CSPI07G08060 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI07G08060
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Descriptionallene oxide synthase 3-like
LocationChr7: 5726991 .. 5729548 (+)
RNA-Seq ExpressionCSPI07G08060
SyntenyCSPI07G08060
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTAAATTTTAGTGTATTTTTTGTAAGTGTTATCATAGATTGCATTATTACGTACCAATATAGTATACATATTTTAAATAAAAGAGTCGAACCAAAATATAAATTTGTCCTGTCACCATTCTCTTCCAACGTGAAGATAAGAAACCTTTTGTTTACTTTTTGTACGATCACAGTCACAGCAATGGCTTCTTCCTCCCCTGAACTTCCTCTCAAACCCATTCCCGGTGGCTATGGCTTCCCCTTCCTCGGTCCCATCAAAGACCGTTACGATTACTTCTATTTCCAAGGTAGAGACGAATTCTTCCGTTCCCGAATTACCAAATACAACTCCACCGTCTTCCACGCCAACATGCCACCGGGCCCCTTCATCTCCTCCGATTCCAGAGTCGTTGTCCTCCTCGATGCCCTCAGTTTTCCCATCCTCTTCGACACCACCAAAGTCGAGAAACGCAACATTCTCGACGGAACTTACATGCCCTCCTTGTCCTTCACCGGCGGCATTCGCACCTGTGCTTATTTGGACCCATCGGAAACAGAGCACACTGTTCTCAAACGCCTCTTTCTCTCCTTTCTCGCTTCTCGCCATGACAGGTTCATCCCTCTGTTTCGAAGCTCCTTGTCTGAGATGTTTGTTAAGCTTGAAGATAAACTCGCCGACAAAAATAAGATCGCTGATTTCAACTCGATTAGTGATGCCATGTCGTTTGATTATGTTTTCCGTTTATTCTCCGATGGAACCCCTGATTCGACATTAGCTGCTGATGGACCTGGAATGTTCGATTTATGGCTTGGGCTTCAACTTGCCCCATTGGCTTCCATTGGCCTTCCCAAAATTTTCTCTGTTTTTGAAGATCTCATTATTCACACCATTCCCCTGCCTTTCTTCCCAGTCAAGAGTCGTTACAGGTAGAGCGAGATTCAGAAACTTCTTTTAGATCCTGAAATGTTTGTGAATTTTTTAATTTCATTTGTTGAATTTGGTTTGACAGGAAGCTTTATAAAGCGTTTTACTCCTCCTCTGGCTCATTTCTAGACGAAGCAGAGAAACAGGGGATAGACAGAGAGAAAGCATGTCACAATTTAGTGTTTCTTGCTGGATTCAACGCATACGGGGGAATGAAAGTCCTTTTTCCCACTATACTGAAATGGGTCGGCACCGGTGGCGAGGATCTCCACCGTAAACTGGCGGAGGAAGTGAGGACAGCCGTGAAGGAAGAAGGGGGACTGACTTTCTCCGCCTTGGAGAAAATGAGTCTGCTGAAGTCGGTCGTGTACGAAGCTCTGAGGATCGAACCGCCGGTGCCGTTCCAGTACGGTAAAGCGAAGGAGGATATCGTGATTCAGAGCCACGATTCTTGTTTCAAGATCAAAAAAGGGGAGACGATTTTTGGTTATCAGCCGTTTGCTACTAAAGATCCGAAGATTTTTAAGGACTCGGAGAAGTTCGTGGGCGATAGGTTCGTGGGAGAAGAAGGGGAGAAGCTTTTGAAGTATGTTTACTGGTCAAATGAGCGGGAGACGGTGGAGCCGACGGCGGAGAACAAGCAGTGTCCGGGGAAGAATCTGGTGGTGATGATGGGCAGGATTATTGTGGTGGAATTCTTCCTCCGTTATGATACGTTCACTGTGGACGTCGCAGATTTGGCGCTGGGTCCGGCGGTGAAGTTCAAGTCCTTGACCAGAGCCACCGCTTCGGTTTAGAAGCTAATGACAAAATTAGTTTTTAATCATTTTACAGGTATTTTTAATTATGTATTTCAAAATTTACCTTTTTCTTTAAGGTCATACCAATTAGTGTATCAACTTTTATAATTTCTAAAGTAGTTTCCAAAAGCTTTTTTAATTTCTAAAGTAGTTTCCAAAAGCTTTTATAAATTCTAAAGTAGTTTCCAAAAGCTTTTATAATTTCTAAAGTAGTTTCCAAAAGTGTTACATACCACTTAATTATATTATTAGTATATTTGATGACAACTGAGATAGAATAGGTGTAGCATTTGTTAAATCGTCTATGCTTATATGTCTTTCATTTTTGTATATACAACTTTTAAAAGAAGAATGAGATGAGATTATTTTACTAAATAAACGACAAAATTGAAGATATTGTTGTAATTTATAATTCCATACTGAAGGCATCTGTTATTTTTATTTAGTTTATTTATGCGTCTAAATATATACAAAGAAATTGAAGGTTTTATTTTTTGAAGTTCAAAATTCAAATATATATATATATATATATATATAAATGTTTGTTGCTGATTGGCAGAATAAAGGAAGGGTGTTCGTGATTGGTTGATGCAGGGAAGCTGTGGTGGACTTGAGCACACATGACACATCATTGATTTGTTGGGGTTATAAAGTAATTTCCTCGTGATCCGCGTCGTTTTTAATTTGAGATCTCATTGTGTGTTGTAACCCGCGGGTGATCTTATTTTTATAGTTTCTTTTTTTTCAACTATGCTCCAATTTTTAAATTAAATAAATACCAAATATCAACTTCATAGTCTATAACCCTAAATTTTGAACTTTTATCAATTACAATTTAGGACTCATCTTGCCTCA

mRNA sequence

GTAAATTTTAGTGTATTTTTTGTAAGTGTTATCATAGATTGCATTATTACGTACCAATATAGTATACATATTTTAAATAAAAGAGTCGAACCAAAATATAAATTTGTCCTGTCACCATTCTCTTCCAACGTGAAGATAAGAAACCTTTTGTTTACTTTTTGTACGATCACAGTCACAGCAATGGCTTCTTCCTCCCCTGAACTTCCTCTCAAACCCATTCCCGGTGGCTATGGCTTCCCCTTCCTCGGTCCCATCAAAGACCGTTACGATTACTTCTATTTCCAAGGTAGAGACGAATTCTTCCGTTCCCGAATTACCAAATACAACTCCACCGTCTTCCACGCCAACATGCCACCGGGCCCCTTCATCTCCTCCGATTCCAGAGTCGTTGTCCTCCTCGATGCCCTCAGTTTTCCCATCCTCTTCGACACCACCAAAGTCGAGAAACGCAACATTCTCGACGGAACTTACATGCCCTCCTTGTCCTTCACCGGCGGCATTCGCACCTGTGCTTATTTGGACCCATCGGAAACAGAGCACACTGTTCTCAAACGCCTCTTTCTCTCCTTTCTCGCTTCTCGCCATGACAGGTTCATCCCTCTGTTTCGAAGCTCCTTGTCTGAGATGTTTGTTAAGCTTGAAGATAAACTCGCCGACAAAAATAAGATCGCTGATTTCAACTCGATTAGTGATGCCATGTCGTTTGATTATGTTTTCCGTTTATTCTCCGATGGAACCCCTGATTCGACATTAGCTGCTGATGGACCTGGAATGTTCGATTTATGGCTTGGGCTTCAACTTGCCCCATTGGCTTCCATTGGCCTTCCCAAAATTTTCTCTGTTTTTGAAGATCTCATTATTCACACCATTCCCCTGCCTTTCTTCCCAGTCAAGAGTCGTTACAGGAAGCTTTATAAAGCGTTTTACTCCTCCTCTGGCTCATTTCTAGACGAAGCAGAGAAACAGGGGATAGACAGAGAGAAAGCATGTCACAATTTAGTGTTTCTTGCTGGATTCAACGCATACGGGGGAATGAAAGTCCTTTTTCCCACTATACTGAAATGGGTCGGCACCGGTGGCGAGGATCTCCACCGTAAACTGGCGGAGGAAGTGAGGACAGCCGTGAAGGAAGAAGGGGGACTGACTTTCTCCGCCTTGGAGAAAATGAGTCTGCTGAAGTCGGTCGTGTACGAAGCTCTGAGGATCGAACCGCCGGTGCCGTTCCAGTACGGTAAAGCGAAGGAGGATATCGTGATTCAGAGCCACGATTCTTGTTTCAAGATCAAAAAAGGGGAGACGATTTTTGGTTATCAGCCGTTTGCTACTAAAGATCCGAAGATTTTTAAGGACTCGGAGAAGTTCGTGGGCGATAGGTTCGTGGGAGAAGAAGGGGAGAAGCTTTTGAAGTATGTTTACTGGTCAAATGAGCGGGAGACGGTGGAGCCGACGGCGGAGAACAAGCAGTGTCCGGGGAAGAATCTGGTGGTGATGATGGGCAGGATTATTGTGGTGGAATTCTTCCTCCGTTATGATACGTTCACTGTGGACGTCGCAGATTTGGCGCTGGGTCCGGCGGTGAAGTTCAAGTCCTTGACCAGAGCCACCGCTTCGGTTTAGAAGCTAATGACAAAATTAGTTTTTAATCATTTTACAGAATAAAGGAAGGGTGTTCGTGATTGGTTGATGCAGGGAAGCTGTGGTGGACTTGAGCACACATGACACATCATTGATTTGTTGGGGTTATAAAGTAATTTCCTCGTGATCCGCGTCGTTTTTAATTTGAGATCTCATTGTGTGTTGTAACCCGCGGGTGATCTTATTTTTATAGTTTCTTTTTTTTCAACTATGCTCCAATTTTTAAATTAAATAAATACCAAATATCAACTTCATAGTCTATAACCCTAAATTTTGAACTTTTATCAATTACAATTTAGGACTCATCTTGCCTCA

Coding sequence (CDS)

ATGGCTTCTTCCTCCCCTGAACTTCCTCTCAAACCCATTCCCGGTGGCTATGGCTTCCCCTTCCTCGGTCCCATCAAAGACCGTTACGATTACTTCTATTTCCAAGGTAGAGACGAATTCTTCCGTTCCCGAATTACCAAATACAACTCCACCGTCTTCCACGCCAACATGCCACCGGGCCCCTTCATCTCCTCCGATTCCAGAGTCGTTGTCCTCCTCGATGCCCTCAGTTTTCCCATCCTCTTCGACACCACCAAAGTCGAGAAACGCAACATTCTCGACGGAACTTACATGCCCTCCTTGTCCTTCACCGGCGGCATTCGCACCTGTGCTTATTTGGACCCATCGGAAACAGAGCACACTGTTCTCAAACGCCTCTTTCTCTCCTTTCTCGCTTCTCGCCATGACAGGTTCATCCCTCTGTTTCGAAGCTCCTTGTCTGAGATGTTTGTTAAGCTTGAAGATAAACTCGCCGACAAAAATAAGATCGCTGATTTCAACTCGATTAGTGATGCCATGTCGTTTGATTATGTTTTCCGTTTATTCTCCGATGGAACCCCTGATTCGACATTAGCTGCTGATGGACCTGGAATGTTCGATTTATGGCTTGGGCTTCAACTTGCCCCATTGGCTTCCATTGGCCTTCCCAAAATTTTCTCTGTTTTTGAAGATCTCATTATTCACACCATTCCCCTGCCTTTCTTCCCAGTCAAGAGTCGTTACAGGAAGCTTTATAAAGCGTTTTACTCCTCCTCTGGCTCATTTCTAGACGAAGCAGAGAAACAGGGGATAGACAGAGAGAAAGCATGTCACAATTTAGTGTTTCTTGCTGGATTCAACGCATACGGGGGAATGAAAGTCCTTTTTCCCACTATACTGAAATGGGTCGGCACCGGTGGCGAGGATCTCCACCGTAAACTGGCGGAGGAAGTGAGGACAGCCGTGAAGGAAGAAGGGGGACTGACTTTCTCCGCCTTGGAGAAAATGAGTCTGCTGAAGTCGGTCGTGTACGAAGCTCTGAGGATCGAACCGCCGGTGCCGTTCCAGTACGGTAAAGCGAAGGAGGATATCGTGATTCAGAGCCACGATTCTTGTTTCAAGATCAAAAAAGGGGAGACGATTTTTGGTTATCAGCCGTTTGCTACTAAAGATCCGAAGATTTTTAAGGACTCGGAGAAGTTCGTGGGCGATAGGTTCGTGGGAGAAGAAGGGGAGAAGCTTTTGAAGTATGTTTACTGGTCAAATGAGCGGGAGACGGTGGAGCCGACGGCGGAGAACAAGCAGTGTCCGGGGAAGAATCTGGTGGTGATGATGGGCAGGATTATTGTGGTGGAATTCTTCCTCCGTTATGATACGTTCACTGTGGACGTCGCAGATTTGGCGCTGGGTCCGGCGGTGAAGTTCAAGTCCTTGACCAGAGCCACCGCTTCGGTTTAG

Protein sequence

MASSSPELPLKPIPGGYGFPFLGPIKDRYDYFYFQGRDEFFRSRITKYNSTVFHANMPPGPFISSDSRVVVLLDALSFPILFDTTKVEKRNILDGTYMPSLSFTGGIRTCAYLDPSETEHTVLKRLFLSFLASRHDRFIPLFRSSLSEMFVKLEDKLADKNKIADFNSISDAMSFDYVFRLFSDGTPDSTLAADGPGMFDLWLGLQLAPLASIGLPKIFSVFEDLIIHTIPLPFFPVKSRYRKLYKAFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMKVLFPTILKWVGTGGEDLHRKLAEEVRTAVKEEGGLTFSALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIQSHDSCFKIKKGETIFGYQPFATKDPKIFKDSEKFVGDRFVGEEGEKLLKYVYWSNERETVEPTAENKQCPGKNLVVMMGRIIVVEFFLRYDTFTVDVADLALGPAVKFKSLTRATASV*
Homology
BLAST of CSPI07G08060 vs. ExPASy Swiss-Prot
Match: Q8GZP5 (Allene oxide synthase 3 OS=Solanum lycopersicum OX=4081 GN=AOS3 PE=1 SV=1)

HSP 1 Score: 571.2 bits (1471), Expect = 1.1e-161
Identity = 274/476 (57.56%), Postives = 363/476 (76.26%), Query Frame = 0

Query: 3   SSSPELPLKPIPGGYGFPFLGPIKDRYDYFYFQGRDEFFRSRITKYNSTVFHANMPPGPF 62
           SS P LP+K IPG YG PF G IKDRYD+ Y QG DEFFRSR+ KY+STVF  N+PPGPF
Sbjct: 18  SSIPSLPMKEIPGDYGVPFFGAIKDRYDFHYNQGADEFFRSRMKKYDSTVFRTNVPPGPF 77

Query: 63  ISSDSRVVVLLDALSFPILFDTTKVEKRNILDGTYMPSLSFTGGIRTCAYLDPSETEHTV 122
            + +S+VVVL+DA+S+PILFD ++V+K N  +GT+M S SF GG + C +L  S+ +HT 
Sbjct: 78  NARNSKVVVLVDAVSYPILFDNSQVDKENYFEGTFMSSPSFNGGYKVCGFLGTSDPKHTT 137

Query: 123 LKRLFLSFLASRHDRFIPLFRSSLSEMFVKLEDKLADKNKIADFNSISDAMSFDYVFRLF 182
           LK LFLS L   HD+FIP+F +S++ MF  LE +L++K   + FN I D +SF+++FRLF
Sbjct: 138 LKGLFLSTLTRLHDKFIPIFTTSITSMFTSLEKELSEKG-TSYFNPIGDNLSFEFLFRLF 197

Query: 183 SDGTP--DSTLAADGPGMFDLWLGLQLAPLASIGLPKIFSVFEDLIIHTIPLPFFPVKSR 242
            +G    D+++  +GP + D W+ LQLAPL S+GL  + +  EDL++HT PLP+  VK  
Sbjct: 198 CEGKNPIDTSVGPNGPKIVDKWVFLQLAPLISLGLKFVPNFLEDLVLHTFPLPYILVKRD 257

Query: 243 YRKLYKAFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMKVLFPTILKWVGTGG 302
           ++KLY AFY+S    LDEAEK G+ R++ACHN VFLAGFN+YGG+KV FP+++KW+GT G
Sbjct: 258 HQKLYNAFYNSMKDILDEAEKLGVKRDEACHNFVFLAGFNSYGGLKVFFPSLIKWIGTSG 317

Query: 303 EDLHRKLAEEVRTAVKEEGGLTFSALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIQ 362
             LH +L +E+RTAVKE GG+T SA++KM L+KSVVYE LR++PPVPFQ  KA+++I+I 
Sbjct: 318 PSLHARLVKEIRTAVKEAGGVTLSAIDKMPLVKSVVYETLRMDPPVPFQTVKARKNIIIT 377

Query: 363 SHDSCFKIKKGETIFGYQPFATKDPKIFKDSEKFVGDRFVGEEGEKLLKYVYWSNERETV 422
           +H+S F IKK E IFGYQP ATKD K+FK++E+F  DRFVG  GEKLLKYVYWSN +E  
Sbjct: 378 NHESSFLIKKDELIFGYQPLATKDSKVFKNAEEFNPDRFVG-GGEKLLKYVYWSNGKEID 437

Query: 423 EPTAENKQCPGKNLVVMMGRIIVVEFFLRYDTFTVDVADLALGPAVKFKSLTRATA 477
            P+  +KQCPGK+L+V+MGR++VVEFF+RYDTF V+   L LG  V FKSLT+AT+
Sbjct: 438 NPSVNDKQCPGKDLIVLMGRLLVVEFFMRYDTFEVEFGKLLLGSKVTFKSLTKATS 491

BLAST of CSPI07G08060 vs. ExPASy Swiss-Prot
Match: K4BV52 (Allene oxide synthase 1, chloroplastic OS=Solanum lycopersicum OX=4081 GN=AOS1 PE=2 SV=1)

HSP 1 Score: 518.8 bits (1335), Expect = 6.3e-146
Identity = 259/481 (53.85%), Postives = 341/481 (70.89%), Query Frame = 0

Query: 2   ASSSP-----ELPLKPIPGGYGFPFLGPIKDRYDYFYFQGRDEFFRSRITKYNSTVFHAN 61
           +SSSP     +LP + +PG YG P +GP KDR DYFY QG++EFF+SRI K+ STVF  N
Sbjct: 57  SSSSPPVKQAKLPAQKVPGDYGLPLVGPWKDRLDYFYNQGKNEFFKSRIQKHQSTVFRTN 116

Query: 62  MPPGPFISSDSRVVVLLDALSFPILFDTTKVEKRNILDGTYMPSLSFTGGIRTCAYLDPS 121
           MPPGPFIS +  VVVLLD  SFP+LFD +KVEK+++  GT+MPS   TGG R  +YLDPS
Sbjct: 117 MPPGPFISFNPNVVVLLDGKSFPVLFDVSKVEKKDLFTGTFMPSTDLTGGYRVLSYLDPS 176

Query: 122 ETEHTVLKRLFLSFLASRHDRFIPLFRSSLSEMFVKLEDKLADKNKIADFNSISDAMSFD 181
           E  H  LK+L    L+SR +  IP F +S SE+F  LE++L+ K K A  N+ +D  +F+
Sbjct: 177 EPNHAKLKKLMFYLLSSRRNEVIPEFHNSYSELFETLENELSTKGK-AGLNAANDQAAFN 236

Query: 182 YVFR-LFSDGTPDSTLAADGPGMFDLWLGLQLAPLASIGLPKIFSVFEDLIIHTIPLPFF 241
           ++ R L+     D+ L  DGP +   W+  QL PL  +GLPK   V EDL++HT  LP  
Sbjct: 237 FLARSLYGINPQDTELGTDGPKLIGKWVLFQLHPLLILGLPK---VLEDLVMHTFRLPPA 296

Query: 242 PVKSRYRKLYKAFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMKVLFPTILKW 301
            VK  Y++LY  FY +S S LDEAEK GI RE+ACHNL+F   FN++GG+K+ FP +LKW
Sbjct: 297 LVKKDYQRLYNFFYENSTSVLDEAEKIGISREEACHNLLFATCFNSFGGIKIFFPNMLKW 356

Query: 302 VGTGGEDLHRKLAEEVRTAVKEEGG-LTFSALEKMSLLKSVVYEALRIEPPVPFQYGKAK 361
           +G  G  LH +LA+E+R+ +    G +T +A+EKM L+KSVVYE+LRIEPPV  QYG+AK
Sbjct: 357 IGRAGAKLHSQLAQEIRSVISSNSGKVTMAAMEKMPLMKSVVYESLRIEPPVASQYGRAK 416

Query: 362 EDIVIQSHDSCFKIKKGETIFGYQPFATKDPKIFKDSEKFVGDRFVGEEGEKLLKYVYWS 421
            D+VI+SHD+ F+IK+GE ++GYQPFATKDPKIF  SE+FV DRF+GEEGEKLLK+V WS
Sbjct: 417 HDMVIESHDASFEIKEGELLYGYQPFATKDPKIFDRSEEFVADRFIGEEGEKLLKHVLWS 476

Query: 422 NERETVEPTAENKQCPGKNLVVMMGRIIVVEFFLRYDTFTVDVADLALGPAVKFKSLTRA 476
           N  ET   +  NKQC GK+ VV++ R+++VE FLRYD+F ++V    LG A+   SL RA
Sbjct: 477 NGSETENASINNKQCAGKDFVVLVSRLLLVELFLRYDSFEIEVGASPLGAAITLTSLRRA 533

BLAST of CSPI07G08060 vs. ExPASy Swiss-Prot
Match: Q7XYS3 (Allene oxide synthase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP74A2 PE=2 SV=2)

HSP 1 Score: 509.6 bits (1311), Expect = 3.8e-143
Identity = 251/474 (52.95%), Postives = 333/474 (70.25%), Query Frame = 0

Query: 8   LPLKPIPGGYGFPFLGPIKDRYDYFYFQGRDEFFRSRITKYNSTVFHANMPPGPFISSDS 67
           LP +P+PG YG PF+  ++DR D++Y QG+D++F SR  +Y STV   N+PPGPF++ D 
Sbjct: 7   LPRRPVPGSYGVPFVSAVRDRLDFYYLQGQDKYFESRAERYGSTVVRINVPPGPFMARDP 66

Query: 68  RVVVLLDALSFPILFDTTKVEKRNILDGTYMPSLSFTGGIRTCAYLDPSETEHTVLKRLF 127
           RVV LLDA SFP+LFD  KVEKR++  GT+MPS S TGG R CAYLDPSE  H  +K+L 
Sbjct: 67  RVVALLDAKSFPVLFDVAKVEKRDVFTGTFMPSTSLTGGYRVCAYLDPSEPNHAKIKQLL 126

Query: 128 LSFLASRHDRFIPLFRSSLSEMFVKLEDKLADKNKIADFNSISDAMSFDYVFRLFSDGTP 187
           LS L SR D F+P+FRS+   +   +E +LA     +DF +++DA SF+++   +    P
Sbjct: 127 LSLLVSRKDAFVPVFRSNFGALLDTVESQLASGGGKSDFTALNDATSFEFIGEAYFGVRP 186

Query: 188 --DSTLAADGPGMFDLWLGLQLAPLASIGLPKIFSVFEDLIIHTIPLPFFPVKSRYRKLY 247
              S+L   GP    LWL  QLAPL ++GLP I    ED ++HT+PLP F + S Y+ LY
Sbjct: 187 SASSSLGTGGPTKAALWLLWQLAPLTTLGLPMI---IEDPLLHTLPLPPFLISSDYKALY 246

Query: 248 KAFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMKVLFPTILKWVGTGGEDLHR 307
             F +++   LD AE  G+ RE+ACHNL+F   FN+YGG K+L P IL  V   GE LH 
Sbjct: 247 AYFAAAASQALDAAEGLGLSREEACHNLLFATVFNSYGGFKLLLPQILSRVAQAGEKLHE 306

Query: 308 KLAEEVRTAVKEEGG-LTFSALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIQSHDS 367
           +LA E+R+AV + GG +T +ALEKM L +SVV+EALR++PPV FQYG+AK D+ I+SHD+
Sbjct: 307 RLAAEIRSAVADAGGNVTLAALEKMELTRSVVWEALRLDPPVRFQYGRAKADLEIESHDA 366

Query: 368 CFKIKKGETIFGYQPFATKDPKIF-KDSEKFVGDRFVGEEGEKLLKYVYWSNERETVEPT 427
            F IKKGE +FGYQP AT+DP++F   + +FVGDRFVGEEG KLL+YVYWSN RET  P+
Sbjct: 367 SFAIKKGEMLFGYQPCATRDPRVFGATAREFVGDRFVGEEGRKLLQYVYWSNGRETENPS 426

Query: 428 AENKQCPGKNLVVMMGRIIVVEFFLRYDTFTVDVADLALGPAVKFKSLTRATAS 478
            +NKQCPGKNLVV++GR+++VE FLRYDTFT +      G  V    +T+A+ S
Sbjct: 427 VDNKQCPGKNLVVLVGRLLLVELFLRYDTFTAEA-----GKKVVITGVTKASTS 472

BLAST of CSPI07G08060 vs. ExPASy Swiss-Prot
Match: Q96242 (Allene oxide synthase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CYP74A PE=1 SV=3)

HSP 1 Score: 508.8 bits (1309), Expect = 6.5e-143
Identity = 253/475 (53.26%), Postives = 332/475 (69.89%), Query Frame = 0

Query: 3   SSSPELPLKPIPGGYGFPFLGPIKDRYDYFYFQGRDEFFRSRITKYNSTVFHANMPPGPF 62
           + S +LP++ IPG YG P +GPIKDR+DYFY QG +EFF+SRI KYNSTV+  NMPPG F
Sbjct: 47  TGSKDLPIRNIPGNYGLPIVGPIKDRWDYFYDQGAEEFFKSRIRKYNSTVYRVNMPPGAF 106

Query: 63  ISSDSRVVVLLDALSFPILFDTTKVEKRNILDGTYMPSLSFTGGIRTCAYLDPSETEHTV 122
           I+ + +VV LLD  SFP+LFD  KVEK+++  GTYMPS   TGG R  +YLDPSE +H  
Sbjct: 107 IAENPQVVALLDGKSFPVLFDVDKVEKKDLFTGTYMPSTELTGGYRILSYLDPSEPKHEK 166

Query: 123 LKRLFLSFLASRHDRFIPLFRSSLSEMFVKLEDKLADKNKIADFNSISDAMSFDYVFRLF 182
           LK L    L S  +R  P F+++ SE+F  LE +L+ K K ADF   SD  +F+++ R F
Sbjct: 167 LKNLLFFLLKSSRNRIFPEFQATYSELFDSLEKELSLKGK-ADFGGSSDGTAFNFLARAF 226

Query: 183 SDGTP-DSTLAADGPGMFDLWLGLQLAPLASIGLPKIFSVFEDLIIHTIPLPFFPVKSRY 242
               P D+ L AD PG+   W+   L PL SIGLP+   V E+ +IHT  LP   VKS Y
Sbjct: 227 YGTNPADTKLKADAPGLITKWVLFNLHPLLSIGLPR---VIEEPLIHTFSLPPALVKSDY 286

Query: 243 RKLYKAFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMKVLFPTILKWVGTGGE 302
           ++LY+ F  S+G  L EA+K GI RE+A HNL+F   FN +GGMK+LFP ++K +G  G 
Sbjct: 287 QRLYEFFLESAGEILVEADKLGISREEATHNLLFATCFNTWGGMKILFPNMVKRIGRAGH 346

Query: 303 DLHRKLAEEVRTAVKEEGG-LTFSALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIQ 362
            +H +LAEE+R+ +K  GG LT  A+EKM L KSVVYE LR EPPV  QYG+AK+D+VI+
Sbjct: 347 QVHNRLAEEIRSVIKSNGGELTMGAIEKMELTKSVVYECLRFEPPVTAQYGRAKKDLVIE 406

Query: 363 SHDSCFKIKKGETIFGYQPFATKDPKIFKDSEKFVGDRFVGEEGEKLLKYVYWSNERETV 422
           SHD+ FK+K GE ++GYQP AT+DPKIF  +++FV +RFVGEEGEKLL++V WSN  ET 
Sbjct: 407 SHDAAFKVKAGEMLYGYQPLATRDPKIFDRADEFVPERFVGEEGEKLLRHVLWSNGPETE 466

Query: 423 EPTAENKQCPGKNLVVMMGRIIVVEFFLRYDTFTVDVADLALGPAVKFKSLTRAT 476
            PT  NKQC GK+ VV++ R+ V+E F RYD+F ++V    LG +V F SL +A+
Sbjct: 467 TPTVGNKQCAGKDFVVLVARLFVIEIFRRYDSFDIEVGTSPLGSSVNFSSLRKAS 517

BLAST of CSPI07G08060 vs. ExPASy Swiss-Prot
Match: P48417 (Allene oxide synthase, chloroplastic OS=Linum usitatissimum OX=4006 GN=CYP74A PE=1 SV=1)

HSP 1 Score: 507.7 bits (1306), Expect = 1.5e-142
Identity = 247/477 (51.78%), Postives = 341/477 (71.49%), Query Frame = 0

Query: 2   ASSSPELPLKPIPGGYGFPFLGPIKDRYDYFYFQGRDEFFRSRITKYNSTVFHANMPPGP 61
           +S    LP++ IPG YG P +GPI+DR DYFY QGR+EFF+SR+ KY STV+ ANMPPGP
Sbjct: 64  SSDETTLPIRQIPGDYGLPGIGPIQDRLDYFYNQGREEFFKSRLQKYKSTVYRANMPPGP 123

Query: 62  FISSDSRVVVLLDALSFPILFDTTKVEKRNILDGTYMPSLSFTGGIRTCAYLDPSETEHT 121
           FI+S+ RV+VLLDA SFP+LFD +KVEK+++  GTYMPS   TGG R  +YLDPSE  HT
Sbjct: 124 FIASNPRVIVLLDAKSFPVLFDMSKVEKKDLFTGTYMPSTELTGGYRILSYLDPSEPNHT 183

Query: 122 VLKRLFLSFLASRHDRFIPLFRSSLSEMFVKLEDKLADKNKIADFNSISDAMSFDYVFRL 181
            LK+L  + + +R D  IP F SS +++   +E  LA K K A FN  ++  +F+++ R 
Sbjct: 184 KLKQLLFNLIKNRRDYVIPEFSSSFTDLCEVVEYDLATKGKAA-FNDPAEQAAFNFLSRA 243

Query: 182 FSDGTP-DSTLAADGPGMFDLWLGLQLAPLASIGLPKIFSVFEDLIIHTIPLPFFPVKSR 241
           F    P D+ L  D P +   W+   LAP+ S+GLPK     E+  +H++ LP   V++ 
Sbjct: 244 FFGVKPIDTPLGKDAPSLISKWVLFNLAPILSVGLPK---EVEEATLHSVRLPPLLVQND 303

Query: 242 YRKLYKAFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMKVLFPTILKWVGTGG 301
           Y +LY+ F S++GS LDEAE+ GI R++ACHN++F   FN++GG K+LFP+++KW+G  G
Sbjct: 304 YHRLYEFFTSAAGSVLDEAEQSGISRDEACHNILFAVCFNSWGGFKILFPSLMKWIGRAG 363

Query: 302 EDLHRKLAEEVRTAVKEEGG--LTFSALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIV 361
            +LH KLA+E+R+A++  GG  +T +A+E+M L+KSVVYE LRIEPPV  QYGKAK+D +
Sbjct: 364 LELHTKLAQEIRSAIQSTGGGKVTMAAMEQMPLMKSVVYETLRIEPPVALQYGKAKKDFI 423

Query: 362 IQSHDSCFKIKKGETIFGYQPFATKDPKIFKDSEKFVGDRFVGEEGEKLLKYVYWSNERE 421
           ++SH++ +++K+GE +FGYQPFATKDPKIF   E+FV DRFVG EG KL++YV WSN  E
Sbjct: 424 LESHEAAYQVKEGEMLFGYQPFATKDPKIFDRPEEFVADRFVG-EGVKLMEYVMWSNGPE 483

Query: 422 TVEPTAENKQCPGKNLVVMMGRIIVVEFFLRYDTFTVDVADLALGPAVKFKSLTRAT 476
           T  P+  NKQC GK+ VVM  R+ VVE F RYD+F ++V   +LG ++   SL R+T
Sbjct: 484 TETPSVANKQCAGKDFVVMAARLFVVELFKRYDSFDIEVGTSSLGASITLTSLKRST 535

BLAST of CSPI07G08060 vs. ExPASy TrEMBL
Match: U5YTM6 (Hydroperoxide lyase OS=Cucumis sativus OX=3659 GN=HPL PE=2 SV=1)

HSP 1 Score: 960.3 bits (2481), Expect = 3.0e-276
Identity = 477/478 (99.79%), Postives = 477/478 (99.79%), Query Frame = 0

Query: 1   MASSSPELPLKPIPGGYGFPFLGPIKDRYDYFYFQGRDEFFRSRITKYNSTVFHANMPPG 60
           MASSSPELPLKPIPGGYGFPFLGPIKDRYDYFYFQGRDEFFRSRITKYNSTVFHANMPPG
Sbjct: 1   MASSSPELPLKPIPGGYGFPFLGPIKDRYDYFYFQGRDEFFRSRITKYNSTVFHANMPPG 60

Query: 61  PFISSDSRVVVLLDALSFPILFDTTKVEKRNILDGTYMPSLSFTGGIRTCAYLDPSETEH 120
           PFISSDSRVVVLLDALSFPILFDTTKVEKRNILDGTYMPSLSFTGGIRTCAYLDPSETEH
Sbjct: 61  PFISSDSRVVVLLDALSFPILFDTTKVEKRNILDGTYMPSLSFTGGIRTCAYLDPSETEH 120

Query: 121 TVLKRLFLSFLASRHDRFIPLFRSSLSEMFVKLEDKLADKNKIADFNSISDAMSFDYVFR 180
           TVLKRLFLSFLASRHDRFIPLFRSSLSEMFVKLEDKLADKNKIADFNSISDAMSFDYVFR
Sbjct: 121 TVLKRLFLSFLASRHDRFIPLFRSSLSEMFVKLEDKLADKNKIADFNSISDAMSFDYVFR 180

Query: 181 LFSDGTPDSTLAADGPGMFDLWLGLQLAPLASIGLPKIFSVFEDLIIHTIPLPFFPVKSR 240
           LFSDGTPDSTLAADGPGMFDLWLGLQLAPLASIGLPKIFSVFEDLIIHTIPLPFFPVKSR
Sbjct: 181 LFSDGTPDSTLAADGPGMFDLWLGLQLAPLASIGLPKIFSVFEDLIIHTIPLPFFPVKSR 240

Query: 241 YRKLYKAFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMKVLFPTILKWVGTGG 300
           YRKLYKAFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMKVLFPTILKWVGTGG
Sbjct: 241 YRKLYKAFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMKVLFPTILKWVGTGG 300

Query: 301 EDLHRKLAEEVRTAVKEEGGLTFSALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIQ 360
           EDLHRKLAEEVRT VKEEGGLTFSALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIQ
Sbjct: 301 EDLHRKLAEEVRTTVKEEGGLTFSALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIQ 360

Query: 361 SHDSCFKIKKGETIFGYQPFATKDPKIFKDSEKFVGDRFVGEEGEKLLKYVYWSNERETV 420
           SHDSCFKIKKGETIFGYQPFATKDPKIFKDSEKFVGDRFVGEEGEKLLKYVYWSNERETV
Sbjct: 361 SHDSCFKIKKGETIFGYQPFATKDPKIFKDSEKFVGDRFVGEEGEKLLKYVYWSNERETV 420

Query: 421 EPTAENKQCPGKNLVVMMGRIIVVEFFLRYDTFTVDVADLALGPAVKFKSLTRATASV 479
           EPTAENKQCPGKNLVVMMGRIIVVEFFLRYDTFTVDVADLALGPAVKFKSLTRATASV
Sbjct: 421 EPTAENKQCPGKNLVVMMGRIIVVEFFLRYDTFTVDVADLALGPAVKFKSLTRATASV 478

BLAST of CSPI07G08060 vs. ExPASy TrEMBL
Match: Q9M5J2 (Fatty acid hydroperoxide lyase OS=Cucumis sativus OX=3659 PE=2 SV=1)

HSP 1 Score: 956.8 bits (2472), Expect = 3.3e-275
Identity = 475/478 (99.37%), Postives = 476/478 (99.58%), Query Frame = 0

Query: 1   MASSSPELPLKPIPGGYGFPFLGPIKDRYDYFYFQGRDEFFRSRITKYNSTVFHANMPPG 60
           MASSSPELPLKPIPGGYGFPFLGPIKDRYDYFYFQGRDEFFRSRITKYNSTVFHANMPPG
Sbjct: 1   MASSSPELPLKPIPGGYGFPFLGPIKDRYDYFYFQGRDEFFRSRITKYNSTVFHANMPPG 60

Query: 61  PFISSDSRVVVLLDALSFPILFDTTKVEKRNILDGTYMPSLSFTGGIRTCAYLDPSETEH 120
           PFISSDSRVVVLLDALSFPILFDTTKVEKRNILDGTYMPSLSFTGGIRTCAYLDPSETEH
Sbjct: 61  PFISSDSRVVVLLDALSFPILFDTTKVEKRNILDGTYMPSLSFTGGIRTCAYLDPSETEH 120

Query: 121 TVLKRLFLSFLASRHDRFIPLFRSSLSEMFVKLEDKLADKNKIADFNSISDAMSFDYVFR 180
           TVLKRLFLSFLAS HDRFIPLFRSSLSEMFVKLEDKLADKNKIADFNSISDA+SFDYVFR
Sbjct: 121 TVLKRLFLSFLASHHDRFIPLFRSSLSEMFVKLEDKLADKNKIADFNSISDAVSFDYVFR 180

Query: 181 LFSDGTPDSTLAADGPGMFDLWLGLQLAPLASIGLPKIFSVFEDLIIHTIPLPFFPVKSR 240
           LFSDGTPDSTLAADGPGMFDLWLGLQLAPLASIGLPKIFSVFEDLIIHTIPLPFFPVKSR
Sbjct: 181 LFSDGTPDSTLAADGPGMFDLWLGLQLAPLASIGLPKIFSVFEDLIIHTIPLPFFPVKSR 240

Query: 241 YRKLYKAFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMKVLFPTILKWVGTGG 300
           YRKLYKAFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMKVLFPTILKWVGTGG
Sbjct: 241 YRKLYKAFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMKVLFPTILKWVGTGG 300

Query: 301 EDLHRKLAEEVRTAVKEEGGLTFSALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIQ 360
           EDLHRKLAEEVRT VKEEGGLTFSALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIQ
Sbjct: 301 EDLHRKLAEEVRTTVKEEGGLTFSALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIQ 360

Query: 361 SHDSCFKIKKGETIFGYQPFATKDPKIFKDSEKFVGDRFVGEEGEKLLKYVYWSNERETV 420
           SHDSCFKIKKGETIFGYQPFATKDPKIFKDSEKFVGDRFVGEEGEKLLKYVYWSNERETV
Sbjct: 361 SHDSCFKIKKGETIFGYQPFATKDPKIFKDSEKFVGDRFVGEEGEKLLKYVYWSNERETV 420

Query: 421 EPTAENKQCPGKNLVVMMGRIIVVEFFLRYDTFTVDVADLALGPAVKFKSLTRATASV 479
           EPTAENKQCPGKNLVVMMGRIIVVEFFLRYDTFTVDVADLALGPAVKFKSLTRATASV
Sbjct: 421 EPTAENKQCPGKNLVVMMGRIIVVEFFLRYDTFTVDVADLALGPAVKFKSLTRATASV 478

BLAST of CSPI07G08060 vs. ExPASy TrEMBL
Match: A0A5D3C5U2 (Allene oxide synthase 2-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G00110 PE=4 SV=1)

HSP 1 Score: 907.5 bits (2344), Expect = 2.3e-260
Identity = 449/477 (94.13%), Postives = 462/477 (96.86%), Query Frame = 0

Query: 2   ASSSPELPLKPIPGGYGFPFLGPIKDRYDYFYFQGRDEFFRSRITKYNSTVFHANMPPGP 61
           +SSSPELPLKPIPGGYGFPFLGPIKDRYDYFYFQGRDEFFRSRITKYNSTVF ANMPPGP
Sbjct: 5   SSSSPELPLKPIPGGYGFPFLGPIKDRYDYFYFQGRDEFFRSRITKYNSTVFRANMPPGP 64

Query: 62  FISSDSRVVVLLDALSFPILFDTTKVEKRNILDGTYMPSLSFTGGIRTCAYLDPSETEHT 121
           FISSDSRVVVLLDALSFPILFDT KVEKRNILDGTYMPSLSFTG IRTCAYLDPSETEH+
Sbjct: 65  FISSDSRVVVLLDALSFPILFDTAKVEKRNILDGTYMPSLSFTGNIRTCAYLDPSETEHS 124

Query: 122 VLKRLFLSFLASRHDRFIPLFRSSLSEMFVKLEDKLADKNKIADFNSISDAMSFDYVFRL 181
           VLKRLFLSFLASRHDRFIPLFRSSLSEMFVKLEDKL++K KIADFNSISD+MSFDYVFRL
Sbjct: 125 VLKRLFLSFLASRHDRFIPLFRSSLSEMFVKLEDKLSEKKKIADFNSISDSMSFDYVFRL 184

Query: 182 FSDGTPDSTLAADGPGMFDLWLGLQLAPLASIGLPKIFSVFEDLIIHTIPLPFFPVKSRY 241
            SDGTPDS LAA+GPGMFDLWL  QLAPLASIGLPKIFSVFEDL+IHTIPLPFFPVKS Y
Sbjct: 185 LSDGTPDSKLAAEGPGMFDLWLVFQLAPLASIGLPKIFSVFEDLVIHTIPLPFFPVKSGY 244

Query: 242 RKLYKAFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMKVLFPTILKWVGTGGE 301
           RKLY+AFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMKVLFPT+LKWVGT GE
Sbjct: 245 RKLYEAFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMKVLFPTLLKWVGTAGE 304

Query: 302 DLHRKLAEEVRTAVKEEGGLTFSALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIQS 361
           DLHRKLAEEVRT VKEEGGLTFSALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIQS
Sbjct: 305 DLHRKLAEEVRTTVKEEGGLTFSALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIQS 364

Query: 362 HDSCFKIKKGETIFGYQPFATKDPKIFKDSEKFVGDRFVGEEGEKLLKYVYWSNERETVE 421
           HDS FKIKKGETIFGYQPFATKDPKIFKDSEKFVGDRFVGEEGEKLLKYVYWSNERETVE
Sbjct: 365 HDSSFKIKKGETIFGYQPFATKDPKIFKDSEKFVGDRFVGEEGEKLLKYVYWSNERETVE 424

Query: 422 PTAENKQCPGKNLVVMMGRIIVVEFFLRYDTFTVDVADLALGPAVKFKSLTRATASV 479
           PTAENKQCPGKNLVV++GRI+VVEFFLRYDTFTV+VADL LGPAVKFKSLTRAT  V
Sbjct: 425 PTAENKQCPGKNLVVLIGRIMVVEFFLRYDTFTVEVADLPLGPAVKFKSLTRATDMV 481

BLAST of CSPI07G08060 vs. ExPASy TrEMBL
Match: A0A1S3BZK9 (allene oxide synthase 2-like isoform X1 OS=Cucumis melo OX=3656 GN=HPL PE=4 SV=1)

HSP 1 Score: 907.5 bits (2344), Expect = 2.3e-260
Identity = 449/477 (94.13%), Postives = 462/477 (96.86%), Query Frame = 0

Query: 2   ASSSPELPLKPIPGGYGFPFLGPIKDRYDYFYFQGRDEFFRSRITKYNSTVFHANMPPGP 61
           +SSSPELPLKPIPGGYGFPFLGPIKDRYDYFYFQGRDEFFRSRITKYNSTVF ANMPPGP
Sbjct: 5   SSSSPELPLKPIPGGYGFPFLGPIKDRYDYFYFQGRDEFFRSRITKYNSTVFRANMPPGP 64

Query: 62  FISSDSRVVVLLDALSFPILFDTTKVEKRNILDGTYMPSLSFTGGIRTCAYLDPSETEHT 121
           FISSDSRVVVLLDALSFPILFDT KVEKRNILDGTYMPSLSFTG IRTCAYLDPSETEH+
Sbjct: 65  FISSDSRVVVLLDALSFPILFDTAKVEKRNILDGTYMPSLSFTGNIRTCAYLDPSETEHS 124

Query: 122 VLKRLFLSFLASRHDRFIPLFRSSLSEMFVKLEDKLADKNKIADFNSISDAMSFDYVFRL 181
           VLKRLFLSFLASRHDRFIPLFRSSLSEMFVKLEDKL++K KIADFNSISD+MSFDYVFRL
Sbjct: 125 VLKRLFLSFLASRHDRFIPLFRSSLSEMFVKLEDKLSEKKKIADFNSISDSMSFDYVFRL 184

Query: 182 FSDGTPDSTLAADGPGMFDLWLGLQLAPLASIGLPKIFSVFEDLIIHTIPLPFFPVKSRY 241
            SDGTPDS LAA+GPGMFDLWL  QLAPLASIGLPKIFSVFEDL+IHTIPLPFFPVKS Y
Sbjct: 185 LSDGTPDSKLAAEGPGMFDLWLVFQLAPLASIGLPKIFSVFEDLVIHTIPLPFFPVKSGY 244

Query: 242 RKLYKAFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMKVLFPTILKWVGTGGE 301
           RKLY+AFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMKVLFPT+LKWVGT GE
Sbjct: 245 RKLYEAFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMKVLFPTLLKWVGTAGE 304

Query: 302 DLHRKLAEEVRTAVKEEGGLTFSALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIQS 361
           DLHRKLAEEVRT VKEEGGLTFSALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIQS
Sbjct: 305 DLHRKLAEEVRTTVKEEGGLTFSALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIQS 364

Query: 362 HDSCFKIKKGETIFGYQPFATKDPKIFKDSEKFVGDRFVGEEGEKLLKYVYWSNERETVE 421
           HDS FKIKKGETIFGYQPFATKDPKIFKDSEKFVGDRFVGEEGEKLLKYVYWSNERETVE
Sbjct: 365 HDSSFKIKKGETIFGYQPFATKDPKIFKDSEKFVGDRFVGEEGEKLLKYVYWSNERETVE 424

Query: 422 PTAENKQCPGKNLVVMMGRIIVVEFFLRYDTFTVDVADLALGPAVKFKSLTRATASV 479
           PTAENKQCPGKNLVV++GRI+VVEFFLRYDTFTV+VADL LGPAVKFKSLTRAT  V
Sbjct: 425 PTAENKQCPGKNLVVLIGRIMVVEFFLRYDTFTVEVADLPLGPAVKFKSLTRATDMV 481

BLAST of CSPI07G08060 vs. ExPASy TrEMBL
Match: Q93XR3 (Fatty acid 9-hydroperoxide lyase OS=Cucumis melo OX=3656 GN=HPL PE=2 SV=1)

HSP 1 Score: 905.6 bits (2339), Expect = 8.9e-260
Identity = 448/477 (93.92%), Postives = 461/477 (96.65%), Query Frame = 0

Query: 2   ASSSPELPLKPIPGGYGFPFLGPIKDRYDYFYFQGRDEFFRSRITKYNSTVFHANMPPGP 61
           +SSSPELPLKPIPGGYGFPFLGPIKDRYDYFYFQGRDEFFRSRITKYNSTVF ANMPPGP
Sbjct: 5   SSSSPELPLKPIPGGYGFPFLGPIKDRYDYFYFQGRDEFFRSRITKYNSTVFRANMPPGP 64

Query: 62  FISSDSRVVVLLDALSFPILFDTTKVEKRNILDGTYMPSLSFTGGIRTCAYLDPSETEHT 121
           FISSDSRVVVLLDALSFPILFDT KVEKRNILDGTYMPSLSFTG IRTCAYLDPSETEH+
Sbjct: 65  FISSDSRVVVLLDALSFPILFDTAKVEKRNILDGTYMPSLSFTGNIRTCAYLDPSETEHS 124

Query: 122 VLKRLFLSFLASRHDRFIPLFRSSLSEMFVKLEDKLADKNKIADFNSISDAMSFDYVFRL 181
           VLKRLFLSFLASRHDRFIPLFRSSLSEMFVKLEDKL++K KIADFNSISD+MSFDYVFRL
Sbjct: 125 VLKRLFLSFLASRHDRFIPLFRSSLSEMFVKLEDKLSEKKKIADFNSISDSMSFDYVFRL 184

Query: 182 FSDGTPDSTLAADGPGMFDLWLGLQLAPLASIGLPKIFSVFEDLIIHTIPLPFFPVKSRY 241
            SDGTPDS LAA+GPGMFDLWL  QLAPLASIGLPKIFSVFEDL+IHTIPLPFFPVKS Y
Sbjct: 185 LSDGTPDSKLAAEGPGMFDLWLVFQLAPLASIGLPKIFSVFEDLVIHTIPLPFFPVKSGY 244

Query: 242 RKLYKAFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMKVLFPTILKWVGTGGE 301
           RKLY+AFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMKVLFPT+LKWVGT GE
Sbjct: 245 RKLYEAFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMKVLFPTLLKWVGTAGE 304

Query: 302 DLHRKLAEEVRTAVKEEGGLTFSALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIQS 361
           DLHRKLAEEVRT VKEEGGLTFSALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIQS
Sbjct: 305 DLHRKLAEEVRTTVKEEGGLTFSALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIQS 364

Query: 362 HDSCFKIKKGETIFGYQPFATKDPKIFKDSEKFVGDRFVGEEGEKLLKYVYWSNERETVE 421
           HDS FKIKKGETIFGYQPFATKDPKIFKDSEKFVGDRFVGEEGEKLLKYVYWSNERETVE
Sbjct: 365 HDSSFKIKKGETIFGYQPFATKDPKIFKDSEKFVGDRFVGEEGEKLLKYVYWSNERETVE 424

Query: 422 PTAENKQCPGKNLVVMMGRIIVVEFFLRYDTFTVDVADLALGPAVKFKSLTRATASV 479
           PT ENKQCPGKNLVV++GRI+VVEFFLRYDTFTV+VADL LGPAVKFKSLTRAT  V
Sbjct: 425 PTPENKQCPGKNLVVLIGRIMVVEFFLRYDTFTVEVADLPLGPAVKFKSLTRATDMV 481

BLAST of CSPI07G08060 vs. NCBI nr
Match: NP_001274399.1 (allene oxide synthase, chloroplastic-like [Cucumis sativus] >XP_011658536.1 allene oxide synthase, chloroplastic-like isoform X1 [Cucumis sativus] >XP_031744184.1 allene oxide synthase, chloroplastic-like isoform X1 [Cucumis sativus] >AGZ95025.1 hydroperoxide lyase [Cucumis sativus] >AHC08715.1 hydroperoxide lyase [Cucumis sativus] >KGN43979.1 hypothetical protein Csa_017689 [Cucumis sativus])

HSP 1 Score: 960.3 bits (2481), Expect = 6.3e-276
Identity = 477/478 (99.79%), Postives = 477/478 (99.79%), Query Frame = 0

Query: 1   MASSSPELPLKPIPGGYGFPFLGPIKDRYDYFYFQGRDEFFRSRITKYNSTVFHANMPPG 60
           MASSSPELPLKPIPGGYGFPFLGPIKDRYDYFYFQGRDEFFRSRITKYNSTVFHANMPPG
Sbjct: 1   MASSSPELPLKPIPGGYGFPFLGPIKDRYDYFYFQGRDEFFRSRITKYNSTVFHANMPPG 60

Query: 61  PFISSDSRVVVLLDALSFPILFDTTKVEKRNILDGTYMPSLSFTGGIRTCAYLDPSETEH 120
           PFISSDSRVVVLLDALSFPILFDTTKVEKRNILDGTYMPSLSFTGGIRTCAYLDPSETEH
Sbjct: 61  PFISSDSRVVVLLDALSFPILFDTTKVEKRNILDGTYMPSLSFTGGIRTCAYLDPSETEH 120

Query: 121 TVLKRLFLSFLASRHDRFIPLFRSSLSEMFVKLEDKLADKNKIADFNSISDAMSFDYVFR 180
           TVLKRLFLSFLASRHDRFIPLFRSSLSEMFVKLEDKLADKNKIADFNSISDAMSFDYVFR
Sbjct: 121 TVLKRLFLSFLASRHDRFIPLFRSSLSEMFVKLEDKLADKNKIADFNSISDAMSFDYVFR 180

Query: 181 LFSDGTPDSTLAADGPGMFDLWLGLQLAPLASIGLPKIFSVFEDLIIHTIPLPFFPVKSR 240
           LFSDGTPDSTLAADGPGMFDLWLGLQLAPLASIGLPKIFSVFEDLIIHTIPLPFFPVKSR
Sbjct: 181 LFSDGTPDSTLAADGPGMFDLWLGLQLAPLASIGLPKIFSVFEDLIIHTIPLPFFPVKSR 240

Query: 241 YRKLYKAFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMKVLFPTILKWVGTGG 300
           YRKLYKAFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMKVLFPTILKWVGTGG
Sbjct: 241 YRKLYKAFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMKVLFPTILKWVGTGG 300

Query: 301 EDLHRKLAEEVRTAVKEEGGLTFSALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIQ 360
           EDLHRKLAEEVRT VKEEGGLTFSALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIQ
Sbjct: 301 EDLHRKLAEEVRTTVKEEGGLTFSALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIQ 360

Query: 361 SHDSCFKIKKGETIFGYQPFATKDPKIFKDSEKFVGDRFVGEEGEKLLKYVYWSNERETV 420
           SHDSCFKIKKGETIFGYQPFATKDPKIFKDSEKFVGDRFVGEEGEKLLKYVYWSNERETV
Sbjct: 361 SHDSCFKIKKGETIFGYQPFATKDPKIFKDSEKFVGDRFVGEEGEKLLKYVYWSNERETV 420

Query: 421 EPTAENKQCPGKNLVVMMGRIIVVEFFLRYDTFTVDVADLALGPAVKFKSLTRATASV 479
           EPTAENKQCPGKNLVVMMGRIIVVEFFLRYDTFTVDVADLALGPAVKFKSLTRATASV
Sbjct: 421 EPTAENKQCPGKNLVVMMGRIIVVEFFLRYDTFTVDVADLALGPAVKFKSLTRATASV 478

BLAST of CSPI07G08060 vs. NCBI nr
Match: AAF64041.1 (fatty acid hydroperoxide lyase [Cucumis sativus])

HSP 1 Score: 956.8 bits (2472), Expect = 6.9e-275
Identity = 475/478 (99.37%), Postives = 476/478 (99.58%), Query Frame = 0

Query: 1   MASSSPELPLKPIPGGYGFPFLGPIKDRYDYFYFQGRDEFFRSRITKYNSTVFHANMPPG 60
           MASSSPELPLKPIPGGYGFPFLGPIKDRYDYFYFQGRDEFFRSRITKYNSTVFHANMPPG
Sbjct: 1   MASSSPELPLKPIPGGYGFPFLGPIKDRYDYFYFQGRDEFFRSRITKYNSTVFHANMPPG 60

Query: 61  PFISSDSRVVVLLDALSFPILFDTTKVEKRNILDGTYMPSLSFTGGIRTCAYLDPSETEH 120
           PFISSDSRVVVLLDALSFPILFDTTKVEKRNILDGTYMPSLSFTGGIRTCAYLDPSETEH
Sbjct: 61  PFISSDSRVVVLLDALSFPILFDTTKVEKRNILDGTYMPSLSFTGGIRTCAYLDPSETEH 120

Query: 121 TVLKRLFLSFLASRHDRFIPLFRSSLSEMFVKLEDKLADKNKIADFNSISDAMSFDYVFR 180
           TVLKRLFLSFLAS HDRFIPLFRSSLSEMFVKLEDKLADKNKIADFNSISDA+SFDYVFR
Sbjct: 121 TVLKRLFLSFLASHHDRFIPLFRSSLSEMFVKLEDKLADKNKIADFNSISDAVSFDYVFR 180

Query: 181 LFSDGTPDSTLAADGPGMFDLWLGLQLAPLASIGLPKIFSVFEDLIIHTIPLPFFPVKSR 240
           LFSDGTPDSTLAADGPGMFDLWLGLQLAPLASIGLPKIFSVFEDLIIHTIPLPFFPVKSR
Sbjct: 181 LFSDGTPDSTLAADGPGMFDLWLGLQLAPLASIGLPKIFSVFEDLIIHTIPLPFFPVKSR 240

Query: 241 YRKLYKAFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMKVLFPTILKWVGTGG 300
           YRKLYKAFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMKVLFPTILKWVGTGG
Sbjct: 241 YRKLYKAFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMKVLFPTILKWVGTGG 300

Query: 301 EDLHRKLAEEVRTAVKEEGGLTFSALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIQ 360
           EDLHRKLAEEVRT VKEEGGLTFSALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIQ
Sbjct: 301 EDLHRKLAEEVRTTVKEEGGLTFSALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIQ 360

Query: 361 SHDSCFKIKKGETIFGYQPFATKDPKIFKDSEKFVGDRFVGEEGEKLLKYVYWSNERETV 420
           SHDSCFKIKKGETIFGYQPFATKDPKIFKDSEKFVGDRFVGEEGEKLLKYVYWSNERETV
Sbjct: 361 SHDSCFKIKKGETIFGYQPFATKDPKIFKDSEKFVGDRFVGEEGEKLLKYVYWSNERETV 420

Query: 421 EPTAENKQCPGKNLVVMMGRIIVVEFFLRYDTFTVDVADLALGPAVKFKSLTRATASV 479
           EPTAENKQCPGKNLVVMMGRIIVVEFFLRYDTFTVDVADLALGPAVKFKSLTRATASV
Sbjct: 421 EPTAENKQCPGKNLVVMMGRIIVVEFFLRYDTFTVDVADLALGPAVKFKSLTRATASV 478

BLAST of CSPI07G08060 vs. NCBI nr
Match: XP_008454885.1 (PREDICTED: allene oxide synthase 2-like isoform X1 [Cucumis melo] >KAA0031259.1 allene oxide synthase 2-like isoform X1 [Cucumis melo var. makuwa] >TYK06710.1 allene oxide synthase 2-like isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 907.5 bits (2344), Expect = 4.8e-260
Identity = 449/477 (94.13%), Postives = 462/477 (96.86%), Query Frame = 0

Query: 2   ASSSPELPLKPIPGGYGFPFLGPIKDRYDYFYFQGRDEFFRSRITKYNSTVFHANMPPGP 61
           +SSSPELPLKPIPGGYGFPFLGPIKDRYDYFYFQGRDEFFRSRITKYNSTVF ANMPPGP
Sbjct: 5   SSSSPELPLKPIPGGYGFPFLGPIKDRYDYFYFQGRDEFFRSRITKYNSTVFRANMPPGP 64

Query: 62  FISSDSRVVVLLDALSFPILFDTTKVEKRNILDGTYMPSLSFTGGIRTCAYLDPSETEHT 121
           FISSDSRVVVLLDALSFPILFDT KVEKRNILDGTYMPSLSFTG IRTCAYLDPSETEH+
Sbjct: 65  FISSDSRVVVLLDALSFPILFDTAKVEKRNILDGTYMPSLSFTGNIRTCAYLDPSETEHS 124

Query: 122 VLKRLFLSFLASRHDRFIPLFRSSLSEMFVKLEDKLADKNKIADFNSISDAMSFDYVFRL 181
           VLKRLFLSFLASRHDRFIPLFRSSLSEMFVKLEDKL++K KIADFNSISD+MSFDYVFRL
Sbjct: 125 VLKRLFLSFLASRHDRFIPLFRSSLSEMFVKLEDKLSEKKKIADFNSISDSMSFDYVFRL 184

Query: 182 FSDGTPDSTLAADGPGMFDLWLGLQLAPLASIGLPKIFSVFEDLIIHTIPLPFFPVKSRY 241
            SDGTPDS LAA+GPGMFDLWL  QLAPLASIGLPKIFSVFEDL+IHTIPLPFFPVKS Y
Sbjct: 185 LSDGTPDSKLAAEGPGMFDLWLVFQLAPLASIGLPKIFSVFEDLVIHTIPLPFFPVKSGY 244

Query: 242 RKLYKAFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMKVLFPTILKWVGTGGE 301
           RKLY+AFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMKVLFPT+LKWVGT GE
Sbjct: 245 RKLYEAFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMKVLFPTLLKWVGTAGE 304

Query: 302 DLHRKLAEEVRTAVKEEGGLTFSALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIQS 361
           DLHRKLAEEVRT VKEEGGLTFSALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIQS
Sbjct: 305 DLHRKLAEEVRTTVKEEGGLTFSALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIQS 364

Query: 362 HDSCFKIKKGETIFGYQPFATKDPKIFKDSEKFVGDRFVGEEGEKLLKYVYWSNERETVE 421
           HDS FKIKKGETIFGYQPFATKDPKIFKDSEKFVGDRFVGEEGEKLLKYVYWSNERETVE
Sbjct: 365 HDSSFKIKKGETIFGYQPFATKDPKIFKDSEKFVGDRFVGEEGEKLLKYVYWSNERETVE 424

Query: 422 PTAENKQCPGKNLVVMMGRIIVVEFFLRYDTFTVDVADLALGPAVKFKSLTRATASV 479
           PTAENKQCPGKNLVV++GRI+VVEFFLRYDTFTV+VADL LGPAVKFKSLTRAT  V
Sbjct: 425 PTAENKQCPGKNLVVLIGRIMVVEFFLRYDTFTVEVADLPLGPAVKFKSLTRATDMV 481

BLAST of CSPI07G08060 vs. NCBI nr
Match: NP_001284390.1 (allene oxide synthase 2-like [Cucumis melo] >AAK54282.1 fatty acid 9-hydroperoxide lyase [Cucumis melo])

HSP 1 Score: 905.6 bits (2339), Expect = 1.8e-259
Identity = 448/477 (93.92%), Postives = 461/477 (96.65%), Query Frame = 0

Query: 2   ASSSPELPLKPIPGGYGFPFLGPIKDRYDYFYFQGRDEFFRSRITKYNSTVFHANMPPGP 61
           +SSSPELPLKPIPGGYGFPFLGPIKDRYDYFYFQGRDEFFRSRITKYNSTVF ANMPPGP
Sbjct: 5   SSSSPELPLKPIPGGYGFPFLGPIKDRYDYFYFQGRDEFFRSRITKYNSTVFRANMPPGP 64

Query: 62  FISSDSRVVVLLDALSFPILFDTTKVEKRNILDGTYMPSLSFTGGIRTCAYLDPSETEHT 121
           FISSDSRVVVLLDALSFPILFDT KVEKRNILDGTYMPSLSFTG IRTCAYLDPSETEH+
Sbjct: 65  FISSDSRVVVLLDALSFPILFDTAKVEKRNILDGTYMPSLSFTGNIRTCAYLDPSETEHS 124

Query: 122 VLKRLFLSFLASRHDRFIPLFRSSLSEMFVKLEDKLADKNKIADFNSISDAMSFDYVFRL 181
           VLKRLFLSFLASRHDRFIPLFRSSLSEMFVKLEDKL++K KIADFNSISD+MSFDYVFRL
Sbjct: 125 VLKRLFLSFLASRHDRFIPLFRSSLSEMFVKLEDKLSEKKKIADFNSISDSMSFDYVFRL 184

Query: 182 FSDGTPDSTLAADGPGMFDLWLGLQLAPLASIGLPKIFSVFEDLIIHTIPLPFFPVKSRY 241
            SDGTPDS LAA+GPGMFDLWL  QLAPLASIGLPKIFSVFEDL+IHTIPLPFFPVKS Y
Sbjct: 185 LSDGTPDSKLAAEGPGMFDLWLVFQLAPLASIGLPKIFSVFEDLVIHTIPLPFFPVKSGY 244

Query: 242 RKLYKAFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMKVLFPTILKWVGTGGE 301
           RKLY+AFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMKVLFPT+LKWVGT GE
Sbjct: 245 RKLYEAFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMKVLFPTLLKWVGTAGE 304

Query: 302 DLHRKLAEEVRTAVKEEGGLTFSALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIQS 361
           DLHRKLAEEVRT VKEEGGLTFSALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIQS
Sbjct: 305 DLHRKLAEEVRTTVKEEGGLTFSALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIQS 364

Query: 362 HDSCFKIKKGETIFGYQPFATKDPKIFKDSEKFVGDRFVGEEGEKLLKYVYWSNERETVE 421
           HDS FKIKKGETIFGYQPFATKDPKIFKDSEKFVGDRFVGEEGEKLLKYVYWSNERETVE
Sbjct: 365 HDSSFKIKKGETIFGYQPFATKDPKIFKDSEKFVGDRFVGEEGEKLLKYVYWSNERETVE 424

Query: 422 PTAENKQCPGKNLVVMMGRIIVVEFFLRYDTFTVDVADLALGPAVKFKSLTRATASV 479
           PT ENKQCPGKNLVV++GRI+VVEFFLRYDTFTV+VADL LGPAVKFKSLTRAT  V
Sbjct: 425 PTPENKQCPGKNLVVLIGRIMVVEFFLRYDTFTVEVADLPLGPAVKFKSLTRATDMV 481

BLAST of CSPI07G08060 vs. NCBI nr
Match: XP_038886895.1 (allene oxide synthase 3-like [Benincasa hispida])

HSP 1 Score: 893.6 bits (2308), Expect = 7.2e-256
Identity = 440/477 (92.24%), Postives = 454/477 (95.18%), Query Frame = 0

Query: 2   ASSSPELPLKPIPGGYGFPFLGPIKDRYDYFYFQGRDEFFRSRITKYNSTVFHANMPPGP 61
           +SSSPELPLKPIPGGYGF FLGPIKDRYDYFYFQG+DEFFRSR+TKY STVF ANMPPGP
Sbjct: 5   SSSSPELPLKPIPGGYGFSFLGPIKDRYDYFYFQGKDEFFRSRVTKYGSTVFRANMPPGP 64

Query: 62  FISSDSRVVVLLDALSFPILFDTTKVEKRNILDGTYMPSLSFTGGIRTCAYLDPSETEHT 121
           FISSDSRVVVLLDA+SFPILFDTTKVEKRNILDGTYMPSLSFTGGIRTCAYLDPSETEH+
Sbjct: 65  FISSDSRVVVLLDAVSFPILFDTTKVEKRNILDGTYMPSLSFTGGIRTCAYLDPSETEHS 124

Query: 122 VLKRLFLSFLASRHDRFIPLFRSSLSEMFVKLEDKLADKNKIADFNSISDAMSFDYVFRL 181
           VLK LFLSFLASRHDR IPLFRSSLSEMFVKLEDKLA+K KIADFN ISD+MSFDYVFRL
Sbjct: 125 VLKGLFLSFLASRHDRIIPLFRSSLSEMFVKLEDKLAEKKKIADFNPISDSMSFDYVFRL 184

Query: 182 FSDGTPDSTLAADGPGMFDLWLGLQLAPLASIGLPKIFSVFEDLIIHTIPLPFFPVKSRY 241
           FSDG PD  LAADGPGMFDLWL  QLAPLASIG+P IFS+FEDLIIHTIPLPFFPVKS Y
Sbjct: 185 FSDGNPDPKLAADGPGMFDLWLAFQLAPLASIGVPNIFSIFEDLIIHTIPLPFFPVKSGY 244

Query: 242 RKLYKAFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMKVLFPTILKWVGTGGE 301
           RKLYKAFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMKVLFPTILKWVGT GE
Sbjct: 245 RKLYKAFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMKVLFPTILKWVGTAGE 304

Query: 302 DLHRKLAEEVRTAVKEEGGLTFSALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIQS 361
           DLHRKLA+EVRT VKEEGGLTF ALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVI S
Sbjct: 305 DLHRKLADEVRTTVKEEGGLTFGALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIHS 364

Query: 362 HDSCFKIKKGETIFGYQPFATKDPKIFKDSEKFVGDRFVGEEGEKLLKYVYWSNERETVE 421
           HDS FKIKKGETIFGYQPFATKDPKIFKD+EKFVGDRF GEEGEKLLKYVYWSNERETVE
Sbjct: 365 HDSAFKIKKGETIFGYQPFATKDPKIFKDAEKFVGDRFEGEEGEKLLKYVYWSNERETVE 424

Query: 422 PTAENKQCPGKNLVVMMGRIIVVEFFLRYDTFTVDVADLALGPAVKFKSLTRATASV 479
           PTAENKQCPGKNLVV+MGRII+VEFFLRYDTFTVDVADL LGPAVKFKSLT+AT  V
Sbjct: 425 PTAENKQCPGKNLVVLMGRIILVEFFLRYDTFTVDVADLPLGPAVKFKSLTKATVKV 481

BLAST of CSPI07G08060 vs. TAIR 10
Match: AT5G42650.1 (allene oxide synthase )

HSP 1 Score: 508.8 bits (1309), Expect = 4.6e-144
Identity = 253/475 (53.26%), Postives = 332/475 (69.89%), Query Frame = 0

Query: 3   SSSPELPLKPIPGGYGFPFLGPIKDRYDYFYFQGRDEFFRSRITKYNSTVFHANMPPGPF 62
           + S +LP++ IPG YG P +GPIKDR+DYFY QG +EFF+SRI KYNSTV+  NMPPG F
Sbjct: 47  TGSKDLPIRNIPGNYGLPIVGPIKDRWDYFYDQGAEEFFKSRIRKYNSTVYRVNMPPGAF 106

Query: 63  ISSDSRVVVLLDALSFPILFDTTKVEKRNILDGTYMPSLSFTGGIRTCAYLDPSETEHTV 122
           I+ + +VV LLD  SFP+LFD  KVEK+++  GTYMPS   TGG R  +YLDPSE +H  
Sbjct: 107 IAENPQVVALLDGKSFPVLFDVDKVEKKDLFTGTYMPSTELTGGYRILSYLDPSEPKHEK 166

Query: 123 LKRLFLSFLASRHDRFIPLFRSSLSEMFVKLEDKLADKNKIADFNSISDAMSFDYVFRLF 182
           LK L    L S  +R  P F+++ SE+F  LE +L+ K K ADF   SD  +F+++ R F
Sbjct: 167 LKNLLFFLLKSSRNRIFPEFQATYSELFDSLEKELSLKGK-ADFGGSSDGTAFNFLARAF 226

Query: 183 SDGTP-DSTLAADGPGMFDLWLGLQLAPLASIGLPKIFSVFEDLIIHTIPLPFFPVKSRY 242
               P D+ L AD PG+   W+   L PL SIGLP+   V E+ +IHT  LP   VKS Y
Sbjct: 227 YGTNPADTKLKADAPGLITKWVLFNLHPLLSIGLPR---VIEEPLIHTFSLPPALVKSDY 286

Query: 243 RKLYKAFYSSSGSFLDEAEKQGIDREKACHNLVFLAGFNAYGGMKVLFPTILKWVGTGGE 302
           ++LY+ F  S+G  L EA+K GI RE+A HNL+F   FN +GGMK+LFP ++K +G  G 
Sbjct: 287 QRLYEFFLESAGEILVEADKLGISREEATHNLLFATCFNTWGGMKILFPNMVKRIGRAGH 346

Query: 303 DLHRKLAEEVRTAVKEEGG-LTFSALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIQ 362
            +H +LAEE+R+ +K  GG LT  A+EKM L KSVVYE LR EPPV  QYG+AK+D+VI+
Sbjct: 347 QVHNRLAEEIRSVIKSNGGELTMGAIEKMELTKSVVYECLRFEPPVTAQYGRAKKDLVIE 406

Query: 363 SHDSCFKIKKGETIFGYQPFATKDPKIFKDSEKFVGDRFVGEEGEKLLKYVYWSNERETV 422
           SHD+ FK+K GE ++GYQP AT+DPKIF  +++FV +RFVGEEGEKLL++V WSN  ET 
Sbjct: 407 SHDAAFKVKAGEMLYGYQPLATRDPKIFDRADEFVPERFVGEEGEKLLRHVLWSNGPETE 466

Query: 423 EPTAENKQCPGKNLVVMMGRIIVVEFFLRYDTFTVDVADLALGPAVKFKSLTRAT 476
            PT  NKQC GK+ VV++ R+ V+E F RYD+F ++V    LG +V F SL +A+
Sbjct: 467 TPTVGNKQCAGKDFVVLVARLFVIEIFRRYDSFDIEVGTSPLGSSVNFSSLRKAS 517

BLAST of CSPI07G08060 vs. TAIR 10
Match: AT4G15440.1 (hydroperoxide lyase 1 )

HSP 1 Score: 259.2 bits (661), Expect = 6.4e-69
Identity = 134/373 (35.92%), Postives = 212/373 (56.84%), Query Frame = 0

Query: 87  VEKRNILDGTYMPSLSFTGGIRTCAYLDPSETEHTVLKRLFLSFLASRHDRFIPLFRSSL 146
           V+KR++L G + PSL F GG+R   YLD +E +H  +K   +  L      ++   RS+L
Sbjct: 4   VDKRDVLIGDFRPSLGFYGGVRVGVYLDTTEPKHAKIKGFAMETLKRSSKVWLQELRSNL 63

Query: 147 SEMFVKLEDKLADKNKIADFNSISDAMSFDYVFRLFS--DGTPDSTLAADGPGMFDLWLG 206
           +  +  +E +++ KN  A +        F ++    +  D +    +A +G    + WL 
Sbjct: 64  NIFWGTIESEIS-KNGAASYIFPLQRCIFSFLCASLAGVDASVSPDIAENGWKTINTWLA 123

Query: 207 LQLAPLASIGLPKIFSVFEDLIIHTIPLPFFPVKSRYRKLYKAFYSSSGSFLDEAEKQ-G 266
           LQ+ P A +G+  +    E++++HT P P   +   Y+KLY     ++G  L   +++ G
Sbjct: 124 LQVIPTAKLGV--VPQPLEEILLHTWPYPSLLIAGNYKKLYNFIDENAGDCLRLGQEEFG 183

Query: 267 IDREKACHNLVFLAGFNAYGGMKVLFPTILKWVGTGGEDLHRKLAEEVRTAVKEEGGLTF 326
           + R++A  NL+F+ GFNAYGG  V  P+++  +      L  ++  EVR        L F
Sbjct: 184 LTRDEAIQNLLFVLGFNAYGGFSVFLPSLIGRITGDNSGLQERIRTEVRRVCGSGSDLNF 243

Query: 327 SALEKMSLLKSVVYEALRIEPPVPFQYGKAKEDIVIQSHDSCFKIKKGETIFGYQPFATK 386
             + +M L+KSVVYE LR  PPVP Q+ +A++D  I SHD+ F++KKGE + GYQP   +
Sbjct: 244 KTVNEMELVKSVVYETLRFSPPVPLQFARARKDFQISSHDAVFEVKKGELLCGYQPLVMR 303

Query: 387 DPKIFKDSEKFVGDRFVGEEGEKLLKYVYWSNERETVEPTAENKQCPGKNLVVMMGRIIV 446
           D  +F + E+F  DR+VGE G +LL Y+YWSN  +T  P+A NKQC  K++V +   ++V
Sbjct: 304 DANVFDEPEEFKPDRYVGETGSELLNYLYWSNGPQTGTPSASNKQCAAKDIVTLTASLLV 363

Query: 447 VEFFLRYDTFTVD 457
            + FLRYDT T D
Sbjct: 364 ADLFLRYDTITGD 373

BLAST of CSPI07G08060 vs. TAIR 10
Match: AT5G25180.1 (cytochrome P450, family 71, subfamily B, polypeptide 14 )

HSP 1 Score: 55.5 bits (132), Expect = 1.4e-07
Identity = 40/148 (27.03%), Postives = 70/148 (47.30%), Query Frame = 0

Query: 305 RKLAEEVRTAVKEEGGLTFSALEKMSLLKSVVYEALRIEPPVPFQYGK-AKEDIVIQSHD 364
           +K   EVR  +K +  +T   +E++  LK V+ E LRI P VP    + A + I I  +D
Sbjct: 321 KKAQAEVREVIKNKDDITEEDIERLEYLKMVIKETLRINPLVPLLIPREASKYIKIGGYD 380

Query: 365 SCFKIKKGETIFGYQPFATKDPKIFKDSEKFVGDRFVGEEGEKLLKYVYWSNERETVEPT 424
               I K   I+       ++P ++KD E F+ +RF+  E +      Y   + E +   
Sbjct: 381 ----IPKKTWIYVNIWAVQRNPNVWKDPEVFIPERFMHSEID------YKGVDFELLPFG 440

Query: 425 AENKQCPGKNLVVMMGRIIVVEFFLRYD 452
           +  + CPG  L + +  + ++    R+D
Sbjct: 441 SGRRMCPGMGLGMALVHLTLINLLYRFD 458

BLAST of CSPI07G08060 vs. TAIR 10
Match: AT3G53280.1 (cytochrome p450 71b5 )

HSP 1 Score: 53.9 bits (128), Expect = 4.1e-07
Identity = 56/188 (29.79%), Postives = 82/188 (43.62%), Query Frame = 0

Query: 265 DREKACHNLVFLAGFNAYGGMKVLFPTILKWVGTGGEDLHRKLAEEVRTAV-KEEGGLTF 324
           D  K   + VFLAG NA     +   T L    +    + RKL EE+R A+   +  +T 
Sbjct: 287 DHLKGLMSDVFLAGVNAGSITMIWTMTEL----SRHPRVMRKLQEEIRAALGPNKEKITE 346

Query: 325 SALEKMSLLKSVVYEALRIEPPVPFQYGK-AKEDIVIQSHDSCFKIKKGETIFGYQPFAT 384
             LEK+  LK V+ EA R+ PP P    +    DI IQ     + I K   I        
Sbjct: 347 EDLEKVEYLKMVIEEAFRLHPPAPLLLPRLTMSDINIQG----YSIPKNTMIQINTYTIG 406

Query: 385 KDPKIFKDSEKFVGDRFVGEEGEKLLKYVYWSNERETVEPTAENKQCPGKN---LVVMMG 444
           +DPK +   ++F+ +RFV    E      Y     E +   A  + CPG      +V +G
Sbjct: 407 RDPKNWTKPDEFIPERFVDNPIE------YKGQHFELLPFGAGRRVCPGMATGITIVELG 460

Query: 445 RIIVVEFF 448
            + ++ FF
Sbjct: 467 LLSLLYFF 460

BLAST of CSPI07G08060 vs. TAIR 10
Match: AT3G01900.1 (cytochrome P450, family 94, subfamily B, polypeptide 2 )

HSP 1 Score: 50.8 bits (120), Expect = 3.5e-06
Identity = 29/68 (42.65%), Postives = 42/68 (61.76%), Query Frame = 0

Query: 291 TILKWVGTGGEDLHRKLAEEVRTAVKEE--GGLTFSALEKMSLLKSVVYEALRIEPPVPF 350
           T L W+ TG E+    L  E+R +VKEE  GG  + +L+K+SLLK+ + E +R+ PPVP+
Sbjct: 301 TRLFWLITGHEETEHDLVSEIR-SVKEEITGGFDYESLKKLSLLKACLCEVMRLYPPVPW 360

Query: 351 QYGKAKED 357
               A  D
Sbjct: 361 DSKHALTD 367

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8GZP51.1e-16157.56Allene oxide synthase 3 OS=Solanum lycopersicum OX=4081 GN=AOS3 PE=1 SV=1[more]
K4BV526.3e-14653.85Allene oxide synthase 1, chloroplastic OS=Solanum lycopersicum OX=4081 GN=AOS1 P... [more]
Q7XYS33.8e-14352.95Allene oxide synthase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP74A2 PE=2... [more]
Q962426.5e-14353.26Allene oxide synthase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CYP74A P... [more]
P484171.5e-14251.78Allene oxide synthase, chloroplastic OS=Linum usitatissimum OX=4006 GN=CYP74A PE... [more]
Match NameE-valueIdentityDescription
U5YTM63.0e-27699.79Hydroperoxide lyase OS=Cucumis sativus OX=3659 GN=HPL PE=2 SV=1[more]
Q9M5J23.3e-27599.37Fatty acid hydroperoxide lyase OS=Cucumis sativus OX=3659 PE=2 SV=1[more]
A0A5D3C5U22.3e-26094.13Allene oxide synthase 2-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 G... [more]
A0A1S3BZK92.3e-26094.13allene oxide synthase 2-like isoform X1 OS=Cucumis melo OX=3656 GN=HPL PE=4 SV=1[more]
Q93XR38.9e-26093.92Fatty acid 9-hydroperoxide lyase OS=Cucumis melo OX=3656 GN=HPL PE=2 SV=1[more]
Match NameE-valueIdentityDescription
NP_001274399.16.3e-27699.79allene oxide synthase, chloroplastic-like [Cucumis sativus] >XP_011658536.1 alle... [more]
AAF64041.16.9e-27599.37fatty acid hydroperoxide lyase [Cucumis sativus][more]
XP_008454885.14.8e-26094.13PREDICTED: allene oxide synthase 2-like isoform X1 [Cucumis melo] >KAA0031259.1 ... [more]
NP_001284390.11.8e-25993.92allene oxide synthase 2-like [Cucumis melo] >AAK54282.1 fatty acid 9-hydroperoxi... [more]
XP_038886895.17.2e-25692.24allene oxide synthase 3-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT5G42650.14.6e-14453.26allene oxide synthase [more]
AT4G15440.16.4e-6935.92hydroperoxide lyase 1 [more]
AT5G25180.11.4e-0727.03cytochrome P450, family 71, subfamily B, polypeptide 14 [more]
AT3G53280.14.1e-0729.79cytochrome p450 71b5 [more]
AT3G01900.13.5e-0642.65cytochrome P450, family 94, subfamily B, polypeptide 2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002403Cytochrome P450, E-class, group IVPRINTSPR00465EP450IVcoord: 329..345
score: 37.78
coord: 429..447
score: 20.85
coord: 382..400
score: 31.58
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 8..477
e-value: 1.2E-108
score: 366.0
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 9..456
IPR001128Cytochrome P450PFAMPF00067p450coord: 303..412
e-value: 1.2E-16
score: 60.6
NoneNo IPR availablePANTHERPTHR24286:SF302HYDROPEROXIDE LYASEcoord: 7..455
NoneNo IPR availablePANTHERPTHR24286CYTOCHROME P450 26coord: 7..455

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI07G08060.1CSPI07G08060.1mRNA
CSPI07G08060.2CSPI07G08060.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016125 sterol metabolic process
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0016829 lyase activity
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen