CSPI07G12230 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI07G12230
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Ty3/gypsy retrotransposon protein
Location	Chr7: 10491882 .. 10492208 (+)
RNA-Seq Expression	CSPI07G12230
Synteny	CSPI07G12230

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCTTAGAGAGAAGTGAATCATTAGAAGAAGTAGAATCATGGGAGGAAGAATTAATAGTAGAAGAATCAGTAACTGTGATACTAAAAAGTTTTAAAGATGTCTTCGAGTGGCCCGAAACATTTCCTTCTGGAAGAGCAATTGAACACCATATTCACCTTAAAAAAGGAATGGATCTGGTGAATGTGAGACCATATCGTTATGCAAACCAACAGAAAACGGAAATGAAAAAGTTGGTAGAGGAAATGTTGTCATCAGGGGTAATCCGACCAAGTACAAGTCCTTATTCAAGCCATGTGTTGTTGGTAAGGAAGAAAGATGGAAGC

mRNA sequence

ATGGCCTTAGAGAGAAGTGAATCATTAGAAGAAGTAGAATCATGGGAGGAAGAATTAATAGTAGAAGAATCAGTAACTGTGATACTAAAAAGTTTTAAAGATGTCTTCGAGTGGCCCGAAACATTTCCTTCTGGAAGAGCAATTGAACACCATATTCACCTTAAAAAAGGAATGGATCTGGTGAATGTGAGACCATATCGTTATGCAAACCAACAGAAAACGGAAATGAAAAAGTTGGTAGAGGAAATGTTGTCATCAGGGGTAATCCGACCAAGTACAAGTCCTTATTCAAGCCATGTGTTGTTGGTAAGGAAGAAAGATGGAAGC

Coding sequence (CDS)

ATGGCCTTAGAGAGAAGTGAATCATTAGAAGAAGTAGAATCATGGGAGGAAGAATTAATAGTAGAAGAATCAGTAACTGTGATACTAAAAAGTTTTAAAGATGTCTTCGAGTGGCCCGAAACATTTCCTTCTGGAAGAGCAATTGAACACCATATTCACCTTAAAAAAGGAATGGATCTGGTGAATGTGAGACCATATCGTTATGCAAACCAACAGAAAACGGAAATGAAAAAGTTGGTAGAGGAAATGTTGTCATCAGGGGTAATCCGACCAAGTACAAGTCCTTATTCAAGCCATGTGTTGTTGGTAAGGAAGAAAGATGGAAGC

Protein sequence

MALERSESLEEVESWEEELIVEESVTVILKSFKDVFEWPETFPSGRAIEHHIHLKKGMDLVNVRPYRYANQQKTEMKKLVEEMLSSGVIRPSTSPYSSHVLLVRKKDGS

Homology

[Close]

BLAST of CSPI07G12230 vs. ExPASy Swiss-Prot
Match: Q99315 (Transposon Ty3-G Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY3B-G PE=1 SV=3)

HSP 1 Score: 53.1 bits (126), Expect = 2.2e-06
Identity = 25/62 (40.32%), Postives = 40/62 (64.52%), Query Frame = 0


Query: 48  IEHHIHLKKGMDLVNVRPYRYANQQKTEMKKLVEEMLSSGVIRPSTSPYSSHVLLVRKKD 107
           ++H I +K G  L  ++PY    + + E+ K+V+++L +  I PS SP SS V+LV KKD
Sbjct: 584 VKHDIEIKPGARLPRLQPYHVTEKNEQEINKIVQKLLDNKFIVPSKSPCSSPVVLVPKKD 643

Query: 108 GS 110
           G+
Sbjct: 644 GT 645

[Close]

BLAST of CSPI07G12230 vs. ExPASy Swiss-Prot
Match: Q7LHG5 (Transposon Ty3-I Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY3B-I PE=1 SV=2)

HSP 1 Score: 53.1 bits (126), Expect = 2.2e-06
Identity = 25/62 (40.32%), Postives = 40/62 (64.52%), Query Frame = 0


Query: 48  IEHHIHLKKGMDLVNVRPYRYANQQKTEMKKLVEEMLSSGVIRPSTSPYSSHVLLVRKKD 107
           ++H I +K G  L  ++PY    + + E+ K+V+++L +  I PS SP SS V+LV KKD
Sbjct: 610 VKHDIEIKPGARLPRLQPYHVTEKNEQEINKIVQKLLDNKFIVPSKSPCSSPVVLVPKKD 669

Query: 108 GS 110
           G+
Sbjct: 670 GT 671

[Close]

BLAST of CSPI07G12230 vs. ExPASy Swiss-Prot
Match: Q8I7P9 (Retrovirus-related Pol polyprotein from transposon opus OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 47.8 bits (112), Expect = 9.4e-05
Identity = 27/88 (30.68%), Postives = 48/88 (54.55%), Query Frame = 0


Query: 22  EESVTVILKSFKDVFEWPETFPSGRAIEHHIHLK---KGMDLVNVRPYRYANQQKTEMKK 81
           +E +  +L  F  +FE P    SG ++E  +  +      D +  + Y Y    + E+++
Sbjct: 85  QEILNSLLGEFPRIFEPP---LSGMSVETAVKAEIRTNTQDPIYAKSYPYPVNMRGEVER 144

Query: 82  LVEEMLSSGVIRPSTSPYSSHVLLVRKK 107
            ++E+L  G+IRPS SPY+S + +V KK
Sbjct: 145 QIDELLQDGIIRPSNSPYNSPIWIVPKK 169

[Close]

BLAST of CSPI07G12230 vs. ExPASy Swiss-Prot
Match: P92522 (Uncharacterized mitochondrial protein AtMg00850 OS=Arabidopsis thaliana OX=3702 GN=AtMg00850 PE=4 SV=1)

HSP 1 Score: 45.4 bits (106), Expect = 4.7e-04
Identity = 21/37 (56.76%), Postives = 29/37 (78.38%), Query Frame = 0


Query: 72  QKTEMKKLVEEMLSSGVIRPSTSPYSSHVLLVRKKDG 109
           ++T +K  + EML + +I+PS SPYSS VLLV+KKDG
Sbjct: 41  RRTRLKNWLGEMLEARIIQPSISPYSSPVLLVQKKDG 77

[Close]

BLAST of CSPI07G12230 vs. ExPASy TrEMBL
Match: A0A5A7TP42 (Ty3/gypsy retrotransposon protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G004030 PE=3 SV=1)

HSP 1 Score: 133.7 bits (335), Expect = 4.8e-28
Identity = 65/101 (64.36%), Postives = 79/101 (78.22%), Query Frame = 0


Query: 9   LEEVESWEEELIVEESVTVILKSFKDVFEWPETFPSGRAIEHHIHLKKGMDLVNVRPYRY 68
           LEE ++ E+  ++ E +  +LK F  VFEWP T P  R+IEHHI+LK G D VNVRPYRY
Sbjct: 861 LEEEQNQEQGKVIAEPIATLLKQFARVFEWPATLPPQRSIEHHIYLKSGTDPVNVRPYRY 920

Query: 69  ANQQKTEMKKLVEEMLSSGVIRPSTSPYSSHVLLVRKKDGS 110
           A+ QK EM++LV+EMLSSG+IRPS SPYSS VLLVRKKDGS
Sbjct: 921 AHHQKEEMERLVDEMLSSGIIRPSKSPYSSPVLLVRKKDGS 961

[Close]

BLAST of CSPI07G12230 vs. ExPASy TrEMBL
Match: A0A5A7UVI2 (Ty3/gypsy retrotransposon protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G005060 PE=4 SV=1)

HSP 1 Score: 133.7 bits (335), Expect = 4.8e-28
Identity = 65/101 (64.36%), Postives = 79/101 (78.22%), Query Frame = 0


Query: 9   LEEVESWEEELIVEESVTVILKSFKDVFEWPETFPSGRAIEHHIHLKKGMDLVNVRPYRY 68
           LEE ++ E+  ++ E +  +LK F  VFEWP T P  R+IEHHI+LK G D VNVRPYRY
Sbjct: 626 LEEEQNQEQGKVIAEPIATLLKQFARVFEWPATLPPQRSIEHHIYLKSGTDPVNVRPYRY 685

Query: 69  ANQQKTEMKKLVEEMLSSGVIRPSTSPYSSHVLLVRKKDGS 110
           A+ QK EM++LV+EMLSSG+IRPS SPYSS VLLVRKKDGS
Sbjct: 686 AHHQKEEMERLVDEMLSSGIIRPSKSPYSSPVLLVRKKDGS 726

[Close]

BLAST of CSPI07G12230 vs. ExPASy TrEMBL
Match: A0A5A7TWF5 (Ty3/gypsy retrotransposon protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold908G00310 PE=4 SV=1)

HSP 1 Score: 131.3 bits (329), Expect = 2.4e-27
Identity = 68/103 (66.02%), Postives = 80/103 (77.67%), Query Frame = 0


Query: 8   SLEEVESWEEELIVE-ESVTVILKSFKDVFEWPETFPSGRAIEHHIHLKKGMDLVNVRPY 67
           SLEE E  +E+   + E +  +LK F  VFEWP T P  R+IEHHI+LK G D VNVRPY
Sbjct: 628 SLEEDEQDQEQGKDDAEPIATLLKQFARVFEWPATLPPQRSIEHHIYLKSGADPVNVRPY 687

Query: 68  RYANQQKTEMKKLVEEMLSSGVIRPSTSPYSSHVLLVRKKDGS 110
           RYA+ QK EM++LV+EMLSSG+IRPS SPYSSHVLLVRKKDGS
Sbjct: 688 RYAHHQKEEMERLVDEMLSSGIIRPSKSPYSSHVLLVRKKDGS 730

[Close]

BLAST of CSPI07G12230 vs. ExPASy TrEMBL
Match: A0A5A7UY55 (Ty3/gypsy retrotransposon protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold518G00850 PE=4 SV=1)

HSP 1 Score: 130.2 bits (326), Expect = 5.3e-27
Identity = 61/88 (69.32%), Postives = 72/88 (81.82%), Query Frame = 0


Query: 22  EESVTVILKSFKDVFEWPETFPSGRAIEHHIHLKKGMDLVNVRPYRYANQQKTEMKKLVE 81
           EE++  +LK F  VFEWP   P  R+I+HHI+LK GMD VNVRPYRYA+ QK EM++LV+
Sbjct: 247 EEAIATLLKQFASVFEWPTALPPQRSIDHHIYLKSGMDPVNVRPYRYAHHQKEEMERLVD 306

Query: 82  EMLSSGVIRPSTSPYSSHVLLVRKKDGS 110
           EMLSSG+IRPS SPYSS VLLVRKKDGS
Sbjct: 307 EMLSSGIIRPSKSPYSSPVLLVRKKDGS 334

[Close]

BLAST of CSPI07G12230 vs. ExPASy TrEMBL
Match: A0A5D3CWK0 (Ty3/gypsy retrotransposon protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1081G00050 PE=4 SV=1)

HSP 1 Score: 130.2 bits (326), Expect = 5.3e-27
Identity = 61/88 (69.32%), Postives = 72/88 (81.82%), Query Frame = 0


Query: 22  EESVTVILKSFKDVFEWPETFPSGRAIEHHIHLKKGMDLVNVRPYRYANQQKTEMKKLVE 81
           EE++  +LK F  VFEWP   P  R+I+HHI+LK GMD VNVRPYRYA+ QK EM++LV+
Sbjct: 283 EEAIATLLKQFASVFEWPTALPPQRSIDHHIYLKSGMDPVNVRPYRYAHHQKEEMERLVD 342

Query: 82  EMLSSGVIRPSTSPYSSHVLLVRKKDGS 110
           EMLSSG+IRPS SPYSS VLLVRKKDGS
Sbjct: 343 EMLSSGIIRPSKSPYSSPVLLVRKKDGS 370

[Close]

BLAST of CSPI07G12230 vs. NCBI nr
Match: XP_031745972.1 (uncharacterized protein LOC116406393 [Cucumis sativus])

HSP 1 Score: 138.3 bits (347), Expect = 4.0e-29
Identity = 72/108 (66.67%), Postives = 83/108 (76.85%), Query Frame = 0


Query: 2   ALERSESLEEVESWEEELIVEESVTVILKSFKDVFEWPETFPSGRAIEHHIHLKKGMDLV 61
           A+E      E    EE L V+E+V+ +LK F+DVF WPET P  R+IEHHI+LK+G D V
Sbjct: 567 AMETMYEPPEDNGIEEVLAVDEAVSDVLKKFEDVFTWPETLPPRRSIEHHIYLKQGTDPV 626

Query: 62  NVRPYRYANQQKTEMKKLVEEMLSSGVIRPSTSPYSSHVLLVRKKDGS 110
           NVRPYRY  QQK EM++LVEEMLSSGVIRPS SPYSS VLLVRKKDGS
Sbjct: 627 NVRPYRYGYQQKAEMERLVEEMLSSGVIRPSNSPYSSPVLLVRKKDGS 674

[Close]

BLAST of CSPI07G12230 vs. NCBI nr
Match: KAA0058186.1 (Ty3/gypsy retrotransposon protein [Cucumis melo var. makuwa])

HSP 1 Score: 133.7 bits (335), Expect = 9.9e-28
Identity = 65/101 (64.36%), Postives = 79/101 (78.22%), Query Frame = 0


Query: 9   LEEVESWEEELIVEESVTVILKSFKDVFEWPETFPSGRAIEHHIHLKKGMDLVNVRPYRY 68
           LEE ++ E+  ++ E +  +LK F  VFEWP T P  R+IEHHI+LK G D VNVRPYRY
Sbjct: 626 LEEEQNQEQGKVIAEPIATLLKQFARVFEWPATLPPQRSIEHHIYLKSGTDPVNVRPYRY 685

Query: 69  ANQQKTEMKKLVEEMLSSGVIRPSTSPYSSHVLLVRKKDGS 110
           A+ QK EM++LV+EMLSSG+IRPS SPYSS VLLVRKKDGS
Sbjct: 686 AHHQKEEMERLVDEMLSSGIIRPSKSPYSSPVLLVRKKDGS 726

[Close]

BLAST of CSPI07G12230 vs. NCBI nr
Match: KAA0044648.1 (Ty3/gypsy retrotransposon protein [Cucumis melo var. makuwa])

HSP 1 Score: 133.7 bits (335), Expect = 9.9e-28
Identity = 65/101 (64.36%), Postives = 79/101 (78.22%), Query Frame = 0


Query: 9   LEEVESWEEELIVEESVTVILKSFKDVFEWPETFPSGRAIEHHIHLKKGMDLVNVRPYRY 68
           LEE ++ E+  ++ E +  +LK F  VFEWP T P  R+IEHHI+LK G D VNVRPYRY
Sbjct: 861 LEEEQNQEQGKVIAEPIATLLKQFARVFEWPATLPPQRSIEHHIYLKSGTDPVNVRPYRY 920

Query: 69  ANQQKTEMKKLVEEMLSSGVIRPSTSPYSSHVLLVRKKDGS 110
           A+ QK EM++LV+EMLSSG+IRPS SPYSS VLLVRKKDGS
Sbjct: 921 AHHQKEEMERLVDEMLSSGIIRPSKSPYSSPVLLVRKKDGS 961

[Close]

BLAST of CSPI07G12230 vs. NCBI nr
Match: KAA0047238.1 (Ty3/gypsy retrotransposon protein [Cucumis melo var. makuwa])

HSP 1 Score: 131.3 bits (329), Expect = 4.9e-27
Identity = 68/103 (66.02%), Postives = 80/103 (77.67%), Query Frame = 0


Query: 8   SLEEVESWEEELIVE-ESVTVILKSFKDVFEWPETFPSGRAIEHHIHLKKGMDLVNVRPY 67
           SLEE E  +E+   + E +  +LK F  VFEWP T P  R+IEHHI+LK G D VNVRPY
Sbjct: 628 SLEEDEQDQEQGKDDAEPIATLLKQFARVFEWPATLPPQRSIEHHIYLKSGADPVNVRPY 687

Query: 68  RYANQQKTEMKKLVEEMLSSGVIRPSTSPYSSHVLLVRKKDGS 110
           RYA+ QK EM++LV+EMLSSG+IRPS SPYSSHVLLVRKKDGS
Sbjct: 688 RYAHHQKEEMERLVDEMLSSGIIRPSKSPYSSHVLLVRKKDGS 730

[Close]

BLAST of CSPI07G12230 vs. NCBI nr
Match: KAA0059567.1 (Ty3/gypsy retrotransposon protein [Cucumis melo var. makuwa])

HSP 1 Score: 130.2 bits (326), Expect = 1.1e-26
Identity = 61/88 (69.32%), Postives = 72/88 (81.82%), Query Frame = 0


Query: 22  EESVTVILKSFKDVFEWPETFPSGRAIEHHIHLKKGMDLVNVRPYRYANQQKTEMKKLVE 81
           EE++  +LK F  VFEWP   P  R+I+HHI+LK GMD VNVRPYRYA+ QK EM++LV+
Sbjct: 247 EEAIATLLKQFASVFEWPTALPPQRSIDHHIYLKSGMDPVNVRPYRYAHHQKEEMERLVD 306

Query: 82  EMLSSGVIRPSTSPYSSHVLLVRKKDGS 110
           EMLSSG+IRPS SPYSS VLLVRKKDGS
Sbjct: 307 EMLSSGIIRPSKSPYSSPVLLVRKKDGS 334

[Close]

BLAST of CSPI07G12230 vs. TAIR 10
Match: ATMG00850.1 (DNA/RNA polymerases superfamily protein )

HSP 1 Score: 45.4 bits (106), Expect = 3.3e-05
Identity = 21/37 (56.76%), Postives = 29/37 (78.38%), Query Frame = 0


Query: 72  QKTEMKKLVEEMLSSGVIRPSTSPYSSHVLLVRKKDG 109
           ++T +K  + EML + +I+PS SPYSS VLLV+KKDG
Sbjct: 41  RRTRLKNWLGEMLEARIIQPSISPYSSPVLLVQKKDG 77

The following BLAST results are available for this feature:

BLAST of CSPI07G12230 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q99315	2.2e-06	40.32	Transposon Ty3-G Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 20...	[more]
Q7LHG5	2.2e-06	40.32	Transposon Ty3-I Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 20...	[more]
Q8I7P9	9.4e-05	30.68	Retrovirus-related Pol polyprotein from transposon opus OS=Drosophila melanogast...	[more]
P92522	4.7e-04	56.76	Uncharacterized mitochondrial protein AtMg00850 OS=Arabidopsis thaliana OX=3702 ...	[more]

BLAST of CSPI07G12230 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7TP42	4.8e-28	64.36	Ty3/gypsy retrotransposon protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C2...	[more]
A0A5A7UVI2	4.8e-28	64.36	Ty3/gypsy retrotransposon protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C2...	[more]
A0A5A7TWF5	2.4e-27	66.02	Ty3/gypsy retrotransposon protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C2...	[more]
A0A5A7UY55	5.3e-27	69.32	Ty3/gypsy retrotransposon protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C2...	[more]
A0A5D3CWK0	5.3e-27	69.32	Ty3/gypsy retrotransposon protein OS=Cucumis melo var. makuwa OX=1194695 GN=E567...	[more]

BLAST of CSPI07G12230 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_031745972.1	4.0e-29	66.67	uncharacterized protein LOC116406393 [Cucumis sativus]	[more]
KAA0058186.1	9.9e-28	64.36	Ty3/gypsy retrotransposon protein [Cucumis melo var. makuwa]	[more]
KAA0044648.1	9.9e-28	64.36	Ty3/gypsy retrotransposon protein [Cucumis melo var. makuwa]	[more]
KAA0047238.1	4.9e-27	66.02	Ty3/gypsy retrotransposon protein [Cucumis melo var. makuwa]	[more]
KAA0059567.1	1.1e-26	69.32	Ty3/gypsy retrotransposon protein [Cucumis melo var. makuwa]	[more]

BLAST of CSPI07G12230 vs. TAIR 10


Match Name	E-value	Identity	Description
ATMG00850.1	3.3e-05	56.76	DNA/RNA polymerases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.10.10.10	HIV Type 1 Reverse Transcriptase, subunit A, domain 1	coord: 43..109 e-value: 9.1E-19 score: 69.5
None	No IPR available	PANTHER	PTHR24559	TRANSPOSON TY3-I GAG-POL POLYPROTEIN	coord: 23..109
None	No IPR available	PANTHER	PTHR24559:SF319	SUBFAMILY NOT NAMED	coord: 23..109
IPR043502	DNA/RNA polymerase superfamily	SUPERFAMILY	56672	DNA/RNA polymerases	coord: 29..108

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI07G12230.1	CSPI07G12230.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
biological_process	GO:0015074	DNA integration
cellular_component	GO:0016020	membrane
cellular_component	GO:0031967	organelle envelope
molecular_function	GO:0051537	2 iron, 2 sulfur cluster binding
molecular_function	GO:0030246	carbohydrate binding
molecular_function	GO:0047938	glucose-6-phosphate 1-epimerase activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0003676	nucleic acid binding