CSPI07G10300 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI07G10300
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	major latex allergen Hev b 5-like
Location	Chr7: 8420404 .. 8421505 (-)
RNA-Seq Expression	CSPI07G10300
Synteny	CSPI07G10300

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CCTTTGCCCATCCACACCAATTACAATCTCAAAATCTCTTCTTTCTCTTCTTCCAAATCTTTCTCTCCTTAACCGGAGGGAAAGAAAACTTAATCGGTTGTTTTTCATACATATAGATAATGGGTGCCTGCGCTACCAAGCCTAAAGTTAAGGCCGATGATTTGGCTGCAATCCCTGCACCCGAACCTATTGATCATGACATTGTGAAGAACAACGAGGAAGTCAAATTCGTGGAAGAGGAAAAGAAGAGTGATCAGACCGATGAAAAAAAGAAGGAAAACAACGAGAAAGTTGTCGTCAAGGAGAAGAAGCCCCCGTCTCTCGCCTCCTTGCTCGTTGAGGTATACTTTATCTAATTTAATAGATTGAATATTTGAAGGCTTTCAAACAAAGTAGGAGATCTTGTTTTGAAAATTGTTTATGTAATTTATTTATTATGATTAGAAACCCACAAACTATAGAAGATAACTTGGGAGTAAATAAAGAACAAAGGTAAGTGTAACTTAGTGATTAGATATTTAGGTAAACGATACAAATATAGTTTGAGATGCTATAATAAATAAACATAGAATAATCATCTATGAAAATATAATTCCACTTGATAGAATATCTGGGATGTAACATTGCATATTGCATATTTGAAAGTAATTTTCAAATTATCAAAATCTTAAACATGCATTTAGGGTTCTTTTTATGAGTTTTTGAGTTCTTGATAAGGAAATTATTAAGCATGTGAATAAAATAGAAAAAAAATTGCACCTTCTTTTATAGAAGGAAGCCGCTCCGGCTGTAGAAGTTTCCGGCATACCGAAGGCCGAGGAAAAGCCGAAGGCGAAAGAAGTGGCAGCGCCAACTATTGAAGCAGAACTGAAGAAAAGCGAAGAGAAACCAAAAGAAAAGGATGAGAAAAGCGTATGATGATTTGAAAGAAGAAAATAAGGCGAAGCGCTCTTTATTTGAAATATAATTTTCTCTTCTGAAAAGCTATTCATTATTCAGTATGTTGTGCAAAGATTGGTAATCATTTCTAAGGTGTGGAGGGGTCTATGTTTCCTTTATCTATTAATATTCACTTTAATGAAATATTTAAAACTTCTTTTAG

mRNA sequence

CCTTTGCCCATCCACACCAATTACAATCTCAAAATCTCTTCTTTCTCTTCTTCCAAATCTTTCTCTCCTTAACCGGAGGGAAAGAAAACTTAATCGGTTGTTTTTCATACATATAGATAATGGGTGCCTGCGCTACCAAGCCTAAAGTTAAGGCCGATGATTTGGCTGCAATCCCTGCACCCGAACCTATTGATCATGACATTGTGAAGAACAACGAGGAAGTCAAATTCGTGGAAGAGGAAAAGAAGAGTGATCAGACCGATGAAAAAAAGAAGGAAAACAACGAGAAAGTTGTCGTCAAGGAGAAGAAGCCCCCGTCTCTCGCCTCCTTGCTCGTTGAGAAGGAAGCCGCTCCGGCTGTAGAAGTTTCCGGCATACCGAAGGCCGAGGAAAAGCCGAAGGCGAAAGAAGTGGCAGCGCCAACTATTGAAGCAGAACTGAAGAAAAGCGAAGAGAAACCAAAAGAAAAGGATGAGAAAAGCGTATGATGATTTGAAAGAAGAAAATAAGGCGAAGCGCTCTTTATTTGAAATATAATTTTCTCTTCTGAAAAGCTATTCATTATTCAGTATGTTGTGCAAAGATTGGTAATCATTTCTAAGGTGTGGAGGGGTCTATGTTTCCTTTATCTATTAATATTCACTTTAATGAAATATTTAAAACTTCTTTTAG

Coding sequence (CDS)

ATGGGTGCCTGCGCTACCAAGCCTAAAGTTAAGGCCGATGATTTGGCTGCAATCCCTGCACCCGAACCTATTGATCATGACATTGTGAAGAACAACGAGGAAGTCAAATTCGTGGAAGAGGAAAAGAAGAGTGATCAGACCGATGAAAAAAAGAAGGAAAACAACGAGAAAGTTGTCGTCAAGGAGAAGAAGCCCCCGTCTCTCGCCTCCTTGCTCGTTGAGAAGGAAGCCGCTCCGGCTGTAGAAGTTTCCGGCATACCGAAGGCCGAGGAAAAGCCGAAGGCGAAAGAAGTGGCAGCGCCAACTATTGAAGCAGAACTGAAGAAAAGCGAAGAGAAACCAAAAGAAAAGGATGAGAAAAGCGTATGA

Protein sequence

MGACATKPKVKADDLAAIPAPEPIDHDIVKNNEEVKFVEEEKKSDQTDEKKKENNEKVVVKEKKPPSLASLLVEKEAAPAVEVSGIPKAEEKPKAKEVAAPTIEAELKKSEEKPKEKDEKSV*

Homology

[Close]

BLAST of CSPI07G10300 vs. ExPASy TrEMBL
Match: A0A0A0K3E6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G230950 PE=4 SV=1)

HSP 1 Score: 216.1 bits (549), Expect = 8.3e-53
Identity = 122/122 (100.00%), Postives = 122/122 (100.00%), Query Frame = 0


Query: 1   MGACATKPKVKADDLAAIPAPEPIDHDIVKNNEEVKFVEEEKKSDQTDEKKKENNEKVVV 60
           MGACATKPKVKADDLAAIPAPEPIDHDIVKNNEEVKFVEEEKKSDQTDEKKKENNEKVVV
Sbjct: 1   MGACATKPKVKADDLAAIPAPEPIDHDIVKNNEEVKFVEEEKKSDQTDEKKKENNEKVVV 60

Query: 61  KEKKPPSLASLLVEKEAAPAVEVSGIPKAEEKPKAKEVAAPTIEAELKKSEEKPKEKDEK 120
           KEKKPPSLASLLVEKEAAPAVEVSGIPKAEEKPKAKEVAAPTIEAELKKSEEKPKEKDEK
Sbjct: 61  KEKKPPSLASLLVEKEAAPAVEVSGIPKAEEKPKAKEVAAPTIEAELKKSEEKPKEKDEK 120

Query: 121 SV 123
           SV
Sbjct: 121 SV 122

[Close]

BLAST of CSPI07G10300 vs. ExPASy TrEMBL
Match: A0A1S3BGT9 (major latex allergen Hev b 5-like OS=Cucumis melo OX=3656 GN=LOC103489688 PE=4 SV=1)

HSP 1 Score: 174.1 bits (440), Expect = 3.6e-40
Identity = 101/123 (82.11%), Postives = 111/123 (90.24%), Query Frame = 0


Query: 1   MGACATKPKVKADDLAAIPAPEPIDHDIVKNNEEVKFVEEEKKSDQTDEKKKENNEKVVV 60
           MGACATKPKVK DDLAAIPAPEPIDHDIVKNNEE+K VEEEKK DQTDEKKKE+NEKV+V
Sbjct: 1   MGACATKPKVKTDDLAAIPAPEPIDHDIVKNNEEIKVVEEEKKVDQTDEKKKEDNEKVIV 60

Query: 61  KEKKPPSLASLLVEKEAA-PAVEVSGIPKAEEKPKAKEVAAPTIEAELKKSEEKPKEKDE 120
           +++K PSLASLLVEKE A P VEVS IPKA+EKPK +EV APT EAE K+SEEKPKEK+E
Sbjct: 61  EKEKSPSLASLLVEKETAPPVVEVSCIPKADEKPKVEEVVAPTNEAEAKESEEKPKEKNE 120

Query: 121 KSV 123
           KSV
Sbjct: 121 KSV 123

[Close]

BLAST of CSPI07G10300 vs. ExPASy TrEMBL
Match: A0A5A7TGB5 (Major latex allergen Hev b 5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold306G00890 PE=4 SV=1)

HSP 1 Score: 118.6 bits (296), Expect = 1.8e-23
Identity = 63/74 (85.14%), Postives = 69/74 (93.24%), Query Frame = 0


Query: 1  MGACATKPKVKADDLAAIPAPEPIDHDIVKNNEEVKFVEEEKKSDQTDEKKKENNEKVVV 60
          MGACATKPKVK DDLA IPAPEPIDHDIVKNNEE+K VEEEKK DQTDEKKKE+NEKV+V
Sbjct: 1  MGACATKPKVKTDDLATIPAPEPIDHDIVKNNEEIKVVEEEKKVDQTDEKKKEDNEKVIV 60

Query: 61 KEKKPPSLASLLVE 75
          +++K PSLASLLVE
Sbjct: 61 EKEKSPSLASLLVE 74

[Close]

BLAST of CSPI07G10300 vs. ExPASy TrEMBL
Match: A0A6J1EUI1 (patellin-1-like OS=Cucurbita moschata OX=3662 GN=LOC111437877 PE=4 SV=1)

HSP 1 Score: 109.0 bits (271), Expect = 1.4e-20
Identity = 78/122 (63.93%), Postives = 90/122 (73.77%), Query Frame = 0


Query: 1   MGACATKPKVKADDLAAIPAPEPIDHDIVKNNEEVKFVEEEKKSDQT-DEKKKENNEKVV 60
           MGACATKPK K  D A IPAPEP+D D+VKN EEVK VE+EK+ D   DEKKK++ EKVV
Sbjct: 1   MGACATKPKAKV-DTAVIPAPEPVD-DMVKNTEEVKVVEDEKRVDDVIDEKKKDDTEKVV 60

Query: 61  VKEKKPPSLASLLVEKEAAPAVEVSGIPKAEEKPKAKEVAAPTIEAELKKSEEKPKEKDE 120
           V+E KPPSL SLLVEKEA    EV  + KAEE+P  KE AA     E+KKSEEK  ++DE
Sbjct: 61  VEEAKPPSLDSLLVEKEA----EVPSVTKAEEQP-PKEAAAVAGAKEVKKSEEKAAKRDE 115

Query: 121 KS 122
           KS
Sbjct: 121 KS 115

[Close]

BLAST of CSPI07G10300 vs. ExPASy TrEMBL
Match: A0A6J1DY67 (salt stress root protein RS1-like OS=Momordica charantia OX=3673 GN=LOC111024158 PE=4 SV=1)

HSP 1 Score: 87.4 bits (215), Expect = 4.5e-14
Identity = 69/134 (51.49%), Postives = 90/134 (67.16%), Query Frame = 0


Query: 1   MGACATKPKVKADD---LAAIPAPEPIDHDIVKNNEEVKFVEEEKKSDQTDEK-KKENNE 60
           MGACATKPKVK D+    A  P PEP    ++K+ EEVK V+E+KK D T++K KKE+ +
Sbjct: 1   MGACATKPKVKEDEPYIPAPEPEPEPTLEIMMKSTEEVKVVQEDKKVDGTNQKIKKEDTK 60

Query: 61  KVVVKEKKPPSLASLLVEKEAAPAVEVSGIPKAEE-KPKAKE-------VAAPTIEAELK 120
           K+VV+E+K  SL+SLLVEK+ APAVEV    K E+ KPK +E       VAA     ++K
Sbjct: 61  KIVVEEEKAHSLSSLLVEKKEAPAVEVPSATKEEKLKPKVEEAKAEPPKVAAVPAPEQIK 120

Query: 121 KSEEKPKEKDEKSV 123
           K ++K   KDEKSV
Sbjct: 121 KCDDKATGKDEKSV 134

[Close]

BLAST of CSPI07G10300 vs. NCBI nr
Match: XP_004139808.1 (microtubule-associated protein 1B isoform X1 [Cucumis sativus])

HSP 1 Score: 216.1 bits (549), Expect = 1.7e-52
Identity = 122/122 (100.00%), Postives = 122/122 (100.00%), Query Frame = 0


Query: 1   MGACATKPKVKADDLAAIPAPEPIDHDIVKNNEEVKFVEEEKKSDQTDEKKKENNEKVVV 60
           MGACATKPKVKADDLAAIPAPEPIDHDIVKNNEEVKFVEEEKKSDQTDEKKKENNEKVVV
Sbjct: 1   MGACATKPKVKADDLAAIPAPEPIDHDIVKNNEEVKFVEEEKKSDQTDEKKKENNEKVVV 60

Query: 61  KEKKPPSLASLLVEKEAAPAVEVSGIPKAEEKPKAKEVAAPTIEAELKKSEEKPKEKDEK 120
           KEKKPPSLASLLVEKEAAPAVEVSGIPKAEEKPKAKEVAAPTIEAELKKSEEKPKEKDEK
Sbjct: 61  KEKKPPSLASLLVEKEAAPAVEVSGIPKAEEKPKAKEVAAPTIEAELKKSEEKPKEKDEK 120

Query: 121 SV 123
           SV
Sbjct: 121 SV 122

[Close]

BLAST of CSPI07G10300 vs. NCBI nr
Match: XP_031745011.1 (microtubule-associated protein 1B isoform X2 [Cucumis sativus] >KAE8646082.1 hypothetical protein Csa_016087 [Cucumis sativus])

HSP 1 Score: 209.9 bits (533), Expect = 1.2e-50
Identity = 121/122 (99.18%), Postives = 121/122 (99.18%), Query Frame = 0


Query: 1   MGACATKPKVKADDLAAIPAPEPIDHDIVKNNEEVKFVEEEKKSDQTDEKKKENNEKVVV 60
           MGACATKPKVKADDLAAIPAPEPIDHDIVKNNEEVKFVEEEKKSDQTDEKKKENNEKVVV
Sbjct: 1   MGACATKPKVKADDLAAIPAPEPIDHDIVKNNEEVKFVEEEKKSDQTDEKKKENNEKVVV 60

Query: 61  KEKKPPSLASLLVEKEAAPAVEVSGIPKAEEKPKAKEVAAPTIEAELKKSEEKPKEKDEK 120
           KEKKPPSLASLLVE EAAPAVEVSGIPKAEEKPKAKEVAAPTIEAELKKSEEKPKEKDEK
Sbjct: 61  KEKKPPSLASLLVE-EAAPAVEVSGIPKAEEKPKAKEVAAPTIEAELKKSEEKPKEKDEK 120

Query: 121 SV 123
           SV
Sbjct: 121 SV 121

[Close]

BLAST of CSPI07G10300 vs. NCBI nr
Match: XP_008447176.1 (PREDICTED: major latex allergen Hev b 5-like [Cucumis melo])

HSP 1 Score: 174.1 bits (440), Expect = 7.5e-40
Identity = 101/123 (82.11%), Postives = 111/123 (90.24%), Query Frame = 0


Query: 1   MGACATKPKVKADDLAAIPAPEPIDHDIVKNNEEVKFVEEEKKSDQTDEKKKENNEKVVV 60
           MGACATKPKVK DDLAAIPAPEPIDHDIVKNNEE+K VEEEKK DQTDEKKKE+NEKV+V
Sbjct: 1   MGACATKPKVKTDDLAAIPAPEPIDHDIVKNNEEIKVVEEEKKVDQTDEKKKEDNEKVIV 60

Query: 61  KEKKPPSLASLLVEKEAA-PAVEVSGIPKAEEKPKAKEVAAPTIEAELKKSEEKPKEKDE 120
           +++K PSLASLLVEKE A P VEVS IPKA+EKPK +EV APT EAE K+SEEKPKEK+E
Sbjct: 61  EKEKSPSLASLLVEKETAPPVVEVSCIPKADEKPKVEEVVAPTNEAEAKESEEKPKEKNE 120

Query: 121 KSV 123
           KSV
Sbjct: 121 KSV 123

[Close]

BLAST of CSPI07G10300 vs. NCBI nr
Match: XP_038886854.1 (uncharacterized protein LOC120077074 [Benincasa hispida])

HSP 1 Score: 159.8 bits (403), Expect = 1.5e-35
Identity = 97/126 (76.98%), Postives = 106/126 (84.13%), Query Frame = 0


Query: 1   MGACATKPKVKADDLAAIPAPEPIDHDIVKNNEEVKFVEEEKKSDQTDEKKKENNEKVVV 60
           MGACATKPKVKADD+  IPAPEP+DHD+VKN EEV+ VEEEKK DQ DEKKKE+NEKVVV
Sbjct: 1   MGACATKPKVKADDMLVIPAPEPVDHDMVKNTEEVQVVEEEKKIDQNDEKKKEDNEKVVV 60

Query: 61  KEKKPPSLASLLVEKEAAPAVEVSGIPKAEEKPKA---KEVAAP-TIEAELKKSEEKPKE 120
           +E+KPPSLASLLVEKEAAP +EVS    AEEKPKA   KE  AP T  AE KKSEEKPKE
Sbjct: 61  QEEKPPSLASLLVEKEAAPVMEVSSETTAEEKPKAEPPKEAVAPTTAAAEEKKSEEKPKE 120

Query: 121 KDEKSV 123
           K+EKSV
Sbjct: 121 KEEKSV 126

[Close]

BLAST of CSPI07G10300 vs. NCBI nr
Match: KAA0041898.1 (major latex allergen Hev b 5-like [Cucumis melo var. makuwa] >TYK17832.1 major latex allergen Hev b 5-like [Cucumis melo var. makuwa])

HSP 1 Score: 118.6 bits (296), Expect = 3.7e-23
Identity = 63/74 (85.14%), Postives = 69/74 (93.24%), Query Frame = 0


Query: 1  MGACATKPKVKADDLAAIPAPEPIDHDIVKNNEEVKFVEEEKKSDQTDEKKKENNEKVVV 60
          MGACATKPKVK DDLA IPAPEPIDHDIVKNNEE+K VEEEKK DQTDEKKKE+NEKV+V
Sbjct: 1  MGACATKPKVKTDDLATIPAPEPIDHDIVKNNEEIKVVEEEKKVDQTDEKKKEDNEKVIV 60

Query: 61 KEKKPPSLASLLVE 75
          +++K PSLASLLVE
Sbjct: 61 EKEKSPSLASLLVE 74

The following BLAST results are available for this feature:

BLAST of CSPI07G10300 vs.


Match Name	E-value	Identity	Description

BLAST of CSPI07G10300 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K3E6	8.3e-53	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G230950 PE=4 SV=1	[more]
A0A1S3BGT9	3.6e-40	82.11	major latex allergen Hev b 5-like OS=Cucumis melo OX=3656 GN=LOC103489688 PE=4 S...	[more]
A0A5A7TGB5	1.8e-23	85.14	Major latex allergen Hev b 5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E567...	[more]
A0A6J1EUI1	1.4e-20	63.93	patellin-1-like OS=Cucurbita moschata OX=3662 GN=LOC111437877 PE=4 SV=1	[more]
A0A6J1DY67	4.5e-14	51.49	salt stress root protein RS1-like OS=Momordica charantia OX=3673 GN=LOC111024158...	[more]

BLAST of CSPI07G10300 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004139808.1	1.7e-52	100.00	microtubule-associated protein 1B isoform X1 [Cucumis sativus]	[more]
XP_031745011.1	1.2e-50	99.18	microtubule-associated protein 1B isoform X2 [Cucumis sativus] >KAE8646082.1 hyp...	[more]
XP_008447176.1	7.5e-40	82.11	PREDICTED: major latex allergen Hev b 5-like [Cucumis melo]	[more]
XP_038886854.1	1.5e-35	76.98	uncharacterized protein LOC120077074 [Benincasa hispida]	[more]
KAA0041898.1	3.7e-23	85.14	major latex allergen Hev b 5-like [Cucumis melo var. makuwa] >TYK17832.1 major l...	[more]

BLAST of CSPI07G10300 vs. NCBI nr


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 87..122
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 38..122
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 38..63

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI07G10300.1	CSPI07G10300.1	mRNA