CSPI07G15670 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI07G15670
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Descriptionpalmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like
LocationChr7: 14283740 .. 14286506 (-)
RNA-Seq ExpressionCSPI07G15670
SyntenyCSPI07G15670
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCACCACCAGCTATACTATACGAAAGAAAGAATAAGAGGAAAGAAATGGATGAAGTATCCAAAGAAAAGCCAAAGGAGATGGTGATGAAATCTCCTCCGAGGCTCTTTAGGAGAAAAAAATGGACAAAGTTTGATAGAAACGTTGCCTTTTACTTTCTCAGTTTGCATCTACTTTGTATTTTGGCACCCTTCTATTTTAGTTGGACTTGTTTGTTGCTCGCCTTAGGCTTATCTTATTTAACAGGTATGGGAATAAGTGTCTCGTATCATCGAAATCTTGCACATCGAAGCTTCAAGCTTCCAAAATGGCTCGAGTATTCTCTTGCTTATATTGCAACTCATGCAATCCAGGTTTGATATTTTGAATTCTTTGATTTTATATTTAGTTTAAGACTATACATGATGGATAATACAAAATATTTAAATTTTTTGTCACACATTAGTATAAACTTATAAATCAATAAGATATGTAACATAATATATATGGGAGTAGGTGGTTCATTATTGATTTTTACATATGGTAACCCTACAAGTGAGGATTTATTAGTGTTGCCTTAGATACTTTGGATTCATTTGTCATGCCAAAATCATCATTATTTATGGTTATGCCATATTTTGTGATAATTTGTATATACACTACTTAAAACACCAAAACATTATTAAAGAATGGTGTAGTGTAATTATTGCCTACATGTGATTTAGAAGGGTAAAACTAGTGACAAAACCCTAGTGCATATTTTTTTATATCTACCGTGTTTTGTTAAAATTTCGTTTACTTTTTTCTTTCTTAATTTTAGGGTGATCCAATTGATTGGGTGAGTACACATAGATGTCATCATCGATTCGTTGATTCAGCACGAGATCCACATAGTCCTATTTATGGATTCTGGTTTAGTCAAATGACTTGGTTTTTTGATTCGTATAACTTGACGAGAAAAGTTTGTCCAAACTATTTTAATAATTATCAAAAGGTGGAGAGAAACATTGCCATGTTTTACATGAAACATGGATATCCAGACAATGTGACAGATTTGGAGAAACAAAGGTTCTATAGATTTCTACATAAAACATATTGGCTTCATCCACTTGCCCTTTCACTCTTGCTCTATGCCATTGGAGGACTACCTTTCATCATTTGGGGAATGGTATAATTATTCCCATTCTTTTTTAGCTTTTACTCCTACAATTTGTAAGTTGTGATTAAATTTATTTATGAGTCCTCTAAAAATTAGTTTACATTCATCTTTTATAAATTCTATTTGTTTGGATTTTTGACATTTTAACTTAACTTTGGTAAACTAGCAAATTTAATAATTCTTTTTGAAGGAAAGATTGTATGTTTTTGTATTGACATAACAAAGGTAGTTAATTTTCTTTTTGACTATTTTACTTAAATGTTTACTCAAATATTATTGTTTATCTTTTATGAGCTGTGACAGACCAAAATAAACTATTATTTAAGTTCTATTGTGATAGACACATGTAATAGTCTATATTTTGATCCGTCATACCTAAATTGTGCTATTTTAATATATTTGTAGTAGTTTTGTCTTTTTTATTATTGTTTTATTTTACAATTAATCATTGATTAACATTATTTATTTGGTTAAACTATAAAATTTAATTAGTCTTCAACGTTTGAAACTTGTTCATGTCTAATTAAGTTTAAGTCTTTATAAAAGATCTAGAAGTTAAAAGTTATACTGTGTATTTAGTTAAATTCAATCTTTTATTTAAGAAGTTTGTGAATTTTAGGCTTCTTTGATAATAATTAATTTGTTATTTTTTTCTTCTCTTTTTTGTGTAAAACTTATATATACTTTTTTTCTCCCCATTTTTAATCTATGGTTTCAAATTGTTTGCAAATAATAATATTTTTAGTCAATTCCAATCAACAACCAAAATTTGTAAGATATATGTATTTTTTTATTTTACTTTTCAAAACTTGATCACTGTTTAATATGATTAGCCATAAATATAAGTAGAAAAGGTAGTTGAAATGTATTTTCTAAATGATGTAAGTTCCTTTTCTTCATAAATAGTTTTACCATCTAGTTAATTATTCTAGATTTAATGATTATAAAATAATAATTATAATTTTATTAATTATTTTCATTGAAATATTTTACTAAACTAAGTGTTTTGTGTTTATTAAACTTGCGTATATAGTGCGTGAGGTTCGTATACAGCTTGCATGTGACATCTATGGTCAATTCAGTATGTCATATATGGGGAAATCAACCATGGAATACTGGTGATTTATCCAAGAATAATTGGTATGACATTTATTTTATTTTACTTTCCTTTGAATCCCTCAAAATTCAATTTTACTCCCTACATTTTCAAAATACTTTTCACAACCCTAATTTAGATAAAATCATATTTGAATAATATATGTTCTCTTTTCGTTGTTCTTTTCTTCTTTTATAGGATGGTAGCTTTATTAACCTTTGGAGAAGGATGGCACAATAACCACCATGCTTTTGAGTATTCAGCGAGACATGGTCATGAGTGGTGGCAAATTGATTATGGTTGGTATCTTATTAGGTTCCTTCAGGTCATTGGATTAGCAACCAATGTGAAATTACCTTCTGAAAAGCAAAAATTGGCTGCTTTAAACAAATCAAAACCCATCTTTCTTAAGCTTATGATTGATTGAGATTAATCATATTTAATCTTTATATATATATATATATGCATGAATGCTTATGTACTCTATCATGGTAGGGTTTACGTAACCCTTCTCTCAATTATATGTATCCTTAC

mRNA sequence

CTCACCACCAGCTATACTATACGAAAGAAAGAATAAGAGGAAAGAAATGGATGAAGTATCCAAAGAAAAGCCAAAGGAGATGGTGATGAAATCTCCTCCGAGGCTCTTTAGGAGAAAAAAATGGACAAAGTTTGATAGAAACGTTGCCTTTTACTTTCTCAGTTTGCATCTACTTTGTATTTTGGCACCCTTCTATTTTAGTTGGACTTGTTTGTTGCTCGCCTTAGGCTTATCTTATTTAACAGGTATGGGAATAAGTGTCTCGTATCATCGAAATCTTGCACATCGAAGCTTCAAGCTTCCAAAATGGCTCGAGTATTCTCTTGCTTATATTGCAACTCATGCAATCCAGGGTGATCCAATTGATTGGGTGAGTACACATAGATGTCATCATCGATTCGTTGATTCAGCACGAGATCCACATAGTCCTATTTATGGATTCTGGTTTAGTCAAATGACTTGGTTTTTTGATTCGTATAACTTGACGAGAAAAGTTTGTCCAAACTATTTTAATAATTATCAAAAGGTGGAGAGAAACATTGCCATGTTTTACATGAAACATGGATATCCAGACAATGTGACAGATTTGGAGAAACAAAGGTTCTATAGATTTCTACATAAAACATATTGGCTTCATCCACTTGCCCTTTCACTCTTGCTCTATGCCATTGGAGGACTACCTTTCATCATTTGGGGAATGTGCGTGAGGTTCGTATACAGCTTGCATGTGACATCTATGGTCAATTCAGTATGTCATATATGGGGAAATCAACCATGGAATACTGGTGATTTATCCAAGAATAATTGGATGGTAGCTTTATTAACCTTTGGAGAAGGATGGCACAATAACCACCATGCTTTTGAGTATTCAGCGAGACATGGTCATGAGTGGTGGCAAATTGATTATGGTTGGTATCTTATTAGGTTCCTTCAGGTCATTGGATTAGCAACCAATGTGAAATTACCTTCTGAAAAGCAAAAATTGGCTGCTTTAAACAAATCAAAACCCATCTTTCTTAAGCTTATGATTGATTGAGATTAATCATATTTAATCTTTATATATATATATATATGCATGAATGCTTATGTACTCTATCATGGTAGGGTTTACGTAACCCTTCTCTCAATTATATGTATCCTTAC

Coding sequence (CDS)

ATGGATGAAGTATCCAAAGAAAAGCCAAAGGAGATGGTGATGAAATCTCCTCCGAGGCTCTTTAGGAGAAAAAAATGGACAAAGTTTGATAGAAACGTTGCCTTTTACTTTCTCAGTTTGCATCTACTTTGTATTTTGGCACCCTTCTATTTTAGTTGGACTTGTTTGTTGCTCGCCTTAGGCTTATCTTATTTAACAGGTATGGGAATAAGTGTCTCGTATCATCGAAATCTTGCACATCGAAGCTTCAAGCTTCCAAAATGGCTCGAGTATTCTCTTGCTTATATTGCAACTCATGCAATCCAGGGTGATCCAATTGATTGGGTGAGTACACATAGATGTCATCATCGATTCGTTGATTCAGCACGAGATCCACATAGTCCTATTTATGGATTCTGGTTTAGTCAAATGACTTGGTTTTTTGATTCGTATAACTTGACGAGAAAAGTTTGTCCAAACTATTTTAATAATTATCAAAAGGTGGAGAGAAACATTGCCATGTTTTACATGAAACATGGATATCCAGACAATGTGACAGATTTGGAGAAACAAAGGTTCTATAGATTTCTACATAAAACATATTGGCTTCATCCACTTGCCCTTTCACTCTTGCTCTATGCCATTGGAGGACTACCTTTCATCATTTGGGGAATGTGCGTGAGGTTCGTATACAGCTTGCATGTGACATCTATGGTCAATTCAGTATGTCATATATGGGGAAATCAACCATGGAATACTGGTGATTTATCCAAGAATAATTGGATGGTAGCTTTATTAACCTTTGGAGAAGGATGGCACAATAACCACCATGCTTTTGAGTATTCAGCGAGACATGGTCATGAGTGGTGGCAAATTGATTATGGTTGGTATCTTATTAGGTTCCTTCAGGTCATTGGATTAGCAACCAATGTGAAATTACCTTCTGAAAAGCAAAAATTGGCTGCTTTAAACAAATCAAAACCCATCTTTCTTAAGCTTATGATTGATTGA

Protein sequence

MDEVSKEKPKEMVMKSPPRLFRRKKWTKFDRNVAFYFLSLHLLCILAPFYFSWTCLLLALGLSYLTGMGISVSYHRNLAHRSFKLPKWLEYSLAYIATHAIQGDPIDWVSTHRCHHRFVDSARDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIAMFYMKHGYPDNVTDLEKQRFYRFLHKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVYSLHVTSMVNSVCHIWGNQPWNTGDLSKNNWMVALLTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYLIRFLQVIGLATNVKLPSEKQKLAALNKSKPIFLKLMID*
Homology
BLAST of CSPI07G15670 vs. ExPASy Swiss-Prot
Match: Q949X0 (Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ADS3 PE=1 SV=2)

HSP 1 Score: 371.7 bits (953), Expect = 8.6e-102
Identity = 173/302 (57.28%), Postives = 214/302 (70.86%), Query Frame = 0

Query: 12  MVMKSPPRLFRRKKWTKFDRNVAFYFLSLHLLCILAPFYFSWTCLLLALGLSYLTG-MGI 71
           +V K    ++  ++W   D       LS+HLL +LAPF F+W  + +A GL  +TG +GI
Sbjct: 84  LVKKKEKVVWWEREWKAMDFGAVAVVLSMHLLSLLAPFQFNWRAVSVAFGLYIVTGLLGI 143

Query: 72  SVSYHRNLAHRSFKLPKWLEYSLAYIATHAIQGDPIDWVSTHRCHHRFVDSARDPHSPIY 131
           ++S+HRNL+H++FKLPKWLEY  AY    A+QG+PIDWVSTHR HH+F DS RDPHSP+ 
Sbjct: 144 TLSFHRNLSHKAFKLPKWLEYLFAYCGAQALQGNPIDWVSTHRYHHQFCDSDRDPHSPLD 203

Query: 132 GFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIAMFYMKHGYPDNVTDLEKQRFYRFL 191
           GFWFS M W FD+  +T++                       G P+NV DLEKQ FYRFL
Sbjct: 204 GFWFSHMNWMFDTNTITQRC----------------------GEPNNVGDLEKQPFYRFL 263

Query: 192 HKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVYSLHVTSMVNSVCHIWGNQPWNTGDLS 251
             TY LHPLAL++ LYA+GG PFI+WGM VR V+  H+T +VNS CH+WG Q WNTGDLS
Sbjct: 264 RTTYILHPLALAVALYAMGGFPFIVWGMGVRIVWVYHITWLVNSACHVWGKQAWNTGDLS 323

Query: 252 KNNWMVALLTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYLIRFLQVIGLATNVKLPSEK 311
           KNNW VA L FGEGWHNNHHAFE+SARHG EWWQ+D  WY+++FLQ IGLAT+VKLPSE 
Sbjct: 324 KNNWWVAALAFGEGWHNNHHAFEFSARHGLEWWQLDMTWYVVKFLQAIGLATDVKLPSEA 363

Query: 312 QK 313
           QK
Sbjct: 384 QK 363

BLAST of CSPI07G15670 vs. ExPASy Swiss-Prot
Match: Q9LVZ3 (Probable lipid desaturase ADS3.2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ADS3.2 PE=2 SV=3)

HSP 1 Score: 333.6 bits (854), Expect = 2.6e-90
Identity = 157/301 (52.16%), Postives = 195/301 (64.78%), Query Frame = 0

Query: 11  EMVMKSPPRLFRRKKWTKFDRNVAFYFLSLHLLCILAPFYFSWTCLLLALGLSYLTGMGI 70
           E+V+    R F  + WT +D +    F+  HLL +LAPFYFSW    +   L ++ G+ I
Sbjct: 76  EVVVVRTKRSFWERSWTSWDVSKLVIFVGTHLLSLLAPFYFSWEAFWVFPWLVFINGICI 135

Query: 71  SVSYHRNLAHRSFKLPKWLEYSLAYIATHAIQGDPIDWVSTHRCHHRFVDSARDPHSPIY 130
           ++SYHRNL+HRSF LPKWLEY  AY    A QGDPI+WVS HR HH+  ++ RDPHSP  
Sbjct: 136 TLSYHRNLSHRSFDLPKWLEYLFAYGGVLAFQGDPIEWVSNHRYHHKHCETQRDPHSPTQ 195

Query: 131 GFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIAMFYMKHGYPDNVTDLEKQRFYRFL 190
           GFWFS M W FD+ ++                          G  +NV DL +Q FYRFL
Sbjct: 196 GFWFSHMAWIFDTSSILENC----------------------GGEENVDDLVRQPFYRFL 255

Query: 191 HKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVYSLHVTSMVNSVCHIWGNQPWNTGDLS 250
            +T  LH +A S L Y  GG+P ++WG+ +     LH+T +VNSVCHIWG + WNT D S
Sbjct: 256 QRTVLLHMMAYSFLFYFCGGMPLLVWGIGITIAVRLHLTFLVNSVCHIWGTRAWNTSDFS 315

Query: 251 KNNWMVALLTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYLIRFLQVIGLATNVKLPSEK 310
           KNNW VA+LT GEGWHNNHHAFE+SARHG EWWQ+D  W LIRFL+ IGLATNVKLP+E 
Sbjct: 316 KNNWWVAILTLGEGWHNNHHAFEFSARHGLEWWQLDITWCLIRFLEAIGLATNVKLPTET 354

Query: 311 Q 312
           Q
Sbjct: 376 Q 354

BLAST of CSPI07G15670 vs. ExPASy Swiss-Prot
Match: O65797 (Delta-9 acyl-lipid desaturase 1 OS=Arabidopsis thaliana OX=3702 GN=ADS1 PE=2 SV=1)

HSP 1 Score: 325.5 bits (833), Expect = 7.1e-88
Identity = 154/294 (52.38%), Postives = 192/294 (65.31%), Query Frame = 0

Query: 19  RLFRRKKWTKFDRNVAFYFLSLHLLCILAPFYFSWTCLLLALGLSYLTGMGISVSYHRNL 78
           R    +KW + D   AF  L +H LC+LAPF F+W  L +AL +  + G+GI+VSYHRNL
Sbjct: 27  RAMWERKWKRLDIVKAFASLFVHFLCLLAPFNFTWPALRVALIVYTVGGLGITVSYHRNL 86

Query: 79  AHRSFKLPKWLEYSLAYIATHAIQGDPIDWVSTHRCHHRFVDSARDPHSPIYGFWFSQMT 138
           AHRSFK+PKWLEY  AY    AIQGDPIDWVSTHR HH+F DS RDPHSP  GFWFS + 
Sbjct: 87  AHRSFKVPKWLEYFFAYCGLLAIQGDPIDWVSTHRYHHQFTDSDRDPHSPNEGFWFSHLL 146

Query: 139 WFFDSYNLTRKVCPNYFNNYQKVERNIAMFYMKHGYPDNVTDLEKQRFYRFLHKTYWLHP 198
           W FD+  L  K                       G   NV DL++Q +Y+FL +T   H 
Sbjct: 147 WLFDTGYLVEKC----------------------GRRTNVEDLKRQWYYKFLQRTVLYHI 206

Query: 199 LALSLLLYAIGGLPFIIWGMCVRFVYSLHVTSMVNSVCHIWGNQPWNTGDLSKNNWMVAL 258
           L    LLY  GGL F+ WGM +      HVT ++NS+CH+WG++ W T D S+N W +++
Sbjct: 207 LTFGFLLYYFGGLSFLTWGMGIGVAMEHHVTCLINSLCHVWGSRTWKTNDTSRNVWWLSV 266

Query: 259 LTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYLIRFLQVIGLATNVKLPSEKQK 313
            +FGE WHNNHHAFE SAR G EWWQID  WY++RFL++IGLAT+VKLPSE Q+
Sbjct: 267 FSFGESWHNNHHAFESSARQGLEWWQIDISWYIVRFLEIIGLATDVKLPSESQR 298

BLAST of CSPI07G15670 vs. ExPASy Swiss-Prot
Match: P0DOW2 (Acyl-CoA C20 Delta5-desaturase OS=Anemone leveillei OX=212809 GN=AL10 PE=1 SV=1)

HSP 1 Score: 322.0 bits (824), Expect = 7.8e-87
Identity = 152/297 (51.18%), Postives = 194/297 (65.32%), Query Frame = 0

Query: 24  KKWTKFDRNVAFYFLSLHLLCILAPFYFSWTCLLLALGLSYLTGM-GISVSYHRNLAHRS 83
           +KW   D     +   LH+LC+ AP  FSW    +   L  + G+ G ++S+HRNL HRS
Sbjct: 38  RKWNSTDVFHILFIGGLHVLCLFAPSTFSWKSFWVCFALYAICGVFGTTLSFHRNLTHRS 97

Query: 84  FKLPKWLEYSLAYIATHAIQGDPIDWVSTHRCHHRFVDSARDPHSPIYGFWFSQMTWFFD 143
           FKLPK+LEY  AY+  HA+QGDP+ WVSTHR HH++ D+  DPHSPI GFWF  + W FD
Sbjct: 98  FKLPKYLEYFFAYVGLHALQGDPVWWVSTHRYHHKYTDTYLDPHSPIEGFWFCHIFWLFD 157

Query: 144 SYNLTRKVCPNYFNNYQKVERNIAMFYMKHGYPDNVTDLEKQRFYRFLHKTYWLHPLALS 203
           S  +  K C  Y                     +N  DL KQ +YRFL +T+  H    +
Sbjct: 158 SKYIIEK-CGRY---------------------ENAGDLMKQSYYRFLERTFVYHVYLQA 217

Query: 204 LLLYAIGGLPFIIWGMCVRFVYSLHVTSMVNSVCHIWGNQPWNTGDLSKNNWMVALLTFG 263
            LLY  GG PFI+WGM VR +   H++ +VNSVCH WGN+PWNTGDLS NNW +A+LT G
Sbjct: 218 ALLYLFGGFPFIVWGMAVRTILGFHLSWLVNSVCHRWGNRPWNTGDLSTNNWFIAMLTSG 277

Query: 264 EGWHNNHHAFEYSARHGHEWWQIDYGWYLIRFLQVIGLATNVKLPSE--KQKLAALN 318
           EGWHNNHHAFEYSARHG EWWQID  WY+I+ L+ +GLAT++K+PSE  K+K++  N
Sbjct: 278 EGWHNNHHAFEYSARHGIEWWQIDTTWYIIKLLEYLGLATDIKVPSEIHKRKMSFKN 312

BLAST of CSPI07G15670 vs. ExPASy Swiss-Prot
Match: P0DOW3 (Acyl-CoA 5-desaturase AL21 OS=Anemone leveillei OX=212809 GN=AL21 PE=1 SV=1)

HSP 1 Score: 319.3 bits (817), Expect = 5.1e-86
Identity = 151/274 (55.11%), Postives = 188/274 (68.61%), Query Frame = 0

Query: 40  LHLLCILAPFYFSWTCLLLALGLSYLTGM-GISVSYHRNLAHRSFKLPKWLEYSLAYIAT 99
           LH+LC+ APF FSW+   L+L L  + G+ G ++SYHRNL HRSFKLPK+LEY  AY+  
Sbjct: 51  LHVLCLSAPFTFSWSAFWLSLTLYAVCGVFGTTLSYHRNLTHRSFKLPKYLEYFFAYVGL 110

Query: 100 HAIQGDPIDWVSTHRCHHRFVDSARDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNY 159
           HA+QGDP+ WVSTHR HH+F D+  DPHSPI GFWFS + W FDS  +  + C  Y    
Sbjct: 111 HALQGDPVWWVSTHRYHHKFTDTYLDPHSPIEGFWFSHIFWLFDSKYILEE-CGRY---- 170

Query: 160 QKVERNIAMFYMKHGYPDNVTDLEKQRFYRFLHKTYWLHPLALSLLLYAIGGLPFIIWGM 219
                            +N  DL KQ +YRFL +T+  H    + LLY  GG PFI+WGM
Sbjct: 171 -----------------ENAGDLLKQSYYRFLERTFVFHVYLQAALLYMFGGFPFIVWGM 230

Query: 220 CVRFVYSLHVTSMVNSVCHIWGNQPWNTGDLSKNNWMVALLTFGEGWHNNHHAFEYSARH 279
            VR V+  H + +VNSVCH +G+Q W+TGDLS NNW +A+LT GEGWHNNHHAFEYSARH
Sbjct: 231 AVRTVWGFHASWLVNSVCHRYGHQAWDTGDLSTNNWFIAMLTSGEGWHNNHHAFEYSARH 290

Query: 280 GHEWWQIDYGWYLIRFLQVIGLATNVKLPSEKQK 313
           G EWWQID  WY+I+ L+ +GLAT+VK+PSE  K
Sbjct: 291 GIEWWQIDTTWYVIKLLEYLGLATDVKVPSEVHK 302

BLAST of CSPI07G15670 vs. ExPASy TrEMBL
Match: A0A0A0KAB1 (FA_desaturase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G376370 PE=3 SV=1)

HSP 1 Score: 698.7 bits (1802), Expect = 1.1e-197
Identity = 326/329 (99.09%), Postives = 328/329 (99.70%), Query Frame = 0

Query: 1   MDEVSKEKPKEMVMKSPPRLFRRKKWTKFDRNVAFYFLSLHLLCILAPFYFSWTCLLLAL 60
           MDEVSKEKPKEMVMKSPPRLFRRKKWTKFDRNVAFYFLSLHLLCILAPFYFSWTCLLLAL
Sbjct: 1   MDEVSKEKPKEMVMKSPPRLFRRKKWTKFDRNVAFYFLSLHLLCILAPFYFSWTCLLLAL 60

Query: 61  GLSYLTGMGISVSYHRNLAHRSFKLPKWLEYSLAYIATHAIQGDPIDWVSTHRCHHRFVD 120
           GLSYLTGMGISVSYHRNLAHRSFKLPKWLEYSLAYIATHAIQGDPIDWVSTHRCHHRFVD
Sbjct: 61  GLSYLTGMGISVSYHRNLAHRSFKLPKWLEYSLAYIATHAIQGDPIDWVSTHRCHHRFVD 120

Query: 121 SARDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIAMFYMKHGYPDNVTD 180
           SARDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNI MFYMKHGYPDNVTD
Sbjct: 121 SARDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIFMFYMKHGYPDNVTD 180

Query: 181 LEKQRFYRFLHKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVYSLHVTSMVNSVCHIWG 240
           LEKQ+FYRF+HKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVYSLHVTSMVNSVCHIWG
Sbjct: 181 LEKQKFYRFIHKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVYSLHVTSMVNSVCHIWG 240

Query: 241 NQPWNTGDLSKNNWMVALLTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYLIRFLQVIGL 300
           NQPWNTGDLSKNNWMVALLTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYLIRFLQVIGL
Sbjct: 241 NQPWNTGDLSKNNWMVALLTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYLIRFLQVIGL 300

Query: 301 ATNVKLPSEKQKLAALNKSKPIFLKLMID 330
           ATNVKLPSEKQKLAALNKSKPIFLKLMID
Sbjct: 301 ATNVKLPSEKQKLAALNKSKPIFLKLMID 329

BLAST of CSPI07G15670 vs. ExPASy TrEMBL
Match: A0A1S3BS49 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103492608 PE=3 SV=1)

HSP 1 Score: 645.2 bits (1663), Expect = 1.5e-181
Identity = 297/329 (90.27%), Postives = 310/329 (94.22%), Query Frame = 0

Query: 1   MDEVSKEKPKEMVMKSPPRLFRRKKWTKFDRNVAFYFLSLHLLCILAPFYFSWTCLLLAL 60
           M+E SKE+ KEMVM SP RLF+RKKWTKFD+NVAFYFL LHLLCILAPFYFSWTCL L+L
Sbjct: 1   MEEASKEESKEMVMNSPSRLFKRKKWTKFDKNVAFYFLLLHLLCILAPFYFSWTCLFLSL 60

Query: 61  GLSYLTGMGISVSYHRNLAHRSFKLPKWLEYSLAYIATHAIQGDPIDWVSTHRCHHRFVD 120
           GL+ LTGMGISVSYHRNLAHRSFKLPKWLEYSLAYIA HAIQGDPIDWVSTHRCHHRFVD
Sbjct: 61  GLANLTGMGISVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVD 120

Query: 121 SARDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIAMFYMKHGYPDNVTD 180
           S RDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNN+QKVERN  MFYMKHGY DNVTD
Sbjct: 121 SERDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNFQKVERNAFMFYMKHGYADNVTD 180

Query: 181 LEKQRFYRFLHKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVYSLHVTSMVNSVCHIWG 240
           LEKQRFYRF+HKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFV SLH TSM+NSVCHIWG
Sbjct: 181 LEKQRFYRFIHKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVISLHFTSMINSVCHIWG 240

Query: 241 NQPWNTGDLSKNNWMVALLTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYLIRFLQVIGL 300
           NQPWNTGDLSKNNW+VA+ TFGEGWHNNHHAFEYSARHGHEWWQIDYGWY+I+FLQVIG 
Sbjct: 241 NQPWNTGDLSKNNWIVAIFTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYIIKFLQVIGF 300

Query: 301 ATNVKLPSEKQKLAALNKSKPIFLKLMID 330
           ATNVKLPSEKQK AALNKSKP FLK MID
Sbjct: 301 ATNVKLPSEKQKWAALNKSKPTFLKPMID 329

BLAST of CSPI07G15670 vs. ExPASy TrEMBL
Match: A0A1S3BQG5 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103492609 PE=3 SV=1)

HSP 1 Score: 634.0 bits (1634), Expect = 3.4e-178
Identity = 297/329 (90.27%), Postives = 308/329 (93.62%), Query Frame = 0

Query: 2   DEVSKEKPKEMV-MKSPPRLFRRKKWTKFDRNVAFYFLSLHLLCILAPFYFSWTCLLLAL 61
           +EVSKEKPKE++ MKSPP+LFRRKKWTKFDRN AFYFL+ HLLCILAPF FSWT LLLAL
Sbjct: 3   NEVSKEKPKEIILMKSPPKLFRRKKWTKFDRNFAFYFLTFHLLCILAPFCFSWTRLLLAL 62

Query: 62  GLSYLTGMGISVSYHRNLAHRSFKLPKWLEYSLAYIATHAIQGDPIDWVSTHRCHHRFVD 121
           GLS L+GMGISVSYHRNLAHRSFKLPKW EYSLAYIA HAIQGDPIDWVSTHRCHHRFVD
Sbjct: 63  GLSCLSGMGISVSYHRNLAHRSFKLPKWFEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVD 122

Query: 122 SARDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIAMFYMKHGYPDNVTD 181
           S RDPHSPIYGFWFSQMTW FDSYNLTRKVCPNYFNNYQK ERNI M YMKHGYPDNV D
Sbjct: 123 SERDPHSPIYGFWFSQMTWLFDSYNLTRKVCPNYFNNYQKAERNIFMLYMKHGYPDNVPD 182

Query: 182 LEKQRFYRFLHKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVYSLHVTSMVNSVCHIWG 241
           LEKQ FYRF+HKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVYS+HVTSMVNSVCH+WG
Sbjct: 183 LEKQWFYRFIHKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVYSVHVTSMVNSVCHMWG 242

Query: 242 NQPWNTGDLSKNNWMVALLTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYLIRFLQVIGL 301
           NQPWNTGDLSKNNWMVALLTFGEGWHNNHHAFEYSARHG EWWQIDYGWY IRFL+VIGL
Sbjct: 243 NQPWNTGDLSKNNWMVALLTFGEGWHNNHHAFEYSARHGLEWWQIDYGWYFIRFLEVIGL 302

Query: 302 ATNVKLPSEKQKLAALNKSKPIFLKLMID 330
           ATNVK PSEKQKLAALNKSKP FLK  I+
Sbjct: 303 ATNVKFPSEKQKLAALNKSKPTFLKPTIN 331

BLAST of CSPI07G15670 vs. ExPASy TrEMBL
Match: A0A6J1JUL6 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111488510 PE=3 SV=1)

HSP 1 Score: 582.4 bits (1500), Expect = 1.2e-162
Identity = 265/319 (83.07%), Postives = 289/319 (90.60%), Query Frame = 0

Query: 3   EVSKEKPKEMVMKSPPRLFRRKKWTKFDRNVAFYFLSLHLLCILAPFYFSWTCLLLALGL 62
           E SK+ P   +++SPP LF RKKWTKFDRNVA YFL LHLLCILAPFYF+WTC  L + L
Sbjct: 2   EASKQHPN--MVQSPPMLFTRKKWTKFDRNVASYFLLLHLLCILAPFYFTWTCFFLTIIL 61

Query: 63  SYLTGMGISVSYHRNLAHRSFKLPKWLEYSLAYIATHAIQGDPIDWVSTHRCHHRFVDSA 122
           + LTGM ISVSYHRNLAHR+FKLPKWLEYSLAY A HAIQGDPIDWVSTHRCHHR+VD+ 
Sbjct: 62  ANLTGMAISVSYHRNLAHRAFKLPKWLEYSLAYCAAHAIQGDPIDWVSTHRCHHRYVDTE 121

Query: 123 RDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIAMFYMKHGYPDNVTDLE 182
           RDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYF N++KVERN+ MF+MKHGYPDNV DLE
Sbjct: 122 RDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFKNFEKVERNMFMFFMKHGYPDNVRDLE 181

Query: 183 KQRFYRFLHKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVYSLHVTSMVNSVCHIWGNQ 242
           KQ FYRF+ KTYWLHPLAL+ LLYA GG+P++IWGMCVRFV SLHVTSMVNSVCHIWGNQ
Sbjct: 182 KQWFYRFMRKTYWLHPLALAGLLYAAGGIPYVIWGMCVRFVLSLHVTSMVNSVCHIWGNQ 241

Query: 243 PWNTGDLSKNNWMVALLTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYLIRFLQVIGLAT 302
           PWNTGDLS+NNWMVAL+TFGEGWHNNHHAFEYSARHGHEWWQIDYGWY+I+FLQVIGLAT
Sbjct: 242 PWNTGDLSRNNWMVALITFGEGWHNNHHAFEYSARHGHEWWQIDYGWYIIKFLQVIGLAT 301

Query: 303 NVKLPSEKQKLAALNKSKP 322
            VKLPS+   LAALNKSKP
Sbjct: 302 CVKLPSQTHNLAALNKSKP 318

BLAST of CSPI07G15670 vs. ExPASy TrEMBL
Match: A0A6J1GPG5 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111456275 PE=3 SV=1)

HSP 1 Score: 579.3 bits (1492), Expect = 1.0e-161
Identity = 263/319 (82.45%), Postives = 290/319 (90.91%), Query Frame = 0

Query: 3   EVSKEKPKEMVMKSPPRLFRRKKWTKFDRNVAFYFLSLHLLCILAPFYFSWTCLLLALGL 62
           E SK+ P   +++SPP LF RKKWTKFDRNVA YFL LHLLCILAPFYF+WT   L + L
Sbjct: 2   EASKQHPN--MVQSPPMLFTRKKWTKFDRNVASYFLLLHLLCILAPFYFTWTSFFLTIIL 61

Query: 63  SYLTGMGISVSYHRNLAHRSFKLPKWLEYSLAYIATHAIQGDPIDWVSTHRCHHRFVDSA 122
           + LTGM ISVSYHRNLAHR+FKLPKWLEYSLAY A HAIQGDPIDWVSTHRCHHR+VD+ 
Sbjct: 62  ANLTGMAISVSYHRNLAHRAFKLPKWLEYSLAYCAAHAIQGDPIDWVSTHRCHHRYVDTE 121

Query: 123 RDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIAMFYMKHGYPDNVTDLE 182
           RDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYF N++KVERN+ MF+MKHGYPDNV DLE
Sbjct: 122 RDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFKNFEKVERNMFMFFMKHGYPDNVRDLE 181

Query: 183 KQRFYRFLHKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVYSLHVTSMVNSVCHIWGNQ 242
           KQ FYRF+ KTYWLHPLAL+ LLYA+GG+P++IWGMCVRFV SLH+TSMVNSVCHIWG Q
Sbjct: 182 KQWFYRFMRKTYWLHPLALAGLLYAVGGVPYVIWGMCVRFVLSLHITSMVNSVCHIWGTQ 241

Query: 243 PWNTGDLSKNNWMVALLTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYLIRFLQVIGLAT 302
           PWNTGDLS+NNWMVAL+TFGEGWHNNHHAFEYSARHGHEWW+IDYGWY+I+FLQVIGLAT
Sbjct: 242 PWNTGDLSRNNWMVALITFGEGWHNNHHAFEYSARHGHEWWEIDYGWYVIKFLQVIGLAT 301

Query: 303 NVKLPSEKQKLAALNKSKP 322
            VKLPS+ QKLAALNKSKP
Sbjct: 302 CVKLPSQTQKLAALNKSKP 318

BLAST of CSPI07G15670 vs. NCBI nr
Match: XP_011659261.2 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic [Cucumis sativus] >KAE8646293.1 hypothetical protein Csa_016268 [Cucumis sativus])

HSP 1 Score: 703.0 bits (1813), Expect = 1.2e-198
Identity = 329/329 (100.00%), Postives = 329/329 (100.00%), Query Frame = 0

Query: 1   MDEVSKEKPKEMVMKSPPRLFRRKKWTKFDRNVAFYFLSLHLLCILAPFYFSWTCLLLAL 60
           MDEVSKEKPKEMVMKSPPRLFRRKKWTKFDRNVAFYFLSLHLLCILAPFYFSWTCLLLAL
Sbjct: 1   MDEVSKEKPKEMVMKSPPRLFRRKKWTKFDRNVAFYFLSLHLLCILAPFYFSWTCLLLAL 60

Query: 61  GLSYLTGMGISVSYHRNLAHRSFKLPKWLEYSLAYIATHAIQGDPIDWVSTHRCHHRFVD 120
           GLSYLTGMGISVSYHRNLAHRSFKLPKWLEYSLAYIATHAIQGDPIDWVSTHRCHHRFVD
Sbjct: 61  GLSYLTGMGISVSYHRNLAHRSFKLPKWLEYSLAYIATHAIQGDPIDWVSTHRCHHRFVD 120

Query: 121 SARDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIAMFYMKHGYPDNVTD 180
           SARDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIAMFYMKHGYPDNVTD
Sbjct: 121 SARDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIAMFYMKHGYPDNVTD 180

Query: 181 LEKQRFYRFLHKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVYSLHVTSMVNSVCHIWG 240
           LEKQRFYRFLHKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVYSLHVTSMVNSVCHIWG
Sbjct: 181 LEKQRFYRFLHKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVYSLHVTSMVNSVCHIWG 240

Query: 241 NQPWNTGDLSKNNWMVALLTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYLIRFLQVIGL 300
           NQPWNTGDLSKNNWMVALLTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYLIRFLQVIGL
Sbjct: 241 NQPWNTGDLSKNNWMVALLTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYLIRFLQVIGL 300

Query: 301 ATNVKLPSEKQKLAALNKSKPIFLKLMID 330
           ATNVKLPSEKQKLAALNKSKPIFLKLMID
Sbjct: 301 ATNVKLPSEKQKLAALNKSKPIFLKLMID 329

BLAST of CSPI07G15670 vs. NCBI nr
Match: XP_038899717.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 666.8 bits (1719), Expect = 9.9e-188
Identity = 307/329 (93.31%), Postives = 319/329 (96.96%), Query Frame = 0

Query: 1   MDEVSKEKPKEMVMKSPPRLFRRKKWTKFDRNVAFYFLSLHLLCILAPFYFSWTCLLLAL 60
           M+E SKE PKEMVMKSPPRLFRRKKWTKFDRNVAFYFL LHLLCILAPFYFSWTCL LAL
Sbjct: 1   MEEASKEGPKEMVMKSPPRLFRRKKWTKFDRNVAFYFLILHLLCILAPFYFSWTCLFLAL 60

Query: 61  GLSYLTGMGISVSYHRNLAHRSFKLPKWLEYSLAYIATHAIQGDPIDWVSTHRCHHRFVD 120
           GLSYLTGMGISVSYHRNLAHRSFKLPKWLEYSLAYIATHAIQGDPIDWVSTHRCHHR+VD
Sbjct: 61  GLSYLTGMGISVSYHRNLAHRSFKLPKWLEYSLAYIATHAIQGDPIDWVSTHRCHHRYVD 120

Query: 121 SARDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIAMFYMKHGYPDNVTD 180
           S RDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNN+QKVERN  MFYMKHG+PDNVTD
Sbjct: 121 SERDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNFQKVERNAFMFYMKHGHPDNVTD 180

Query: 181 LEKQRFYRFLHKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVYSLHVTSMVNSVCHIWG 240
           LEKQRFYRF+HKTYWLHPLALS+LLYAIGG+PFIIWGMCVRFV+SLHVTSMVNSVCHIWG
Sbjct: 181 LEKQRFYRFIHKTYWLHPLALSVLLYAIGGVPFIIWGMCVRFVFSLHVTSMVNSVCHIWG 240

Query: 241 NQPWNTGDLSKNNWMVALLTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYLIRFLQVIGL 300
            QPWNTGDLSKNNWMVA+LTFGEGWHNNHHAFEYSARHGHEWWQID+GWY+I+FLQVIGL
Sbjct: 241 KQPWNTGDLSKNNWMVAILTFGEGWHNNHHAFEYSARHGHEWWQIDFGWYIIKFLQVIGL 300

Query: 301 ATNVKLPSEKQKLAALNKSKPIFLKLMID 330
           ATNVKLPSEKQKLAALNKSKP FLK MID
Sbjct: 301 ATNVKLPSEKQKLAALNKSKPTFLKPMID 329

BLAST of CSPI07G15670 vs. NCBI nr
Match: XP_008451258.1 (PREDICTED: palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Cucumis melo])

HSP 1 Score: 645.2 bits (1663), Expect = 3.1e-181
Identity = 297/329 (90.27%), Postives = 310/329 (94.22%), Query Frame = 0

Query: 1   MDEVSKEKPKEMVMKSPPRLFRRKKWTKFDRNVAFYFLSLHLLCILAPFYFSWTCLLLAL 60
           M+E SKE+ KEMVM SP RLF+RKKWTKFD+NVAFYFL LHLLCILAPFYFSWTCL L+L
Sbjct: 1   MEEASKEESKEMVMNSPSRLFKRKKWTKFDKNVAFYFLLLHLLCILAPFYFSWTCLFLSL 60

Query: 61  GLSYLTGMGISVSYHRNLAHRSFKLPKWLEYSLAYIATHAIQGDPIDWVSTHRCHHRFVD 120
           GL+ LTGMGISVSYHRNLAHRSFKLPKWLEYSLAYIA HAIQGDPIDWVSTHRCHHRFVD
Sbjct: 61  GLANLTGMGISVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVD 120

Query: 121 SARDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIAMFYMKHGYPDNVTD 180
           S RDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNN+QKVERN  MFYMKHGY DNVTD
Sbjct: 121 SERDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNFQKVERNAFMFYMKHGYADNVTD 180

Query: 181 LEKQRFYRFLHKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVYSLHVTSMVNSVCHIWG 240
           LEKQRFYRF+HKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFV SLH TSM+NSVCHIWG
Sbjct: 181 LEKQRFYRFIHKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVISLHFTSMINSVCHIWG 240

Query: 241 NQPWNTGDLSKNNWMVALLTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYLIRFLQVIGL 300
           NQPWNTGDLSKNNW+VA+ TFGEGWHNNHHAFEYSARHGHEWWQIDYGWY+I+FLQVIG 
Sbjct: 241 NQPWNTGDLSKNNWIVAIFTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYIIKFLQVIGF 300

Query: 301 ATNVKLPSEKQKLAALNKSKPIFLKLMID 330
           ATNVKLPSEKQK AALNKSKP FLK MID
Sbjct: 301 ATNVKLPSEKQKWAALNKSKPTFLKPMID 329

BLAST of CSPI07G15670 vs. NCBI nr
Match: XP_011659770.1 (palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic [Cucumis sativus])

HSP 1 Score: 634.4 bits (1635), Expect = 5.4e-178
Identity = 294/330 (89.09%), Postives = 310/330 (93.94%), Query Frame = 0

Query: 1   MDEVSKEKPKEMVMKSPPRLFRRKKWTKFDRNVAFYFLSLHLLCILAP-FYFSWTCLLLA 60
           M+  SKE PKEMVM SP RLF+RKKWTKFD+NVAFYFL LHLL ILAP FYFSW CLLL+
Sbjct: 1   MEGASKEDPKEMVMSSPSRLFKRKKWTKFDKNVAFYFLLLHLLSILAPLFYFSWGCLLLS 60

Query: 61  LGLSYLTGMGISVSYHRNLAHRSFKLPKWLEYSLAYIATHAIQGDPIDWVSTHRCHHRFV 120
           LGL+ LTGM ISVSYHRNLAHRSFKLPKWLEYSLAYIA HAIQGDPIDWVSTHRCHHRFV
Sbjct: 61  LGLANLTGMAISVSYHRNLAHRSFKLPKWLEYSLAYIAAHAIQGDPIDWVSTHRCHHRFV 120

Query: 121 DSARDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIAMFYMKHGYPDNVT 180
           DS RDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNI MFYMKHGYPDNVT
Sbjct: 121 DSERDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIFMFYMKHGYPDNVT 180

Query: 181 DLEKQRFYRFLHKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVYSLHVTSMVNSVCHIW 240
           DLEKQ+FYRF+HKTYWLHPLALSLLLYA+GGLPFIIWG+CVRFV SLH TSM+NSVCHIW
Sbjct: 181 DLEKQKFYRFIHKTYWLHPLALSLLLYALGGLPFIIWGVCVRFVLSLHFTSMINSVCHIW 240

Query: 241 GNQPWNTGDLSKNNWMVALLTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYLIRFLQVIG 300
           GNQPWNTGDLSKNNW+VA+ TFGEGWHNNHHAFEYSARHGHEWWQID GWY+I+FLQVIG
Sbjct: 241 GNQPWNTGDLSKNNWIVAIFTFGEGWHNNHHAFEYSARHGHEWWQIDNGWYIIKFLQVIG 300

Query: 301 LATNVKLPSEKQKLAALNKSKPIFLKLMID 330
           LATNVKLPSEKQK+AALNKSKP FL+ MID
Sbjct: 301 LATNVKLPSEKQKMAALNKSKPTFLRPMID 330

BLAST of CSPI07G15670 vs. NCBI nr
Match: XP_008451259.1 (PREDICTED: palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [Cucumis melo])

HSP 1 Score: 634.0 bits (1634), Expect = 7.1e-178
Identity = 297/329 (90.27%), Postives = 308/329 (93.62%), Query Frame = 0

Query: 2   DEVSKEKPKEMV-MKSPPRLFRRKKWTKFDRNVAFYFLSLHLLCILAPFYFSWTCLLLAL 61
           +EVSKEKPKE++ MKSPP+LFRRKKWTKFDRN AFYFL+ HLLCILAPF FSWT LLLAL
Sbjct: 3   NEVSKEKPKEIILMKSPPKLFRRKKWTKFDRNFAFYFLTFHLLCILAPFCFSWTRLLLAL 62

Query: 62  GLSYLTGMGISVSYHRNLAHRSFKLPKWLEYSLAYIATHAIQGDPIDWVSTHRCHHRFVD 121
           GLS L+GMGISVSYHRNLAHRSFKLPKW EYSLAYIA HAIQGDPIDWVSTHRCHHRFVD
Sbjct: 63  GLSCLSGMGISVSYHRNLAHRSFKLPKWFEYSLAYIAAHAIQGDPIDWVSTHRCHHRFVD 122

Query: 122 SARDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIAMFYMKHGYPDNVTD 181
           S RDPHSPIYGFWFSQMTW FDSYNLTRKVCPNYFNNYQK ERNI M YMKHGYPDNV D
Sbjct: 123 SERDPHSPIYGFWFSQMTWLFDSYNLTRKVCPNYFNNYQKAERNIFMLYMKHGYPDNVPD 182

Query: 182 LEKQRFYRFLHKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVYSLHVTSMVNSVCHIWG 241
           LEKQ FYRF+HKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVYS+HVTSMVNSVCH+WG
Sbjct: 183 LEKQWFYRFIHKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVYSVHVTSMVNSVCHMWG 242

Query: 242 NQPWNTGDLSKNNWMVALLTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYLIRFLQVIGL 301
           NQPWNTGDLSKNNWMVALLTFGEGWHNNHHAFEYSARHG EWWQIDYGWY IRFL+VIGL
Sbjct: 243 NQPWNTGDLSKNNWMVALLTFGEGWHNNHHAFEYSARHGLEWWQIDYGWYFIRFLEVIGL 302

Query: 302 ATNVKLPSEKQKLAALNKSKPIFLKLMID 330
           ATNVK PSEKQKLAALNKSKP FLK  I+
Sbjct: 303 ATNVKFPSEKQKLAALNKSKPTFLKPTIN 331

BLAST of CSPI07G15670 vs. TAIR 10
Match: AT3G15850.1 (fatty acid desaturase 5 )

HSP 1 Score: 371.7 bits (953), Expect = 6.1e-103
Identity = 173/302 (57.28%), Postives = 214/302 (70.86%), Query Frame = 0

Query: 12  MVMKSPPRLFRRKKWTKFDRNVAFYFLSLHLLCILAPFYFSWTCLLLALGLSYLTG-MGI 71
           +V K    ++  ++W   D       LS+HLL +LAPF F+W  + +A GL  +TG +GI
Sbjct: 84  LVKKKEKVVWWEREWKAMDFGAVAVVLSMHLLSLLAPFQFNWRAVSVAFGLYIVTGLLGI 143

Query: 72  SVSYHRNLAHRSFKLPKWLEYSLAYIATHAIQGDPIDWVSTHRCHHRFVDSARDPHSPIY 131
           ++S+HRNL+H++FKLPKWLEY  AY    A+QG+PIDWVSTHR HH+F DS RDPHSP+ 
Sbjct: 144 TLSFHRNLSHKAFKLPKWLEYLFAYCGAQALQGNPIDWVSTHRYHHQFCDSDRDPHSPLD 203

Query: 132 GFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIAMFYMKHGYPDNVTDLEKQRFYRFL 191
           GFWFS M W FD+  +T++                       G P+NV DLEKQ FYRFL
Sbjct: 204 GFWFSHMNWMFDTNTITQRC----------------------GEPNNVGDLEKQPFYRFL 263

Query: 192 HKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVYSLHVTSMVNSVCHIWGNQPWNTGDLS 251
             TY LHPLAL++ LYA+GG PFI+WGM VR V+  H+T +VNS CH+WG Q WNTGDLS
Sbjct: 264 RTTYILHPLALAVALYAMGGFPFIVWGMGVRIVWVYHITWLVNSACHVWGKQAWNTGDLS 323

Query: 252 KNNWMVALLTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYLIRFLQVIGLATNVKLPSEK 311
           KNNW VA L FGEGWHNNHHAFE+SARHG EWWQ+D  WY+++FLQ IGLAT+VKLPSE 
Sbjct: 324 KNNWWVAALAFGEGWHNNHHAFEFSARHGLEWWQLDMTWYVVKFLQAIGLATDVKLPSEA 363

Query: 312 QK 313
           QK
Sbjct: 384 QK 363

BLAST of CSPI07G15670 vs. TAIR 10
Match: AT3G15870.1 (Fatty acid desaturase family protein )

HSP 1 Score: 333.6 bits (854), Expect = 1.8e-91
Identity = 157/301 (52.16%), Postives = 195/301 (64.78%), Query Frame = 0

Query: 11  EMVMKSPPRLFRRKKWTKFDRNVAFYFLSLHLLCILAPFYFSWTCLLLALGLSYLTGMGI 70
           E+V+    R F  + WT +D +    F+  HLL +LAPFYFSW    +   L ++ G+ I
Sbjct: 76  EVVVVRTKRSFWERSWTSWDVSKLVIFVGTHLLSLLAPFYFSWEAFWVFPWLVFINGICI 135

Query: 71  SVSYHRNLAHRSFKLPKWLEYSLAYIATHAIQGDPIDWVSTHRCHHRFVDSARDPHSPIY 130
           ++SYHRNL+HRSF LPKWLEY  AY    A QGDPI+WVS HR HH+  ++ RDPHSP  
Sbjct: 136 TLSYHRNLSHRSFDLPKWLEYLFAYGGVLAFQGDPIEWVSNHRYHHKHCETQRDPHSPTQ 195

Query: 131 GFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIAMFYMKHGYPDNVTDLEKQRFYRFL 190
           GFWFS M W FD+ ++                          G  +NV DL +Q FYRFL
Sbjct: 196 GFWFSHMAWIFDTSSILENC----------------------GGEENVDDLVRQPFYRFL 255

Query: 191 HKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVYSLHVTSMVNSVCHIWGNQPWNTGDLS 250
            +T  LH +A S L Y  GG+P ++WG+ +     LH+T +VNSVCHIWG + WNT D S
Sbjct: 256 QRTVLLHMMAYSFLFYFCGGMPLLVWGIGITIAVRLHLTFLVNSVCHIWGTRAWNTSDFS 315

Query: 251 KNNWMVALLTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYLIRFLQVIGLATNVKLPSEK 310
           KNNW VA+LT GEGWHNNHHAFE+SARHG EWWQ+D  W LIRFL+ IGLATNVKLP+E 
Sbjct: 316 KNNWWVAILTLGEGWHNNHHAFEFSARHGLEWWQLDITWCLIRFLEAIGLATNVKLPTET 354

Query: 311 Q 312
           Q
Sbjct: 376 Q 354

BLAST of CSPI07G15670 vs. TAIR 10
Match: AT1G06080.1 (delta 9 desaturase 1 )

HSP 1 Score: 325.5 bits (833), Expect = 5.0e-89
Identity = 154/294 (52.38%), Postives = 192/294 (65.31%), Query Frame = 0

Query: 19  RLFRRKKWTKFDRNVAFYFLSLHLLCILAPFYFSWTCLLLALGLSYLTGMGISVSYHRNL 78
           R    +KW + D   AF  L +H LC+LAPF F+W  L +AL +  + G+GI+VSYHRNL
Sbjct: 27  RAMWERKWKRLDIVKAFASLFVHFLCLLAPFNFTWPALRVALIVYTVGGLGITVSYHRNL 86

Query: 79  AHRSFKLPKWLEYSLAYIATHAIQGDPIDWVSTHRCHHRFVDSARDPHSPIYGFWFSQMT 138
           AHRSFK+PKWLEY  AY    AIQGDPIDWVSTHR HH+F DS RDPHSP  GFWFS + 
Sbjct: 87  AHRSFKVPKWLEYFFAYCGLLAIQGDPIDWVSTHRYHHQFTDSDRDPHSPNEGFWFSHLL 146

Query: 139 WFFDSYNLTRKVCPNYFNNYQKVERNIAMFYMKHGYPDNVTDLEKQRFYRFLHKTYWLHP 198
           W FD+  L  K                       G   NV DL++Q +Y+FL +T   H 
Sbjct: 147 WLFDTGYLVEKC----------------------GRRTNVEDLKRQWYYKFLQRTVLYHI 206

Query: 199 LALSLLLYAIGGLPFIIWGMCVRFVYSLHVTSMVNSVCHIWGNQPWNTGDLSKNNWMVAL 258
           L    LLY  GGL F+ WGM +      HVT ++NS+CH+WG++ W T D S+N W +++
Sbjct: 207 LTFGFLLYYFGGLSFLTWGMGIGVAMEHHVTCLINSLCHVWGSRTWKTNDTSRNVWWLSV 266

Query: 259 LTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYLIRFLQVIGLATNVKLPSEKQK 313
            +FGE WHNNHHAFE SAR G EWWQID  WY++RFL++IGLAT+VKLPSE Q+
Sbjct: 267 FSFGESWHNNHHAFESSARQGLEWWQIDISWYIVRFLEIIGLATDVKLPSESQR 298

BLAST of CSPI07G15670 vs. TAIR 10
Match: AT2G31360.1 (16:0delta9 desaturase 2 )

HSP 1 Score: 318.9 bits (816), Expect = 4.7e-87
Identity = 150/293 (51.19%), Postives = 191/293 (65.19%), Query Frame = 0

Query: 20  LFRRKKWTKFDRNVAFYFLSLHLLCILAPFYFSWTCLLLALGLSYLTGMGISVSYHRNLA 79
           +F  ++W + D        ++H L +LAPFYF+W+ L +      + G+GI+VSYHRNLA
Sbjct: 30  VFWDRRWRRLDYVKFSASFTVHSLALLAPFYFTWSALWVTFLFYTIGGLGITVSYHRNLA 89

Query: 80  HRSFKLPKWLEYSLAYIATHAIQGDPIDWVSTHRCHHRFVDSARDPHSPIYGFWFSQMTW 139
           HRSFK+PKWLEY LAY A  AIQGDPIDWVSTHR HH+F DS RDPHSP  GFWFS + W
Sbjct: 90  HRSFKVPKWLEYLLAYCALLAIQGDPIDWVSTHRYHHQFTDSERDPHSPKEGFWFSHLLW 149

Query: 140 FFDSYNLTRKVCPNYFNNYQKVERNIAMFYMKHGYPDNVTDLEKQRFYRFLHKTYWLHPL 199
            +DS                      A    K G   NV DL++Q FYRFL KT   H L
Sbjct: 150 IYDS----------------------AYLVSKCGRRANVEDLKRQWFYRFLQKTVLFHIL 209

Query: 200 ALSLLLYAIGGLPFIIWGMCVRFVYSLHVTSMVNSVCHIWGNQPWNTGDLSKNNWMVALL 259
            L   L+ +GG+ F+ WGM V     +HVT ++NS+CHIWG + W T D S+N W +++ 
Sbjct: 210 GLGFFLFYLGGMSFVTWGMGVGAALEVHVTCLINSLCHIWGTRTWKTNDTSRNVWWLSVF 269

Query: 260 TFGEGWHNNHHAFEYSARHGHEWWQIDYGWYLIRFLQVIGLATNVKLPSEKQK 313
           +FGE WHNNHHAFE SAR G EWWQID  WY++RF ++IGLAT+VK+P+E Q+
Sbjct: 270 SFGESWHNNHHAFESSARQGLEWWQIDISWYIVRFFEIIGLATDVKVPTEAQR 300

BLAST of CSPI07G15670 vs. TAIR 10
Match: AT1G06100.1 (Fatty acid desaturase family protein )

HSP 1 Score: 311.2 bits (796), Expect = 9.8e-85
Identity = 159/314 (50.64%), Postives = 199/314 (63.38%), Query Frame = 0

Query: 1   MDEVSKE--KPKEMVMKSPPRLFRRKKWTKFDRNVAFYFLSLHLLCILAPFYFSWTCLLL 60
           M E +K+    ++  ++   R +  +KWT+FD   A    ++HLLC+LAPF + W     
Sbjct: 1   MSETTKDDGSSQKKSVRKEKRAYVLRKWTQFDVGRASTVGTVHLLCLLAPFNYKWEAFRF 60

Query: 61  ALGLSYLTGMGISVSYHRNLAHRSFKLPKWLEYSLAYIATHAIQGDPIDWVSTHRCHHRF 120
            + L+ LT + I+ SYHRNL HRSFKLPKWLEY  AY A  A+QGDP+DWVS HR HH+F
Sbjct: 61  GIILAILTNLCITFSYHRNLTHRSFKLPKWLEYPFAYSALLALQGDPLDWVSIHRFHHQF 120

Query: 121 VDSARDPHSPIYGFWFSQMTWFFDSYNLTRKVCPNYFNNYQKVERNIAMFYMKHGYPDNV 180
            DS RDPHSPI GFWFS + W FD+ +  R+ C                     G  +NV
Sbjct: 121 TDSDRDPHSPIEGFWFSHVLWIFDT-DYIREKC---------------------GRRNNV 180

Query: 181 TDLEKQRFYRFLHKTYWLHPLALSLLLYAIGGLPFIIWGMCVRFVYSLHVTSMVNSVCHI 240
            DL++Q FYRFL KT  LH LA   L+Y  GGLP++ W +    V   H T +VNS CHI
Sbjct: 181 MDLKQQWFYRFLKKTLVLHILAFWTLIYLWGGLPYLTWTVGFGGVIGYHGTWLVNSACHI 240

Query: 241 WGNQPWNTGDLSKNNWMVALLTFGEGWHNNHHAFEYSARHGHEWWQIDYGWYLIRFLQVI 300
            G+Q W T D S+N W +ALLT GE WHNNHHAFE SARHG EW+Q+D  WYLI F Q +
Sbjct: 241 CGSQAWQTNDTSRNVWWLALLTMGESWHNNHHAFETSARHGLEWYQLDITWYLIWFFQAL 292

Query: 301 GLATNVKLPSEKQK 313
           GLATNVKLP++ QK
Sbjct: 301 GLATNVKLPTDAQK 292

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q949X08.6e-10257.28Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic OS=Arab... [more]
Q9LVZ32.6e-9052.16Probable lipid desaturase ADS3.2, chloroplastic OS=Arabidopsis thaliana OX=3702 ... [more]
O657977.1e-8852.38Delta-9 acyl-lipid desaturase 1 OS=Arabidopsis thaliana OX=3702 GN=ADS1 PE=2 SV=... [more]
P0DOW27.8e-8751.18Acyl-CoA C20 Delta5-desaturase OS=Anemone leveillei OX=212809 GN=AL10 PE=1 SV=1[more]
P0DOW35.1e-8655.11Acyl-CoA 5-desaturase AL21 OS=Anemone leveillei OX=212809 GN=AL21 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KAB11.1e-19799.09FA_desaturase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G3763... [more]
A0A1S3BS491.5e-18190.27palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS... [more]
A0A1S3BQG53.4e-17890.27palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS... [more]
A0A6J1JUL61.2e-16283.07palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS... [more]
A0A6J1GPG51.0e-16182.45palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like OS... [more]
Match NameE-valueIdentityDescription
XP_011659261.21.2e-198100.00palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic [Cucumi... [more]
XP_038899717.19.9e-18893.31palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like [B... [more]
XP_008451258.13.1e-18190.27PREDICTED: palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplas... [more]
XP_011659770.15.4e-17889.09palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic [Cucumi... [more]
XP_008451259.17.1e-17890.27PREDICTED: palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplas... [more]
Match NameE-valueIdentityDescription
AT3G15850.16.1e-10357.28fatty acid desaturase 5 [more]
AT3G15870.11.8e-9152.16Fatty acid desaturase family protein [more]
AT1G06080.15.0e-8952.38delta 9 desaturase 1 [more]
AT2G31360.14.7e-8751.1916:0delta9 desaturase 2 [more]
AT1G06100.19.8e-8550.64Fatty acid desaturase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015876Acyl-CoA desaturasePRINTSPR00075FACDDSATRASEcoord: 112..141
score: 44.04
coord: 75..95
score: 37.09
coord: 52..74
score: 20.37
IPR015876Acyl-CoA desaturasePANTHERPTHR11351ACYL-COA DESATURASEcoord: 21..171
coord: 171..313
IPR015876Acyl-CoA desaturaseCDDcd03505Delta9-FADS-likecoord: 66..305
e-value: 6.30303E-68
score: 208.564
IPR005804Fatty acid desaturase domainPFAMPF00487FA_desaturasecoord: 52..290
e-value: 9.9E-14
score: 51.8
NoneNo IPR availablePANTHERPTHR11351:SF94BNAC05G37460D PROTEINcoord: 21..171
coord: 171..313

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI07G15670.1CSPI07G15670.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006633 fatty acid biosynthetic process
biological_process GO:0006629 lipid metabolic process
cellular_component GO:0005789 endoplasmic reticulum membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0009979 16:0 monogalactosyldiacylglycerol desaturase activity
molecular_function GO:0016717 oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water