CSPI07G07650 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI07G07650
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	abscisic acid 8'-hydroxylase 4
Location	Chr7: 5457785 .. 5460668 (+)
RNA-Seq Expression	CSPI07G07650
Synteny	CSPI07G07650

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTATTTAAACCCTCAATCCTTCAACTCATTCCCCTCACTCTCATTTCACAACACTCAAACAAAACTCCCAAGTTTTTCCCACACCTCCAAGAAATAAAAACTCCATTTTACACTCACTCCCTTTCATTGTTATTTTTTTCGTCCTCAAAAAACACAAATTCTAAGAAGAGAAACAGAGAGATTGCTGCATTTTTCTTAATGGAAGCTCTCTCTGTTCCTCTTTACATAACAATACTCCTTCTTCTTCTTTTCATTTTCTTTTCCATTTCATTTCTTAAAAGAACGAGAAATGAAAACCCCAAATCCCAAAATTCTCTTAAACTCCCTCCTGGTTCCATGGGATTACCTTGGATTGGTGAAACTTTGCAACTTTATTCTCAAGATCCCAATATCTTCTTCTCACAGAAACAGAGAAGGTTCCTTCAAAAATATTCAAATTTTTTTCAAACAAAATCTATCCCATAAAGAAACAAAAATGGAAATTTCTAATTGGGTTTCTGTTTTTGGTGACAGATATGGTGAAATTTTTAAGACCAATATTTTGGGGTGTCCTTGTGTTATGTTGGCAAGCGCAGCCGCAGCACGGTTTGTTTTGGTCACAAACGCCCATTTATTCCGACCGACTTACCCCAAAAGTAAAGAGACGATGATCGGACCGGCGGCGCTTTTCTTTCACCAAGGAAACTACCATTCTAACCTCAGGAAGCTTGTTCTTAATTCTCTGTCTCTTGAACGTCTCAGAACTCTTGTTCCTTGTATCGAAGCTGCCGCCATTTCCGCCACCGATTCTTGGGCCGCCGCCGGCCACGTCATCAATACCTTCCTTGAAATGAAAAAGGTCCGTTCTGTAGAAACAGAGAATTTCTTGGAATGGCTTTTTGTTATTAAGTATTCTCCACTAAAGTTATAAATTTTGAATTCTCACTCTCTCTGCACATTACATTGTTGAGAACTAAAAGTTATATCCACGCAGTATATCCTTCGCTTCATTCATCTTTTAGCAGTCCGCAAAAAAAGAAAGATAAATACAACTGAGAATGCAACGAAAACACACCACGTTTAAGTAATATAAAAATGGAATGATTTTTTAATATATATAGAAGTAATATATTTTATGTGTGTATGTGTTTGGTATGAACAGTATTCATTTGAGGTGGGAATTATAGCAGTGTTTGGAAAGCTAGAAGATGAGTACAAAGAGAAGCTAAAACAAAATTATTGCATATTAGACAAAGGCTACAACTGTTTTCCCACCAGATTACCAGGAACAGCATATTCAAAAGCATTATCAGTAAGATTTAGCTCATCCCCAATAATTGATCTTCTCCCAAAAACTTTCCCATTTTGTTTTTCAATCTTATTAATTTTGTTTTTTTAAAAAAAGGCAAGGAAGAAGCTTAGGGAGATTCTGGGAGAGATAATAATGGAAAGAAGAGAAAAAAGAGTAACAGAGAGAGATTTAGTTGGTCATCTTTTGAACTTCAGAGACGAAAATGGTGAAAGCTTAAGTGAAGATCAAATCGCCGATAATATCATCGGAGTTCTCTTCGCCGCCCAAGACACCACCGCTACTGTCCTCACTTGGATCCTTAAATATCTTCACGATAACTTCAAACTTTTCGAGGCTGTCAAGGTAACCATTTTTCTCCATTTTCATTCCAATCATTTCAAAATGAGAATTTATTTTCATAATAAAGGATATAATTTTGATAGCATAACGACTACAGATTATTATTTTGAAAGTTTACGACTAAATCATTATCGATTAGCATTTGGACAAAACCACGTGTGCTCATTTTAACGATGTGTTTTGGTTTATTGGTAAAAAATTTCAGGCAGAGCAGATGGAAATTCATAGAAGAAATGGAGAAGGAAAGATGCCTCTGTCTTGGAGTCAGATTAAGGACATGCCCTTTACTCATAGGGTATGTCTCATTTCTCTCATCCATTCTACCAATACTTCACCAATTAAATATTCATGTCTTTTAACTTTACTGTTATTAATGATGATGATATGCATATGATTATTTTTCAGGTTGTATTAGAGAGCTTGAGGATGGCTAGCATCATATCCTTCACTTTTAGGGAGGCAGTAGTTGATGTGGAATATAAAGGTATATTCCATGGTTCTTTTAAAAATTAATGAAAATATATATCAACATATATAGATATATATATAAAGCGGGTGAATAATTAAAGAAATAATTAATCATATTTATAGGGTACCTTATCCCAAAGGGCTGGAAGGTGATGCCTTTATTCAGGAATATTCATCACAACCATGAGTACTTCCCTGACCCTCATATATTTGATCCCTCTAGATTTGAGGTAAGGCTTTTGAATATTCATATTTGAGGTAAGGTTGTTAAGGAATAAGCTAGCGAAGTTTTGAAATCATTGTTAATTTGGGTATCATGTAGGTGGCTCCAAGGGCCAATACATTCATGCCATTTGGGAATGGAGTACATTCTTGCCCTGGAAATGAACTTGCCAAGCTGGAGATCCTCATATTGCTCCATCACCTCATTACCAAATTCAGGTAAATCCAAACCCCAGAGTATGTAGTCATAAATGTTTTCCTATTGTTATTGTTGTTGATAAAAAATAAAAAAAAAAAGGAAAAGGAATTAAGAAAGGAATGTGTTACGTTTGCAGGTGGGAAGTAGAGGGATCACAAAGTGGGGTTGAATATGGGCCATTCCCAATGCCTGTCCAAGGCCTACCCGCCAGATTTTTCAAACAAACAACAACCAACCAATACCCTTTCCTTTCATAATTCTATATAAAAATCCAATCTCTCTTTTTATTTTTCCAAATCTTCCCAACAATTATTATTATTATTATGTTTCTAAAATTAATGCCCTAAGCCTTCTTCACCTAAACC

mRNA sequence

CTATTTAAACCCTCAATCCTTCAACTCATTCCCCTCACTCTCATTTCACAACACTCAAACAAAACTCCCAAGTTTTTCCCACACCTCCAAGAAATAAAAACTCCATTTTACACTCACTCCCTTTCATTGTTATTTTTTTCGTCCTCAAAAAACACAAATTCTAAGAAGAGAAACAGAGAGATTGCTGCATTTTTCTTAATGGAAGCTCTCTCTGTTCCTCTTTACATAACAATACTCCTTCTTCTTCTTTTCATTTTCTTTTCCATTTCATTTCTTAAAAGAACGAGAAATGAAAACCCCAAATCCCAAAATTCTCTTAAACTCCCTCCTGGTTCCATGGGATTACCTTGGATTGGTGAAACTTTGCAACTTTATTCTCAAGATCCCAATATCTTCTTCTCACAGAAACAGAGAAGATATGGTGAAATTTTTAAGACCAATATTTTGGGGTGTCCTTGTGTTATGTTGGCAAGCGCAGCCGCAGCACGGTTTGTTTTGGTCACAAACGCCCATTTATTCCGACCGACTTACCCCAAAAGTAAAGAGACGATGATCGGACCGGCGGCGCTTTTCTTTCACCAAGGAAACTACCATTCTAACCTCAGGAAGCTTGTTCTTAATTCTCTGTCTCTTGAACGTCTCAGAACTCTTGTTCCTTGTATCGAAGCTGCCGCCATTTCCGCCACCGATTCTTGGGCCGCCGCCGGCCACGTCATCAATACCTTCCTTGAAATGAAAAAGTATTCATTTGAGGTGGGAATTATAGCAGTGTTTGGAAAGCTAGAAGATGAGTACAAAGAGAAGCTAAAACAAAATTATTGCATATTAGACAAAGGCTACAACTGTTTTCCCACCAGATTACCAGGAACAGCATATTCAAAAGCATTATCAGCAAGGAAGAAGCTTAGGGAGATTCTGGGAGAGATAATAATGGAAAGAAGAGAAAAAAGAGTAACAGAGAGAGATTTAGTTGGTCATCTTTTGAACTTCAGAGACGAAAATGGTGAAAGCTTAAGTGAAGATCAAATCGCCGATAATATCATCGGAGTTCTCTTCGCCGCCCAAGACACCACCGCTACTGTCCTCACTTGGATCCTTAAATATCTTCACGATAACTTCAAACTTTTCGAGGCTGTCAAGGCAGAGCAGATGGAAATTCATAGAAGAAATGGAGAAGGAAAGATGCCTCTGTCTTGGAGTCAGATTAAGGACATGCCCTTTACTCATAGGGTTGTATTAGAGAGCTTGAGGATGGCTAGCATCATATCCTTCACTTTTAGGGAGGCAGTAGTTGATGTGGAATATAAAGGGTACCTTATCCCAAAGGGCTGGAAGGTGATGCCTTTATTCAGGAATATTCATCACAACCATGAGTACTTCCCTGACCCTCATATATTTGATCCCTCTAGATTTGAGGTGGCTCCAAGGGCCAATACATTCATGCCATTTGGGAATGGAGTACATTCTTGCCCTGGAAATGAACTTGCCAAGCTGGAGATCCTCATATTGCTCCATCACCTCATTACCAAATTCAGGTGGGAAGTAGAGGGATCACAAAGTGGGGTTGAATATGGGCCATTCCCAATGCCTGTCCAAGGCCTACCCGCCAGATTTTTCAAACAAACAACAACCAACCAATACCCTTTCCTTTCATAATTCTATATAAAAATCCAATCTCTCTTTTTATTTTTCCAAATCTTCCCAACAATTATTATTATTATTATGTTTCTAAAATTAATGCCCTAAGCCTTCTTCACCTAAACC

Coding sequence (CDS)

ATGGAAGCTCTCTCTGTTCCTCTTTACATAACAATACTCCTTCTTCTTCTTTTCATTTTCTTTTCCATTTCATTTCTTAAAAGAACGAGAAATGAAAACCCCAAATCCCAAAATTCTCTTAAACTCCCTCCTGGTTCCATGGGATTACCTTGGATTGGTGAAACTTTGCAACTTTATTCTCAAGATCCCAATATCTTCTTCTCACAGAAACAGAGAAGATATGGTGAAATTTTTAAGACCAATATTTTGGGGTGTCCTTGTGTTATGTTGGCAAGCGCAGCCGCAGCACGGTTTGTTTTGGTCACAAACGCCCATTTATTCCGACCGACTTACCCCAAAAGTAAAGAGACGATGATCGGACCGGCGGCGCTTTTCTTTCACCAAGGAAACTACCATTCTAACCTCAGGAAGCTTGTTCTTAATTCTCTGTCTCTTGAACGTCTCAGAACTCTTGTTCCTTGTATCGAAGCTGCCGCCATTTCCGCCACCGATTCTTGGGCCGCCGCCGGCCACGTCATCAATACCTTCCTTGAAATGAAAAAGTATTCATTTGAGGTGGGAATTATAGCAGTGTTTGGAAAGCTAGAAGATGAGTACAAAGAGAAGCTAAAACAAAATTATTGCATATTAGACAAAGGCTACAACTGTTTTCCCACCAGATTACCAGGAACAGCATATTCAAAAGCATTATCAGCAAGGAAGAAGCTTAGGGAGATTCTGGGAGAGATAATAATGGAAAGAAGAGAAAAAAGAGTAACAGAGAGAGATTTAGTTGGTCATCTTTTGAACTTCAGAGACGAAAATGGTGAAAGCTTAAGTGAAGATCAAATCGCCGATAATATCATCGGAGTTCTCTTCGCCGCCCAAGACACCACCGCTACTGTCCTCACTTGGATCCTTAAATATCTTCACGATAACTTCAAACTTTTCGAGGCTGTCAAGGCAGAGCAGATGGAAATTCATAGAAGAAATGGAGAAGGAAAGATGCCTCTGTCTTGGAGTCAGATTAAGGACATGCCCTTTACTCATAGGGTTGTATTAGAGAGCTTGAGGATGGCTAGCATCATATCCTTCACTTTTAGGGAGGCAGTAGTTGATGTGGAATATAAAGGGTACCTTATCCCAAAGGGCTGGAAGGTGATGCCTTTATTCAGGAATATTCATCACAACCATGAGTACTTCCCTGACCCTCATATATTTGATCCCTCTAGATTTGAGGTGGCTCCAAGGGCCAATACATTCATGCCATTTGGGAATGGAGTACATTCTTGCCCTGGAAATGAACTTGCCAAGCTGGAGATCCTCATATTGCTCCATCACCTCATTACCAAATTCAGGTGGGAAGTAGAGGGATCACAAAGTGGGGTTGAATATGGGCCATTCCCAATGCCTGTCCAAGGCCTACCCGCCAGATTTTTCAAACAAACAACAACCAACCAATACCCTTTCCTTTCATAA

Protein sequence

MEALSVPLYITILLLLLFIFFSISFLKRTRNENPKSQNSLKLPPGSMGLPWIGETLQLYSQDPNIFFSQKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETMIGPAALFFHQGNYHSNLRKLVLNSLSLERLRTLVPCIEAAAISATDSWAAAGHVINTFLEMKKYSFEVGIIAVFGKLEDEYKEKLKQNYCILDKGYNCFPTRLPGTAYSKALSARKKLREILGEIIMERREKRVTERDLVGHLLNFRDENGESLSEDQIADNIIGVLFAAQDTTATVLTWILKYLHDNFKLFEAVKAEQMEIHRRNGEGKMPLSWSQIKDMPFTHRVVLESLRMASIISFTFREAVVDVEYKGYLIPKGWKVMPLFRNIHHNHEYFPDPHIFDPSRFEVAPRANTFMPFGNGVHSCPGNELAKLEILILLHHLITKFRWEVEGSQSGVEYGPFPMPVQGLPARFFKQTTTNQYPFLS*

Homology

[Close]

BLAST of CSPI07G07650 vs. ExPASy Swiss-Prot
Match: Q9LJK2 (Abscisic acid 8'-hydroxylase 4 OS=Arabidopsis thaliana OX=3702 GN=CYP707A4 PE=2 SV=2)

HSP 1 Score: 632.9 bits (1631), Expect = 3.0e-180
Identity = 308/470 (65.53%), Postives = 376/470 (80.00%), Query Frame = 0


Query: 4   LSVPLYITILLLLLFIFFSISFLKRTRNENPKSQNSL-KLPPGSMGLPWIGETLQLYSQD 63
           L VP+ I  LLL+  I             + K +NS  KLPPGSMG P++GETLQLYSQ+
Sbjct: 7   LVVPILILCLLLVRVIV------------SKKKKNSRGKLPPGSMGWPYLGETLQLYSQN 66

Query: 64  PNIFFSQKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETMIGPA 123
           PN+FF+ KQ+RYGEIFKT ILG PCVMLAS  AARFVLVT+AH+F+PTYP+SKE +IGP+
Sbjct: 67  PNVFFTSKQKRYGEIFKTRILGYPCVMLASPEAARFVLVTHAHMFKPTYPRSKEKLIGPS 126

Query: 124 ALFFHQGNYHSNLRKLVLNSLSLERLRTLVPCIEAAAISATDSWAAAGHVINTFLEMKKY 183
           ALFFHQG+YHS++RKLV +S   E +R L+P IE  A+S+  SWA    +++T+ EMKK+
Sbjct: 127 ALFFHQGDYHSHIRKLVQSSFYPETIRKLIPDIEHIALSSLQSWANM-PIVSTYQEMKKF 186

Query: 184 SFEVGIIAVFGKLEDEYKEKLKQNYCILDKGYNCFPTRLPGTAYSKALSARKKLREILGE 243
           +F+VGI+A+FG LE  YKE LK NY I+DKGYN FP  LPGT+Y KAL ARK+L+ I+ E
Sbjct: 187 AFDVGILAIFGHLESSYKEILKHNYNIVDKGYNSFPMSLPGTSYHKALMARKQLKTIVSE 246

Query: 244 IIMERREKRVTERDLVGHLLNFRDENGESLSEDQIADNIIGVLFAAQDTTATVLTWILKY 303
           II ERREKR  + D +GHLLNF++E G  L+++QIADNIIGVLFAAQDTTA+ LTWILKY
Sbjct: 247 IICERREKRALQTDFLGHLLNFKNEKGRVLTQEQIADNIIGVLFAAQDTTASCLTWILKY 306

Query: 304 LHDNFKLFEAVKAEQMEIHRRNGEGKMPLSWSQIKDMPFTHRVVLESLRMASIISFTFRE 363
           LHD+ KL EAVKAEQ  I+  N   K PL+W Q ++MP TH+V++ESLRMASIISFTFRE
Sbjct: 307 LHDDQKLLEAVKAEQKAIYEENSREKKPLTWRQTRNMPLTHKVIVESLRMASIISFTFRE 366

Query: 364 AVVDVEYKGYLIPKGWKVMPLFRNIHHNHEYFPDPHIFDPSRFEVAPRANTFMPFGNGVH 423
           AVVDVEYKGYLIPKGWKVMPLFRNIHHN +YF +P +FDPSRFEV P+ NTFMPFG+GVH
Sbjct: 367 AVVDVEYKGYLIPKGWKVMPLFRNIHHNPKYFSNPEVFDPSRFEVNPKPNTFMPFGSGVH 426

Query: 424 SCPGNELAKLEILILLHHLITKFRWEVEGSQSGVEYGPFPMPVQGLPARF 473
           +CPGNELAKL+ILI LHHL++ FRWEV+G + G++Y PFP+P  GLPA F
Sbjct: 427 ACPGNELAKLQILIFLHHLVSNFRWEVKGGEKGIQYSPFPIPQNGLPATF 463

[Close]

BLAST of CSPI07G07650 vs. ExPASy Swiss-Prot
Match: A9QNE7 (Abscisic acid 8'-hydroxylase CYP707A1 OS=Solanum lycopersicum OX=4081 GN=CYP707A1 PE=2 SV=1)

HSP 1 Score: 629.8 bits (1623), Expect = 2.6e-179
Identity = 313/472 (66.31%), Postives = 373/472 (79.03%), Query Frame = 0


Query: 8   LYITILLLLLFIFFSISFLKRTRNENPKSQNSLKLPPGSMGLPWIGETLQLYSQDPNIFF 67
           LYI++ +L    + S  F     N +   +N+ KLPPGSMG P+IGETLQLYSQDPN FF
Sbjct: 9   LYISMFVL---GYLSYYFCFGKNNNSSSKKNAYKLPPGSMGWPYIGETLQLYSQDPNAFF 68

Query: 68  SQKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETMIGPAALFFH 127
             +QRR+GEIFKT ILGCPCVMLAS  AARFVLV  A+LF+PTYPKSKE +IG +A+FFH
Sbjct: 69  INRQRRFGEIFKTKILGCPCVMLASPEAARFVLVNQANLFKPTYPKSKENLIGQSAIFFH 128

Query: 128 QGNYHSNLRKLVLNSLSLERLRTLVPCIEAAAISATDSWAAAGHVINTFLEMKKYSFEVG 187
           QG+YH++LRKLV   L+ E +R  +P IE  +ISA +SW   GHV+NT+ EMKK+SFEVG
Sbjct: 129 QGDYHNHLRKLVQAPLNPESIRNQIPYIEELSISALNSW-VGGHVVNTYHEMKKFSFEVG 188

Query: 188 IIAVFGKLEDEYKEKLKQNYCILDKGYNCFPTRLPGTAYSKALSARKKLREILGEIIMER 247
           I+A+FG L+   KE+LK+NY I+DKGYN FP  LPGT Y KAL ARKKL +IL EII E 
Sbjct: 189 ILAIFGHLDGHVKEELKKNYSIVDKGYNSFPINLPGTLYRKALQARKKLGKILSEIIREM 248

Query: 248 REKRVTERDLVGHLLNFRDENG-ESLSEDQIADNIIGVLFAAQDTTATVLTWILKYLHDN 307
           +EK+  E+ L+   LN ++E G   L+EDQIADNIIGVLFAAQDTTA+VLTWI+KYLHDN
Sbjct: 249 KEKKTLEKGLLSCFLNAKEEKGFLVLNEDQIADNIIGVLFAAQDTTASVLTWIIKYLHDN 308

Query: 308 FKLFEAVKAEQMEIHRRNGEGKMPLSWSQIKDMPFTHRVVLESLRMASIISFTFREAVVD 367
            KL E VKAEQ  I + N +    L+W+Q + MP T RVVLE+LRMASIISF FREAV D
Sbjct: 309 PKLLECVKAEQKVIWQSNEQENHGLTWTQTRKMPITSRVVLETLRMASIISFAFREAVAD 368

Query: 368 VEYKGYLIPKGWKVMPLFRNIHHNHEYFPDPHIFDPSRFEVAPRANTFMPFGNGVHSCPG 427
           VEYKGYLIPKGWKVMPLFRNIHHN E+FPDP  FDPSRFE AP+ NTFMPFG+GVH+CPG
Sbjct: 369 VEYKGYLIPKGWKVMPLFRNIHHNPEFFPDPQKFDPSRFENAPKPNTFMPFGSGVHACPG 428

Query: 428 NELAKLEILILLHHLITKFRWEVEGSQSGVEYGPFPMPVQGLPARFFKQTTT 479
           NELAKLEILI+ HHL+TKFRWEV GS SG++YGPFP+P+ GL ARF+K T+T
Sbjct: 429 NELAKLEILIMTHHLVTKFRWEVVGSGSGIQYGPFPVPLGGLAARFWKTTST 476

[Close]

BLAST of CSPI07G07650 vs. ExPASy Swiss-Prot
Match: Q09J78 (Abscisic acid 8'-hydroxylase 2 OS=Oryza sativa subsp. indica OX=39946 GN=CYP707A6 PE=2 SV=1)

HSP 1 Score: 594.7 bits (1532), Expect = 9.2e-169
Identity = 283/446 (63.45%), Postives = 354/446 (79.37%), Query Frame = 0


Query: 35  KSQNSLKLPPGSMGLPWIGETLQLYSQDPNIFFSQKQRRYGEIFKTNILGCPCVMLASAA 94
           + +  L+LPPGSMG P++GETLQLYSQDPN+FF+ KQ+RYGEIFKTN+LGCPCVMLAS  
Sbjct: 44  QKKKKLRLPPGSMGWPYVGETLQLYSQDPNVFFASKQKRYGEIFKTNLLGCPCVMLASPE 103

Query: 95  AARFVLVTNAHLFRPTYPKSKETMIGPAALFFHQGNYHSNLRKLVLNSLSLERLRTLVPC 154
           AARFVLV+ A LF+PTYP SKE MIGP+ALFFHQG YH  LR+LV  +L+ + LR LVP 
Sbjct: 104 AARFVLVSQARLFKPTYPPSKERMIGPSALFFHQGEYHLRLRRLVQAALAPDSLRALVPD 163

Query: 155 IEAAAISATDSWAAAGHVINTFLEMKKYSFEVGIIAVF-GKLEDEYKEKLKQNYCILDKG 214
           ++AA  +   +W + GHV +TF  MKK SF+VG++ +F G+L   ++E+L+ NY ++++G
Sbjct: 164 VDAAVAATLAAW-SGGHVASTFHAMKKLSFDVGVVTIFGGRLGRRHREELRTNYSVVERG 223

Query: 215 YNCFPTRLPGTAYSKALSARKKLREILGEIIMERREKRVTERDLVGHLLNFRDENGES-- 274
           YNCFP R PGT Y KA+ ARK+LR IL EI+ ERR +     DL+G L+  RD+      
Sbjct: 224 YNCFPNRFPGTLYHKAIQARKRLRAILSEIVAERRARGGGGDDLLGGLMRSRDDGTAGAV 283

Query: 275 --LSEDQIADNIIGVLFAAQDTTATVLTWILKYLHDNFKLFEAVKAEQMEIHRRNGEGKM 334
             L++DQIADN++GVLFAAQDTTA+VLTWILKYLHD+ KL EAVKAEQM I+  N  GK 
Sbjct: 284 ALLTDDQIADNVVGVLFAAQDTTASVLTWILKYLHDSPKLLEAVKAEQMAIYVANEGGKR 343

Query: 335 PLSWSQIKDMPFTHRVVLESLRMASIISFTFREAVVDVEYKGYLIPKGWKVMPLFRNIHH 394
           PL+W+Q + M  TH+V+LESLRMASIISFTFREAV DVEYKG+LIPKGWKVMPLFRNIHH
Sbjct: 344 PLTWTQTRSMTLTHQVILESLRMASIISFTFREAVADVEYKGFLIPKGWKVMPLFRNIHH 403

Query: 395 NHEYFPDPHIFDPSRFEVAPRANTFMPFGNGVHSCPGNELAKLEILILLHHLITKFRWEV 454
           N +YF DP  FDPSRF+VAPR +TF+PFG+GVH+CPGNELAKLE+L+L+H L+T +RWE+
Sbjct: 404 NPDYFQDPQKFDPSRFKVAPRPSTFLPFGSGVHACPGNELAKLEMLVLVHRLVTAYRWEI 463

Query: 455 EGSQSGVEYGPFPMPVQGLPARFFKQ 476
            G+   VEY PFP+P  GL A+ +KQ
Sbjct: 464 VGASDEVEYSPFPVPRGGLNAKLWKQ 488

[Close]

BLAST of CSPI07G07650 vs. ExPASy Swiss-Prot
Match: Q0J185 (Abscisic acid 8'-hydroxylase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP707A7 PE=2 SV=1)

HSP 1 Score: 594.0 bits (1530), Expect = 1.6e-168
Identity = 287/478 (60.04%), Postives = 369/478 (77.20%), Query Frame = 0


Query: 12  ILLLLLFIFFSISFL--------KRTRNENPKSQNSLKLPPGSMGLPWIGETLQLYSQDP 71
           ++++++  F S++F+        +R +      + +++LPPGSMG P+IGETLQLYSQDP
Sbjct: 6   VIVIVISFFISLAFMCYVHYTSRQRRKLHGYGHEKAVRLPPGSMGWPYIGETLQLYSQDP 65

Query: 72  NIFFSQKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETMIGPAA 131
           N+FF+ KQ+RYGEIFKT+ILGCPCVMLAS  AARFVLVT AHLF+PTYP+SKE MIGP+A
Sbjct: 66  NVFFASKQKRYGEIFKTHILGCPCVMLASPEAARFVLVTQAHLFKPTYPRSKERMIGPSA 125

Query: 132 LFFHQGNYHSNLRKLVLNSLSLERLRTLVPCIEAAAISATDSWAAAGHVINTFLEMKKYS 191
           LFFHQG+YH  LRKLV   L  + LR LVP +EAA  S   SW   G+V +TF  MK+ S
Sbjct: 126 LFFHQGDYHLRLRKLVQGPLGPDALRALVPDVEAAVRSTLASW--DGNVSSTFHAMKRLS 185

Query: 192 FEVGIIAVF-GKLEDEYKEKLKQNYCILDKGYNCFPTRLPGTAYSKALSARKKLREILGE 251
           F+VGI+ +F G+L++  K +L+QNY I++KGYN FP   PGT Y KA+ AR++L  +L +
Sbjct: 186 FDVGIVTIFGGRLDERRKAELRQNYAIVEKGYNSFPNSFPGTLYYKAIQARRRLHGVLSD 245

Query: 252 IIMERREKRVTERDLVGHLLNFR-DENGESLSEDQIADNIIGVLFAAQDTTATVLTWILK 311
           I+ ERR +     DL+G L+  R  ++G  L+++Q+ADNIIGVLFAAQDTTA+VLTWI+K
Sbjct: 246 IMRERRARGEPGSDLLGCLMQSRAGDDGALLTDEQVADNIIGVLFAAQDTTASVLTWIVK 305

Query: 312 YLHDNFKLFEAVKAEQMEIHRRNGEGKMPLSWSQIKDMPFTHRVVLESLRMASIISFTFR 371
           YLHD+ KL EAV+AEQ  I   N  G++PL+W+Q + M  TH+V+LESLRMASIISFTFR
Sbjct: 306 YLHDHPKLLEAVRAEQAAIRAANDGGRLPLTWAQTRSMALTHKVILESLRMASIISFTFR 365

Query: 372 EAVVDVEYKGYLIPKGWKVMPLFRNIHHNHEYFPDPHIFDPSRFEVAPRANTFMPFGNGV 431
           EAV DVEYKG+LIPKGWKVMPLFRNIHHN +YF DP  FDPSRF+V+PR NTFMPFGNGV
Sbjct: 366 EAVADVEYKGFLIPKGWKVMPLFRNIHHNPDYFQDPQKFDPSRFKVSPRPNTFMPFGNGV 425

Query: 432 HSCPGNELAKLEILILLHHLITKFRWEVEGSQSGVEYGPFPMPVQGLPARFFKQTTTN 480
           H+CPGNELAKLE+L+L+HHL+T +RWE+ GS   VEY PFP+P  GL A+ ++  + +
Sbjct: 426 HACPGNELAKLEMLVLIHHLVTGYRWEIVGSSDEVEYSPFPVPKHGLLAKLWRDDSVS 481

[Close]

BLAST of CSPI07G07650 vs. ExPASy Swiss-Prot
Match: A2Z212 (Abscisic acid 8'-hydroxylase 3 OS=Oryza sativa subsp. indica OX=39946 GN=CYP707A7 PE=3 SV=1)

HSP 1 Score: 592.8 bits (1527), Expect = 3.5e-168
Identity = 287/478 (60.04%), Postives = 369/478 (77.20%), Query Frame = 0


Query: 12  ILLLLLFIFFSISFL--------KRTRNENPKSQNSLKLPPGSMGLPWIGETLQLYSQDP 71
           ++++++  F S++F+        +R +      + +++LPPGSMG P+IGETLQLYSQDP
Sbjct: 6   VIVIVISFFISLAFMCYVHYTSRQRRKLHGYGHEKAVRLPPGSMGWPYIGETLQLYSQDP 65

Query: 72  NIFFSQKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETMIGPAA 131
           N+FF+ KQ+RYGEIFKT+ILGCPCVMLAS  AARFVLVT AHLF+PTYP+SKE MIGP+A
Sbjct: 66  NVFFASKQKRYGEIFKTHILGCPCVMLASPEAARFVLVTQAHLFKPTYPRSKERMIGPSA 125

Query: 132 LFFHQGNYHSNLRKLVLNSLSLERLRTLVPCIEAAAISATDSWAAAGHVINTFLEMKKYS 191
           LFF+QG+YH  LRKLV   L  + LR LVP +EAA  S   SW   G+V +TF  MK+ S
Sbjct: 126 LFFNQGDYHLRLRKLVQGPLGPDALRALVPDVEAAVRSTLASW--DGNVSSTFHAMKRLS 185

Query: 192 FEVGIIAVF-GKLEDEYKEKLKQNYCILDKGYNCFPTRLPGTAYSKALSARKKLREILGE 251
           F+VGI+ +F G+L++  K +L+QNY I++KGYN FP   PGT Y KA+ AR++L  +L +
Sbjct: 186 FDVGIVTIFGGRLDERRKAELRQNYAIVEKGYNSFPNSFPGTLYYKAIQARRRLHGVLSD 245

Query: 252 IIMERREKRVTERDLVGHLLNFR-DENGESLSEDQIADNIIGVLFAAQDTTATVLTWILK 311
           I+ ERR +     DL+G L+  R  ++G  L+++Q+ADNIIGVLFAAQDTTA+VLTWI+K
Sbjct: 246 IMRERRARGEPGSDLLGCLMQSRAGDDGALLTDEQVADNIIGVLFAAQDTTASVLTWIVK 305

Query: 312 YLHDNFKLFEAVKAEQMEIHRRNGEGKMPLSWSQIKDMPFTHRVVLESLRMASIISFTFR 371
           YLHD+ KL EAV+AEQ  I   N  G++PL+W+Q + M  TH+V+LESLRMASIISFTFR
Sbjct: 306 YLHDHPKLLEAVRAEQAAIRAANDGGRLPLTWAQTRSMALTHKVILESLRMASIISFTFR 365

Query: 372 EAVVDVEYKGYLIPKGWKVMPLFRNIHHNHEYFPDPHIFDPSRFEVAPRANTFMPFGNGV 431
           EAV DVEYKG+LIPKGWKVMPLFRNIHHN +YF DP  FDPSRF+V+PR NTFMPFGNGV
Sbjct: 366 EAVADVEYKGFLIPKGWKVMPLFRNIHHNPDYFQDPQKFDPSRFKVSPRPNTFMPFGNGV 425

Query: 432 HSCPGNELAKLEILILLHHLITKFRWEVEGSQSGVEYGPFPMPVQGLPARFFKQTTTN 480
           H+CPGNELAKLE+L+L+HHL+T +RWE+ GS   VEY PFP+P  GL A+ ++  T +
Sbjct: 426 HACPGNELAKLEMLVLIHHLVTGYRWEIVGSSDEVEYSPFPVPKHGLLAKLWRDDTVS 481

[Close]

BLAST of CSPI07G07650 vs. ExPASy TrEMBL
Match: A0A1S3BZQ3 (abscisic acid 8'-hydroxylase 4 OS=Cucumis melo OX=3656 GN=LOC103495236 PE=3 SV=1)

HSP 1 Score: 941.4 bits (2432), Expect = 1.5e-270
Identity = 463/480 (96.46%), Postives = 469/480 (97.71%), Query Frame = 0


Query: 1   MEALSVPLYITILLLLLFIFFSISFLKRTRNENPKSQNSLKLPPGSMGLPWIGETLQLYS 60
           MEALSVPLYITIL L LF FFSISFLKR+RNEN KSQNSLKLPPGSMGLPWIGETLQLYS
Sbjct: 1   MEALSVPLYITILFLFLFTFFSISFLKRSRNENSKSQNSLKLPPGSMGLPWIGETLQLYS 60

Query: 61  QDPNIFFSQKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETMIG 120
           QDPNIFFSQKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETMIG
Sbjct: 61  QDPNIFFSQKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETMIG 120

Query: 121 PAALFFHQGNYHSNLRKLVLNSLSLERLRTLVPCIEAAAISATDSWAAAGHVINTFLEMK 180
           PAALFFHQGNYHSNLRKLVLNSLSLERLRTLVP IEAAAISATDSWAAAGHVINTF EMK
Sbjct: 121 PAALFFHQGNYHSNLRKLVLNSLSLERLRTLVPSIEAAAISATDSWAAAGHVINTFREMK 180

Query: 181 KYSFEVGIIAVFGKLEDEYKEKLKQNYCILDKGYNCFPTRLPGTAYSKALSARKKLREIL 240
           KYSFEVGIIAVFGKLE+EYKEKLKQ YCILDKGYNCFPTRLPGT YSKALSARKKLREIL
Sbjct: 181 KYSFEVGIIAVFGKLENEYKEKLKQKYCILDKGYNCFPTRLPGTGYSKALSARKKLREIL 240

Query: 241 GEIIMERREKRVTERDLVGHLLNFRDENGESLSEDQIADNIIGVLFAAQDTTATVLTWIL 300
           GEIIMERREKRVTERD VGHLLN+RDENGESLSEDQIADNIIGVLFAAQDTTATVLTWIL
Sbjct: 241 GEIIMERREKRVTERDFVGHLLNYRDENGESLSEDQIADNIIGVLFAAQDTTATVLTWIL 300

Query: 301 KYLHDNFKLFEAVKAEQMEIHRRNGEGKMPLSWSQIKDMPFTHRVVLESLRMASIISFTF 360
           KYLHDNFKLFEAVKAEQMEI+RRNGEGKMPLSW QIKDMPFTHRVVLESLRMASIISFTF
Sbjct: 301 KYLHDNFKLFEAVKAEQMEIYRRNGEGKMPLSWGQIKDMPFTHRVVLESLRMASIISFTF 360

Query: 361 REAVVDVEYKGYLIPKGWKVMPLFRNIHHNHEYFPDPHIFDPSRFEVAPRANTFMPFGNG 420
           REAVVDVEYKGYLIPKGWKVMPLFRNIHHNHEYFPDPHIFDPSRFEVAPR NTFMPFGNG
Sbjct: 361 REAVVDVEYKGYLIPKGWKVMPLFRNIHHNHEYFPDPHIFDPSRFEVAPRPNTFMPFGNG 420

Query: 421 VHSCPGNELAKLEILILLHHLITKFRWEVEGSQSGVEYGPFPMPVQGLPARFFKQTTTNQ 480
           VHSCPGNELAKLEILILLHHLITKFRWEVEGSQSGVEYGPFPMP+QGLP+RFFKQTTTNQ
Sbjct: 421 VHSCPGNELAKLEILILLHHLITKFRWEVEGSQSGVEYGPFPMPLQGLPSRFFKQTTTNQ 480

[Close]

BLAST of CSPI07G07650 vs. ExPASy TrEMBL
Match: A0A0A0K6K8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G073680 PE=3 SV=1)

HSP 1 Score: 898.7 bits (2321), Expect = 1.1e-257
Identity = 437/439 (99.54%), Postives = 439/439 (100.00%), Query Frame = 0


Query: 47  MGLPWIGETLQLYSQDPNIFFSQKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHL 106
           MGLPWIGETLQLYSQDPNIFFSQKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHL
Sbjct: 1   MGLPWIGETLQLYSQDPNIFFSQKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHL 60

Query: 107 FRPTYPKSKETMIGPAALFFHQGNYHSNLRKLVLNSLSLERLRTLVPCIEAAAISATDSW 166
           FRPTYPKSKETMIGPAALFFHQGNYHSNLRKLVLNSLSLERLRTLVPCIEAAAISATDSW
Sbjct: 61  FRPTYPKSKETMIGPAALFFHQGNYHSNLRKLVLNSLSLERLRTLVPCIEAAAISATDSW 120

Query: 167 AAAGHVINTFLEMKKYSFEVGIIAVFGKLEDEYKEKLKQNYCILDKGYNCFPTRLPGTAY 226
           AAAGHVINTFLEMKKYSFEVGIIAVFGKLEDEYKEKLKQNYCILDKGYNCFPTRLPGTAY
Sbjct: 121 AAAGHVINTFLEMKKYSFEVGIIAVFGKLEDEYKEKLKQNYCILDKGYNCFPTRLPGTAY 180

Query: 227 SKALSARKKLREILGEIIMERREKRVTERDLVGHLLNFRDENGESLSEDQIADNIIGVLF 286
           SKALSARKKLREILGEIIMERREKRVTERDLVGHLLNFRDENGE+LSEDQIADNIIGVLF
Sbjct: 181 SKALSARKKLREILGEIIMERREKRVTERDLVGHLLNFRDENGENLSEDQIADNIIGVLF 240

Query: 287 AAQDTTATVLTWILKYLHDNFKLFEAVKAEQMEIHRRNGEGKMPLSWSQIKDMPFTHRVV 346
           AAQDTTATVLTWILKYLHDNFKLFEAVKAEQMEI+RRNGEGKMPLSWSQIKDMPFTHRVV
Sbjct: 241 AAQDTTATVLTWILKYLHDNFKLFEAVKAEQMEIYRRNGEGKMPLSWSQIKDMPFTHRVV 300

Query: 347 LESLRMASIISFTFREAVVDVEYKGYLIPKGWKVMPLFRNIHHNHEYFPDPHIFDPSRFE 406
           LESLRMASIISFTFREAVVDVEYKGYLIPKGWKVMPLFRNIHHNHEYFPDPHIFDPSRFE
Sbjct: 301 LESLRMASIISFTFREAVVDVEYKGYLIPKGWKVMPLFRNIHHNHEYFPDPHIFDPSRFE 360

Query: 407 VAPRANTFMPFGNGVHSCPGNELAKLEILILLHHLITKFRWEVEGSQSGVEYGPFPMPVQ 466
           VAPRANTFMPFGNGVHSCPGNELAKLEILILLHHLITKFRWEVEGSQSGVEYGPFPMPVQ
Sbjct: 361 VAPRANTFMPFGNGVHSCPGNELAKLEILILLHHLITKFRWEVEGSQSGVEYGPFPMPVQ 420

Query: 467 GLPARFFKQTTTNQYPFLS 486
           GLPARFFKQTTTNQYPFLS
Sbjct: 421 GLPARFFKQTTTNQYPFLS 439

[Close]

BLAST of CSPI07G07650 vs. ExPASy TrEMBL
Match: A0A5A7SMJ8 (Abscisic acid 8'-hydroxylase 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G00490 PE=3 SV=1)

HSP 1 Score: 868.6 bits (2243), Expect = 1.2e-248
Identity = 422/434 (97.24%), Postives = 427/434 (98.39%), Query Frame = 0


Query: 47  MGLPWIGETLQLYSQDPNIFFSQKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHL 106
           MGLPWIGETLQLYSQDPNIFFSQKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHL
Sbjct: 1   MGLPWIGETLQLYSQDPNIFFSQKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHL 60

Query: 107 FRPTYPKSKETMIGPAALFFHQGNYHSNLRKLVLNSLSLERLRTLVPCIEAAAISATDSW 166
           FRPTYPKSKETMIGPAALFFHQGNYHSNLRKLVLNSLSLERLRTLVP IEAAAISATDSW
Sbjct: 61  FRPTYPKSKETMIGPAALFFHQGNYHSNLRKLVLNSLSLERLRTLVPSIEAAAISATDSW 120

Query: 167 AAAGHVINTFLEMKKYSFEVGIIAVFGKLEDEYKEKLKQNYCILDKGYNCFPTRLPGTAY 226
           AAAGHVINTF EMKKYSFEVGIIAVFGKLE+EYKEKLKQ YCILDKGYNCFPTRLPGT Y
Sbjct: 121 AAAGHVINTFREMKKYSFEVGIIAVFGKLENEYKEKLKQKYCILDKGYNCFPTRLPGTGY 180

Query: 227 SKALSARKKLREILGEIIMERREKRVTERDLVGHLLNFRDENGESLSEDQIADNIIGVLF 286
           SKALSARKKLREILGEIIMERREKRVTERD VGHLLN+RDENGESLSEDQIADNIIGVLF
Sbjct: 181 SKALSARKKLREILGEIIMERREKRVTERDFVGHLLNYRDENGESLSEDQIADNIIGVLF 240

Query: 287 AAQDTTATVLTWILKYLHDNFKLFEAVKAEQMEIHRRNGEGKMPLSWSQIKDMPFTHRVV 346
           AAQDTTATVLTWILKYLHDNFKLFEAVKAEQMEI+RRNGEGKMPLSW QIKDMPFTHRVV
Sbjct: 241 AAQDTTATVLTWILKYLHDNFKLFEAVKAEQMEIYRRNGEGKMPLSWGQIKDMPFTHRVV 300

Query: 347 LESLRMASIISFTFREAVVDVEYKGYLIPKGWKVMPLFRNIHHNHEYFPDPHIFDPSRFE 406
           LESLRMASIISFTFREAVVDVEYKGYLIPKGWKVMPLFRNIHHNHEYFPDPHIFDPSRFE
Sbjct: 301 LESLRMASIISFTFREAVVDVEYKGYLIPKGWKVMPLFRNIHHNHEYFPDPHIFDPSRFE 360

Query: 407 VAPRANTFMPFGNGVHSCPGNELAKLEILILLHHLITKFRWEVEGSQSGVEYGPFPMPVQ 466
           VAPR NTFMPFGNGVHSCPGNELAKLEILILLHHLITKFRWEVEGSQSGVEYGPFPMP+Q
Sbjct: 361 VAPRPNTFMPFGNGVHSCPGNELAKLEILILLHHLITKFRWEVEGSQSGVEYGPFPMPLQ 420

Query: 467 GLPARFFKQTTTNQ 481
           GLP+RFFKQTTTNQ
Sbjct: 421 GLPSRFFKQTTTNQ 434

[Close]

BLAST of CSPI07G07650 vs. ExPASy TrEMBL
Match: A0A6J1I8M8 (abscisic acid 8'-hydroxylase 4-like OS=Cucurbita maxima OX=3661 GN=LOC111471004 PE=3 SV=1)

HSP 1 Score: 848.6 bits (2191), Expect = 1.3e-242
Identity = 421/481 (87.53%), Postives = 445/481 (92.52%), Query Frame = 0


Query: 1   MEALSVPLYITILLLLLFIFFSISFLKRTRNENPKSQNSLKLPPGSMGLPWIGETLQLYS 60
           MEALSVPLYI I L LL  FFSISFLK  RNE PKSQ +LKLPPGSMGLPW GETLQLYS
Sbjct: 1   MEALSVPLYIIISLFLL-AFFSISFLK--RNEQPKSQEALKLPPGSMGLPWFGETLQLYS 60

Query: 61  QDPNIFFSQKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETMIG 120
           QDPNIFFS+KQRR+GEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKET+IG
Sbjct: 61  QDPNIFFSEKQRRFGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETLIG 120

Query: 121 PAALFFHQGNYHSNLRKLVLNSLSLERLR-TLVPCIEAAAISATDSWAAAGHVINTFLEM 180
           PAALFFHQGNYHSNLRKLV NSLSL+RLR TLVP IEAAAISA DSWAA  HV+NTFL+M
Sbjct: 121 PAALFFHQGNYHSNLRKLVQNSLSLQRLRTTLVPHIEAAAISAADSWAATAHVVNTFLQM 180

Query: 181 KKYSFEVGIIAVFGKLEDEYKEKLKQNYCILDKGYNCFPTRLPGTAYSKALSARKKLREI 240
           KK+SFEV I+AVFGKLE  Y EKLK+NYCILDKGYN FPTRLPGTA SKALSARKKLREI
Sbjct: 181 KKFSFEVAIVAVFGKLETHYSEKLKENYCILDKGYNSFPTRLPGTACSKALSARKKLREI 240

Query: 241 LGEIIMERREKRVTERDLVGHLLNFRDENGESLSEDQIADNIIGVLFAAQDTTATVLTWI 300
           L EII+ERREK   ERD VG LL++RDENGE LSE+QIADN+IGVLFAAQDTTA+VLTWI
Sbjct: 241 LREIIVERREKEEAERDFVGQLLSYRDENGERLSEEQIADNVIGVLFAAQDTTASVLTWI 300

Query: 301 LKYLHDNFKLFEAVKAEQMEIHRRNGEGKMPLSWSQIKDMPFTHRVVLESLRMASIISFT 360
           LKYLHD+FKLFEAV+AEQMEI+RRNGEGKMPLSW+QIKDMPFTHRVVLESLRMASIISFT
Sbjct: 301 LKYLHDSFKLFEAVRAEQMEIYRRNGEGKMPLSWAQIKDMPFTHRVVLESLRMASIISFT 360

Query: 361 FREAVVDVEYKGYLIPKGWKVMPLFRNIHHNHEYFPDPHIFDPSRFEVAPRANTFMPFGN 420
           FREAVVDVEYKGY IPKGWKVMPLFRNIHHNH+YF DPH FDPSRFEVAPR NTFMPFGN
Sbjct: 361 FREAVVDVEYKGYRIPKGWKVMPLFRNIHHNHDYFNDPHTFDPSRFEVAPRPNTFMPFGN 420

Query: 421 GVHSCPGNELAKLEILILLHHLITKFRWEVEGSQSGVEYGPFPMPVQGLPARFFKQTTTN 480
           GVHSCPGNELAKLEILILLHHL+TKFRWEV G QSGV+YGPFP+PV GLPARFFKQTTTN
Sbjct: 421 GVHSCPGNELAKLEILILLHHLVTKFRWEVVGPQSGVQYGPFPVPVHGLPARFFKQTTTN 478

[Close]

BLAST of CSPI07G07650 vs. ExPASy TrEMBL
Match: A0A6J1EHU3 (abscisic acid 8'-hydroxylase 4-like OS=Cucurbita moschata OX=3662 GN=LOC111434488 PE=3 SV=1)

HSP 1 Score: 847.0 bits (2187), Expect = 3.8e-242
Identity = 417/480 (86.88%), Postives = 440/480 (91.67%), Query Frame = 0


Query: 1   MEALSVPLYITILLLLLFIFFSISFLKRTRNENPKSQNSLKLPPGSMGLPWIGETLQLYS 60
           ME LS  LY     L++F   S   L   RNE PK + SL+LPPGSMGLPWIGETLQLYS
Sbjct: 1   MEILSFTLY-----LIIFFISSAFLLYFKRNETPKPRKSLRLPPGSMGLPWIGETLQLYS 60

Query: 61  QDPNIFFSQKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETMIG 120
           QDPNIFFS+KQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETMIG
Sbjct: 61  QDPNIFFSEKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETMIG 120

Query: 121 PAALFFHQGNYHSNLRKLVLNSLSLERLRTLVPCIEAAAISATDSWAAAGHVINTFLEMK 180
           PAALFFHQGNYHSN+RKLV NSL L  LR LVP IE AAISA +SWAAAGHVINTFLEMK
Sbjct: 121 PAALFFHQGNYHSNIRKLVQNSLCLGSLRNLVPHIETAAISAAESWAAAGHVINTFLEMK 180

Query: 181 KYSFEVGIIAVFGKLEDEYKEKLKQNYCILDKGYNCFPTRLPGTAYSKALSARKKLREIL 240
           K+SF+VGI+AVFGKLE +Y EKLK+NYCILDKGYN FPTRLPGTAYSKALSARKKLREIL
Sbjct: 181 KFSFDVGILAVFGKLESDYSEKLKENYCILDKGYNSFPTRLPGTAYSKALSARKKLREIL 240

Query: 241 GEIIMERREKRVTERDLVGHLLNFRDENGESLSEDQIADNIIGVLFAAQDTTATVLTWIL 300
           GEIIMERREKRVTERDLVGHLL+FRDENG  LSEDQIADNIIGVLFAAQDTTA+VLTWIL
Sbjct: 241 GEIIMERREKRVTERDLVGHLLSFRDENGGILSEDQIADNIIGVLFAAQDTTASVLTWIL 300

Query: 301 KYLHDNFKLFEAVKAEQMEIHRRNGEGKMPLSWSQIKDMPFTHRVVLESLRMASIISFTF 360
           KYLHDN KLFEAV+AEQMEI+RRNG+GKMPLSW+QIKDMP THRVVLESLRMASIISFTF
Sbjct: 301 KYLHDNLKLFEAVRAEQMEIYRRNGDGKMPLSWAQIKDMPLTHRVVLESLRMASIISFTF 360

Query: 361 REAVVDVEYKGYLIPKGWKVMPLFRNIHHNHEYFPDPHIFDPSRFEVAPRANTFMPFGNG 420
           REAVVDVEYKG+LIPKGWKVMPLFRNIHHN +YFP+P IFDPSRFEVAPR NTFMPFGNG
Sbjct: 361 REAVVDVEYKGFLIPKGWKVMPLFRNIHHNPDYFPNPQIFDPSRFEVAPRPNTFMPFGNG 420

Query: 421 VHSCPGNELAKLEILILLHHLITKFRWEVEGSQSGVEYGPFPMPVQGLPARFFKQTTTNQ 480
           VHSCPGNELAKLEIL+LLHHLITKFRWEV GSQSGV+YGPFPMPV GLPARFFKQTTTNQ
Sbjct: 421 VHSCPGNELAKLEILVLLHHLITKFRWEVVGSQSGVQYGPFPMPVNGLPARFFKQTTTNQ 475

[Close]

BLAST of CSPI07G07650 vs. NCBI nr
Match: XP_004137112.1 (abscisic acid 8'-hydroxylase 4 [Cucumis sativus] >KAE8645865.1 hypothetical protein Csa_017084 [Cucumis sativus])

HSP 1 Score: 981.1 bits (2535), Expect = 3.5e-282
Identity = 483/485 (99.59%), Postives = 485/485 (100.00%), Query Frame = 0


Query: 1   MEALSVPLYITILLLLLFIFFSISFLKRTRNENPKSQNSLKLPPGSMGLPWIGETLQLYS 60
           MEALSVPLYITILLLLLFIFFSISFLKRTRNENPKSQNSLKLPPGSMGLPWIGETLQLYS
Sbjct: 1   MEALSVPLYITILLLLLFIFFSISFLKRTRNENPKSQNSLKLPPGSMGLPWIGETLQLYS 60

Query: 61  QDPNIFFSQKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETMIG 120
           QDPNIFFSQKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETMIG
Sbjct: 61  QDPNIFFSQKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETMIG 120

Query: 121 PAALFFHQGNYHSNLRKLVLNSLSLERLRTLVPCIEAAAISATDSWAAAGHVINTFLEMK 180
           PAALFFHQGNYHSNLRKLVLNSLSLERLRTLVPCIEAAAISATDSWAAAGHVINTFLEMK
Sbjct: 121 PAALFFHQGNYHSNLRKLVLNSLSLERLRTLVPCIEAAAISATDSWAAAGHVINTFLEMK 180

Query: 181 KYSFEVGIIAVFGKLEDEYKEKLKQNYCILDKGYNCFPTRLPGTAYSKALSARKKLREIL 240
           KYSFEVGIIAVFGKLEDEYKEKLKQNYCILDKGYNCFPTRLPGTAYSKALSARKKLREIL
Sbjct: 181 KYSFEVGIIAVFGKLEDEYKEKLKQNYCILDKGYNCFPTRLPGTAYSKALSARKKLREIL 240

Query: 241 GEIIMERREKRVTERDLVGHLLNFRDENGESLSEDQIADNIIGVLFAAQDTTATVLTWIL 300
           GEIIMERREKRVTERDLVGHLLNFRDENGE+LSEDQIADNIIGVLFAAQDTTATVLTWIL
Sbjct: 241 GEIIMERREKRVTERDLVGHLLNFRDENGENLSEDQIADNIIGVLFAAQDTTATVLTWIL 300

Query: 301 KYLHDNFKLFEAVKAEQMEIHRRNGEGKMPLSWSQIKDMPFTHRVVLESLRMASIISFTF 360
           KYLHDNFKLFEAVKAEQMEI+RRNGEGKMPLSWSQIKDMPFTHRVVLESLRMASIISFTF
Sbjct: 301 KYLHDNFKLFEAVKAEQMEIYRRNGEGKMPLSWSQIKDMPFTHRVVLESLRMASIISFTF 360

Query: 361 REAVVDVEYKGYLIPKGWKVMPLFRNIHHNHEYFPDPHIFDPSRFEVAPRANTFMPFGNG 420
           REAVVDVEYKGYLIPKGWKVMPLFRNIHHNHEYFPDPHIFDPSRFEVAPRANTFMPFGNG
Sbjct: 361 REAVVDVEYKGYLIPKGWKVMPLFRNIHHNHEYFPDPHIFDPSRFEVAPRANTFMPFGNG 420

Query: 421 VHSCPGNELAKLEILILLHHLITKFRWEVEGSQSGVEYGPFPMPVQGLPARFFKQTTTNQ 480
           VHSCPGNELAKLEILILLHHLITKFRWEVEGSQSGVEYGPFPMPVQGLPARFFKQTTTNQ
Sbjct: 421 VHSCPGNELAKLEILILLHHLITKFRWEVEGSQSGVEYGPFPMPVQGLPARFFKQTTTNQ 480

Query: 481 YPFLS 486
           YPFLS
Sbjct: 481 YPFLS 485

[Close]

BLAST of CSPI07G07650 vs. NCBI nr
Match: XP_008454947.1 (PREDICTED: abscisic acid 8'-hydroxylase 4 [Cucumis melo])

HSP 1 Score: 941.4 bits (2432), Expect = 3.1e-270
Identity = 463/480 (96.46%), Postives = 469/480 (97.71%), Query Frame = 0


Query: 1   MEALSVPLYITILLLLLFIFFSISFLKRTRNENPKSQNSLKLPPGSMGLPWIGETLQLYS 60
           MEALSVPLYITIL L LF FFSISFLKR+RNEN KSQNSLKLPPGSMGLPWIGETLQLYS
Sbjct: 1   MEALSVPLYITILFLFLFTFFSISFLKRSRNENSKSQNSLKLPPGSMGLPWIGETLQLYS 60

Query: 61  QDPNIFFSQKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETMIG 120
           QDPNIFFSQKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETMIG
Sbjct: 61  QDPNIFFSQKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETMIG 120

Query: 121 PAALFFHQGNYHSNLRKLVLNSLSLERLRTLVPCIEAAAISATDSWAAAGHVINTFLEMK 180
           PAALFFHQGNYHSNLRKLVLNSLSLERLRTLVP IEAAAISATDSWAAAGHVINTF EMK
Sbjct: 121 PAALFFHQGNYHSNLRKLVLNSLSLERLRTLVPSIEAAAISATDSWAAAGHVINTFREMK 180

Query: 181 KYSFEVGIIAVFGKLEDEYKEKLKQNYCILDKGYNCFPTRLPGTAYSKALSARKKLREIL 240
           KYSFEVGIIAVFGKLE+EYKEKLKQ YCILDKGYNCFPTRLPGT YSKALSARKKLREIL
Sbjct: 181 KYSFEVGIIAVFGKLENEYKEKLKQKYCILDKGYNCFPTRLPGTGYSKALSARKKLREIL 240

Query: 241 GEIIMERREKRVTERDLVGHLLNFRDENGESLSEDQIADNIIGVLFAAQDTTATVLTWIL 300
           GEIIMERREKRVTERD VGHLLN+RDENGESLSEDQIADNIIGVLFAAQDTTATVLTWIL
Sbjct: 241 GEIIMERREKRVTERDFVGHLLNYRDENGESLSEDQIADNIIGVLFAAQDTTATVLTWIL 300

Query: 301 KYLHDNFKLFEAVKAEQMEIHRRNGEGKMPLSWSQIKDMPFTHRVVLESLRMASIISFTF 360
           KYLHDNFKLFEAVKAEQMEI+RRNGEGKMPLSW QIKDMPFTHRVVLESLRMASIISFTF
Sbjct: 301 KYLHDNFKLFEAVKAEQMEIYRRNGEGKMPLSWGQIKDMPFTHRVVLESLRMASIISFTF 360

Query: 361 REAVVDVEYKGYLIPKGWKVMPLFRNIHHNHEYFPDPHIFDPSRFEVAPRANTFMPFGNG 420
           REAVVDVEYKGYLIPKGWKVMPLFRNIHHNHEYFPDPHIFDPSRFEVAPR NTFMPFGNG
Sbjct: 361 REAVVDVEYKGYLIPKGWKVMPLFRNIHHNHEYFPDPHIFDPSRFEVAPRPNTFMPFGNG 420

Query: 421 VHSCPGNELAKLEILILLHHLITKFRWEVEGSQSGVEYGPFPMPVQGLPARFFKQTTTNQ 480
           VHSCPGNELAKLEILILLHHLITKFRWEVEGSQSGVEYGPFPMP+QGLP+RFFKQTTTNQ
Sbjct: 421 VHSCPGNELAKLEILILLHHLITKFRWEVEGSQSGVEYGPFPMPLQGLPSRFFKQTTTNQ 480

[Close]

BLAST of CSPI07G07650 vs. NCBI nr
Match: XP_038887333.1 (abscisic acid 8'-hydroxylase 4 [Benincasa hispida])

HSP 1 Score: 887.1 bits (2291), Expect = 6.8e-254
Identity = 442/484 (91.32%), Postives = 460/484 (95.04%), Query Frame = 0


Query: 1   MEALSVPLYITILLLL-LFI---FFSISFLKRTRNENPKSQNSLKLPPGSMGLPWIGETL 60
           MEALSV LYI I++++ LF+   FFSISFLK  RN+ PKSQ  LKLPPGSMGLPWIGETL
Sbjct: 1   MEALSVTLYIIIIIIIFLFLFAFFFSISFLK--RNQKPKSQKVLKLPPGSMGLPWIGETL 60

Query: 61  QLYSQDPNIFFSQKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKE 120
           QLYSQDPNIFFSQKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKE
Sbjct: 61  QLYSQDPNIFFSQKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKE 120

Query: 121 TMIGPAALFFHQGNYHSNLRKLVLNSLSLERLRTLVPCIEAAAISATDSWAAAGHVINTF 180
           TMIGPAALFFHQGNYHSNLRKLV NSLSLERLR LVP IEAAAISATDSWAAAGHVINTF
Sbjct: 121 TMIGPAALFFHQGNYHSNLRKLVQNSLSLERLRILVPHIEAAAISATDSWAAAGHVINTF 180

Query: 181 LEMKKYSFEVGIIAVFGKLEDEYKEKLKQNYCILDKGYNCFPTRLPGTAYSKALSARKKL 240
           LEMKKYSFEVGI+AVF KLE+EYKEKLK+ Y ILDKGYNCFPTRLPGTAYSKALSARKKL
Sbjct: 181 LEMKKYSFEVGIVAVFEKLENEYKEKLKEKYSILDKGYNCFPTRLPGTAYSKALSARKKL 240

Query: 241 REILGEIIMERREKRVTERDLVGHLLNFRDENGESLSEDQIADNIIGVLFAAQDTTATVL 300
           REILGEIIMERREKRVTERDLVGHLLNFRDENGE+LSEDQIADNIIGVLFAAQDTTAT L
Sbjct: 241 REILGEIIMERREKRVTERDLVGHLLNFRDENGETLSEDQIADNIIGVLFAAQDTTATAL 300

Query: 301 TWILKYLHDNFKLFEAVKAEQMEIHRRNGEGKMPLSWSQIKDMPFTHRVVLESLRMASII 360
           TWILK+LHDN KLFEAVKAEQMEI+RRNG+GKMPLSW+QIKDMPFTHRVVLESLRMASII
Sbjct: 301 TWILKFLHDNLKLFEAVKAEQMEIYRRNGDGKMPLSWAQIKDMPFTHRVVLESLRMASII 360

Query: 361 SFTFREAVVDVEYKGYLIPKGWKVMPLFRNIHHNHEYFPDPHIFDPSRFEVAPRANTFMP 420
           SFTFREAVVD+EYKGYLIPKGWKVMPLFRNIHHN EYFPDPHIFDPSRFE+AP+ NTFMP
Sbjct: 361 SFTFREAVVDMEYKGYLIPKGWKVMPLFRNIHHNPEYFPDPHIFDPSRFELAPKPNTFMP 420

Query: 421 FGNGVHSCPGNELAKLEILILLHHLITKFRWEVEGSQSGVEYGPFPMPVQGLPARFFKQT 480
           FGNGVHSCPGNELAKLEILILLHHLITKFRWEV GS+SGVEYGPFPMPV GLPARFFK T
Sbjct: 421 FGNGVHSCPGNELAKLEILILLHHLITKFRWEVVGSESGVEYGPFPMPVHGLPARFFKHT 480

[Close]

BLAST of CSPI07G07650 vs. NCBI nr
Match: KAA0031296.1 (abscisic acid 8'-hydroxylase 4 [Cucumis melo var. makuwa] >TYK06748.1 abscisic acid 8'-hydroxylase 4 [Cucumis melo var. makuwa])

HSP 1 Score: 868.6 bits (2243), Expect = 2.5e-248
Identity = 422/434 (97.24%), Postives = 427/434 (98.39%), Query Frame = 0


Query: 47  MGLPWIGETLQLYSQDPNIFFSQKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHL 106
           MGLPWIGETLQLYSQDPNIFFSQKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHL
Sbjct: 1   MGLPWIGETLQLYSQDPNIFFSQKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHL 60

Query: 107 FRPTYPKSKETMIGPAALFFHQGNYHSNLRKLVLNSLSLERLRTLVPCIEAAAISATDSW 166
           FRPTYPKSKETMIGPAALFFHQGNYHSNLRKLVLNSLSLERLRTLVP IEAAAISATDSW
Sbjct: 61  FRPTYPKSKETMIGPAALFFHQGNYHSNLRKLVLNSLSLERLRTLVPSIEAAAISATDSW 120

Query: 167 AAAGHVINTFLEMKKYSFEVGIIAVFGKLEDEYKEKLKQNYCILDKGYNCFPTRLPGTAY 226
           AAAGHVINTF EMKKYSFEVGIIAVFGKLE+EYKEKLKQ YCILDKGYNCFPTRLPGT Y
Sbjct: 121 AAAGHVINTFREMKKYSFEVGIIAVFGKLENEYKEKLKQKYCILDKGYNCFPTRLPGTGY 180

Query: 227 SKALSARKKLREILGEIIMERREKRVTERDLVGHLLNFRDENGESLSEDQIADNIIGVLF 286
           SKALSARKKLREILGEIIMERREKRVTERD VGHLLN+RDENGESLSEDQIADNIIGVLF
Sbjct: 181 SKALSARKKLREILGEIIMERREKRVTERDFVGHLLNYRDENGESLSEDQIADNIIGVLF 240

Query: 287 AAQDTTATVLTWILKYLHDNFKLFEAVKAEQMEIHRRNGEGKMPLSWSQIKDMPFTHRVV 346
           AAQDTTATVLTWILKYLHDNFKLFEAVKAEQMEI+RRNGEGKMPLSW QIKDMPFTHRVV
Sbjct: 241 AAQDTTATVLTWILKYLHDNFKLFEAVKAEQMEIYRRNGEGKMPLSWGQIKDMPFTHRVV 300

Query: 347 LESLRMASIISFTFREAVVDVEYKGYLIPKGWKVMPLFRNIHHNHEYFPDPHIFDPSRFE 406
           LESLRMASIISFTFREAVVDVEYKGYLIPKGWKVMPLFRNIHHNHEYFPDPHIFDPSRFE
Sbjct: 301 LESLRMASIISFTFREAVVDVEYKGYLIPKGWKVMPLFRNIHHNHEYFPDPHIFDPSRFE 360

Query: 407 VAPRANTFMPFGNGVHSCPGNELAKLEILILLHHLITKFRWEVEGSQSGVEYGPFPMPVQ 466
           VAPR NTFMPFGNGVHSCPGNELAKLEILILLHHLITKFRWEVEGSQSGVEYGPFPMP+Q
Sbjct: 361 VAPRPNTFMPFGNGVHSCPGNELAKLEILILLHHLITKFRWEVEGSQSGVEYGPFPMPLQ 420

Query: 467 GLPARFFKQTTTNQ 481
           GLP+RFFKQTTTNQ
Sbjct: 421 GLPSRFFKQTTTNQ 434

[Close]

BLAST of CSPI07G07650 vs. NCBI nr
Match: XP_023553809.1 (abscisic acid 8'-hydroxylase 4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 852.0 bits (2200), Expect = 2.4e-243
Identity = 421/481 (87.53%), Postives = 446/481 (92.72%), Query Frame = 0


Query: 1   MEALSVPLYITILLLLLFIFFSISFLKRTRNENPKSQNSLKLPPGSMGLPWIGETLQLYS 60
           MEALSVPLYI I L LL  FFSISFLK  RNE PK+Q +LKLPPGSMGLPW GETLQLYS
Sbjct: 1   MEALSVPLYIIISLFLL-AFFSISFLK--RNEQPKAQEALKLPPGSMGLPWFGETLQLYS 60

Query: 61  QDPNIFFSQKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETMIG 120
           QDPNIFFS+KQRR+GEIFKTNILGCPCVMLASAAAARFV VTNAHLFRPTYPKSKET+IG
Sbjct: 61  QDPNIFFSEKQRRFGEIFKTNILGCPCVMLASAAAARFVFVTNAHLFRPTYPKSKETLIG 120

Query: 121 PAALFFHQGNYHSNLRKLVLNSLSLERLR-TLVPCIEAAAISATDSWAAAGHVINTFLEM 180
           PAALFFHQGNYHSNLRKLV NSL LERLR TLVP IEAAAISA DSWAA GHV+NTFL+M
Sbjct: 121 PAALFFHQGNYHSNLRKLVQNSLCLERLRTTLVPHIEAAAISAADSWAATGHVVNTFLQM 180

Query: 181 KKYSFEVGIIAVFGKLEDEYKEKLKQNYCILDKGYNCFPTRLPGTAYSKALSARKKLREI 240
           KK+SFEV I+AVFGKLE +Y EKLK+NYCILDKGYN FPTRLPGTAYSKALSARKKLREI
Sbjct: 181 KKFSFEVAIVAVFGKLETQYSEKLKENYCILDKGYNSFPTRLPGTAYSKALSARKKLREI 240

Query: 241 LGEIIMERREKRVTERDLVGHLLNFRDENGESLSEDQIADNIIGVLFAAQDTTATVLTWI 300
           L EII+ERREK   ERD VG LL++RDENGE LSE+QIADN+IGVLFAAQDTTA+VLTWI
Sbjct: 241 LREIIVERREKGAAERDFVGQLLSYRDENGEGLSEEQIADNVIGVLFAAQDTTASVLTWI 300

Query: 301 LKYLHDNFKLFEAVKAEQMEIHRRNGEGKMPLSWSQIKDMPFTHRVVLESLRMASIISFT 360
           LKYLHD+ KLFEAV+AEQMEI+RRNGEGKMPLSW+QIKDMPFTHRVVLESLRMASIISFT
Sbjct: 301 LKYLHDSLKLFEAVRAEQMEIYRRNGEGKMPLSWAQIKDMPFTHRVVLESLRMASIISFT 360

Query: 361 FREAVVDVEYKGYLIPKGWKVMPLFRNIHHNHEYFPDPHIFDPSRFEVAPRANTFMPFGN 420
           FREAVVDVEYKGYLIPKGWKVMPLFRNIHHNH+YF DPH FDPSRFEVAPR NTFMPFGN
Sbjct: 361 FREAVVDVEYKGYLIPKGWKVMPLFRNIHHNHDYFNDPHTFDPSRFEVAPRPNTFMPFGN 420

Query: 421 GVHSCPGNELAKLEILILLHHLITKFRWEVEGSQSGVEYGPFPMPVQGLPARFFKQTTTN 480
           GVHSCPGNELAKLEILILLHHL+TKFRWEV G QSGV+YGPFP+PV GLPARFFKQTTTN
Sbjct: 421 GVHSCPGNELAKLEILILLHHLVTKFRWEVVGPQSGVQYGPFPVPVHGLPARFFKQTTTN 478

[Close]

BLAST of CSPI07G07650 vs. TAIR 10
Match: AT3G19270.1 (cytochrome P450, family 707, subfamily A, polypeptide 4 )

HSP 1 Score: 632.9 bits (1631), Expect = 2.2e-181
Identity = 308/470 (65.53%), Postives = 376/470 (80.00%), Query Frame = 0


Query: 4   LSVPLYITILLLLLFIFFSISFLKRTRNENPKSQNSL-KLPPGSMGLPWIGETLQLYSQD 63
           L VP+ I  LLL+  I             + K +NS  KLPPGSMG P++GETLQLYSQ+
Sbjct: 7   LVVPILILCLLLVRVIV------------SKKKKNSRGKLPPGSMGWPYLGETLQLYSQN 66

Query: 64  PNIFFSQKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETMIGPA 123
           PN+FF+ KQ+RYGEIFKT ILG PCVMLAS  AARFVLVT+AH+F+PTYP+SKE +IGP+
Sbjct: 67  PNVFFTSKQKRYGEIFKTRILGYPCVMLASPEAARFVLVTHAHMFKPTYPRSKEKLIGPS 126

Query: 124 ALFFHQGNYHSNLRKLVLNSLSLERLRTLVPCIEAAAISATDSWAAAGHVINTFLEMKKY 183
           ALFFHQG+YHS++RKLV +S   E +R L+P IE  A+S+  SWA    +++T+ EMKK+
Sbjct: 127 ALFFHQGDYHSHIRKLVQSSFYPETIRKLIPDIEHIALSSLQSWANM-PIVSTYQEMKKF 186

Query: 184 SFEVGIIAVFGKLEDEYKEKLKQNYCILDKGYNCFPTRLPGTAYSKALSARKKLREILGE 243
           +F+VGI+A+FG LE  YKE LK NY I+DKGYN FP  LPGT+Y KAL ARK+L+ I+ E
Sbjct: 187 AFDVGILAIFGHLESSYKEILKHNYNIVDKGYNSFPMSLPGTSYHKALMARKQLKTIVSE 246

Query: 244 IIMERREKRVTERDLVGHLLNFRDENGESLSEDQIADNIIGVLFAAQDTTATVLTWILKY 303
           II ERREKR  + D +GHLLNF++E G  L+++QIADNIIGVLFAAQDTTA+ LTWILKY
Sbjct: 247 IICERREKRALQTDFLGHLLNFKNEKGRVLTQEQIADNIIGVLFAAQDTTASCLTWILKY 306

Query: 304 LHDNFKLFEAVKAEQMEIHRRNGEGKMPLSWSQIKDMPFTHRVVLESLRMASIISFTFRE 363
           LHD+ KL EAVKAEQ  I+  N   K PL+W Q ++MP TH+V++ESLRMASIISFTFRE
Sbjct: 307 LHDDQKLLEAVKAEQKAIYEENSREKKPLTWRQTRNMPLTHKVIVESLRMASIISFTFRE 366

Query: 364 AVVDVEYKGYLIPKGWKVMPLFRNIHHNHEYFPDPHIFDPSRFEVAPRANTFMPFGNGVH 423
           AVVDVEYKGYLIPKGWKVMPLFRNIHHN +YF +P +FDPSRFEV P+ NTFMPFG+GVH
Sbjct: 367 AVVDVEYKGYLIPKGWKVMPLFRNIHHNPKYFSNPEVFDPSRFEVNPKPNTFMPFGSGVH 426

Query: 424 SCPGNELAKLEILILLHHLITKFRWEVEGSQSGVEYGPFPMPVQGLPARF 473
           +CPGNELAKL+ILI LHHL++ FRWEV+G + G++Y PFP+P  GLPA F
Sbjct: 427 ACPGNELAKLQILIFLHHLVSNFRWEVKGGEKGIQYSPFPIPQNGLPATF 463

[Close]

BLAST of CSPI07G07650 vs. TAIR 10
Match: AT5G45340.1 (cytochrome P450, family 707, subfamily A, polypeptide 3 )

HSP 1 Score: 538.9 bits (1387), Expect = 4.3e-153
Identity = 263/462 (56.93%), Postives = 341/462 (73.81%), Query Frame = 0


Query: 8   LYITILLLLLFIFFSISFLKRTRNENPKSQNSLKLPPGSMGLPWIGETLQLYSQDPNIFF 67
           L++T+    LF+   + F+   R     S   L LPPG+MG P++GET QLYSQDPN+FF
Sbjct: 6   LFLTLSAAALFLCL-LRFIAGVRR---SSSTKLPLPPGTMGYPYVGETFQLYSQDPNVFF 65

Query: 68  SQKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETMIGPAALFFH 127
           + KQRRYG +FKT++LGCPCVM++S  AA+FVLVT +HLF+PT+P SKE M+G  A+FFH
Sbjct: 66  AAKQRRYGSVFKTHVLGCPCVMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFFH 125

Query: 128 QGNYHSNLRKLVLNSLSLERLRTLVPCIEAAAISATDSWAAAGHVINTFLEMKKYSFEVG 187
           QG+YHS LRKLVL +   + +R +VP IE+ A  + +SW   G  +NT+ EMK Y+F V 
Sbjct: 126 QGDYHSKLRKLVLRAFMPDAIRNMVPHIESIAQESLNSW--DGTQLNTYQEMKTYTFNVA 185

Query: 188 IIAVFGKLEDEYKEKLKQNYCILDKGYNCFPTRLPGTAYSKALSARKKLREILGEIIMER 247
           +I++ GK E  Y+E LK+ Y IL+KGYN  P  LPGT + KA+ ARK+L +IL  I+ +R
Sbjct: 186 LISILGKDEVYYREDLKRCYYILEKGYNSMPINLPGTLFHKAMKARKELAQILANILSKR 245

Query: 248 REKRVTERDLVGHLLNFRDENGESLSEDQIADNIIGVLFAAQDTTATVLTWILKYLHDNF 307
           R+   +  DL+G  +    E+   L+++QIADNIIGV+FAA+DTTA+VLTWILKYL DN 
Sbjct: 246 RQNPSSHTDLLGSFM----EDKAGLTDEQIADNIIGVIFAARDTTASVLTWILKYLADNP 305

Query: 308 KLFEAVKAEQMEIHRRNGEGKMPLSWSQIKDMPFTHRVVLESLRMASIISFTFREAVVDV 367
            + EAV  EQM I +   EG+  L+W   K MP T+RV+ E+LR A+I+SFTFREAV DV
Sbjct: 306 TVLEAVTEEQMAIRKDKKEGE-SLTWEDTKKMPLTYRVIQETLRAATILSFTFREAVEDV 365

Query: 368 EYKGYLIPKGWKVMPLFRNIHHNHEYFPDPHIFDPSRFEVAPRANTFMPFGNGVHSCPGN 427
           EY+GYLIPKGWKV+PLFRNIHHN + F DP  FDPSRFEVAP+ NTFMPFG+G+HSCPGN
Sbjct: 366 EYEGYLIPKGWKVLPLFRNIHHNADIFSDPGKFDPSRFEVAPKPNTFMPFGSGIHSCPGN 425

Query: 428 ELAKLEILILLHHLITKFRWEVEGSQSGVEYGPFPMPVQGLP 470
           ELAKLEI +L+HHL TK+RW + G   G++YGPF +P  GLP
Sbjct: 426 ELAKLEISVLIHHLTTKYRWSIVGPSDGIQYGPFALPQNGLP 456

[Close]

BLAST of CSPI07G07650 vs. TAIR 10
Match: AT4G19230.1 (cytochrome P450, family 707, subfamily A, polypeptide 1 )

HSP 1 Score: 535.4 bits (1378), Expect = 4.7e-152
Identity = 258/462 (55.84%), Postives = 343/462 (74.24%), Query Frame = 0


Query: 8   LYITILLLLLFIFFSISFLKRTRNENPKSQNSLKLPPGSMGLPWIGETLQLYSQDPNIFF 67
           L++T+    LF++F    + + R  + K    L LPPG+MG P++GET QLYSQDPN+FF
Sbjct: 6   LFLTLFAGSLFLYFLRCLISQRRFGSSK----LPLPPGTMGWPYVGETFQLYSQDPNVFF 65

Query: 68  SQKQRRYGEIFKTNILGCPCVMLASAAAARFVLVTNAHLFRPTYPKSKETMIGPAALFFH 127
             KQ+RYG +FKT++LGCPCVM++S  AA+FVLVT +HLF+PT+P SKE M+G  A+FFH
Sbjct: 66  QSKQKRYGSVFKTHVLGCPCVMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFFH 125

Query: 128 QGNYHSNLRKLVLNSLSLERLRTLVPCIEAAAISATDSWAAAGHVINTFLEMKKYSFEVG 187
           QG+YH+ LRKLVL +   E +R +VP IE+ A  +  SW   G +INT+ EMK Y+F V 
Sbjct: 126 QGDYHAKLRKLVLRAFMPESIRNMVPDIESIAQDSLRSW--EGTMINTYQEMKTYTFNVA 185

Query: 188 IIAVFGKLEDEYKEKLKQNYCILDKGYNCFPTRLPGTAYSKALSARKKLREILGEIIMER 247
           ++++FGK E  Y+E LK+ Y IL+KGYN  P  LPGT + K++ ARK+L +IL  I+ ER
Sbjct: 186 LLSIFGKDEVLYREDLKRCYYILEKGYNSMPVNLPGTLFHKSMKARKELSQILARILSER 245

Query: 248 REKRVTERDLVGHLLNFRDENGESLSEDQIADNIIGVLFAAQDTTATVLTWILKYLHDNF 307
           R+   +  DL+G  +  ++E    L+++QIADNIIGV+FAA+DTTA+V++WILKYL +N 
Sbjct: 246 RQNGSSHNDLLGSFMGDKEE----LTDEQIADNIIGVIFAARDTTASVMSWILKYLAENP 305

Query: 308 KLFEAVKAEQMEIHRRNGEGKMPLSWSQIKDMPFTHRVVLESLRMASIISFTFREAVVDV 367
            + EAV  EQM I +   EG+  L+W   K MP T RV+ E+LR+ASI+SFTFREAV DV
Sbjct: 306 NVLEAVTEEQMAIRKDKEEGE-SLTWGDTKKMPLTSRVIQETLRVASILSFTFREAVEDV 365

Query: 368 EYKGYLIPKGWKVMPLFRNIHHNHEYFPDPHIFDPSRFEVAPRANTFMPFGNGVHSCPGN 427
           EY+GYLIPKGWKV+PLFRNIHH+ + F +P  FDPSRFEVAP+ NTFMPFGNG HSCPGN
Sbjct: 366 EYEGYLIPKGWKVLPLFRNIHHSADIFSNPGKFDPSRFEVAPKPNTFMPFGNGTHSCPGN 425

Query: 428 ELAKLEILILLHHLITKFRWEVEGSQSGVEYGPFPMPVQGLP 470
           ELAKLE+ I++HHL TK+ W + G+  G++YGPF +P  GLP
Sbjct: 426 ELAKLEMSIMIHHLTTKYSWSIVGASDGIQYGPFALPQNGLP 456

[Close]

BLAST of CSPI07G07650 vs. TAIR 10
Match: AT2G29090.1 (cytochrome P450, family 707, subfamily A, polypeptide 2 )

HSP 1 Score: 515.8 bits (1327), Expect = 3.9e-146
Identity = 252/436 (57.80%), Postives = 319/436 (73.17%), Query Frame = 0


Query: 37  QNSLKLPPGSMGLPWIGETLQLYSQDPNIFFSQKQRRYGEIFKTNILGCPCVMLASAAAA 96
           +  L+LPPGSMGLP+IGETL+LY+++PN FF+ +Q +YG+IFKT+ILGCPCVM++S  AA
Sbjct: 44  EQRLRLPPGSMGLPYIGETLRLYTENPNSFFATRQNKYGDIFKTHILGCPCVMISSPEAA 103

Query: 97  RFVLVTNAHLFRPTYPKSKETMIGPAALFFHQGNYHSNLRKLVLNSLSLERLRTLVPCIE 156
           R VLV+ AHLF+PTYP SKE MIGP ALFFHQG YHS L++LV +S     LR  V  IE
Sbjct: 104 RMVLVSKAHLFKPTYPPSKERMIGPEALFFHQGPYHSTLKRLVQSSFMPSALRPTVSHIE 163

Query: 157 AAAISATDSWAAAGHVINTFLEMKKYSFEVGIIAVFG-KLEDEYKEKLKQNYCILDKGYN 216
              +    SW +    INT   MK+Y+F+V I++ FG K E    + +K  Y  L++GYN
Sbjct: 164 LLVLQTLSSWTSQ-KSINTLEYMKRYAFDVAIMSAFGDKEEPTTIDVIKLLYQRLERGYN 223

Query: 217 CFPTRLPGTAYSKALSARKKLREILGEIIMERREKRVTERDLVGHLLNFRDENGESLSED 276
             P  LPGT + K++ AR +L E L ++I +RRE    E  L+G LL  +D+    LS+ 
Sbjct: 224 SMPLDLPGTLFHKSMKARIELSEELRKVIEKRRENGREEGGLLGVLLGAKDQKRNGLSDS 283

Query: 277 QIADNIIGVLFAAQDTTATVLTWILKYLHDNFKLFEAVKAEQMEIHRRNGEGKMPLSWSQ 336
           QIADNIIGV+FAA DTTA+VLTW+LKYLHD+  L + V  EQ  I ++  +    +SW  
Sbjct: 284 QIADNIIGVIFAATDTTASVLTWLLKYLHDHPNLLQEVSREQFSIRQKIKKENRRISWED 343

Query: 337 IKDMPFTHRVVLESLRMASIISFTFREAVVDVEYKGYLIPKGWKVMPLFRNIHHNHEYFP 396
            + MP T RV+ E+LR AS++SFTFREAV DVEY GYLIPKGWKV+PLFR IHH+ E+FP
Sbjct: 344 TRKMPLTTRVIQETLRAASVLSFTFREAVQDVEYDGYLIPKGWKVLPLFRRIHHSSEFFP 403

Query: 397 DPHIFDPSRFEVAPRANTFMPFGNGVHSCPGNELAKLEILILLHHLITKFRWEVEGSQSG 456
           DP  FDPSRFEVAP+  T+MPFGNGVHSCPG+ELAKLE+LILLHHL T FRWEV G + G
Sbjct: 404 DPEKFDPSRFEVAPKPYTYMPFGNGVHSCPGSELAKLEMLILLHHLTTSFRWEVIGDEEG 463

Query: 457 VEYGPFPMPVQGLPAR 472
           ++YGPFP+P +GLP R
Sbjct: 464 IQYGPFPVPKKGLPIR 478

[Close]

BLAST of CSPI07G07650 vs. TAIR 10
Match: AT2G29090.2 (cytochrome P450, family 707, subfamily A, polypeptide 2 )

HSP 1 Score: 515.8 bits (1327), Expect = 3.9e-146
Identity = 252/436 (57.80%), Postives = 319/436 (73.17%), Query Frame = 0


Query: 37  QNSLKLPPGSMGLPWIGETLQLYSQDPNIFFSQKQRRYGEIFKTNILGCPCVMLASAAAA 96
           +  L+LPPGSMGLP+IGETL+LY+++PN FF+ +Q +YG+IFKT+ILGCPCVM++S  AA
Sbjct: 44  EQRLRLPPGSMGLPYIGETLRLYTENPNSFFATRQNKYGDIFKTHILGCPCVMISSPEAA 103

Query: 97  RFVLVTNAHLFRPTYPKSKETMIGPAALFFHQGNYHSNLRKLVLNSLSLERLRTLVPCIE 156
           R VLV+ AHLF+PTYP SKE MIGP ALFFHQG YHS L++LV +S     LR  V  IE
Sbjct: 104 RMVLVSKAHLFKPTYPPSKERMIGPEALFFHQGPYHSTLKRLVQSSFMPSALRPTVSHIE 163

Query: 157 AAAISATDSWAAAGHVINTFLEMKKYSFEVGIIAVFG-KLEDEYKEKLKQNYCILDKGYN 216
              +    SW +    INT   MK+Y+F+V I++ FG K E    + +K  Y  L++GYN
Sbjct: 164 LLVLQTLSSWTSQ-KSINTLEYMKRYAFDVAIMSAFGDKEEPTTIDVIKLLYQRLERGYN 223

Query: 217 CFPTRLPGTAYSKALSARKKLREILGEIIMERREKRVTERDLVGHLLNFRDENGESLSED 276
             P  LPGT + K++ AR +L E L ++I +RRE    E  L+G LL  +D+    LS+ 
Sbjct: 224 SMPLDLPGTLFHKSMKARIELSEELRKVIEKRRENGREEGGLLGVLLGAKDQKRNGLSDS 283

Query: 277 QIADNIIGVLFAAQDTTATVLTWILKYLHDNFKLFEAVKAEQMEIHRRNGEGKMPLSWSQ 336
           QIADNIIGV+FAA DTTA+VLTW+LKYLHD+  L + V  EQ  I ++  +    +SW  
Sbjct: 284 QIADNIIGVIFAATDTTASVLTWLLKYLHDHPNLLQEVSREQFSIRQKIKKENRRISWED 343

Query: 337 IKDMPFTHRVVLESLRMASIISFTFREAVVDVEYKGYLIPKGWKVMPLFRNIHHNHEYFP 396
            + MP T RV+ E+LR AS++SFTFREAV DVEY GYLIPKGWKV+PLFR IHH+ E+FP
Sbjct: 344 TRKMPLTTRVIQETLRAASVLSFTFREAVQDVEYDGYLIPKGWKVLPLFRRIHHSSEFFP 403

Query: 397 DPHIFDPSRFEVAPRANTFMPFGNGVHSCPGNELAKLEILILLHHLITKFRWEVEGSQSG 456
           DP  FDPSRFEVAP+  T+MPFGNGVHSCPG+ELAKLE+LILLHHL T FRWEV G + G
Sbjct: 404 DPEKFDPSRFEVAPKPYTYMPFGNGVHSCPGSELAKLEMLILLHHLTTSFRWEVIGDEEG 463

Query: 457 VEYGPFPMPVQGLPAR 472
           ++YGPFP+P +GLP R
Sbjct: 464 IQYGPFPVPKKGLPIR 478

The following BLAST results are available for this feature:

BLAST of CSPI07G07650 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LJK2	3.0e-180	65.53	Abscisic acid 8'-hydroxylase 4 OS=Arabidopsis thaliana OX=3702 GN=CYP707A4 PE=2 ...	[more]
A9QNE7	2.6e-179	66.31	Abscisic acid 8'-hydroxylase CYP707A1 OS=Solanum lycopersicum OX=4081 GN=CYP707A...	[more]
Q09J78	9.2e-169	63.45	Abscisic acid 8'-hydroxylase 2 OS=Oryza sativa subsp. indica OX=39946 GN=CYP707A...	[more]
Q0J185	1.6e-168	60.04	Abscisic acid 8'-hydroxylase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP70...	[more]
A2Z212	3.5e-168	60.04	Abscisic acid 8'-hydroxylase 3 OS=Oryza sativa subsp. indica OX=39946 GN=CYP707A...	[more]

BLAST of CSPI07G07650 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3BZQ3	1.5e-270	96.46	abscisic acid 8'-hydroxylase 4 OS=Cucumis melo OX=3656 GN=LOC103495236 PE=3 SV=1	[more]
A0A0A0K6K8	1.1e-257	99.54	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G073680 PE=3 SV=1	[more]
A0A5A7SMJ8	1.2e-248	97.24	Abscisic acid 8'-hydroxylase 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s...	[more]
A0A6J1I8M8	1.3e-242	87.53	abscisic acid 8'-hydroxylase 4-like OS=Cucurbita maxima OX=3661 GN=LOC111471004 ...	[more]
A0A6J1EHU3	3.8e-242	86.88	abscisic acid 8'-hydroxylase 4-like OS=Cucurbita moschata OX=3662 GN=LOC11143448...	[more]

BLAST of CSPI07G07650 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004137112.1	3.5e-282	99.59	abscisic acid 8'-hydroxylase 4 [Cucumis sativus] >KAE8645865.1 hypothetical prot...	[more]
XP_008454947.1	3.1e-270	96.46	PREDICTED: abscisic acid 8'-hydroxylase 4 [Cucumis melo]	[more]
XP_038887333.1	6.8e-254	91.32	abscisic acid 8'-hydroxylase 4 [Benincasa hispida]	[more]
KAA0031296.1	2.5e-248	97.24	abscisic acid 8'-hydroxylase 4 [Cucumis melo var. makuwa] >TYK06748.1 abscisic a...	[more]
XP_023553809.1	2.4e-243	87.53	abscisic acid 8'-hydroxylase 4-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of CSPI07G07650 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G19270.1	2.2e-181	65.53	cytochrome P450, family 707, subfamily A, polypeptide 4	[more]
AT5G45340.1	4.3e-153	56.93	cytochrome P450, family 707, subfamily A, polypeptide 3	[more]
AT4G19230.1	4.7e-152	55.84	cytochrome P450, family 707, subfamily A, polypeptide 1	[more]
AT2G29090.1	3.9e-146	57.80	cytochrome P450, family 707, subfamily A, polypeptide 2	[more]
AT2G29090.2	3.9e-146	57.80	cytochrome P450, family 707, subfamily A, polypeptide 2	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 243..252 score: 47.79 coord: 252..263 score: 39.37 coord: 115..132 score: 36.48 coord: 172..183 score: 33.62
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 27..281 e-value: 7.4E-58 score: 196.4
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 104..121 score: 21.19 coord: 171..189 score: 22.97 coord: 211..235 score: 27.21 coord: 242..252 score: 47.18 coord: 252..275 score: 30.32
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 2..302 e-value: 6.2E-79 score: 267.8
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 6..301
None	No IPR available	PANTHER	PTHR24286:SF103	CYTOCHROME P450 FAMILY ABA 8'-HYDROXYLASE	coord: 9..302
None	No IPR available	PANTHER	PTHR24286	CYTOCHROME P450 26	coord: 9..302
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 245..254

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI07G07650.1	CSPI07G07650.1	mRNA
CSPI07G07650.2	CSPI07G07650.2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016125	sterol metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0010295	(+)-abscisic acid 8'-hydroxylase activity
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen