CSPI07G11950 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI07G11950
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionE3 ubiquitin-protein ligase
LocationChr7: 10182827 .. 10185707 (-)
RNA-Seq ExpressionCSPI07G11950
SyntenyCSPI07G11950
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAAAAAAAAGATTCGGTTGATTATTGATTCTTTCTTCTTCTCTATTTGGTCTTTTTTGTTTTGTTTTTTGGTTTCTTGAAGTTAGGCCAACGCCTTTCGCGTATCCTAAACCGTTTGATTGTTGATTTCATTTGAATTTCTCTGTTTTTTTTTTTTTTTCCATTTTTCTTGAGTTTCTGGTCACCCTCTGCGACCCAACATCAACTTCCTGTCTTCTTTTTTTTCAACCGTTTTTTGTTGCCTTCTTCTTGCTCTTTATGCCTTTATATACGCCTTGATTCCAATTCTTTGTTTGTTATGGGATTCCCTTTTAAGGCTTCTCTCTGAATTGGCAGTTTTGGGCCTGCACGATAGGTGTTTGATGTTTTGCCTCACTGAATACCTTATAATTTGGGACATGAGACTTTCATGAATTTGGATGGTTTGCTTTTGTCCGAAATTGGGTTCCGTTTCTCTTTTAATCATTACGGGCAAAACTTGTGTGATTTTTGTTGTCTTCGTGCTTGGACTTGCCCTTTCCTTTTAGCTATCCTTTCATGAGAACCATCCCCTTTTCTTTCTTCATATTTCCAACTTTCTCCATGTCCGTGTAGTTTGTTGTCTTGTCTTGATAAGGAACCTGGTTGGTTTTAGTTGGAAAAGTTCATTTTGCAATGATTCATTCTGGTTTCTTCCTTTCGGGACCATCATTTAATTCTGATGCTACTACGTCTTTCATATCAATTTCGGTGTCTGAAGAAGATGGCGCTATGGCTGATGATATCAGAAACGAAGCATCTGATTCTGTCCCCCCTTCCTTTTGGGTTAAAATGGCGATGAAAATATCTAGAGCTAGATGGTTTATTTTTTTGAGAAGAGTTTTCCACTACCAAAACGGATCGAGATCTGATCTTGGGCCTAATCCTTTCAATTCGGGTTCGTGGATGGCGATGGAACTTGTAGCTTTGTTGTTTCAGCTAATTATCTCGGCGTTTACCCTTGCCATTTCACAGGCTGAAAAGCCAGTTTGGCCTATGAGGTTGTGGATAGGTGGTTATGATCTCGGCTGTGTTCTTAGCTTACTGCTTCTCTATGGCCGCCATCAGTATCATTATCTAATGCAGAGAGATGGTAACAGTCTTTCAGATATTGAACACGAACAGCAAAGGACCAATGAATCATCAAGGTAATCTTCAACCTACTAGTTTCCTATGAATTTATTTCATAGTTATTTCTTTGAAAAGCTAGTGAGGATTGAATTTAGATTGGACAAAGAAGCTTAGGGTCAATCATATTCTAACATTCTTTTCCCATAACGCTGAGGTTAAAAGTTCATGCAGTTTCTATGAGTCTTTATTTTACTCAAAAACTGAATTGAAGGATGGAAGATTTTCAAATGACCCACAGGGTTAAAAGTCATAATATTGTTTTATTGAAAATAAGTCAATGCAGACTAGGAGATCAATTAAGAGTAAACACAATATTTTATTGAGTTTCTATTTCTTCAATATTCAAAAAGTTTGCATCTGCAAAATTTGACCTCTAATCTGACTACTTTTATTCAACATAATTTCAGGTATTCACATTTGATGAACCGATGCCGCACGTCTCTCGATCTTTTCTTTGCGATATGGTTTGTAATGGGCAATCTTTGGGCCTTCGATTCTCGCTTAGCCTCGTTCCAGAGAGCTCCCAAGCTACATCTACTATGCAGCTTCCTGCTAGTTTGGAATGCCATCTGCTACTCTTTCCCATTTATTCTGTTTCTGCTGCTATGTTGCTGTGTGCCATTGATTAGTAGCCTTACAGGGTATAACATTAACACGGGATCAACTGAAAAAGGAGCATCAGACGATCAAATTTCCCAGCTACCTTGCTGGAGATACAAAGCAGTTGAAGCCAACATCAACCCCAGAAGTCAACTCGACAATAGCAATACAGGACTTCTAAAAGAGGATCCAGTAAGTAATAATTCTCTTCCTGGTTTTGAAAAAACTACAATAGAAAATTCAAATAGAAAGAGCAATGGGGGATTAAAATACTTCCGGTTGCCTTCGGTTTTGCAGGAATGTTGCATTTGCCTTGCCAAGTATATAGACAAGGAAGAAGTGAGACAGTTACCTTGTTCCCATGTGTTTCATCTCAGGTGTGTAGATAAGTGGCTTGCTATTACATCTTCATGCCCTCTCTGTAAGCAACAACTTCAAAGATAGAGAGCCAAGAAATTAGTATAAGCAGTTTAAGTGCTTCATTGGGTTTTTTCTTTTCTTTTCCCCTTCCAAATGGAAATTTTCTGATTTTCACTGTTTTTATGATTTAGTCAGTAGATTAGAGTCCCACATGGTTACTTGAGTGAATTCTAGAGCTCACCAGTTACCTAATTATACTCAAATACTCGAGGTTACATATAGCAGGAAAATGATGCCTTATTTGTAAATATTGTGAGAACTATAGTATTTGCCTAATGAATACAAACCAAGTGTGCTTTTTTCCTCTTTAATCCTGTGAAAGTTATTGTTACCAATAAACTTGCTGAGGCCCCGTCTACATCCTCGACATGATTAGTTGTGTTAAGTTTGTCTGGAAAAATATTGTATGAAAAAGGAAGAAAACTCAAACAATCGGGACTGGTATGCAGGTATGAAGCCTTGCCTACGTGCAGTTTCCTTCTTTGATTAAAGTTTGAGCAATCAACTAGTTGAAACATCAAATGAGAATGTTGTGTGAAAATGATATGTTATCTTGAGTTTAGGCACTCTCTCCTTCTCTCTCACCTTTCCAAACGCAGATTTGTCAACTAAGTTTAACGAGCAATCAACATCTCATTAGAAGAAGGGTTCTTTTTTTAAGATAAGGCATCCAGATCTTAACCACATACCTTGTAAATTGTAATAGCAAAG

mRNA sequence

AAAAAAAAAAAGATTCGGTTGATTATTGATTCTTTCTTCTTCTCTATTTGGTCTTTTTTGTTTTGTTTTTTGGTTTCTTGAAGTTAGGCCAACGCCTTTCGCGTATCCTAAACCGTTTGATTGTTGATTTCATTTGAATTTCTCTGTTTTTTTTTTTTTTTCCATTTTTCTTGAGTTTCTGGTCACCCTCTGCGACCCAACATCAACTTCCTGTCTTCTTTTTTTTCAACCGTTTTTTGTTGCCTTCTTCTTGCTCTTTATGCCTTTATATACGCCTTGATTCCAATTCTTTGTTTGTTATGGGATTCCCTTTTAAGGCTTCTCTCTGAATTGGCAGTTTTGGGCCTGCACGATAGGTGTTTGATGTTTTGCCTCACTGAATACCTTATAATTTGGGACATGAGACTTTCATGAATTTGGATGGTTTGCTTTTGTCCGAAATTGGGTTCCGTTTCTCTTTTAATCATTACGGGCAAAACTTGTGTGATTTTTGTTGTCTTCGTGCTTGGACTTGCCCTTTCCTTTTAGCTATCCTTTCATGAGAACCATCCCCTTTTCTTTCTTCATATTTCCAACTTTCTCCATGTCCGTGTAGTTTGTTGTCTTGTCTTGATAAGGAACCTGGTTGGTTTTAGTTGGAAAAGTTCATTTTGCAATGATTCATTCTGGTTTCTTCCTTTCGGGACCATCATTTAATTCTGATGCTACTACGTCTTTCATATCAATTTCGGTGTCTGAAGAAGATGGCGCTATGGCTGATGATATCAGAAACGAAGCATCTGATTCTGTCCCCCCTTCCTTTTGGGTTAAAATGGCGATGAAAATATCTAGAGCTAGATGGTTTATTTTTTTGAGAAGAGTTTTCCACTACCAAAACGGATCGAGATCTGATCTTGGGCCTAATCCTTTCAATTCGGGTTCGTGGATGGCGATGGAACTTGTAGCTTTGTTGTTTCAGCTAATTATCTCGGCGTTTACCCTTGCCATTTCACAGGCTGAAAAGCCAGTTTGGCCTATGAGGTTGTGGATAGGTGGTTATGATCTCGGCTGTGTTCTTAGCTTACTGCTTCTCTATGGCCGCCATCAGTATCATTATCTAATGCAGAGAGATGGTAACAGTCTTTCAGATATTGAACACGAACAGCAAAGGACCAATGAATCATCAAGGTATTCACATTTGATGAACCGATGCCGCACGTCTCTCGATCTTTTCTTTGCGATATGGTTTGTAATGGGCAATCTTTGGGCCTTCGATTCTCGCTTAGCCTCGTTCCAGAGAGCTCCCAAGCTACATCTACTATGCAGCTTCCTGCTAGTTTGGAATGCCATCTGCTACTCTTTCCCATTTATTCTGTTTCTGCTGCTATGTTGCTGTGTGCCATTGATTAGTAGCCTTACAGGGTATAACATTAACACGGGATCAACTGAAAAAGGAGCATCAGACGATCAAATTTCCCAGCTACCTTGCTGGAGATACAAAGCAGTTGAAGCCAACATCAACCCCAGAAGTCAACTCGACAATAGCAATACAGGACTTCTAAAAGAGGATCCAGAATGTTGCATTTGCCTTGCCAAGTATATAGACAAGGAAGAAGTGAGACAGTTACCTTGTTCCCATGTGTTTCATCTCAGGTATGAAGCCTTGCCTACGTGCAGTTTCCTTCTTTGATTAAAGTTTGAGCAATCAACTAGTTGAAACATCAAATGAGAATGTTGTGTGAAAATGATATGTTATCTTGAGTTTAGGCACTCTCTCCTTCTCTCTCACCTTTCCAAACGCAGATTTGTCAACTAAGTTTAACGAGCAATCAACATCTCATTAGAAGAAGGGTTCTTTTTTTAAGATAAGGCATCCAGATCTTAACCACATACCTTGTAAATTGTAATAGCAAAG

Coding sequence (CDS)

ATGATTCATTCTGGTTTCTTCCTTTCGGGACCATCATTTAATTCTGATGCTACTACGTCTTTCATATCAATTTCGGTGTCTGAAGAAGATGGCGCTATGGCTGATGATATCAGAAACGAAGCATCTGATTCTGTCCCCCCTTCCTTTTGGGTTAAAATGGCGATGAAAATATCTAGAGCTAGATGGTTTATTTTTTTGAGAAGAGTTTTCCACTACCAAAACGGATCGAGATCTGATCTTGGGCCTAATCCTTTCAATTCGGGTTCGTGGATGGCGATGGAACTTGTAGCTTTGTTGTTTCAGCTAATTATCTCGGCGTTTACCCTTGCCATTTCACAGGCTGAAAAGCCAGTTTGGCCTATGAGGTTGTGGATAGGTGGTTATGATCTCGGCTGTGTTCTTAGCTTACTGCTTCTCTATGGCCGCCATCAGTATCATTATCTAATGCAGAGAGATGGTAACAGTCTTTCAGATATTGAACACGAACAGCAAAGGACCAATGAATCATCAAGGTATTCACATTTGATGAACCGATGCCGCACGTCTCTCGATCTTTTCTTTGCGATATGGTTTGTAATGGGCAATCTTTGGGCCTTCGATTCTCGCTTAGCCTCGTTCCAGAGAGCTCCCAAGCTACATCTACTATGCAGCTTCCTGCTAGTTTGGAATGCCATCTGCTACTCTTTCCCATTTATTCTGTTTCTGCTGCTATGTTGCTGTGTGCCATTGATTAGTAGCCTTACAGGGTATAACATTAACACGGGATCAACTGAAAAAGGAGCATCAGACGATCAAATTTCCCAGCTACCTTGCTGGAGATACAAAGCAGTTGAAGCCAACATCAACCCCAGAAGTCAACTCGACAATAGCAATACAGGACTTCTAAAAGAGGATCCAGAATGTTGCATTTGCCTTGCCAAGTATATAGACAAGGAAGAAGTGAGACAGTTACCTTGTTCCCATGTGTTTCATCTCAGGTATGAAGCCTTGCCTACGTGCAGTTTCCTTCTTTGA

Protein sequence

MIHSGFFLSGPSFNSDATTSFISISVSEEDGAMADDIRNEASDSVPPSFWVKMAMKISRARWFIFLRRVFHYQNGSRSDLGPNPFNSGSWMAMELVALLFQLIISAFTLAISQAEKPVWPMRLWIGGYDLGCVLSLLLLYGRHQYHYLMQRDGNSLSDIEHEQQRTNESSRYSHLMNRCRTSLDLFFAIWFVMGNLWAFDSRLASFQRAPKLHLLCSFLLVWNAICYSFPFILFLLLCCCVPLISSLTGYNINTGSTEKGASDDQISQLPCWRYKAVEANINPRSQLDNSNTGLLKEDPECCICLAKYIDKEEVRQLPCSHVFHLRYEALPTCSFLL*
Homology
BLAST of CSPI07G11950 vs. ExPASy Swiss-Prot
Match: Q93Z92 (E3 ubiquitin-protein ligase At4g11680 OS=Arabidopsis thaliana OX=3702 GN=At4g11680 PE=2 SV=1)

HSP 1 Score: 121.3 bits (303), Expect = 2.1e-26
Identity = 75/264 (28.41%), Postives = 127/264 (48.11%), Query Frame = 0

Query: 104 ISAFTLAISQAEKPVWPMRLWIGGYDLGCVLSLLLL----YGRHQYHYLMQRDGNSLSDI 163
           I    L +S+ EKP  P+R+W+ GY + C L +  +      R +  +     G+ L++ 
Sbjct: 123 IGVAVLILSRDEKPNMPLRVWVVGYGIQCWLHMACVCVEYRRRRRRRHPEDGGGSGLTNS 182

Query: 164 EHEQ----QRTNESSRYSHLMNRCRTSLDLFFAIWFVMGNLWAFDSRLASFQRAPKLHLL 223
             +Q     +  +    S+      ++  +F  IW+++G  W           +P+L+ L
Sbjct: 183 SSQQYVSLAQLEDRGETSNPAKHLESANTMFSFIWWIIGFYWVSAGGQTLSSDSPQLYWL 242

Query: 224 CSFLLVWN----AICYSFPFILFLLLCCCVPLISSLTGYNINTGSTEKGASDDQISQLPC 283
           C   L ++      C +   ++ L +CCC+P I ++    +   + ++GAS + I Q+P 
Sbjct: 243 CIIFLGFDVFFVVFCVALACVIGLAVCCCLPCIIAI----LYAVADQEGASKNDIDQMPK 302

Query: 284 WRY-------------KAVEANINPRSQLDNSNTGLLKEDPECCICLAKYIDKEEVRQLP 343
           +R+             + +       S ++ S   L  ED ECCICL +Y D  E+R+LP
Sbjct: 303 FRFTKTGNVEKLSGKARGIMTECGTDSPIERS---LSPEDAECCICLCEYEDGVELRELP 362

BLAST of CSPI07G11950 vs. ExPASy Swiss-Prot
Match: Q9LN71 (E3 ubiquitin-protein ligase At1g12760 OS=Arabidopsis thaliana OX=3702 GN=At1g12760 PE=1 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 6.2e-26
Identity = 81/305 (26.56%), Postives = 142/305 (46.56%), Query Frame = 0

Query: 91  MAMELVALLFQLIISAFTLAISQAEKPVWPMRLWIGGYDLGCVLSLLLL---YGRH---- 150
           + +++V  L  + ++   L +S+ E P+ P+R+W+ GY L CVL ++ +   Y R     
Sbjct: 101 VVLDIVWNLAFVSVATAILVMSRKEHPIMPLRVWLLGYALQCVLHMVCVCVEYRRRNRRR 160

Query: 151 ------------------------QYHYLMQRDGNSLSDIEHEQQRTNESSRYSHLMNRC 210
                                   +   L  R  + + D+      T  SS   HL    
Sbjct: 161 TNRTTTTTPPRSRSSSSSSSSSSLEEEALGSRRNSGVQDLSLGHLDTESSSVAKHL---- 220

Query: 211 RTSLDLFFAIWFVMGNLWAFDSRLASFQRAPKLHLL----CSFLLVWNAICYSFPFILFL 270
            ++  +F  IW+++G  W         Q +P+++ L      F + +   C +   ++ +
Sbjct: 221 ESANTMFSFIWWIIGFYWVSAGGQELAQESPRIYWLSIVFLGFDVFFVVFCVALACVIGI 280

Query: 271 LLCCCVPLISSLTGYNINTGSTEKGASDDQISQLPCWRYKAV-EANINPRSQLDNSNTG- 330
            +CCC+P I ++    +   + ++GAS + I QL  ++++ + +AN +   +   +  G 
Sbjct: 281 AVCCCLPCIIAV----LYAVADQEGASKEDIEQLTKFKFRKLGDANKHTNDEAQGTTEGI 340

Query: 331 -------------LLKEDPECCICLAKYIDKEEVRQLPCSHVFHLRCVDKWLAITSSCPL 346
                        LL+ED ECCICL+ Y D  E+R+LPC H FH  CVDKWL I ++CPL
Sbjct: 341 MTECGTDSPIEHTLLQEDAECCICLSAYEDGTELRELPCGHHFHCSCVDKWLYINATCPL 397

BLAST of CSPI07G11950 vs. ExPASy Swiss-Prot
Match: Q8LDB8 (E3 ubiquitin-protein ligase At1g63170 OS=Arabidopsis thaliana OX=3702 GN=At1g63170 PE=2 SV=2)

HSP 1 Score: 119.4 bits (298), Expect = 8.1e-26
Identity = 79/302 (26.16%), Postives = 141/302 (46.69%), Query Frame = 0

Query: 91  MAMELVALLFQLIISAFTLAISQAEKPVWPMRLWIGGYDLGCVLSLLLLYGRHQYHYLMQ 150
           + ++ V  L  ++++   L +S  E P  P+R+WI GY L C++ ++ +   ++     +
Sbjct: 75  VVLDFVWNLAFVVVATAVLVLSSDENPNMPLRVWIIGYGLQCMMHMVCVCVEYRRRNSRR 134

Query: 151 R----------DGNSLSDIEHEQ----------------QRTNESSRYSHLMNRCRTSLD 210
           R            +S S ++ E+                Q  NE++ ++  +    T + 
Sbjct: 135 RRDLSPRSSSSSSSSSSSMDEEEGLGLSRNSDERYLELGQLENENNSFAKHLESANTMIS 194

Query: 211 LFFAIWFVMGNLWAFDSRLASFQRAPKLHLLC----SFLLVWNAICYSFPFILFLLLCCC 270
               IW+V+G  W         Q +P+L+ LC     F + +   C +   ++ + +CCC
Sbjct: 195 F---IWWVIGFYWVSSGGQELAQGSPQLYWLCIVFLGFDVFFVVFCVALACVIGIAVCCC 254

Query: 271 VPLISSLTGYNINTGSTEKGASDDQISQLPCWRYKAVEANI-----NPRSQLDNSNT--- 330
           +P I ++    +   + ++GAS + I QL  ++++ V   +       + Q D+      
Sbjct: 255 LPCIIAV----LYAVAEQEGASKEDIDQLTKFKFRKVGDTMKHTVDEEQGQGDSGGVMTE 314

Query: 331 ---------GLLKEDPECCICLAKYIDKEEVRQLPCSHVFHLRCVDKWLAITSSCPLCKQ 346
                     L  ED ECCICL+ Y D+ E+R+LPC H FH  CVDKWL I ++CPLCK 
Sbjct: 315 CGTDSPVEHALPHEDAECCICLSAYEDETELRELPCGHHFHCGCVDKWLYINATCPLCKY 369

BLAST of CSPI07G11950 vs. ExPASy Swiss-Prot
Match: Q8GUU2 (E3 ubiquitin protein ligase RIE1 OS=Arabidopsis thaliana OX=3702 GN=RIE1 PE=2 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 4.0e-25
Identity = 74/268 (27.61%), Postives = 127/268 (47.39%), Query Frame = 0

Query: 102 LIISAFTLAISQAEKPVWPMRLWIGGYDLGCVLSLLLLYGRHQYHYLMQRDGNSLSDIEH 161
           ++ SA  L  +  E+P  P+R+WI  Y L C+  ++L++  + +     R    L   +H
Sbjct: 89  VLASAVMLVGTVEERPNEPIRVWICVYGLQCLFHVVLVWSEY-WRRNSTRRARDLESYDH 148

Query: 162 E--------QQRTNESSRYSHLMNRCRTSLDLFFAIWFVMGNLWAFDSRLASFQRAPKLH 221
           E        +Q ++++S     + RC +   +   IW+++G  W  +        AP L+
Sbjct: 149 EDYNIEYDYEQDSDDNSTTYSFVKRCESINTVISFIWWIIGFYWVVEGGDKLLGEAPNLY 208

Query: 222 LLCSFLLV----WNAICYSFPFILFLLLCCCVPLISSLTGYNINTGSTEKGASDDQISQL 281
            L    L     +   C     ++ + LCCC+P I +L    +   +  +G S+ ++  L
Sbjct: 209 WLSVIFLAIDVFFAVFCVVLACLVGIALCCCLPCIIAL----LYAVAGTEGVSEAELGVL 268

Query: 282 PCWRYKAVEAN----INPRSQLDNSNTG--------LLKEDPECCICLAKYIDKEEVRQL 341
           P +++KA  +N      P   +     G        LL ED +CCICL+ Y D  E+  L
Sbjct: 269 PLYKFKAFHSNEKNITGPGKMVPIPINGLCLATERTLLAEDADCCICLSSYEDGAELHAL 328

Query: 342 PCSHVFHLRCVDKWLAITSSCPLCKQQL 346
           PC+H FH  C+ KWL + ++CPLCK  +
Sbjct: 329 PCNHHFHSTCIVKWLKMRATCPLCKYNI 351

BLAST of CSPI07G11950 vs. ExPASy Swiss-Prot
Match: Q9ZT49 (Probable E3 ubiquitin-protein ligase ATL45 OS=Arabidopsis thaliana OX=3702 GN=ATL45 PE=1 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 1.9e-11
Identity = 34/88 (38.64%), Postives = 46/88 (52.27%), Query Frame = 0

Query: 259 KGASDDQISQLPCWRYKAVEANINPRSQLDNSNTGLLKEDPECCICLAKYIDKEEVRQLP 318
           KG     +  LP   Y A  +       L  S+ G      EC IC+ ++ + EE+R LP
Sbjct: 71  KGLKKKALQALPKSTYTASASTAAAADDLPCSSVGDGDSSTECAICITEFSEGEEIRILP 130

Query: 319 -CSHVFHLRCVDKWLAITSSCPLCKQQL 346
            CSH FH+ C+DKWL   SSCP C++ L
Sbjct: 131 LCSHAFHVACIDKWLTSRSSCPSCRRIL 158

BLAST of CSPI07G11950 vs. ExPASy TrEMBL
Match: A0A0A0K4H0 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G278230 PE=4 SV=1)

HSP 1 Score: 714.9 bits (1844), Expect = 1.6e-202
Identity = 346/347 (99.71%), Postives = 347/347 (100.00%), Query Frame = 0

Query: 1   MIHSGFFLSGPSFNSDATTSFISISVSEEDGAMADDIRNEASDSVPPSFWVKMAMKISRA 60
           MIHSGFFLSGPSFNSDATTSFISISVSEEDGAMADDIRNEASDSVPPSFWVKMAMKISRA
Sbjct: 1   MIHSGFFLSGPSFNSDATTSFISISVSEEDGAMADDIRNEASDSVPPSFWVKMAMKISRA 60

Query: 61  RWFIFLRRVFHYQNGSRSDLGPNPFNSGSWMAMELVALLFQLIISAFTLAISQAEKPVWP 120
           RWFIFLRRVFHYQNGSRSDLGPNPFNSGSWMAMELVALLFQLIISAFTLAISQAEKPVWP
Sbjct: 61  RWFIFLRRVFHYQNGSRSDLGPNPFNSGSWMAMELVALLFQLIISAFTLAISQAEKPVWP 120

Query: 121 MRLWIGGYDLGCVLSLLLLYGRHQYHYLMQRDGNSLSDIEHEQQRTNESSRYSHLMNRCR 180
           MRLWIGGYDLGCVLSLLLLYGRH+YHYLMQRDGNSLSDIEHEQQRTNESSRYSHLMNRCR
Sbjct: 121 MRLWIGGYDLGCVLSLLLLYGRHRYHYLMQRDGNSLSDIEHEQQRTNESSRYSHLMNRCR 180

Query: 181 TSLDLFFAIWFVMGNLWAFDSRLASFQRAPKLHLLCSFLLVWNAICYSFPFILFLLLCCC 240
           TSLDLFFAIWFVMGNLWAFDSRLASFQRAPKLHLLCSFLLVWNAICYSFPFILFLLLCCC
Sbjct: 181 TSLDLFFAIWFVMGNLWAFDSRLASFQRAPKLHLLCSFLLVWNAICYSFPFILFLLLCCC 240

Query: 241 VPLISSLTGYNINTGSTEKGASDDQISQLPCWRYKAVEANINPRSQLDNSNTGLLKEDPE 300
           VPLISSLTGYNINTGSTEKGASDDQISQLPCWRYKAVEANINPRSQLDNSNTGLLKEDPE
Sbjct: 241 VPLISSLTGYNINTGSTEKGASDDQISQLPCWRYKAVEANINPRSQLDNSNTGLLKEDPE 300

Query: 301 CCICLAKYIDKEEVRQLPCSHVFHLRCVDKWLAITSSCPLCKQQLQR 348
           CCICLAKYIDKEEVRQLPCSHVFHLRCVDKWLAITSSCPLCKQQLQR
Sbjct: 301 CCICLAKYIDKEEVRQLPCSHVFHLRCVDKWLAITSSCPLCKQQLQR 347

BLAST of CSPI07G11950 vs. ExPASy TrEMBL
Match: A0A1S3CI94 (E3 ubiquitin-protein ligase At4g11680 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103501269 PE=4 SV=1)

HSP 1 Score: 688.3 bits (1775), Expect = 1.6e-194
Identity = 333/347 (95.97%), Postives = 338/347 (97.41%), Query Frame = 0

Query: 1   MIHSGFFLSGPSFNSDATTSFISISVSEEDGAMADDIRNEASDSVPPSFWVKMAMKISRA 60
           MIHSGFFLSGPSFNSDATTSFISISVS EDGAMADDIRNEASDSVPPSFWVKMAMKISRA
Sbjct: 1   MIHSGFFLSGPSFNSDATTSFISISVSGEDGAMADDIRNEASDSVPPSFWVKMAMKISRA 60

Query: 61  RWFIFLRRVFHYQNGSRSDLGPNPFNSGSWMAMELVALLFQLIISAFTLAISQAEKPVWP 120
           RWFIFLRRVFHYQNGSRSDLGPNPFNSG WMAMELVALLFQLIIS FTLAISQAEKPVWP
Sbjct: 61  RWFIFLRRVFHYQNGSRSDLGPNPFNSGLWMAMELVALLFQLIISVFTLAISQAEKPVWP 120

Query: 121 MRLWIGGYDLGCVLSLLLLYGRHQYHYLMQRDGNSLSDIEHEQQRTNESSRYSHLMNRCR 180
           MRLWIGGYDLGCVLSLLLLYGRH+YHYLMQ + N LSDIEHEQQR+NESSRYSHLMNRCR
Sbjct: 121 MRLWIGGYDLGCVLSLLLLYGRHRYHYLMQGNDNRLSDIEHEQQRSNESSRYSHLMNRCR 180

Query: 181 TSLDLFFAIWFVMGNLWAFDSRLASFQRAPKLHLLCSFLLVWNAICYSFPFILFLLLCCC 240
           TSLDLFFAIWFVMGNLW FDSRLASFQRAPKLHLLCSFLLVWNAICYSFPFILFLLLCCC
Sbjct: 181 TSLDLFFAIWFVMGNLWVFDSRLASFQRAPKLHLLCSFLLVWNAICYSFPFILFLLLCCC 240

Query: 241 VPLISSLTGYNINTGSTEKGASDDQISQLPCWRYKAVEANINPRSQLDNSNTGLLKEDPE 300
           VPLISSLTGYNIN GS+EKGASDDQISQLPCWRYKAVEANINPRSQ DNSNTGLLKEDPE
Sbjct: 241 VPLISSLTGYNINMGSSEKGASDDQISQLPCWRYKAVEANINPRSQPDNSNTGLLKEDPE 300

Query: 301 CCICLAKYIDKEEVRQLPCSHVFHLRCVDKWLAITSSCPLCKQQLQR 348
           CCICLAKYIDKEEVRQLPCSHVFHL+CVDKWLAITSSCPLCKQQLQR
Sbjct: 301 CCICLAKYIDKEEVRQLPCSHVFHLKCVDKWLAITSSCPLCKQQLQR 347

BLAST of CSPI07G11950 vs. ExPASy TrEMBL
Match: A0A5D3CCB0 (E3 ubiquitin-protein ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold265G001490 PE=4 SV=1)

HSP 1 Score: 667.2 bits (1720), Expect = 3.9e-188
Identity = 332/382 (86.91%), Postives = 337/382 (88.22%), Query Frame = 0

Query: 1   MIHSGFFLSGPSFNSDATTSFISISVSEEDGAMADDIRNEASDSVPPSFWVKMAMKISRA 60
           MIHSGFFLSGPSFNSDATTSFISISVS EDGAMADDIRNEASDSVPPSFWVKMAMKISRA
Sbjct: 1   MIHSGFFLSGPSFNSDATTSFISISVSGEDGAMADDIRNEASDSVPPSFWVKMAMKISRA 60

Query: 61  RWFIFLRRVFHYQNGSRSDLGPNPFNSGSWMAMELVALLFQLIISAFTLAISQAEKPVWP 120
           RWFIFLRRVFHYQNGSRSDLGPNPFNSG WMAMELVALLFQLIIS FTLAISQAEKPVWP
Sbjct: 61  RWFIFLRRVFHYQNGSRSDLGPNPFNSGLWMAMELVALLFQLIISVFTLAISQAEKPVWP 120

Query: 121 MRLWIGGYDLGCVLSLLLLYGRHQYHYLMQRDGNSLSDIEHEQQRTNESSRYSHLMNRCR 180
           MRLWIGGYDLGCVLSLLLLYGRH+YHYLMQ + N LS IEHEQQR+NESSRYSHLMNRCR
Sbjct: 121 MRLWIGGYDLGCVLSLLLLYGRHRYHYLMQGNDNRLSYIEHEQQRSNESSRYSHLMNRCR 180

Query: 181 TSLDLFFAIWFVMGNLWAFDSRLASFQRAPKLHLLCSFLLVWNAICYSFPFILFLLLCCC 240
           TSLDLFFAIWFVMGNLW FDSRLASFQRAPKLHLLCSFLLVWNAICYSFPFILFLLLCCC
Sbjct: 181 TSLDLFFAIWFVMGNLWVFDSRLASFQRAPKLHLLCSFLLVWNAICYSFPFILFLLLCCC 240

Query: 241 VPLISSLTGYNINTGSTEKGASDDQISQLPCWRYKAVEANINPRSQLDNSNTGLLKEDP- 300
           VPLISSLTGYNIN GS+EKGASDDQISQLPCWRYKAVEANINPRSQ DNSNTGLLKEDP 
Sbjct: 241 VPLISSLTGYNINMGSSEKGASDDQISQLPCWRYKAVEANINPRSQPDNSNTGLLKEDPV 300

Query: 301 ----------------------------------ECCICLAKYIDKEEVRQLPCSHVFHL 348
                                             ECCICLAKYIDKEEVRQLPCSHVFHL
Sbjct: 301 SNNSLPGFEKTTEENSNKKSDGGLKYFRLPPILQECCICLAKYIDKEEVRQLPCSHVFHL 360

BLAST of CSPI07G11950 vs. ExPASy TrEMBL
Match: A0A1S3CIA1 (E3 ubiquitin-protein ligase At4g11680 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103501269 PE=4 SV=1)

HSP 1 Score: 632.1 bits (1629), Expect = 1.4e-177
Identity = 302/315 (95.87%), Postives = 307/315 (97.46%), Query Frame = 0

Query: 33  MADDIRNEASDSVPPSFWVKMAMKISRARWFIFLRRVFHYQNGSRSDLGPNPFNSGSWMA 92
           MADDIRNEASDSVPPSFWVKMAMKISRARWFIFLRRVFHYQNGSRSDLGPNPFNSG WMA
Sbjct: 1   MADDIRNEASDSVPPSFWVKMAMKISRARWFIFLRRVFHYQNGSRSDLGPNPFNSGLWMA 60

Query: 93  MELVALLFQLIISAFTLAISQAEKPVWPMRLWIGGYDLGCVLSLLLLYGRHQYHYLMQRD 152
           MELVALLFQLIIS FTLAISQAEKPVWPMRLWIGGYDLGCVLSLLLLYGRH+YHYLMQ +
Sbjct: 61  MELVALLFQLIISVFTLAISQAEKPVWPMRLWIGGYDLGCVLSLLLLYGRHRYHYLMQGN 120

Query: 153 GNSLSDIEHEQQRTNESSRYSHLMNRCRTSLDLFFAIWFVMGNLWAFDSRLASFQRAPKL 212
            N LSDIEHEQQR+NESSRYSHLMNRCRTSLDLFFAIWFVMGNLW FDSRLASFQRAPKL
Sbjct: 121 DNRLSDIEHEQQRSNESSRYSHLMNRCRTSLDLFFAIWFVMGNLWVFDSRLASFQRAPKL 180

Query: 213 HLLCSFLLVWNAICYSFPFILFLLLCCCVPLISSLTGYNINTGSTEKGASDDQISQLPCW 272
           HLLCSFLLVWNAICYSFPFILFLLLCCCVPLISSLTGYNIN GS+EKGASDDQISQLPCW
Sbjct: 181 HLLCSFLLVWNAICYSFPFILFLLLCCCVPLISSLTGYNINMGSSEKGASDDQISQLPCW 240

Query: 273 RYKAVEANINPRSQLDNSNTGLLKEDPECCICLAKYIDKEEVRQLPCSHVFHLRCVDKWL 332
           RYKAVEANINPRSQ DNSNTGLLKEDPECCICLAKYIDKEEVRQLPCSHVFHL+CVDKWL
Sbjct: 241 RYKAVEANINPRSQPDNSNTGLLKEDPECCICLAKYIDKEEVRQLPCSHVFHLKCVDKWL 300

Query: 333 AITSSCPLCKQQLQR 348
           AITSSCPLCKQQLQR
Sbjct: 301 AITSSCPLCKQQLQR 315

BLAST of CSPI07G11950 vs. ExPASy TrEMBL
Match: A0A6J1CCU0 (E3 ubiquitin-protein ligase At1g63170 OS=Momordica charantia OX=3673 GN=LOC111010402 PE=4 SV=1)

HSP 1 Score: 616.7 bits (1589), Expect = 6.0e-173
Identity = 301/347 (86.74%), Postives = 318/347 (91.64%), Query Frame = 0

Query: 1   MIHSGFFLSGPSFNSDATTSFISISVSEEDGAMADDIRNEASDSVPPSFWVKMAMKISRA 60
           MIHSGFFLSGPSFNSDATTSFISISVSE+DGA ADDIRNE SDS+PPSFWV+ AMK SRA
Sbjct: 1   MIHSGFFLSGPSFNSDATTSFISISVSEDDGATADDIRNETSDSLPPSFWVRTAMKASRA 60

Query: 61  RWFIFLRRVFHYQNGSRSDLGPNPFNSGSWMAMELVALLFQLIISAFTLAISQAEKPVWP 120
           RWFIFLRRVFHYQNGSRSDLGPNPFNS +WMAMELVALLFQLIIS FTLAISQ E+PVWP
Sbjct: 61  RWFIFLRRVFHYQNGSRSDLGPNPFNSSAWMAMELVALLFQLIISVFTLAISQTERPVWP 120

Query: 121 MRLWIGGYDLGCVLSLLLLYGRHQYHYLMQRDGNSLSDIEHEQQRTNESSRYSHLMNRCR 180
           MRLWIGGYDLGC+LSLLLLYGR++Y YLMQ DGNSL DI  EQQR+NESSRYSHL N+CR
Sbjct: 121 MRLWIGGYDLGCLLSLLLLYGRYRYLYLMQGDGNSLLDI--EQQRSNESSRYSHLTNKCR 180

Query: 181 TSLDLFFAIWFVMGNLWAFDSRLASFQRAPKLHLLCSFLLVWNAICYSFPFILFLLLCCC 240
           T L+LFFAIWFVMGNLW FDSR  SFQRAPKLHLLCS LLVWNAICYSFPFILFLLLCCC
Sbjct: 181 TLLELFFAIWFVMGNLWVFDSRFGSFQRAPKLHLLCSILLVWNAICYSFPFILFLLLCCC 240

Query: 241 VPLISSLTGYNINTGSTEKGASDDQISQLPCWRYKAVEANINPRSQLDNSNTGLLKEDPE 300
           VPLISSL GYN+N GSTEKGASDDQISQLP WRYKAV++NI P +Q D S+TG  KEDPE
Sbjct: 241 VPLISSLIGYNMNMGSTEKGASDDQISQLPSWRYKAVDSNIKPGNQPD-SHTGPPKEDPE 300

Query: 301 CCICLAKYIDKEEVRQLPCSHVFHLRCVDKWLAITSSCPLCKQQLQR 348
           CCICLAKY DKEEVRQLPCSHVFH +CVDKWLAITSSCPLCKQQLQR
Sbjct: 301 CCICLAKYKDKEEVRQLPCSHVFHQKCVDKWLAITSSCPLCKQQLQR 344

BLAST of CSPI07G11950 vs. NCBI nr
Match: XP_011659087.1 (E3 ubiquitin-protein ligase At4g11680 isoform X1 [Cucumis sativus])

HSP 1 Score: 714.9 bits (1844), Expect = 3.3e-202
Identity = 346/347 (99.71%), Postives = 347/347 (100.00%), Query Frame = 0

Query: 1   MIHSGFFLSGPSFNSDATTSFISISVSEEDGAMADDIRNEASDSVPPSFWVKMAMKISRA 60
           MIHSGFFLSGPSFNSDATTSFISISVSEEDGAMADDIRNEASDSVPPSFWVKMAMKISRA
Sbjct: 1   MIHSGFFLSGPSFNSDATTSFISISVSEEDGAMADDIRNEASDSVPPSFWVKMAMKISRA 60

Query: 61  RWFIFLRRVFHYQNGSRSDLGPNPFNSGSWMAMELVALLFQLIISAFTLAISQAEKPVWP 120
           RWFIFLRRVFHYQNGSRSDLGPNPFNSGSWMAMELVALLFQLIISAFTLAISQAEKPVWP
Sbjct: 61  RWFIFLRRVFHYQNGSRSDLGPNPFNSGSWMAMELVALLFQLIISAFTLAISQAEKPVWP 120

Query: 121 MRLWIGGYDLGCVLSLLLLYGRHQYHYLMQRDGNSLSDIEHEQQRTNESSRYSHLMNRCR 180
           MRLWIGGYDLGCVLSLLLLYGRH+YHYLMQRDGNSLSDIEHEQQRTNESSRYSHLMNRCR
Sbjct: 121 MRLWIGGYDLGCVLSLLLLYGRHRYHYLMQRDGNSLSDIEHEQQRTNESSRYSHLMNRCR 180

Query: 181 TSLDLFFAIWFVMGNLWAFDSRLASFQRAPKLHLLCSFLLVWNAICYSFPFILFLLLCCC 240
           TSLDLFFAIWFVMGNLWAFDSRLASFQRAPKLHLLCSFLLVWNAICYSFPFILFLLLCCC
Sbjct: 181 TSLDLFFAIWFVMGNLWAFDSRLASFQRAPKLHLLCSFLLVWNAICYSFPFILFLLLCCC 240

Query: 241 VPLISSLTGYNINTGSTEKGASDDQISQLPCWRYKAVEANINPRSQLDNSNTGLLKEDPE 300
           VPLISSLTGYNINTGSTEKGASDDQISQLPCWRYKAVEANINPRSQLDNSNTGLLKEDPE
Sbjct: 241 VPLISSLTGYNINTGSTEKGASDDQISQLPCWRYKAVEANINPRSQLDNSNTGLLKEDPE 300

Query: 301 CCICLAKYIDKEEVRQLPCSHVFHLRCVDKWLAITSSCPLCKQQLQR 348
           CCICLAKYIDKEEVRQLPCSHVFHLRCVDKWLAITSSCPLCKQQLQR
Sbjct: 301 CCICLAKYIDKEEVRQLPCSHVFHLRCVDKWLAITSSCPLCKQQLQR 347

BLAST of CSPI07G11950 vs. NCBI nr
Match: XP_008463025.1 (PREDICTED: E3 ubiquitin-protein ligase At4g11680 isoform X1 [Cucumis melo])

HSP 1 Score: 688.3 bits (1775), Expect = 3.3e-194
Identity = 333/347 (95.97%), Postives = 338/347 (97.41%), Query Frame = 0

Query: 1   MIHSGFFLSGPSFNSDATTSFISISVSEEDGAMADDIRNEASDSVPPSFWVKMAMKISRA 60
           MIHSGFFLSGPSFNSDATTSFISISVS EDGAMADDIRNEASDSVPPSFWVKMAMKISRA
Sbjct: 1   MIHSGFFLSGPSFNSDATTSFISISVSGEDGAMADDIRNEASDSVPPSFWVKMAMKISRA 60

Query: 61  RWFIFLRRVFHYQNGSRSDLGPNPFNSGSWMAMELVALLFQLIISAFTLAISQAEKPVWP 120
           RWFIFLRRVFHYQNGSRSDLGPNPFNSG WMAMELVALLFQLIIS FTLAISQAEKPVWP
Sbjct: 61  RWFIFLRRVFHYQNGSRSDLGPNPFNSGLWMAMELVALLFQLIISVFTLAISQAEKPVWP 120

Query: 121 MRLWIGGYDLGCVLSLLLLYGRHQYHYLMQRDGNSLSDIEHEQQRTNESSRYSHLMNRCR 180
           MRLWIGGYDLGCVLSLLLLYGRH+YHYLMQ + N LSDIEHEQQR+NESSRYSHLMNRCR
Sbjct: 121 MRLWIGGYDLGCVLSLLLLYGRHRYHYLMQGNDNRLSDIEHEQQRSNESSRYSHLMNRCR 180

Query: 181 TSLDLFFAIWFVMGNLWAFDSRLASFQRAPKLHLLCSFLLVWNAICYSFPFILFLLLCCC 240
           TSLDLFFAIWFVMGNLW FDSRLASFQRAPKLHLLCSFLLVWNAICYSFPFILFLLLCCC
Sbjct: 181 TSLDLFFAIWFVMGNLWVFDSRLASFQRAPKLHLLCSFLLVWNAICYSFPFILFLLLCCC 240

Query: 241 VPLISSLTGYNINTGSTEKGASDDQISQLPCWRYKAVEANINPRSQLDNSNTGLLKEDPE 300
           VPLISSLTGYNIN GS+EKGASDDQISQLPCWRYKAVEANINPRSQ DNSNTGLLKEDPE
Sbjct: 241 VPLISSLTGYNINMGSSEKGASDDQISQLPCWRYKAVEANINPRSQPDNSNTGLLKEDPE 300

Query: 301 CCICLAKYIDKEEVRQLPCSHVFHLRCVDKWLAITSSCPLCKQQLQR 348
           CCICLAKYIDKEEVRQLPCSHVFHL+CVDKWLAITSSCPLCKQQLQR
Sbjct: 301 CCICLAKYIDKEEVRQLPCSHVFHLKCVDKWLAITSSCPLCKQQLQR 347

BLAST of CSPI07G11950 vs. NCBI nr
Match: KAA0048251.1 (E3 ubiquitin-protein ligase [Cucumis melo var. makuwa] >TYK08006.1 E3 ubiquitin-protein ligase [Cucumis melo var. makuwa])

HSP 1 Score: 667.2 bits (1720), Expect = 8.0e-188
Identity = 332/382 (86.91%), Postives = 337/382 (88.22%), Query Frame = 0

Query: 1   MIHSGFFLSGPSFNSDATTSFISISVSEEDGAMADDIRNEASDSVPPSFWVKMAMKISRA 60
           MIHSGFFLSGPSFNSDATTSFISISVS EDGAMADDIRNEASDSVPPSFWVKMAMKISRA
Sbjct: 1   MIHSGFFLSGPSFNSDATTSFISISVSGEDGAMADDIRNEASDSVPPSFWVKMAMKISRA 60

Query: 61  RWFIFLRRVFHYQNGSRSDLGPNPFNSGSWMAMELVALLFQLIISAFTLAISQAEKPVWP 120
           RWFIFLRRVFHYQNGSRSDLGPNPFNSG WMAMELVALLFQLIIS FTLAISQAEKPVWP
Sbjct: 61  RWFIFLRRVFHYQNGSRSDLGPNPFNSGLWMAMELVALLFQLIISVFTLAISQAEKPVWP 120

Query: 121 MRLWIGGYDLGCVLSLLLLYGRHQYHYLMQRDGNSLSDIEHEQQRTNESSRYSHLMNRCR 180
           MRLWIGGYDLGCVLSLLLLYGRH+YHYLMQ + N LS IEHEQQR+NESSRYSHLMNRCR
Sbjct: 121 MRLWIGGYDLGCVLSLLLLYGRHRYHYLMQGNDNRLSYIEHEQQRSNESSRYSHLMNRCR 180

Query: 181 TSLDLFFAIWFVMGNLWAFDSRLASFQRAPKLHLLCSFLLVWNAICYSFPFILFLLLCCC 240
           TSLDLFFAIWFVMGNLW FDSRLASFQRAPKLHLLCSFLLVWNAICYSFPFILFLLLCCC
Sbjct: 181 TSLDLFFAIWFVMGNLWVFDSRLASFQRAPKLHLLCSFLLVWNAICYSFPFILFLLLCCC 240

Query: 241 VPLISSLTGYNINTGSTEKGASDDQISQLPCWRYKAVEANINPRSQLDNSNTGLLKEDP- 300
           VPLISSLTGYNIN GS+EKGASDDQISQLPCWRYKAVEANINPRSQ DNSNTGLLKEDP 
Sbjct: 241 VPLISSLTGYNINMGSSEKGASDDQISQLPCWRYKAVEANINPRSQPDNSNTGLLKEDPV 300

Query: 301 ----------------------------------ECCICLAKYIDKEEVRQLPCSHVFHL 348
                                             ECCICLAKYIDKEEVRQLPCSHVFHL
Sbjct: 301 SNNSLPGFEKTTEENSNKKSDGGLKYFRLPPILQECCICLAKYIDKEEVRQLPCSHVFHL 360

BLAST of CSPI07G11950 vs. NCBI nr
Match: XP_038899145.1 (E3 ubiquitin-protein ligase At4g11680-like [Benincasa hispida])

HSP 1 Score: 662.9 bits (1709), Expect = 1.5e-186
Identity = 318/346 (91.91%), Postives = 327/346 (94.51%), Query Frame = 0

Query: 1   MIHSGFFLSGPSFNSDATTSFISISVSEEDGAMADDIRNEASDSVPPSFWVKMAMKISRA 60
           MIHSGFFLSGPSFNSD TTSFISI VS EDGA AD+I N  SDS+PPSFWV+MAMKISRA
Sbjct: 1   MIHSGFFLSGPSFNSDVTTSFISIPVSGEDGARADEISNHTSDSIPPSFWVRMAMKISRA 60

Query: 61  RWFIFLRRVFHYQNGSRSDLGPNPFNSGSWMAMELVALLFQLIISAFTLAISQAEKPVWP 120
           RWFIFLRR+FHYQNGSRSDLGPNPFNSG WMAMELVALLFQLIISAFTLAISQAE+PVWP
Sbjct: 61  RWFIFLRRIFHYQNGSRSDLGPNPFNSGLWMAMELVALLFQLIISAFTLAISQAERPVWP 120

Query: 121 MRLWIGGYDLGCVLSLLLLYGRHQYHYLMQRDGNSLSDIEHEQQRTNESSRYSHLMNRCR 180
           MRLWIGGYDLGCVLSLLLLYGRH+YHYL Q DGNSLSDIEHEQQR+NESSRYSHLMN+CR
Sbjct: 121 MRLWIGGYDLGCVLSLLLLYGRHRYHYLTQGDGNSLSDIEHEQQRSNESSRYSHLMNKCR 180

Query: 181 TSLDLFFAIWFVMGNLWAFDSRLASFQRAPKLHLLCSFLLVWNAICYSFPFILFLLLCCC 240
           TSLDLFFAIWFVMGNLW FDSRLASFQRAPKLHLLCSFLLVWNAICYSFPFILFLLLCCC
Sbjct: 181 TSLDLFFAIWFVMGNLWVFDSRLASFQRAPKLHLLCSFLLVWNAICYSFPFILFLLLCCC 240

Query: 241 VPLISSLTGYNINTGSTEKGASDDQISQLPCWRYKAVEANINPRSQLDNSNTGLLKEDPE 300
           VPL SSL GYNIN GSTEKGA DDQISQLPCWRYKAVE NINP SQ DNSNTGL KEDPE
Sbjct: 241 VPLTSSLIGYNINMGSTEKGALDDQISQLPCWRYKAVETNINPGSQPDNSNTGLPKEDPE 300

Query: 301 CCICLAKYIDKEEVRQLPCSHVFHLRCVDKWLAITSSCPLCKQQLQ 347
           CCICLAKYIDKEEVRQLPCSHVFHL+CVDKWLAITSSCPLCKQQLQ
Sbjct: 301 CCICLAKYIDKEEVRQLPCSHVFHLKCVDKWLAITSSCPLCKQQLQ 346

BLAST of CSPI07G11950 vs. NCBI nr
Match: XP_031745152.1 (E3 ubiquitin-protein ligase At4g11680 isoform X2 [Cucumis sativus] >KGN44654.2 hypothetical protein Csa_016328 [Cucumis sativus])

HSP 1 Score: 656.0 bits (1691), Expect = 1.8e-184
Identity = 314/315 (99.68%), Postives = 315/315 (100.00%), Query Frame = 0

Query: 33  MADDIRNEASDSVPPSFWVKMAMKISRARWFIFLRRVFHYQNGSRSDLGPNPFNSGSWMA 92
           MADDIRNEASDSVPPSFWVKMAMKISRARWFIFLRRVFHYQNGSRSDLGPNPFNSGSWMA
Sbjct: 1   MADDIRNEASDSVPPSFWVKMAMKISRARWFIFLRRVFHYQNGSRSDLGPNPFNSGSWMA 60

Query: 93  MELVALLFQLIISAFTLAISQAEKPVWPMRLWIGGYDLGCVLSLLLLYGRHQYHYLMQRD 152
           MELVALLFQLIISAFTLAISQAEKPVWPMRLWIGGYDLGCVLSLLLLYGRH+YHYLMQRD
Sbjct: 61  MELVALLFQLIISAFTLAISQAEKPVWPMRLWIGGYDLGCVLSLLLLYGRHRYHYLMQRD 120

Query: 153 GNSLSDIEHEQQRTNESSRYSHLMNRCRTSLDLFFAIWFVMGNLWAFDSRLASFQRAPKL 212
           GNSLSDIEHEQQRTNESSRYSHLMNRCRTSLDLFFAIWFVMGNLWAFDSRLASFQRAPKL
Sbjct: 121 GNSLSDIEHEQQRTNESSRYSHLMNRCRTSLDLFFAIWFVMGNLWAFDSRLASFQRAPKL 180

Query: 213 HLLCSFLLVWNAICYSFPFILFLLLCCCVPLISSLTGYNINTGSTEKGASDDQISQLPCW 272
           HLLCSFLLVWNAICYSFPFILFLLLCCCVPLISSLTGYNINTGSTEKGASDDQISQLPCW
Sbjct: 181 HLLCSFLLVWNAICYSFPFILFLLLCCCVPLISSLTGYNINTGSTEKGASDDQISQLPCW 240

Query: 273 RYKAVEANINPRSQLDNSNTGLLKEDPECCICLAKYIDKEEVRQLPCSHVFHLRCVDKWL 332
           RYKAVEANINPRSQLDNSNTGLLKEDPECCICLAKYIDKEEVRQLPCSHVFHLRCVDKWL
Sbjct: 241 RYKAVEANINPRSQLDNSNTGLLKEDPECCICLAKYIDKEEVRQLPCSHVFHLRCVDKWL 300

Query: 333 AITSSCPLCKQQLQR 348
           AITSSCPLCKQQLQR
Sbjct: 301 AITSSCPLCKQQLQR 315

BLAST of CSPI07G11950 vs. TAIR 10
Match: AT5G55970.1 (RING/U-box superfamily protein )

HSP 1 Score: 424.1 bits (1089), Expect = 1.1e-118
Identity = 204/340 (60.00%), Postives = 255/340 (75.00%), Query Frame = 0

Query: 11  PSFNSDATTSFISISVSEEDGAMADDIRNEAS---DSVPPSFWVKMAMKISRARWFIFLR 70
           P  +S   T  IS S         D+    A+   +  P SF++++AMK+SRARWFIFLR
Sbjct: 9   PELSSSNITITISSSALLSSSPRGDNSHVAAANGQERSPSSFYIRLAMKVSRARWFIFLR 68

Query: 71  RVFHYQNGSRSDLGPNPFNSGSWMAMELVALLFQLIISAFTLAISQAEKPVWPMRLWIGG 130
           RVFHYQNGSRSDLG NPFNS +WM  EL+ALL QL +  FTLAIS+ E+P+WP+RLWI G
Sbjct: 69  RVFHYQNGSRSDLGSNPFNSSTWMMSELIALLVQLTVITFTLAISKEERPIWPVRLWITG 128

Query: 131 YDLGCVLSLLLLYGRHQYHYLMQRDGNSLSDIEHEQQRTNESSRYSHLMNRCRTSLDLFF 190
           YD+GC+L+L+LLYGR++   + Q +G  L D+E +QQR  E +R SHLMN+CRTSL+LFF
Sbjct: 129 YDVGCLLNLMLLYGRYRQLDINQGNGFVLGDVE-QQQRGREETRSSHLMNKCRTSLELFF 188

Query: 191 AIWFVMGNLWAFDSRLASFQRAPKLHLLCSFLLVWNAICYSFPFILFLLLCCCVPLISSL 250
           AIWFV+GN+W FDSR  SF  APKLH+LC  LL WNAICYSFPF+LFL LCC VPLISSL
Sbjct: 189 AIWFVIGNVWVFDSRFGSFHHAPKLHVLCVSLLAWNAICYSFPFLLFLFLCCLVPLISSL 248

Query: 251 TGYNINTGSTEKGASDDQISQLPCWRYKAVEANINPRSQLDNSNTGLLKEDPECCICLAK 310
            GYN+N GS+++ ASDDQIS LP W++K ++ + +      +S++  + +DPECCICLAK
Sbjct: 249 LGYNMNMGSSDRAASDDQISSLPSWKFKRIDDSASD----SDSDSATVTDDPECCICLAK 308

Query: 311 YIDKEEVRQLPCSHVFHLRCVDKWLAITSSCPLCKQQLQR 348
           Y DKEEVR+LPCSH FH +CVD+WL I S CPLCKQ L R
Sbjct: 309 YKDKEEVRKLPCSHKFHSKCVDQWLRIISCCPLCKQDLPR 343

BLAST of CSPI07G11950 vs. TAIR 10
Match: AT5G55970.2 (RING/U-box superfamily protein )

HSP 1 Score: 424.1 bits (1089), Expect = 1.1e-118
Identity = 204/340 (60.00%), Postives = 255/340 (75.00%), Query Frame = 0

Query: 11  PSFNSDATTSFISISVSEEDGAMADDIRNEAS---DSVPPSFWVKMAMKISRARWFIFLR 70
           P  +S   T  IS S         D+    A+   +  P SF++++AMK+SRARWFIFLR
Sbjct: 9   PELSSSNITITISSSALLSSSPRGDNSHVAAANGQERSPSSFYIRLAMKVSRARWFIFLR 68

Query: 71  RVFHYQNGSRSDLGPNPFNSGSWMAMELVALLFQLIISAFTLAISQAEKPVWPMRLWIGG 130
           RVFHYQNGSRSDLG NPFNS +WM  EL+ALL QL +  FTLAIS+ E+P+WP+RLWI G
Sbjct: 69  RVFHYQNGSRSDLGSNPFNSSTWMMSELIALLVQLTVITFTLAISKEERPIWPVRLWITG 128

Query: 131 YDLGCVLSLLLLYGRHQYHYLMQRDGNSLSDIEHEQQRTNESSRYSHLMNRCRTSLDLFF 190
           YD+GC+L+L+LLYGR++   + Q +G  L D+E +QQR  E +R SHLMN+CRTSL+LFF
Sbjct: 129 YDVGCLLNLMLLYGRYRQLDINQGNGFVLGDVE-QQQRGREETRSSHLMNKCRTSLELFF 188

Query: 191 AIWFVMGNLWAFDSRLASFQRAPKLHLLCSFLLVWNAICYSFPFILFLLLCCCVPLISSL 250
           AIWFV+GN+W FDSR  SF  APKLH+LC  LL WNAICYSFPF+LFL LCC VPLISSL
Sbjct: 189 AIWFVIGNVWVFDSRFGSFHHAPKLHVLCVSLLAWNAICYSFPFLLFLFLCCLVPLISSL 248

Query: 251 TGYNINTGSTEKGASDDQISQLPCWRYKAVEANINPRSQLDNSNTGLLKEDPECCICLAK 310
            GYN+N GS+++ ASDDQIS LP W++K ++ + +      +S++  + +DPECCICLAK
Sbjct: 249 LGYNMNMGSSDRAASDDQISSLPSWKFKRIDDSASD----SDSDSATVTDDPECCICLAK 308

Query: 311 YIDKEEVRQLPCSHVFHLRCVDKWLAITSSCPLCKQQLQR 348
           Y DKEEVR+LPCSH FH +CVD+WL I S CPLCKQ L R
Sbjct: 309 YKDKEEVRKLPCSHKFHSKCVDQWLRIISCCPLCKQDLPR 343

BLAST of CSPI07G11950 vs. TAIR 10
Match: AT4G26580.1 (RING/U-box superfamily protein )

HSP 1 Score: 412.9 bits (1060), Expect = 2.5e-115
Identity = 198/332 (59.64%), Postives = 245/332 (73.80%), Query Frame = 0

Query: 15  SDATTSFISISVSEEDGAMADDIRNEASDSVP-PSFWVKMAMKISRARWFIFLRRVFHYQ 74
           S A  S    S S       DD  + A +S P  SF++++AM++SRARWFIFLRRVFHYQ
Sbjct: 11  SSAINSITVSSSSSFASTQLDDNTHTAPESSPSSSFFMRLAMRVSRARWFIFLRRVFHYQ 70

Query: 75  NGSRSDLGPNPFNSGSWMAMELVALLFQLIISAFTLAISQAEKPVWPMRLWIGGYDLGCV 134
           N SRSDLG NPFNS +WM  EL+ALL Q+ +   TLA+S+ E+PVWPMRLWI GY++GC+
Sbjct: 71  NASRSDLGTNPFNSIAWMVSELIALLLQITVITSTLALSKNERPVWPMRLWITGYNVGCL 130

Query: 135 LSLLLLYGRHQYHYLMQRDGNSLSDIEHEQQRTNESSRYSHLMNRCRTSLDLFFAIWFVM 194
           L+L+LLYGR++       +  S  DIE +Q+   E++R SHLMN+CRTSL+LFFAIWFV+
Sbjct: 131 LNLMLLYGRYRQQDTSHENAFSFGDIEQQQRSREETTRCSHLMNKCRTSLELFFAIWFVI 190

Query: 195 GNLWAFDSRLASFQRAPKLHLLCSFLLVWNAICYSFPFILFLLLCCCVPLISSLTGYNIN 254
           GN+W FDSR  SF  AP LH+LC  LL WNA+CYSFPF+LFLLLCC VPL+SS  GYN+N
Sbjct: 191 GNVWVFDSRFGSFHYAPILHVLCISLLAWNALCYSFPFLLFLLLCCVVPLVSSFLGYNMN 250

Query: 255 TGSTEKGASDDQISQLPCWRYKAVEANINPRSQLDNSNTGLLKEDPECCICLAKYIDKEE 314
            GS+EKGASDDQIS LP W+YK ++         + S++     DPECCICLAKY +KEE
Sbjct: 251 VGSSEKGASDDQISSLPSWKYKLID---------ETSDSSQANNDPECCICLAKYKEKEE 310

Query: 315 VRQLPCSHVFHLRCVDKWLAITSSCPLCKQQL 346
           VR+LPCSH FHL+CVD+WL I S CPLCKQ L
Sbjct: 311 VRKLPCSHRFHLKCVDQWLRIISCCPLCKQDL 333

BLAST of CSPI07G11950 vs. TAIR 10
Match: AT4G26580.2 (RING/U-box superfamily protein )

HSP 1 Score: 412.9 bits (1060), Expect = 2.5e-115
Identity = 198/332 (59.64%), Postives = 245/332 (73.80%), Query Frame = 0

Query: 15  SDATTSFISISVSEEDGAMADDIRNEASDSVP-PSFWVKMAMKISRARWFIFLRRVFHYQ 74
           S A  S    S S       DD  + A +S P  SF++++AM++SRARWFIFLRRVFHYQ
Sbjct: 11  SSAINSITVSSSSSFASTQLDDNTHTAPESSPSSSFFMRLAMRVSRARWFIFLRRVFHYQ 70

Query: 75  NGSRSDLGPNPFNSGSWMAMELVALLFQLIISAFTLAISQAEKPVWPMRLWIGGYDLGCV 134
           N SRSDLG NPFNS +WM  EL+ALL Q+ +   TLA+S+ E+PVWPMRLWI GY++GC+
Sbjct: 71  NASRSDLGTNPFNSIAWMVSELIALLLQITVITSTLALSKNERPVWPMRLWITGYNVGCL 130

Query: 135 LSLLLLYGRHQYHYLMQRDGNSLSDIEHEQQRTNESSRYSHLMNRCRTSLDLFFAIWFVM 194
           L+L+LLYGR++       +  S  DIE +Q+   E++R SHLMN+CRTSL+LFFAIWFV+
Sbjct: 131 LNLMLLYGRYRQQDTSHENAFSFGDIEQQQRSREETTRCSHLMNKCRTSLELFFAIWFVI 190

Query: 195 GNLWAFDSRLASFQRAPKLHLLCSFLLVWNAICYSFPFILFLLLCCCVPLISSLTGYNIN 254
           GN+W FDSR  SF  AP LH+LC  LL WNA+CYSFPF+LFLLLCC VPL+SS  GYN+N
Sbjct: 191 GNVWVFDSRFGSFHYAPILHVLCISLLAWNALCYSFPFLLFLLLCCVVPLVSSFLGYNMN 250

Query: 255 TGSTEKGASDDQISQLPCWRYKAVEANINPRSQLDNSNTGLLKEDPECCICLAKYIDKEE 314
            GS+EKGASDDQIS LP W+YK ++         + S++     DPECCICLAKY +KEE
Sbjct: 251 VGSSEKGASDDQISSLPSWKYKLID---------ETSDSSQANNDPECCICLAKYKEKEE 310

Query: 315 VRQLPCSHVFHLRCVDKWLAITSSCPLCKQQL 346
           VR+LPCSH FHL+CVD+WL I S CPLCKQ L
Sbjct: 311 VRKLPCSHRFHLKCVDQWLRIISCCPLCKQDL 333

BLAST of CSPI07G11950 vs. TAIR 10
Match: AT4G32600.1 (RING/U-box superfamily protein )

HSP 1 Score: 188.3 bits (477), Expect = 1.0e-47
Identity = 111/306 (36.27%), Postives = 162/306 (52.94%), Query Frame = 0

Query: 83  NPFNSGSWMAMELVALLFQLIISAFTLAISQAEKPVWPMRLWIGGYDLGCVLSLLLLYGR 142
           +P NSG W+++EL   + Q+I +   L++S+ E P  P+  WI GY  GCV +L LLY R
Sbjct: 105 SPLNSGLWISIELFLTVGQIIAAIVVLSLSKHEHPRAPLFTWIVGYACGCVATLPLLYWR 164

Query: 143 HQYHYLMQRDGNS--------------------LSDIEHEQQRTNES--SRYSHLMNRCR 202
           + YH     + +S                     S+ +  Q  T  S  SRY   ++  R
Sbjct: 165 Y-YHSNQASEQDSGQHRPNLNVAAGPFAFSISRTSEADGRQTNTTSSRGSRYPGFISAAR 224

Query: 203 ---------TSLDLFFAIWFVMGNLWAFDSRLASFQRAPKLHLLCSFLLVWNAICYSFPF 262
                     +LD FFA+WFV+GN+W F    +S   AP L+ LC   L ++ I Y+ PF
Sbjct: 225 LKVIVEYFKMALDCFFAVWFVVGNVWIFGGH-SSAAEAPNLYRLCLVFLTFSCIGYAMPF 284

Query: 263 ILFLLLCCCVPLISSLTGYNINTGSTEKGASDDQISQLPCWRYKAVEANINPRSQLDNSN 322
           IL   +CCC+P I S+ GY  +  +  +GA+ + I+ LP  ++K  ++  N      +++
Sbjct: 285 ILCTTICCCLPCIISILGYREDL-TQPRGATPESINALPTHKFKLKKSRSNGDDNGSSTS 344

Query: 323 TG------------LLKEDPECCICLAKYIDKEEVRQLPCSHVFHLRCVDKWLAITSSCP 346
            G            +  ED  CCICLAKY + EE+R+LPCSH FH  CVDKWL I +SCP
Sbjct: 345 EGGVVAAGTDNERAISGEDAVCCICLAKYANNEELRELPCSHFFHKECVDKWLKINASCP 404

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q93Z922.1e-2628.41E3 ubiquitin-protein ligase At4g11680 OS=Arabidopsis thaliana OX=3702 GN=At4g116... [more]
Q9LN716.2e-2626.56E3 ubiquitin-protein ligase At1g12760 OS=Arabidopsis thaliana OX=3702 GN=At1g127... [more]
Q8LDB88.1e-2626.16E3 ubiquitin-protein ligase At1g63170 OS=Arabidopsis thaliana OX=3702 GN=At1g631... [more]
Q8GUU24.0e-2527.61E3 ubiquitin protein ligase RIE1 OS=Arabidopsis thaliana OX=3702 GN=RIE1 PE=2 SV... [more]
Q9ZT491.9e-1138.64Probable E3 ubiquitin-protein ligase ATL45 OS=Arabidopsis thaliana OX=3702 GN=AT... [more]
Match NameE-valueIdentityDescription
A0A0A0K4H01.6e-20299.71RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G278230 P... [more]
A0A1S3CI941.6e-19495.97E3 ubiquitin-protein ligase At4g11680 isoform X1 OS=Cucumis melo OX=3656 GN=LOC1... [more]
A0A5D3CCB03.9e-18886.91E3 ubiquitin-protein ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf... [more]
A0A1S3CIA11.4e-17795.87E3 ubiquitin-protein ligase At4g11680 isoform X2 OS=Cucumis melo OX=3656 GN=LOC1... [more]
A0A6J1CCU06.0e-17386.74E3 ubiquitin-protein ligase At1g63170 OS=Momordica charantia OX=3673 GN=LOC11101... [more]
Match NameE-valueIdentityDescription
XP_011659087.13.3e-20299.71E3 ubiquitin-protein ligase At4g11680 isoform X1 [Cucumis sativus][more]
XP_008463025.13.3e-19495.97PREDICTED: E3 ubiquitin-protein ligase At4g11680 isoform X1 [Cucumis melo][more]
KAA0048251.18.0e-18886.91E3 ubiquitin-protein ligase [Cucumis melo var. makuwa] >TYK08006.1 E3 ubiquitin-... [more]
XP_038899145.11.5e-18691.91E3 ubiquitin-protein ligase At4g11680-like [Benincasa hispida][more]
XP_031745152.11.8e-18499.68E3 ubiquitin-protein ligase At4g11680 isoform X2 [Cucumis sativus] >KGN44654.2 h... [more]
Match NameE-valueIdentityDescription
AT5G55970.11.1e-11860.00RING/U-box superfamily protein [more]
AT5G55970.21.1e-11860.00RING/U-box superfamily protein [more]
AT4G26580.12.5e-11559.64RING/U-box superfamily protein [more]
AT4G26580.22.5e-11559.64RING/U-box superfamily protein [more]
AT4G32600.11.0e-4736.27RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typePFAMPF17123zf-RING_11coord: 300..325
e-value: 4.9E-9
score: 35.8
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 249..327
e-value: 1.2E-9
score: 39.7
NoneNo IPR availablePANTHERPTHR46225:SF1RING/U-BOX SUPERFAMILY PROTEINcoord: 26..326
NoneNo IPR availablePANTHERPTHR46225C3H4 TYPE ZINC FINGER PROTEINcoord: 26..326
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 294..325

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI07G11950.2CSPI07G11950.2mRNA
CSPI07G11950.1CSPI07G11950.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0008270 zinc ion binding