CSPI07G14180 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI07G14180
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	E3 ubiquitin-protein ligase PRT1
Location	Chr7: 12691072 .. 12697058 (+)
RNA-Seq Expression	CSPI07G14180
Synteny	CSPI07G14180

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GACTTCGACAAACCTATTCATCCAAAATATCTCTAAGCCAAGCAAATCCAACCCATTTAGAGTTACTATTATGATTTTGATTTTGGTTGGTTACGGACTTGGCGTTCCGAGTAATTACAGGCTGCCATTTGACTCTAATCTGCTCTGTTAATCAGAATCCCCCAGTTTCACGTTTCCCTCTATTCACCGCCATTTCTTTTCTCTCCTCCGATTCCCTTCTCAACATTCTCCTCAACCTTATCAATGGCGGAATATCAGATGCTTGACGATGTCGACGCCGACGCATTCCTCTGCTGTGTCTGCTTGTCAGCTCCCTCTCTCCCTCTCTCTTGTTTTACCTTCATTTTTGGAATCAAAATACGCCTTGGAATGATTTTTTGTTCGATGTTTGTATATATTTATGTGGGTTTCTTGTTTTGTTCTGATGGTTTATTGTTGAGGTTAGGAATTTGAACAATCAATTATTTTCAATGGCAAAGCTAATGATTTCTTGTTCTGGGTCATCTTCGATTTGCGATGTGCTTATATGTTAATAGCTATGCCGTGTATTTTACGTGTTCGGTGTATACTTTCTTAAGAAGAGGCGAGGCTTTCAGGAAGGCTATCATTATTAAACGTTGTTTCTCAAAATCCCATTTTGGGATGCCTAGTATTTTTCTATTTGCGTGAATTGCCCTATTCCTCGTTTGGCCCGAAAGTCACCTAGGTGGGTTGGTAAATAATAGAATTGGATAAAAAGGACTCGTTTCATTTTGTCAAAGCATTGTTGTCATCTATTGGATCCAAAGTGAACACTCGATGTAAACTTTTGAGTTGGGGTTGACTGGTTTTTAGTTATTCTTTCAAGAACCAGATGTGGCGTCCCACTCTGACTGATTCGGAAGCTCGAACCTACCCTAAGATTTAGACACTGAGAGACATCAAGGACTTTTTTGTTTTCTTATTAAGCTCACTCAATTGTTTGCATGCTCATTTGAAGTCATTGTAAAGTTTAGAAACAGTTGGCTTCATGTTAACTATTCTTTGGCTGAGGGATACATAGTAGAGTGGGACATTGCATTTGGAATTTGTTCATGTTCAATACTATGATAAATTGTAACCATCATAGGTTAGCTTAGTGGTAAAAAGGGAGACATAGTTTCAATAACTAACTTAGAGGTTATAGCTTCAATCCATGATGGTCATCTACCCAGAATTAATTTCTATGAGCTTTCTTGACATCCAAATGTTGTAGTGTCAGACGGTTATCCCACGCGAGATTAGTTGAGGTGCGTAAGCTGGCCCGAACACTCACATATATATAAGAAAATATACTAGCATAAATTGTAGGAATGACTTCAAATAACTAAAGAATGGATGGTCTATATCAATTCAGCTCTTTTCAAGATCTCAGTAATGACTTCAAACAAGCATTGACTAGTTTTCTTTTGATAAGATTTCTGCACGTATTGCCAAATAATGGAGGCCCCTTTCTATTGCATACTTTTTGTTCCTGAATTATGCCAAATAAATGTGTACATAGTAGGCAATTTCAATTTGTATAATTTATCTTCTAAGAGTCCCTTATCCAATAGGACTACAGTTCTAAACTGGGTTTTCCTAGATTTTGGAGGAAAGTTAAACTTTGAATTGACCATTACATGATCATGAGGTATTGGCTTATAGTATTATTAGAAACGAGAAACAACTGGGTGCAAGGAGGTTGTTTTATTCTGTTGTTTGTTGGTATTATTTGTCTTCACCAGTTTCTTCTTCATATGGCTAATTTAAGTTGGTTGTGAATTGCAGGGATCTTCTCTACAAGCCTATAGTGCTCCGTGAGTATAATTAGATGTTGACCATACATTTTTTTGAGAGGTGTCATCCTTTTGTTAATTTTTAGTTTCTTCTATTCCAGCTTGTGGTCATATTTCATGTTTTTGGTGCGTGCACAAATGCATGAATGGCTTTCATGAGTCCCACTGTCCAATTTGTAGACGTTCATATTATCACTTTCCAACCATCTGTGAGATTCTTCACCAATTGATCCTGAAGATTTATCCCGCATCCTATAAGAGAAGGGAAAGTCAAATTCTAGGTGAGCTTTCTAGTGGATGCTGTTATCACTAATGTGCTAATGTCTGCAACGGCTGCAATCATGGCTGTTAAAAACAATATTATATGACTGAGGTGTAATGGTTTTAGCTTTGAATCTGTTAAGTGTCTACTTCACCTGCAGTCAGTAAAACCCAACGAAAAGTAGAGCAAAGAACAGGGAAAGTCGATCCCTGTTATGATGATGTATTTAAATGCCAATGAGATCAAAGTTGCAAAGCCAGAATAACACAAAGTACTGGCTTGAAAATGGGCTTATCTATTTAATGTACAAGGGAACACATCTTTTTGAGCCGCTTATCTATCTAGTGTACCTGTTTTTTATATTTTCTTGACAAGACTGCTAGTGCCCTTGCTGTATTCCTTGATTAATTTAATAATTTGTAAGATTTATCTTGTCTTCCATCTTTCAGATTATTTATTTTTTGCTTAATTCTTTTTATCAATGATAATAATGTTTCATAATGAAAAAACTAATTAGAATGCAGAATGCTAATGATCCATTACTTTTTGCGTTTGATATTAAATATTAATTGTGTTAAAATACACATAAATCTCAAACATATTGTTTAATTGATGGAACTGTATTCATGAAAATGATATTTTCTTAATGTCGGTTCAACAAATGAGTAGTCATTCATTGTCTATTTTCTTCAAATTTTTTGGTAGAGTTATTTTAACCTTGTCACCATTTAAATGATTTTCATTTTCAGAGGTGGAAAAGAAAATTGGATTTTTCTCTCCACAATTTGATTCCCTTGCTTGTGGCTCCCAAGCTGGTATGAAAGTGGAACACTTAGAAGATTCTGCTAATGGCGAGCTGAACACAAATACCAAAAATGATGACGCTGTTGCTGAATTAATATTAGAAGAAAATTCTGACGTTGTTAGTTCTACTTCTGTTGTAAGCCTCAATTCTCTTCAAGATCCTTGTGCTCAAAAGACTCAAAATCAGGAGAAAATTTCAGTTGCTGATGTGCTATGTCAAGCTTGCACCCAATTGCTCTTCCGACCTGTGGTTATGAACTGTGGCCATGGTATATATTCTTTTTTTTCATCCCTGTGTGAGCTCGCATTCTATTTTACCTTCAGTTATGTAGTAAAAGTATTTGTTTAAGTATCGGGTTATTTCATTTTTCAACTTTACTACCATTCCCAAAATATGTTGACTTCAATGCCATAAATTAAAAGGTTTCAATATAACTTATTTTCTTATGTTTATAGTATTTTGTGAGAGTTGTATCAACTCACAAGTTGAGACACTTGAGTGCCAAGTTTGTCAGTCGCTGCAACCAAGGGGATTTCGAAATGTGTGTTTGGAGCTTGATCAGTTTTTGAAGGAAAAATTTCCTGAAGAATATAGCATCCGCAGAGACTCAGTTCAACTTAAACTAGCCAACAGTATGAAGAATGACAATCCAACTTCCTGTATGTTTGTTTACACTTGCTTAAATAATCGTTTTTAATATTGTTGCATTTGTGTTATTGGATTAATCTATTTTGTTGCTTTTAGTTCTTGGTATTTGTTACATTCTTCTTCTTTTTTTCTTAATTTCTAGTTCACGTATTTATTGCACTTTTGTTTGTGCGGACTGAAGGTAGTTTAGCTGTCCAAACTATGTAATTGTTAGAGGTTGTAGGAAAATTTCTTTGTCAATGATTTTGAATGATCGTATCCATTTATTTCTGGTATTTCTACATCATCTTTGAAGTGATGGAATGATTCATTCAAAATGGAAAATGTACTTATAATGCTTTTTAAAACATTATTTCTGGTATTTCTACATCATCTCCGAAAACCCGCATCATATTATCAAAATTTACTAATGTTTTTAGGTCCATTTATCTGATAGCAGGGCTGTTCTACTACCATCCATGCTCTATTCATTTCTTTCATATTGGTAATTTCAGGAAAACAAGATATTGTACACATGTAGGAATTTTGAAATAATTGTGTTATTTACAATTGTATTCTTCAATCCTTTTGGTAAGGGTAGAAATGTACTTATAATACTTGATGGCGTACTGTTGCTCTACAATATTGTAAATGCTTCCTTAACACAAACTAGTCTGTATAATATCATTTTGGTTTATTTACGTTTATTATTTGCTTCCAGGTTCCAATGAAGAGGGTAAAAAGGGACAGTACCTGCCAAGATGGGATGATGTTGCCTCTAAAGTGCATACTTTTATTGGCTGTGATTATTGTGGGGTAATTTCCTTATATGTTCACCATTCTTATCATGCTGTCAATGTTGTTAAAAGGCTATTTAGTGTAGCTTAGTGATAAAAGGAGATCAATAAAACCAAAGTCTTTATTATGGATCAAAATTTATCAATACCAAAAGATAATCCATTTATTTATGGAGAATAGGAAAACAAGGTAATCCTAATCCAAATTTATGAAAAAAAGTAATCCTAAACCAAACTAATGAAGGAAACTAATCCGTATCCTAATCAATCAAGGGAACTTATCCTAATTCTAATTAATCAAGGATTGTAATCTGATAGACCTCCTATCGTTCACCAATACAGTAGAAGAGGAAAGAAGGCTAAGGATGGCAGGTGCAGATGAAGGAGTCTGTTAGTAAAGAGGTCTGTTAGTAGTCTGTTAAGTAAAGAGGCGGTTAGAAGTATGGGTATAAATAGATTGTGTTTTAGGAGGGAAAGTTAGTTAAGATCTTGGTAGAGAGAGAAGTCATCTGTGCACCTTTGGAAGACAAGGTAACAGAGAGAGAGTGAGGAAGACTGTAATCGAATTGAGAAATTCTCAATTTGATCTTTTCATACTATTGATTCTTGTATTCTGTTCAGACACTATTACATCAATAAAGTTCTATTTATTGAACTCAAGTTCGGGTCCCAACATAATCACTCCCAAACATGGCCTTACCTAATAAAATAGTTGCATTTCATACACCATGGACTTGGTGAATAGATATATGGAGATTTAACATAATGTCTCATATGAGGATAACCTATAGCTCCTCTGCCTGAACTAAAAATTGGATCCAAATATTGAAACTAGTTTGCTGACTAGAATTTGATTACCAGGTGCTCACTTTGTTACTGATCGTAATATTCTTTTGTTATACACTGCAGATGTTTCCCCTCATTGGCGACAGATACAAGTGCAAGGATTGTTTAGAGGCATCAGGTTTTGATCTCTGTGGTGACTGCTACAATACACGTTCAAAACGTCCCGGTCGGTTTAACCAGCAACATAGACCAGAGCACAGGTTCCAGCTTGTACATCCTTCAATGTTTCAAAATATGACGCAGGGGTAGTGGCAGAAATGTTAGAGGAAGGTTCTACAACCTTTATTATTGCTAATGGTGAAGGTTCAGATAGCTCAGATAACATAGGTGCAATTCTCGAATTTTCGAGCGATGCTCGTGAGAGTGATGAGGATGATTCCGACGTGGATGGTAGAACTCAAACAAACTCCAATGACAATAGAACTGATCAAAATCACTCTGAACCCACCTAAAATGTTAAAATATCTGGAATCAAAATACAACATTGCTTCGTATGGAGTTCCCACTCTGGTTTTCGTAACACGATCTCTTTCTCTAGTGTCTGGATGATTCATAAACTGAAATTTCTATGTTTTTTTACGAGGGAAGATGATAAAAGAGGCATAAAAGACCTCACATCAATGTACATAAAAGTGTCCTCTAATCCTCTTCAAAATTCAGCTGCTTCCTCTTCCTCTTGAATTTGAACAAACAGTGGAGTCATCTGAGGTTGGTTTTTTCTTATAATTAGCAACAGCCAACTTGTAAGTATATTAATGGTGGTGGTTTATTCAAATAACATTTTCTGAGCAAACTTGGAAAATGAGCTTTCATTTTAGTTTTTAGTGTTATTGCACAAAGAGTTATTATCAGGT

mRNA sequence

GACTTCGACAAACCTATTCATCCAAAATATCTCTAAGCCAAGCAAATCCAACCCATTTAGAGTTACTATTATGATTTTGATTTTGGTTGGTTACGGACTTGGCGTTCCGAGTAATTACAGGCTGCCATTTGACTCTAATCTGCTCTGTTAATCAGAATCCCCCAGTTTCACGTTTCCCTCTATTCACCGCCATTTCTTTTCTCTCCTCCGATTCCCTTCTCAACATTCTCCTCAACCTTATCAATGGCGGAATATCAGATGCTTGACGATGTCGACGCCGACGCATTCCTCTGCTGTGTCTGCTTGGATCTTCTCTACAAGCCTATAGTGCTCCCTTGTGGTCATATTTCATGTTTTTGGTGCGTGCACAAATGCATGAATGGCTTTCATGAGTCCCACTGTCCAATTTGTAGACGTTCATATTATCACTTTCCAACCATCTGTGAGATTCTTCACCAATTGATCCTGAAGATTTATCCCGCATCCTATAAGAGAAGGGAAAGTCAAATTCTAGAGGTGGAAAAGAAAATTGGATTTTTCTCTCCACAATTTGATTCCCTTGCTTGTGGCTCCCAAGCTGGTATGAAAGTGGAACACTTAGAAGATTCTGCTAATGGCGAGCTGAACACAAATACCAAAAATGATGACGCTGTTGCTGAATTAATATTAGAAGAAAATTCTGACGTTGTTAGTTCTACTTCTGTTGTAAGCCTCAATTCTCTTCAAGATCCTTGTGCTCAAAAGACTCAAAATCAGGAGAAAATTTCAGTTGCTGATGTGCTATGTCAAGCTTGCACCCAATTGCTCTTCCGACCTGTGGTTATGAACTGTGGCCATGTATTTTGTGAGAGTTGTATCAACTCACAAGTTGAGACACTTGAGTGCCAAGTTTGTCAGTCGCTGCAACCAAGGGGATTTCGAAATGTGTGTTTGGAGCTTGATCAGTTTTTGAAGGAAAAATTTCCTGAAGAATATAGCATCCGCAGAGACTCAGTTCAACTTAAACTAGCCAACAGTATGAAGAATGACAATCCAACTTCCTGTTCCAATGAAGAGGGTAAAAAGGGACAGTACCTGCCAAGATGGGATGATGTTGCCTCTAAAGTGCATACTTTTATTGGCTGTGATTATTGTGGGATGTTTCCCCTCATTGGCGACAGATACAAGTGCAAGGATTGTTTAGAGGCATCAGGTTTTGATCTCTGTGGTGACTGCTACAATACACGTTCAAAACGTCCCGGTCGGTTTAACCAGCAACATAGACCAGAGCACAGGTTCCAGCTTGTACATCCTTCAATGTTTCAAAATATGACGCAGGGGTAGTGGCAGAAATGTTAGAGGAAGGTTCTACAACCTTTATTATTGCTAATGGTGAAGGTTCAGATAGCTCAGATAACATAGGTGCAATTCTCGAATTTTCGAGCGATGCTCGTGAGAGTGATGAGGATGATTCCGACGTGGATGGTAGAACTCAAACAAACTCCAATGACAATAGAACTGATCAAAATCACTCTGAACCCACCTAAAATGTTAAAATATCTGGAATCAAAATACAACATTGCTTCGTATGGAGTTCCCACTCTGGTTTTCGTAACACGATCTCTTTCTCTAGTGTCTGGATGATTCATAAACTGAAATTTCTATGTTTTTTTACGAGGGAAGATGATAAAAGAGGCATAAAAGACCTCACATCAATGTACATAAAAGTGTCCTCTAATCCTCTTCAAAATTCAGCTGCTTCCTCTTCCTCTTGAATTTGAACAAACAGTGGAGTCATCTGAGGTTGGTTTTTTCTTATAATTAGCAACAGCCAACTTGTAAGTATATTAATGGTGGTGGTTTATTCAAATAACATTTTCTGAGCAAACTTGGAAAATGAGCTTTCATTTTAGTTTTTAGTGTTATTGCACAAAGAGTTATTATCAGGT

Coding sequence (CDS)

ATGGCGGAATATCAGATGCTTGACGATGTCGACGCCGACGCATTCCTCTGCTGTGTCTGCTTGGATCTTCTCTACAAGCCTATAGTGCTCCCTTGTGGTCATATTTCATGTTTTTGGTGCGTGCACAAATGCATGAATGGCTTTCATGAGTCCCACTGTCCAATTTGTAGACGTTCATATTATCACTTTCCAACCATCTGTGAGATTCTTCACCAATTGATCCTGAAGATTTATCCCGCATCCTATAAGAGAAGGGAAAGTCAAATTCTAGAGGTGGAAAAGAAAATTGGATTTTTCTCTCCACAATTTGATTCCCTTGCTTGTGGCTCCCAAGCTGGTATGAAAGTGGAACACTTAGAAGATTCTGCTAATGGCGAGCTGAACACAAATACCAAAAATGATGACGCTGTTGCTGAATTAATATTAGAAGAAAATTCTGACGTTGTTAGTTCTACTTCTGTTGTAAGCCTCAATTCTCTTCAAGATCCTTGTGCTCAAAAGACTCAAAATCAGGAGAAAATTTCAGTTGCTGATGTGCTATGTCAAGCTTGCACCCAATTGCTCTTCCGACCTGTGGTTATGAACTGTGGCCATGTATTTTGTGAGAGTTGTATCAACTCACAAGTTGAGACACTTGAGTGCCAAGTTTGTCAGTCGCTGCAACCAAGGGGATTTCGAAATGTGTGTTTGGAGCTTGATCAGTTTTTGAAGGAAAAATTTCCTGAAGAATATAGCATCCGCAGAGACTCAGTTCAACTTAAACTAGCCAACAGTATGAAGAATGACAATCCAACTTCCTGTTCCAATGAAGAGGGTAAAAAGGGACAGTACCTGCCAAGATGGGATGATGTTGCCTCTAAAGTGCATACTTTTATTGGCTGTGATTATTGTGGGATGTTTCCCCTCATTGGCGACAGATACAAGTGCAAGGATTGTTTAGAGGCATCAGGTTTTGATCTCTGTGGTGACTGCTACAATACACGTTCAAAACGTCCCGGTCGGTTTAACCAGCAACATAGACCAGAGCACAGGTTCCAGCTTGTACATCCTTCAATGTTTCAAAATATGACGCAGGGGTAG

Protein sequence

MAEYQMLDDVDADAFLCCVCLDLLYKPIVLPCGHISCFWCVHKCMNGFHESHCPICRRSYYHFPTICEILHQLILKIYPASYKRRESQILEVEKKIGFFSPQFDSLACGSQAGMKVEHLEDSANGELNTNTKNDDAVAELILEENSDVVSSTSVVSLNSLQDPCAQKTQNQEKISVADVLCQACTQLLFRPVVMNCGHVFCESCINSQVETLECQVCQSLQPRGFRNVCLELDQFLKEKFPEEYSIRRDSVQLKLANSMKNDNPTSCSNEEGKKGQYLPRWDDVASKVHTFIGCDYCGMFPLIGDRYKCKDCLEASGFDLCGDCYNTRSKRPGRFNQQHRPEHRFQLVHPSMFQNMTQG*

Homology

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BLAST of CSPI07G14180 vs. ExPASy Swiss-Prot
Match: Q8LBL5 (E3 ubiquitin-protein ligase PRT1 OS=Arabidopsis thaliana OX=3702 GN=PRT1 PE=2 SV=2)

HSP 1 Score: 318.9 bits (816), Expect = 7.2e-86
Identity = 164/353 (46.46%), Postives = 222/353 (62.89%), Query Frame = 0


Query: 13  DAFLCCVCLDLLYKPIVLPCGHISCFWCVHKCMNGFHESHCPICRRSYYHFPTICEILHQ 72
           D FLCCVCL+LLYKPIVL CGH+SCFWCVHK MNGF ESHCPICR  Y HFP++C+ L+ 
Sbjct: 22  DQFLCCVCLELLYKPIVLSCGHLSCFWCVHKSMNGFRESHCPICRDPYVHFPSVCQKLYF 81

Query: 73  LILKIYPASYKRRESQILEVEKKIGFFSPQFD-----SLACGSQAGM----KVEHLEDSA 132
           L+ K+YP ++K+RE Q+L+ E++   FSPQ D     S+  G    +    KVE   ++A
Sbjct: 82  LLKKMYPLAHKKREEQVLKEEQERECFSPQIDLVLDLSVCSGDSLNVSDKQKVEECSNAA 141

Query: 133 NGELNTNTKNDDAVAELILEENSDVVSSTSVVSLNSLQDPCAQKTQNQEKISVADVLCQA 192
           N   +++++ D       + +N +    T   +LN  ++   +  +  ++IS  D+LC A
Sbjct: 142 NLLSSSSSRGDIP----CIPKNQE---PTDAKALNVHENELLKDNKVSKQISKDDLLCSA 201

Query: 193 CTQLLFRPVVMNCGHVFCESCI---NSQVETLECQVCQSLQPRGFRNVCLELDQFLKEKF 252
           C +LL RPVV+NCGHV+CE C+     + E ++CQ C    PRGF  VCL L+Q L+E F
Sbjct: 202 CKELLVRPVVLNCGHVYCEGCVVDMAEESEKIKCQECNVCDPRGFPKVCLILEQLLEENF 261

Query: 253 PEEYSIRRDSVQLKLANSMKND------NPTSCSNEEGKKGQYLPRWDDVASKVHTFIGC 312
           PEEY+ R   VQ  LA++ K +         S SN+      +L    +  S VH   GC
Sbjct: 262 PEEYNSRSSKVQKTLAHNSKGNIQSYLKEGPSLSNDNNNDDPWLA---NPGSNVHFGAGC 321

Query: 313 DYCGMFPLIGDRYKCKDCLEASGFDLCGDCYNTRSKRPGRFNQQHRPEHRFQL 348
           D CG++P+IGDRY+CKDC E  G+DLC DCY T SK PGRFNQQH P+HR +L
Sbjct: 322 DSCGVYPIIGDRYRCKDCKEEIGYDLCKDCYETPSKVPGRFNQQHTPDHRLEL 364

[Close]

BLAST of CSPI07G14180 vs. ExPASy Swiss-Prot
Match: Q1L5Z9 (LON peptidase N-terminal domain and RING finger protein 2 OS=Homo sapiens OX=9606 GN=LONRF2 PE=1 SV=3)

HSP 1 Score: 53.5 bits (127), Expect = 5.6e-06
Identity = 19/48 (39.58%), Postives = 31/48 (64.58%), Query Frame = 0


Query: 10  VDADAFLCCVCLDLLYKPIVLPCGHISCFWCVHKCMNGFHESHCPICR 58
           +D   F C +C+ LL++P+  PCGH  C  C+ +C++  H  HCP+C+
Sbjct: 442 LDVTDFECALCMRLLFEPVTTPCGHTFCLKCLERCLD--HAPHCPLCK 487

[Close]

BLAST of CSPI07G14180 vs. ExPASy Swiss-Prot
Match: Q496Y0 (LON peptidase N-terminal domain and RING finger protein 3 OS=Homo sapiens OX=9606 GN=LONRF3 PE=1 SV=1)

HSP 1 Score: 53.5 bits (127), Expect = 5.6e-06
Identity = 30/111 (27.03%), Postives = 48/111 (43.24%), Query Frame = 0


Query: 7   LDDVDADAFLCCVCLDLLYKPIVLPCGHISCFWCVHKCMNGFHESHCPICR--------- 66
           L   DA    C +C+ L Y+P+  PCGH  C  C+ +C++  H + CP+C+         
Sbjct: 457 LASFDASDLECALCMRLFYEPVTTPCGHTFCLKCLERCLD--HNAKCPLCKDGLSQCLAS 516

Query: 67  RSYYHFPTICEILHQLILKIYPASYKRRESQILEVEKKIGFFSPQFDSLAC 109
           R Y    +   I+ +LI K  P   K R     E  +++   +       C
Sbjct: 517 RKY----SKNVIMEELIAKFLPEELKERRKLYEEEMEELSNLNKNVPIFVC 561

[Close]

BLAST of CSPI07G14180 vs. ExPASy Swiss-Prot
Match: Q8HXH0 (LON peptidase N-terminal domain and RING finger protein 3 OS=Macaca fascicularis OX=9541 GN=LONRF3 PE=2 SV=1)

HSP 1 Score: 53.5 bits (127), Expect = 5.6e-06
Identity = 30/111 (27.03%), Postives = 48/111 (43.24%), Query Frame = 0


Query: 7   LDDVDADAFLCCVCLDLLYKPIVLPCGHISCFWCVHKCMNGFHESHCPICR--------- 66
           L   DA    C +C+ L Y+P+  PCGH  C  C+ +C++  H + CP+C+         
Sbjct: 416 LASFDASDLECALCMRLFYEPVTTPCGHTFCLKCLERCLD--HNAKCPLCKDGLSQCLAS 475

Query: 67  RSYYHFPTICEILHQLILKIYPASYKRRESQILEVEKKIGFFSPQFDSLAC 109
           R Y    +   I+ +LI K  P   K R     E  +++   +       C
Sbjct: 476 RKY----SKNVIMEELIAKFLPEELKERRKLYEEEMEELSNLNKNVPIFVC 520

[Close]

BLAST of CSPI07G14180 vs. ExPASy Swiss-Prot
Match: Q9D4H7 (LON peptidase N-terminal domain and RING finger protein 3 OS=Mus musculus OX=10090 GN=Lonrf3 PE=2 SV=1)

HSP 1 Score: 53.1 bits (126), Expect = 7.4e-06
Identity = 28/89 (31.46%), Postives = 43/89 (48.31%), Query Frame = 0


Query: 7   LDDVDADAFLCCVCLDLLYKPIVLPCGHISCFWCVHKCMNGFHESHCPICR--------- 66
           L   DA    C +C+ L Y+P+  PCGH  C  C+ +C++  H + CP+C+         
Sbjct: 451 LGSFDASDLECSLCMRLFYEPVTTPCGHTFCLKCLERCLD--HNAKCPLCKDVLLQCLPS 510

Query: 67  RSYYHFPTICEILHQLILKIYPASYKRRE 87
           R Y    +   IL +LI    P  +K R+
Sbjct: 511 RKY----SKNVILEELIATFLPEEFKERK 533

[Close]

BLAST of CSPI07G14180 vs. ExPASy TrEMBL
Match: A0A0A0K4I8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G339130 PE=4 SV=1)

HSP 1 Score: 745.3 bits (1923), Expect = 1.2e-211
Identity = 354/359 (98.61%), Postives = 357/359 (99.44%), Query Frame = 0


Query: 1   MAEYQMLDDVDADAFLCCVCLDLLYKPIVLPCGHISCFWCVHKCMNGFHESHCPICRRSY 60
           MAEYQMLDDVDADAFLCCVCLDLLYKPIVLPCGHISCFWCVHKCMNGF ESHCPICRRSY
Sbjct: 1   MAEYQMLDDVDADAFLCCVCLDLLYKPIVLPCGHISCFWCVHKCMNGFRESHCPICRRSY 60

Query: 61  YHFPTICEILHQLILKIYPASYKRRESQILEVEKKIGFFSPQFDSLACGSQAGMKVEHLE 120
           YHFPTICEILHQLILKIYPASYKRRESQILEVEKKIGFFSPQFDSLACGSQAGMKVEHLE
Sbjct: 61  YHFPTICEILHQLILKIYPASYKRRESQILEVEKKIGFFSPQFDSLACGSQAGMKVEHLE 120

Query: 121 DSANGELNTNTKNDDAVAELILEENSDVVSSTSVVSLNSLQDPCAQKTQNQEKISVADVL 180
           DSANGELNTNTKNDDAVAELILEENSDVVSSTSVVSLNSLQDPCAQKTQNQEKISVADVL
Sbjct: 121 DSANGELNTNTKNDDAVAELILEENSDVVSSTSVVSLNSLQDPCAQKTQNQEKISVADVL 180

Query: 181 CQACTQLLFRPVVMNCGHVFCESCINSQVETLECQVCQSLQPRGFRNVCLELDQFLKEKF 240
           CQACTQLLFRPVVMNCGHVFCESCINSQVETLECQVCQSLQPRGFRNVCLELDQFLKEKF
Sbjct: 181 CQACTQLLFRPVVMNCGHVFCESCINSQVETLECQVCQSLQPRGFRNVCLELDQFLKEKF 240

Query: 241 PEEYSIRRDSVQLKLANSMKNDNPTSCSNEEGKKGQYLPRWDDVASKVHTFIGCDYCGMF 300
           PEEYSIRRDSVQLKLANSMK+DNPTSCSNEEGKKG+YLPRW DVASKVHTFIGCDYCGMF
Sbjct: 241 PEEYSIRRDSVQLKLANSMKHDNPTSCSNEEGKKGEYLPRWGDVASKVHTFIGCDYCGMF 300

Query: 301 PLIGDRYKCKDCLEASGFDLCGDCYNTRSKRPGRFNQQHRPEHRFQLVHPSMFQNMTQG 360
           PLIGDRYKCKDCLEASGFDLCGDCYNTRSKRPGRFNQQHRPEHRFQLVHPSMFQNMT+G
Sbjct: 301 PLIGDRYKCKDCLEASGFDLCGDCYNTRSKRPGRFNQQHRPEHRFQLVHPSMFQNMTEG 359

[Close]

BLAST of CSPI07G14180 vs. ExPASy TrEMBL
Match: A0A1S3C815 (E3 ubiquitin-protein ligase PRT1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103497542 PE=4 SV=1)

HSP 1 Score: 679.5 bits (1752), Expect = 7.8e-192
Identity = 325/363 (89.53%), Postives = 339/363 (93.39%), Query Frame = 0


Query: 1   MAEYQMLDDVDAD----AFLCCVCLDLLYKPIVLPCGHISCFWCVHKCMNGFHESHCPIC 60
           MAEYQMLDDVDAD    AFLCCVCLDLL+KPIVL CGHISCFWCVHK MNGF ESHCPIC
Sbjct: 76  MAEYQMLDDVDADDIPHAFLCCVCLDLLHKPIVLSCGHISCFWCVHKSMNGFRESHCPIC 135

Query: 61  RRSYYHFPTICEILHQLILKIYPASYKRRESQILEVEKKIGFFSPQFDSLACGSQAGMKV 120
           RR YYHFPTICEILHQLI+KIYP +YKRRE+QILE EKKIG+FSPQFDSLACGSQAGMKV
Sbjct: 136 RRPYYHFPTICEILHQLIVKIYPTAYKRREAQILEEEKKIGYFSPQFDSLACGSQAGMKV 195

Query: 121 EHLEDSANGELNTNTKNDDAVAELILEENSDVVSSTSVVSLNSLQDPCAQKTQNQEKISV 180
           EHLEDSANGELNTNTKN DA+AELILEENSDVVSSTSVVSLNSLQDPCAQKTQ QEKISV
Sbjct: 196 EHLEDSANGELNTNTKNGDAIAELILEENSDVVSSTSVVSLNSLQDPCAQKTQKQEKISV 255

Query: 181 ADVLCQACTQLLFRPVVMNCGHVFCESCINSQVETLECQVCQSLQPRGFRNVCLELDQFL 240
           ADVLCQACTQLLFRPVV+NCGHVFCESCINSQVETLECQVCQSLQPRGF NVCLELDQFL
Sbjct: 256 ADVLCQACTQLLFRPVVINCGHVFCESCINSQVETLECQVCQSLQPRGFPNVCLELDQFL 315

Query: 241 KEKFPEEYSIRRDSVQLKLANSMKNDNPTSCSNEEGKKGQYLPRWDDVASKVHTFIGCDY 300
           KE+FPEEYSIRRDSVQL+ ANS+K+DNPTSCS EEGKKGQYLPRW DVASKVH+ +GCD 
Sbjct: 316 KEQFPEEYSIRRDSVQLEQANSIKHDNPTSCSIEEGKKGQYLPRWGDVASKVHSAVGCDS 375

Query: 301 CGMFPLIGDRYKCKDCLEASGFDLCGDCYNTRSKRPGRFNQQHRPEHRFQLVHPSMFQNM 360
           CGMFPLIGDRYKCKDCLE  GFDLCGDCYNTRSKRPGRFNQQHRPEHRF LVHP MF+NM
Sbjct: 376 CGMFPLIGDRYKCKDCLEEVGFDLCGDCYNTRSKRPGRFNQQHRPEHRFHLVHPPMFRNM 435

[Close]

BLAST of CSPI07G14180 vs. ExPASy TrEMBL
Match: A0A6J1KS10 (E3 ubiquitin-protein ligase PRT1 OS=Cucurbita maxima OX=3661 GN=LOC111498135 PE=4 SV=1)

HSP 1 Score: 589.3 bits (1518), Expect = 1.1e-164
Identity = 276/360 (76.67%), Postives = 313/360 (86.94%), Query Frame = 0


Query: 1   MAEYQMLDDVDAD----AFLCCVCLDLLYKPIVLPCGHISCFWCVHKCMNGFHESHCPIC 60
           MA+ +MLDDVD+D    AFLCCVCLDLLYKPIVL CGHISCFWCVHK MNGF ESHCPIC
Sbjct: 34  MADSKMLDDVDSDDIPHAFLCCVCLDLLYKPIVLSCGHISCFWCVHKSMNGFRESHCPIC 93

Query: 61  RRSYYHFPTICEILHQLILKIYPASYKRRESQILEVEKKIGFFSPQFDSLACGSQAGMKV 120
           RR YYHFPTICE+LH LILK+YP +YKRR++Q LE E+K+G FSPQ+DSLACGS+AG K+
Sbjct: 94  RRPYYHFPTICEMLHLLILKMYPMAYKRRQNQTLEEERKLGLFSPQYDSLACGSRAGEKM 153

Query: 121 EHLEDSANGELNTNTKNDDAVAELILEENSDVVSSTSVVSLNSLQDPCAQKTQNQEKISV 180
           EHLEDSANGE+N NTKND+ VAELILEENS+VV S SV  LNSL D   QKTQNQE ++V
Sbjct: 154 EHLEDSANGEMNANTKNDNVVAELILEENSEVVRSASVEILNSLGDLHGQKTQNQETVTV 213

Query: 181 ADVLCQACTQLLFRPVVMNCGHVFCESCINSQVETLECQVCQSLQPRGFRNVCLELDQFL 240
           ADVLC+ACTQLL RPVV+NCGH FCESCIN +VETLECQVCQSLQPRGF NVCLELDQFL
Sbjct: 214 ADVLCRACTQLLLRPVVLNCGHAFCESCINLRVETLECQVCQSLQPRGFPNVCLELDQFL 273

Query: 241 KEKFPEEYSIRRDSVQLKLANSMKNDNPTSCSNEEGKKGQYLPRWDDVASKVHTFIGCDY 300
           KE+ PEEY +R DSVQLKLA + K+++PTSCS+E G KGQ LPRWD+VASKVH  +GCD 
Sbjct: 274 KEQLPEEYKLRSDSVQLKLAETFKHESPTSCSSEGGMKGQGLPRWDEVASKVHPAVGCDS 333

Query: 301 CGMFPLIGDRYKCKDCLEASGFDLCGDCYNTRSKRPGRFNQQHRPEHRFQLVHPSMFQNM 357
           CGM+P+IGDRYKC+DC E  GFDLCG+CYNTRSKRPGRFNQQHRPEH+F+LV P+MF  M
Sbjct: 334 CGMYPIIGDRYKCEDCFEEVGFDLCGECYNTRSKRPGRFNQQHRPEHKFKLVFPTMFSTM 393

[Close]

BLAST of CSPI07G14180 vs. ExPASy TrEMBL
Match: A0A6J1HAU1 (E3 ubiquitin-protein ligase PRT1 OS=Cucurbita moschata OX=3662 GN=LOC111461119 PE=4 SV=1)

HSP 1 Score: 587.8 bits (1514), Expect = 3.1e-164
Identity = 277/361 (76.73%), Postives = 314/361 (86.98%), Query Frame = 0


Query: 1   MAEYQMLDDVDAD----AFLCCVCLDLLYKPIVLPCGHISCFWCVHKCMNGFHESHCPIC 60
           MA+ +MLDDVD+D    AFLCCVCLDLLYKPIVL CGHISCFWCVHK MNGF ESHCPIC
Sbjct: 34  MADSKMLDDVDSDDIPHAFLCCVCLDLLYKPIVLSCGHISCFWCVHKSMNGFRESHCPIC 93

Query: 61  RRSYYHFPTICEILHQLILKIYPASYKRRESQILEVEKKIGFFSPQFDSLACGSQAGMKV 120
           RR YYHFPTICE+LH LILK+YP +YKRR++Q LE E+K+G FSPQ+DSLACGS+AG K+
Sbjct: 94  RRPYYHFPTICEMLHLLILKMYPMAYKRRQNQTLEEERKLGLFSPQYDSLACGSRAGEKI 153

Query: 121 EHLEDSANGELNTNTKNDDAVAELILEENSDVVSSTSVVSLNSLQDPCAQKTQNQEKISV 180
           EHLEDSANGE+N NTKND+ VAELILEENS+VV S SV  LNSL D   Q TQNQE ++V
Sbjct: 154 EHLEDSANGEMNANTKNDNVVAELILEENSEVVRSASVEILNSLGDLHGQNTQNQETVTV 213

Query: 181 ADVLCQACTQLLFRPVVMNCGHVFCESCINSQVETLECQVCQSLQPRGFRNVCLELDQFL 240
           ADVLC+ACTQLL RPVV+NCGH FCESCIN +VETLECQVCQSLQPRGF NVCLELDQFL
Sbjct: 214 ADVLCRACTQLLLRPVVLNCGHAFCESCINLRVETLECQVCQSLQPRGFPNVCLELDQFL 273

Query: 241 KEKFPEEYSIRRDSVQLKLANSMKNDNPTSCSNEE-GKKGQYLPRWDDVASKVHTFIGCD 300
           KE+ PEEY +R DSVQLKLA + K+++PTSCS+EE GKKGQ LPRWD+VASKVH  +GCD
Sbjct: 274 KEQLPEEYKLRSDSVQLKLAETFKHESPTSCSSEEGGKKGQGLPRWDEVASKVHPAVGCD 333

Query: 301 YCGMFPLIGDRYKCKDCLEASGFDLCGDCYNTRSKRPGRFNQQHRPEHRFQLVHPSMFQN 357
            CGM+P+IGDRYKC+DC E  GFDLCG+CYNTRSKRPGRFNQQHRPEH+F+LV P+MF  
Sbjct: 334 SCGMYPIIGDRYKCEDCFEEVGFDLCGECYNTRSKRPGRFNQQHRPEHKFKLVLPTMFST 393

[Close]

BLAST of CSPI07G14180 vs. ExPASy TrEMBL
Match: A0A6J1D209 (E3 ubiquitin-protein ligase PRT1 isoform X3 OS=Momordica charantia OX=3673 GN=LOC111016854 PE=4 SV=1)

HSP 1 Score: 540.4 bits (1391), Expect = 5.6e-150
Identity = 262/362 (72.38%), Postives = 293/362 (80.94%), Query Frame = 0


Query: 1   MAEYQMLDDVDAD----AFLCCVCLDLLYKPIVLPCGHISCFWCVHKCMNGFHESHCPIC 60
           MA+ Q+LDDVD +    AFLCCVCLDLL+KPIVL CGHISCFWCVHK M+GF ESHCPIC
Sbjct: 1   MADDQLLDDVDCNDVPHAFLCCVCLDLLHKPIVLSCGHISCFWCVHKSMSGFRESHCPIC 60

Query: 61  RRSYYHFPTICEILHQLILKIYPASYKRRESQILEVEKKIGFFSPQFDSLACGSQAGMKV 120
           R  YYHFPTICE+LH LILKI+P +YK+RE+QILE EK +G+FSPQFD+LAC SQAG KV
Sbjct: 61  RHPYYHFPTICEMLHLLILKIFPVAYKKRENQILEEEKIMGYFSPQFDALACDSQAGKKV 120

Query: 121 EHLEDSANGELNTNTKNDDAVAELILEENSDVVSSTSVVSLNSLQDPCAQKTQNQEKISV 180
           EH EDSANGEL TNT ND  VAE ILEENS VV             P  QKTQN EK+SV
Sbjct: 121 EHSEDSANGELKTNTNNDKVVAERILEENSAVVG------------PYGQKTQNHEKVSV 180

Query: 181 ADVLCQACTQLLFRPVVMNCGHVFCESCINSQVETLECQVCQSLQPRGFRNVCLELDQFL 240
           AD+LC+ACTQLLFRPVV+NCGH  CESCIN QVETL+CQVC+ LQP GF NVCL LDQFL
Sbjct: 181 ADLLCRACTQLLFRPVVLNCGHALCESCINLQVETLKCQVCECLQPSGFPNVCLMLDQFL 240

Query: 241 KEKFPEEYSIRRDSVQLKLANSMKNDNPTSCSNEEGKKGQYLPRWDDVASKVHTFIGCDY 300
           KE+FPEEYS+RRDSVQLKLAN  K ++PTSCS+E GKKG+ L R  +VASKVH   GCD 
Sbjct: 241 KEQFPEEYSLRRDSVQLKLANIFKRESPTSCSDERGKKGEDLSRSGEVASKVHYGAGCDS 300

Query: 301 CGMFPLIGDRYKCKDCLEASGFDLCGDCYNTRSKRPGRFNQQHRPEHRFQLVHPS--MFQ 357
           CGM P++GDRYKCKDC E  GFDLCGDCYNT SKRPGRFNQQHRPEH F+L+ PS  MF 
Sbjct: 301 CGMCPIVGDRYKCKDCYEKIGFDLCGDCYNTCSKRPGRFNQQHRPEHNFELIGPSNVMFP 350

[Close]

BLAST of CSPI07G14180 vs. NCBI nr
Match: XP_004139635.1 (E3 ubiquitin-protein ligase PRT1 [Cucumis sativus] >KGN44590.1 hypothetical protein Csa_015910 [Cucumis sativus])

HSP 1 Score: 745.3 bits (1923), Expect = 2.4e-211
Identity = 354/359 (98.61%), Postives = 357/359 (99.44%), Query Frame = 0


Query: 1   MAEYQMLDDVDADAFLCCVCLDLLYKPIVLPCGHISCFWCVHKCMNGFHESHCPICRRSY 60
           MAEYQMLDDVDADAFLCCVCLDLLYKPIVLPCGHISCFWCVHKCMNGF ESHCPICRRSY
Sbjct: 1   MAEYQMLDDVDADAFLCCVCLDLLYKPIVLPCGHISCFWCVHKCMNGFRESHCPICRRSY 60

Query: 61  YHFPTICEILHQLILKIYPASYKRRESQILEVEKKIGFFSPQFDSLACGSQAGMKVEHLE 120
           YHFPTICEILHQLILKIYPASYKRRESQILEVEKKIGFFSPQFDSLACGSQAGMKVEHLE
Sbjct: 61  YHFPTICEILHQLILKIYPASYKRRESQILEVEKKIGFFSPQFDSLACGSQAGMKVEHLE 120

Query: 121 DSANGELNTNTKNDDAVAELILEENSDVVSSTSVVSLNSLQDPCAQKTQNQEKISVADVL 180
           DSANGELNTNTKNDDAVAELILEENSDVVSSTSVVSLNSLQDPCAQKTQNQEKISVADVL
Sbjct: 121 DSANGELNTNTKNDDAVAELILEENSDVVSSTSVVSLNSLQDPCAQKTQNQEKISVADVL 180

Query: 181 CQACTQLLFRPVVMNCGHVFCESCINSQVETLECQVCQSLQPRGFRNVCLELDQFLKEKF 240
           CQACTQLLFRPVVMNCGHVFCESCINSQVETLECQVCQSLQPRGFRNVCLELDQFLKEKF
Sbjct: 181 CQACTQLLFRPVVMNCGHVFCESCINSQVETLECQVCQSLQPRGFRNVCLELDQFLKEKF 240

Query: 241 PEEYSIRRDSVQLKLANSMKNDNPTSCSNEEGKKGQYLPRWDDVASKVHTFIGCDYCGMF 300
           PEEYSIRRDSVQLKLANSMK+DNPTSCSNEEGKKG+YLPRW DVASKVHTFIGCDYCGMF
Sbjct: 241 PEEYSIRRDSVQLKLANSMKHDNPTSCSNEEGKKGEYLPRWGDVASKVHTFIGCDYCGMF 300

Query: 301 PLIGDRYKCKDCLEASGFDLCGDCYNTRSKRPGRFNQQHRPEHRFQLVHPSMFQNMTQG 360
           PLIGDRYKCKDCLEASGFDLCGDCYNTRSKRPGRFNQQHRPEHRFQLVHPSMFQNMT+G
Sbjct: 301 PLIGDRYKCKDCLEASGFDLCGDCYNTRSKRPGRFNQQHRPEHRFQLVHPSMFQNMTEG 359

[Close]

BLAST of CSPI07G14180 vs. NCBI nr
Match: XP_008457982.1 (PREDICTED: E3 ubiquitin-protein ligase PRT1 isoform X1 [Cucumis melo])

HSP 1 Score: 679.5 bits (1752), Expect = 1.6e-191
Identity = 325/363 (89.53%), Postives = 339/363 (93.39%), Query Frame = 0


Query: 1   MAEYQMLDDVDAD----AFLCCVCLDLLYKPIVLPCGHISCFWCVHKCMNGFHESHCPIC 60
           MAEYQMLDDVDAD    AFLCCVCLDLL+KPIVL CGHISCFWCVHK MNGF ESHCPIC
Sbjct: 76  MAEYQMLDDVDADDIPHAFLCCVCLDLLHKPIVLSCGHISCFWCVHKSMNGFRESHCPIC 135

Query: 61  RRSYYHFPTICEILHQLILKIYPASYKRRESQILEVEKKIGFFSPQFDSLACGSQAGMKV 120
           RR YYHFPTICEILHQLI+KIYP +YKRRE+QILE EKKIG+FSPQFDSLACGSQAGMKV
Sbjct: 136 RRPYYHFPTICEILHQLIVKIYPTAYKRREAQILEEEKKIGYFSPQFDSLACGSQAGMKV 195

Query: 121 EHLEDSANGELNTNTKNDDAVAELILEENSDVVSSTSVVSLNSLQDPCAQKTQNQEKISV 180
           EHLEDSANGELNTNTKN DA+AELILEENSDVVSSTSVVSLNSLQDPCAQKTQ QEKISV
Sbjct: 196 EHLEDSANGELNTNTKNGDAIAELILEENSDVVSSTSVVSLNSLQDPCAQKTQKQEKISV 255

Query: 181 ADVLCQACTQLLFRPVVMNCGHVFCESCINSQVETLECQVCQSLQPRGFRNVCLELDQFL 240
           ADVLCQACTQLLFRPVV+NCGHVFCESCINSQVETLECQVCQSLQPRGF NVCLELDQFL
Sbjct: 256 ADVLCQACTQLLFRPVVINCGHVFCESCINSQVETLECQVCQSLQPRGFPNVCLELDQFL 315

Query: 241 KEKFPEEYSIRRDSVQLKLANSMKNDNPTSCSNEEGKKGQYLPRWDDVASKVHTFIGCDY 300
           KE+FPEEYSIRRDSVQL+ ANS+K+DNPTSCS EEGKKGQYLPRW DVASKVH+ +GCD 
Sbjct: 316 KEQFPEEYSIRRDSVQLEQANSIKHDNPTSCSIEEGKKGQYLPRWGDVASKVHSAVGCDS 375

Query: 301 CGMFPLIGDRYKCKDCLEASGFDLCGDCYNTRSKRPGRFNQQHRPEHRFQLVHPSMFQNM 360
           CGMFPLIGDRYKCKDCLE  GFDLCGDCYNTRSKRPGRFNQQHRPEHRF LVHP MF+NM
Sbjct: 376 CGMFPLIGDRYKCKDCLEEVGFDLCGDCYNTRSKRPGRFNQQHRPEHRFHLVHPPMFRNM 435

[Close]

BLAST of CSPI07G14180 vs. NCBI nr
Match: XP_038877741.1 (E3 ubiquitin-protein ligase PRT1 isoform X1 [Benincasa hispida])

HSP 1 Score: 638.6 bits (1646), Expect = 3.1e-179
Identity = 305/363 (84.02%), Postives = 327/363 (90.08%), Query Frame = 0


Query: 1   MAEYQMLDDVDAD----AFLCCVCLDLLYKPIVLPCGHISCFWCVHKCMNGFHESHCPIC 60
           MA+ QMLDDVDAD    AFLC VCLDLLYKPIVL CGHISCFWCVHK MNGF ESHCPIC
Sbjct: 34  MADSQMLDDVDADDIPHAFLCGVCLDLLYKPIVLSCGHISCFWCVHKSMNGFRESHCPIC 93

Query: 61  RRSYYHFPTICEILHQLILKIYPASYKRRESQILEVEKKIGFFSPQFDSLACGSQAGMKV 120
           R  YYHFPTICE+LHQLILKIYPA YK+RE+QILE E+KIGFFSPQFDSLACGSQAG+KV
Sbjct: 94  RHPYYHFPTICEMLHQLILKIYPAVYKKRENQILEEERKIGFFSPQFDSLACGSQAGIKV 153

Query: 121 EHLEDSANGELNTNTKNDDAVAELILEENSDVVSSTSVVSLNSLQDPCAQKTQNQEKISV 180
           EHLEDSANGELNT TKNDD V ELILEE S+VVSS+SV SLNSLQDPCAQKTQNQEKISV
Sbjct: 154 EHLEDSANGELNTITKNDDVVTELILEETSEVVSSSSVKSLNSLQDPCAQKTQNQEKISV 213

Query: 181 ADVLCQACTQLLFRPVVMNCGHVFCESCINSQVETLECQVCQSLQPRGFRNVCLELDQFL 240
           ADVLCQACTQLLFRPVV+NCGH FCESCINSQVETLECQVCQ +QPRGF NVCLELDQFL
Sbjct: 214 ADVLCQACTQLLFRPVVLNCGHAFCESCINSQVETLECQVCQFVQPRGFPNVCLELDQFL 273

Query: 241 KEKFPEEYSIRRDSVQLKLANSMKNDNPTSCSNEEGKKGQYLPRWDDVASKVHTFIGCDY 300
           KE+FPEEYS+RRDS+QL LA S+K+D+P SCSNEEGKKG+ LPRW +VASKVH  +GCD 
Sbjct: 274 KEQFPEEYSLRRDSIQLVLAKSIKHDSPISCSNEEGKKGKDLPRWGEVASKVHYGVGCDS 333

Query: 301 CGMFPLIGDRYKCKDCLEASGFDLCGDCYNTRSKRPGRFNQQHRPEHRFQLVHPSMFQNM 360
           CGM+P+IGDRYKCKDCLE  GFDLCGDCYNTRSKRPGRFNQQHRPEH+FQLV P MF NM
Sbjct: 334 CGMYPIIGDRYKCKDCLEEIGFDLCGDCYNTRSKRPGRFNQQHRPEHKFQLVRPQMFHNM 393

[Close]

BLAST of CSPI07G14180 vs. NCBI nr
Match: XP_023515020.1 (E3 ubiquitin-protein ligase PRT1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 589.7 bits (1519), Expect = 1.7e-164
Identity = 276/360 (76.67%), Postives = 313/360 (86.94%), Query Frame = 0


Query: 1   MAEYQMLDDVDAD----AFLCCVCLDLLYKPIVLPCGHISCFWCVHKCMNGFHESHCPIC 60
           MA+ +MLDDVD+D    AFLCCVCLDLLYKPIVL CGHISCFWCVHK MNGF ESHCPIC
Sbjct: 34  MADSKMLDDVDSDDIPHAFLCCVCLDLLYKPIVLSCGHISCFWCVHKSMNGFRESHCPIC 93

Query: 61  RRSYYHFPTICEILHQLILKIYPASYKRRESQILEVEKKIGFFSPQFDSLACGSQAGMKV 120
           RR YYHFPTICE+LH LILK+YP +YKRR++Q LE E+K+G FSPQ+DSLACGS+AG K+
Sbjct: 94  RRPYYHFPTICEMLHLLILKMYPMAYKRRQNQTLEEERKLGLFSPQYDSLACGSRAGEKI 153

Query: 121 EHLEDSANGELNTNTKNDDAVAELILEENSDVVSSTSVVSLNSLQDPCAQKTQNQEKISV 180
           EHLEDSANGE+N NTKND+ VAELILEENS+VV S SV  LNSL D   Q TQNQE ++V
Sbjct: 154 EHLEDSANGEMNANTKNDNVVAELILEENSEVVRSASVEILNSLGDLHGQNTQNQETVTV 213

Query: 181 ADVLCQACTQLLFRPVVMNCGHVFCESCINSQVETLECQVCQSLQPRGFRNVCLELDQFL 240
           ADVLC+ACTQLL RPVV+NCGH FCESCIN +VETLECQVCQSLQPRGF NVCLELDQFL
Sbjct: 214 ADVLCRACTQLLLRPVVLNCGHAFCESCINLRVETLECQVCQSLQPRGFPNVCLELDQFL 273

Query: 241 KEKFPEEYSIRRDSVQLKLANSMKNDNPTSCSNEEGKKGQYLPRWDDVASKVHTFIGCDY 300
           KE+ PEEY +R DSVQLKLA + K+++PTSCS+E GKKGQ LPRWD+VASKVH  +GCD 
Sbjct: 274 KEQLPEEYKLRSDSVQLKLAETFKHESPTSCSSEGGKKGQGLPRWDEVASKVHPAVGCDS 333

Query: 301 CGMFPLIGDRYKCKDCLEASGFDLCGDCYNTRSKRPGRFNQQHRPEHRFQLVHPSMFQNM 357
           CGM+P+IGDRYKC+DC E  GFDLCG+CYNTRSKRPGRFNQQHRPEH+F+LV P+MF  M
Sbjct: 334 CGMYPIIGDRYKCEDCFEEVGFDLCGECYNTRSKRPGRFNQQHRPEHKFKLVLPTMFSTM 393

[Close]

BLAST of CSPI07G14180 vs. NCBI nr
Match: XP_023005016.1 (E3 ubiquitin-protein ligase PRT1 [Cucurbita maxima] >XP_023005017.1 E3 ubiquitin-protein ligase PRT1 [Cucurbita maxima])

HSP 1 Score: 589.3 bits (1518), Expect = 2.2e-164
Identity = 276/360 (76.67%), Postives = 313/360 (86.94%), Query Frame = 0


Query: 1   MAEYQMLDDVDAD----AFLCCVCLDLLYKPIVLPCGHISCFWCVHKCMNGFHESHCPIC 60
           MA+ +MLDDVD+D    AFLCCVCLDLLYKPIVL CGHISCFWCVHK MNGF ESHCPIC
Sbjct: 34  MADSKMLDDVDSDDIPHAFLCCVCLDLLYKPIVLSCGHISCFWCVHKSMNGFRESHCPIC 93

Query: 61  RRSYYHFPTICEILHQLILKIYPASYKRRESQILEVEKKIGFFSPQFDSLACGSQAGMKV 120
           RR YYHFPTICE+LH LILK+YP +YKRR++Q LE E+K+G FSPQ+DSLACGS+AG K+
Sbjct: 94  RRPYYHFPTICEMLHLLILKMYPMAYKRRQNQTLEEERKLGLFSPQYDSLACGSRAGEKM 153

Query: 121 EHLEDSANGELNTNTKNDDAVAELILEENSDVVSSTSVVSLNSLQDPCAQKTQNQEKISV 180
           EHLEDSANGE+N NTKND+ VAELILEENS+VV S SV  LNSL D   QKTQNQE ++V
Sbjct: 154 EHLEDSANGEMNANTKNDNVVAELILEENSEVVRSASVEILNSLGDLHGQKTQNQETVTV 213

Query: 181 ADVLCQACTQLLFRPVVMNCGHVFCESCINSQVETLECQVCQSLQPRGFRNVCLELDQFL 240
           ADVLC+ACTQLL RPVV+NCGH FCESCIN +VETLECQVCQSLQPRGF NVCLELDQFL
Sbjct: 214 ADVLCRACTQLLLRPVVLNCGHAFCESCINLRVETLECQVCQSLQPRGFPNVCLELDQFL 273

Query: 241 KEKFPEEYSIRRDSVQLKLANSMKNDNPTSCSNEEGKKGQYLPRWDDVASKVHTFIGCDY 300
           KE+ PEEY +R DSVQLKLA + K+++PTSCS+E G KGQ LPRWD+VASKVH  +GCD 
Sbjct: 274 KEQLPEEYKLRSDSVQLKLAETFKHESPTSCSSEGGMKGQGLPRWDEVASKVHPAVGCDS 333

Query: 301 CGMFPLIGDRYKCKDCLEASGFDLCGDCYNTRSKRPGRFNQQHRPEHRFQLVHPSMFQNM 357
           CGM+P+IGDRYKC+DC E  GFDLCG+CYNTRSKRPGRFNQQHRPEH+F+LV P+MF  M
Sbjct: 334 CGMYPIIGDRYKCEDCFEEVGFDLCGECYNTRSKRPGRFNQQHRPEHKFKLVFPTMFSTM 393

[Close]

BLAST of CSPI07G14180 vs. TAIR 10
Match: AT3G24800.1 (proteolysis 1 )

HSP 1 Score: 318.9 bits (816), Expect = 5.1e-87
Identity = 164/353 (46.46%), Postives = 222/353 (62.89%), Query Frame = 0


Query: 13  DAFLCCVCLDLLYKPIVLPCGHISCFWCVHKCMNGFHESHCPICRRSYYHFPTICEILHQ 72
           D FLCCVCL+LLYKPIVL CGH+SCFWCVHK MNGF ESHCPICR  Y HFP++C+ L+ 
Sbjct: 22  DQFLCCVCLELLYKPIVLSCGHLSCFWCVHKSMNGFRESHCPICRDPYVHFPSVCQKLYF 81

Query: 73  LILKIYPASYKRRESQILEVEKKIGFFSPQFD-----SLACGSQAGM----KVEHLEDSA 132
           L+ K+YP ++K+RE Q+L+ E++   FSPQ D     S+  G    +    KVE   ++A
Sbjct: 82  LLKKMYPLAHKKREEQVLKEEQERECFSPQIDLVLDLSVCSGDSLNVSDKQKVEECSNAA 141

Query: 133 NGELNTNTKNDDAVAELILEENSDVVSSTSVVSLNSLQDPCAQKTQNQEKISVADVLCQA 192
           N   +++++ D       + +N +    T   +LN  ++   +  +  ++IS  D+LC A
Sbjct: 142 NLLSSSSSRGDIP----CIPKNQE---PTDAKALNVHENELLKDNKVSKQISKDDLLCSA 201

Query: 193 CTQLLFRPVVMNCGHVFCESCI---NSQVETLECQVCQSLQPRGFRNVCLELDQFLKEKF 252
           C +LL RPVV+NCGHV+CE C+     + E ++CQ C    PRGF  VCL L+Q L+E F
Sbjct: 202 CKELLVRPVVLNCGHVYCEGCVVDMAEESEKIKCQECNVCDPRGFPKVCLILEQLLEENF 261

Query: 253 PEEYSIRRDSVQLKLANSMKND------NPTSCSNEEGKKGQYLPRWDDVASKVHTFIGC 312
           PEEY+ R   VQ  LA++ K +         S SN+      +L    +  S VH   GC
Sbjct: 262 PEEYNSRSSKVQKTLAHNSKGNIQSYLKEGPSLSNDNNNDDPWLA---NPGSNVHFGAGC 321

Query: 313 DYCGMFPLIGDRYKCKDCLEASGFDLCGDCYNTRSKRPGRFNQQHRPEHRFQL 348
           D CG++P+IGDRY+CKDC E  G+DLC DCY T SK PGRFNQQH P+HR +L
Sbjct: 322 DSCGVYPIIGDRYRCKDCKEEIGYDLCKDCYETPSKVPGRFNQQHTPDHRLEL 364

[Close]

BLAST of CSPI07G14180 vs. TAIR 10
Match: AT2G42030.1 (RING/U-box superfamily protein )

HSP 1 Score: 48.5 bits (114), Expect = 1.3e-05
Identity = 23/78 (29.49%), Postives = 39/78 (50.00%), Query Frame = 0


Query: 11  DADAFLCCVCLDLLYKPIVLPCGHISCFWCVHKCMNGFHESHCPICRRSYYHFPTICEIL 70
           D   F C +CLDL   P+V  CGH+ C+ C+++ +       CP+C+          E+ 
Sbjct: 135 DGSFFDCYICLDLSKDPVVTNCGHLYCWSCLYQWLQVSEAKECPVCKG---------EVS 194

Query: 71  HQLILKIYPASYKRRESQ 89
            + +  IY    ++RES+
Sbjct: 195 VKTVTPIYGRGIQKRESE 203

[Close]

BLAST of CSPI07G14180 vs. TAIR 10
Match: AT4G03510.1 (RING membrane-anchor 1 )

HSP 1 Score: 47.8 bits (112), Expect = 2.2e-05
Identity = 23/64 (35.94%), Postives = 34/64 (53.12%), Query Frame = 0


Query: 8   DDVDADAFLCCVCLDLLYKPIVLPCGHISCFWCVHKCMN--GFHES-------HCPICRR 63
           DD D   F C +CLD + +P+V  CGH+ C+ C+HK ++   F  S        CP+C+ 
Sbjct: 39  DDTDDSNFDCNICLDSVQEPVVTLCGHLFCWPCIHKWLDVQSFSTSDEYQRHRQCPVCKS 98

[Close]

BLAST of CSPI07G14180 vs. TAIR 10
Match: AT4G03510.2 (RING membrane-anchor 1 )

HSP 1 Score: 47.8 bits (112), Expect = 2.2e-05
Identity = 23/64 (35.94%), Postives = 34/64 (53.12%), Query Frame = 0


Query: 8   DDVDADAFLCCVCLDLLYKPIVLPCGHISCFWCVHKCMN--GFHES-------HCPICRR 63
           DD D   F C +CLD + +P+V  CGH+ C+ C+HK ++   F  S        CP+C+ 
Sbjct: 39  DDTDDSNFDCNICLDSVQEPVVTLCGHLFCWPCIHKWLDVQSFSTSDEYQRHRQCPVCKS 98

[Close]

BLAST of CSPI07G14180 vs. TAIR 10
Match: AT1G04020.2 (breast cancer associated RING 1 )

HSP 1 Score: 47.0 bits (110), Expect = 3.8e-05
Identity = 20/44 (45.45%), Postives = 29/44 (65.91%), Query Frame = 0


Query: 17 CCVCLDLLYKPIVLPCGHISCFWCVHKCMNGFHESHCPICRRSY 61
          C +CL LL +P++LPC H+ C  CVHK  +   ES CP+C+  +
Sbjct: 25 CPLCLKLLNRPVLLPCDHVFCDSCVHK--SSQVESGCPVCKSKH 66

The following BLAST results are available for this feature:

BLAST of CSPI07G14180 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8LBL5	7.2e-86	46.46	E3 ubiquitin-protein ligase PRT1 OS=Arabidopsis thaliana OX=3702 GN=PRT1 PE=2 SV...	[more]
Q1L5Z9	5.6e-06	39.58	LON peptidase N-terminal domain and RING finger protein 2 OS=Homo sapiens OX=960...	[more]
Q496Y0	5.6e-06	27.03	LON peptidase N-terminal domain and RING finger protein 3 OS=Homo sapiens OX=960...	[more]
Q8HXH0	5.6e-06	27.03	LON peptidase N-terminal domain and RING finger protein 3 OS=Macaca fascicularis...	[more]
Q9D4H7	7.4e-06	31.46	LON peptidase N-terminal domain and RING finger protein 3 OS=Mus musculus OX=100...	[more]

BLAST of CSPI07G14180 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K4I8	1.2e-211	98.61	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G339130 PE=4 SV=1	[more]
A0A1S3C815	7.8e-192	89.53	E3 ubiquitin-protein ligase PRT1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103497...	[more]
A0A6J1KS10	1.1e-164	76.67	E3 ubiquitin-protein ligase PRT1 OS=Cucurbita maxima OX=3661 GN=LOC111498135 PE=...	[more]
A0A6J1HAU1	3.1e-164	76.73	E3 ubiquitin-protein ligase PRT1 OS=Cucurbita moschata OX=3662 GN=LOC111461119 P...	[more]
A0A6J1D209	5.6e-150	72.38	E3 ubiquitin-protein ligase PRT1 isoform X3 OS=Momordica charantia OX=3673 GN=LO...	[more]

BLAST of CSPI07G14180 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004139635.1	2.4e-211	98.61	E3 ubiquitin-protein ligase PRT1 [Cucumis sativus] >KGN44590.1 hypothetical prot...	[more]
XP_008457982.1	1.6e-191	89.53	PREDICTED: E3 ubiquitin-protein ligase PRT1 isoform X1 [Cucumis melo]	[more]
XP_038877741.1	3.1e-179	84.02	E3 ubiquitin-protein ligase PRT1 isoform X1 [Benincasa hispida]	[more]
XP_023515020.1	1.7e-164	76.67	E3 ubiquitin-protein ligase PRT1 [Cucurbita pepo subsp. pepo]	[more]
XP_023005016.1	2.2e-164	76.67	E3 ubiquitin-protein ligase PRT1 [Cucurbita maxima] >XP_023005017.1 E3 ubiquitin...	[more]

BLAST of CSPI07G14180 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G24800.1	5.1e-87	46.46	proteolysis 1	[more]
AT2G42030.1	1.3e-05	29.49	RING/U-box superfamily protein	[more]
AT4G03510.1	2.2e-05	35.94	RING membrane-anchor 1	[more]
AT4G03510.2	2.2e-05	35.94	RING membrane-anchor 1	[more]
AT1G04020.2	3.8e-05	45.45	breast cancer associated RING 1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 17..56 e-value: 1.8E-4 score: 30.8 coord: 181..217 e-value: 2.8E-5 score: 33.6
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 181..218 score: 10.763177
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 17..57 score: 11.471321
IPR043145	Zinc finger, ZZ-type superfamily	GENE3D	3.30.60.90		coord: 283..348 e-value: 3.0E-17 score: 64.1
IPR027370	RING-type zinc-finger, LisH dimerisation motif	PFAM	PF13445	zf-RING_UBOX	coord: 181..208 e-value: 3.6E-6 score: 26.9
None	No IPR available	PFAM	PF13920	zf-C3HC4_3	coord: 15..60 e-value: 1.7E-8 score: 34.2
None	No IPR available	PANTHER	PTHR15898	UNCHARACTERIZED	coord: 12..352
None	No IPR available	PANTHER	PTHR15898:SF15	E3 UBIQUITIN-PROTEIN LIGASE PRT1-LIKE	coord: 12..352
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 12..66
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 175..221
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 287..348
IPR000433	Zinc finger, ZZ-type	PFAM	PF00569	ZZ	coord: 293..330 e-value: 4.8E-7 score: 29.5
IPR000433	Zinc finger, ZZ-type	PROSITE	PS50135	ZF_ZZ_2	coord: 288..343 score: 10.733705
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 129..247 e-value: 5.1E-11 score: 44.2
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 2..128 e-value: 4.5E-14 score: 54.4
IPR017907	Zinc finger, RING-type, conserved site	PROSITE	PS00518	ZF_RING_1	coord: 196..205

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI07G14180.1	CSPI07G14180.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043161	proteasome-mediated ubiquitin-dependent protein catabolic process
biological_process	GO:0016567	protein ubiquitination
molecular_function	GO:0061630	ubiquitin protein ligase activity
molecular_function	GO:0008270	zinc ion binding