CSPI07G14680 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI07G14680
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionExopolygalacturonase-like
LocationChr7: 13172638 .. 13174386 (-)
RNA-Seq ExpressionCSPI07G14680
SyntenyCSPI07G14680
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACAAGAAATCTTAGCCTCATTCAAATCCTTCTCTTAGTATTTGCTTGGCAATGTTGTGCTGTCGTTTTCGATCACAGCGCCGGCACTCAAAATCGTCTCAAGGGGATCGTACCAACAACAAATGAGCAGTTTCTTCGTCCTGCAGAAGCACCTCGACGTCTTGGCTTCAGTACTCTTTCCAACATTGGCAGCACGGTGAATGACATTAAGGCAAATGTTGATCTAAATGAAAATGGTGTGTCAGTTTTTGATGTTACAAAACATGGAGCTAAAGCTAATGGAAAAACTGATGATGCACAGGTGAGAAATATCAACACACACTAACTTCAAACCTTTTAATTTGAGTTAATTATCTTTAAATTGATGACTATAGGAAATTGTATATATTTTTTGCTATTAAATTCTACCGTTATATTATATTTTGGTGGATATTGATGGTACAATTATTTCAACATTTTTTTGGCAAAAAGAGTTATAATTATTTTTTTTATTTTCCATTTCGAAGGCATTCATGACAACATGGATTGCAGCATGCCGGAACACAGTCGGTCCGGCAAAGTTCTTGATCCCACAAGGCACATTTCTGGTGGGTCCGGTGACTTTCGCTGGTCCTTGCAAAAGCTTTCCGATCACCCTCGAAAATCAAGGAACTGTAAAGGCCACCACCGATATTAGTGCATATTCTTCTCCAGAATGGTTCTCCATTGAAGATATTACTGGTTTCATCCTCACCGGCTCCGGCGTCTTTGACGGCCAAGGCCTCTCTGTTTGGTCTTACAACGACTGCAAGAAAAACAACTTATGCCAACTTCTTCCAATCGTAACTATATATTTTTGCCTTTAATTTAAATTACAAGTGTTTTACACTCTAAACTTTTTGATTTGTGTCTAACACGTATCTAAAAAAAACTTGTGATAGACCAAACATATTTAAAAATGAAAATTTATTTTAAATTACAAAAACTATTAAAATTGTTTAGAAAATATGTAGCAAAATTTCATATATCCTGTTGATAAGAATTTGAAATTTTACTTTAAATACTTTTATTTATTATGTTATATTCGAAAATGTCTTTTCAACTATGGTGATTAATTCTTTGATTTTGTTTTTTTTTTTTTGCAGTCGATTAAATTCATGAGATTAAATCACACGATTGTTGATGGGCTCACATCGATAAACAGCATGGGCTTTCACACGTCTGTATTCTACTGCTACAATTTCACTGCTACTAACATGAAAATTATAGCTCCACATAATAGTCCCAACACCGATGGAATGCACCTTAGCACCTCAAAATTGGTCACCATTGCCAATAGTGTCATTGGCACAGGTGATGATTGTGTCTCCATTGGTCACAGTACAGAGAGTATCACTGTCACCAATGTCACTTGCGGCCCTGGACATGGCCTTAGGTAAATAGTGCACTTTTTTATTAAAAAAGAAGTTAAAGATTAAATAGACAAAGGAAAACACCTAAAAGCTTTCCATTATGCATCTTTTTCAGTGTGGGCAGCTTAGGCAAGTACTCGAAAGAAAAGGGTGTCTATGACGTTCTAGTAAAGAACTGCACCATTTTCAATGCCACCAATGGTGCCAGAATCAAGACTTGGGCTAGCCCTATCTCGGGGTTAGCCTCGAGAATTATCTTTGAAGACATTGTAATGTACAACGTAAAAAATCCTATAATTATCGATCAAACCTACGGCACTAAGAAGAAGAAGGTAAATAATAACTATAAAAACTAA

mRNA sequence

ATGACAAGAAATCTTAGCCTCATTCAAATCCTTCTCTTAGTATTTGCTTGGCAATGTTGTGCTGTCGTTTTCGATCACAGCGCCGGCACTCAAAATCGTCTCAAGGGGATCGTACCAACAACAAATGAGCAGTTTCTTCGTCCTGCAGAAGCACCTCGACGTCTTGGCTTCAGTACTCTTTCCAACATTGGCAGCACGGTGAATGACATTAAGGCAAATGTTGATCTAAATGAAAATGGTGTGTCAGTTTTTGATGTTACAAAACATGGAGCTAAAGCTAATGGAAAAACTGATGATGCACAGGCATTCATGACAACATGGATTGCAGCATGCCGGAACACAGTCGGTCCGGCAAAGTTCTTGATCCCACAAGGCACATTTCTGGTGGGTCCGGTGACTTTCGCTGGTCCTTGCAAAAGCTTTCCGATCACCCTCGAAAATCAAGGAACTGTAAAGGCCACCACCGATATTAGTGCATATTCTTCTCCAGAATGGTTCTCCATTGAAGATATTACTGGTTTCATCCTCACCGGCTCCGGCGTCTTTGACGGCCAAGGCCTCTCTGTTTGGTCTTACAACGACTGCAAGAAAAACAACTTATGCCAACTTCTTCCAATCTCGATTAAATTCATGAGATTAAATCACACGATTGTTGATGGGCTCACATCGATAAACAGCATGGGCTTTCACACGTCTGTATTCTACTGCTACAATTTCACTGCTACTAACATGAAAATTATAGCTCCACATAATAGTCCCAACACCGATGGAATGCACCTTAGCACCTCAAAATTGGTCACCATTGCCAATAGTGTCATTGGCACAGGTGATGATTGTGTCTCCATTGGTCACAGTACAGAGAGTATCACTGTCACCAATGTCACTTGCGGCCCTGGACATGGCCTTAGTGTGGGCAGCTTAGGCAAGTACTCGAAAGAAAAGGGTGTCTATGACGTTCTAGTAAAGAACTGCACCATTTTCAATGCCACCAATGGTGCCAGAATCAAGACTTGGGCTAGCCCTATCTCGGGGTTAGCCTCGAGAATTATCTTTGAAGACATTGTAATGTACAACGTAAAAAATCCTATAATTATCGATCAAACCTACGGCACTAAGAAGAAGAAGGTAAATAATAACTATAAAAACTAA

Coding sequence (CDS)

ATGACAAGAAATCTTAGCCTCATTCAAATCCTTCTCTTAGTATTTGCTTGGCAATGTTGTGCTGTCGTTTTCGATCACAGCGCCGGCACTCAAAATCGTCTCAAGGGGATCGTACCAACAACAAATGAGCAGTTTCTTCGTCCTGCAGAAGCACCTCGACGTCTTGGCTTCAGTACTCTTTCCAACATTGGCAGCACGGTGAATGACATTAAGGCAAATGTTGATCTAAATGAAAATGGTGTGTCAGTTTTTGATGTTACAAAACATGGAGCTAAAGCTAATGGAAAAACTGATGATGCACAGGCATTCATGACAACATGGATTGCAGCATGCCGGAACACAGTCGGTCCGGCAAAGTTCTTGATCCCACAAGGCACATTTCTGGTGGGTCCGGTGACTTTCGCTGGTCCTTGCAAAAGCTTTCCGATCACCCTCGAAAATCAAGGAACTGTAAAGGCCACCACCGATATTAGTGCATATTCTTCTCCAGAATGGTTCTCCATTGAAGATATTACTGGTTTCATCCTCACCGGCTCCGGCGTCTTTGACGGCCAAGGCCTCTCTGTTTGGTCTTACAACGACTGCAAGAAAAACAACTTATGCCAACTTCTTCCAATCTCGATTAAATTCATGAGATTAAATCACACGATTGTTGATGGGCTCACATCGATAAACAGCATGGGCTTTCACACGTCTGTATTCTACTGCTACAATTTCACTGCTACTAACATGAAAATTATAGCTCCACATAATAGTCCCAACACCGATGGAATGCACCTTAGCACCTCAAAATTGGTCACCATTGCCAATAGTGTCATTGGCACAGGTGATGATTGTGTCTCCATTGGTCACAGTACAGAGAGTATCACTGTCACCAATGTCACTTGCGGCCCTGGACATGGCCTTAGTGTGGGCAGCTTAGGCAAGTACTCGAAAGAAAAGGGTGTCTATGACGTTCTAGTAAAGAACTGCACCATTTTCAATGCCACCAATGGTGCCAGAATCAAGACTTGGGCTAGCCCTATCTCGGGGTTAGCCTCGAGAATTATCTTTGAAGACATTGTAATGTACAACGTAAAAAATCCTATAATTATCGATCAAACCTACGGCACTAAGAAGAAGAAGGTAAATAATAACTATAAAAACTAA

Protein sequence

MTRNLSLIQILLLVFAWQCCAVVFDHSAGTQNRLKGIVPTTNEQFLRPAEAPRRLGFSTLSNIGSTVNDIKANVDLNENGVSVFDVTKHGAKANGKTDDAQAFMTTWIAACRNTVGPAKFLIPQGTFLVGPVTFAGPCKSFPITLENQGTVKATTDISAYSSPEWFSIEDITGFILTGSGVFDGQGLSVWSYNDCKKNNLCQLLPISIKFMRLNHTIVDGLTSINSMGFHTSVFYCYNFTATNMKIIAPHNSPNTDGMHLSTSKLVTIANSVIGTGDDCVSIGHSTESITVTNVTCGPGHGLSVGSLGKYSKEKGVYDVLVKNCTIFNATNGARIKTWASPISGLASRIIFEDIVMYNVKNPIIIDQTYGTKKKKVNNNYKN*
Homology
BLAST of CSPI07G14680 vs. ExPASy Swiss-Prot
Match: P49062 (Exopolygalacturonase clone GBGE184 OS=Arabidopsis thaliana OX=3702 GN=PGA3 PE=2 SV=1)

HSP 1 Score: 291.2 bits (744), Expect = 1.7e-77
Identity = 143/296 (48.31%), Postives = 192/296 (64.86%), Query Frame = 0

Query: 77  NENGVSVFDVTKHGAKANGKTDDAQAFMTTWIAACRNTVGPAKFLIPQGTFLVGPVTFAG 136
           N    +V+D+TK GA  +G T+  +AF+ TWI  C + V PA  L+P+GTFL GPV FAG
Sbjct: 45  NSGNPTVYDITKFGAVGDGSTNTFKAFLNTWIQVCDSPV-PATLLVPKGTFLAGPVIFAG 104

Query: 137 PCKSFPITLENQGTVKATTDISAYSSPEWFSIEDITGFILTGSGVFDGQGLSVWSYNDCK 196
           PCKS  +T+   GT+ ATT  S Y++PEWF  E +   +LTG+G F G+G +VW  + C 
Sbjct: 105 PCKS-KVTVNVIGTIIATT--SGYATPEWFLFERVDNLVLTGTGTFHGKGEAVWKADGCG 164

Query: 197 KNNLCQLLPISIKFMRLNHTIVDGLTSINSMGFHTSVFYCYNFTATNMKIIAPHNSPNTD 256
           K   C L P S+KF  + +  ++G++S+N+  FH  +    N    N+K+ AP  SPNTD
Sbjct: 165 KKVQCNLPPTSLKFRNMKNVEINGISSVNAKAFHMFLVKTENVNIQNIKLTAPAESPNTD 224

Query: 257 GMHLSTSKLVTIANSVIGTGDDCVSIGHSTESITVTNVTCGPGHGLSVGSLGKYSKEKGV 316
           G+HLS +  V+I +S I TGDDCVS+G  + ++TV  V CGPGHGLSVGSLGKY  E+ V
Sbjct: 225 GIHLSNADNVSILDSTIATGDDCVSVGRGSNNVTVERVICGPGHGLSVGSLGKYKNEEDV 284

Query: 317 YDVLVKNCTIFNATNGARIKTWASPISGLASRIIFEDIVMYNVKNPIIIDQTYGTK 373
             + V NCT+    NG RIKTW       A  I FE+I+M +VKNPIIIDQ YG++
Sbjct: 285 SGIHVNNCTMIETDNGLRIKTWGGSDPSKAVDIKFENIIMQSVKNPIIIDQNYGSR 336

BLAST of CSPI07G14680 vs. ExPASy Swiss-Prot
Match: Q39786 (Polygalacturonase OS=Gossypium hirsutum OX=3635 GN=G9 PE=2 SV=1)

HSP 1 Score: 268.5 bits (685), Expect = 1.2e-70
Identity = 131/284 (46.13%), Postives = 179/284 (63.03%), Query Frame = 0

Query: 86  VTKHGAKANGKTDDAQAFMTTWIAACRNTVGPAKFLIPQGTFLVGPVTFAGPCKSFPITL 145
           V K GAKA+GKTD ++ F+  W  AC  +V P+  +IP+GT+L+  V   GPCK+ PI +
Sbjct: 32  VAKFGAKADGKTDLSKPFLDAWKEACA-SVTPSTVVIPKGTYLLSKVNLEGPCKA-PIEI 91

Query: 146 ENQGTVKATTDISAYSSPEWFSIEDITGFILTGSGVFDGQGLSVWSYNDCKKNNLCQLLP 205
             QGT++A  D SA+  P W     +  F + G G+FDGQG   +  N C+       LP
Sbjct: 92  NVQGTIQAPADPSAFKDPNWVRFYSVENFKMFGGGIFDGQGSIAYEKNTCENREFRSKLP 151

Query: 206 ISIKFMRLNHTIVDGLTSINSMGFHTSVFYCYNFTATNMKIIAPHNSPNTDGMHLSTSKL 265
           ++I+F  L + ++  +TS +S  FH +VF C N T   +KI AP  SPNTDG+H+  S+ 
Sbjct: 152 VNIRFDFLTNALIQDITSKDSKLFHINVFACKNITLERLKIEAPDESPNTDGIHMGKSEG 211

Query: 266 VTIANSVIGTGDDCVSIGHSTESITVTNVTCGPGHGLSVGSLGKYSKEKGVYDVLVKNCT 325
           V I  S I TGDDC+SIG  T+++ +  +TCGPGHG+S+GSLGK+  E+ V  + + NCT
Sbjct: 212 VNIIASDIKTGDDCISIGDGTKNMVIKEITCGPGHGISIGSLGKFQNEEPVEGIKISNCT 271

Query: 326 IFNATNGARIKTWASPISGLASRIIFEDIVMYNVKNPIIIDQTY 370
           I N +NGARIKTW     G  S I FEDI M NV +PI+IDQ Y
Sbjct: 272 ITNTSNGARIKTWPGEHGGAVSEIHFEDITMNNVSSPILIDQQY 313

BLAST of CSPI07G14680 vs. ExPASy Swiss-Prot
Match: Q39766 (Polygalacturonase OS=Gossypium barbadense OX=3634 GN=G9 PE=2 SV=1)

HSP 1 Score: 267.3 bits (682), Expect = 2.6e-70
Identity = 130/284 (45.77%), Postives = 179/284 (63.03%), Query Frame = 0

Query: 86  VTKHGAKANGKTDDAQAFMTTWIAACRNTVGPAKFLIPQGTFLVGPVTFAGPCKSFPITL 145
           V K GAKA+GKTD ++ F+  W  AC  +V P+  +IP+GT+L+  V   GPCK+ PI +
Sbjct: 32  VAKFGAKADGKTDLSKPFLDAWKEACA-SVTPSTVVIPKGTYLLSKVNLEGPCKA-PIEI 91

Query: 146 ENQGTVKATTDISAYSSPEWFSIEDITGFILTGSGVFDGQGLSVWSYNDCKKNNLCQLLP 205
             QGT++A  D SA+  P W     +  F + G G+FDGQG   +  N C+       LP
Sbjct: 92  NVQGTIQAPADPSAFKDPNWVRFYSVENFKMFGGGIFDGQGSIAYEKNTCENREFRSKLP 151

Query: 206 ISIKFMRLNHTIVDGLTSINSMGFHTSVFYCYNFTATNMKIIAPHNSPNTDGMHLSTSKL 265
           ++I+F  + + ++  +TS +S  FH +VF C N T   +KI AP  SPNTDG+H+  S+ 
Sbjct: 152 VNIRFDFVTNALIQDITSKDSKLFHINVFACKNITLERLKIEAPDESPNTDGIHMGKSEG 211

Query: 266 VTIANSVIGTGDDCVSIGHSTESITVTNVTCGPGHGLSVGSLGKYSKEKGVYDVLVKNCT 325
           V I  S I TGDDC+SIG  T+++ +  +TCGPGHG+S+GSLGK+  E+ V  + + NCT
Sbjct: 212 VNIIASDIKTGDDCISIGDGTKNMVIKEITCGPGHGISIGSLGKFQNEEPVEGIKISNCT 271

Query: 326 IFNATNGARIKTWASPISGLASRIIFEDIVMYNVKNPIIIDQTY 370
           I N +NGARIKTW     G  S I FEDI M NV +PI+IDQ Y
Sbjct: 272 ITNTSNGARIKTWPGEHGGAVSEIHFEDITMNNVSSPILIDQQY 313

BLAST of CSPI07G14680 vs. ExPASy Swiss-Prot
Match: P35339 (Exopolygalacturonase OS=Zea mays OX=4577 GN=PG2C PE=2 SV=1)

HSP 1 Score: 265.4 bits (677), Expect = 1.0e-69
Identity = 135/292 (46.23%), Postives = 181/292 (61.99%), Query Frame = 0

Query: 84  FDVTKHGAKANGKTDDAQAFMTTWIAACRNTVGPAKFLIPQGTFLVGPVTFAGPCKSFPI 143
           FD+TK GA  NGKTD  +A    W +AC  T G    LIP+G FLVGP+ F GPCK   +
Sbjct: 41  FDITKLGASGNGKTDSTKAVQEAWASACGGT-GKQTILIPKGDFLVGPLNFTGPCKG-DV 100

Query: 144 TLENQGTVKATTDISAYSS-PEWFSIEDITGFILTGSGVFDGQGLSVWSYNDCKKNNLCQ 203
           T++  G + ATTD+S Y     W  I  +   ++TG G  DGQG +VWS N C K   C+
Sbjct: 101 TIQVNGNLLATTDLSQYKDHGNWIEILRVDNLVITGKGKLDGQGPAVWSKNSCVKKYDCK 160

Query: 204 LLPISIKFMRLNHTIVDGLTSINSMGFHTSVFYCYNFTATNMKIIAPHNSPNTDGMHLST 263
           +LP S+    +N+  V G+T +NS  FH +++ C +    ++ + AP +SPNTDG+H+  
Sbjct: 161 ILPNSLVMDFVNNGEVSGITLLNSKFFHMNMYKCKDMLIKDVNVTAPGDSPNTDGIHMGD 220

Query: 264 SKLVTIANSVIGTGDDCVSIGHSTESITVTNVTCGPGHGLSVGSLGKYSKEKGVYDVLVK 323
           S  VTI N+VIG GDDC+SIG  T  + +T VTCGPGHG+S+GSLG+Y  EK V D+ VK
Sbjct: 221 SSGVTITNTVIGVGDDCISIGPGTSKVNITGVTCGPGHGISIGSLGRYKDEKDVTDINVK 280

Query: 324 NCTIFNATNGARIKTWASPISGL-ASRIIFEDIVMYNVKNPIIIDQTYGTKK 374
           +CT+    NG RIK +    S L AS+I +E+I M +   PIIID  Y   K
Sbjct: 281 DCTLKKTANGVRIKAYEDAASVLTASKIHYENIKMEDSGYPIIIDMKYCPNK 330

BLAST of CSPI07G14680 vs. ExPASy Swiss-Prot
Match: Q6H9K0 (Exopolygalacturonase (Fragment) OS=Platanus acerifolia OX=140101 GN=plaa2 PE=1 SV=1)

HSP 1 Score: 264.6 bits (675), Expect = 1.7e-69
Identity = 129/288 (44.79%), Postives = 181/288 (62.85%), Query Frame = 0

Query: 82  SVFDVTKHGAKANGKTDDAQAFMTTWIAACRNTVGPAKFLIPQGTFLVGPVTFAGPCKSF 141
           SVF+V  +GAK  G  D +QA M  W AAC +  GP+  LIP+G + +G V   GPCK  
Sbjct: 8   SVFNVNDYGAKGAG--DISQAVMKAWKAACASQ-GPSTVLIPKGNYNMGEVAMQGPCKGS 67

Query: 142 PITLENQGTVKATTDISAYSSPEWFSIEDITGFILTGSGVFDGQGLSVWSYNDCKKNNLC 201
            I  +  G VKA  D S + S  W S   I G  ++G+G  DGQG + W+ N+C KN  C
Sbjct: 68  KIGFQIDGVVKAPADPSKFKSDGWVSFYRIDGLTVSGTGTLDGQGQTAWAKNNCDKNPNC 127

Query: 202 QLLPISIKFMRLNHTIVDGLTSINSMGFHTSVFYCYNFTATNMKIIAPHNSPNTDGMHLS 261
           +   ++++F  L H +V  +TS+NS  FH +V  C + T  ++ + AP  S NTDG+H+ 
Sbjct: 128 KHAAMNLRFDFLKHAMVRDITSLNSKMFHINVLECEDITFQHVTVTAPGTSINTDGIHVG 187

Query: 262 TSKLVTIANSVIGTGDDCVSIGHSTESITVTNVTCGPGHGLSVGSLGKYSKEKGVYDVLV 321
            SK VTI N+ I TGDDC+SIG  ++++T+T V CGPGHG+S+GSLG+Y+ EK V  + V
Sbjct: 188 ISKGVTITNTKIATGDDCISIGPGSQNVTITQVNCGPGHGISIGSLGRYNNEKEVRGITV 247

Query: 322 KNCTIFNATNGARIKTWASPISGLASRIIFEDIVMYNVKNPIIIDQTY 370
           K CT     NG R+KTW +   G A+ + F+D+ M NV+NP+I+DQ Y
Sbjct: 248 KGCTFSGTMNGVRVKTWPNSPPGAATDLTFQDLTMNNVQNPVILDQEY 292

BLAST of CSPI07G14680 vs. ExPASy TrEMBL
Match: A0A5A7U4W1 (Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G00950 PE=3 SV=1)

HSP 1 Score: 674.5 bits (1739), Expect = 2.7e-190
Identity = 338/382 (88.48%), Postives = 351/382 (91.88%), Query Frame = 0

Query: 1   MTRNLSL-IQILLLVFAWQCCAVV---FDHSAGTQNRLKGIVPTTNEQFLRPAEAPRRLG 60
           MTRNLSL IQILLLVFA QC A V    D   GT+N LK  VPT NEQFLRPA APRRLG
Sbjct: 1   MTRNLSLVIQILLLVFASQCSAKVAATLDDVTGTENLLKVTVPTVNEQFLRPAGAPRRLG 60

Query: 61  FSTLSNIGSTVNDIKANVDLNENGVSVFDVTKHGAKANGKTDDAQAFMTTWIAACRNTVG 120
             TL +IGSTVNDI+AN+ LNENG SVFDVTKHGAKA+G+TDDAQAFMTTWIAACRNTVG
Sbjct: 61  IGTLPDIGSTVNDIRANIVLNENGGSVFDVTKHGAKADGETDDAQAFMTTWIAACRNTVG 120

Query: 121 PAKFLIPQGTFLVGPVTFAGPCKSFPITLENQGTVKATTDISAYSSPEWFSIEDITGFIL 180
           PAKFLIPQGT+LVGPVTFAGPCKSFPITLENQGTVKATTDIS YSSPEWFSIEDITGFIL
Sbjct: 121 PAKFLIPQGTYLVGPVTFAGPCKSFPITLENQGTVKATTDISEYSSPEWFSIEDITGFIL 180

Query: 181 TGSGVFDGQGLSVWSYNDCKKNNLCQLLPISIKFMRLNHTIVDGLTSINSMGFHTSVFYC 240
           TGSGVFDGQG++VW YNDCKKN  CQLLPISIKF RLNHTIVDGLTS+NSMGFHTSVFYC
Sbjct: 181 TGSGVFDGQGVTVWPYNDCKKNRFCQLLPISIKFSRLNHTIVDGLTSVNSMGFHTSVFYC 240

Query: 241 YNFTATNMKIIAPHNSPNTDGMHLSTSKLVTIANSVIGTGDDCVSIGHSTESITVTNVTC 300
           YNFTATNMKIIAPHNSPNTDGMHLSTSKLVTI NSVIGTGDDCVSIGHSTE+I VTNVTC
Sbjct: 241 YNFTATNMKIIAPHNSPNTDGMHLSTSKLVTITNSVIGTGDDCVSIGHSTENIVVTNVTC 300

Query: 301 GPGHGLSVGSLGKYSKEKGVYDVLVKNCTIFNATNGARIKTWASPISGLASRIIFEDIVM 360
           GPGHGLSVGSLGKYSKEK VY+VLVKNCTIFNATNGARIKTWAS ISGLAS IIFEDIVM
Sbjct: 301 GPGHGLSVGSLGKYSKEKSVYNVLVKNCTIFNATNGARIKTWASRISGLASGIIFEDIVM 360

Query: 361 YNVKNPIIIDQTYGTKKKKVNN 379
           YNVKNPIIIDQTYGTKKKKV+N
Sbjct: 361 YNVKNPIIIDQTYGTKKKKVSN 382

BLAST of CSPI07G14680 vs. ExPASy TrEMBL
Match: A0A5A7TQ89 (Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold36913G00010 PE=3 SV=1)

HSP 1 Score: 673.7 bits (1737), Expect = 4.5e-190
Identity = 337/382 (88.22%), Postives = 350/382 (91.62%), Query Frame = 0

Query: 1   MTRNLSL-IQILLLVFAWQCC---AVVFDHSAGTQNRLKGIVPTTNEQFLRPAEAPRRLG 60
           MTRNLSL IQILLLVFA QC    A   D   GT+N LK  VPT NEQFLRPA APRRLG
Sbjct: 1   MTRNLSLVIQILLLVFASQCSAKDAATLDDVTGTENLLKVTVPTVNEQFLRPAGAPRRLG 60

Query: 61  FSTLSNIGSTVNDIKANVDLNENGVSVFDVTKHGAKANGKTDDAQAFMTTWIAACRNTVG 120
             TL +IGSTVNDI+AN+ LNENG SVFDVTKHGAKA+G+TDDAQAFMTTWIAACRNTVG
Sbjct: 61  IGTLPDIGSTVNDIRANIVLNENGGSVFDVTKHGAKADGETDDAQAFMTTWIAACRNTVG 120

Query: 121 PAKFLIPQGTFLVGPVTFAGPCKSFPITLENQGTVKATTDISAYSSPEWFSIEDITGFIL 180
           PAKFLIPQGT+LVGPVTFAGPCKSFPITLENQGTVKATTDIS YSSPEWFSIEDITGFIL
Sbjct: 121 PAKFLIPQGTYLVGPVTFAGPCKSFPITLENQGTVKATTDISEYSSPEWFSIEDITGFIL 180

Query: 181 TGSGVFDGQGLSVWSYNDCKKNNLCQLLPISIKFMRLNHTIVDGLTSINSMGFHTSVFYC 240
           TGSGVFDGQG++VW YNDCKKN  CQLLPISIKF RLNHTIVDGLTS+NSMGFHTSVFYC
Sbjct: 181 TGSGVFDGQGVTVWPYNDCKKNRFCQLLPISIKFSRLNHTIVDGLTSVNSMGFHTSVFYC 240

Query: 241 YNFTATNMKIIAPHNSPNTDGMHLSTSKLVTIANSVIGTGDDCVSIGHSTESITVTNVTC 300
           YNFTATNMKIIAPHNSPNTDGMHLSTSKLVTI NSVIGTGDDCVSIGHSTE+I VTNVTC
Sbjct: 241 YNFTATNMKIIAPHNSPNTDGMHLSTSKLVTITNSVIGTGDDCVSIGHSTENIVVTNVTC 300

Query: 301 GPGHGLSVGSLGKYSKEKGVYDVLVKNCTIFNATNGARIKTWASPISGLASRIIFEDIVM 360
           GPGHGLSVGSLGKYSKEK VY+VLVKNCTIFNATNGARIKTWAS ISGLAS IIFEDIVM
Sbjct: 301 GPGHGLSVGSLGKYSKEKSVYNVLVKNCTIFNATNGARIKTWASRISGLASGIIFEDIVM 360

Query: 361 YNVKNPIIIDQTYGTKKKKVNN 379
           YNVKNPIIIDQTYGTKKKKV+N
Sbjct: 361 YNVKNPIIIDQTYGTKKKKVSN 382

BLAST of CSPI07G14680 vs. ExPASy TrEMBL
Match: A0A0A0KMA6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G222970 PE=3 SV=1)

HSP 1 Score: 662.5 bits (1708), Expect = 1.0e-186
Identity = 324/346 (93.64%), Postives = 334/346 (96.53%), Query Frame = 0

Query: 33  RLKGIVPTTNEQFLRPAEAPRRLGFSTLSNIGSTVNDIKANVDLNENGVSVFDVTKHGAK 92
           RL GIVPTTN+QFLRPAEAPR LGF+TL +IG TVNDI ANVDLNENG SVFDVTKHGAK
Sbjct: 5   RLYGIVPTTNKQFLRPAEAPRHLGFNTLPDIGGTVNDINANVDLNENGGSVFDVTKHGAK 64

Query: 93  ANGKTDDAQAFMTTWIAACRNTVGPAKFLIPQGTFLVGPVTFAGPCKSFPITLENQGTVK 152
           A+GKTDDAQAFMTTWIAACRNTVGPAKFLIPQGTFLVGPVTFAGPCKS+PITLENQGTVK
Sbjct: 65  ADGKTDDAQAFMTTWIAACRNTVGPAKFLIPQGTFLVGPVTFAGPCKSYPITLENQGTVK 124

Query: 153 ATTDISAYSSPEWFSIEDITGFILTGSGVFDGQGLSVWSYNDCKKNNLCQLLPISIKFMR 212
           ATTDISAYSSPEWFS+EDITGFILTGSGVFDGQGLSVW YNDCKKNNLCQLLPISIKF R
Sbjct: 125 ATTDISAYSSPEWFSLEDITGFILTGSGVFDGQGLSVWPYNDCKKNNLCQLLPISIKFTR 184

Query: 213 LNHTIVDGLTSINSMGFHTSVFYCYNFTATNMKIIAPHNSPNTDGMHLSTSKLVTIANSV 272
           LNHTIVDGLTSINSMGFHTSVFYCYNFTATNMKIIAPHNSPNTDGMHLSTSKLVTIANS+
Sbjct: 185 LNHTIVDGLTSINSMGFHTSVFYCYNFTATNMKIIAPHNSPNTDGMHLSTSKLVTIANSI 244

Query: 273 IGTGDDCVSIGHSTESITVTNVTCGPGHGLSVGSLGKYSKEKGVYDVLVKNCTIFNATNG 332
           IGTGDDCVSIGHSTE+ITVTNVTCGPGHGL   SLGKYSKEKGVYDVLVKNCTIFNATNG
Sbjct: 245 IGTGDDCVSIGHSTENITVTNVTCGPGHGL---SLGKYSKEKGVYDVLVKNCTIFNATNG 304

Query: 333 ARIKTWASPISGLASRIIFEDIVMYNVKNPIIIDQTYGTKKKKVNN 379
           ARIKTWASP+SGLASRIIFEDIVMYNVKNPIIIDQTYGTKKKK +N
Sbjct: 305 ARIKTWASPVSGLASRIIFEDIVMYNVKNPIIIDQTYGTKKKKESN 347

BLAST of CSPI07G14680 vs. ExPASy TrEMBL
Match: A0A0A0K5I2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G398220 PE=3 SV=1)

HSP 1 Score: 597.0 bits (1538), Expect = 5.4e-167
Identity = 303/364 (83.24%), Postives = 317/364 (87.09%), Query Frame = 0

Query: 1   MTRNLSLIQILLLVFAWQCC---AVVFDHSAGTQNRLKGIVPTTNEQFLRPAEAPRRLGF 60
           MTRN SLIQILLLVFAW+CC   A +F  +  TQ  L GIVP  NEQFLRPAEAP  LGF
Sbjct: 1   MTRNFSLIQILLLVFAWECCARAAAIFIDTINTQILLNGIVPKVNEQFLRPAEAPWHLGF 60

Query: 61  STLSNI-GSTVNDIKANVDLNENGVSVFDVTKHGAKANGKTDDAQAFMTTWIAACRNTVG 120
           STL NI G TVNDIK NVDLNENG  VFDVTKHGAKA+G+TDDAQAFMTTWIAACRNTVG
Sbjct: 61  STLPNIGGGTVNDIKVNVDLNENGGLVFDVTKHGAKADGETDDAQAFMTTWIAACRNTVG 120

Query: 121 PAKFLIPQGTFLVGPVTFAGPCKSFPITLENQGTVKATTDISAYSSPEWFSIEDITGFIL 180
           PAKFLIPQ  FLVGPVTFAGPCKSFPITLENQGTVKATTDISAYSS EWFSIED+TGFIL
Sbjct: 121 PAKFLIPQDIFLVGPVTFAGPCKSFPITLENQGTVKATTDISAYSSSEWFSIEDVTGFIL 180

Query: 181 TGSGVFDGQGLSVWSYNDCKKNNLCQLLPISIKFMRLNHTIVDGLTSINSMGFHTSVFYC 240
           TGSGVFDGQG+S                  SIKF RLNHTI+DGLTSINSMGFHTSVFYC
Sbjct: 181 TGSGVFDGQGVS------------------SIKFSRLNHTIIDGLTSINSMGFHTSVFYC 240

Query: 241 YNFTATNMKIIAPHNSPNTDGMHLSTSKLVTIANSVIGTGDDCVSIGHSTESITVTNVTC 300
           YNFTATNMKI APHNSPNTDGMHLSTSKLVTI+NS+IGTGDDCVSIGHSTE+I +TNVTC
Sbjct: 241 YNFTATNMKITAPHNSPNTDGMHLSTSKLVTISNSIIGTGDDCVSIGHSTENIIITNVTC 300

Query: 301 GPGHGLSVGSLGKYSKEKGVYDVLVKNCTIFNATNGARIKTWASPISGLASRIIFEDIVM 360
           GPGHGLSVGSLGKYSKEKGVYDVLVKNCTIFNATNGARIKT+ASPI GLASRI+FEDIVM
Sbjct: 301 GPGHGLSVGSLGKYSKEKGVYDVLVKNCTIFNATNGARIKTFASPIPGLASRIVFEDIVM 346

BLAST of CSPI07G14680 vs. ExPASy TrEMBL
Match: A0A1S3BRD3 (exopolygalacturonase-like OS=Cucumis melo OX=3656 GN=LOC103492867 PE=3 SV=1)

HSP 1 Score: 581.6 bits (1498), Expect = 2.3e-162
Identity = 291/376 (77.39%), Postives = 315/376 (83.78%), Query Frame = 0

Query: 1   MTRNLSLIQILLLVFAWQCCAVVFDHSAGTQNRLKGIVPTTNEQFLRPAEAPRRLGFSTL 60
           +TR++ L QILL VFAWQCC  V   +    N    IV T N+Q   P  AP  LG  TL
Sbjct: 3   ITRDIGLFQILLWVFAWQCCDKVSAITNDIANLNYEIVSTINQQLPSPVAAPGPLGIETL 62

Query: 61  SNIGSTVN-DIKANVDLNENGVSVFDVTKHGAKANGKTDDAQAFMTTWIAACRNTVGPAK 120
            ++ + VN DI  N DLN NG SVF VTKHGAKA+GKTDDAQAF+TTWI ACRNTVGPAK
Sbjct: 63  PDLDNMVNDDINTNNDLNGNGGSVFHVTKHGAKADGKTDDAQAFVTTWIEACRNTVGPAK 122

Query: 121 FLIPQGTFLVGPVTFAGPCKSFPITLENQGTVKATTDISAYSSPEWFSIEDITGFILTGS 180
            LIP+GT+LVGPVT AGPCKSFPITLENQGTVKATTDIS YSSPEWFSIEDITGFILTGS
Sbjct: 123 ILIPEGTYLVGPVTLAGPCKSFPITLENQGTVKATTDISQYSSPEWFSIEDITGFILTGS 182

Query: 181 GVFDGQGLSVWSYNDCKKNNLCQLLPISIKFMRLNHTIVDGLTSINSMGFHTSVFYCYNF 240
           GVFDGQG++ W YNDCK NN+CQLLPISIKF RLN TIVD LTS+NS GFH S+F CYNF
Sbjct: 183 GVFDGQGVAAWPYNDCKNNNICQLLPISIKFSRLNDTIVDRLTSLNSKGFHMSLFSCYNF 242

Query: 241 TATNMKIIAPHNSPNTDGMHLSTSKLVTIANSVIGTGDDCVSIGHSTESITVTNVTCGPG 300
           TATN+ IIAPH+SPNTDG+HLSTSKLV IANS+IGTGDDCVSIGHSTE ITVTNVTCGPG
Sbjct: 243 TATNINIIAPHDSPNTDGIHLSTSKLVNIANSIIGTGDDCVSIGHSTEKITVTNVTCGPG 302

Query: 301 HGLSVGSLGKYSKEKGVYDVLVKNCTIFNATNGARIKTWASPISGLASRIIFEDIVMYNV 360
           HGLSVGSLGKY +EK VYDVLVKNCTIFNATNGARIKT+ASPISG AS IIFEDIVMYNV
Sbjct: 303 HGLSVGSLGKYPEEKDVYDVLVKNCTIFNATNGARIKTFASPISGSASGIIFEDIVMYNV 362

Query: 361 KNPIIIDQTYGTKKKK 376
           K PIIIDQTY T + K
Sbjct: 363 KYPIIIDQTYDTNENK 378

BLAST of CSPI07G14680 vs. NCBI nr
Match: XP_031745094.1 (exopolygalacturonase-like [Cucumis sativus])

HSP 1 Score: 744.2 bits (1920), Expect = 5.6e-211
Identity = 364/378 (96.30%), Postives = 369/378 (97.62%), Query Frame = 0

Query: 1   MTRNLSLIQILLLVFAWQCCAVVFDHSAGTQNRLKGIVPTTNEQFLRPAEAPRRLGFSTL 60
           MTRNLSLIQILLLVFAWQCCAVVFD   GTQNRLKGIVPTTNEQFLRPAEAPRRLGFSTL
Sbjct: 1   MTRNLSLIQILLLVFAWQCCAVVFDDITGTQNRLKGIVPTTNEQFLRPAEAPRRLGFSTL 60

Query: 61  SNIGSTVNDIKANVDLNENGVSVFDVTKHGAKANGKTDDAQAFMTTWIAACRNTVGPAKF 120
            NIG TVNDIKANVDLN+NG SVFDVTKHGAKANGKTDDAQAFMTTWIAACRNTVGPAKF
Sbjct: 61  PNIGGTVNDIKANVDLNQNGGSVFDVTKHGAKANGKTDDAQAFMTTWIAACRNTVGPAKF 120

Query: 121 LIPQGTFLVGPVTFAGPCKSFPITLENQGTVKATTDISAYSSPEWFSIEDITGFILTGSG 180
           LIPQGTFLVGPVTFAGPCKSFPITLENQGTVKATTDISAYSSPEWFSIEDITGFILTGSG
Sbjct: 121 LIPQGTFLVGPVTFAGPCKSFPITLENQGTVKATTDISAYSSPEWFSIEDITGFILTGSG 180

Query: 181 VFDGQGLSVWSYNDCKKNNLCQLLPISIKFMRLNHTIVDGLTSINSMGFHTSVFYCYNFT 240
           VFDGQGLSVW YNDCKKNNLCQLLPISIKF RLNHTIVDGLTS+NSMGFHTSVFYCYNFT
Sbjct: 181 VFDGQGLSVWPYNDCKKNNLCQLLPISIKFTRLNHTIVDGLTSVNSMGFHTSVFYCYNFT 240

Query: 241 ATNMKIIAPHNSPNTDGMHLSTSKLVTIANSVIGTGDDCVSIGHSTESITVTNVTCGPGH 300
           ATNMKIIAPHNSPNTDGMHLSTSKLVTIANSVIGTGDDCVSIGHSTE+ITVTNVTCGPGH
Sbjct: 241 ATNMKIIAPHNSPNTDGMHLSTSKLVTIANSVIGTGDDCVSIGHSTENITVTNVTCGPGH 300

Query: 301 GLSVGSLGKYSKEKGVYDVLVKNCTIFNATNGARIKTWASPISGLASRIIFEDIVMYNVK 360
           GLSVGSLGKYSKEKGVYDVLVKNCTIFNATNGARIKTWASP+SGLASRIIFEDIVMYNVK
Sbjct: 301 GLSVGSLGKYSKEKGVYDVLVKNCTIFNATNGARIKTWASPVSGLASRIIFEDIVMYNVK 360

Query: 361 NPIIIDQTYGTKKKKVNN 379
           NPIIIDQTYGTKKKK +N
Sbjct: 361 NPIIIDQTYGTKKKKESN 378

BLAST of CSPI07G14680 vs. NCBI nr
Match: XP_031745095.1 (exopolygalacturonase-like [Cucumis sativus])

HSP 1 Score: 742.7 bits (1916), Expect = 1.6e-210
Identity = 363/375 (96.80%), Postives = 367/375 (97.87%), Query Frame = 0

Query: 1   MTRNLSLIQILLLVFAWQCCAVVFDHSAGTQNRLKGIVPTTNEQFLRPAEAPRRLGFSTL 60
           MTRNLSLIQILLLVFAWQCCAVVFD   GTQNRLKGIVPTTNEQFLRPAEAPRRLGFSTL
Sbjct: 1   MTRNLSLIQILLLVFAWQCCAVVFDDITGTQNRLKGIVPTTNEQFLRPAEAPRRLGFSTL 60

Query: 61  SNIGSTVNDIKANVDLNENGVSVFDVTKHGAKANGKTDDAQAFMTTWIAACRNTVGPAKF 120
            NIG TVNDIKANVDLN+NG SVFDVTKHGAKANGKTDDAQAFMTTWIAACRNTVGPAKF
Sbjct: 61  PNIGGTVNDIKANVDLNQNGGSVFDVTKHGAKANGKTDDAQAFMTTWIAACRNTVGPAKF 120

Query: 121 LIPQGTFLVGPVTFAGPCKSFPITLENQGTVKATTDISAYSSPEWFSIEDITGFILTGSG 180
           LIPQGTFLVGPVTFAGPCKSFPITLENQGTVKATTDISAYSSPEWFSIEDITGFILTGSG
Sbjct: 121 LIPQGTFLVGPVTFAGPCKSFPITLENQGTVKATTDISAYSSPEWFSIEDITGFILTGSG 180

Query: 181 VFDGQGLSVWSYNDCKKNNLCQLLPISIKFMRLNHTIVDGLTSINSMGFHTSVFYCYNFT 240
           VFDGQGLSVW YNDCKKNNLCQLLPISIKF RLNHTIVDGLTS+NSMGFHTSVFYCYNFT
Sbjct: 181 VFDGQGLSVWPYNDCKKNNLCQLLPISIKFTRLNHTIVDGLTSVNSMGFHTSVFYCYNFT 240

Query: 241 ATNMKIIAPHNSPNTDGMHLSTSKLVTIANSVIGTGDDCVSIGHSTESITVTNVTCGPGH 300
           ATNMKIIAPHNSPNTDGMHLSTSKLVTIANSVIGTGDDCVSIGHSTE+ITVTNVTCGPGH
Sbjct: 241 ATNMKIIAPHNSPNTDGMHLSTSKLVTIANSVIGTGDDCVSIGHSTENITVTNVTCGPGH 300

Query: 301 GLSVGSLGKYSKEKGVYDVLVKNCTIFNATNGARIKTWASPISGLASRIIFEDIVMYNVK 360
           GLSVGSLGKYSKEKGVYDVLVKNCTIFNATNGARIKTWASP+SGLASRIIFEDIVMYNVK
Sbjct: 301 GLSVGSLGKYSKEKGVYDVLVKNCTIFNATNGARIKTWASPVSGLASRIIFEDIVMYNVK 360

Query: 361 NPIIIDQTYGTKKKK 376
           NPIIIDQTYGTKKKK
Sbjct: 361 NPIIIDQTYGTKKKK 375

BLAST of CSPI07G14680 vs. NCBI nr
Match: XP_031741281.1 (exopolygalacturonase [Cucumis sativus])

HSP 1 Score: 714.5 bits (1843), Expect = 4.8e-202
Identity = 351/378 (92.86%), Postives = 361/378 (95.50%), Query Frame = 0

Query: 1   MTRNLSLIQILLLVFAWQCCAVVFDHSAGTQNRLKGIVPTTNEQFLRPAEAPRRLGFSTL 60
           MTRNLSLIQI+LL FAWQ CAV+F+   GTQN LKGIVPTTN+QFLRPAEAPR LGF+TL
Sbjct: 1   MTRNLSLIQIILLEFAWQSCAVIFNDITGTQNLLKGIVPTTNKQFLRPAEAPRHLGFNTL 60

Query: 61  SNIGSTVNDIKANVDLNENGVSVFDVTKHGAKANGKTDDAQAFMTTWIAACRNTVGPAKF 120
            +IG TVNDI ANVDLNENG SVFDVTKHGAKA+GKTDDAQAFMTTWIAACRNTVGPAKF
Sbjct: 61  PDIGGTVNDINANVDLNENGGSVFDVTKHGAKADGKTDDAQAFMTTWIAACRNTVGPAKF 120

Query: 121 LIPQGTFLVGPVTFAGPCKSFPITLENQGTVKATTDISAYSSPEWFSIEDITGFILTGSG 180
           LIPQGTFLVGPVTFAGPCKS+PITLENQGTVKATTDISAYSSPEWFSIEDITGFILTGSG
Sbjct: 121 LIPQGTFLVGPVTFAGPCKSYPITLENQGTVKATTDISAYSSPEWFSIEDITGFILTGSG 180

Query: 181 VFDGQGLSVWSYNDCKKNNLCQLLPISIKFMRLNHTIVDGLTSINSMGFHTSVFYCYNFT 240
           VFDGQGLSVW YNDCKKNNLCQLLPISIKF RLNHTIVDGLTSINSMGFHTSVFYCYNFT
Sbjct: 181 VFDGQGLSVWPYNDCKKNNLCQLLPISIKFTRLNHTIVDGLTSINSMGFHTSVFYCYNFT 240

Query: 241 ATNMKIIAPHNSPNTDGMHLSTSKLVTIANSVIGTGDDCVSIGHSTESITVTNVTCGPGH 300
           ATNMKIIAPHNSPNTDG HLSTSKLVTIANSVIGTGDDCVSIGHSTE+ITVTNVTCGPGH
Sbjct: 241 ATNMKIIAPHNSPNTDGTHLSTSKLVTIANSVIGTGDDCVSIGHSTENITVTNVTCGPGH 300

Query: 301 GLSVGSLGKYSKEKGVYDVLVKNCTIFNATNGARIKTWASPISGLASRIIFEDIVMYNVK 360
           GLSVGSLGKYSKEKGVYDVLVKNCTIFNATNGARIKTWAS ISGLASRIIFEDIVMYNVK
Sbjct: 301 GLSVGSLGKYSKEKGVYDVLVKNCTIFNATNGARIKTWASRISGLASRIIFEDIVMYNVK 360

Query: 361 NPIIIDQTYGTKKKKVNN 379
           NPIIIDQTY TKKKK +N
Sbjct: 361 NPIIIDQTYSTKKKKESN 378

BLAST of CSPI07G14680 vs. NCBI nr
Match: XP_031745103.1 (exopolygalacturonase-like [Cucumis sativus])

HSP 1 Score: 691.0 bits (1782), Expect = 5.7e-195
Identity = 335/365 (91.78%), Postives = 346/365 (94.79%), Query Frame = 0

Query: 14  VFAWQCCAVVFDHSAGTQNRLKGIVPTTNEQFLRPAEAPRRLGFSTLSNIGSTVNDIKAN 73
           VFA QCCAV+FD   GTQN LKGIVP  N+QFLRPAEAPR LGFSTL NIG TVNDIKAN
Sbjct: 62  VFACQCCAVIFDDVTGTQNLLKGIVPKANKQFLRPAEAPRHLGFSTLPNIGGTVNDIKAN 121

Query: 74  VDLNENGVSVFDVTKHGAKANGKTDDAQAFMTTWIAACRNTVGPAKFLIPQGTFLVGPVT 133
           VDLNENG SVFDVTKHGAK +G+TDDA AFMTTWIAACRNTVGP KFLIPQGTFLVGPVT
Sbjct: 122 VDLNENGGSVFDVTKHGAKGDGETDDAHAFMTTWIAACRNTVGPTKFLIPQGTFLVGPVT 181

Query: 134 FAGPCKSFPITLENQGTVKATTDISAYSSPEWFSIEDITGFILTGSGVFDGQGLSVWSYN 193
           FAGPCKSFPITLENQGTVKATTDISAYSSPEWFS+EDITGFILTGSGVFDGQGLSVW+YN
Sbjct: 182 FAGPCKSFPITLENQGTVKATTDISAYSSPEWFSLEDITGFILTGSGVFDGQGLSVWAYN 241

Query: 194 DCKKNNLCQLLPISIKFMRLNHTIVDGLTSINSMGFHTSVFYCYNFTATNMKIIAPHNSP 253
           DCKKNNLCQ LPISIKF RLNHTIVDGLTSINSMGFHTSVFYCYNFTATNMKIIAPHNSP
Sbjct: 242 DCKKNNLCQPLPISIKFTRLNHTIVDGLTSINSMGFHTSVFYCYNFTATNMKIIAPHNSP 301

Query: 254 NTDGMHLSTSKLVTIANSVIGTGDDCVSIGHSTESITVTNVTCGPGHGLSVGSLGKYSKE 313
           NTDGMHLSTSKLVTIANS+IGTGDDCVSIGHSTE+IT+TNVTCGPGHGLSVGSLGKYSKE
Sbjct: 302 NTDGMHLSTSKLVTIANSIIGTGDDCVSIGHSTENITITNVTCGPGHGLSVGSLGKYSKE 361

Query: 314 KGVYDVLVKNCTIFNATNGARIKTWASPISGLASRIIFEDIVMYNVKNPIIIDQTYGTKK 373
           KGVYDVLVKNCTIFNATNGARIKTWASPISGLASRIIFEDIVMY VKNPIIIDQTYGTKK
Sbjct: 362 KGVYDVLVKNCTIFNATNGARIKTWASPISGLASRIIFEDIVMYGVKNPIIIDQTYGTKK 421

Query: 374 KKVNN 379
            + +N
Sbjct: 422 SEESN 426

BLAST of CSPI07G14680 vs. NCBI nr
Match: KAA0048661.1 (exopolygalacturonase-like [Cucumis melo var. makuwa] >KAA0048665.1 exopolygalacturonase-like [Cucumis melo var. makuwa] >TYK18163.1 exopolygalacturonase-like [Cucumis melo var. makuwa])

HSP 1 Score: 674.5 bits (1739), Expect = 5.5e-190
Identity = 338/382 (88.48%), Postives = 351/382 (91.88%), Query Frame = 0

Query: 1   MTRNLSL-IQILLLVFAWQCCAVV---FDHSAGTQNRLKGIVPTTNEQFLRPAEAPRRLG 60
           MTRNLSL IQILLLVFA QC A V    D   GT+N LK  VPT NEQFLRPA APRRLG
Sbjct: 1   MTRNLSLVIQILLLVFASQCSAKVAATLDDVTGTENLLKVTVPTVNEQFLRPAGAPRRLG 60

Query: 61  FSTLSNIGSTVNDIKANVDLNENGVSVFDVTKHGAKANGKTDDAQAFMTTWIAACRNTVG 120
             TL +IGSTVNDI+AN+ LNENG SVFDVTKHGAKA+G+TDDAQAFMTTWIAACRNTVG
Sbjct: 61  IGTLPDIGSTVNDIRANIVLNENGGSVFDVTKHGAKADGETDDAQAFMTTWIAACRNTVG 120

Query: 121 PAKFLIPQGTFLVGPVTFAGPCKSFPITLENQGTVKATTDISAYSSPEWFSIEDITGFIL 180
           PAKFLIPQGT+LVGPVTFAGPCKSFPITLENQGTVKATTDIS YSSPEWFSIEDITGFIL
Sbjct: 121 PAKFLIPQGTYLVGPVTFAGPCKSFPITLENQGTVKATTDISEYSSPEWFSIEDITGFIL 180

Query: 181 TGSGVFDGQGLSVWSYNDCKKNNLCQLLPISIKFMRLNHTIVDGLTSINSMGFHTSVFYC 240
           TGSGVFDGQG++VW YNDCKKN  CQLLPISIKF RLNHTIVDGLTS+NSMGFHTSVFYC
Sbjct: 181 TGSGVFDGQGVTVWPYNDCKKNRFCQLLPISIKFSRLNHTIVDGLTSVNSMGFHTSVFYC 240

Query: 241 YNFTATNMKIIAPHNSPNTDGMHLSTSKLVTIANSVIGTGDDCVSIGHSTESITVTNVTC 300
           YNFTATNMKIIAPHNSPNTDGMHLSTSKLVTI NSVIGTGDDCVSIGHSTE+I VTNVTC
Sbjct: 241 YNFTATNMKIIAPHNSPNTDGMHLSTSKLVTITNSVIGTGDDCVSIGHSTENIVVTNVTC 300

Query: 301 GPGHGLSVGSLGKYSKEKGVYDVLVKNCTIFNATNGARIKTWASPISGLASRIIFEDIVM 360
           GPGHGLSVGSLGKYSKEK VY+VLVKNCTIFNATNGARIKTWAS ISGLAS IIFEDIVM
Sbjct: 301 GPGHGLSVGSLGKYSKEKSVYNVLVKNCTIFNATNGARIKTWASRISGLASGIIFEDIVM 360

Query: 361 YNVKNPIIIDQTYGTKKKKVNN 379
           YNVKNPIIIDQTYGTKKKKV+N
Sbjct: 361 YNVKNPIIIDQTYGTKKKKVSN 382

BLAST of CSPI07G14680 vs. TAIR 10
Match: AT1G02790.1 (polygalacturonase 4 )

HSP 1 Score: 291.2 bits (744), Expect = 1.2e-78
Identity = 143/296 (48.31%), Postives = 192/296 (64.86%), Query Frame = 0

Query: 77  NENGVSVFDVTKHGAKANGKTDDAQAFMTTWIAACRNTVGPAKFLIPQGTFLVGPVTFAG 136
           N    +V+D+TK GA  +G T+  +AF+ TWI  C + V PA  L+P+GTFL GPV FAG
Sbjct: 45  NSGNPTVYDITKFGAVGDGSTNTFKAFLNTWIQVCDSPV-PATLLVPKGTFLAGPVIFAG 104

Query: 137 PCKSFPITLENQGTVKATTDISAYSSPEWFSIEDITGFILTGSGVFDGQGLSVWSYNDCK 196
           PCKS  +T+   GT+ ATT  S Y++PEWF  E +   +LTG+G F G+G +VW  + C 
Sbjct: 105 PCKS-KVTVNVIGTIIATT--SGYATPEWFLFERVDNLVLTGTGTFHGKGEAVWKADGCG 164

Query: 197 KNNLCQLLPISIKFMRLNHTIVDGLTSINSMGFHTSVFYCYNFTATNMKIIAPHNSPNTD 256
           K   C L P S+KF  + +  ++G++S+N+  FH  +    N    N+K+ AP  SPNTD
Sbjct: 165 KKVQCNLPPTSLKFRNMKNVEINGISSVNAKAFHMFLVKTENVNIQNIKLTAPAESPNTD 224

Query: 257 GMHLSTSKLVTIANSVIGTGDDCVSIGHSTESITVTNVTCGPGHGLSVGSLGKYSKEKGV 316
           G+HLS +  V+I +S I TGDDCVS+G  + ++TV  V CGPGHGLSVGSLGKY  E+ V
Sbjct: 225 GIHLSNADNVSILDSTIATGDDCVSVGRGSNNVTVERVICGPGHGLSVGSLGKYKNEEDV 284

Query: 317 YDVLVKNCTIFNATNGARIKTWASPISGLASRIIFEDIVMYNVKNPIIIDQTYGTK 373
             + V NCT+    NG RIKTW       A  I FE+I+M +VKNPIIIDQ YG++
Sbjct: 285 SGIHVNNCTMIETDNGLRIKTWGGSDPSKAVDIKFENIIMQSVKNPIIIDQNYGSR 336

BLAST of CSPI07G14680 vs. TAIR 10
Match: AT4G18180.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 273.1 bits (697), Expect = 3.4e-73
Identity = 137/297 (46.13%), Postives = 190/297 (63.97%), Query Frame = 0

Query: 76  LNENGVSVFDVTKHGAKANGKTDDAQAFMTTWIAACRNTVGPAKFLIPQGTFLVGPVTFA 135
           ++++ V + DV   GA+AN   D  +AF+  W  AC+++      +IP+G F VG + F+
Sbjct: 27  VDKSSVFLVDVRSFGARANDHRDHTKAFVAAWDKACKSSSSSVNLIIPRGEFSVGSLRFS 86

Query: 136 GPCKSFP-ITLENQGTVKATTDISAY-SSPEWFSIEDITGFILTGSGVFDGQGLSVWSYN 195
           GPC +   +T+     VKA+TD+S Y S   W     I G  LTG G FDGQG   W +N
Sbjct: 87  GPCTNVSNLTVR----VKASTDLSKYRSGGGWIQFGWINGLTLTGGGTFDGQGALAWPFN 146

Query: 196 DCKKNNLCQLLPISIKFMRLNHTIVDGLTSINSMGFHTSVFYCYNFTATNMKIIAPHNSP 255
           +C  ++ C+LLP S+KF+ +N T+V  ++S+NS  FH ++  C +F  T + I AP +SP
Sbjct: 147 NCTSDSNCKLLPTSLKFVGMNRTVVRRISSVNSKFFHIALVECRDFKGTRLNITAPSDSP 206

Query: 256 NTDGMHLSTSKLVTIANSVIGTGDDCVSIGHSTESITVTNVTCGPGHGLSVGSLGKYSKE 315
           NTDG+H+  S  V  + S I TGDDCVSIG     IT+T++ CGPGHG+SVGSLG+Y  E
Sbjct: 207 NTDGIHIERSSNVYFSRSHIATGDDCVSIGQGNSQITITSIKCGPGHGISVGSLGRYPNE 266

Query: 316 KGVYDVLVKNCTIFNATNGARIKTWA-SPISGLASRIIFEDIVMYNVKNPIIIDQTY 370
           K V  ++VK+C I   TNG RIKTWA SP    A+ + FE+I+M NV NPIIIDQ+Y
Sbjct: 267 KDVNGLVVKDCKISGTTNGIRIKTWANSPGLSAATNMTFENIIMNNVTNPIIIDQSY 319

BLAST of CSPI07G14680 vs. TAIR 10
Match: AT3G14040.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 257.7 bits (657), Expect = 1.5e-68
Identity = 136/310 (43.87%), Postives = 180/310 (58.06%), Query Frame = 0

Query: 63  IGSTVNDIKANVDLNENGVSVFDVTKHGAKANGKTDDAQAFMTTWIAACRNTVGPAKFLI 122
           +G     + A V   + G +  DV   GAK +GKTDD+ AF   W  AC      +   +
Sbjct: 50  VGGAAASVGAKVTGAKPGGASLDVKASGAKGDGKTDDSAAFAAAWKEAC---AAGSTITV 109

Query: 123 PQGTFLVGPVTFAGPCKSFPITLENQGTVKATTDISAYSSPE--WFSIEDITGFILTGS- 182
           P+G +LV  + F GPCK  P+TLE  G  KA   +   + P   W   E++  F L G+ 
Sbjct: 110 PKGEYLVESLEFKGPCKG-PVTLELNGNFKAPATVKT-TKPHAGWIDFENLADFTLNGNK 169

Query: 183 GVFDGQGLSVWSYNDCKKNNLCQLLPISIKFMRLNHTIVDGLTSINSMGFHTSVFYCYNF 242
            +FDGQG   W  NDC K   C  LPI+I+F  L ++ ++ +TS NS  FH ++  C N 
Sbjct: 170 AIFDGQGSLAWKANDCAKTGKCNSLPINIRFTGLTNSKINSITSTNSKLFHMNILNCKNI 229

Query: 243 TATNMKIIAPHNSPNTDGMHLSTSKLVTIANSVIGTGDDCVSIGHSTESITVTNVTCGPG 302
           T T++ I AP  S NTDG+H+  S  V +  + I TGDDCVSIG  TE++ V NV CGPG
Sbjct: 230 TLTDIGIDAPPESLNTDGIHIGRSNGVNLIGAKIKTGDDCVSIGDGTENLIVENVECGPG 289

Query: 303 HGLSVGSLGKYSKEKGVYDVLVKNCTIFNATNGARIKTWASPISGLASRIIFEDIVMYNV 362
           HG+S+GSLG+Y  E+ V  V V+ C I N  NG RIKTW     G+AS I+FEDI M NV
Sbjct: 290 HGISIGSLGRYPNEQPVKGVTVRKCLIKNTDNGVRIKTWPGSPPGIASNILFEDITMDNV 349

Query: 363 KNPIIIDQTY 370
             P++IDQ Y
Sbjct: 350 SLPVLIDQEY 354

BLAST of CSPI07G14680 vs. TAIR 10
Match: AT3G07850.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 253.1 bits (645), Expect = 3.7e-67
Identity = 136/317 (42.90%), Postives = 181/317 (57.10%), Query Frame = 0

Query: 59  TLSNIGSTVNDIKANVDLNENGV---SVFDVTKHGAKANGKTDDAQAFMTTWIAACRNTV 118
           T  N  + V    A+V    +G    +  DV   GAK + KTDD+ AF   W  AC    
Sbjct: 42  TAKNAATAVGGAAASVGAKVSGAKPGAAVDVKASGAKGDSKTDDSAAFAAAWKEAC---A 101

Query: 119 GPAKFLIPQGTFLVGPVTFAGPCKSFPITLENQGTVKATTDISAYSSPE--WFSIEDITG 178
             +   +P+G ++V  + F GPCK  P+TLE  G  KA   +   + P   W   E+I  
Sbjct: 102 AGSTITVPKGEYMVESLEFKGPCKG-PVTLELNGNFKAPATVKT-TKPHAGWIDFENIAD 161

Query: 179 FILTGS-GVFDGQGLSVWSYNDCKKNNLCQLLPISIKFMRLNHTIVDGLTSINSMGFHTS 238
           F L G+  +FDGQG   W  NDC K   C  LPI+I+F  L ++ ++ +TS NS  FH +
Sbjct: 162 FTLNGNKAIFDGQGSLAWKANDCAKTGKCNSLPINIRFTGLTNSKINSITSTNSKLFHMN 221

Query: 239 VFYCYNFTATNMKIIAPHNSPNTDGMHLSTSKLVTIANSVIGTGDDCVSIGHSTESITVT 298
           +  C N T +++ I AP  S NTDG+H+  S  V +  + I TGDDCVSIG  TE++ V 
Sbjct: 222 ILNCKNITLSDIGIDAPPESLNTDGIHIGRSNGVNLIGAKIKTGDDCVSIGDGTENLIVE 281

Query: 299 NVTCGPGHGLSVGSLGKYSKEKGVYDVLVKNCTIFNATNGARIKTWASPISGLASRIIFE 358
           NV CGPGHG+S+GSLG+Y  E+ V  V V+ C I N  NG RIKTW     G+AS I+FE
Sbjct: 282 NVECGPGHGISIGSLGRYPNEQPVKGVTVRKCLIKNTDNGVRIKTWPGSPPGIASNILFE 341

Query: 359 DIVMYNVKNPIIIDQTY 370
           DI M NV  P++IDQ Y
Sbjct: 342 DITMDNVSLPVLIDQEY 353

BLAST of CSPI07G14680 vs. TAIR 10
Match: AT2G26620.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 243.8 bits (621), Expect = 2.2e-64
Identity = 125/289 (43.25%), Postives = 177/289 (61.25%), Query Frame = 0

Query: 83  VFDVTKHGAKANGKTDDAQAFMTTWIAACRNTVGPAKFLIPQGTFLVGPVTFAGPCKSFP 142
           VF+V +HG+K +GKTD+  AF + W  AC+   G +K  +P+GTF +G V F GPCK+ P
Sbjct: 30  VFNVQRHGSKPDGKTDNTNAFTSIWSRACKRISGSSKIYVPKGTFYLGGVEFVGPCKN-P 89

Query: 143 ITLENQGTVKATTDISAYSSPEWFSIEDITGFILTGSGVFDGQGLSVWSYNDCKKNNLCQ 202
           I     GT+ A  + +      W +   I    ++GSG  DGQG   W  NDC KN  C 
Sbjct: 90  IEFIIDGTLLAPANPNDIKQDTWINFRYINNLSISGSGTLDGQGKQSWPLNDCHKNPNCP 149

Query: 203 LLPISIKFMRLNHTIVDGLTSINSMGFHTSVFYCYNFTATNMKIIAPHNSPNTDGMHLST 262
            L IS+ F  +N++ +  +TS+NS   H + F+ ++F  T + I AP +SPNTDG+ + +
Sbjct: 150 KLAISMGFAFVNNSNIKDITSLNSKMGHFNFFFVHHFNITGVTITAPSDSPNTDGIKMGS 209

Query: 263 SKLVTIANSVIGTGDDCVSIGHSTESITVTNVTCGPGHGLSVGSLGKYSKEKGVYDVLVK 322
              + I+N+ IGTGDDC++I   T  + ++N+ CGPGHG+SVGSLGK   EK V D+ V+
Sbjct: 210 CSNIQISNTNIGTGDDCIAILSGTTKLNISNINCGPGHGISVGSLGKNKDEKDVKDLFVR 269

Query: 323 NCTIFNAT-NGARIKTWASPISG-LASRIIFEDIVMYNVKNPIIIDQTY 370
           +  IFN T +G RIKTW S  S  L S  ++E+I M +V  PI IDQ Y
Sbjct: 270 D-VIFNGTSDGIRIKTWESSASKILVSNFVYENIQMIDVGKPINIDQKY 316

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P490621.7e-7748.31Exopolygalacturonase clone GBGE184 OS=Arabidopsis thaliana OX=3702 GN=PGA3 PE=2 ... [more]
Q397861.2e-7046.13Polygalacturonase OS=Gossypium hirsutum OX=3635 GN=G9 PE=2 SV=1[more]
Q397662.6e-7045.77Polygalacturonase OS=Gossypium barbadense OX=3634 GN=G9 PE=2 SV=1[more]
P353391.0e-6946.23Exopolygalacturonase OS=Zea mays OX=4577 GN=PG2C PE=2 SV=1[more]
Q6H9K01.7e-6944.79Exopolygalacturonase (Fragment) OS=Platanus acerifolia OX=140101 GN=plaa2 PE=1 S... [more]
Match NameE-valueIdentityDescription
A0A5A7U4W12.7e-19088.48Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... [more]
A0A5A7TQ894.5e-19088.22Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffo... [more]
A0A0A0KMA61.0e-18693.64Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G222970 PE=3 SV=1[more]
A0A0A0K5I25.4e-16783.24Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G398220 PE=3 SV=1[more]
A0A1S3BRD32.3e-16277.39exopolygalacturonase-like OS=Cucumis melo OX=3656 GN=LOC103492867 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_031745094.15.6e-21196.30exopolygalacturonase-like [Cucumis sativus][more]
XP_031745095.11.6e-21096.80exopolygalacturonase-like [Cucumis sativus][more]
XP_031741281.14.8e-20292.86exopolygalacturonase [Cucumis sativus][more]
XP_031745103.15.7e-19591.78exopolygalacturonase-like [Cucumis sativus][more]
KAA0048661.15.5e-19088.48exopolygalacturonase-like [Cucumis melo var. makuwa] >KAA0048665.1 exopolygalact... [more]
Match NameE-valueIdentityDescription
AT1G02790.11.2e-7848.31polygalacturonase 4 [more]
AT4G18180.13.4e-7346.13Pectin lyase-like superfamily protein [more]
AT3G14040.11.5e-6843.87Pectin lyase-like superfamily protein [more]
AT3G07850.13.7e-6742.90Pectin lyase-like superfamily protein [more]
AT2G26620.12.2e-6443.25Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006626Parallel beta-helix repeatSMARTSM00710pbh1coord: 236..262
e-value: 8500.0
score: 0.6
coord: 263..284
e-value: 1100.0
score: 6.8
coord: 316..337
e-value: 10.0
score: 15.1
coord: 346..367
e-value: 2500.0
score: 4.4
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 73..382
e-value: 8.6E-88
score: 297.0
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 115..375
e-value: 3.7E-76
score: 256.3
IPR000743Glycoside hydrolase, family 28PROSITEPS00502POLYGALACTURONASEcoord: 293..306
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 1..374
NoneNo IPR availablePANTHERPTHR31375:SF158POLYGALACTURONASEcoord: 1..374
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 83..373

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI07G14680.1CSPI07G14680.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0004650 polygalacturonase activity