTAL 181

Query: 184 YYSGALKVRATHRYMSPESIVFSEINGLHDIWSLGCTLVQMVSGERVWN--DCKSYEELI 243
            Y   +++R T  YM+PES+  +E     D+W+LGC +V+M SG+  W+  +  ++  L+
Sbjct: 182 NY--GVQIRGTPLYMAPESVNDNEYGSEGDVWALGCVVVEMFSGKTAWSLKEGSNFMSLL 241

Query: 244 TKLLISEEIPTIPEELSKQGKDFLENCFVRNYEQRWTADMLLQHPYL 286
            ++ + +E+P IPEELS+QG+DFL  CFV++ ++RWTA+MLL HP++
Sbjct: 242 LRIGVGDEVPMIPEELSEQGRDFLSKCFVKDPKKRWTAEMLLNHPFV 269

[Close]

BLAST of CSPI07G18190 vs. TAIR 10
Match: AT3G50310.1 (mitogen-activated protein kinase kinase kinase 20 )

HSP 1 Score: 182.6 bits (462), Expect = 5.6e-46
Identity = 112/313 (35.78%), Postives = 173/313 (55.27%), Query Frame = 0


Query: 4   DWVLVKALGEGSCGLVCLAKQITKEESDLHYYFAVKRASLRYNSSSLLREEHVLKHFTDC 63
           +WV  + +G G+   V  A + ++   D     AVK     Y ++SL  E+ VL    DC
Sbjct: 2   EWVRGETIGFGTFSTVSTATK-SRNSGDFPALIAVKSTD-AYGAASLSNEKSVLDSLGDC 61

Query: 64  PEIVQYLGSEVTGGGDFLDDMELYNLKLEYAAGGTLADLIKQ--RNKLPEDEVKKYLQMI 123
           PEI++  G + T      +  E++NL LEYA+ G+LA  +K+     LPE  V+++   +
Sbjct: 62  PEIIRCYGEDST----VENGEEMHNLLLEYASRGSLASYMKKLGGEGLPESTVRRHTGSV 121

Query: 124 LKGLSCIHRKGFVHVDLKPDNILAFPQSDGKMKLKIADFGLAERSCKRGEDDQEDRGSKY 183
           L+GL  IH KGF H D+K  NIL F  +DG   +KIADFGLA R          D     
Sbjct: 122 LRGLRHIHAKGFAHCDIKLANILLF--NDG--SVKIADFGLAMR---------VDGDLTA 181

Query: 184 YSGALKVRATHRYMSPESIVFSEINGLHDIWSLGCTLVQMVSGERVWN--DCKSYEELIT 243
              ++++R T  YM+PE +  +E     D+W+LGC +V+M SG+  W+  +   +  L+ 
Sbjct: 182 LRKSVEIRGTPLYMAPECVNDNEYGSAADVWALGCAVVEMFSGKTAWSVKEGSHFMSLLI 241

Query: 244 KLLISEEIPTIPEELSKQGKDFLENCFVRNYEQRWTADMLLQHPYLNEENKD-------- 303
           ++ + +E+P IPE LS++GKDFL  CFV++  +RWTA+MLL H ++  + +D        
Sbjct: 242 RIGVGDELPKIPEMLSEEGKDFLSKCFVKDPAKRWTAEMLLNHSFVTIDLEDDHRENFVV 295

[Close]

BLAST of CSPI07G18190 vs. TAIR 10
Match: AT5G55090.1 (mitogen-activated protein kinase kinase kinase 15 )

HSP 1 Score: 174.9 bits (442), Expect = 1.2e-43
Identity = 109/313 (34.82%), Postives = 162/313 (51.76%), Query Frame = 0


Query: 2   DQDWVLVKALGEGSCGLVCLAKQITKEESDLHYYFAVKRASLRYNSSSLLREEHVLKHFT 61
           +Q+W+    +G GS   V L   IT        +FAVK  S  ++SS+ L+ E  +    
Sbjct: 3   EQNWIRGPIIGRGSTATVSLG--ITNSGD----FFAVK--SAEFSSSAFLQREQSILSKL 62

Query: 62  DCPEIVQYLGSEVTGGGDFLDDMELYNLKLEYAAGGTLADLIKQR-NKLPEDEVKKYLQM 121
             P IV+Y+GS VT   D L    +YNL +EY +GG+L DLIK    KLPE  ++ Y + 
Sbjct: 63  SSPYIVKYIGSNVTKENDKL----MYNLLMEYVSGGSLHDLIKNSGGKLPEPLIRSYTRQ 122

Query: 122 ILKGLSCIHRKGFVHVDLKPDNILAFPQSDGKMKLKIADFGLAERSCKRGEDDQEDRGSK 181
           ILKGL  +H +G VH D+K  N++      G    KI D G     C +  ++ E+    
Sbjct: 123 ILKGLMYLHDQGIVHCDVKSQNVMI-----GGEIAKIVDLG-----CAKTVEENEN---- 182

Query: 182 YYSGALKVRATHRYMSPESIVFSEINGLHDIWSLGCTLVQMVSGERVWNDCKSYEELITK 241
                L+   T  +MSPE     E +   D+W+LGCT+++M +G   W +       I K
Sbjct: 183 -----LEFSGTPAFMSPEVARGEEQSFPADVWALGCTVIEMATGSSPWPELNDVVAAIYK 242

Query: 242 LLISEEIPTIPEELSKQGKDFLENCFVRNYEQRWTADMLLQHPYLNEENKDTKNGDEKLK 301
           +  + E P IP  LS++G+DFL  C  ++ +QRWT + LLQHP+L+EE+ D+      L 
Sbjct: 243 IGFTGESPVIPVWLSEKGQDFLRKCLRKDPKQRWTVEELLQHPFLDEEDNDSDQTGNCLN 284

Query: 302 LPKAIVFLPHQFF 314
                  L  +F+
Sbjct: 303 SSSPSTVLDQRFW 284

[Close]

BLAST of CSPI07G18190 vs. TAIR 10
Match: AT4G26890.1 (mitogen-activated protein kinase kinase kinase 16 )

HSP 1 Score: 172.2 bits (435), Expect = 7.6e-43
Identity = 109/316 (34.49%), Postives = 165/316 (52.22%), Query Frame = 0


Query: 1   MDQDWVLVKALGEGSCGLVCLAKQITKEESDLHYYFAVKRASLRYNSSSLLREEHVLKHF 60
           M+ +W     +G GS   V +A   + E       FAVK A L  +SSSLL++E  +   
Sbjct: 1   MEINWTRGPIIGRGSTATVSIAISSSGE------LFAVKSADL--SSSSLLQKEQSILST 60

Query: 61  TDCPEIVQYLGSEVTGGGDFLDDMELYNLKLEYAAGGTLADLIKQR-NKLPEDEVKKYLQ 120
              P +V+Y+G+ +T   + L    +YN+ +EY +GG L DLIK    KLPE E++ Y +
Sbjct: 61  LSSPHMVKYIGTGLTRESNGL----VYNILMEYVSGGNLHDLIKNSGGKLPEPEIRSYTR 120

Query: 121 MILKGLSCIHRKGFVHVDLKPDNILAFPQSDGKMKLKIADFGLAERSCKRGEDDQEDRGS 180
            IL GL  +H +G VH DLK  N+L   + +G   LKIAD G     C +  D  E    
Sbjct: 121 QILNGLVYLHERGIVHCDLKSHNVLV--EENG--VLKIADMG-----CAKSVDKSE---- 180

Query: 181 KYYSGALKVRATHRYMSPESIVFSEINGLHDIWSLGCTLVQMVSGERVWNDCKSYEELIT 240
             +SG      T  +M+PE     E     D+W+LGCT+++M++G   W +       + 
Sbjct: 181 --FSG------TPAFMAPEVARGEEQRFPADVWALGCTMIEMMTGSSPWPELNDVVAAMY 240

Query: 241 KLLISEEIPTIPEELSKQGKDFLENCFVRNYEQRWTADMLLQHPYLNEENKDTKNGDEKL 300
           K+  S E P IP  +S + KDFL+NC   + +QRWT + LL+HP+L+++ +   +   K 
Sbjct: 241 KIGFSGESPAIPAWISDKAKDFLKNCLKEDQKQRWTVEELLKHPFLDDDEESQTSDCLKN 283

Query: 301 KLPKAIVFLPHQFFQS 316
           K       L  +F+ S
Sbjct: 301 KTSSPSTVLDQRFWDS 283

The following BLAST results are available for this feature:

BLAST of CSPI07G18190 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SND6	7.9e-45	35.78	Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=370...	[more]
O80888	1.4e-33	34.62	Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=370...	[more]
Q01389	8.5e-31	32.65	Serine/threonine-protein kinase BCK1/SLK1/SSP31 OS=Saccharomyces cerevisiae (str...	[more]
Q40541	8.5e-31	29.24	Mitogen-activated protein kinase kinase kinase NPK1 OS=Nicotiana tabacum OX=4097...	[more]
Q9CAD5	1.5e-30	33.22	Mitogen-activated protein kinase kinase kinase YODA OS=Arabidopsis thaliana OX=3...	[more]

BLAST of CSPI07G18190 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K835	2.3e-204	99.15	Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G407...	[more]
A0A5A7TZP4	7.7e-168	85.55	Serine/threonine-protein kinase Chk1-like OS=Cucumis melo var. makuwa OX=1194695...	[more]
A0A1S3BRE6	1.0e-127	89.80	serine/threonine-protein kinase Chk1-like OS=Cucumis melo OX=3656 GN=LOC10349288...	[more]
A0A0A0K7G3	3.9e-103	62.93	Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G065...	[more]
A0A5D3C4R6	1.9e-102	58.69	Mitogen-activated protein kinase kinase kinase A-like OS=Cucumis melo var. makuw...	[more]

BLAST of CSPI07G18190 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004136151.1	4.7e-204	99.15	mitogen-activated protein kinase kinase kinase 17 [Cucumis sativus] >KGN45019.1 ...	[more]
KAA0046699.1	1.6e-167	85.55	serine/threonine-protein kinase Chk1-like [Cucumis melo var. makuwa] >TYK18235.1...	[more]
XP_008451652.1	2.1e-127	89.80	PREDICTED: serine/threonine-protein kinase Chk1-like [Cucumis melo]	[more]
XP_038896325.1	4.8e-124	67.63	mitogen-activated protein kinase kinase kinase 17-like [Benincasa hispida]	[more]
XP_004137063.1	8.0e-103	62.93	mitogen-activated protein kinase kinase kinase 17 [Cucumis sativus] >KGN43756.1 ...	[more]

BLAST of CSPI07G18190 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G36950.1	1.9e-49	38.46	mitogen-activated protein kinase kinase kinase 21	[more]
AT5G67080.1	8.7e-47	37.28	mitogen-activated protein kinase kinase kinase 19	[more]
AT3G50310.1	5.6e-46	35.78	mitogen-activated protein kinase kinase kinase 20	[more]
AT5G55090.1	1.2e-43	34.82	mitogen-activated protein kinase kinase kinase 15	[more]
AT4G26890.1	7.6e-43	34.49	mitogen-activated protein kinase kinase kinase 16	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000719	Protein kinase domain	SMART	SM00220	serkin_6	coord: 5..285 e-value: 1.2E-56 score: 204.1
IPR000719	Protein kinase domain	PFAM	PF00069	Pkinase	coord: 7..285 e-value: 2.7E-49 score: 167.9
IPR000719	Protein kinase domain	PROSITE	PS50011	PROTEIN_KINASE_DOM	coord: 5..285 score: 38.49184
None	No IPR available	GENE3D	1.10.510.10	Transferase(Phosphotransferase) domain 1	coord: 1..349 e-value: 1.5E-58 score: 200.5
None	No IPR available	PIRSR	PIRSR038172-1	PIRSR038172-1	coord: 3..287 e-value: 1.6E-11 score: 41.1
None	No IPR available	PIRSR	PIRSR000620-1	PIRSR000620-1	coord: 6..277 e-value: 2.8E-15 score: 53.9
None	No IPR available	PANTHER	PTHR48011	CCR4-NOT TRANSCRIPTIONAL COMPLEX SUBUNIT CAF120-RELATED	coord: 4..306
None	No IPR available	PANTHER	PTHR48011:SF51	PROTEIN KINASE SUPERFAMILY PROTEIN	coord: 4..306
IPR011009	Protein kinase-like domain superfamily	SUPERFAMILY	56112	Protein kinase-like (PK-like)	coord: 3..291

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI07G18190.1	CSPI07G18190.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006468	protein phosphorylation
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0016798	hydrolase activity, acting on glycosyl bonds
molecular_function	GO:0004672	protein kinase activity