CSPI07G15650 (gene) Cucumber (PI 183967) v1

BLAST of CSPI07G15650 vs. ExPASy Swiss-Prot
Match: Q9FLV0 (Protein DOWNY MILDEW RESISTANCE 6 OS=Arabidopsis thaliana OX=3702 GN=DMR6 PE=1 SV=1)

HSP 1 Score: 239.6 bits (610), Expect = 5.2e-62
Identity = 131/325 (40.31%), Postives = 197/325 (60.62%), Query Frame = 0

Query: 16  SVPETYVCPPEKR---VGVSVKKKIPVIDLATEDRDLLSKKILDVSQEFGFFQVINHGVS 75
           ++PE YV P   R     VS  +  P+IDL++ DR  L ++I      FGFFQVINHGV+
Sbjct: 14  TLPENYVRPISDRPRLSEVSQLEDFPLIDLSSTDRSFLIQQIHQACARFGFFQVINHGVN 73

Query: 76  KALVEETMRVSREFHAMSSEDKEMECSKDPNKSCLIYSSTPNYEKEELHLWKDSLRLICY 135
           K +++E + V+REF +MS E+K    S DP K+  + S++ N +KEE++ W+D LRL CY
Sbjct: 74  KQIIDEMVSVAREFFSMSMEEKMKLYSDDPTKTTRL-STSFNVKKEEVNNWRDYLRLHCY 133

Query: 136 PNLNKNVQFWPQNPPKFREVMEAYLVVMKKFSLEILEVLSEGLGLEKEYFSGGMSNNPEF 195
           P ++K V  WP NPP F+E++  Y   +++   +I E++SE LGLEK+Y    +    + 
Sbjct: 134 P-IHKYVNEWPSNPPSFKEIVSKYSREVREVGFKIEELISESLGLEKDYMKKVLGEQGQH 193

Query: 196 L-VHHYPPCPNPNLTLGLNKHADPTVITILFQD--VNGLQFLKDGEWIDVDPLPDAFLVN 255
           + V++YPPCP P LT GL  H DP  +TIL QD  V GLQ L DG+W  V+P PDAF++N
Sbjct: 194 MAVNYYPPCPEPELTYGLPAHTDPNALTILLQDTTVCGLQILIDGQWFAVNPHPDAFVIN 253

Query: 256 LGYVFEVISNGKMKAAEHRVVTNTTTSRQSLAYGVYPENNMTIEPSKCF---INEANPPH 315
           +G   + +SNG  K+  HR VTNT   R S+A  + P +   + P+K      ++   P 
Sbjct: 254 IGDQLQALSNGVYKSVWHRAVTNTENPRLSVASFLCPADCAVMSPAKPLWEAEDDETKPV 313

Query: 316 YRTLEFKDFVNKLRSKHPDREMAMK 332
           Y+   + ++  K  S++ D+E  ++
Sbjct: 314 YKDFTYAEYYKKFWSRNLDQEHCLE 336

BLAST of CSPI07G15650 vs. ExPASy Swiss-Prot
Match: P24397 (Hyoscyamine 6-dioxygenase OS=Hyoscyamus niger OX=4079 GN=H6H PE=1 SV=1)

HSP 1 Score: 235.3 bits (599), Expect = 9.9e-61
Identity = 134/342 (39.18%), Postives = 195/342 (57.02%), Query Frame = 0

Query: 7   FVSNWKGIGSVPETYVCPPEKRV--GVSVKKKIPVIDLATEDRDLLSKKILDVSQEFGFF 66
           FVSNW    SV E+++ P +KR    V V   +P+IDL  +   LL ++I    Q+FG F
Sbjct: 4   FVSNW-STKSVSESFIAPLQKRAEKDVPVGNDVPIIDL-QQHHHLLVQQITKACQDFGLF 63

Query: 67  QVINHGVSKALVEETMRVSREFHAMSSEDKEM-----ECSK---DPNKSCLIYSSTPNYE 126
           QVINHG  + L+ ETM V +EF A+ +E+KE      E +K      +   +Y       
Sbjct: 64  QVINHGFPEELMLETMEVCKEFFALPAEEKEKFKPKGEAAKFELPLEQKAKLYVEGEQLS 123

Query: 127 KEELHLWKDSLRLICYPNLNKNVQFWPQNPPKFREVMEAYLVVMKKFSLEILEVLSEGLG 186
            EE   WKD+L   C+P     V  WP+ P K+REV+  Y V ++K ++ +L+ + EGLG
Sbjct: 124 NEEFLYWKDTLAHGCHPLDQDLVNSWPEKPAKYREVVAKYSVEVRKLTMRMLDYICEGLG 183

Query: 187 LEKEYFSGGMSNNPEFLVHHYPPCPNPNLTLGLNKHADPTVITILFQDVNGLQ--FLKDG 246
           L+  YF   +S     L ++YPPCP+P+ TLG   H D  +IT+L QD+ GLQ   +KD 
Sbjct: 184 LKLGYFDNELSQIQMMLTNYYPPCPDPSSTLGSGGHYDGNLITLLQQDLPGLQQLIVKDA 243

Query: 247 EWIDVDPLPDAFLVNLGYVFEVISNGKMKAAEHRVVTNTTTSRQSLAYGVYPENNMTIEP 306
            WI V P+P AF+VNLG   +VI+N K + + HRVVT+ T  R S+A  + P+ + TIEP
Sbjct: 244 TWIAVQPIPTAFVVNLGLTLKVITNEKFEGSIHRVVTDPTRDRVSIATLIGPDYSCTIEP 303

Query: 307 SKCFINEANPPHYRTLEFKDFVNKLRSKHPDREMAMKFISFN 337
           +K  +N+ NPP Y+   + +F +   S   D +  +K    N
Sbjct: 304 AKELLNQDNPPLYKPYSYSEFADIYLSDKSDYDSGVKPYKIN 343

BLAST of CSPI07G15650 vs. ExPASy Swiss-Prot
Match: Q8W2X5 (Flavanone 3-dioxygenase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=F3H-2 PE=1 SV=1)

HSP 1 Score: 228.8 bits (582), Expect = 9.3e-59
Identity = 121/322 (37.58%), Postives = 185/322 (57.45%), Query Frame = 0

Query: 16  SVPETYVCPPEKRVGVSV---KKKIPVIDLATEDRDLLSKKILDVSQEFGFFQVINHGVS 75
           ++P  YV P  +R  + +     +IPV+DLA+ DR  +   + D  +  GFFQV+NHG+ 
Sbjct: 19  TMPGKYVRPESQRPRLDLVVSDARIPVVDLASPDRAAVVSAVGDACRTHGFFQVVNHGID 78

Query: 76  KALVEETMRVSREFHAMSSEDKEMECSKDPNKSCLIYSSTPNYEKEELHLWKDSLRLICY 135
            AL+   M V REF  + +E+K    S DP K   + S++ N  KE +H W+D LRL CY
Sbjct: 79  AALIASVMEVGREFFRLPAEEKAKLYSDDPAKKIRL-STSFNVRKETVHNWRDYLRLHCY 138

Query: 136 PNLNKNVQFWPQNPPKFREVMEAYLVVMKKFSLEILEVLSEGLGLEKEYFSGGMSNNPEF 195
           P L++ V  WP NPP F+E++  Y   +++    + E +SE LGLE  Y    +    + 
Sbjct: 139 P-LHQFVPDWPSNPPSFKEIIGTYCTEVRELGFRLYEAISESLGLEGGYMRETLGEQEQH 198

Query: 196 L-VHHYPPCPNPNLTLGLNKHADPTVITILFQD--VNGLQFLKDGEWIDVDPLPDAFLVN 255
           + V++YP CP P LT GL  H DP  +TIL  D  V GLQ L DG+WI V+P P A ++N
Sbjct: 199 MAVNYYPQCPEPELTYGLPAHTDPNALTILLMDDQVAGLQVLNDGKWIAVNPQPGALVIN 258

Query: 256 LGYVFEVISNGKMKAAEHRVVTNTTTSRQSLAYGVYPENNMTIEPSKCFINEANPPHYRT 315
           +G   + +SNGK ++  HR V N+   R S+A  + P N++ + P+K  I + +P  YR 
Sbjct: 259 IGDQLQALSNGKYRSVWHRAVVNSDRERMSVASFLCPCNSVELGPAKKLITDDSPAVYRN 318

Query: 316 LEFKDFVNKLRSKHPDREMAMK 332
             + ++  K  S++ D+E  ++
Sbjct: 319 YTYDEYYKKFWSRNLDQEHCLE 338

BLAST of CSPI07G15650 vs. ExPASy Swiss-Prot
Match: Q40062 (2'-deoxymugineic-acid 2'-dioxygenase OS=Hordeum vulgare OX=4513 GN=IDS3 PE=1 SV=3)

HSP 1 Score: 214.5 bits (545), Expect = 1.8e-54
Identity = 115/308 (37.34%), Postives = 180/308 (58.44%), Query Frame = 0

Query: 16  SVPETYV-----CPPEKRVGVSVKKKIPVIDLATEDRDLLSKKILDVSQEFGFFQVINHG 75
           S+PE++V      PP  +  VS    +P+IDL+   RD + + IL+  +E GFFQV+NHG
Sbjct: 13  SLPESFVFASDKVPPATKAVVS----LPIIDLSC-GRDEVRRSILEAGKELGFFQVVNHG 72

Query: 76  VSKALVEETMRVSREFHAMSSEDKEMECSKDPNKSCLIYSSTPNYEKEELHLWKDSLRLI 135
           VSK ++ +   +  +F  + + DK    S++ +K   ++S    Y+      W+D LRL 
Sbjct: 73  VSKQVMRDMEGMCEQFFHLPAADKASLYSEERHKPNRLFSGA-TYDTGGEKYWRDCLRLA 132

Query: 136 CYPNLNKNVQFWPQNPPKFREVMEAYLVVMKKFSLEILEVLSEGLGLEKEYFSGGMSNNP 195
           C   ++ ++  WP  P   R+V+E +    +    E+L +L EG+G++ +YF G +S   
Sbjct: 133 CPFPVDDSINEWPDTPKGLRDVIEKFTSQTRDVGKELLRLLCEGMGIQADYFEGDLSGGN 192

Query: 196 EFL-VHHYPPCPNPNLTLGLNKHADPTVITILFQD-VNGLQFLKDGEWIDVDPLPDAFLV 255
             L ++HYP CPNP+  LG   H D  +IT+L    VNGL+    G+WI VDP P+AF+V
Sbjct: 193 VILNINHYPSCPNPDKALGQPPHCDRNLITLLLPGAVNGLEVSYKGDWIKVDPAPNAFVV 252

Query: 256 NLGYVFEVISNGKMKAAEHRVVTNTTTSRQSLAYGVYPENNMTIEPSKCFINEANPPHYR 315
           N G   EV++NG +K+ EHR +TN+  +R S+A  + P     I P+K F+++ NPP YR
Sbjct: 253 NFGQQLEVVTNGLLKSIEHRAMTNSALARTSVATFIMPTQECLIGPAKEFLSKENPPCYR 312

Query: 316 TLEFKDFV 317
           T  F+DF+
Sbjct: 313 TTMFRDFM 314

BLAST of CSPI07G15650 vs. ExPASy Swiss-Prot
Match: Q40061 (Mugineic-acid 3-dioxygenase OS=Hordeum vulgare OX=4513 GN=IDS2 PE=2 SV=1)

HSP 1 Score: 201.4 bits (511), Expect = 1.6e-50
Identity = 108/305 (35.41%), Postives = 177/305 (58.03%), Query Frame = 0

Query: 16  SVPETYVCPPEKRVGVSVKKKIPVIDLATEDRDLLSKKILDVSQEFGFFQVINHGVSKAL 75
           S+P++++ P ++    +    +P+ID+ +  RD + + ILD  +E+GF QV+NHG+S+ +
Sbjct: 14  SIPDSFLLPADRLHPATTDVSLPIIDM-SRGRDEVRQAILDSGKEYGFIQVVNHGISEPM 73

Query: 76  VEETMRVSREFHAMSSEDKEMECSKDPNKSCLIYSSTPNYEKEELHLWKDSLRLICYPNL 135
           + E   V  EF  M +EDK    S+D ++   ++  +  +E      W D L L+ YP  
Sbjct: 74  LHEMYAVCHEFFDMPAEDKAEFFSEDRSERNKLFCGSA-FETLGEKYWIDVLELL-YPLP 133

Query: 136 NKNVQFWPQNPPKFREVMEAYLVVMKKFSLEILEVLSEGLGLEKEYFSGGMSNNPEFL-V 195
           + + + WP  P   REV+  Y  + +  ++EIL +L EGLGL  ++F G +S     + +
Sbjct: 134 SGDTKDWPHKPQMLREVVGNYTSLARGVAMEILRLLCEGLGLRPDFFVGDISGGRVVVDI 193

Query: 196 HHYPPCPNPNLTLGLNKHADPTVITILFQD-VNGLQFLKDGEWIDVDPLPDAFLVNLGYV 255
           ++YPP PNP+ TLGL  H D  ++T+L    V GL+    G WI V P+P++ ++N G  
Sbjct: 194 NYYPPSPNPSRTLGLPPHCDRDLMTVLLPGAVPGLEIAYKGGWIKVQPVPNSLVINFGLQ 253

Query: 256 FEVISNGKMKAAEHRVVTNTTTSRQSLAYGVYPENNMTIEPSKCFINEANPPHYRTLEFK 315
            EV++NG +KA EHR  TN    R S+A  + P ++  + P++ F++E NPP YRTL   
Sbjct: 254 LEVVTNGYLKAVEHRAATNFAEPRLSVASFIVPADDCVVGPAEEFVSEDNPPRYRTLTVG 313

Query: 316 DFVNK 319
           +F  K
Sbjct: 314 EFKRK 315

BLAST of CSPI07G15650 vs. ExPASy TrEMBL
Match: A0A0A0K745 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G375860 PE=3 SV=1)

HSP 1 Score: 696.8 bits (1797), Expect = 4.4e-197
Identity = 336/338 (99.41%), Postives = 338/338 (100.00%), Query Frame = 0

Query: 2   MSQTKFVSNWKGIGSVPETYVCPPEKRVGVSVKKKIPVIDLATEDRDLLSKKILDVSQEF 61
           MSQTKFVSNWKGIGSVPETYVCPPEKRVGVSVKKKIPVIDLATEDRDLLSKK+LDVSQEF
Sbjct: 1   MSQTKFVSNWKGIGSVPETYVCPPEKRVGVSVKKKIPVIDLATEDRDLLSKKVLDVSQEF 60

Query: 62  GFFQVINHGVSKALVEETMRVSREFHAMSSEDKEMECSKDPNKSCLIYSSTPNYEKEELH 121
           GFFQVINHGVSKALVEETMRVSREFHAMSSEDKEMECSKDPNKSCLIYSSTPNYEKEELH
Sbjct: 61  GFFQVINHGVSKALVEETMRVSREFHAMSSEDKEMECSKDPNKSCLIYSSTPNYEKEELH 120

Query: 122 LWKDSLRLICYPNLNKNVQFWPQNPPKFREVMEAYLVVMKKFSLEILEVLSEGLGLEKEY 181
           LWKDSLRLICYPNLNKNVQFWPQNPPKFREVMEAYLVVMKKFSLEILEVLSEGLGLEKEY
Sbjct: 121 LWKDSLRLICYPNLNKNVQFWPQNPPKFREVMEAYLVVMKKFSLEILEVLSEGLGLEKEY 180

Query: 182 FSGGMSNNPEFLVHHYPPCPNPNLTLGLNKHADPTVITILFQDVNGLQFLKDGEWIDVDP 241
           FSGGMSNNPEFLVHHYPPCPNP+LTLGLNKHADPTVITILFQDVNGLQFLKDGEWIDVDP
Sbjct: 181 FSGGMSNNPEFLVHHYPPCPNPSLTLGLNKHADPTVITILFQDVNGLQFLKDGEWIDVDP 240

Query: 242 LPDAFLVNLGYVFEVISNGKMKAAEHRVVTNTTTSRQSLAYGVYPENNMTIEPSKCFINE 301
           LPDAFLVNLGYVFEVISNGKMKAAEHRVVTNTTTSRQSLAYGVYPENNMTIEPSKCFINE
Sbjct: 241 LPDAFLVNLGYVFEVISNGKMKAAEHRVVTNTTTSRQSLAYGVYPENNMTIEPSKCFINE 300

Query: 302 ANPPHYRTLEFKDFVNKLRSKHPDREMAMKFISFNQPY 340
           ANPPHYRTLEFKDFVNKLRSKHPDREMAMKFISFNQPY
Sbjct: 301 ANPPHYRTLEFKDFVNKLRSKHPDREMAMKFISFNQPY 338

BLAST of CSPI07G15650 vs. ExPASy TrEMBL
Match: A0A1S3BRU2 (hyoscyamine 6-dioxygenase-like OS=Cucumis melo OX=3656 GN=LOC103492607 PE=3 SV=1)

HSP 1 Score: 560.5 bits (1443), Expect = 5.0e-156
Identity = 267/339 (78.76%), Postives = 301/339 (88.79%), Query Frame = 0

Query: 1   MMSQTKFVSNWKGIGSVPETYVCPPEKRVGVSVKKKIPVIDLATEDRDLLSKKILDVSQE 60
           MMSQTKFVSNWKG+ SVPETYV PPEKR GVSVKK IPVID A +DR LL KK+ DV+QE
Sbjct: 1   MMSQTKFVSNWKGVESVPETYVYPPEKRFGVSVKKTIPVIDWAIDDRTLLLKKVHDVTQE 60

Query: 61  FGFFQVINHGVSKALVEETMRVSREFHAMSSEDKEMECSKDP-NKSCLIYSSTPNYEKEE 120
           FGF QVINHGV K LVEETM++ +EFHAMS EDKE ECSKDP +KSC +YSS+ NYE E+
Sbjct: 61  FGFVQVINHGVPKTLVEETMKILKEFHAMSPEDKERECSKDPDHKSCRVYSSSQNYETEQ 120

Query: 121 LHLWKDSLRLICYPNLNKNVQFWPQNPPKFREVMEAYLVVMKKFSLEILEVLSEGLGLEK 180
           +HLWKD+L+L C+PNL++NVQFWPQNPPKFREV++AYL+ ++KF+LEILEVLSEGLGLEK
Sbjct: 121 IHLWKDTLKLTCHPNLDENVQFWPQNPPKFREVVKAYLLKIEKFALEILEVLSEGLGLEK 180

Query: 181 EYFSGGMSNNPEFLVHHYPPCPNPNLTLGLNKHADPTVITILFQDVNGLQFLKDGEWIDV 240
            Y  G MS NPE  VHHYPPCPNP+LT+GLN+HADPT+ITILFQDVNGLQFLKDG+WIDV
Sbjct: 181 GYLRGEMSKNPELFVHHYPPCPNPSLTIGLNQHADPTLITILFQDVNGLQFLKDGQWIDV 240

Query: 241 DPLPDAFLVNLGYVFEVISNGKMKAAEHRVVTNTTTSRQSLAYGVYPENNMTIEPSKCFI 300
           DPLP+AFLVNLGYV EVISNGKMKA EHRVVTNTTTSRQSLAY VYP N++ IEPSKCFI
Sbjct: 241 DPLPNAFLVNLGYVLEVISNGKMKAVEHRVVTNTTTSRQSLAYVVYPGNDVNIEPSKCFI 300

Query: 301 NEANPPHYRTLEFKDFVNKLRSKHPDREMAMKFISFNQP 339
           NEANPPHY +LEFKDF+ KL SK PDRE A+KFISFNQP
Sbjct: 301 NEANPPHYLSLEFKDFIKKLMSKPPDRETALKFISFNQP 339

BLAST of CSPI07G15650 vs. ExPASy TrEMBL
Match: A0A5A7V1J0 (Hyoscyamine 6-dioxygenase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold886G00760 PE=3 SV=1)

HSP 1 Score: 558.5 bits (1438), Expect = 1.9e-155
Identity = 266/339 (78.47%), Postives = 301/339 (88.79%), Query Frame = 0

Query: 1   MMSQTKFVSNWKGIGSVPETYVCPPEKRVGVSVKKKIPVIDLATEDRDLLSKKILDVSQE 60
           MMSQTKFVSNWKG+ SVPETYV PPEKR G+SVKK IPVID A +DR LL KK+ DV++E
Sbjct: 1   MMSQTKFVSNWKGVESVPETYVYPPEKRFGISVKKTIPVIDWAIDDRALLLKKVHDVTRE 60

Query: 61  FGFFQVINHGVSKALVEETMRVSREFHAMSSEDKEMECSKDP-NKSCLIYSSTPNYEKEE 120
           FGF QVINHGV K LVEETM+V +EFHAMS EDKE ECSKDP +KSC +YSS+ NYE E+
Sbjct: 61  FGFVQVINHGVPKTLVEETMKVLKEFHAMSPEDKERECSKDPDHKSCRVYSSSQNYETEQ 120

Query: 121 LHLWKDSLRLICYPNLNKNVQFWPQNPPKFREVMEAYLVVMKKFSLEILEVLSEGLGLEK 180
           +HLWKD+L+L C+PNL++NVQFWPQNPPKFREV++AYL+ ++KF+LEILEVLSEGLGLEK
Sbjct: 121 IHLWKDTLKLTCHPNLDENVQFWPQNPPKFREVVKAYLLKIEKFALEILEVLSEGLGLEK 180

Query: 181 EYFSGGMSNNPEFLVHHYPPCPNPNLTLGLNKHADPTVITILFQDVNGLQFLKDGEWIDV 240
            Y  G MS NPE  VHHYPPCPNP+LT+GLN+HADPT+ITILFQDVNGLQFLKDG+WIDV
Sbjct: 181 GYLRGEMSKNPELFVHHYPPCPNPSLTIGLNQHADPTLITILFQDVNGLQFLKDGQWIDV 240

Query: 241 DPLPDAFLVNLGYVFEVISNGKMKAAEHRVVTNTTTSRQSLAYGVYPENNMTIEPSKCFI 300
           DPLP+AFLVNLGYV EVISNGKMKA EHRVVTNTTTSRQSLAY VYP N++ IEPSKCFI
Sbjct: 241 DPLPNAFLVNLGYVLEVISNGKMKAVEHRVVTNTTTSRQSLAYVVYPGNDVNIEPSKCFI 300

Query: 301 NEANPPHYRTLEFKDFVNKLRSKHPDREMAMKFISFNQP 339
           NEANPPHY +LEFKDF+ KL SK PDRE A+KFISFNQP
Sbjct: 301 NEANPPHYLSLEFKDFIKKLMSKPPDRETALKFISFNQP 339

BLAST of CSPI07G15650 vs. ExPASy TrEMBL
Match: A0A0A0K5H0 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G375850 PE=3 SV=1)

HSP 1 Score: 548.1 bits (1411), Expect = 2.5e-152
Identity = 256/337 (75.96%), Postives = 302/337 (89.61%), Query Frame = 0

Query: 1   MMSQTKFVSNWKGIGSVPETYVCPPEKRVGVSVKKKIPVIDLATEDRDLLSKKILDVSQE 60
           MMS+TKFVSNW G+ SVPETYV PPEKR G+SVKK IPVID  ++DR LL K++ DV+QE
Sbjct: 1   MMSRTKFVSNWGGVVSVPETYVYPPEKRFGISVKKSIPVIDW-SDDRALLLKRVHDVTQE 60

Query: 61  FGFFQVINHGVSKALVEETMRVSREFHAMSSEDKEMECSKDPNKSCLIYSSTPNYEKEEL 120
           FGF QVINHGV K LVEE M++ +EFH MS+EDKE ECSKDP+KSC +Y+S+ NYE E++
Sbjct: 61  FGFVQVINHGVPKTLVEEMMKIMKEFHGMSAEDKERECSKDPDKSCKVYTSSQNYENEQI 120

Query: 121 HLWKDSLRLICYPNLNKNVQFWPQNPPKFREVMEAYLVVMKKFSLEILEVLSEGLGLEKE 180
           HLWKD+ +L C+PNL+KN++FWPQNPPK REV++AY+V+++KF+ EILEVLSEGLGLEK 
Sbjct: 121 HLWKDTFKLRCHPNLDKNLEFWPQNPPKLREVVKAYIVMIEKFASEILEVLSEGLGLEKG 180

Query: 181 YFSGGMSNNPEFLVHHYPPCPNPNLTLGLNKHADPTVITILFQDVNGLQFLKDGEWIDVD 240
           YF G MS NPEFL HHYPPCPNP+LT+GL++HADPT+ITILFQDVNGLQFLKDG+WIDVD
Sbjct: 181 YFRGEMSKNPEFLAHHYPPCPNPSLTVGLSQHADPTLITILFQDVNGLQFLKDGQWIDVD 240

Query: 241 PLPDAFLVNLGYVFEVISNGKMKAAEHRVVTNTTTSRQSLAYGVYPENNMTIEPSKCFIN 300
           PLPDAFLVNLGYVFEVISNGK+KAAEHRVVTN+TTSRQ+L Y VYPEN++TIEPSKCFIN
Sbjct: 241 PLPDAFLVNLGYVFEVISNGKLKAAEHRVVTNSTTSRQTLVYVVYPENDVTIEPSKCFIN 300

Query: 301 EANPPHYRTLEFKDFVNKLRSKHPDREMAMKFISFNQ 338
           EANPPHYR+LEFKDF NKL SK PDR+ AM+FISF+Q
Sbjct: 301 EANPPHYRSLEFKDFYNKLMSKPPDRKTAMEFISFDQ 336

BLAST of CSPI07G15650 vs. ExPASy TrEMBL
Match: A0A0A0K5H3 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G378370 PE=3 SV=1)

HSP 1 Score: 435.6 bits (1119), Expect = 1.8e-118
Identity = 206/335 (61.49%), Postives = 260/335 (77.61%), Query Frame = 0

Query: 6   KFVSNWKGIGSVPETYVCPPEKRVG---VSVKKKIPVIDLATEDRDLLSKKILDVSQEFG 65
           KFVSNW+ + SVPE+YV PPEKR G   V + K IPVIDL+  DR LL +KILD S+EFG
Sbjct: 7   KFVSNWETVESVPESYVYPPEKRPGNIVVPMAKAIPVIDLSIRDRTLLVRKILDASKEFG 66

Query: 66  FFQVINHGVSKALVEETMRVSREFHAMSSEDKEMECSKDPNKSCLIYSSTPNYEKEELHL 125
           FFQ+INHGVSK + EETMR+ +EFHAMS  +K  ECSKDPN+SC +Y+S+ NY KE++H 
Sbjct: 67  FFQIINHGVSKKVSEETMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYTKEQVHC 126

Query: 126 WKDSLRLICYPNLNKNVQFWPQNPPKFREVMEAYLVVMKKFSLEILEVLSEGLGLEKEYF 185
           W+D+L   C+P L K V FWPQ PPK+REV+ AY + M+K  LEILE++SEGLGL K YF
Sbjct: 127 WRDALIFNCHP-LEKYVHFWPQKPPKYREVVGAYCMAMRKLVLEILELMSEGLGLGKGYF 186

Query: 186 SGGMSNNPEFLVHHYPPCPNPNLTLGLNKHADPTVITILFQDVNGLQFLKDGEWIDVDPL 245
            G MS NP  LV+HYPPCPNP+LTLGL++H DP++ITILFQDVNGLQ LKDG+WI V P+
Sbjct: 187 GGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWIGVQPI 246

Query: 246 PDAFLVNLGYVFEVISNGKMKAAEHRVVTNTTTSRQSLAYGVYPENNMTIEPSKCFINEA 305
            +AF+VN+G+V ++I+NGK++AAEHR VTN+ TSRQSL Y VYP++   +EP+KC INEA
Sbjct: 247 DNAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKCMINEA 306

Query: 306 NPPHYRTLEFKDFVNKLRSKHPDREMAMKFISFNQ 338
           NPP YR+L FKDF      +  D +  M++I  NQ
Sbjct: 307 NPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQ 340

BLAST of CSPI07G15650 vs. NCBI nr
Match: XP_011659768.2 (protein DOWNY MILDEW RESISTANCE 6 [Cucumis sativus] >KAE8646291.1 hypothetical protein Csa_015678 [Cucumis sativus])

HSP 1 Score: 698.7 bits (1802), Expect = 2.4e-197
Identity = 337/339 (99.41%), Postives = 339/339 (100.00%), Query Frame = 0

Query: 1   MMSQTKFVSNWKGIGSVPETYVCPPEKRVGVSVKKKIPVIDLATEDRDLLSKKILDVSQE 60
           MMSQTKFVSNWKGIGSVPETYVCPPEKRVGVSVKKKIPVIDLATEDRDLLSKK+LDVSQE
Sbjct: 1   MMSQTKFVSNWKGIGSVPETYVCPPEKRVGVSVKKKIPVIDLATEDRDLLSKKVLDVSQE 60

Query: 61  FGFFQVINHGVSKALVEETMRVSREFHAMSSEDKEMECSKDPNKSCLIYSSTPNYEKEEL 120
           FGFFQVINHGVSKALVEETMRVSREFHAMSSEDKEMECSKDPNKSCLIYSSTPNYEKEEL
Sbjct: 61  FGFFQVINHGVSKALVEETMRVSREFHAMSSEDKEMECSKDPNKSCLIYSSTPNYEKEEL 120

Query: 121 HLWKDSLRLICYPNLNKNVQFWPQNPPKFREVMEAYLVVMKKFSLEILEVLSEGLGLEKE 180
           HLWKDSLRLICYPNLNKNVQFWPQNPPKFREVMEAYLVVMKKFSLEILEVLSEGLGLEKE
Sbjct: 121 HLWKDSLRLICYPNLNKNVQFWPQNPPKFREVMEAYLVVMKKFSLEILEVLSEGLGLEKE 180

Query: 181 YFSGGMSNNPEFLVHHYPPCPNPNLTLGLNKHADPTVITILFQDVNGLQFLKDGEWIDVD 240
           YFSGGMSNNPEFLVHHYPPCPNP+LTLGLNKHADPTVITILFQDVNGLQFLKDGEWIDVD
Sbjct: 181 YFSGGMSNNPEFLVHHYPPCPNPSLTLGLNKHADPTVITILFQDVNGLQFLKDGEWIDVD 240

Query: 241 PLPDAFLVNLGYVFEVISNGKMKAAEHRVVTNTTTSRQSLAYGVYPENNMTIEPSKCFIN 300
           PLPDAFLVNLGYVFEVISNGKMKAAEHRVVTNTTTSRQSLAYGVYPENNMTIEPSKCFIN
Sbjct: 241 PLPDAFLVNLGYVFEVISNGKMKAAEHRVVTNTTTSRQSLAYGVYPENNMTIEPSKCFIN 300

Query: 301 EANPPHYRTLEFKDFVNKLRSKHPDREMAMKFISFNQPY 340
           EANPPHYRTLEFKDFVNKLRSKHPDREMAMKFISFNQPY
Sbjct: 301 EANPPHYRTLEFKDFVNKLRSKHPDREMAMKFISFNQPY 339

BLAST of CSPI07G15650 vs. NCBI nr
Match: XP_008451257.1 (PREDICTED: hyoscyamine 6-dioxygenase-like [Cucumis melo])

HSP 1 Score: 560.5 bits (1443), Expect = 1.0e-155
Identity = 267/339 (78.76%), Postives = 301/339 (88.79%), Query Frame = 0

Query: 1   MMSQTKFVSNWKGIGSVPETYVCPPEKRVGVSVKKKIPVIDLATEDRDLLSKKILDVSQE 60
           MMSQTKFVSNWKG+ SVPETYV PPEKR GVSVKK IPVID A +DR LL KK+ DV+QE
Sbjct: 1   MMSQTKFVSNWKGVESVPETYVYPPEKRFGVSVKKTIPVIDWAIDDRTLLLKKVHDVTQE 60

Query: 61  FGFFQVINHGVSKALVEETMRVSREFHAMSSEDKEMECSKDP-NKSCLIYSSTPNYEKEE 120
           FGF QVINHGV K LVEETM++ +EFHAMS EDKE ECSKDP +KSC +YSS+ NYE E+
Sbjct: 61  FGFVQVINHGVPKTLVEETMKILKEFHAMSPEDKERECSKDPDHKSCRVYSSSQNYETEQ 120

Query: 121 LHLWKDSLRLICYPNLNKNVQFWPQNPPKFREVMEAYLVVMKKFSLEILEVLSEGLGLEK 180
           +HLWKD+L+L C+PNL++NVQFWPQNPPKFREV++AYL+ ++KF+LEILEVLSEGLGLEK
Sbjct: 121 IHLWKDTLKLTCHPNLDENVQFWPQNPPKFREVVKAYLLKIEKFALEILEVLSEGLGLEK 180

Query: 181 EYFSGGMSNNPEFLVHHYPPCPNPNLTLGLNKHADPTVITILFQDVNGLQFLKDGEWIDV 240
            Y  G MS NPE  VHHYPPCPNP+LT+GLN+HADPT+ITILFQDVNGLQFLKDG+WIDV
Sbjct: 181 GYLRGEMSKNPELFVHHYPPCPNPSLTIGLNQHADPTLITILFQDVNGLQFLKDGQWIDV 240

Query: 241 DPLPDAFLVNLGYVFEVISNGKMKAAEHRVVTNTTTSRQSLAYGVYPENNMTIEPSKCFI 300
           DPLP+AFLVNLGYV EVISNGKMKA EHRVVTNTTTSRQSLAY VYP N++ IEPSKCFI
Sbjct: 241 DPLPNAFLVNLGYVLEVISNGKMKAVEHRVVTNTTTSRQSLAYVVYPGNDVNIEPSKCFI 300

Query: 301 NEANPPHYRTLEFKDFVNKLRSKHPDREMAMKFISFNQP 339
           NEANPPHY +LEFKDF+ KL SK PDRE A+KFISFNQP
Sbjct: 301 NEANPPHYLSLEFKDFIKKLMSKPPDRETALKFISFNQP 339

BLAST of CSPI07G15650 vs. NCBI nr
Match: KAA0059649.1 (hyoscyamine 6-dioxygenase-like [Cucumis melo var. makuwa] >TYK08181.1 hyoscyamine 6-dioxygenase-like [Cucumis melo var. makuwa])

HSP 1 Score: 558.5 bits (1438), Expect = 3.9e-155
Identity = 266/339 (78.47%), Postives = 301/339 (88.79%), Query Frame = 0

Query: 1   MMSQTKFVSNWKGIGSVPETYVCPPEKRVGVSVKKKIPVIDLATEDRDLLSKKILDVSQE 60
           MMSQTKFVSNWKG+ SVPETYV PPEKR G+SVKK IPVID A +DR LL KK+ DV++E
Sbjct: 1   MMSQTKFVSNWKGVESVPETYVYPPEKRFGISVKKTIPVIDWAIDDRALLLKKVHDVTRE 60

Query: 61  FGFFQVINHGVSKALVEETMRVSREFHAMSSEDKEMECSKDP-NKSCLIYSSTPNYEKEE 120
           FGF QVINHGV K LVEETM+V +EFHAMS EDKE ECSKDP +KSC +YSS+ NYE E+
Sbjct: 61  FGFVQVINHGVPKTLVEETMKVLKEFHAMSPEDKERECSKDPDHKSCRVYSSSQNYETEQ 120

Query: 121 LHLWKDSLRLICYPNLNKNVQFWPQNPPKFREVMEAYLVVMKKFSLEILEVLSEGLGLEK 180
           +HLWKD+L+L C+PNL++NVQFWPQNPPKFREV++AYL+ ++KF+LEILEVLSEGLGLEK
Sbjct: 121 IHLWKDTLKLTCHPNLDENVQFWPQNPPKFREVVKAYLLKIEKFALEILEVLSEGLGLEK 180

Query: 181 EYFSGGMSNNPEFLVHHYPPCPNPNLTLGLNKHADPTVITILFQDVNGLQFLKDGEWIDV 240
            Y  G MS NPE  VHHYPPCPNP+LT+GLN+HADPT+ITILFQDVNGLQFLKDG+WIDV
Sbjct: 181 GYLRGEMSKNPELFVHHYPPCPNPSLTIGLNQHADPTLITILFQDVNGLQFLKDGQWIDV 240

Query: 241 DPLPDAFLVNLGYVFEVISNGKMKAAEHRVVTNTTTSRQSLAYGVYPENNMTIEPSKCFI 300
           DPLP+AFLVNLGYV EVISNGKMKA EHRVVTNTTTSRQSLAY VYP N++ IEPSKCFI
Sbjct: 241 DPLPNAFLVNLGYVLEVISNGKMKAVEHRVVTNTTTSRQSLAYVVYPGNDVNIEPSKCFI 300

Query: 301 NEANPPHYRTLEFKDFVNKLRSKHPDREMAMKFISFNQP 339
           NEANPPHY +LEFKDF+ KL SK PDRE A+KFISFNQP
Sbjct: 301 NEANPPHYLSLEFKDFIKKLMSKPPDRETALKFISFNQP 339

BLAST of CSPI07G15650 vs. NCBI nr
Match: XP_004148957.1 (hyoscyamine 6-dioxygenase [Cucumis sativus] >KGN44743.1 hypothetical protein Csa_016242 [Cucumis sativus])

HSP 1 Score: 548.1 bits (1411), Expect = 5.3e-152
Identity = 256/337 (75.96%), Postives = 302/337 (89.61%), Query Frame = 0

Query: 1   MMSQTKFVSNWKGIGSVPETYVCPPEKRVGVSVKKKIPVIDLATEDRDLLSKKILDVSQE 60
           MMS+TKFVSNW G+ SVPETYV PPEKR G+SVKK IPVID  ++DR LL K++ DV+QE
Sbjct: 1   MMSRTKFVSNWGGVVSVPETYVYPPEKRFGISVKKSIPVIDW-SDDRALLLKRVHDVTQE 60

Query: 61  FGFFQVINHGVSKALVEETMRVSREFHAMSSEDKEMECSKDPNKSCLIYSSTPNYEKEEL 120
           FGF QVINHGV K LVEE M++ +EFH MS+EDKE ECSKDP+KSC +Y+S+ NYE E++
Sbjct: 61  FGFVQVINHGVPKTLVEEMMKIMKEFHGMSAEDKERECSKDPDKSCKVYTSSQNYENEQI 120

Query: 121 HLWKDSLRLICYPNLNKNVQFWPQNPPKFREVMEAYLVVMKKFSLEILEVLSEGLGLEKE 180
           HLWKD+ +L C+PNL+KN++FWPQNPPK REV++AY+V+++KF+ EILEVLSEGLGLEK 
Sbjct: 121 HLWKDTFKLRCHPNLDKNLEFWPQNPPKLREVVKAYIVMIEKFASEILEVLSEGLGLEKG 180

Query: 181 YFSGGMSNNPEFLVHHYPPCPNPNLTLGLNKHADPTVITILFQDVNGLQFLKDGEWIDVD 240
           YF G MS NPEFL HHYPPCPNP+LT+GL++HADPT+ITILFQDVNGLQFLKDG+WIDVD
Sbjct: 181 YFRGEMSKNPEFLAHHYPPCPNPSLTVGLSQHADPTLITILFQDVNGLQFLKDGQWIDVD 240

Query: 241 PLPDAFLVNLGYVFEVISNGKMKAAEHRVVTNTTTSRQSLAYGVYPENNMTIEPSKCFIN 300
           PLPDAFLVNLGYVFEVISNGK+KAAEHRVVTN+TTSRQ+L Y VYPEN++TIEPSKCFIN
Sbjct: 241 PLPDAFLVNLGYVFEVISNGKLKAAEHRVVTNSTTSRQTLVYVVYPENDVTIEPSKCFIN 300

Query: 301 EANPPHYRTLEFKDFVNKLRSKHPDREMAMKFISFNQ 338
           EANPPHYR+LEFKDF NKL SK PDR+ AM+FISF+Q
Sbjct: 301 EANPPHYRSLEFKDFYNKLMSKPPDRKTAMEFISFDQ 336

BLAST of CSPI07G15650 vs. NCBI nr
Match: XP_038897058.1 (hyoscyamine 6-dioxygenase-like [Benincasa hispida])

HSP 1 Score: 436.8 bits (1122), Expect = 1.7e-118
Identity = 209/340 (61.47%), Postives = 261/340 (76.76%), Query Frame = 0

Query: 1   MMSQTKFVSNWKGIGSVPETYVCPPEKRVG---VSVKKKIPVIDLATEDRDLLSKKILDV 60
           M    KFV+NW+G+ SVPE+YV PP KR G   V ++  IPVIDL+ +DR+LL +KILD 
Sbjct: 1   MGDANKFVANWEGVKSVPESYVYPPGKRPGNVVVPMENAIPVIDLSMQDRNLLIRKILDA 60

Query: 61  SQEFGFFQVINHGVSKALVEETMRVSREFHAMSSEDKEMECSKDPNKSCLIYSSTPNYEK 120
           S+EFGFFQVINHGVSK +  ETMR+ +EFHAMS  +K  ECSKDPN+SC +Y+S+ NY K
Sbjct: 61  SKEFGFFQVINHGVSKTVSGETMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYMK 120

Query: 121 EELHLWKDSLRLICYPNLNKNVQFWPQNPPKFREVMEAYLVVMKKFSLEILEVLSEGLGL 180
           E++H W+D+L   C+P L K V FWPQNPPK+REV+ AY V M+K  LEILE++SEGLGL
Sbjct: 121 EQIHCWRDALIFNCHP-LEKYVHFWPQNPPKYREVVGAYCVAMRKLVLEILELMSEGLGL 180

Query: 181 EKEYFSGGMSNNPEFLVHHYPPCPNPNLTLGLNKHADPTVITILFQDVNGLQFLKDGEWI 240
            K YF G MS NP  LV+HYPPCPNP+LTLGL++H DP++ITILFQDVNGLQ LKDG WI
Sbjct: 181 GKGYFGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGRWI 240

Query: 241 DVDPLPDAFLVNLGYVFEVISNGKMKAAEHRVVTNTTTSRQSLAYGVYPENNMTIEPSKC 300
            V P+ +AF+VN+G+V +VI+NGK+KAAEHR VTN  TSRQSL Y VYP++  T+EP+KC
Sbjct: 241 GVQPIDNAFVVNIGFVLQVITNGKLKAAEHRAVTNAKTSRQSLTYLVYPKDEATMEPAKC 300

Query: 301 FINEANPPHYRTLEFKDFVNKLRSKHPDREMAMKFISFNQ 338
            INEANP  YR+L FKDF      +  D +  M++I  NQ
Sbjct: 301 MINEANPRRYRSLNFKDFQRNYLPRAVDTKAVMEYIGSNQ 339

BLAST of CSPI07G15650 vs. TAIR 10
Match: AT5G24530.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 239.6 bits (610), Expect = 3.7e-63
Identity = 131/325 (40.31%), Postives = 197/325 (60.62%), Query Frame = 0

Query: 16  SVPETYVCPPEKR---VGVSVKKKIPVIDLATEDRDLLSKKILDVSQEFGFFQVINHGVS 75
           ++PE YV P   R     VS  +  P+IDL++ DR  L ++I      FGFFQVINHGV+
Sbjct: 14  TLPENYVRPISDRPRLSEVSQLEDFPLIDLSSTDRSFLIQQIHQACARFGFFQVINHGVN 73

Query: 76  KALVEETMRVSREFHAMSSEDKEMECSKDPNKSCLIYSSTPNYEKEELHLWKDSLRLICY 135
           K +++E + V+REF +MS E+K    S DP K+  + S++ N +KEE++ W+D LRL CY
Sbjct: 74  KQIIDEMVSVAREFFSMSMEEKMKLYSDDPTKTTRL-STSFNVKKEEVNNWRDYLRLHCY 133

Query: 136 PNLNKNVQFWPQNPPKFREVMEAYLVVMKKFSLEILEVLSEGLGLEKEYFSGGMSNNPEF 195
           P ++K V  WP NPP F+E++  Y   +++   +I E++SE LGLEK+Y    +    + 
Sbjct: 134 P-IHKYVNEWPSNPPSFKEIVSKYSREVREVGFKIEELISESLGLEKDYMKKVLGEQGQH 193

Query: 196 L-VHHYPPCPNPNLTLGLNKHADPTVITILFQD--VNGLQFLKDGEWIDVDPLPDAFLVN 255
           + V++YPPCP P LT GL  H DP  +TIL QD  V GLQ L DG+W  V+P PDAF++N
Sbjct: 194 MAVNYYPPCPEPELTYGLPAHTDPNALTILLQDTTVCGLQILIDGQWFAVNPHPDAFVIN 253

Query: 256 LGYVFEVISNGKMKAAEHRVVTNTTTSRQSLAYGVYPENNMTIEPSKCF---INEANPPH 315
           +G   + +SNG  K+  HR VTNT   R S+A  + P +   + P+K      ++   P 
Sbjct: 254 IGDQLQALSNGVYKSVWHRAVTNTENPRLSVASFLCPADCAVMSPAKPLWEAEDDETKPV 313

Query: 316 YRTLEFKDFVNKLRSKHPDREMAMK 332
           Y+   + ++  K  S++ D+E  ++
Sbjct: 314 YKDFTYAEYYKKFWSRNLDQEHCLE 336

BLAST of CSPI07G15650 vs. TAIR 10
Match: AT4G10500.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 200.7 bits (509), Expect = 1.9e-51
Identity = 115/321 (35.83%), Postives = 177/321 (55.14%), Query Frame = 0

Query: 8   VSNWKGIGSVPETYVCPPEKRVGVSVKK----KIPVIDLAT---EDRDLLSKKILDVSQE 67
           VS++     +P  YV P   R  +S  +     IP+IDL      +R ++ +++      
Sbjct: 11  VSDFASSVHIPSNYVRPISDRPNLSEVESSGDSIPLIDLRDLHGPNRAVIVQQLASACST 70

Query: 68  FGFFQVINHGVSKALVEETMRVSREFHAMSSEDKEMECSKDPNKSCLIYSSTPNYEKEEL 127
           +GFFQ+ NHGV    V +   V+REF      ++    S DP K+  + S++ N   +++
Sbjct: 71  YGFFQIKNHGVPDTTVNKMQTVAREFFHQPESERVKHYSADPTKTTRL-STSFNVGADKV 130

Query: 128 HLWKDSLRLICYPNLNKNVQFWPQNPPKFREVMEAYLVVMKKFSLEILEVLSEGLGLEKE 187
             W+D LRL C+P +   ++ WP +P  FREV   Y   ++   L +LE +SE LGLE +
Sbjct: 131 LNWRDFLRLHCFP-IEDFIEEWPSSPISFREVTAEYATSVRALVLRLLEAISESLGLESD 190

Query: 188 YFSGGMSNNPEFLV-HHYPPCPNPNLTLGLNKHADPTVITILFQD-VNGLQFLKDGEWID 247
           + S  +  + + +  ++YPPCP P LT GL  H DPTVIT+L QD V+GLQ  KD +W+ 
Sbjct: 191 HISNILGKHAQHMAFNYYPPCPEPELTYGLPGHKDPTVITVLLQDQVSGLQVFKDDKWVA 250

Query: 248 VDPLPDAFLVNLGYVFEVISNGKMKAAEHRVVTNTTTSRQSLAYGVYPENNMTIEPSKCF 307
           V P+P+ F+VN+G   +VISN K K+  HR V NT   R S+    +P  +  I P+   
Sbjct: 251 VSPIPNTFIVNIGDQMQVISNDKYKSVLHRAVVNTENERLSIPTFYFPSTDAVIGPAHEL 310

Query: 308 INEANP-PHYRTLEFKDFVNK 319
           +NE +    YRT  F ++ +K
Sbjct: 311 VNEQDSLAIYRTYPFVEYWDK 329

BLAST of CSPI07G15650 vs. TAIR 10
Match: AT4G10490.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 193.4 bits (490), Expect = 3.1e-49
Identity = 117/320 (36.56%), Postives = 171/320 (53.44%), Query Frame = 0

Query: 14  IGSVPETYVCPPEKRVGVSVKK----KIPVIDLATEDRDLLSKKILDVSQEF-------G 73
           +  VP  YV P   R  +S  +     IP+IDL     DL      D+  +F       G
Sbjct: 15  VDHVPSNYVRPVSDRPKMSEVQTSGDSIPLIDL----HDLHGPNRADIINQFAHACSSCG 74

Query: 74  FFQVINHGVSKALVEETMRVSREFHAMSSEDKEMECSKDPNKSCLIYSSTPNYEKEELHL 133
           FFQ+ NHGV +  +++ M  +REF   S  ++    S D  K+  + S++ N  KE++  
Sbjct: 75  FFQIKNHGVPEETIKKMMNAAREFFRQSESERVKHYSADTKKTTRL-STSFNVSKEKVSN 134

Query: 134 WKDSLRLICYPNLNKNVQFWPQNPPKFREVMEAYLVVMKKFSLEILEVLSEGLGLEKEYF 193
           W+D LRL CYP +   +  WP  P  FREV   Y   ++   L +LE +SE LGL K+  
Sbjct: 135 WRDFLRLHCYP-IEDFINEWPSTPISFREVTAEYATSVRALVLTLLEAISESLGLAKDRV 194

Query: 194 SGGMSNNPEFL-VHHYPPCPNPNLTLGLNKHADPTVITILFQD-VNGLQFLKDGEWIDVD 253
           S  +  + + + +++YP CP P LT GL  H D  +IT+L QD V+GLQ  KDG+WI V+
Sbjct: 195 SNTIGKHGQHMAINYYPRCPQPELTYGLPGHKDANLITVLLQDEVSGLQVFKDGKWIAVN 254

Query: 254 PLPDAFLVNLGYVFEVISNGKMKAAEHRVVTNTTTSRQSLAYGVYPENNMTIEPSKCFIN 313
           P+P+ F+VNLG   +VISN K K+  HR V N+   R S+     P  +  I P++  IN
Sbjct: 255 PVPNTFIVNLGDQMQVISNEKYKSVLHRAVVNSDMERISIPTFYCPSEDAVISPAQELIN 314

Query: 314 EA--NPPHYRTLEFKDFVNK 319
           E   +P  YR   + ++  K
Sbjct: 315 EEEDSPAIYRNFTYAEYFEK 328

BLAST of CSPI07G15650 vs. TAIR 10
Match: AT5G08640.1 (flavonol synthase 1 )

HSP 1 Score: 184.9 bits (468), Expect = 1.1e-46
Identity = 98/292 (33.56%), Postives = 157/292 (53.77%), Query Frame = 0

Query: 37  IPVIDLATEDRDLLSKKILDVSQEFGFFQVINHGVSKALVEETMRVSREFHAMSSEDKEM 96
           IPV+DL+  D + + + ++  S+E+G FQV+NHG+   L+     V R+F  + S +KE 
Sbjct: 43  IPVVDLSDPDEESVRRAVVKASEEWGLFQVVNHGIPTELIRRLQDVGRKFFELPSSEKES 102

Query: 97  ECSKDPNKSCLIYSSTPNYEKEELHLWKDSLRLICYPNLNKNVQFWPQNPPKFREVMEAY 156
               + +K    Y +    + E    W D L    +P    N +FWP+NPP++REV E Y
Sbjct: 103 VAKPEDSKDIEGYGTKLQKDPEGKKAWVDHLFHRIWPPSCVNYRFWPKNPPEYREVNEEY 162

Query: 157 LVVMKKFSLEILEVLSEGLGLEKEYFSGGMSNN-PEFL--VHHYPPCPNPNLTLGLNKHA 216
            V +KK S  +L +LS+GLGL+++    G+     E++  +++YPPCP P+L LG+  H 
Sbjct: 163 AVHVKKLSETLLGILSDGLGLKRDALKEGLGGEMAEYMMKINYYPPCPRPDLALGVPAHT 222

Query: 217 DPTVITILF-QDVNGLQFLKDGEWIDVDPLPDAFLVNLGYVFEVISNGKMKAAEHRVVTN 276
           D + IT+L   +V GLQ  KD  W D + +P A +V++G     +SNG+ K   HR   +
Sbjct: 223 DLSGITLLVPNEVPGLQVFKDDHWFDAEYIPSAVIVHIGDQILRLSNGRYKNVLHRTTVD 282

Query: 277 TTTSRQSLAYGVYPENNMTIEPSKCFINEANPPHYRTLEFKDFVNKLRSKHP 325
              +R S    + P     + P      + NPP ++   FKD+  +  +K P
Sbjct: 283 KEKTRMSWPVFLEPPREKIVGPLPELTGDDNPPKFKPFAFKDYSYRKLNKLP 334

BLAST of CSPI07G15650 vs. TAIR 10
Match: AT5G08640.2 (flavonol synthase 1 )

HSP 1 Score: 184.9 bits (468), Expect = 1.1e-46
Identity = 98/292 (33.56%), Postives = 157/292 (53.77%), Query Frame = 0

Query: 37  IPVIDLATEDRDLLSKKILDVSQEFGFFQVINHGVSKALVEETMRVSREFHAMSSEDKEM 96
           IPV+DL+  D + + + ++  S+E+G FQV+NHG+   L+     V R+F  + S +KE 
Sbjct: 43  IPVVDLSDPDEESVRRAVVKASEEWGLFQVVNHGIPTELIRRLQDVGRKFFELPSSEKES 102

Query: 97  ECSKDPNKSCLIYSSTPNYEKEELHLWKDSLRLICYPNLNKNVQFWPQNPPKFREVMEAY 156
               + +K    Y +    + E    W D L    +P    N +FWP+NPP++REV E Y
Sbjct: 103 VAKPEDSKDIEGYGTKLQKDPEGKKAWVDHLFHRIWPPSCVNYRFWPKNPPEYREVNEEY 162

Query: 157 LVVMKKFSLEILEVLSEGLGLEKEYFSGGMSNN-PEFL--VHHYPPCPNPNLTLGLNKHA 216
            V +KK S  +L +LS+GLGL+++    G+     E++  +++YPPCP P+L LG+  H 
Sbjct: 163 AVHVKKLSETLLGILSDGLGLKRDALKEGLGGEMAEYMMKINYYPPCPRPDLALGVPAHT 222

Query: 217 DPTVITILF-QDVNGLQFLKDGEWIDVDPLPDAFLVNLGYVFEVISNGKMKAAEHRVVTN 276
           D + IT+L   +V GLQ  KD  W D + +P A +V++G     +SNG+ K   HR   +
Sbjct: 223 DLSGITLLVPNEVPGLQVFKDDHWFDAEYIPSAVIVHIGDQILRLSNGRYKNVLHRTTVD 282

Query: 277 TTTSRQSLAYGVYPENNMTIEPSKCFINEANPPHYRTLEFKDFVNKLRSKHP 325
              +R S    + P     + P      + NPP ++   FKD+  +  +K P
Sbjct: 283 KEKTRMSWPVFLEPPREKIVGPLPELTGDDNPPKFKPFAFKDYSYRKLNKLP 334