CSPI07G16060 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI07G16060
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionKinase family protein
LocationChr7: 14696488 .. 14700876 (-)
RNA-Seq ExpressionCSPI07G16060
SyntenyCSPI07G16060
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTTTTTCCCCCCTTTTCCCATTTTGTCGATTTTGTTATTGTTCTTCCTCAATCTACTTTTATTTTTATTTTTATTTTTCCCTTTTTCCGATTCTTTACTCTCCATTTTTTTGCTCTGTGAATTGTTGTCCACAAAAGCTGCCTCTCCCTTTCGCTTTCTTTCACCAATTCAACAATACCCATTTCTCTTTTCTTCTCTATTTCCCTTCTTTGGCTTCTTCATTTCAACCCCTCTTCCATATCTGTGAAGGGGTATTTGCCTCCTTCATTTCTGTTATTCTTTCTTTCTTTTTTGTTGTTTTTTCTTACGATTCTGCATATGGGTTGATTTTCTCTAGCCTTCTTTATCGCCAAATTCGCAGAATTTGGTCGAAGATACTCAAATTTCACAGTTCTTAGCTCCTAGATTTGTGGGGGTGGCTGCTGGCTGGAACAGTATTTGCAAGTCCAGTTTTTTTATTTTTCAGTACCATAATATTTTATTAAACGTTTGAGTTCCTTGGTTAACCTTTTTTTATATAAAAGAAACAAAAGAAGGGCTGAGAGATTATTGGTTGTTTGTTTGTTTTTTTTTTTTGTTTTTGTTTTAGTTGATTTCATGGTGGTTTCGTTGAACTGGTAACTTACTGATTTTCTTCTTCTTGTCATCTTCATCAAGGTTTTTTGACCAATTGCTTGGTTTGGTTTTTATTGTCTTCCATGCCTCTGTAAAAGTTCCCCAGTGTTTTCAAAATTTGCTTTGTTATCTTTTATACTCTCTACTTTCAATCTATCTATCTATCTATCTATCAATAAAGCTTTATTAAAACATGGATTTAGCGAATGGGGGCGAGGCCGGCAAGGCTCTGGTGGCTAATCAAGTAGCTGATATTGTTTCTGGTAAATCTAGAAATACCCAAGAGAATGATTTGGGTTCAAAGTTGGGGACAGGAAGCAAAAGCAATAGGGATATGGTGTTTCGTGCTGATAAGATTGATTTCAAGAGTTGGGATATTCAGCTGGAGAAGCACTTGAGCAGGGCTTGGTCAAGGGACAGGGAAGTGCCTGCTAAAAAGGAAGAATGGGAGATTGACTTGTCTAAACTTGATATCAGATATGTTAAAGCTCATGGAACTTACGGTACTATTTACAGAGGAAACTATGACGGCAACGATGTTGCAGGTAATTTCGACCTATTTTTAGAGTATTTTTTCACCATGTCGACAATCGTCGTGATAATTGACCATTTGTTCTTGATTAGATATGGAGCAGTTTTCAGAGGAAACCACGTGTTCTTGATTAGATATGGATTTTTTTCCTTTATCCTTCTTGTTTGCATGTACTAAATAGCCAAATCATCTGCGAAAGTATTATGATATCCTGATATATAACTTTTCGAACCTGACATAGCTAATTATGTTTAATCTGATGGGGTCTACTTATGTCTAACTTGAGTGCAAAACAACGTTGAATGTGGTTCCCATTTAGGTTGTCTGTCCTACAAGAATACTGCTCTGAAAGTTATAGAATTGATAACATTTTGGAAGTACTTTCAGCTCCACACATAAACGTGTCCTGCTTGTTAGCTTATTATTATTGGACAATTTCGTCAAGTTTTGGTTTGTTTTATCGATTTATGACTGATATTCTGCATACTGCAGTGAAAGTGCTTGATTGGGGGGAGGATGGTGTTTCCTCAGTCGCTGAAATTGCTGCTCTTCGAACATCTTTTCGCCAGGAAGTTGCGGTATGGCATAAGCTTGACCATCCCAATGTTGCAAAGGTACAACTTCAAGTATCTTTTACACGTAGAATACGGTATCACACTATGTCAAACAAATCAAACAAATCAAACCATTCATTGTTAGTCTAATGATGTTCTTTACATTTTCATCCAACATCATAGGCATGCTTAGAGATTATGCTAACAGGGAAGGCTTTGATTCTTTACATTTGATGCTTGCACTATCCTAAATGTTCAGATTCTCAAGATATAAATTTCGTTTTACATGATTGGTTCTCTTGCCTAACTTTTAGAATAAGAATCCATTCAAGTTGCTCCTGCTGAAATGTCTCACTCTCAAGTCTCAACTAATCTTTGTTGTCTGTTCTGGGGTTTCTGTGTTATAAACTTTGTTTCTTCACTGTGTTTGATATGTGAAGATCAAATAATTATATGAAATTTCTGTTAGTTTTATGGAGCATCAATGGGGACGTCAAACCTTAAAATCCCTCCCAAAAGCTCATCATTCGACAGTAACCAAACTTTTCCTTCAAGGGCTTGCTGCGTTGTTGTTGAGTATCTTCCAGGTGGAACCCTAAAAAGCTTTTTAATCAAAAATAGGAAAAGGAAACTTGCATTTAAGATTGTGATTCAACTTGCATTGGATCTTTCTAGAGGGTAAGTTAAAGTTATCTTCGACAAATCACTCCAATATGCCAATGTTTTGGATTTCACGTTTTGAGGCAATTGCTTATTTCGATTTTGCAGTTTGAGTTATCTACACTCCAAAAAGATTGTACACCGTGATATTAAAACAGAGAATGTTCTGCTAGATGCTCAGAAGACTCTGAAAATTGTTGACTTTGGCGTTGCTCGAGTCGAAGCACAGAACCCAAAGGATATGACTGGAGAGACTGGCACCCTTGGTTACATGGCCCCCGAGGTTTCCTTTTTCTCTAAAATCAAGTTCTAGAATGTACTATTAAGAGATTATGATTGATGTCTTGATGAATGGTCGTCGTTAACAGATCCAATTCTCATTTGGGTTGCAATTGTTTCCTCTTGTTGTGTGAATAAAGGAATCTAACTAACATTTTTTTCAACAGGTCCTTGATGGTAAGCCTTATAATCGGAAATGTGATGTTTACAGTCTTGGTATATGTTTGTGGGAAACATATTGCTGCGATATGCCTTACCCAGATCTCAGTTTTGCTGAAGTGTCTACTGCAGTTGTGCGGCAGGTATGTTTCTTTTATAAACTTCGCAATTATTTTAGATCTCATTTGATAACCATTTGGTTTTTTGAAATAAAGTCTATTTTTTTATTAATTTCTTATCGAAGTTTGCATCTTTCTTAAGTAAAAGAGTTGAATTATTTGTCAAATTCCAAAATTAAAAAATAAATCTAAAAACTACTTTGTTTAGTTTTCAAATTTTGGGTTTGTGTTTGAAAACATTGATAAAAAGTAGTTAACAAAGCATGAAATTTAAAGGTGAAAGAAGTGTTTGTAGGCTTAATTTTCAAAAAATAAAAACCCAAAATCAAATGGTTACCTTAGTTTTCTTCAAAGCTTGTGACTTCTTATTGTTTATACTAATTATACAAAATGGTGTAATTCAACTTCCAAAATAAATTCTTAATATATCCTCATTTTGAGTATTATTTGGTTGATCTTTTGGAGTGGGGAGGAGGTGTAGCTCGGCCAGTCTGAATTGGCCATTGACTAAAATTGATGGGCTAATCAATAGTTTTTCGTCATAATTGGCAACTAGTCTCATGACCTATTTAGAGAAGAATGTCCTTTTAAGACTTGGATTGGACATTTGATGATCATCGAAATAGACAGAAACATTAGTTTTTGCTCTTACTAGTTTCCCATCTATGGACCTTTTCTTTTGCTTCATGTATTCAAAGATTTTATTAAATAGAAAATTTCTTAGTGGTTTGCTCAAATTATCTGTTTCCATGATAGGAGGAACTTTAGCTCAAATGTGAAATGGGAGAAAATTCCTTGGTTACGTTCATCTTTTCCCAATGTTTATAAAAAACATTAAACTAGCCTTGTTTGAAATCCATGTTGACAAAATTTGTTAAGATGATGAACAGTGTTGTATTCAAGATAGGGTATTTGGAGTGTGTTCTGAAGTTGAATTCATGCTTAAAAATTACTTGCAACAGAATTTACGACCAGATATCCCTAGGTGCTGCCCAAGTTCGTTTGCAAATATCATGAAAAGATGTTGGGACGCAAATCCAGAAAAGCGACCCGACATGGATGAGGTCGTAAAACTGTTGGAAGCCATTGATACAAGTAAAGGGGGTGGCATGATTACTGAAGATCAGATTAGCTGTTTCTGTTTCCGCCCGGCTCGTGGTCCATAAATTTCGACATCTTTGCTACACCATTTACTTGCAGTTGAAGATGGTTTAGAGAAGTCATTGGGATGATCCATCATAATACTCATTGGTTCATACAATACATTTCTCAAGGTCATGGCTTTGGGTTTCATAGTATGAACTTTTGATGCCAAATAGAAATTTAGAATAATCATATGTCAAACCATTCGTTGGTGTAATTATGTTTTATTGCATAGTTTTGCATTCTTATGAATGTTTAGTTACAAGGAACAGCTGATTGGCACACACACATATATATATATATTTTTGGGCTCAAACTGATTTGGCA

mRNA sequence

CTTTTTTCCCCCCTTTTCCCATTTTGTCGATTTTGTTATTGTTCTTCCTCAATCTACTTTTATTTTTATTTTTATTTTTCCCTTTTTCCGATTCTTTACTCTCCATTTTTTTGCTCTGTGAATTGTTGTCCACAAAAGCTGCCTCTCCCTTTCGCTTTCTTTCACCAATTCAACAATACCCATTTCTCTTTTCTTCTCTATTTCCCTTCTTTGGCTTCTTCATTTCAACCCCTCTTCCATATCTGTGAAGGGGTATTTGCCTCCTTCATTTCTGTTATTCTTTCTTTCTTTTTTGTTGTTTTTTCTTACGATTCTGCATATGGGTTGATTTTCTCTAGCCTTCTTTATCGCCAAATTCGCAGAATTTGGTCGAAGATACTCAAATTTCACAGTTCTTAGCTCCTAGATTTGTGGGGGTGGCTGCTGGCTGGAACAGTATTTGCAAGTCCAGTTTTTTTATTTTTCAGTACCATAATATTTTATTAAACGTTTGAGTTCCTTGGTTAACCTTTTTTTATATAAAAGAAACAAAAGAAGGGCTGAGAGATTATTGGTTGTTTGTTTGTTTTTTTTTTTTGTTTTTGTTTTAGTTGATTTCATGGTGGTTTCGTTGAACTGGTAACTTACTGATTTTCTTCTTCTTGTCATCTTCATCAAGGTTTTTTGACCAATTGCTTGGTTTGGTTTTTATTGTCTTCCATGCCTCTGTAAAAGTTCCCCAGTGTTTTCAAAATTTGCTTTGTTATCTTTTATACTCTCTACTTTCAATCTATCTATCTATCTATCTATCAATAAAGCTTTATTAAAACATGGATTTAGCGAATGGGGGCGAGGCCGGCAAGGCTCTGGTGGCTAATCAAGTAGCTGATATTGTTTCTGGTAAATCTAGAAATACCCAAGAGAATGATTTGGGTTCAAAGTTGGGGACAGGAAGCAAAAGCAATAGGGATATGGTGTTTCGTGCTGATAAGATTGATTTCAAGAGTTGGGATATTCAGCTGGAGAAGCACTTGAGCAGGGCTTGGTCAAGGGACAGGGAAGTGCCTGCTAAAAAGGAAGAATGGGAGATTGACTTGTCTAAACTTGATATCAGATATGTTAAAGCTCATGGAACTTACGGTACTATTTACAGAGGAAACTATGACGGCAACGATGTTGCAGTGAAAGTGCTTGATTGGGGGGAGGATGGTGTTTCCTCAGTCGCTGAAATTGCTGCTCTTCGAACATCTTTTCGCCAGGAAGTTGCGGTATGGCATAAGCTTGACCATCCCAATGTTGCAAAGTTTTATGGAGCATCAATGGGGACGTCAAACCTTAAAATCCCTCCCAAAAGCTCATCATTCGACAGTAACCAAACTTTTCCTTCAAGGGCTTGCTGCGTTGTTGTTGAGTATCTTCCAGGTGGAACCCTAAAAAGCTTTTTAATCAAAAATAGGAAAAGGAAACTTGCATTTAAGATTGTGATTCAACTTGCATTGGATCTTTCTAGAGGTTTGAGTTATCTACACTCCAAAAAGATTGTACACCGTGATATTAAAACAGAGAATGTTCTGCTAGATGCTCAGAAGACTCTGAAAATTGTTGACTTTGGCGTTGCTCGAGTCGAAGCACAGAACCCAAAGGATATGACTGGAGAGACTGGCACCCTTGGTTACATGGCCCCCGAGGTCCTTGATGGTAAGCCTTATAATCGGAAATGTGATGTTTACAGTCTTGGTATATGTTTGTGGGAAACATATTGCTGCGATATGCCTTACCCAGATCTCAGTTTTGCTGAAGTGTCTACTGCAGTTGTGCGGCAGAATTTACGACCAGATATCCCTAGGTGCTGCCCAAGTTCGTTTGCAAATATCATGAAAAGATGTTGGGACGCAAATCCAGAAAAGCGACCCGACATGGATGAGGTCGTAAAACTGTTGGAAGCCATTGATACAAGTAAAGGGGGTGGCATGATTACTGAAGATCAGATTAGCTGTTTCTGTTTCCGCCCGGCTCGTGGTCCATAAATTTCGACATCTTTGCTACACCATTTACTTGCAGTTGAAGATGGTTTAGAGAAGTCATTGGGATGATCCATCATAATACTCATTGGTTCATACAATACATTTCTCAAGGTCATGGCTTTGGGTTTCATAGTATGAACTTTTGATGCCAAATAGAAATTTAGAATAATCATATGTCAAACCATTCGTTGGTGTAATTATGTTTTATTGCATAGTTTTGCATTCTTATGAATGTTTAGTTACAAGGAACAGCTGATTGGCACACACACATATATATATATATTTTTGGGCTCAAACTGATTTGGCA

Coding sequence (CDS)

ATGGATTTAGCGAATGGGGGCGAGGCCGGCAAGGCTCTGGTGGCTAATCAAGTAGCTGATATTGTTTCTGGTAAATCTAGAAATACCCAAGAGAATGATTTGGGTTCAAAGTTGGGGACAGGAAGCAAAAGCAATAGGGATATGGTGTTTCGTGCTGATAAGATTGATTTCAAGAGTTGGGATATTCAGCTGGAGAAGCACTTGAGCAGGGCTTGGTCAAGGGACAGGGAAGTGCCTGCTAAAAAGGAAGAATGGGAGATTGACTTGTCTAAACTTGATATCAGATATGTTAAAGCTCATGGAACTTACGGTACTATTTACAGAGGAAACTATGACGGCAACGATGTTGCAGTGAAAGTGCTTGATTGGGGGGAGGATGGTGTTTCCTCAGTCGCTGAAATTGCTGCTCTTCGAACATCTTTTCGCCAGGAAGTTGCGGTATGGCATAAGCTTGACCATCCCAATGTTGCAAAGTTTTATGGAGCATCAATGGGGACGTCAAACCTTAAAATCCCTCCCAAAAGCTCATCATTCGACAGTAACCAAACTTTTCCTTCAAGGGCTTGCTGCGTTGTTGTTGAGTATCTTCCAGGTGGAACCCTAAAAAGCTTTTTAATCAAAAATAGGAAAAGGAAACTTGCATTTAAGATTGTGATTCAACTTGCATTGGATCTTTCTAGAGGTTTGAGTTATCTACACTCCAAAAAGATTGTACACCGTGATATTAAAACAGAGAATGTTCTGCTAGATGCTCAGAAGACTCTGAAAATTGTTGACTTTGGCGTTGCTCGAGTCGAAGCACAGAACCCAAAGGATATGACTGGAGAGACTGGCACCCTTGGTTACATGGCCCCCGAGGTCCTTGATGGTAAGCCTTATAATCGGAAATGTGATGTTTACAGTCTTGGTATATGTTTGTGGGAAACATATTGCTGCGATATGCCTTACCCAGATCTCAGTTTTGCTGAAGTGTCTACTGCAGTTGTGCGGCAGAATTTACGACCAGATATCCCTAGGTGCTGCCCAAGTTCGTTTGCAAATATCATGAAAAGATGTTGGGACGCAAATCCAGAAAAGCGACCCGACATGGATGAGGTCGTAAAACTGTTGGAAGCCATTGATACAAGTAAAGGGGGTGGCATGATTACTGAAGATCAGATTAGCTGTTTCTGTTTCCGCCCGGCTCGTGGTCCATAA

Protein sequence

MDLANGGEAGKALVANQVADIVSGKSRNTQENDLGSKLGTGSKSNRDMVFRADKIDFKSWDIQLEKHLSRAWSRDREVPAKKEEWEIDLSKLDIRYVKAHGTYGTIYRGNYDGNDVAVKVLDWGEDGVSSVAEIAALRTSFRQEVAVWHKLDHPNVAKFYGASMGTSNLKIPPKSSSFDSNQTFPSRACCVVVEYLPGGTLKSFLIKNRKRKLAFKIVIQLALDLSRGLSYLHSKKIVHRDIKTENVLLDAQKTLKIVDFGVARVEAQNPKDMTGETGTLGYMAPEVLDGKPYNRKCDVYSLGICLWETYCCDMPYPDLSFAEVSTAVVRQNLRPDIPRCCPSSFANIMKRCWDANPEKRPDMDEVVKLLEAIDTSKGGGMITEDQISCFCFRPARGP*
Homology
BLAST of CSPI07G16060 vs. ExPASy Swiss-Prot
Match: Q9ZQ31 (Serine/threonine-protein kinase STY13 OS=Arabidopsis thaliana OX=3702 GN=STY13 PE=1 SV=2)

HSP 1 Score: 242.3 bits (617), Expect = 9.5e-63
Identity = 118/293 (40.27%), Postives = 180/293 (61.43%), Query Frame = 0

Query: 83  EEWEIDLSKLDIRYVKAHGTYGTIYRGNYDGNDVAVKVLDWGEDGVSSVAEIAALRTSFR 142
           +EW IDL KL++    A G +G +Y+G Y+G DVA+K+L+  E   +S  +   +   F+
Sbjct: 121 DEWTIDLRKLNMGPAFAQGAFGKLYKGTYNGEDVAIKILERPE---NSPEKAQFMEQQFQ 180

Query: 143 QEVAVWHKLDHPNVAKFYGASMGTSNLKIPPKSSSFDSNQTFPSRACCVVVEYLPGGTLK 202
           QEV++   L HPN+ +F GA                           C+V EY  GG+++
Sbjct: 181 QEVSMLANLKHPNIVRFIGACR--------------------KPMVWCIVTEYAKGGSVR 240

Query: 203 SFLIKNRKRKLAFKIVIQLALDLSRGLSYLHSKKIVHRDIKTENVLLDAQKTLKIVDFGV 262
            FL + + R +  K+ ++ ALD++RG++Y+H +  +HRD+K++N+L+ A K++KI DFGV
Sbjct: 241 QFLTRRQNRAVPLKLAVKQALDVARGMAYVHGRNFIHRDLKSDNLLISADKSIKIADFGV 300

Query: 263 ARVEAQNPKDMTGETGTLGYMAPEVLDGKPYNRKCDVYSLGICLWETYCCDMPYPDLSFA 322
           AR+E Q  + MT ETGT  +MAPE++  + YN+K DVYS GI LWE     +P+ +++  
Sbjct: 301 ARIEVQT-EGMTPETGTYRWMAPEMIQHRAYNQKVDVYSFGIVLWELITGLLPFQNMTAV 360

Query: 323 EVSTAVVRQNLRPDIPRCCPSSFANIMKRCWDANPEKRPDMDEVVKLLEAIDT 376
           + + AVV + +RP +P  C    ++IM RCWDANPE RP   EVVKLLEA +T
Sbjct: 361 QAAFAVVNRGVRPTVPNDCLPVLSDIMTRCWDANPEVRPCFVEVVKLLEAAET 389

BLAST of CSPI07G16060 vs. ExPASy Swiss-Prot
Match: Q2MHE4 (Serine/threonine/tyrosine-protein kinase HT1 OS=Arabidopsis thaliana OX=3702 GN=HT1 PE=1 SV=1)

HSP 1 Score: 216.1 bits (549), Expect = 7.3e-55
Identity = 116/317 (36.59%), Postives = 172/317 (54.26%), Query Frame = 0

Query: 58  KSWDIQLEKHLSRAWSRDREVPAKKEEWEIDLSKLDIRYVKAHGTYGTIYRGNYDGNDVA 117
           +SW + LE      W   +    ++EEW  DLS+L I    A G +  IYRG Y    VA
Sbjct: 55  ESWSMILESENVETWEASK---GEREEWTADLSQLFIGNKFASGAHSRIYRGIYKQRAVA 114

Query: 118 VKVLDWGEDGVSSVAEIAALRTSFRQEVAVWHKLDHPNVAKFYGASMGTSNLKIPPKSSS 177
           VK++             A L   F+ EVA+  +L HPN+ +F  A       K PP    
Sbjct: 115 VKMV---RIPTHKEETRAKLEQQFKSEVALLSRLFHPNIVQFIAA------CKKPP---- 174

Query: 178 FDSNQTFPSRACCVVVEYLPGGTLKSFLIKNRKRKLAFKIVIQLALDLSRGLSYLHSKKI 237
                       C++ EY+  G L+ +L K     L+ + V++LALD+SRG+ YLHS+ +
Sbjct: 175 ----------VYCIITEYMSQGNLRMYLNKKEPYSLSIETVLRLALDISRGMEYLHSQGV 234

Query: 238 VHRDIKTENVLLDAQKTLKIVDFGVARVEAQNPKDMTGETGTLGYMAPEVLDGKPYNRKC 297
           +HRD+K+ N+LL+ +  +K+ DFG + +E Q  ++  G  GT  +MAPE++  KPY RK 
Sbjct: 235 IHRDLKSNNLLLNDEMRVKVADFGTSCLETQ-CREAKGNMGTYRWMAPEMIKEKPYTRKV 294

Query: 298 DVYSLGICLWETYCCDMPYPDLSFAEVSTAVVRQNLRPDIPRCCPSSFANIMKRCWDANP 357
           DVYS GI LWE     +P+  ++  + + AV  +N RP +P  C  + A+++KRCW  NP
Sbjct: 295 DVYSFGIVLWELTTALLPFQGMTPVQAAFAVAEKNERPPLPASCQPALAHLIKRCWSENP 344

Query: 358 EKRPDMDEVVKLLEAID 375
            KRPD   +V +LE  D
Sbjct: 355 SKRPDFSNIVAVLEKYD 344

BLAST of CSPI07G16060 vs. ExPASy Swiss-Prot
Match: O22558 (Serine/threonine-protein kinase STY8 OS=Arabidopsis thaliana OX=3702 GN=STY8 PE=1 SV=2)

HSP 1 Score: 211.8 bits (538), Expect = 1.4e-53
Identity = 114/300 (38.00%), Postives = 177/300 (59.00%), Query Frame = 0

Query: 77  EVPAK-KEEWEIDLSKLDIRYVKAHGTYGTIYRGNYDGNDVAVKVLDWGEDGVSSVAEIA 136
           E+P    +EWEID+++L I    A G+YG ++RG Y   +VA+K L    D V++     
Sbjct: 270 EIPTDGTDEWEIDVTQLKIEKKVASGSYGDLHRGTYCSQEVAIKFLK--PDRVNN----- 329

Query: 137 ALRTSFRQEVAVWHKLDHPNVAKFYGASMGTSNLKIPPKSSSFDSNQTFPSRACCVVVEY 196
            +   F QEV +  K+ H NV +F GA   +  L                    C+V E+
Sbjct: 330 EMLREFSQEVFIMRKVRHKNVVQFLGACTRSPTL--------------------CIVTEF 389

Query: 197 LPGGTLKSFLIKNRKRKLAFKI--VIQLALDLSRGLSYLHSKKIVHRDIKTENVLLDAQK 256
           +  G++  FL    K+K AFK+  ++++ALD+++G+SYLH   I+HRD+KT N+L+D   
Sbjct: 390 MARGSIYDFL---HKQKCAFKLQTLLKVALDVAKGMSYLHQNNIIHRDLKTANLLMDEHG 449

Query: 257 TLKIVDFGVARVEAQNPKDMTGETGTLGYMAPEVLDGKPYNRKCDVYSLGICLWETYCCD 316
            +K+ DFGVARV+ ++   MT ETGT  +MAPEV++ KPYN K DV+S  I LWE    D
Sbjct: 450 LVKVADFGVARVQIESGV-MTAETGTYRWMAPEVIEHKPYNHKADVFSYAIVLWELLTGD 509

Query: 317 MPYPDLSFAEVSTAVVRQNLRPDIPRCCPSSFANIMKRCWDANPEKRPDMDEVVKLLEAI 374
           +PY  L+  + +  VV++ LRP IP+        +++RCW  +PE+RP  +E++++L+ I
Sbjct: 510 IPYAFLTPLQAAVGVVQKGLRPKIPKKTHPKVKGLLERCWHQDPEQRPLFEEIIEMLQQI 538

BLAST of CSPI07G16060 vs. ExPASy Swiss-Prot
Match: Q8RWL6 (Serine/threonine-protein kinase STY17 OS=Arabidopsis thaliana OX=3702 GN=STY17 PE=1 SV=1)

HSP 1 Score: 205.3 bits (521), Expect = 1.3e-51
Identity = 109/300 (36.33%), Postives = 174/300 (58.00%), Query Frame = 0

Query: 77  EVPAK-KEEWEIDLSKLDIRYVKAHGTYGTIYRGNYDGNDVAVKVLDWGEDGVSSVAEIA 136
           E+P    +EWEID+ +L I    A G+YG ++RG Y   +VA+K+L    + V+     A
Sbjct: 276 EIPTDGTDEWEIDMKQLKIEKKVACGSYGELFRGTYCSQEVAIKILK--PERVN-----A 335

Query: 137 ALRTSFRQEVAVWHKLDHPNVAKFYGASMGTSNLKIPPKSSSFDSNQTFPSRACCVVVEY 196
            +   F QEV +  K+ H NV +F GA   + NL                    C+V E+
Sbjct: 336 EMLREFSQEVYIMRKVRHKNVVQFIGACTRSPNL--------------------CIVTEF 395

Query: 197 LPGGTLKSFLIKNRKRKLAFKI--VIQLALDLSRGLSYLHSKKIVHRDIKTENVLLDAQK 256
           +  G++  FL    K K  FKI  ++++ALD+S+G++YLH   I+HRD+KT N+L+D  +
Sbjct: 396 MTRGSIYDFL---HKHKGVFKIQSLLKVALDVSKGMNYLHQNNIIHRDLKTANLLMDEHE 455

Query: 257 TLKIVDFGVARVEAQNPKDMTGETGTLGYMAPEVLDGKPYNRKCDVYSLGICLWETYCCD 316
            +K+ DFGVARV+ ++   MT ETGT  +MAPEV++ KPY+ + DV+S  I LWE    +
Sbjct: 456 VVKVADFGVARVQTESGV-MTAETGTYRWMAPEVIEHKPYDHRADVFSYAIVLWELLTGE 515

Query: 317 MPYPDLSFAEVSTAVVRQNLRPDIPRCCPSSFANIMKRCWDANPEKRPDMDEVVKLLEAI 374
           +PY  L+  + +  VV++ LRP IP+        ++++CW  +P  RP+  E++++L  +
Sbjct: 516 LPYSYLTPLQAAVGVVQKGLRPKIPKETHPKLTELLEKCWQQDPALRPNFAEIIEMLNQL 544

BLAST of CSPI07G16060 vs. ExPASy Swiss-Prot
Match: F4JTP5 (Serine/threonine-protein kinase STY46 OS=Arabidopsis thaliana OX=3702 GN=STY46 PE=1 SV=1)

HSP 1 Score: 202.6 bits (514), Expect = 8.3e-51
Identity = 109/296 (36.82%), Postives = 168/296 (56.76%), Query Frame = 0

Query: 85  WEIDLSKLDIRYVKAHGTYGTIYRGNYDGNDVAVKVLDWGEDGVSSVAEIAALRTSFRQE 144
           WEI+L  L   +  A G+YG +Y+G Y   +VA+KVL    + + S      L   F QE
Sbjct: 283 WEINLKHLKFGHKIASGSYGDLYKGTYCSQEVAIKVLK--PERLDS-----DLEKEFAQE 342

Query: 145 VAVWHKLDHPNVAKFYGASMGTSNLKIPPKSSSFDSNQTFPSRACCVVVEYLPGGTLKSF 204
           V +  K+ H NV +F GA     +L                    C+V E++PGG++  +
Sbjct: 343 VFIMRKVRHKNVVQFIGACTKPPHL--------------------CIVTEFMPGGSVYDY 402

Query: 205 LIKNRKRKLAFKI--VIQLALDLSRGLSYLHSKKIVHRDIKTENVLLDAQKTLKIVDFGV 264
           L    K+K  FK+  + ++A+D+ +G+SYLH   I+HRD+K  N+L+D  + +K+ DFGV
Sbjct: 403 L---HKQKGVFKLPTLFKVAIDICKGMSYLHQNNIIHRDLKAANLLMDENEVVKVADFGV 462

Query: 265 ARVEAQNPKDMTGETGTLGYMAPEVLDGKPYNRKCDVYSLGICLWETYCCDMPYPDLSFA 324
           ARV+AQ    MT ETGT  +MAPEV++ KPY+ K DV+S GI LWE     +PY  ++  
Sbjct: 463 ARVKAQTGV-MTAETGTYRWMAPEVIEHKPYDHKADVFSYGIVLWELLTGKLPYEYMTPL 522

Query: 325 EVSTAVVRQNLRPDIPRCCPSSFANIMKRCWDANPEKRPDMDEVVKLLEAIDTSKG 379
           + +  VV++ LRP IP+      A +++R W+ +  +RPD  E+++ L+ I    G
Sbjct: 523 QAAVGVVQKGLRPTIPKNTHPKLAELLERLWEHDSTQRPDFSEIIEQLQEIAKEVG 547

BLAST of CSPI07G16060 vs. ExPASy TrEMBL
Match: A0A0A0K8T2 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G387170 PE=4 SV=1)

HSP 1 Score: 817.8 bits (2111), Expect = 2.0e-233
Identity = 397/398 (99.75%), Postives = 398/398 (100.00%), Query Frame = 0

Query: 1   MDLANGGEAGKALVANQVADIVSGKSRNTQENDLGSKLGTGSKSNRDMVFRADKIDFKSW 60
           MDLANGGEAGKALVANQVADIVSGKSRNTQENDLGSKLGTGSKSNRDMVFRADKIDFKSW
Sbjct: 1   MDLANGGEAGKALVANQVADIVSGKSRNTQENDLGSKLGTGSKSNRDMVFRADKIDFKSW 60

Query: 61  DIQLEKHLSRAWSRDREVPAKKEEWEIDLSKLDIRYVKAHGTYGTIYRGNYDGNDVAVKV 120
           DIQLEKHLSRAWSRDREVPAKKEEWEIDLSKLDIRYVKAHGTYGTIYRGNYDGNDVAVKV
Sbjct: 61  DIQLEKHLSRAWSRDREVPAKKEEWEIDLSKLDIRYVKAHGTYGTIYRGNYDGNDVAVKV 120

Query: 121 LDWGEDGVSSVAEIAALRTSFRQEVAVWHKLDHPNVAKFYGASMGTSNLKIPPKSSSFDS 180
           LDWGEDGVSSVAEIAALRTSFRQEVAVWHKLDHPNVAKFYGASMGTSNLKIPPKSSSFDS
Sbjct: 121 LDWGEDGVSSVAEIAALRTSFRQEVAVWHKLDHPNVAKFYGASMGTSNLKIPPKSSSFDS 180

Query: 181 NQTFPSRACCVVVEYLPGGTLKSFLIKNRKRKLAFKIVIQLALDLSRGLSYLHSKKIVHR 240
           NQTFPSRACCVVVEYLPGGTLKSFLIKNRKRKLAFK+VIQLALDLSRGLSYLHSKKIVHR
Sbjct: 181 NQTFPSRACCVVVEYLPGGTLKSFLIKNRKRKLAFKVVIQLALDLSRGLSYLHSKKIVHR 240

Query: 241 DIKTENVLLDAQKTLKIVDFGVARVEAQNPKDMTGETGTLGYMAPEVLDGKPYNRKCDVY 300
           DIKTENVLLDAQKTLKIVDFGVARVEAQNPKDMTGETGTLGYMAPEVLDGKPYNRKCDVY
Sbjct: 241 DIKTENVLLDAQKTLKIVDFGVARVEAQNPKDMTGETGTLGYMAPEVLDGKPYNRKCDVY 300

Query: 301 SLGICLWETYCCDMPYPDLSFAEVSTAVVRQNLRPDIPRCCPSSFANIMKRCWDANPEKR 360
           SLGICLWETYCCDMPYPDLSFAEVSTAVVRQNLRPDIPRCCPSSFANIMKRCWDANPEKR
Sbjct: 301 SLGICLWETYCCDMPYPDLSFAEVSTAVVRQNLRPDIPRCCPSSFANIMKRCWDANPEKR 360

Query: 361 PDMDEVVKLLEAIDTSKGGGMITEDQISCFCFRPARGP 399
           PDMDEVVKLLEAIDTSKGGGMITEDQISCFCFRPARGP
Sbjct: 361 PDMDEVVKLLEAIDTSKGGGMITEDQISCFCFRPARGP 398

BLAST of CSPI07G16060 vs. ExPASy TrEMBL
Match: A0A5D3DRU0 (Serine/threonine-protein kinase HT1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold111G001020 PE=4 SV=1)

HSP 1 Score: 799.7 bits (2064), Expect = 5.7e-228
Identity = 388/398 (97.49%), Postives = 392/398 (98.49%), Query Frame = 0

Query: 1   MDLANGGEAGKALVANQVADIVSGKSRNTQENDLGSKLGTGSKSNRDMVFRADKIDFKSW 60
           MDLAN GE GKA VANQVADIVS KS NTQEN+LGSKLGTGSKSNRDMVFRADKIDFKSW
Sbjct: 1   MDLANAGEVGKAPVANQVADIVSAKSSNTQENELGSKLGTGSKSNRDMVFRADKIDFKSW 60

Query: 61  DIQLEKHLSRAWSRDREVPAKKEEWEIDLSKLDIRYVKAHGTYGTIYRGNYDGNDVAVKV 120
           DIQLEKHLSRAWSRDREVPAKKE WEIDLSKLDIRYVKAHGTYGTIYRGNYDGNDVAVKV
Sbjct: 61  DIQLEKHLSRAWSRDREVPAKKEVWEIDLSKLDIRYVKAHGTYGTIYRGNYDGNDVAVKV 120

Query: 121 LDWGEDGVSSVAEIAALRTSFRQEVAVWHKLDHPNVAKFYGASMGTSNLKIPPKSSSFDS 180
           LDWGEDGVSSVAEIAALRTSFRQEVAVWHKLDHPNVAKFYGASMGTSNLKIPPKSSSFDS
Sbjct: 121 LDWGEDGVSSVAEIAALRTSFRQEVAVWHKLDHPNVAKFYGASMGTSNLKIPPKSSSFDS 180

Query: 181 NQTFPSRACCVVVEYLPGGTLKSFLIKNRKRKLAFKIVIQLALDLSRGLSYLHSKKIVHR 240
           NQTFPSRACCVVVEYLPGGTLKSFLIKNRKRKLAFK+VIQLALDLSRGLSYLHSKKIVHR
Sbjct: 181 NQTFPSRACCVVVEYLPGGTLKSFLIKNRKRKLAFKVVIQLALDLSRGLSYLHSKKIVHR 240

Query: 241 DIKTENVLLDAQKTLKIVDFGVARVEAQNPKDMTGETGTLGYMAPEVLDGKPYNRKCDVY 300
           DIKTENVLLDAQKTLKIVDFGVARVEAQNPKDMTGETGTLGYMAPEVLDGKPYNRKCDVY
Sbjct: 241 DIKTENVLLDAQKTLKIVDFGVARVEAQNPKDMTGETGTLGYMAPEVLDGKPYNRKCDVY 300

Query: 301 SLGICLWETYCCDMPYPDLSFAEVSTAVVRQNLRPDIPRCCPSSFANIMKRCWDANPEKR 360
           SLGICLWETYCCDMPYPDLSFAEVSTAVVRQNLRP+IPRCCPSSFANIMKRCWDANPEKR
Sbjct: 301 SLGICLWETYCCDMPYPDLSFAEVSTAVVRQNLRPEIPRCCPSSFANIMKRCWDANPEKR 360

Query: 361 PDMDEVVKLLEAIDTSKGGGMITEDQISCFCFRPARGP 399
           PDMDEVVKLLEAIDTSKGGGMIT+DQISCFCFRPARGP
Sbjct: 361 PDMDEVVKLLEAIDTSKGGGMITDDQISCFCFRPARGP 398

BLAST of CSPI07G16060 vs. ExPASy TrEMBL
Match: A0A6J1JLG9 (serine/threonine-protein kinase HT1-like OS=Cucurbita maxima OX=3661 GN=LOC111488000 PE=4 SV=1)

HSP 1 Score: 748.8 bits (1932), Expect = 1.2e-212
Identity = 363/403 (90.07%), Postives = 380/403 (94.29%), Query Frame = 0

Query: 1   MDLANGGEAGKALVAN----QVADIVSGKSRNTQENDLGSKLGTGSKSNRDMVFRADKID 60
           MDL NGG AGKA +AN    +VADIVSGKS N QENDLGSKLG GSKSN+DMVFRADKID
Sbjct: 1   MDLTNGGGAGKAPIANNSGTEVADIVSGKSSNPQENDLGSKLGIGSKSNKDMVFRADKID 60

Query: 61  FKSWDIQLEKHLSRAWSRDREVPAKKEEWEIDLSKLDIRYVKAHGTYGTIYRGNYDGNDV 120
           FKSWDIQL++HLSRAWSRDREVPAKKEEWEI+LSKLDIRYVKAHGTYGTIYRGNYDG D 
Sbjct: 61  FKSWDIQLDRHLSRAWSRDREVPAKKEEWEINLSKLDIRYVKAHGTYGTIYRGNYDGKDA 120

Query: 121 AVKVLDWGEDGVSSVAEIAALRTSFRQEVAVWHKLDHPNVAKFYGASMGTSNLKIPPKSS 180
           AVKVLDWGEDGVSSVAEIAALR SFRQEVAVWHKLDHPNVAKFYGASMGTSNLKIPPKS 
Sbjct: 121 AVKVLDWGEDGVSSVAEIAALRASFRQEVAVWHKLDHPNVAKFYGASMGTSNLKIPPKSP 180

Query: 181 SFDSNQTFPSRACCVVVEYLPGGTLKSFLIKNRKRKLAFKIVIQLALDLSRGLSYLHSKK 240
           SFDSNQ+FPSRACCVVVEYLPGGTLK FLIKNRK+KLAFK+VIQLALDLSRGLSYLHSKK
Sbjct: 181 SFDSNQSFPSRACCVVVEYLPGGTLKGFLIKNRKKKLAFKVVIQLALDLSRGLSYLHSKK 240

Query: 241 IVHRDIKTENVLLDAQKTLKIVDFGVARVEAQNPKDMTGETGTLGYMAPEVLDGKPYNRK 300
           IVHRDIKTENVL DAQKTLKIVDFGVARVEAQNP+DMTGETGTLGYMAPEVLDGKPYNRK
Sbjct: 241 IVHRDIKTENVLFDAQKTLKIVDFGVARVEAQNPRDMTGETGTLGYMAPEVLDGKPYNRK 300

Query: 301 CDVYSLGICLWETYCCDMPYPDLSFAEVSTAVVRQNLRPDIPRCCPSSFANIMKRCWDAN 360
           CDVYS GICLWETYCCDMPYPDLSFAE+S+AVVRQNLRP+IPRCCPSSFA+IMKRCWDAN
Sbjct: 301 CDVYSFGICLWETYCCDMPYPDLSFAEISSAVVRQNLRPEIPRCCPSSFASIMKRCWDAN 360

Query: 361 PEKRPDMDEVVKLLEAIDTSKGGGMITEDQIS-CFCFRPARGP 399
           PE+RPDMDEVV LLEAIDTSKGGGMI+EDQ + CFCF   RGP
Sbjct: 361 PERRPDMDEVVNLLEAIDTSKGGGMISEDQATRCFCFGIPRGP 403

BLAST of CSPI07G16060 vs. ExPASy TrEMBL
Match: A0A6J1GNE5 (serine/threonine-protein kinase HT1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111456034 PE=4 SV=1)

HSP 1 Score: 746.9 bits (1927), Expect = 4.4e-212
Identity = 361/403 (89.58%), Postives = 380/403 (94.29%), Query Frame = 0

Query: 1   MDLANGGEAGKALVAN----QVADIVSGKSRNTQENDLGSKLGTGSKSNRDMVFRADKID 60
           MDL NGG AGKA +AN    +VADIVSGKS N QENDLGSKLG GSKSN+DMVFRADKID
Sbjct: 1   MDLTNGGRAGKAPIANNSVTEVADIVSGKSSNPQENDLGSKLGIGSKSNKDMVFRADKID 60

Query: 61  FKSWDIQLEKHLSRAWSRDREVPAKKEEWEIDLSKLDIRYVKAHGTYGTIYRGNYDGNDV 120
           FKSWDIQL++HLSRAWSRDREVPAKKEEWEI+LSKLDIRYVKAHGTYGTIYRGNYDG D 
Sbjct: 61  FKSWDIQLDRHLSRAWSRDREVPAKKEEWEINLSKLDIRYVKAHGTYGTIYRGNYDGKDA 120

Query: 121 AVKVLDWGEDGVSSVAEIAALRTSFRQEVAVWHKLDHPNVAKFYGASMGTSNLKIPPKSS 180
           AVKVLDWGEDGVSSVAEIAALR SFRQEVAVWH+LDHPNVAKFYGASMGTSNLKIPPKS 
Sbjct: 121 AVKVLDWGEDGVSSVAEIAALRASFRQEVAVWHRLDHPNVAKFYGASMGTSNLKIPPKSP 180

Query: 181 SFDSNQTFPSRACCVVVEYLPGGTLKSFLIKNRKRKLAFKIVIQLALDLSRGLSYLHSKK 240
           SFDSNQ+FPSRACCVVVEYLPGGTLK FLIKNRK+KLAFK+VIQLALDLSRGLSYLHSKK
Sbjct: 181 SFDSNQSFPSRACCVVVEYLPGGTLKGFLIKNRKKKLAFKVVIQLALDLSRGLSYLHSKK 240

Query: 241 IVHRDIKTENVLLDAQKTLKIVDFGVARVEAQNPKDMTGETGTLGYMAPEVLDGKPYNRK 300
           IVHRDIKTENVL DAQKTLKIVDFGVARVEAQNP+DMTGETGTLGYMAPEVLDGKPYNRK
Sbjct: 241 IVHRDIKTENVLFDAQKTLKIVDFGVARVEAQNPRDMTGETGTLGYMAPEVLDGKPYNRK 300

Query: 301 CDVYSLGICLWETYCCDMPYPDLSFAEVSTAVVRQNLRPDIPRCCPSSFANIMKRCWDAN 360
           CDVYS GICLWETYCCDMPYPDLSFAE+S+AVVRQNLRP+IPRCCPSSFA+IMKRCWDAN
Sbjct: 301 CDVYSFGICLWETYCCDMPYPDLSFAEISSAVVRQNLRPEIPRCCPSSFASIMKRCWDAN 360

Query: 361 PEKRPDMDEVVKLLEAIDTSKGGGMITEDQIS-CFCFRPARGP 399
           PE+RPDMDEVV LLEAIDTSKGGGMI++DQ + CFCF   RGP
Sbjct: 361 PERRPDMDEVVNLLEAIDTSKGGGMISKDQATRCFCFGIPRGP 403

BLAST of CSPI07G16060 vs. ExPASy TrEMBL
Match: A0A6J1H9S8 (serine/threonine-protein kinase HT1-like OS=Cucurbita moschata OX=3662 GN=LOC111460911 PE=4 SV=1)

HSP 1 Score: 726.1 bits (1873), Expect = 8.0e-206
Identity = 354/403 (87.84%), Postives = 372/403 (92.31%), Query Frame = 0

Query: 1   MDLANGGEAGKALV----ANQVADIVSGKSRNTQENDLGSKLGTGSKSNRDMVFRADKID 60
           MDL N GEA  A       NQVADIVSGK+ N  E DLGSKL TGSKS++DMVFRADKID
Sbjct: 1   MDLVNAGEASDAPAGKNSGNQVADIVSGKTSNPGEKDLGSKLETGSKSSKDMVFRADKID 60

Query: 61  FKSWDIQLEKHLSRAWSRDREVPAKKEEWEIDLSKLDIRYVKAHGTYGTIYRGNYDGNDV 120
           FK+WDIQL+KHLSRAWSRDREVPAKKEEWEIDLSKLDIRYVKAHGT+GTIYRGNYDGNDV
Sbjct: 61  FKNWDIQLDKHLSRAWSRDREVPAKKEEWEIDLSKLDIRYVKAHGTFGTIYRGNYDGNDV 120

Query: 121 AVKVLDWGEDGVSSVAEIAALRTSFRQEVAVWHKLDHPNVAKFYGASMGTSNLKIPPKSS 180
           AVKVLDWGEDGVSSVAEIAALRTSFRQEVAVWHKLDHPNVAKF+GASMGT NLKIP ++S
Sbjct: 121 AVKVLDWGEDGVSSVAEIAALRTSFRQEVAVWHKLDHPNVAKFFGASMGTYNLKIPSQNS 180

Query: 181 SFDSNQTFPSRACCVVVEYLPGGTLKSFLIKNRKRKLAFKIVIQLALDLSRGLSYLHSKK 240
           S D NQ+FPSRACCVVVEYLPGGTLK FLIKNRK+KLA K+VIQL LDLSRGLSYLHSKK
Sbjct: 181 SSDGNQSFPSRACCVVVEYLPGGTLKGFLIKNRKKKLALKVVIQLVLDLSRGLSYLHSKK 240

Query: 241 IVHRDIKTENVLLDAQKTLKIVDFGVARVEAQNPKDMTGETGTLGYMAPEVLDGKPYNRK 300
           IVHRDIKTENVLLDAQKTLKIVDFGVARVEAQNP+DMTGETGTLGYMAPEVLDGKPYNRK
Sbjct: 241 IVHRDIKTENVLLDAQKTLKIVDFGVARVEAQNPRDMTGETGTLGYMAPEVLDGKPYNRK 300

Query: 301 CDVYSLGICLWETYCCDMPYPDLSFAEVSTAVVRQNLRPDIPRCCPSSFANIMKRCWDAN 360
           CDVYS GICLWETYCCDMPYPDLSFAEVS+AVV+QNLRP+IPRCCPSS ANIM+RCWDAN
Sbjct: 301 CDVYSFGICLWETYCCDMPYPDLSFAEVSSAVVQQNLRPEIPRCCPSSLANIMRRCWDAN 360

Query: 361 PEKRPDMDEVVKLLEAIDTSKGGGMITEDQIS-CFCFRPARGP 399
           PE+RPDMDEVVKLLEAIDTSKGGGMI EDQ S CFCF   RGP
Sbjct: 361 PERRPDMDEVVKLLEAIDTSKGGGMIPEDQASRCFCFGKPRGP 403

BLAST of CSPI07G16060 vs. NCBI nr
Match: XP_004146993.1 (serine/threonine-protein kinase STY13 [Cucumis sativus])

HSP 1 Score: 817.8 bits (2111), Expect = 4.2e-233
Identity = 397/398 (99.75%), Postives = 398/398 (100.00%), Query Frame = 0

Query: 1   MDLANGGEAGKALVANQVADIVSGKSRNTQENDLGSKLGTGSKSNRDMVFRADKIDFKSW 60
           MDLANGGEAGKALVANQVADIVSGKSRNTQENDLGSKLGTGSKSNRDMVFRADKIDFKSW
Sbjct: 1   MDLANGGEAGKALVANQVADIVSGKSRNTQENDLGSKLGTGSKSNRDMVFRADKIDFKSW 60

Query: 61  DIQLEKHLSRAWSRDREVPAKKEEWEIDLSKLDIRYVKAHGTYGTIYRGNYDGNDVAVKV 120
           DIQLEKHLSRAWSRDREVPAKKEEWEIDLSKLDIRYVKAHGTYGTIYRGNYDGNDVAVKV
Sbjct: 61  DIQLEKHLSRAWSRDREVPAKKEEWEIDLSKLDIRYVKAHGTYGTIYRGNYDGNDVAVKV 120

Query: 121 LDWGEDGVSSVAEIAALRTSFRQEVAVWHKLDHPNVAKFYGASMGTSNLKIPPKSSSFDS 180
           LDWGEDGVSSVAEIAALRTSFRQEVAVWHKLDHPNVAKFYGASMGTSNLKIPPKSSSFDS
Sbjct: 121 LDWGEDGVSSVAEIAALRTSFRQEVAVWHKLDHPNVAKFYGASMGTSNLKIPPKSSSFDS 180

Query: 181 NQTFPSRACCVVVEYLPGGTLKSFLIKNRKRKLAFKIVIQLALDLSRGLSYLHSKKIVHR 240
           NQTFPSRACCVVVEYLPGGTLKSFLIKNRKRKLAFK+VIQLALDLSRGLSYLHSKKIVHR
Sbjct: 181 NQTFPSRACCVVVEYLPGGTLKSFLIKNRKRKLAFKVVIQLALDLSRGLSYLHSKKIVHR 240

Query: 241 DIKTENVLLDAQKTLKIVDFGVARVEAQNPKDMTGETGTLGYMAPEVLDGKPYNRKCDVY 300
           DIKTENVLLDAQKTLKIVDFGVARVEAQNPKDMTGETGTLGYMAPEVLDGKPYNRKCDVY
Sbjct: 241 DIKTENVLLDAQKTLKIVDFGVARVEAQNPKDMTGETGTLGYMAPEVLDGKPYNRKCDVY 300

Query: 301 SLGICLWETYCCDMPYPDLSFAEVSTAVVRQNLRPDIPRCCPSSFANIMKRCWDANPEKR 360
           SLGICLWETYCCDMPYPDLSFAEVSTAVVRQNLRPDIPRCCPSSFANIMKRCWDANPEKR
Sbjct: 301 SLGICLWETYCCDMPYPDLSFAEVSTAVVRQNLRPDIPRCCPSSFANIMKRCWDANPEKR 360

Query: 361 PDMDEVVKLLEAIDTSKGGGMITEDQISCFCFRPARGP 399
           PDMDEVVKLLEAIDTSKGGGMITEDQISCFCFRPARGP
Sbjct: 361 PDMDEVVKLLEAIDTSKGGGMITEDQISCFCFRPARGP 398

BLAST of CSPI07G16060 vs. NCBI nr
Match: KAA0064024.1 (serine/threonine-protein kinase HT1 [Cucumis melo var. makuwa] >TYK26175.1 serine/threonine-protein kinase HT1 [Cucumis melo var. makuwa])

HSP 1 Score: 799.7 bits (2064), Expect = 1.2e-227
Identity = 388/398 (97.49%), Postives = 392/398 (98.49%), Query Frame = 0

Query: 1   MDLANGGEAGKALVANQVADIVSGKSRNTQENDLGSKLGTGSKSNRDMVFRADKIDFKSW 60
           MDLAN GE GKA VANQVADIVS KS NTQEN+LGSKLGTGSKSNRDMVFRADKIDFKSW
Sbjct: 1   MDLANAGEVGKAPVANQVADIVSAKSSNTQENELGSKLGTGSKSNRDMVFRADKIDFKSW 60

Query: 61  DIQLEKHLSRAWSRDREVPAKKEEWEIDLSKLDIRYVKAHGTYGTIYRGNYDGNDVAVKV 120
           DIQLEKHLSRAWSRDREVPAKKE WEIDLSKLDIRYVKAHGTYGTIYRGNYDGNDVAVKV
Sbjct: 61  DIQLEKHLSRAWSRDREVPAKKEVWEIDLSKLDIRYVKAHGTYGTIYRGNYDGNDVAVKV 120

Query: 121 LDWGEDGVSSVAEIAALRTSFRQEVAVWHKLDHPNVAKFYGASMGTSNLKIPPKSSSFDS 180
           LDWGEDGVSSVAEIAALRTSFRQEVAVWHKLDHPNVAKFYGASMGTSNLKIPPKSSSFDS
Sbjct: 121 LDWGEDGVSSVAEIAALRTSFRQEVAVWHKLDHPNVAKFYGASMGTSNLKIPPKSSSFDS 180

Query: 181 NQTFPSRACCVVVEYLPGGTLKSFLIKNRKRKLAFKIVIQLALDLSRGLSYLHSKKIVHR 240
           NQTFPSRACCVVVEYLPGGTLKSFLIKNRKRKLAFK+VIQLALDLSRGLSYLHSKKIVHR
Sbjct: 181 NQTFPSRACCVVVEYLPGGTLKSFLIKNRKRKLAFKVVIQLALDLSRGLSYLHSKKIVHR 240

Query: 241 DIKTENVLLDAQKTLKIVDFGVARVEAQNPKDMTGETGTLGYMAPEVLDGKPYNRKCDVY 300
           DIKTENVLLDAQKTLKIVDFGVARVEAQNPKDMTGETGTLGYMAPEVLDGKPYNRKCDVY
Sbjct: 241 DIKTENVLLDAQKTLKIVDFGVARVEAQNPKDMTGETGTLGYMAPEVLDGKPYNRKCDVY 300

Query: 301 SLGICLWETYCCDMPYPDLSFAEVSTAVVRQNLRPDIPRCCPSSFANIMKRCWDANPEKR 360
           SLGICLWETYCCDMPYPDLSFAEVSTAVVRQNLRP+IPRCCPSSFANIMKRCWDANPEKR
Sbjct: 301 SLGICLWETYCCDMPYPDLSFAEVSTAVVRQNLRPEIPRCCPSSFANIMKRCWDANPEKR 360

Query: 361 PDMDEVVKLLEAIDTSKGGGMITEDQISCFCFRPARGP 399
           PDMDEVVKLLEAIDTSKGGGMIT+DQISCFCFRPARGP
Sbjct: 361 PDMDEVVKLLEAIDTSKGGGMITDDQISCFCFRPARGP 398

BLAST of CSPI07G16060 vs. NCBI nr
Match: XP_038898730.1 (serine/threonine-protein kinase STY13-like [Benincasa hispida])

HSP 1 Score: 773.5 bits (1996), Expect = 9.0e-220
Identity = 376/402 (93.53%), Postives = 384/402 (95.52%), Query Frame = 0

Query: 1   MDLANGGEAGKALVA----NQVADIVSGKSRNTQENDLGSKLGTGSKSNRDMVFRADKID 60
           MD  NGGE G A VA    NQVADIVSGKS N QE DLGSKLGTGSKSNRDMVFRADKID
Sbjct: 1   MDSTNGGEVGNAPVAKNYGNQVADIVSGKSSNIQEKDLGSKLGTGSKSNRDMVFRADKID 60

Query: 61  FKSWDIQLEKHLSRAWSRDREVPAKKEEWEIDLSKLDIRYVKAHGTYGTIYRGNYDGNDV 120
           FKSWDIQLEKHLSRAWSRDREVPAKKEEWEIDLSKLDIRYVKAHGTYGTIYRGNYDGNDV
Sbjct: 61  FKSWDIQLEKHLSRAWSRDREVPAKKEEWEIDLSKLDIRYVKAHGTYGTIYRGNYDGNDV 120

Query: 121 AVKVLDWGEDGVSSVAEIAALRTSFRQEVAVWHKLDHPNVAKFYGASMGTSNLKIPPKSS 180
           AVKVLDWGEDGV+SVAEIAALRTSFRQEVAVWHKLDHPNVAKFYGASMGTSNLKIPPKSS
Sbjct: 121 AVKVLDWGEDGVASVAEIAALRTSFRQEVAVWHKLDHPNVAKFYGASMGTSNLKIPPKSS 180

Query: 181 SFDSNQTFPSRACCVVVEYLPGGTLKSFLIKNRKRKLAFKIVIQLALDLSRGLSYLHSKK 240
           SFDSNQ+FPSRACCVVVEYLPGGTLKSFLIKNRK+KLAFK+VIQLALDLSRGLSYLHSKK
Sbjct: 181 SFDSNQSFPSRACCVVVEYLPGGTLKSFLIKNRKKKLAFKVVIQLALDLSRGLSYLHSKK 240

Query: 241 IVHRDIKTENVLLDAQKTLKIVDFGVARVEAQNPKDMTGETGTLGYMAPEVLDGKPYNRK 300
           IVHRDIKTENVLLD QKTLKIVDFGVARVEAQNP+DMTGETGTLGYMAPEVLDGKPYNRK
Sbjct: 241 IVHRDIKTENVLLDDQKTLKIVDFGVARVEAQNPRDMTGETGTLGYMAPEVLDGKPYNRK 300

Query: 301 CDVYSLGICLWETYCCDMPYPDLSFAEVSTAVVRQNLRPDIPRCCPSSFANIMKRCWDAN 360
           CDVYS GICLWETYCCDMPYPDLSFAEVS+AVVRQNLRPDIPRCCPSSF+NIMKRCWDAN
Sbjct: 301 CDVYSFGICLWETYCCDMPYPDLSFAEVSSAVVRQNLRPDIPRCCPSSFSNIMKRCWDAN 360

Query: 361 PEKRPDMDEVVKLLEAIDTSKGGGMITEDQISCFCFRPARGP 399
           PE+RPDMDEVVKLLEAIDTSKGGGMI EDQ SCFCFR  RGP
Sbjct: 361 PERRPDMDEVVKLLEAIDTSKGGGMINEDQTSCFCFRTPRGP 402

BLAST of CSPI07G16060 vs. NCBI nr
Match: XP_022991317.1 (serine/threonine-protein kinase HT1-like [Cucurbita maxima])

HSP 1 Score: 748.8 bits (1932), Expect = 2.4e-212
Identity = 363/403 (90.07%), Postives = 380/403 (94.29%), Query Frame = 0

Query: 1   MDLANGGEAGKALVAN----QVADIVSGKSRNTQENDLGSKLGTGSKSNRDMVFRADKID 60
           MDL NGG AGKA +AN    +VADIVSGKS N QENDLGSKLG GSKSN+DMVFRADKID
Sbjct: 1   MDLTNGGGAGKAPIANNSGTEVADIVSGKSSNPQENDLGSKLGIGSKSNKDMVFRADKID 60

Query: 61  FKSWDIQLEKHLSRAWSRDREVPAKKEEWEIDLSKLDIRYVKAHGTYGTIYRGNYDGNDV 120
           FKSWDIQL++HLSRAWSRDREVPAKKEEWEI+LSKLDIRYVKAHGTYGTIYRGNYDG D 
Sbjct: 61  FKSWDIQLDRHLSRAWSRDREVPAKKEEWEINLSKLDIRYVKAHGTYGTIYRGNYDGKDA 120

Query: 121 AVKVLDWGEDGVSSVAEIAALRTSFRQEVAVWHKLDHPNVAKFYGASMGTSNLKIPPKSS 180
           AVKVLDWGEDGVSSVAEIAALR SFRQEVAVWHKLDHPNVAKFYGASMGTSNLKIPPKS 
Sbjct: 121 AVKVLDWGEDGVSSVAEIAALRASFRQEVAVWHKLDHPNVAKFYGASMGTSNLKIPPKSP 180

Query: 181 SFDSNQTFPSRACCVVVEYLPGGTLKSFLIKNRKRKLAFKIVIQLALDLSRGLSYLHSKK 240
           SFDSNQ+FPSRACCVVVEYLPGGTLK FLIKNRK+KLAFK+VIQLALDLSRGLSYLHSKK
Sbjct: 181 SFDSNQSFPSRACCVVVEYLPGGTLKGFLIKNRKKKLAFKVVIQLALDLSRGLSYLHSKK 240

Query: 241 IVHRDIKTENVLLDAQKTLKIVDFGVARVEAQNPKDMTGETGTLGYMAPEVLDGKPYNRK 300
           IVHRDIKTENVL DAQKTLKIVDFGVARVEAQNP+DMTGETGTLGYMAPEVLDGKPYNRK
Sbjct: 241 IVHRDIKTENVLFDAQKTLKIVDFGVARVEAQNPRDMTGETGTLGYMAPEVLDGKPYNRK 300

Query: 301 CDVYSLGICLWETYCCDMPYPDLSFAEVSTAVVRQNLRPDIPRCCPSSFANIMKRCWDAN 360
           CDVYS GICLWETYCCDMPYPDLSFAE+S+AVVRQNLRP+IPRCCPSSFA+IMKRCWDAN
Sbjct: 301 CDVYSFGICLWETYCCDMPYPDLSFAEISSAVVRQNLRPEIPRCCPSSFASIMKRCWDAN 360

Query: 361 PEKRPDMDEVVKLLEAIDTSKGGGMITEDQIS-CFCFRPARGP 399
           PE+RPDMDEVV LLEAIDTSKGGGMI+EDQ + CFCF   RGP
Sbjct: 361 PERRPDMDEVVNLLEAIDTSKGGGMISEDQATRCFCFGIPRGP 403

BLAST of CSPI07G16060 vs. NCBI nr
Match: XP_022953502.1 (serine/threonine-protein kinase HT1-like isoform X1 [Cucurbita moschata] >XP_022953503.1 serine/threonine-protein kinase HT1-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 746.9 bits (1927), Expect = 9.1e-212
Identity = 361/403 (89.58%), Postives = 380/403 (94.29%), Query Frame = 0

Query: 1   MDLANGGEAGKALVAN----QVADIVSGKSRNTQENDLGSKLGTGSKSNRDMVFRADKID 60
           MDL NGG AGKA +AN    +VADIVSGKS N QENDLGSKLG GSKSN+DMVFRADKID
Sbjct: 1   MDLTNGGRAGKAPIANNSVTEVADIVSGKSSNPQENDLGSKLGIGSKSNKDMVFRADKID 60

Query: 61  FKSWDIQLEKHLSRAWSRDREVPAKKEEWEIDLSKLDIRYVKAHGTYGTIYRGNYDGNDV 120
           FKSWDIQL++HLSRAWSRDREVPAKKEEWEI+LSKLDIRYVKAHGTYGTIYRGNYDG D 
Sbjct: 61  FKSWDIQLDRHLSRAWSRDREVPAKKEEWEINLSKLDIRYVKAHGTYGTIYRGNYDGKDA 120

Query: 121 AVKVLDWGEDGVSSVAEIAALRTSFRQEVAVWHKLDHPNVAKFYGASMGTSNLKIPPKSS 180
           AVKVLDWGEDGVSSVAEIAALR SFRQEVAVWH+LDHPNVAKFYGASMGTSNLKIPPKS 
Sbjct: 121 AVKVLDWGEDGVSSVAEIAALRASFRQEVAVWHRLDHPNVAKFYGASMGTSNLKIPPKSP 180

Query: 181 SFDSNQTFPSRACCVVVEYLPGGTLKSFLIKNRKRKLAFKIVIQLALDLSRGLSYLHSKK 240
           SFDSNQ+FPSRACCVVVEYLPGGTLK FLIKNRK+KLAFK+VIQLALDLSRGLSYLHSKK
Sbjct: 181 SFDSNQSFPSRACCVVVEYLPGGTLKGFLIKNRKKKLAFKVVIQLALDLSRGLSYLHSKK 240

Query: 241 IVHRDIKTENVLLDAQKTLKIVDFGVARVEAQNPKDMTGETGTLGYMAPEVLDGKPYNRK 300
           IVHRDIKTENVL DAQKTLKIVDFGVARVEAQNP+DMTGETGTLGYMAPEVLDGKPYNRK
Sbjct: 241 IVHRDIKTENVLFDAQKTLKIVDFGVARVEAQNPRDMTGETGTLGYMAPEVLDGKPYNRK 300

Query: 301 CDVYSLGICLWETYCCDMPYPDLSFAEVSTAVVRQNLRPDIPRCCPSSFANIMKRCWDAN 360
           CDVYS GICLWETYCCDMPYPDLSFAE+S+AVVRQNLRP+IPRCCPSSFA+IMKRCWDAN
Sbjct: 301 CDVYSFGICLWETYCCDMPYPDLSFAEISSAVVRQNLRPEIPRCCPSSFASIMKRCWDAN 360

Query: 361 PEKRPDMDEVVKLLEAIDTSKGGGMITEDQIS-CFCFRPARGP 399
           PE+RPDMDEVV LLEAIDTSKGGGMI++DQ + CFCF   RGP
Sbjct: 361 PERRPDMDEVVNLLEAIDTSKGGGMISKDQATRCFCFGIPRGP 403

BLAST of CSPI07G16060 vs. TAIR 10
Match: AT3G22750.1 (Protein kinase superfamily protein )

HSP 1 Score: 582.4 bits (1500), Expect = 2.8e-166
Identity = 274/376 (72.87%), Postives = 321/376 (85.37%), Query Frame = 0

Query: 25  KSRNTQENDLGSKLGTGSKSNRDMVFRADKIDFKSWDIQLEKHLSRAWSRDREV-PAKKE 84
           +++ + EN+L ++   G+ S +DM+FRADKID K+ DIQLEKHLSR WSR  E  P  KE
Sbjct: 6   ETKASPENNLRNRGADGNNSKKDMIFRADKIDLKNLDIQLEKHLSRVWSRSIEKHPKPKE 65

Query: 85  EWEIDLSKLDIRYVKAHGTYGTIYRGNYDGNDVAVKVLDWGEDGVSSVAEIAALRTSFRQ 144
           EWEI+L+KL++R V A G YG +Y+G YDG DVAVKVLDWGEDG ++ AE +ALR SFRQ
Sbjct: 66  EWEIELAKLEMRNVIARGAYGIVYKGIYDGQDVAVKVLDWGEDGYATTAETSALRASFRQ 125

Query: 145 EVAVWHKLDHPNVAKFYGASMGTSNLKIPPKSSSFDSNQTFPSRACCVVVEYLPGGTLKS 204
           EVAVWHKLDHPNV +F GASMGT+NLKIP   SS ++  + P RACCVVVEY+PGGTLK 
Sbjct: 126 EVAVWHKLDHPNVTRFVGASMGTANLKIP---SSAETENSLPQRACCVVVEYIPGGTLKQ 185

Query: 205 FLIKNRKRKLAFKIVIQLALDLSRGLSYLHSKKIVHRDIKTENVLLDAQKTLKIVDFGVA 264
           +L +NR++KLAFK+V+QLALDLSRGLSYLHS++IVHRD+KTEN+LLD Q+ LKI DFGVA
Sbjct: 186 YLFRNRRKKLAFKVVVQLALDLSRGLSYLHSERIVHRDVKTENMLLDYQRNLKIADFGVA 245

Query: 265 RVEAQNPKDMTGETGTLGYMAPEVLDGKPYNRKCDVYSLGICLWETYCCDMPYPDLSFAE 324
           RVEAQNPKDMTGETGTLGYMAPEVLDGKPYNR+CDVYS GICLWE YCCDMPYPDLSFA+
Sbjct: 246 RVEAQNPKDMTGETGTLGYMAPEVLDGKPYNRRCDVYSFGICLWEIYCCDMPYPDLSFAD 305

Query: 325 VSTAVVRQNLRPDIPRCCPSSFANIMKRCWDANPEKRPDMDEVVKLLEAIDTSKGGGMIT 384
           VS+AVVRQNLRPDIPRCCP++ A IMKRCW+ANPEKRP+M+EVV LLEA+DT+KGGGMI 
Sbjct: 306 VSSAVVRQNLRPDIPRCCPTALATIMKRCWEANPEKRPEMEEVVSLLEAVDTTKGGGMIP 365

Query: 385 EDQ-ISCFCFRPARGP 399
           EDQ   CFCF   RGP
Sbjct: 366 EDQRPGCFCFVSGRGP 378

BLAST of CSPI07G16060 vs. TAIR 10
Match: AT4G14780.1 (Protein kinase superfamily protein )

HSP 1 Score: 544.7 bits (1402), Expect = 6.4e-155
Identity = 259/360 (71.94%), Postives = 296/360 (82.22%), Query Frame = 0

Query: 41  GSKSNRDMVFRADKIDFKSWDIQLEKHLSRAWSRDREV-PAKKEEWEIDLSKLDIRYVKA 100
           G+ + ++ +FRADKID KS D QLEKHLSR WSR+ EV P  KEEWEIDL+KL+   V A
Sbjct: 9   GNNTTKEKIFRADKIDLKSLDRQLEKHLSRVWSRNLEVNPKAKEEWEIDLAKLETSNVIA 68

Query: 101 HGTYGTIYRGNYDGNDVAVKVLDWGEDGVSSVAEIAALRTSFRQEVAVWHKLDHPNVAKF 160
            GTYGT+Y+G YDG DVAVKVLDW +DG  + A+ A  R  FRQEV VWHKL+HPNV KF
Sbjct: 69  RGTYGTVYKGIYDGQDVAVKVLDWEDDGNETTAKTATNRALFRQEVTVWHKLNHPNVTKF 128

Query: 161 YGASMGTSNLKIPPKSSSFDSNQTFPSRACCVVVEYLPGGTLKSFLIKNRKRKLAFKIVI 220
            GASMGT+NL I     S DS  + P +ACCVVVEYLPGGTLK  LI+++ +KLAFK VI
Sbjct: 129 VGASMGTTNLNI----RSADSKGSLPQQACCVVVEYLPGGTLKQHLIRHKSKKLAFKAVI 188

Query: 221 QLALDLSRGLSYLHSKKIVHRDIKTENVLLDAQKTLKIVDFGVARVEAQNPKDMTGETGT 280
           +LALDL+RGLSYLHS+KIVHRD+KTEN+LLDAQK LKI DFGVARVEA NPKDMTGETGT
Sbjct: 189 KLALDLARGLSYLHSEKIVHRDVKTENMLLDAQKNLKIADFGVARVEALNPKDMTGETGT 248

Query: 281 LGYMAPEVLDGKPYNRKCDVYSLGICLWETYCCDMPYPDLSFAEVSTAVVRQNLRPDIPR 340
           LGYMAPEV+DGKPYNR+CDVYS GICLWE YCCDMPYPDLSF +VS+AVV  NLRP+IPR
Sbjct: 249 LGYMAPEVIDGKPYNRRCDVYSFGICLWEIYCCDMPYPDLSFVDVSSAVVLHNLRPEIPR 308

Query: 341 CCPSSFANIMKRCWDANPEKRPDMDEVVKLLEAIDTSKGGGMITEDQI-SCFCFRPARGP 399
           CCP++ A IMK CWD NP+KRP+M EVVK+LE +DTSKGGGMI EDQ   CFCF PARGP
Sbjct: 309 CCPTALAGIMKTCWDGNPQKRPEMKEVVKMLEGVDTSKGGGMIPEDQSRGCFCFAPARGP 364

BLAST of CSPI07G16060 vs. TAIR 10
Match: AT3G63260.1 (Protein kinase superfamily protein )

HSP 1 Score: 518.1 bits (1333), Expect = 6.4e-147
Identity = 262/403 (65.01%), Postives = 304/403 (75.43%), Query Frame = 0

Query: 4   ANGGEAGKALVANQVADIVSGKSRNTQENDLGSKLGTGSKS-NRDMVFRADKIDFKSWDI 63
           + GGEA K+L      +I SG    T +  +G   GTGS+S   +  FRAD +DF  WD+
Sbjct: 3   SGGGEADKSL------EIGSG----TADPKIG---GTGSRSAGEERYFRADTLDFSKWDL 62

Query: 64  QL-----EKHLSRAWSRDREVPAKKEEWEIDLSKLDIRYVKAHGTYGTIYRGNYDGNDVA 123
            +        L+ + S     PA  +EWEIDLSKLD+++V AHGTYGT+YRG Y G +VA
Sbjct: 63  HMGQTSTSSVLTNSASTSAPAPA-MQEWEIDLSKLDMKHVLAHGTYGTVYRGVYAGQEVA 122

Query: 124 VKVLDWGEDGVSSVAEIAALRTSFRQEVAVWHKLDHPNVAKFYGASMGTSNLKIPPKSSS 183
           VKVLDWGEDG ++ AE  ALR SF QEVAVW KLDHPNV KF GASMGTS+L+IPP   +
Sbjct: 123 VKVLDWGEDGYATPAETTALRASFEQEVAVWQKLDHPNVTKFIGASMGTSDLRIPPAGDT 182

Query: 184 FD-SNQTFPSRACCVVVEYLPGGTLKSFLIKNRKRKLAFKIVIQLALDLSRGLSYLHSKK 243
               N   P+RACCVVVEY+ GGTLK FLIK  + KL  K VIQLALDL+RGLSYLHSK 
Sbjct: 183 GGRGNGAHPARACCVVVEYVAGGTLKKFLIKKYRAKLPIKDVIQLALDLARGLSYLHSKA 242

Query: 244 IVHRDIKTENVLLDAQKTLKIVDFGVARVEAQNPKDMTGETGTLGYMAPEVLDGKPYNRK 303
           IVHRD+K+EN+LL   KTLKI DFGVARVEAQNP+DMTGETGTLGYMAPEVL+GKPYNRK
Sbjct: 243 IVHRDVKSENMLLQPNKTLKIADFGVARVEAQNPQDMTGETGTLGYMAPEVLEGKPYNRK 302

Query: 304 CDVYSLGICLWETYCCDMPYPDLSFAEVSTAVVRQNLRPDIPRCCPSSFANIMKRCWDAN 363
           CDVYS G+CLWE YCCDMPY D SFAE+S AVV +NLRP+IP+CCP + ANIMKRCWD N
Sbjct: 303 CDVYSFGVCLWEIYCCDMPYADCSFAEISHAVVHRNLRPEIPKCCPHAVANIMKRCWDPN 362

Query: 364 PEKRPDMDEVVKLLEAIDTSKGGGMITEDQI-SCFCFRPARGP 399
           P++RP+M+EVVKLLEAIDTSKGGGMI  DQ   C CF   RGP
Sbjct: 363 PDRRPEMEEVVKLLEAIDTSKGGGMIAPDQFQGCLCFFKPRGP 391

BLAST of CSPI07G16060 vs. TAIR 10
Match: AT3G01490.1 (Protein kinase superfamily protein )

HSP 1 Score: 514.6 bits (1324), Expect = 7.0e-146
Identity = 255/401 (63.59%), Postives = 291/401 (72.57%), Query Frame = 0

Query: 51  RADKIDFKSWDIQLEKHLSRAWS------------------------------------- 110
           RAD+ID KS D QL++HLS+AW+                                     
Sbjct: 15  RADQIDLKSLDEQLQRHLSKAWTMEKRKSLSDGEDNVNNTRHNQNNFGHRQLVFQRPLLG 74

Query: 111 ---------------RDREVPAKKEEWEIDLSKLDIRYVKAHGTYGTIYRGNYDGNDVAV 170
                          R  EV   + EWEID SKL I+ V A GT+GT++RG YDG DVAV
Sbjct: 75  GGYSNNNNSSKNDIIRSTEVEKSRREWEIDPSKLIIKSVIARGTFGTVHRGIYDGQDVAV 134

Query: 171 KVLDWGEDGVSSVAEIAALRTSFRQEVAVWHKLDHPNVAKFYGASMGTSNLKIPPKSSSF 230
           K+LDWGE+G  S AEIA+LR +F QEVAVWHKLDHPNV KF GA+MGTS + I  ++   
Sbjct: 135 KLLDWGEEGHRSDAEIASLRAAFTQEVAVWHKLDHPNVTKFIGAAMGTSEMSIQTENGQM 194

Query: 231 DSNQTFPSRACCVVVEYLPGGTLKSFLIKNRKRKLAFKIVIQLALDLSRGLSYLHSKKIV 290
                 PS  CCVVVEY PGG LKSFLIK R+RKLAFK+VIQL+LDL+RGLSYLHS+KIV
Sbjct: 195 G----MPSNVCCVVVEYCPGGALKSFLIKTRRRKLAFKVVIQLSLDLARGLSYLHSQKIV 254

Query: 291 HRDIKTENVLLDAQKTLKIVDFGVARVEAQNPKDMTGETGTLGYMAPEVLDGKPYNRKCD 350
           HRD+KTEN+LLD  +TLKI DFGVAR+EA NP DMTGETGTLGYMAPEVL+G PYNRKCD
Sbjct: 255 HRDVKTENMLLDKSRTLKIADFGVARLEASNPNDMTGETGTLGYMAPEVLNGSPYNRKCD 314

Query: 351 VYSLGICLWETYCCDMPYPDLSFAEVSTAVVRQNLRPDIPRCCPSSFANIMKRCWDANPE 399
           VYS GICLWE YCCDMPYPDLSF+EV++AVVRQNLRP+IPRCCPSS AN+MKRCWDANPE
Sbjct: 315 VYSFGICLWEIYCCDMPYPDLSFSEVTSAVVRQNLRPEIPRCCPSSLANVMKRCWDANPE 374

BLAST of CSPI07G16060 vs. TAIR 10
Match: AT5G50000.1 (Protein kinase superfamily protein )

HSP 1 Score: 510.8 bits (1314), Expect = 1.0e-144
Identity = 250/388 (64.43%), Postives = 290/388 (74.74%), Query Frame = 0

Query: 43  KSNRDMVFRADKIDFKSWDIQLEKHLSRAWSRDREVPAKKE------------------- 102
           K  +D   RAD+ID KS D QLE+HLSRA + ++     +E                   
Sbjct: 2   KEGKDGFVRADQIDLKSLDEQLERHLSRALTLEKNKKKDEEDTTAVAIGGSASSSPVTLN 61

Query: 103 ------------EWEIDLSKLDIRYVKAHGTYGTIYRGNYDGNDVAVKVLDWGEDGVSSV 162
                       EWEID SKL I+ V A GT+GT++RG YDG DVAVK+LDWGE+G  S 
Sbjct: 62  GGGFVGKRKQRLEWEIDPSKLIIKTVLARGTFGTVHRGIYDGQDVAVKLLDWGEEGHRSE 121

Query: 163 AEIAALRTSFRQEVAVWHKLDHPNVAKFYGASMGTSNLKIPPKSSSFDSNQTFPSRACCV 222
           AEI +LR  F QEVAVWHKLDHPNV KF GA+MG S L++  +S         P+  CCV
Sbjct: 122 AEIVSLRADFAQEVAVWHKLDHPNVTKFIGATMGASGLQLQTESGPL----AMPNNICCV 181

Query: 223 VVEYLPGGTLKSFLIKNRKRKLAFKIVIQLALDLSRGLSYLHSKKIVHRDIKTENVLLDA 282
           VVEYLPGG LKS+LIKNR+RKL FKIV+QLALDL+RGLSYLHS+KIVHRD+KTEN+LLD 
Sbjct: 182 VVEYLPGGALKSYLIKNRRRKLTFKIVVQLALDLARGLSYLHSQKIVHRDVKTENMLLDK 241

Query: 283 QKTLKIVDFGVARVEAQNPKDMTGETGTLGYMAPEVLDGKPYNRKCDVYSLGICLWETYC 342
            +T+KI DFGVARVEA NP DMTGETGTLGYMAPEVL+G PYNRKCDVYS GICLWE YC
Sbjct: 242 TRTVKIADFGVARVEASNPNDMTGETGTLGYMAPEVLNGNPYNRKCDVYSFGICLWEIYC 301

Query: 343 CDMPYPDLSFAEVSTAVVRQNLRPDIPRCCPSSFANIMKRCWDANPEKRPDMDEVVKLLE 399
           CDMPYPDL+F+EV++AVVRQNLRPDIPRCCPS+ A +MKRCWDANP+KRP+MDEVV +LE
Sbjct: 302 CDMPYPDLTFSEVTSAVVRQNLRPDIPRCCPSALAAVMKRCWDANPDKRPEMDEVVPMLE 361

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9ZQ319.5e-6340.27Serine/threonine-protein kinase STY13 OS=Arabidopsis thaliana OX=3702 GN=STY13 P... [more]
Q2MHE47.3e-5536.59Serine/threonine/tyrosine-protein kinase HT1 OS=Arabidopsis thaliana OX=3702 GN=... [more]
O225581.4e-5338.00Serine/threonine-protein kinase STY8 OS=Arabidopsis thaliana OX=3702 GN=STY8 PE=... [more]
Q8RWL61.3e-5136.33Serine/threonine-protein kinase STY17 OS=Arabidopsis thaliana OX=3702 GN=STY17 P... [more]
F4JTP58.3e-5136.82Serine/threonine-protein kinase STY46 OS=Arabidopsis thaliana OX=3702 GN=STY46 P... [more]
Match NameE-valueIdentityDescription
A0A0A0K8T22.0e-23399.75Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G387... [more]
A0A5D3DRU05.7e-22897.49Serine/threonine-protein kinase HT1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5... [more]
A0A6J1JLG91.2e-21290.07serine/threonine-protein kinase HT1-like OS=Cucurbita maxima OX=3661 GN=LOC11148... [more]
A0A6J1GNE54.4e-21289.58serine/threonine-protein kinase HT1-like isoform X1 OS=Cucurbita moschata OX=366... [more]
A0A6J1H9S88.0e-20687.84serine/threonine-protein kinase HT1-like OS=Cucurbita moschata OX=3662 GN=LOC111... [more]
Match NameE-valueIdentityDescription
XP_004146993.14.2e-23399.75serine/threonine-protein kinase STY13 [Cucumis sativus][more]
KAA0064024.11.2e-22797.49serine/threonine-protein kinase HT1 [Cucumis melo var. makuwa] >TYK26175.1 serin... [more]
XP_038898730.19.0e-22093.53serine/threonine-protein kinase STY13-like [Benincasa hispida][more]
XP_022991317.12.4e-21290.07serine/threonine-protein kinase HT1-like [Cucurbita maxima][more]
XP_022953502.19.1e-21289.58serine/threonine-protein kinase HT1-like isoform X1 [Cucurbita moschata] >XP_022... [more]
Match NameE-valueIdentityDescription
AT3G22750.12.8e-16672.87Protein kinase superfamily protein [more]
AT4G14780.16.4e-15571.94Protein kinase superfamily protein [more]
AT3G63260.16.4e-14765.01Protein kinase superfamily protein [more]
AT3G01490.17.0e-14663.59Protein kinase superfamily protein [more]
AT5G50000.11.0e-14464.43Protein kinase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001245Serine-threonine/tyrosine-protein kinase, catalytic domainPRINTSPR00109TYRKINASEcoord: 231..249
score: 44.37
coord: 297..319
score: 29.91
coord: 193..206
score: 40.72
coord: 341..363
score: 37.55
IPR001245Serine-threonine/tyrosine-protein kinase, catalytic domainPFAMPF07714PK_Tyr_Ser-Thrcoord: 100..370
e-value: 1.4E-56
score: 191.7
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 92..373
e-value: 7.5E-55
score: 198.2
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 92..373
score: 40.399933
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 80..172
e-value: 1.6E-17
score: 65.5
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 196..386
e-value: 8.9E-61
score: 207.1
NoneNo IPR availablePIRSRPIRSR000606-51PIRSR000606-51coord: 191..317
e-value: 6.2E-24
score: 82.1
NoneNo IPR availablePANTHERPTHR44329:SF98KINASE ATMRK1, PUTATIVE-RELATEDcoord: 27..391
NoneNo IPR availablePANTHERPTHR44329SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATEDcoord: 27..391
NoneNo IPR availableCDDcd13999STKc_MAP3K-likecoord: 99..370
e-value: 4.97411E-111
score: 323.335
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 237..249
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 74..370

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI07G16060.1CSPI07G16060.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004672 protein kinase activity