CSPI07G10990 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI07G10990
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Defensin-like protein 3
Location	Chr7: 9128812 .. 9129107 (+)
RNA-Seq Expression	CSPI07G10990
Synteny	CSPI07G10990

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAGGAGGATGGAAAAAACTCACATTTGTGGCATTTATCTTTCTGCTAGCCATTGCTTCTGGTACTCAATCTGATGCCATTATTTCATGTCATTTCCACAATAAAATGTTGTGTTGTTGTTACTTGCAGGATGGATGATGGTGGAGACGGCAGGAAAGATTTGCTTCAAGCAAAGCCGGTCGTTCAAGGGAAAGTGTATTTCAAATGATGAATGTGTTAAAGCTTGCGATACCGACATGTGGTATGACGGATTTTGCTCGAATGGTTTATGCATTTGTACCGTCATTTGTTGA

mRNA sequence

ATGGCAGGAGGATGGAAAAAACTCACATTTGTGGCATTTATCTTTCTGCTAGCCATTGCTTCTGGATGGATGATGGTGGAGACGGCAGGAAAGATTTGCTTCAAGCAAAGCCGGTCGTTCAAGGGAAAGTGTATTTCAAATGATGAATGTGTTAAAGCTTGCGATACCGACATGTGGTATGACGGATTTTGCTCGAATGGTTTATGCATTTGTACCGTCATTTGTTGA

Coding sequence (CDS)

ATGGCAGGAGGATGGAAAAAACTCACATTTGTGGCATTTATCTTTCTGCTAGCCATTGCTTCTGGATGGATGATGGTGGAGACGGCAGGAAAGATTTGCTTCAAGCAAAGCCGGTCGTTCAAGGGAAAGTGTATTTCAAATGATGAATGTGTTAAAGCTTGCGATACCGACATGTGGTATGACGGATTTTGCTCGAATGGTTTATGCATTTGTACCGTCATTTGTTGA

Protein sequence

MAGGWKKLTFVAFIFLLAIASGWMMVETAGKICFKQSRSFKGKCISNDECVKACDTDMWYDGFCSNGLCICTVIC*

Homology

[Close]

BLAST of CSPI07G10990 vs. ExPASy Swiss-Prot
Match: P82781 (Putative defensin-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=LCR71 PE=3 SV=2)

HSP 1 Score: 48.5 bits (114), Expect = 3.8e-05
Identity = 21/62 (33.87%), Postives = 31/62 (50.00%), Query Frame = 0


Query: 16 LLAIASGWMMVETAGKICFKQSRSFKGKCISNDECVKACDTDMWYDGFCSNGL--CICTV 75
          ++ + + W  V    +IC  +S+ FKG C+S D C   C T+ + DG C   L  C C  
Sbjct: 1  MMILVNAWGSVTVEARICKSRSQKFKGPCVSEDNCANVCHTEGFPDGDCDGLLRRCYCNT 60

[Close]

BLAST of CSPI07G10990 vs. ExPASy Swiss-Prot
Match: Q6K209 (Defensin-like protein CAL1 OS=Oryza sativa subsp. japonica OX=39947 GN=CAL1 PE=1 SV=1)

HSP 1 Score: 44.7 bits (104), Expect = 5.5e-04
Identity = 26/70 (37.14%), Postives = 36/70 (51.43%), Query Frame = 0


Query: 10 FVAFIFLLAIASGWMMVETAGKICFKQSRSFKGKCISNDECVKACDTDMWYDGFC-SNGL 69
          F+    L+A   G   V  A + C  QS  FKG C+S++ C   C T+ + DG C S+GL
Sbjct: 12 FLLLAILVATEMGTTKVAEA-RHCLSQSHRFKGMCVSSNNCANVCRTESFPDGECKSHGL 71

Query: 70 ---CICTVIC 76
             C C  +C
Sbjct: 72 ERKCFCKKVC 80

[Close]

BLAST of CSPI07G10990 vs. ExPASy TrEMBL
Match: A0A0A0K447 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G239060 PE=4 SV=1)

HSP 1 Score: 164.1 bits (414), Expect = 2.3e-37
Identity = 75/75 (100.00%), Postives = 75/75 (100.00%), Query Frame = 0


Query: 1  MAGGWKKLTFVAFIFLLAIASGWMMVETAGKICFKQSRSFKGKCISNDECVKACDTDMWY 60
          MAGGWKKLTFVAFIFLLAIASGWMMVETAGKICFKQSRSFKGKCISNDECVKACDTDMWY
Sbjct: 1  MAGGWKKLTFVAFIFLLAIASGWMMVETAGKICFKQSRSFKGKCISNDECVKACDTDMWY 60

Query: 61 DGFCSNGLCICTVIC 76
          DGFCSNGLCICTVIC
Sbjct: 61 DGFCSNGLCICTVIC 75

[Close]

BLAST of CSPI07G10990 vs. ExPASy TrEMBL
Match: A0A5D3BCI9 (Defensin-like protein 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold986G00530 PE=4 SV=1)

HSP 1 Score: 137.5 bits (345), Expect = 2.3e-29
Identity = 64/75 (85.33%), Postives = 67/75 (89.33%), Query Frame = 0


Query: 1  MAGGWKKLTFVAFIFLLAIASGWMMVETAGKICFKQSRSFKGKCISNDECVKACDTDMWY 60
          MAG WKKLTFVAF+F L+IASGWMM ET GKICFKQS SFKGKC SNDECVKACD DMWY
Sbjct: 1  MAGRWKKLTFVAFVF-LSIASGWMMEETEGKICFKQSPSFKGKCTSNDECVKACDIDMWY 60

Query: 61 DGFCSNGLCICTVIC 76
          +G CSNGLCICTVIC
Sbjct: 61 NGVCSNGLCICTVIC 74

[Close]

BLAST of CSPI07G10990 vs. ExPASy TrEMBL
Match: W5RQN1 (Defensin-like protein 3 OS=Citrullus lanatus OX=3654 GN=PDF2.3 PE=2 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 1.8e-13
Identity = 41/77 (53.25%), Postives = 55/77 (71.43%), Query Frame = 0


Query: 1  MAGG--WKKLTFVAFIFLLAIASGWMMVETAGKICFKQSRSFKGKCISNDECVKACDTDM 60
          MAG   W+KLT V FIF L++ASGWM+ ET G+ C++ S SFKG+C S+++C+  C    
Sbjct: 1  MAGSEEWRKLTIVLFIF-LSLASGWMVAETEGRACYRPSPSFKGRCSSDEDCIGVC---- 60

Query: 61 WYDGFCSNGLCICTVIC 76
            DGFC++G CICTV C
Sbjct: 61 --DGFCNHGSCICTVPC 70

[Close]

BLAST of CSPI07G10990 vs. ExPASy TrEMBL
Match: A0A5A7V3H5 (Defensin-like protein 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold986G00550 PE=4 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 7.0e-10
Identity = 37/73 (50.68%), Postives = 47/73 (64.38%), Query Frame = 0


Query: 4  GWKKLTFVAFIFLLAIASGWMMVETAGKICFKQSRSFKGKCISNDECVKAC-DTDMWYDG 63
          G +KLT V  IFL+  ASGWM+V+T G  C+K S +F+G C S +EC + C   + W DG
Sbjct: 5  GLRKLTVVVLIFLV-FASGWMVVQTEGLTCYKPSTAFQGTCNSAEECGRVCFYQEGWDDG 64

Query: 64 FCSNGLCICTVIC 76
          FCS   CICT  C
Sbjct: 65 FCSGDKCICTEKC 76

[Close]

BLAST of CSPI07G10990 vs. ExPASy TrEMBL
Match: A0A5D3BEY4 (Defensin-like protein 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold986G00520 PE=4 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 2.0e-09
Identity = 33/72 (45.83%), Postives = 47/72 (65.28%), Query Frame = 0


Query: 4  GWKKLTFVAFIFLLAIASGWMMVETAGKICFKQSRSFKGKCISNDECVKACDTDMWYDGF 63
          G +KLT V  + LL++ASGWM+ ET G++CF+   + KG C S+++C+  C      DGF
Sbjct: 5  GRRKLT-VVLVILLSVASGWMVAETEGRVCFRPRPTLKGVCSSDEDCIAVC------DGF 64

Query: 64 CSNGLCICTVIC 76
          CS G C+C V C
Sbjct: 65 CSLGKCMCAVPC 69

[Close]

BLAST of CSPI07G10990 vs. NCBI nr
Match: KGN44293.1 (hypothetical protein Csa_016568 [Cucumis sativus])

HSP 1 Score: 164.1 bits (414), Expect = 4.8e-37
Identity = 75/75 (100.00%), Postives = 75/75 (100.00%), Query Frame = 0


Query: 1  MAGGWKKLTFVAFIFLLAIASGWMMVETAGKICFKQSRSFKGKCISNDECVKACDTDMWY 60
          MAGGWKKLTFVAFIFLLAIASGWMMVETAGKICFKQSRSFKGKCISNDECVKACDTDMWY
Sbjct: 1  MAGGWKKLTFVAFIFLLAIASGWMMVETAGKICFKQSRSFKGKCISNDECVKACDTDMWY 60

Query: 61 DGFCSNGLCICTVIC 76
          DGFCSNGLCICTVIC
Sbjct: 61 DGFCSNGLCICTVIC 75

[Close]

BLAST of CSPI07G10990 vs. NCBI nr
Match: KAA0062794.1 (defensin-like protein 3 [Cucumis melo var. makuwa] >TYJ96759.1 defensin-like protein 3 [Cucumis melo var. makuwa])

HSP 1 Score: 137.5 bits (345), Expect = 4.8e-29
Identity = 64/75 (85.33%), Postives = 67/75 (89.33%), Query Frame = 0


Query: 1  MAGGWKKLTFVAFIFLLAIASGWMMVETAGKICFKQSRSFKGKCISNDECVKACDTDMWY 60
          MAG WKKLTFVAF+F L+IASGWMM ET GKICFKQS SFKGKC SNDECVKACD DMWY
Sbjct: 1  MAGRWKKLTFVAFVF-LSIASGWMMEETEGKICFKQSPSFKGKCTSNDECVKACDIDMWY 60

Query: 61 DGFCSNGLCICTVIC 76
          +G CSNGLCICTVIC
Sbjct: 61 NGVCSNGLCICTVIC 74

[Close]

BLAST of CSPI07G10990 vs. NCBI nr
Match: XP_038887289.1 (defensin-like protein 2 [Benincasa hispida])

HSP 1 Score: 119.8 bits (299), Expect = 1.0e-23
Identity = 56/76 (73.68%), Postives = 64/76 (84.21%), Query Frame = 0


Query: 1  MAG-GWKKLTFVAFIFLLAIASGWMMVETAGKICFKQSRSFKGKCISNDECVKACDTDMW 60
          MAG GW+KLT V FIF LA+ASGWM+VET GK CFKQS SF+G+C SN+ECV ACD DMW
Sbjct: 1  MAGEGWRKLTVVLFIF-LALASGWMVVETEGKTCFKQSPSFRGQCTSNNECVGACDNDMW 60

Query: 61 YDGFCSNGLCICTVIC 76
          Y+G+CSNGLCIC V C
Sbjct: 61 YNGYCSNGLCICAVHC 75

[Close]

BLAST of CSPI07G10990 vs. NCBI nr
Match: AHA50469.1 (defensin-like protein 3 [Citrullus lanatus])

HSP 1 Score: 84.7 bits (208), Expect = 3.7e-13
Identity = 41/77 (53.25%), Postives = 55/77 (71.43%), Query Frame = 0


Query: 1  MAGG--WKKLTFVAFIFLLAIASGWMMVETAGKICFKQSRSFKGKCISNDECVKACDTDM 60
          MAG   W+KLT V FIF L++ASGWM+ ET G+ C++ S SFKG+C S+++C+  C    
Sbjct: 1  MAGSEEWRKLTIVLFIF-LSLASGWMVAETEGRACYRPSPSFKGRCSSDEDCIGVC---- 60

Query: 61 WYDGFCSNGLCICTVIC 76
            DGFC++G CICTV C
Sbjct: 61 --DGFCNHGSCICTVPC 70

[Close]

BLAST of CSPI07G10990 vs. NCBI nr
Match: XP_031744629.1 (defensin-1-like [Cucumis sativus])

HSP 1 Score: 82.8 bits (203), Expect = 1.4e-12
Identity = 38/72 (52.78%), Postives = 50/72 (69.44%), Query Frame = 0


Query: 4  GWKKLTFVAFIFLLAIASGWMMVETAGKICFKQSRSFKGKCISNDECVKACDTDMWYDGF 63
          GW+KLT V FI LL++ASGWM+ ET G++C++ S   KG C SND+C+  C      DGF
Sbjct: 5  GWRKLTVVLFI-LLSLASGWMVAETEGRVCYRPSPRLKGVCSSNDDCIAVC------DGF 64

Query: 64 CSNGLCICTVIC 76
          CS+G C+C V C
Sbjct: 65 CSHGKCMCEVPC 69

[Close]

BLAST of CSPI07G10990 vs. TAIR 10
Match: AT2G02120.1 (Scorpion toxin-like knottin superfamily protein )

HSP 1 Score: 43.5 bits (101), Expect = 8.8e-05
Identity = 22/70 (31.43%), Postives = 35/70 (50.00%), Query Frame = 0


Query: 8  LTFVAFIFLLAIASGWMMVETAGKICFKQSRSFKGKCISNDECVKACDTDMWYDGFCS-- 67
          ++ V F+ ++ +A+G   V    + C  QS+ FKGKC+S+  C   C  + +  G C   
Sbjct: 8  ISAVLFLVMIFVATGMGPVTVEARTCASQSQRFKGKCVSDTNCENVCHNEGFPGGDCRGF 67

Query: 68 NGLCICTVIC 76
             C CT  C
Sbjct: 68 RRRCFCTRNC 77

[Close]

BLAST of CSPI07G10990 vs. TAIR 10
Match: AT2G02100.1 (low-molecular-weight cysteine-rich 69 )

HSP 1 Score: 41.2 bits (95), Expect = 4.4e-04
Identity = 21/70 (30.00%), Postives = 32/70 (45.71%), Query Frame = 0


Query: 8  LTFVAFIFLLAIASGWMMVETAGKICFKQSRSFKGKCISNDECVKACDTDMWYDGFCS-- 67
          ++ V  +F++ +A+G   V    + C  QS  FKG C+S   C   C  + +  G C   
Sbjct: 8  ISAVLIMFMIFVATGMGPVTVEARTCESQSHRFKGTCVSASNCANVCHNEGFVGGNCRGF 67

Query: 68 NGLCICTVIC 76
             C CT  C
Sbjct: 68 RRRCFCTRHC 77

The following BLAST results are available for this feature:

BLAST of CSPI07G10990 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P82781	3.8e-05	33.87	Putative defensin-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=LCR71 PE=3 S...	[more]
Q6K209	5.5e-04	37.14	Defensin-like protein CAL1 OS=Oryza sativa subsp. japonica OX=39947 GN=CAL1 PE=1...	[more]

BLAST of CSPI07G10990 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K447	2.3e-37	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G239060 PE=4 SV=1	[more]
A0A5D3BCI9	2.3e-29	85.33	Defensin-like protein 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold...	[more]
W5RQN1	1.8e-13	53.25	Defensin-like protein 3 OS=Citrullus lanatus OX=3654 GN=PDF2.3 PE=2 SV=1	[more]
A0A5A7V3H5	7.0e-10	50.68	Defensin-like protein 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold...	[more]
A0A5D3BEY4	2.0e-09	45.83	Defensin-like protein 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold...	[more]

BLAST of CSPI07G10990 vs. NCBI nr


Match Name	E-value	Identity	Description
KGN44293.1	4.8e-37	100.00	hypothetical protein Csa_016568 [Cucumis sativus]	[more]
KAA0062794.1	4.8e-29	85.33	defensin-like protein 3 [Cucumis melo var. makuwa] >TYJ96759.1 defensin-like pro...	[more]
XP_038887289.1	1.0e-23	73.68	defensin-like protein 2 [Benincasa hispida]	[more]
AHA50469.1	3.7e-13	53.25	defensin-like protein 3 [Citrullus lanatus]	[more]
XP_031744629.1	1.4e-12	52.78	defensin-1-like [Cucumis sativus]	[more]

BLAST of CSPI07G10990 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G02120.1	8.8e-05	31.43	Scorpion toxin-like knottin superfamily protein	[more]
AT2G02100.1	4.4e-04	30.00	low-molecular-weight cysteine-rich 69	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PFAM	PF00304	Gamma-thionin	coord: 31..75 e-value: 4.2E-7 score: 30.1
IPR036574	Knottin, scorpion toxin-like superfamily	GENE3D	3.30.30.10		coord: 31..75 e-value: 1.1E-9 score: 40.4
IPR036574	Knottin, scorpion toxin-like superfamily	SUPERFAMILY	57095	Scorpion toxin-like	coord: 31..75
IPR008176	Defensin, plant	PROSITE	PS00940	GAMMA_THIONIN	coord: 31..54

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI07G10990.1	CSPI07G10990.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006952	defense response