CSPI07G07940 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI07G07940
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
LocationChr7: 5657909 .. 5659927 (-)
RNA-Seq ExpressionCSPI07G07940
SyntenyCSPI07G07940
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTCCACCTTCACTCCTCCTAACTCCTTAACAACAACAGTGGCCATAATAATCTCATCAACGATCATTGCTTTACACTTACAGTTTCATCGTACACGCTTCAACCATGGACCAAGACACCTTGGTCGTGGATTCTTCCTTCATCCAACCCATCCAGCACCGCCCCAAGCTCTATCCCTTCCAACCGGCCACCGACCAAGTCCCAACCATAGATCTCTCCATCTCCCCCTCTCGCACCACCCAACACCTTGTCTCCGAAATAGCCTCTGCCTGCCAGAACTGGGGTTTCTTCCAGGTAATCAACCATGGAGTTTCACTCGAGACGCTCGCGCGATTCGAGAAAGCTGCCAAGTTATTTTTTGGCCAGACGATGGAGGAGAAAAGGAAGGTGAAGAGAGATGCAGGTAATGCTGTGGGATTTTATGACGGTGAGAATACGAAAAACGTTAGAGATTGGAAGGAGGTCTTTGATTTCTTGGTCAAGGATGGGACGTTCTTTCCAGCTTCGCATGATCCAAATGATACGAAATTGAAGGTATTGTTTAATCATTGGCCGGAATATCCCCCTGGATTTAGGTAATTTAATTTGCATTTCTGTTCAAATTGTTCCTTGTCTTTAGTTAAATACATGCCGAGAACAGATCCCATATTCTAGAAATTTAGTTCATGCATGTTTTATTTTCTATATTTGGATTACTTGAAGAGAATAAGAGAATTTGGCACACCAACTTTTTGTATAAAAATTCCTCTCTGAAACTCTGATCTTGTATTTCTTCATCTACTGCATGCCTGCTGGAAGAACTTGTGATTGTTGGTTTTCTTGAATAGGGAAATGTGCGAGGAGTATGCCAGAGAGTTGGAATTGCTAGCACATAAGCTACTGAGGTTGATTCTTTTGAGTCTAGGCTTATCTGGTGCTGATGGATTGAAGGGCTATTTCAATGACGAGCAAATGAGCTTGATGAGGTTGAATCGGTACCCTCCATGTCCGTCTCCGGATCTTGTGCTCGGCGTAGGCCGTCACAAAGATTCTGGTGCCTTGACAATCCTTGCTCAAGATGCTGTTGGAGGGCTGCAAGTGAGGAGAAAATCAGATGGACAGTGGATTCCTGTCAAACCAATCCCAAATGCCTATATTGTTAACATTGGCGATGTTGTTCAAGTATATGTTGCATTTTTCTTATTATATAATATTGATACGTATGGAATCTTCTCTGATCACGGTTATCTGAAAAGGATGAGACTTGAAGAAGTGGAAATGACTTCTGAAAATCGTATAGTTGTTTGGTTTAAGAAGAACAACTGATAAGTCTTACATGCTAGCTGATGCTATCTATCTTATCTTACTGCATTAAAAATAATTTTTCACATGTAAAAAATACTTCCAACCTTTTGGAACCATAAAGGACTGTGTGAATCCAAGTGCTTAAAAAGTGGCTTCATATATAGATAAGCTTATAGAATCGTGTGGTAGATAGATTTTTGTGGTTGAGGAGATGAAGCATTATCTCTAGTTATGGAATTTATTATTTTTCTTTTTTGAGTGAAGATGAAGCATCATATCTTAAGTTCGTGGTTTTTGCATGTGAAGGTTTGGAGCAATGACAAATATGAAAGTGTGGAGCACAGAGTGGTGGTGAATAGTGAGAAAGAAAGGTATTCGTTTCCATTCTTCTTCTTTCCAGCTCACCATATGATGGTGAAGCCATTGGAGGAGGTTGTGGATGAGCAAAACCCACCAAAATACAGAGAATACAACTTCGGAAAGTTTATTGCTTCGAGGAGCCACGGTAACGAGAACCAAGAAGTGGAAAACATCCAAATTCATCATTTCAGGGCCAGGCTGCATCAATAATGTCATCACACATTAATGCCTGTATTGCTATTTATTTATTAAATGCATTTAGGGACCATTTTGTCTTTATAATTCAGTGGCTGTAAGTTTGTAACGTCTAATCTTTCTCTCCTCCAACAAAAATATCTCACAATCAATGTTCACCGCTTATCTTACCAAATCTCAT

mRNA sequence

GTCCACCTTCACTCCTCCTAACTCCTTAACAACAACAGTGGCCATAATAATCTCATCAACGATCATTGCTTTACACTTACAGTTTCATCGTACACGCTTCAACCATGGACCAAGACACCTTGGTCGTGGATTCTTCCTTCATCCAACCCATCCAGCACCGCCCCAAGCTCTATCCCTTCCAACCGGCCACCGACCAAGTCCCAACCATAGATCTCTCCATCTCCCCCTCTCGCACCACCCAACACCTTGTCTCCGAAATAGCCTCTGCCTGCCAGAACTGGGGTTTCTTCCAGGTAATCAACCATGGAGTTTCACTCGAGACGCTCGCGCGATTCGAGAAAGCTGCCAAGTTATTTTTTGGCCAGACGATGGAGGAGAAAAGGAAGGTGAAGAGAGATGCAGGTAATGCTGTGGGATTTTATGACGGTGAGAATACGAAAAACGTTAGAGATTGGAAGGAGGTCTTTGATTTCTTGGTCAAGGATGGGACGTTCTTTCCAGCTTCGCATGATCCAAATGATACGAAATTGAAGGTATTGTTTAATCATTGGCCGGAATATCCCCCTGGATTTAGGGAAATGTGCGAGGAGTATGCCAGAGAGTTGGAATTGCTAGCACATAAGCTACTGAGGTTGATTCTTTTGAGTCTAGGCTTATCTGGTGCTGATGGATTGAAGGGCTATTTCAATGACGAGCAAATGAGCTTGATGAGGTTGAATCGGTACCCTCCATGTCCGTCTCCGGATCTTGTGCTCGGCGTAGGCCGTCACAAAGATTCTGGTGCCTTGACAATCCTTGCTCAAGATGCTGTTGGAGGGCTGCAAGTGAGGAGAAAATCAGATGGACAGTGGATTCCTGTCAAACCAATCCCAAATGCCTATATTGTTAACATTGGCGATGTTGTTCAAGTTTGGAGCAATGACAAATATGAAAGTGTGGAGCACAGAGTGGTGGTGAATAGTGAGAAAGAAAGGTATTCGTTTCCATTCTTCTTCTTTCCAGCTCACCATATGATGGTGAAGCCATTGGAGGAGGTTGTGGATGAGCAAAACCCACCAAAATACAGAGAATACAACTTCGGAAAGTTTATTGCTTCGAGGAGCCACGGTAACGAGAACCAAGAAGTGGAAAACATCCAAATTCATCATTTCAGGGCCAGGCTGCATCAATAATGTCATCACACATTAATGCCTGTATTGCTATTTATTTATTAAATGCATTTAGGGACCATTTTGTCTTTATAATTCAGTGGCTGTAAGTTTGTAACGTCTAATCTTTCTCTCCTCCAACAAAAATATCTCACAATCAATGTTCACCGCTTATCTTACCAAATCTCAT

Coding sequence (CDS)

ATGGACCAAGACACCTTGGTCGTGGATTCTTCCTTCATCCAACCCATCCAGCACCGCCCCAAGCTCTATCCCTTCCAACCGGCCACCGACCAAGTCCCAACCATAGATCTCTCCATCTCCCCCTCTCGCACCACCCAACACCTTGTCTCCGAAATAGCCTCTGCCTGCCAGAACTGGGGTTTCTTCCAGGTAATCAACCATGGAGTTTCACTCGAGACGCTCGCGCGATTCGAGAAAGCTGCCAAGTTATTTTTTGGCCAGACGATGGAGGAGAAAAGGAAGGTGAAGAGAGATGCAGGTAATGCTGTGGGATTTTATGACGGTGAGAATACGAAAAACGTTAGAGATTGGAAGGAGGTCTTTGATTTCTTGGTCAAGGATGGGACGTTCTTTCCAGCTTCGCATGATCCAAATGATACGAAATTGAAGGTATTGTTTAATCATTGGCCGGAATATCCCCCTGGATTTAGGGAAATGTGCGAGGAGTATGCCAGAGAGTTGGAATTGCTAGCACATAAGCTACTGAGGTTGATTCTTTTGAGTCTAGGCTTATCTGGTGCTGATGGATTGAAGGGCTATTTCAATGACGAGCAAATGAGCTTGATGAGGTTGAATCGGTACCCTCCATGTCCGTCTCCGGATCTTGTGCTCGGCGTAGGCCGTCACAAAGATTCTGGTGCCTTGACAATCCTTGCTCAAGATGCTGTTGGAGGGCTGCAAGTGAGGAGAAAATCAGATGGACAGTGGATTCCTGTCAAACCAATCCCAAATGCCTATATTGTTAACATTGGCGATGTTGTTCAAGTTTGGAGCAATGACAAATATGAAAGTGTGGAGCACAGAGTGGTGGTGAATAGTGAGAAAGAAAGGTATTCGTTTCCATTCTTCTTCTTTCCAGCTCACCATATGATGGTGAAGCCATTGGAGGAGGTTGTGGATGAGCAAAACCCACCAAAATACAGAGAATACAACTTCGGAAAGTTTATTGCTTCGAGGAGCCACGGTAACGAGAACCAAGAAGTGGAAAACATCCAAATTCATCATTTCAGGGCCAGGCTGCATCAATAA

Protein sequence

MDQDTLVVDSSFIQPIQHRPKLYPFQPATDQVPTIDLSISPSRTTQHLVSEIASACQNWGFFQVINHGVSLETLARFEKAAKLFFGQTMEEKRKVKRDAGNAVGFYDGENTKNVRDWKEVFDFLVKDGTFFPASHDPNDTKLKVLFNHWPEYPPGFREMCEEYARELELLAHKLLRLILLSLGLSGADGLKGYFNDEQMSLMRLNRYPPCPSPDLVLGVGRHKDSGALTILAQDAVGGLQVRRKSDGQWIPVKPIPNAYIVNIGDVVQVWSNDKYESVEHRVVVNSEKERYSFPFFFFPAHHMMVKPLEEVVDEQNPPKYREYNFGKFIASRSHGNENQEVENIQIHHFRARLHQ*
Homology
BLAST of CSPI07G07940 vs. ExPASy Swiss-Prot
Match: Q9ZSA7 (Protein DMR6-LIKE OXYGENASE 2 OS=Arabidopsis thaliana OX=3702 GN=DLO2 PE=2 SV=1)

HSP 1 Score: 208.4 bits (529), Expect = 1.4e-52
Identity = 118/329 (35.87%), Postives = 177/329 (53.80%), Query Frame = 0

Query: 8   VDSSFIQPIQHRPKLYPFQPATDQVPTIDLSISPSRTTQHLVSEIASACQNWGFFQVINH 67
           V S++++P+  RPK+   Q + D +P IDL          ++++ A AC + GFFQ+ NH
Sbjct: 18  VPSNYVRPVSDRPKMSEVQTSGDSIPLIDLHDLHGPNRADIINQFAHACSSCGFFQIKNH 77

Query: 68  GVSLETLARFEKAAKLFFGQTMEEKRK-----VKRDAGNAVGF-YDGENTKNVRDWKEVF 127
           GV  ET+ +   AA+ FF Q+  E+ K      K+    +  F    E   N RD+  + 
Sbjct: 78  GVPEETIKKMMNAAREFFRQSESERVKHYSADTKKTTRLSTSFNVSKEKVSNWRDFLRLH 137

Query: 128 DFLVKDGTFFPASHDPNDTKLKVLFNHWPEYPPGFREMCEEYARELELLAHKLLRLILLS 187
            + ++D                   N WP  P  FRE+  EYA  +  L   LL  I  S
Sbjct: 138 CYPIED-----------------FINEWPSTPISFREVTAEYATSVRALVLTLLEAISES 197

Query: 188 LGLSGADGLKGYFNDEQMSLMRLNRYPPCPSPDLVLGVGRHKDSGALTILAQDAVGGLQV 247
           LGL+  D +           M +N YP CP P+L  G+  HKD+  +T+L QD V GLQV
Sbjct: 198 LGLA-KDRVSNTIGKHGQH-MAINYYPRCPQPELTYGLPGHKDANLITVLLQDEVSGLQV 257

Query: 248 RRKSDGQWIPVKPIPNAYIVNIGDVVQVWSNDKYESVEHRVVVNSEKERYSFPFFFFPAH 307
            +  DG+WI V P+PN +IVN+GD +QV SN+KY+SV HR VVNS+ ER S P F+ P+ 
Sbjct: 258 FK--DGKWIAVNPVPNTFIVNLGDQMQVISNEKYKSVLHRAVVNSDMERISIPTFYCPSE 317

Query: 308 HMMVKPLEEVVDEQ--NPPKYREYNFGKF 329
             ++ P +E+++E+  +P  YR + + ++
Sbjct: 318 DAVISPAQELINEEEDSPAIYRNFTYAEY 325

BLAST of CSPI07G07940 vs. ExPASy Swiss-Prot
Match: Q7XZQ6 (Flavonol synthase/flavanone 3-hydroxylase OS=Petroselinum crispum OX=4043 GN=FLS PE=1 SV=1)

HSP 1 Score: 207.2 bits (526), Expect = 3.0e-52
Identity = 116/323 (35.91%), Postives = 173/323 (53.56%), Query Frame = 0

Query: 8   VDSSFIQPIQHRPKLYPFQPATDQVPTIDLSISPSRTTQHLVSEIASACQNWGFFQVINH 67
           + S +I+    +P +   Q    QVP IDL  S + T ++LV  IA A + WG FQV+NH
Sbjct: 19  IPSEYIRSESEQPAVTTMQGVVLQVPVIDLG-SSNNTEENLVELIAEASREWGIFQVVNH 78

Query: 68  GVSLETLARFEKAAKLFFGQTMEEKRKVKRDAG-NAVGFYDGENTKNVRDWKEVFDFLVK 127
           G+  + +A+ +K  K FF    +EK  + +  G   V  Y  +  K +   K   D L  
Sbjct: 79  GIPDDAIAKLQKVGKEFFELPQQEKEVIAKPEGYQGVEGYGTKLQKELGGKKGWVDHLFH 138

Query: 128 DGTFFPASHDPNDTKLKVLFNHWPEYPPGFREMCEEYARELELLAHKLLRLILLSLGLSG 187
               +P        K  V +N WP  PP +RE  EEYA  L  +  KL   + L +GL  
Sbjct: 139 --IIWP--------KSAVNYNFWPNNPPLYREANEEYAVALRGVVDKLFEALSLGIGLEK 198

Query: 188 ADGLKGYFNDEQMSLMRLNRYPPCPSPDLVLGVGRHKDSGALTILAQDAVGGLQVRRKSD 247
            +  K    D+ + ++++N YPPCP PDL LGV  H D  A+TIL  + V GLQV +  D
Sbjct: 199 HELKKASGGDDLIYMLKINYYPPCPRPDLALGVVAHTDMSAITILVPNEVQGLQVHK--D 258

Query: 248 GQWIPVKPIPNAYIVNIGDVVQVWSNDKYESVEHRVVVNSEKERYSFPFFFFPAHHMMVK 307
             W  VK IPNA I++IGD +++ SN KY+SV HR  VN +K R S+P F  P   ++  
Sbjct: 259 DHWYDVKYIPNALIIHIGDQIEIMSNGKYKSVYHRTTVNKDKTRMSWPVFLEPPPELLTG 318

Query: 308 PLEEVVDEQNPPKYREYNFGKFI 330
           P+ +++ ++NP K++   +  ++
Sbjct: 319 PISKLITDENPAKFKTKKYKDYV 328

BLAST of CSPI07G07940 vs. ExPASy Swiss-Prot
Match: Q9ZWQ9 (Flavonol synthase/flavanone 3-hydroxylase OS=Citrus unshiu OX=55188 GN=FLS PE=1 SV=1)

HSP 1 Score: 204.5 bits (519), Expect = 2.0e-51
Identity = 115/319 (36.05%), Postives = 167/319 (52.35%), Query Frame = 0

Query: 8   VDSSFIQPIQHRPKLYPFQPATDQVPTIDLSISPSRTTQHLVSEIASACQNWGFFQVINH 67
           + + FI+P + +P    +     ++PTIDL          LV  IA A + WG FQV NH
Sbjct: 19  IPAEFIRPEKEQPASTTYHGPAPEIPTIDLD---DPVQDRLVRSIAEASREWGIFQVTNH 78

Query: 68  GVSLETLARFEKAAKLFFGQTMEEKRKVKRDA-GNAVGFYDGENTKNVRDWKEVFDFLVK 127
           G+  + + + +   K FF    EEK    R A    V  Y  +  K V   K   D L  
Sbjct: 79  GIPSDLICKLQAVGKEFFELPQEEKEVYSRPADAKDVQGYGTKLQKEVEGKKSWVDHLFH 138

Query: 128 DGTFFPASHDPNDTKLKVLFNHWPEYPPGFREMCEEYARELELLAHKLLRLILLSLGLSG 187
               +P S         + +  WP+ PP +R + EEYA+ +  +  KL   + L LG+ G
Sbjct: 139 --RVWPPS--------SINYRFWPKNPPSYRAVNEEYAKYMREVVDKLFTYLSLGLGVEG 198

Query: 188 ADGLKGYFNDEQMSLMRLNRYPPCPSPDLVLGVGRHKDSGALTILAQDAVGGLQVRRKSD 247
               +    D+   ++++N YPPCP PDL LGV  H D  ALT+L  + V GLQV +  D
Sbjct: 199 GVLKEAAGGDDIEYMLKINYYPPCPRPDLALGVVAHTDLSALTVLVPNEVPGLQVFK--D 258

Query: 248 GQWIPVKPIPNAYIVNIGDVVQVWSNDKYESVEHRVVVNSEKERYSFPFFFFPAHHMMVK 307
            +WI  K IPNA +++IGD +++ SN KY++V HR  VN +K R S+P F  P    +V 
Sbjct: 259 DRWIDAKYIPNALVIHIGDQIEILSNGKYKAVLHRTTVNKDKTRMSWPVFLEPPADTVVG 318

Query: 308 PLEEVVDEQNPPKYREYNF 326
           PL ++VD++NPPKY+   F
Sbjct: 319 PLPQLVDDENPPKYKAKKF 322

BLAST of CSPI07G07940 vs. ExPASy Swiss-Prot
Match: Q9FFF6 (Jasmonate-induced oxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=JOX2 PE=1 SV=1)

HSP 1 Score: 204.1 bits (518), Expect = 2.6e-51
Identity = 117/323 (36.22%), Postives = 169/323 (52.32%), Query Frame = 0

Query: 12  FIQPIQHRPKLYPFQPATDQVPTIDLS---ISPSRTTQHLVSEIASACQNWGFFQVINHG 71
           +I+P   RP      P    +P IDL         +   +++ I+ AC+ WGFFQV+NHG
Sbjct: 42  YIKPASLRPTTTEDAPTATNIPIIDLEGLFSEEGLSDDVIMARISEACRGWGFFQVVNHG 101

Query: 72  VSLETLARFEKAAKLFFGQTMEEKRKVKRDAGNAVGFYD--GENTKNVRDWKEVFDFLVK 131
           V  E +    +  + FF   +  K           G+    G       DW + + FL  
Sbjct: 102 VKPELMDAARENWREFFHMPVNAKETYSNSPRTYEGYGSRLGVEKGASLDWSDYY-FL-- 161

Query: 132 DGTFFPASHDPNDTKLKVLFNHWPEYPPGFREMCEEYARELELLAHKLLRLILLSLGLSG 191
                   H   D      FN WP +PP  RE+ +EY  EL  L+ +++R++  +LGL  
Sbjct: 162 ----HLLPHHLKD------FNKWPSFPPTIREVIDEYGEELVKLSGRIMRVLSTNLGLKE 221

Query: 192 ADGLKGYFNDEQMSLMRLNRYPPCPSPDLVLGVGRHKDSGALTI-LAQDAVGGLQVRRKS 251
               + +  +   + +R+N YP CP P+L LG+  H D G +TI L  D V GLQVR+  
Sbjct: 222 DKFQEAFGGENIGACLRVNYYPKCPRPELALGLSPHSDPGGMTILLPDDQVFGLQVRK-- 281

Query: 252 DGQWIPVKPIPNAYIVNIGDVVQVWSNDKYESVEHRVVVNSEKERYSFPFFFFPAHHMMV 311
           D  WI VKP P+A+IVNIGD +Q+ SN  Y+SVEHRV+VNS+KER S  FF+ P   + +
Sbjct: 282 DDTWITVKPHPHAFIVNIGDQIQILSNSTYKSVEHRVIVNSDKERVSLAFFYNPKSDIPI 341

Query: 312 KPLEEVVDEQNPPKYREYNFGKF 329
           +PL+E+V   NPP Y    F ++
Sbjct: 342 QPLQELVSTHNPPLYPPMTFDQY 349

BLAST of CSPI07G07940 vs. ExPASy Swiss-Prot
Match: Q9ZSA8 (Protein DMR6-LIKE OXYGENASE 1 OS=Arabidopsis thaliana OX=3702 GN=DLO1 PE=1 SV=1)

HSP 1 Score: 203.8 bits (517), Expect = 3.3e-51
Identity = 122/328 (37.20%), Postives = 173/328 (52.74%), Query Frame = 0

Query: 8   VDSSFIQPIQHRPKLYPFQPATDQVPTIDLSISPSRTTQHLVSEIASACQNWGFFQVINH 67
           + S++++PI  RP L   + + D +P IDL          +V ++ASAC  +GFFQ+ NH
Sbjct: 20  IPSNYVRPISDRPNLSEVESSGDSIPLIDLRDLHGPNRAVIVQQLASACSTYGFFQIKNH 79

Query: 68  GVSLETLARFEKAAKLFFGQTMEEKRK-----VKRDAGNAVGFYDG-ENTKNVRDWKEVF 127
           GV   T+ + +  A+ FF Q   E+ K       +    +  F  G +   N RD+  + 
Sbjct: 80  GVPDTTVNKMQTVAREFFHQPESERVKHYSADPTKTTRLSTSFNVGADKVLNWRDFLRLH 139

Query: 128 DFLVKDGTFFPASHDPNDTKLKVLFNHWPEYPPGFREMCEEYARELELLAHKLLRLILLS 187
            F ++D                     WP  P  FRE+  EYA  +  L  +LL  I  S
Sbjct: 140 CFPIED-----------------FIEEWPSSPISFREVTAEYATSVRALVLRLLEAISES 199

Query: 188 LGLSGADGLKGYFNDEQMSLMRLNRYPPCPSPDLVLGVGRHKDSGALTILAQDAVGGLQV 247
           LGL  +D +      +    M  N YPPCP P+L  G+  HKD   +T+L QD V GLQV
Sbjct: 200 LGLE-SDHISNILG-KHAQHMAFNYYPPCPEPELTYGLPGHKDPTVITVLLQDQVSGLQV 259

Query: 248 RRKSDGQWIPVKPIPNAYIVNIGDVVQVWSNDKYESVEHRVVVNSEKERYSFPFFFFPAH 307
            +  D +W+ V PIPN +IVNIGD +QV SNDKY+SV HR VVN+E ER S P F+FP+ 
Sbjct: 260 FK--DDKWVAVSPIPNTFIVNIGDQMQVISNDKYKSVLHRAVVNTENERLSIPTFYFPST 319

Query: 308 HMMVKPLEEVVDEQNP-PKYREYNFGKF 329
             ++ P  E+V+EQ+    YR Y F ++
Sbjct: 320 DAVIGPAHELVNEQDSLAIYRTYPFVEY 326

BLAST of CSPI07G07940 vs. ExPASy TrEMBL
Match: A0A0A0K2S8 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G074980 PE=3 SV=1)

HSP 1 Score: 734.9 bits (1896), Expect = 1.5e-208
Identity = 354/355 (99.72%), Postives = 354/355 (99.72%), Query Frame = 0

Query: 1   MDQDTLVVDSSFIQPIQHRPKLYPFQPATDQVPTIDLSISPSRTTQHLVSEIASACQNWG 60
           MDQDTLVVDSSFIQPIQHRPKLYPFQPATDQVPTIDLSISPSRTTQHLVSEIASACQNWG
Sbjct: 1   MDQDTLVVDSSFIQPIQHRPKLYPFQPATDQVPTIDLSISPSRTTQHLVSEIASACQNWG 60

Query: 61  FFQVINHGVSLETLARFEKAAKLFFGQTMEEKRKVKRDAGNAVGFYDGENTKNVRDWKEV 120
           FFQVINHGVSLETLARFEKAAKLFF QTMEEKRKVKRDAGNAVGFYDGENTKNVRDWKEV
Sbjct: 61  FFQVINHGVSLETLARFEKAAKLFFDQTMEEKRKVKRDAGNAVGFYDGENTKNVRDWKEV 120

Query: 121 FDFLVKDGTFFPASHDPNDTKLKVLFNHWPEYPPGFREMCEEYARELELLAHKLLRLILL 180
           FDFLVKDGTFFPASHDPNDTKLKVLFNHWPEYPPGFREMCEEYARELELLAHKLLRLILL
Sbjct: 121 FDFLVKDGTFFPASHDPNDTKLKVLFNHWPEYPPGFREMCEEYARELELLAHKLLRLILL 180

Query: 181 SLGLSGADGLKGYFNDEQMSLMRLNRYPPCPSPDLVLGVGRHKDSGALTILAQDAVGGLQ 240
           SLGLSGADGLKGYFNDEQMSLMRLNRYPPCPSPDLVLGVGRHKDSGALTILAQDAVGGLQ
Sbjct: 181 SLGLSGADGLKGYFNDEQMSLMRLNRYPPCPSPDLVLGVGRHKDSGALTILAQDAVGGLQ 240

Query: 241 VRRKSDGQWIPVKPIPNAYIVNIGDVVQVWSNDKYESVEHRVVVNSEKERYSFPFFFFPA 300
           VRRKSDGQWIPVKPIPNAYIVNIGDVVQVWSNDKYESVEHRVVVNSEKERYSFPFFFFPA
Sbjct: 241 VRRKSDGQWIPVKPIPNAYIVNIGDVVQVWSNDKYESVEHRVVVNSEKERYSFPFFFFPA 300

Query: 301 HHMMVKPLEEVVDEQNPPKYREYNFGKFIASRSHGNENQEVENIQIHHFRARLHQ 356
           HHMMVKPLEEVVDEQNPPKYREYNFGKFIASRSHGNENQEVENIQIHHFRARLHQ
Sbjct: 301 HHMMVKPLEEVVDEQNPPKYREYNFGKFIASRSHGNENQEVENIQIHHFRARLHQ 355

BLAST of CSPI07G07940 vs. ExPASy TrEMBL
Match: A0A5D3C6D5 (Flavonol synthase/flavanone 3-hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G00190 PE=3 SV=1)

HSP 1 Score: 655.2 bits (1689), Expect = 1.5e-184
Identity = 316/348 (90.80%), Postives = 330/348 (94.83%), Query Frame = 0

Query: 3   QDTLVVDSSFIQPIQHRPKLYPFQPATDQVPTIDLSISPSRTTQHLVSEIASACQNWGFF 62
           +DTLVVDS+FIQPI HRPKL PFQ  TDQVPTIDLSIS SRTTQHLVSEIASACQNWGFF
Sbjct: 2   EDTLVVDSTFIQPIHHRPKLPPFQ-LTDQVPTIDLSISLSRTTQHLVSEIASACQNWGFF 61

Query: 63  QVINHGVSLETLARFEKAAKLFFGQTMEEKRKVKRDAGNAVGFYDGENTKNVRDWKEVFD 122
           QVINHGVSLE LAR +KAAKLFF QT+EEKRKVKRD+GNAVGFYDGENTKNVRDWKEVFD
Sbjct: 62  QVINHGVSLEKLARIQKAAKLFFDQTVEEKRKVKRDSGNAVGFYDGENTKNVRDWKEVFD 121

Query: 123 FLVKDGTFFPASHDPNDTKLKVLFNHWPEYPPGFREMCEEYARELELLAHKLLRLILLSL 182
           FLVKDGTFFPASHDPNDTKL+VLFN WPEYPPGFRE CEEYARELELLAHKLLRLILLSL
Sbjct: 122 FLVKDGTFFPASHDPNDTKLRVLFNQWPEYPPGFRETCEEYARELELLAHKLLRLILLSL 181

Query: 183 GLSGADGLKGYFNDEQMSLMRLNRYPPCPSPDLVLGVGRHKDSGALTILAQDAVGGLQVR 242
           GLS AD LKGYFNDEQ SL+RLNRYPPCPSPDLVLGVGRHKDSGALTILAQDAVGGLQVR
Sbjct: 182 GLSDADALKGYFNDEQTSLVRLNRYPPCPSPDLVLGVGRHKDSGALTILAQDAVGGLQVR 241

Query: 243 RKSDGQWIPVKPIPNAYIVNIGDVVQVWSNDKYESVEHRVVVNSEKERYSFPFFFFPAHH 302
           RKSDG+WIPVKPIPNAYI+NIGDVVQVWSNDKYESVEHRVVVN+EKER+SF FFFFPAHH
Sbjct: 242 RKSDGEWIPVKPIPNAYIINIGDVVQVWSNDKYESVEHRVVVNTEKERFSFAFFFFPAHH 301

Query: 303 MMVKPLEEVVDEQNPPKYREYNFGKFIASRSHGNENQEVENIQIHHFR 351
           MMVKPLEE+VDEQNPPKYREYNFGK+ ASRS GN+ QEVENIQI HFR
Sbjct: 302 MMVKPLEELVDEQNPPKYREYNFGKYFASRSRGNQKQEVENIQIDHFR 348

BLAST of CSPI07G07940 vs. ExPASy TrEMBL
Match: A0A1S3C0D6 (flavonol synthase/flavanone 3-hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103495204 PE=3 SV=1)

HSP 1 Score: 655.2 bits (1689), Expect = 1.5e-184
Identity = 316/348 (90.80%), Postives = 330/348 (94.83%), Query Frame = 0

Query: 3   QDTLVVDSSFIQPIQHRPKLYPFQPATDQVPTIDLSISPSRTTQHLVSEIASACQNWGFF 62
           +DTLVVDS+FIQPI HRPKL PFQ  TDQVPTIDLSIS SRTTQHLVSEIASACQNWGFF
Sbjct: 2   EDTLVVDSTFIQPIHHRPKLPPFQ-LTDQVPTIDLSISLSRTTQHLVSEIASACQNWGFF 61

Query: 63  QVINHGVSLETLARFEKAAKLFFGQTMEEKRKVKRDAGNAVGFYDGENTKNVRDWKEVFD 122
           QVINHGVSLE LAR +KAAKLFF QT+EEKRKVKRD+GNAVGFYDGENTKNVRDWKEVFD
Sbjct: 62  QVINHGVSLEKLARIQKAAKLFFDQTVEEKRKVKRDSGNAVGFYDGENTKNVRDWKEVFD 121

Query: 123 FLVKDGTFFPASHDPNDTKLKVLFNHWPEYPPGFREMCEEYARELELLAHKLLRLILLSL 182
           FLVKDGTFFPASHDPNDTKL+VLFN WPEYPPGFRE CEEYARELELLAHKLLRLILLSL
Sbjct: 122 FLVKDGTFFPASHDPNDTKLRVLFNQWPEYPPGFRETCEEYARELELLAHKLLRLILLSL 181

Query: 183 GLSGADGLKGYFNDEQMSLMRLNRYPPCPSPDLVLGVGRHKDSGALTILAQDAVGGLQVR 242
           GLS AD LKGYFNDEQ SL+RLNRYPPCPSPDLVLGVGRHKDSGALTILAQDAVGGLQVR
Sbjct: 182 GLSDADALKGYFNDEQTSLVRLNRYPPCPSPDLVLGVGRHKDSGALTILAQDAVGGLQVR 241

Query: 243 RKSDGQWIPVKPIPNAYIVNIGDVVQVWSNDKYESVEHRVVVNSEKERYSFPFFFFPAHH 302
           RKSDG+WIPVKPIPNAYI+NIGDVVQVWSNDKYESVEHRVVVN+EKER+SF FFFFPAHH
Sbjct: 242 RKSDGEWIPVKPIPNAYIINIGDVVQVWSNDKYESVEHRVVVNTEKERFSFAFFFFPAHH 301

Query: 303 MMVKPLEEVVDEQNPPKYREYNFGKFIASRSHGNENQEVENIQIHHFR 351
           MMVKPLEE+VDEQNPPKYREYNFGK+ ASRS GN+ QEVENIQI HFR
Sbjct: 302 MMVKPLEELVDEQNPPKYREYNFGKYFASRSRGNQKQEVENIQIDHFR 348

BLAST of CSPI07G07940 vs. ExPASy TrEMBL
Match: A0A6J1EZZ4 (flavonol synthase/flavanone 3-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111437780 PE=3 SV=1)

HSP 1 Score: 567.4 bits (1461), Expect = 4.2e-158
Identity = 272/348 (78.16%), Postives = 305/348 (87.64%), Query Frame = 0

Query: 4   DTLVVDSSFIQPIQHRPKLYPFQPATDQVPTIDLSISPSRTTQHLVSEIASACQNWGFFQ 63
           + L  D+SFIQPIQHRPKL P  P  D+VPTIDLSIS SR+ + LVS+IASAC+NWGFFQ
Sbjct: 3   EALAFDASFIQPIQHRPKLEPVDP-IDEVPTIDLSISDSRSVEQLVSDIASACENWGFFQ 62

Query: 64  VINHGVSLETLARFEKAAKLFFGQTMEEKRKVKRDAGNAVGFYDGENTKNVRDWKEVFDF 123
           VINHGV +E +AR E+  KLFF Q +EEKRKVKRDA NA+G++DGENTKNVRDWKEVFDF
Sbjct: 63  VINHGVPMELVARMEEVTKLFFDQGVEEKRKVKRDAANAMGYHDGENTKNVRDWKEVFDF 122

Query: 124 LVKDGTFFPASHDPNDTKLKVLFNHWPEYPPGFREMCEEYARELELLAHKLLRLILLSLG 183
           LVKD T  PASH+P+DT L+VL N WP+YPPGFRE CEEYA+ELELLA+KLL LI LSLG
Sbjct: 123 LVKDRTLVPASHEPDDTNLRVLTNQWPQYPPGFRETCEEYAKELELLAYKLLGLICLSLG 182

Query: 184 LSGADGLKGYFNDEQMSLMRLNRYPPCPSPDLVLGVGRHKDSGALTILAQDAVGGLQVRR 243
           L  AD LKGYF DEQMS++RLNRYPPC SPDLVLGVGRHKD+GALTILAQD VGGLQVRR
Sbjct: 183 LPIADRLKGYFQDEQMSMVRLNRYPPCSSPDLVLGVGRHKDAGALTILAQDGVGGLQVRR 242

Query: 244 KSDGQWIPVKPIPNAYIVNIGDVVQVWSNDKYESVEHRVVVNSEKERYSFPFFFFPAHHM 303
           KSDG+WIPVKPI NAYIVNIGD+VQVWSNDKYESVEHRVVVN+EKER+S PFFFFPAHH 
Sbjct: 243 KSDGEWIPVKPISNAYIVNIGDIVQVWSNDKYESVEHRVVVNTEKERFSIPFFFFPAHHT 302

Query: 304 MVKPLEEVVDEQNPPKYREYNFGKFIASRSHGN-ENQEVENIQIHHFR 351
           MVKPLEEVV+EQNPPKYREYNFGKF ASR+  + + Q+VEN+QI HFR
Sbjct: 303 MVKPLEEVVNEQNPPKYREYNFGKFFASRNRSDFQKQKVENVQIDHFR 349

BLAST of CSPI07G07940 vs. ExPASy TrEMBL
Match: A0A6J1JE50 (flavonol synthase/flavanone 3-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111486062 PE=3 SV=1)

HSP 1 Score: 560.1 bits (1442), Expect = 6.8e-156
Identity = 270/348 (77.59%), Postives = 303/348 (87.07%), Query Frame = 0

Query: 4   DTLVVDSSFIQPIQHRPKLYPFQPATDQVPTIDLSISPSRTTQHLVSEIASACQNWGFFQ 63
           + L  D+SFIQPI+HRPKL    P  D+VPTIDLSIS SR+ + LVS+IASAC+NWGFFQ
Sbjct: 3   EALAFDASFIQPIEHRPKLEHVDP-IDEVPTIDLSISDSRSVEQLVSDIASACENWGFFQ 62

Query: 64  VINHGVSLETLARFEKAAKLFFGQTMEEKRKVKRDAGNAVGFYDGENTKNVRDWKEVFDF 123
           VINHGV +E LAR EK AKLFF Q +EEKRKVKRDA NA+G++DGENTKNVRDWKEVFDF
Sbjct: 63  VINHGVPMELLARMEKVAKLFFDQGIEEKRKVKRDAANAMGYHDGENTKNVRDWKEVFDF 122

Query: 124 LVKDGTFFPASHDPNDTKLKVLFNHWPEYPPGFREMCEEYARELELLAHKLLRLILLSLG 183
           LV+D T  PASH+P+ T L+VL N WP+YPP FRE CEEYA+ELELLA+KLL LI LSLG
Sbjct: 123 LVRDRTLVPASHEPDGTSLRVLTNQWPQYPPEFRETCEEYAKELELLAYKLLGLICLSLG 182

Query: 184 LSGADGLKGYFNDEQMSLMRLNRYPPCPSPDLVLGVGRHKDSGALTILAQDAVGGLQVRR 243
           L  AD LKGYF DEQMS++RLNRYPPC SPDLVLGVGRHKD+GALTILAQD VGGLQVRR
Sbjct: 183 LPIADRLKGYFQDEQMSMVRLNRYPPCSSPDLVLGVGRHKDAGALTILAQDGVGGLQVRR 242

Query: 244 KSDGQWIPVKPIPNAYIVNIGDVVQVWSNDKYESVEHRVVVNSEKERYSFPFFFFPAHHM 303
           KSDG+WIPVKPI NAYIVNIGD+VQVWSNDKYESVEHRVVVN+EKER+S PFFFFPAHH 
Sbjct: 243 KSDGEWIPVKPISNAYIVNIGDIVQVWSNDKYESVEHRVVVNTEKERFSIPFFFFPAHHT 302

Query: 304 MVKPLEEVVDEQNPPKYREYNFGKFIASRSHGN-ENQEVENIQIHHFR 351
           MVKPLEEVV+EQNPPKYREYNFGKF ASR+  + + Q+VEN+QI HFR
Sbjct: 303 MVKPLEEVVNEQNPPKYREYNFGKFFASRNRSDFQKQKVENVQIDHFR 349

BLAST of CSPI07G07940 vs. NCBI nr
Match: XP_004136998.1 (flavonol synthase/flavanone 3-hydroxylase [Cucumis sativus] >KGN43965.1 hypothetical protein Csa_017221 [Cucumis sativus])

HSP 1 Score: 734.9 bits (1896), Expect = 3.2e-208
Identity = 354/355 (99.72%), Postives = 354/355 (99.72%), Query Frame = 0

Query: 1   MDQDTLVVDSSFIQPIQHRPKLYPFQPATDQVPTIDLSISPSRTTQHLVSEIASACQNWG 60
           MDQDTLVVDSSFIQPIQHRPKLYPFQPATDQVPTIDLSISPSRTTQHLVSEIASACQNWG
Sbjct: 1   MDQDTLVVDSSFIQPIQHRPKLYPFQPATDQVPTIDLSISPSRTTQHLVSEIASACQNWG 60

Query: 61  FFQVINHGVSLETLARFEKAAKLFFGQTMEEKRKVKRDAGNAVGFYDGENTKNVRDWKEV 120
           FFQVINHGVSLETLARFEKAAKLFF QTMEEKRKVKRDAGNAVGFYDGENTKNVRDWKEV
Sbjct: 61  FFQVINHGVSLETLARFEKAAKLFFDQTMEEKRKVKRDAGNAVGFYDGENTKNVRDWKEV 120

Query: 121 FDFLVKDGTFFPASHDPNDTKLKVLFNHWPEYPPGFREMCEEYARELELLAHKLLRLILL 180
           FDFLVKDGTFFPASHDPNDTKLKVLFNHWPEYPPGFREMCEEYARELELLAHKLLRLILL
Sbjct: 121 FDFLVKDGTFFPASHDPNDTKLKVLFNHWPEYPPGFREMCEEYARELELLAHKLLRLILL 180

Query: 181 SLGLSGADGLKGYFNDEQMSLMRLNRYPPCPSPDLVLGVGRHKDSGALTILAQDAVGGLQ 240
           SLGLSGADGLKGYFNDEQMSLMRLNRYPPCPSPDLVLGVGRHKDSGALTILAQDAVGGLQ
Sbjct: 181 SLGLSGADGLKGYFNDEQMSLMRLNRYPPCPSPDLVLGVGRHKDSGALTILAQDAVGGLQ 240

Query: 241 VRRKSDGQWIPVKPIPNAYIVNIGDVVQVWSNDKYESVEHRVVVNSEKERYSFPFFFFPA 300
           VRRKSDGQWIPVKPIPNAYIVNIGDVVQVWSNDKYESVEHRVVVNSEKERYSFPFFFFPA
Sbjct: 241 VRRKSDGQWIPVKPIPNAYIVNIGDVVQVWSNDKYESVEHRVVVNSEKERYSFPFFFFPA 300

Query: 301 HHMMVKPLEEVVDEQNPPKYREYNFGKFIASRSHGNENQEVENIQIHHFRARLHQ 356
           HHMMVKPLEEVVDEQNPPKYREYNFGKFIASRSHGNENQEVENIQIHHFRARLHQ
Sbjct: 301 HHMMVKPLEEVVDEQNPPKYREYNFGKFIASRSHGNENQEVENIQIHHFRARLHQ 355

BLAST of CSPI07G07940 vs. NCBI nr
Match: XP_008454898.1 (PREDICTED: flavonol synthase/flavanone 3-hydroxylase-like [Cucumis melo] >KAA0031266.1 flavonol synthase/flavanone 3-hydroxylase-like [Cucumis melo var. makuwa] >TYK06718.1 flavonol synthase/flavanone 3-hydroxylase-like [Cucumis melo var. makuwa])

HSP 1 Score: 655.2 bits (1689), Expect = 3.2e-184
Identity = 316/348 (90.80%), Postives = 330/348 (94.83%), Query Frame = 0

Query: 3   QDTLVVDSSFIQPIQHRPKLYPFQPATDQVPTIDLSISPSRTTQHLVSEIASACQNWGFF 62
           +DTLVVDS+FIQPI HRPKL PFQ  TDQVPTIDLSIS SRTTQHLVSEIASACQNWGFF
Sbjct: 2   EDTLVVDSTFIQPIHHRPKLPPFQ-LTDQVPTIDLSISLSRTTQHLVSEIASACQNWGFF 61

Query: 63  QVINHGVSLETLARFEKAAKLFFGQTMEEKRKVKRDAGNAVGFYDGENTKNVRDWKEVFD 122
           QVINHGVSLE LAR +KAAKLFF QT+EEKRKVKRD+GNAVGFYDGENTKNVRDWKEVFD
Sbjct: 62  QVINHGVSLEKLARIQKAAKLFFDQTVEEKRKVKRDSGNAVGFYDGENTKNVRDWKEVFD 121

Query: 123 FLVKDGTFFPASHDPNDTKLKVLFNHWPEYPPGFREMCEEYARELELLAHKLLRLILLSL 182
           FLVKDGTFFPASHDPNDTKL+VLFN WPEYPPGFRE CEEYARELELLAHKLLRLILLSL
Sbjct: 122 FLVKDGTFFPASHDPNDTKLRVLFNQWPEYPPGFRETCEEYARELELLAHKLLRLILLSL 181

Query: 183 GLSGADGLKGYFNDEQMSLMRLNRYPPCPSPDLVLGVGRHKDSGALTILAQDAVGGLQVR 242
           GLS AD LKGYFNDEQ SL+RLNRYPPCPSPDLVLGVGRHKDSGALTILAQDAVGGLQVR
Sbjct: 182 GLSDADALKGYFNDEQTSLVRLNRYPPCPSPDLVLGVGRHKDSGALTILAQDAVGGLQVR 241

Query: 243 RKSDGQWIPVKPIPNAYIVNIGDVVQVWSNDKYESVEHRVVVNSEKERYSFPFFFFPAHH 302
           RKSDG+WIPVKPIPNAYI+NIGDVVQVWSNDKYESVEHRVVVN+EKER+SF FFFFPAHH
Sbjct: 242 RKSDGEWIPVKPIPNAYIINIGDVVQVWSNDKYESVEHRVVVNTEKERFSFAFFFFPAHH 301

Query: 303 MMVKPLEEVVDEQNPPKYREYNFGKFIASRSHGNENQEVENIQIHHFR 351
           MMVKPLEE+VDEQNPPKYREYNFGK+ ASRS GN+ QEVENIQI HFR
Sbjct: 302 MMVKPLEELVDEQNPPKYREYNFGKYFASRSRGNQKQEVENIQIDHFR 348

BLAST of CSPI07G07940 vs. NCBI nr
Match: XP_038888870.1 (flavonol synthase/flavanone 3-hydroxylase [Benincasa hispida])

HSP 1 Score: 605.9 bits (1561), Expect = 2.2e-169
Identity = 290/351 (82.62%), Postives = 317/351 (90.31%), Query Frame = 0

Query: 4   DTLVVDSSFIQPIQHRPKLYPFQPATDQVPTIDLSISPSRTTQHLVSEIASACQNWGFFQ 63
           +TL +DS+FIQPI+HRPKL P  P  ++VPTIDLSIS SRTTQ LVSEIASACQNWGFFQ
Sbjct: 3   ETLAMDSTFIQPIEHRPKLEPLNPINEEVPTIDLSISLSRTTQQLVSEIASACQNWGFFQ 62

Query: 64  VINHGVSLETLARFEKAAKLFFGQTMEEKRKVKRDAGNAVGFYDGENTKNVRDWKEVFDF 123
           VINHGV LE LAR +K AKLFF QT+EEKRKVKRDA NA+GF+DGENTKNVRDWKEVFDF
Sbjct: 63  VINHGVPLELLARIKKTAKLFFDQTVEEKRKVKRDAANAMGFHDGENTKNVRDWKEVFDF 122

Query: 124 LVKDGTFFPASHDPNDTKLKVLFNHWPEYPPGFREMCEEYARELELLAHKLLRLILLSLG 183
           LVKD TF PASHDPND KL+VL N WP+YPPGFRE CE+YARELELLAHKLL LI LSLG
Sbjct: 123 LVKDETFIPASHDPNDKKLRVLRNQWPQYPPGFRETCEDYARELELLAHKLLGLISLSLG 182

Query: 184 LSGADGLKGYFNDEQMSLMRLNRYPPCPSPDLVLGVGRHKDSGALTILAQDAVGGLQVRR 243
           LSGADGLKGYFNDEQ SL+RLN YPPCPSPDLVLGVGRHKD+GALTILAQD VGGLQV+R
Sbjct: 183 LSGADGLKGYFNDEQTSLVRLNHYPPCPSPDLVLGVGRHKDAGALTILAQDGVGGLQVKR 242

Query: 244 KSDGQWIPVKPIPNAYIVNIGDVVQVWSNDKYESVEHRVVVNSEKERYSFPFFFFPAHHM 303
           KSD +WIPVKP+PNA+IVNIGDVVQVWSNDKYESVEHRVVVN+EKER+SFPFFFFPAHHM
Sbjct: 243 KSDEEWIPVKPVPNAFIVNIGDVVQVWSNDKYESVEHRVVVNTEKERFSFPFFFFPAHHM 302

Query: 304 MVKPLEEVVDEQNPPKYREYNFGKFIASRSHGN-ENQEVENIQIHHFRARL 354
           MVKPLEE+V+EQNPPKYREYNFGKF ASR+  + + Q+VENIQI HFR  L
Sbjct: 303 MVKPLEELVNEQNPPKYREYNFGKFFASRNRSDFQKQKVENIQIDHFRLPL 353

BLAST of CSPI07G07940 vs. NCBI nr
Match: KAG7022434.1 (Protein DMR6-LIKE OXYGENASE 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 568.5 bits (1464), Expect = 3.9e-158
Identity = 272/348 (78.16%), Postives = 306/348 (87.93%), Query Frame = 0

Query: 4   DTLVVDSSFIQPIQHRPKLYPFQPATDQVPTIDLSISPSRTTQHLVSEIASACQNWGFFQ 63
           + L  D+SFIQPIQHRPKL P  P  D+VPTIDLSIS SR+ + LVS+IASAC+NWGFFQ
Sbjct: 3   EALAFDASFIQPIQHRPKLEPVDP-IDEVPTIDLSISDSRSVEQLVSDIASACENWGFFQ 62

Query: 64  VINHGVSLETLARFEKAAKLFFGQTMEEKRKVKRDAGNAVGFYDGENTKNVRDWKEVFDF 123
           VINHGV +E +AR E+  KLFF Q ++EKRKVKRDA NA+G++DGENTKNVRDWKEVFDF
Sbjct: 63  VINHGVPMELVARMEEVTKLFFDQGVDEKRKVKRDAANAMGYHDGENTKNVRDWKEVFDF 122

Query: 124 LVKDGTFFPASHDPNDTKLKVLFNHWPEYPPGFREMCEEYARELELLAHKLLRLILLSLG 183
           LVKD T  PASH+P+DT L+VL N WP+YPPGFRE CEEYA+ELELLA+KLL LI LSLG
Sbjct: 123 LVKDRTLVPASHEPDDTNLRVLTNQWPQYPPGFRETCEEYAKELELLAYKLLGLICLSLG 182

Query: 184 LSGADGLKGYFNDEQMSLMRLNRYPPCPSPDLVLGVGRHKDSGALTILAQDAVGGLQVRR 243
           LS AD LKGYF DEQMS++RLNRYPPC SPDLVLGVGRHKD+GALTILAQD VGGLQVRR
Sbjct: 183 LSVADRLKGYFQDEQMSMVRLNRYPPCSSPDLVLGVGRHKDAGALTILAQDGVGGLQVRR 242

Query: 244 KSDGQWIPVKPIPNAYIVNIGDVVQVWSNDKYESVEHRVVVNSEKERYSFPFFFFPAHHM 303
           KSDG+WIPVKPI NAYIVNIGD+VQVWSNDKYESVEHRVVVN+EKER+S PFFFFPAHH 
Sbjct: 243 KSDGEWIPVKPISNAYIVNIGDIVQVWSNDKYESVEHRVVVNTEKERFSIPFFFFPAHHT 302

Query: 304 MVKPLEEVVDEQNPPKYREYNFGKFIASRSHGN-ENQEVENIQIHHFR 351
           MVKPLEEVV+EQNPPKYREYNFGKF ASR+  + + Q+VEN+QI HFR
Sbjct: 303 MVKPLEEVVNEQNPPKYREYNFGKFFASRNRSDFQKQKVENVQIDHFR 349

BLAST of CSPI07G07940 vs. NCBI nr
Match: XP_022931615.1 (flavonol synthase/flavanone 3-hydroxylase-like [Cucurbita moschata])

HSP 1 Score: 567.4 bits (1461), Expect = 8.8e-158
Identity = 272/348 (78.16%), Postives = 305/348 (87.64%), Query Frame = 0

Query: 4   DTLVVDSSFIQPIQHRPKLYPFQPATDQVPTIDLSISPSRTTQHLVSEIASACQNWGFFQ 63
           + L  D+SFIQPIQHRPKL P  P  D+VPTIDLSIS SR+ + LVS+IASAC+NWGFFQ
Sbjct: 3   EALAFDASFIQPIQHRPKLEPVDP-IDEVPTIDLSISDSRSVEQLVSDIASACENWGFFQ 62

Query: 64  VINHGVSLETLARFEKAAKLFFGQTMEEKRKVKRDAGNAVGFYDGENTKNVRDWKEVFDF 123
           VINHGV +E +AR E+  KLFF Q +EEKRKVKRDA NA+G++DGENTKNVRDWKEVFDF
Sbjct: 63  VINHGVPMELVARMEEVTKLFFDQGVEEKRKVKRDAANAMGYHDGENTKNVRDWKEVFDF 122

Query: 124 LVKDGTFFPASHDPNDTKLKVLFNHWPEYPPGFREMCEEYARELELLAHKLLRLILLSLG 183
           LVKD T  PASH+P+DT L+VL N WP+YPPGFRE CEEYA+ELELLA+KLL LI LSLG
Sbjct: 123 LVKDRTLVPASHEPDDTNLRVLTNQWPQYPPGFRETCEEYAKELELLAYKLLGLICLSLG 182

Query: 184 LSGADGLKGYFNDEQMSLMRLNRYPPCPSPDLVLGVGRHKDSGALTILAQDAVGGLQVRR 243
           L  AD LKGYF DEQMS++RLNRYPPC SPDLVLGVGRHKD+GALTILAQD VGGLQVRR
Sbjct: 183 LPIADRLKGYFQDEQMSMVRLNRYPPCSSPDLVLGVGRHKDAGALTILAQDGVGGLQVRR 242

Query: 244 KSDGQWIPVKPIPNAYIVNIGDVVQVWSNDKYESVEHRVVVNSEKERYSFPFFFFPAHHM 303
           KSDG+WIPVKPI NAYIVNIGD+VQVWSNDKYESVEHRVVVN+EKER+S PFFFFPAHH 
Sbjct: 243 KSDGEWIPVKPISNAYIVNIGDIVQVWSNDKYESVEHRVVVNTEKERFSIPFFFFPAHHT 302

Query: 304 MVKPLEEVVDEQNPPKYREYNFGKFIASRSHGN-ENQEVENIQIHHFR 351
           MVKPLEEVV+EQNPPKYREYNFGKF ASR+  + + Q+VEN+QI HFR
Sbjct: 303 MVKPLEEVVNEQNPPKYREYNFGKFFASRNRSDFQKQKVENVQIDHFR 349

BLAST of CSPI07G07940 vs. TAIR 10
Match: AT3G19000.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 416.0 bits (1068), Expect = 3.0e-116
Identity = 203/353 (57.51%), Postives = 259/353 (73.37%), Query Frame = 0

Query: 8   VDSSFIQPIQHRPKLYPFQPA----TDQVPTIDLSISPSRTTQH----LVSEIASACQNW 67
           +D +FIQ  +HRP  +         +D++PTIDL  S    T H    +  EIA AC+ W
Sbjct: 4   LDEAFIQAPEHRPNTHLTNSGDFIFSDEIPTIDL--SSLEDTHHDKTAIAKEIAEACKRW 63

Query: 68  GFFQVINHGVSLETLARFEKAAKLFFGQTMEEKRKVKRDAGNAVGFYDGENTKNVRDWKE 127
           GFFQVINHG+      R EK A  FF  T EEKRKVKRD  N +G++D E+TKNVRDWKE
Sbjct: 64  GFFQVINHGLPSALRHRVEKTAAEFFNLTTEEKRKVKRDEVNPMGYHDEEHTKNVRDWKE 123

Query: 128 VFDFLVKDGTFFPASHDPNDTKLKVLFNHWPEYPPGFREMCEEYARELELLAHKLLRLIL 187
           +FDF ++D T  PAS +P DT+L+ L N WP+ P  FRE+C+EYARE+E LA +LL L+ 
Sbjct: 124 IFDFFLQDSTIVPASPEPEDTELRKLTNQWPQNPSHFREVCQEYAREVEKLAFRLLELVS 183

Query: 188 LSLGLSGADGLKGYFNDEQMSLMRLNRYPPCPSPDLVLGVGRHKDSGALTILAQDAVGGL 247
           +SLGL G D L G+FN EQ S +R N YPPCP+P+L LGVGRHKD GALT+LAQD+VGGL
Sbjct: 184 ISLGLPG-DRLTGFFN-EQTSFLRFNHYPPCPNPELALGVGRHKDGGALTVLAQDSVGGL 243

Query: 248 QVRRKSDGQWIPVKPIPNAYIVNIGDVVQVWSNDKYESVEHRVVVNSEKERYSFPFFFFP 307
           QV R+SDGQWIPVKPI +A I+N+G+ +QVW+ND+Y S EHRVVVN+ KER+S PFFFFP
Sbjct: 244 QVSRRSDGQWIPVKPISDALIINMGNCIQVWTNDEYWSAEHRVVVNTSKERFSIPFFFFP 303

Query: 308 AHHMMVKPLEEVVDEQNPPKYREYNFGKFIASRSHGN-ENQEVENIQIHHFRA 352
           +H   ++PLEE++ E+NPP Y++YN+GKF  SR+  + +  EVENIQI HF+A
Sbjct: 304 SHEANIEPLEELISEENPPCYKKYNWGKFFVSRNRSDFKKLEVENIQIDHFKA 352

BLAST of CSPI07G07940 vs. TAIR 10
Match: AT3G19010.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 386.3 bits (991), Expect = 2.6e-107
Identity = 187/349 (53.58%), Postives = 243/349 (69.63%), Query Frame = 0

Query: 8   VDSSFIQPIQHR--PKLYPFQPATDQVPTIDLS-ISPSRTTQHLVSEIASACQNWGFFQV 67
           +D ++IQ  +HR      P QP  +++P IDLS +      Q+++SEI  AC+ WGFFQV
Sbjct: 4   LDPTYIQAPEHRSDSNFIPNQP--EEIPVIDLSRLDDPEDVQNVISEIGDACEKWGFFQV 63

Query: 68  INHGVSLETLARFEKAAKLFFGQTMEEKRKVKRDAGNAVGFYDGENTKNVRDWKEVFDFL 127
           +NHGV  +   R EK  K+FF   MEEK KVKRD  N VG++DGE+TKNV+DWKEVFD  
Sbjct: 64  LNHGVPSDARQRVEKTVKMFFDLPMEEKIKVKRDDVNPVGYHDGEHTKNVKDWKEVFDIY 123

Query: 128 VKDGTFFPASHDPNDTKLKVLFNHWPEYPPGFREMCEEYARELELLAHKLLRLILLSLGL 187
            KD    P++ DP D  L++++N WP+ P  FRE CE YAR  E LA KLL LI LSLGL
Sbjct: 124 FKDPMVIPSTTDPEDEGLRLVYNKWPQSPSDFREACEVYARHAEKLAFKLLELISLSLGL 183

Query: 188 SGADGLKGYFNDEQMSLMRLNRYPPCPSPDLVLGVGRHKDSGALTILAQDAVGGLQVRRK 247
              +    YF  EQMS  R+NRYPPCP PDL LGVG HKD+  +++LAQD VGGLQV R+
Sbjct: 184 P-KERFHDYFK-EQMSFFRINRYPPCPRPDLALGVGHHKDADVISLLAQDDVGGLQVSRR 243

Query: 248 SDGQWIPVKPIPNAYIVNIGDVVQVWSNDKYESVEHRVVVNSEKERYSFPFFFFPAHHMM 307
           SDG W P++P+PNA ++NIG+ +++W+NDKY S EHRVVVN+ +ERYS PFF  P+H + 
Sbjct: 244 SDGVWFPIRPVPNALVINIGNCMEIWTNDKYWSAEHRVVVNTTRERYSIPFFLLPSHDVE 303

Query: 308 VKPLEEVVDEQNPPKYREYNFGKFIASRSHGN-ENQEVENIQIHHFRAR 353
           VKPLEE+V  +NPPKY+ Y +GKF  SR+  +    E+ENIQI  F+ +
Sbjct: 304 VKPLEELVSPENPPKYKGYKWGKFYVSRNRSDFRKLEIENIQIDDFKVK 348

BLAST of CSPI07G07940 vs. TAIR 10
Match: AT3G19010.3 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 350.5 bits (898), Expect = 1.6e-96
Identity = 170/349 (48.71%), Postives = 225/349 (64.47%), Query Frame = 0

Query: 8   VDSSFIQPIQHR--PKLYPFQPATDQVPTIDLS-ISPSRTTQHLVSEIASACQNWGFFQV 67
           +D ++IQ  +HR      P QP  +++P IDLS +      Q+++SEI  AC+ WGFFQV
Sbjct: 4   LDPTYIQAPEHRSDSNFIPNQP--EEIPVIDLSRLDDPEDVQNVISEIGDACEKWGFFQV 63

Query: 68  INHGVSLETLARFEKAAKLFFGQTMEEKRKVKRDAGNAVGFYDGENTKNVRDWKEVFDFL 127
           +NHGV  +   R EK  K+FF   MEEK KVKRD  N VG++DGE+TKNV+DWKEVFD  
Sbjct: 64  LNHGVPSDARQRVEKTVKMFFDLPMEEKIKVKRDDVNPVGYHDGEHTKNVKDWKEVFDIY 123

Query: 128 VKDGTFFPASHDPNDTKLKVLFNHWPEYPPGFREMCEEYARELELLAHKLLRLILLSLGL 187
            KD    P++ DP D  L++++N WP+ P  FRE CE YAR  E LA             
Sbjct: 124 FKDPMVIPSTTDPEDEGLRLVYNKWPQSPSDFREACEVYARHAEKLA------------- 183

Query: 188 SGADGLKGYFNDEQMSLMRLNRYPPCPSPDLVLGVGRHKDSGALTILAQDAVGGLQVRRK 247
                              +NRYPPCP PDL LGVG HKD+  +++LAQD VGGLQV R+
Sbjct: 184 -------------------INRYPPCPRPDLALGVGHHKDADVISLLAQDDVGGLQVSRR 243

Query: 248 SDGQWIPVKPIPNAYIVNIGDVVQVWSNDKYESVEHRVVVNSEKERYSFPFFFFPAHHMM 307
           SDG W P++P+PNA ++NIG+ +++W+NDKY S EHRVVVN+ +ERYS PFF  P+H + 
Sbjct: 244 SDGVWFPIRPVPNALVINIGNCMEIWTNDKYWSAEHRVVVNTTRERYSIPFFLLPSHDVE 303

Query: 308 VKPLEEVVDEQNPPKYREYNFGKFIASRSHGN-ENQEVENIQIHHFRAR 353
           VKPLEE+V  +NPPKY+ Y +GKF  SR+  +    E+ENIQI  F+ +
Sbjct: 304 VKPLEELVSPENPPKYKGYKWGKFYVSRNRSDFRKLEIENIQIDDFKVK 318

BLAST of CSPI07G07940 vs. TAIR 10
Match: AT3G19000.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 309.3 bits (791), Expect = 4.0e-84
Identity = 154/270 (57.04%), Postives = 193/270 (71.48%), Query Frame = 0

Query: 8   VDSSFIQPIQHRPKLYPFQPA----TDQVPTIDLSISPSRTTQH----LVSEIASACQNW 67
           +D +FIQ  +HRP  +         +D++PTIDL  S    T H    +  EIA AC+ W
Sbjct: 4   LDEAFIQAPEHRPNTHLTNSGDFIFSDEIPTIDL--SSLEDTHHDKTAIAKEIAEACKRW 63

Query: 68  GFFQVINHGVSLETLARFEKAAKLFFGQTMEEKRKVKRDAGNAVGFYDGENTKNVRDWKE 127
           GFFQVINHG+      R EK A  FF  T EEKRKVKRD  N +G++D E+TKNVRDWKE
Sbjct: 64  GFFQVINHGLPSALRHRVEKTAAEFFNLTTEEKRKVKRDEVNPMGYHDEEHTKNVRDWKE 123

Query: 128 VFDFLVKDGTFFPASHDPNDTKLKVLFNHWPEYPPGFREMCEEYARELELLAHKLLRLIL 187
           +FDF ++D T  PAS +P DT+L+ L N WP+ P  FRE+C+EYARE+E LA +LL L+ 
Sbjct: 124 IFDFFLQDSTIVPASPEPEDTELRKLTNQWPQNPSHFREVCQEYAREVEKLAFRLLELVS 183

Query: 188 LSLGLSGADGLKGYFNDEQMSLMRLNRYPPCPSPDLVLGVGRHKDSGALTILAQDAVGGL 247
           +SLGL G D L G+FN EQ S +R N YPPCP+P+L LGVGRHKD GALT+LAQD+VGGL
Sbjct: 184 ISLGLPG-DRLTGFFN-EQTSFLRFNHYPPCPNPELALGVGRHKDGGALTVLAQDSVGGL 243

Query: 248 QVRRKSDGQWIPVKPIPNAYIVNIGDVVQV 270
           QV R+SDGQWIPVKPI +A I+N+G+ +QV
Sbjct: 244 QVSRRSDGQWIPVKPISDALIINMGNCIQV 269

BLAST of CSPI07G07940 vs. TAIR 10
Match: AT3G19010.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 288.1 bits (736), Expect = 9.6e-78
Identity = 141/265 (53.21%), Postives = 182/265 (68.68%), Query Frame = 0

Query: 8   VDSSFIQPIQHR--PKLYPFQPATDQVPTIDLS-ISPSRTTQHLVSEIASACQNWGFFQV 67
           +D ++IQ  +HR      P QP  +++P IDLS +      Q+++SEI  AC+ WGFFQV
Sbjct: 4   LDPTYIQAPEHRSDSNFIPNQP--EEIPVIDLSRLDDPEDVQNVISEIGDACEKWGFFQV 63

Query: 68  INHGVSLETLARFEKAAKLFFGQTMEEKRKVKRDAGNAVGFYDGENTKNVRDWKEVFDFL 127
           +NHGV  +   R EK  K+FF   MEEK KVKRD  N VG++DGE+TKNV+DWKEVFD  
Sbjct: 64  LNHGVPSDARQRVEKTVKMFFDLPMEEKIKVKRDDVNPVGYHDGEHTKNVKDWKEVFDIY 123

Query: 128 VKDGTFFPASHDPNDTKLKVLFNHWPEYPPGFREMCEEYARELELLAHKLLRLILLSLGL 187
            KD    P++ DP D  L++++N WP+ P  FRE CE YAR  E LA KLL LI LSLGL
Sbjct: 124 FKDPMVIPSTTDPEDEGLRLVYNKWPQSPSDFREACEVYARHAEKLAFKLLELISLSLGL 183

Query: 188 SGADGLKGYFNDEQMSLMRLNRYPPCPSPDLVLGVGRHKDSGALTILAQDAVGGLQVRRK 247
              +    YF  EQMS  R+NRYPPCP PDL LGVG HKD+  +++LAQD VGGLQV R+
Sbjct: 184 P-KERFHDYFK-EQMSFFRINRYPPCPRPDLALGVGHHKDADVISLLAQDDVGGLQVSRR 243

Query: 248 SDGQWIPVKPIPNAYIVNIGDVVQV 270
           SDG W P++P+PNA ++NIG+ ++V
Sbjct: 244 SDGVWFPIRPVPNALVINIGNCMEV 264

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9ZSA71.4e-5235.87Protein DMR6-LIKE OXYGENASE 2 OS=Arabidopsis thaliana OX=3702 GN=DLO2 PE=2 SV=1[more]
Q7XZQ63.0e-5235.91Flavonol synthase/flavanone 3-hydroxylase OS=Petroselinum crispum OX=4043 GN=FLS... [more]
Q9ZWQ92.0e-5136.05Flavonol synthase/flavanone 3-hydroxylase OS=Citrus unshiu OX=55188 GN=FLS PE=1 ... [more]
Q9FFF62.6e-5136.22Jasmonate-induced oxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=JOX2 PE=1 SV=1[more]
Q9ZSA83.3e-5137.20Protein DMR6-LIKE OXYGENASE 1 OS=Arabidopsis thaliana OX=3702 GN=DLO1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0K2S81.5e-20899.72Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G... [more]
A0A5D3C6D51.5e-18490.80Flavonol synthase/flavanone 3-hydroxylase-like OS=Cucumis melo var. makuwa OX=11... [more]
A0A1S3C0D61.5e-18490.80flavonol synthase/flavanone 3-hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103... [more]
A0A6J1EZZ44.2e-15878.16flavonol synthase/flavanone 3-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1JE506.8e-15677.59flavonol synthase/flavanone 3-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LO... [more]
Match NameE-valueIdentityDescription
XP_004136998.13.2e-20899.72flavonol synthase/flavanone 3-hydroxylase [Cucumis sativus] >KGN43965.1 hypothet... [more]
XP_008454898.13.2e-18490.80PREDICTED: flavonol synthase/flavanone 3-hydroxylase-like [Cucumis melo] >KAA003... [more]
XP_038888870.12.2e-16982.62flavonol synthase/flavanone 3-hydroxylase [Benincasa hispida][more]
KAG7022434.13.9e-15878.16Protein DMR6-LIKE OXYGENASE 1 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022931615.18.8e-15878.16flavonol synthase/flavanone 3-hydroxylase-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT3G19000.13.0e-11657.512-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT3G19010.12.6e-10753.582-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT3G19010.31.6e-9648.712-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT3G19000.24.0e-8457.042-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT3G19010.29.6e-7853.212-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00682IPNSYNTHASEcoord: 52..69
score: 50.51
coord: 287..305
score: 35.41
coord: 222..243
score: 40.91
NoneNo IPR availablePANTHERPTHR10209:SF727DIRIGENT PROTEINcoord: 7..342
NoneNo IPR availablePANTHERPTHR10209OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEINcoord: 7..342
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 10..332
IPR027443Isopenicillin N synthase-like superfamilyGENE3D2.60.120.330coord: 1..340
e-value: 1.4E-105
score: 355.3
IPR044861Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domainPFAMPF031712OG-FeII_Oxycoord: 201..299
e-value: 2.1E-29
score: 102.0
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 32..151
e-value: 4.3E-25
score: 88.8
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 197..299
score: 14.241934

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI07G07940.1CSPI07G07940.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016491 oxidoreductase activity