CSPI07G07500 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI07G07500
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	CBS domain-containing protein CBSX1, chloroplastic-like
Location	Chr7: 5364006 .. 5365691 (-)
RNA-Seq Expression	CSPI07G07500
Synteny	CSPI07G07500

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GTTTTCTATTCTTTACGATTCTCCGCCGCAATAACCCATTAAAGAAATGGCCTCAATTTCTACTCCCTACGTTCCCTCTGTTTTTCCCAACTCACGATTGCCGACGACCCAACTCCGGCATGCCGGGTATCGGTCTCCAGTTGTCGCGTTGGCATTTTCCGGTCACCGTGTGTCCAGTTCTATTCCGGTAGGCTTGAATGGTTAATTTTTCTTGATTTTATAAGACACTGTGAATTTATTTGGGTTTATATCAGTATCAATATCAATTATATGCGTGCTTGTGTTTTGTTTTTTCAGTTTAGGAATGGATCGTATGCGGTTGGAGATTTCATGACGAAGAAGGGGAATTTGCAGGTTCTTAAGCCTTCCACGAGTGTTGAGGAAGGTATTTTAAATTTATAATTTAATCGTCTCGTCAAACTGCCATTTTAGTCGTTTTGTGTCATATATATGTTTTGAAATTTCATTTTGATTTTGTACTTGAAGTTTACAAATTAAGCTTTCAAATTTATACGATCTAAAACCTTCTAACTTTGAGGTTTGATCGGATGAAACAGCCTTGGAAGTTCTGGTGGAGAAGAGTCTATCTGGGTTTCCTGTAGTTGATGACGATTGGAAACTGGTAATGATCGATAATAATAACAACAGCATTCATTTTGATAAAGAATGTATTTTCTTTGAGGAAATGACTGGAAAATAATCTTCAAATTCCATAACAGGTTGGCGTTGTCTCAGATTATGATTTGTTAGCATTGGACTCAATTTCAGGTACGTGCCCATATTACATATATATAGACATACTCAAGATTAATCACCAACATCTTGAACTTATAATCAACTTTTATTTGAAATGCTGATATATATATATATATACAGGTGTGGGTGGTGGTGACATCATTAATATATTTCCAGATGTTAACTGTAGCTGGGAAGTAAGTAACAAAAAGATTAAAATATGTTCTTTTTAATTTTCCATTTTACTAATTTATTTAACACTGTTCAAGATAACTAAGTTGAATTAATTATGTTACCGTGGCAGAGCTTCAAGTTGATACAGAAATTGTTGAGCAAAAAGAATGGAGAAGTTGTAGGAGATTTGATGACACCTGCTCCATTGGTTGTTAGTGAAACCATGAACTTTGAAAATGCTGCCAGGTCTGTGTTCTTAAAAATTAACGTTGATTCCCATGGTTAAATATGAAACTACCAAATTAAATGATAATATGTGACACTAAAAAAGATAATAAGCTAAGAAATTTGATTTTGATTTAATTATTAACATACACAATTAGTGTGTTTCTTAACCTCACAATCCTAATCTTTTCTTATTCCTTTGTGATAGACTATTGCTTGAAACAAAGTTCCATCGTTTACCCGTGGTAGATTGTGAAGGAAAGTTGGTAAGTAAATTAGAGCTTGTATAGTTATGATGCTTCTCACATTATACATATGCAAATCTTTTCTTCACATTATAACTAATGGACATCTCTTTCTTGATCAATTGAAGGTGGGAATCATTACAAGAGAAGACATAGTCAGAGTTGGCTTAGAGATGAAACGCACACAAGAAGATACATTTTGAAGTTCAAAGAATCAAACAAATAATGTTATCACCCATTATTTTTTATAGACACATAACAATTTTGACTCTCATTTAATGTATAGCACCATTCATTTATCCCATAG

mRNA sequence

GTTTTCTATTCTTTACGATTCTCCGCCGCAATAACCCATTAAAGAAATGGCCTCAATTTCTACTCCCTACGTTCCCTCTGTTTTTCCCAACTCACGATTGCCGACGACCCAACTCCGGCATGCCGGGTATCGGTCTCCAGTTGTCGCGTTGGCATTTTCCGGTCACCGTGTGTCCAGTTCTATTCCGTTTAGGAATGGATCGTATGCGGTTGGAGATTTCATGACGAAGAAGGGGAATTTGCAGGTTCTTAAGCCTTCCACGAGTGTTGAGGAAGCCTTGGAAGTTCTGGTGGAGAAGAGTCTATCTGGGTTTCCTGTAGTTGATGACGATTGGAAACTGGTTGGCGTTGTCTCAGATTATGATTTGTTAGCATTGGACTCAATTTCAGGTGTGGGTGGTGGTGACATCATTAATATATTTCCAGATGTTAACTGTAGCTGGGAAAGCTTCAAGTTGATACAGAAATTGTTGAGCAAAAAGAATGGAGAAGTTGTAGGAGATTTGATGACACCTGCTCCATTGGTTGTTAGTGAAACCATGAACTTTGAAAATGCTGCCAGACTATTGCTTGAAACAAAGTTCCATCGTTTACCCGTGGTAGATTGTGAAGGAAAGTTGGTGGGAATCATTACAAGAGAAGACATAGTCAGAGTTGGCTTAGAGATGAAACGCACACAAGAAGATACATTTTGAAGTTCAAAGAATCAAACAAATAATGTTATCACCCATTATTTTTTATAGACACATAACAATTTTGACTCTCATTTAATGTATAGCACCATTCATTTATCCCATAG

Coding sequence (CDS)

ATGGCCTCAATTTCTACTCCCTACGTTCCCTCTGTTTTTCCCAACTCACGATTGCCGACGACCCAACTCCGGCATGCCGGGTATCGGTCTCCAGTTGTCGCGTTGGCATTTTCCGGTCACCGTGTGTCCAGTTCTATTCCGTTTAGGAATGGATCGTATGCGGTTGGAGATTTCATGACGAAGAAGGGGAATTTGCAGGTTCTTAAGCCTTCCACGAGTGTTGAGGAAGCCTTGGAAGTTCTGGTGGAGAAGAGTCTATCTGGGTTTCCTGTAGTTGATGACGATTGGAAACTGGTTGGCGTTGTCTCAGATTATGATTTGTTAGCATTGGACTCAATTTCAGGTGTGGGTGGTGGTGACATCATTAATATATTTCCAGATGTTAACTGTAGCTGGGAAAGCTTCAAGTTGATACAGAAATTGTTGAGCAAAAAGAATGGAGAAGTTGTAGGAGATTTGATGACACCTGCTCCATTGGTTGTTAGTGAAACCATGAACTTTGAAAATGCTGCCAGACTATTGCTTGAAACAAAGTTCCATCGTTTACCCGTGGTAGATTGTGAAGGAAAGTTGGTGGGAATCATTACAAGAGAAGACATAGTCAGAGTTGGCTTAGAGATGAAACGCACACAAGAAGATACATTTTGA

Protein sequence

MASISTPYVPSVFPNSRLPTTQLRHAGYRSPVVALAFSGHRVSSSIPFRNGSYAVGDFMTKKGNLQVLKPSTSVEEALEVLVEKSLSGFPVVDDDWKLVGVVSDYDLLALDSISGVGGGDIINIFPDVNCSWESFKLIQKLLSKKNGEVVGDLMTPAPLVVSETMNFENAARLLLETKFHRLPVVDCEGKLVGIITREDIVRVGLEMKRTQEDTF*

Homology

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BLAST of CSPI07G07500 vs. ExPASy Swiss-Prot
Match: Q9C5D0 (CBS domain-containing protein CBSX2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CBSX2 PE=1 SV=1)

HSP 1 Score: 228.0 bits (580), Expect = 1.0e-58
Identity = 117/204 (57.35%), Postives = 154/204 (75.49%), Query Frame = 0


Query: 16  SRLPTTQLRHAGYRSPVVAL-AFSGHRVS----SSIPFRNGSYAVGDFMTKKGNLQVLKP 75
           S LP +  R +   SP + + AF     S    +S+P +NG Y VGDFMT + NL V+KP
Sbjct: 35  SLLPLSNRRRSSTFSPSITVSAFFAAPASVNNNNSVPAKNGGYTVGDFMTPRQNLHVVKP 94

Query: 76  STSVEEALEVLVEKSLSGFPVVDDDWKLVGVVSDYDLLALDSISGVGGGDIINIFPDVNC 135
           STSV++ALE+LVEK ++G PV+DD+W LVGVVSDYDLLALDSISG    D  N+FPDV+ 
Sbjct: 95  STSVDDALELLVEKKVTGLPVIDDNWTLVGVVSDYDLLALDSISGRSQND-TNLFPDVDS 154

Query: 136 SWESFKLIQKLLSKKNGEVVGDLMTPAPLVVSETMNFENAARLLLETKFHRLPVVDCEGK 195
           +W++F  +QKL+SK  G+VVGDLMTP+PLVV ++ N E+AARLLLETKF RLPVVD +GK
Sbjct: 155 TWKTFNELQKLISKTYGKVVGDLMTPSPLVVRDSTNLEDAARLLLETKFRRLPVVDADGK 214

Query: 196 LVGIITREDIVRVGLEMKRTQEDT 215
           L+GI+TR ++VR  L++KR  E++
Sbjct: 215 LIGILTRGNVVRAALQIKRETENS 237

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BLAST of CSPI07G07500 vs. ExPASy Swiss-Prot
Match: O23193 (CBS domain-containing protein CBSX1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CBSX1 PE=1 SV=2)

HSP 1 Score: 219.5 bits (558), Expect = 3.6e-56
Identity = 115/214 (53.74%), Postives = 160/214 (74.77%), Query Frame = 0


Query: 2   ASISTPYV-----PSVFPNSRLPTTQLRHAGYRSPVVALAFSGHRVSSSIPFRNGSYAVG 61
           +S S+PY+      SV P  +   ++   +  R P  + A     +++S   R+G Y VG
Sbjct: 19  SSPSSPYLLLPRFLSVQPCHKFTFSRSFPSKSRIPSASSAAGSTLMTNSSSPRSGVYTVG 78

Query: 62  DFMTKKGNLQVLKPSTSVEEALEVLVEKSLSGFPVVDDDWKLVGVVSDYDLLALDSISGV 121
           +FMTKK +L V+KP+T+V+EALE+LVE  ++GFPV+D+DWKLVG+VSDYDLLALDSISG 
Sbjct: 79  EFMTKKEDLHVVKPTTTVDEALELLVENRITGFPVIDEDWKLVGLVSDYDLLALDSISGS 138

Query: 122 GGGDIINIFPDVNCSWESFKLIQKLLSKKNGEVVGDLMTPAPLVVSETMNFENAARLLLE 181
           G  +  ++FP+V+ +W++F  +QKLLSK NG++VGDLMTPAPLVV E  N E+AA++LLE
Sbjct: 139 GRTE-NSMFPEVDSTWKTFNAVQKLLSKTNGKLVGDLMTPAPLVVEEKTNLEDAAKILLE 198

Query: 182 TKFHRLPVVDCEGKLVGIITREDIVRVGLEMKRT 211
           TK+ RLPVVD +GKLVGIITR ++VR  L++KR+
Sbjct: 199 TKYRRLPVVDSDGKLVGIITRGNVVRAALQIKRS 231

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BLAST of CSPI07G07500 vs. ExPASy Swiss-Prot
Match: O67820 (Inosine-5'-monophosphate dehydrogenase OS=Aquifex aeolicus (strain VF5) OX=224324 GN=guaB PE=3 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 1.5e-09
Identity = 42/139 (30.22%), Postives = 74/139 (53.24%), Query Frame = 0


Query: 64  NLQVLKPSTSVEEALEVLVEKSLSGFPVVDDDWKLVGVVSDYDLLALDSISGVGGGDIIN 123
           N   +KP T V+EAL+++ +  +SG PVVD++ KL+G++++ DL                
Sbjct: 99  NPVTVKPDTRVKEALDIMAKYKISGVPVVDEERKLIGILTNRDLRF-------------- 158

Query: 124 IFPDVNCSWESFKLIQKLLSKKNGEVVGDLMTPAPLVVSETMNFENAARLLLETKFHRLP 183
           I P+     +  K + + ++K+N       +  AP    E +  + A  +  + K  +LP
Sbjct: 159 IKPE-----DYSKPVSEFMTKEN-------LITAP----EGITLDEAEEIFRKYKIEKLP 207

Query: 184 VVDCEGKLVGIITREDIVR 203
           +VD EGK+ G+IT +DIV+
Sbjct: 219 IVDKEGKIKGLITIKDIVK 207

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BLAST of CSPI07G07500 vs. ExPASy Swiss-Prot
Match: Q9UY49 (Inosine-5'-monophosphate dehydrogenase OS=Pyrococcus abyssi (strain GE5 / Orsay) OX=272844 GN=guaB PE=3 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 3.3e-09
Identity = 38/134 (28.36%), Postives = 67/134 (50.00%), Query Frame = 0


Query: 68  LKPSTSVEEALEVLVEKSLSGFPVVDDDWKLVGVVSDYDLLALDSISGVGGGDIINIFPD 127
           + P  +V+ AL ++ +  + G PVV+++ K+VG++S  D+ A                  
Sbjct: 106 ISPEETVDFALFLMEKHDIDGLPVVENE-KVVGIISKKDIAA------------------ 165

Query: 128 VNCSWESFKLIQKLLSKKNGEVVGDLMTPAPLVVSETMNFENAARLLLETKFHRLPVVDC 187
                            + G++V +LMT   + V E +  E A ++++E +  RLPVVD 
Sbjct: 166 -----------------REGKLVKELMTKDVITVPENIEVEEALKIMIENRIDRLPVVDK 203

Query: 188 EGKLVGIITREDIV 202
           EG+L+G+IT  D+V
Sbjct: 226 EGRLIGLITMSDLV 203

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BLAST of CSPI07G07500 vs. ExPASy Swiss-Prot
Match: Q58821 (Uncharacterized protein MJ1426 OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) OX=243232 GN=MJ1426 PE=4 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 7.3e-09
Identity = 41/133 (30.83%), Postives = 69/133 (51.88%), Query Frame = 0


Query: 76  EALEVLVEKSLSGFPVVDDDWKLVGVVSDYDLLALDSISGVGGGDIINIFPDVNCSWE-- 135
           + + +  +  +SG PV++ D KLVG++S+ D++           D+  I P      E  
Sbjct: 35  DVIRLFRKNKISGAPVLNKDGKLVGIISESDIV---KTIVTHNEDLNLILPSPLDLIELP 94

Query: 136 ---SFKLIQKLLSKKNG--EVVGDLMTPAPLVVSETMNFENAARLLLETKFHRLPVVDCE 195
              + K+ + +   KN     V D+MT   +V    M   +AA+L+++    RLPVVD E
Sbjct: 95  LKTALKIEEFMEDLKNALKTKVRDVMTRKVIVAKPDMTINDAAKLMVKNNIKRLPVVDDE 154

Query: 196 GKLVGIITREDIV 202
           G L+GI+TR D++
Sbjct: 155 GNLIGIVTRGDLI 164

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BLAST of CSPI07G07500 vs. ExPASy TrEMBL
Match: A0A0A0K7X9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G073520 PE=4 SV=1)

HSP 1 Score: 427.6 bits (1098), Expect = 3.2e-116
Identity = 215/215 (100.00%), Postives = 215/215 (100.00%), Query Frame = 0


Query: 1   MASISTPYVPSVFPNSRLPTTQLRHAGYRSPVVALAFSGHRVSSSIPFRNGSYAVGDFMT 60
           MASISTPYVPSVFPNSRLPTTQLRHAGYRSPVVALAFSGHRVSSSIPFRNGSYAVGDFMT
Sbjct: 1   MASISTPYVPSVFPNSRLPTTQLRHAGYRSPVVALAFSGHRVSSSIPFRNGSYAVGDFMT 60

Query: 61  KKGNLQVLKPSTSVEEALEVLVEKSLSGFPVVDDDWKLVGVVSDYDLLALDSISGVGGGD 120
           KKGNLQVLKPSTSVEEALEVLVEKSLSGFPVVDDDWKLVGVVSDYDLLALDSISGVGGGD
Sbjct: 61  KKGNLQVLKPSTSVEEALEVLVEKSLSGFPVVDDDWKLVGVVSDYDLLALDSISGVGGGD 120

Query: 121 IINIFPDVNCSWESFKLIQKLLSKKNGEVVGDLMTPAPLVVSETMNFENAARLLLETKFH 180
           IINIFPDVNCSWESFKLIQKLLSKKNGEVVGDLMTPAPLVVSETMNFENAARLLLETKFH
Sbjct: 121 IINIFPDVNCSWESFKLIQKLLSKKNGEVVGDLMTPAPLVVSETMNFENAARLLLETKFH 180

Query: 181 RLPVVDCEGKLVGIITREDIVRVGLEMKRTQEDTF 216
           RLPVVDCEGKLVGIITREDIVRVGLEMKRTQEDTF
Sbjct: 181 RLPVVDCEGKLVGIITREDIVRVGLEMKRTQEDTF 215

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BLAST of CSPI07G07500 vs. ExPASy TrEMBL
Match: A0A5D3C646 (CBS domain-containing protein CBSX2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G00630 PE=4 SV=1)

HSP 1 Score: 387.9 bits (995), Expect = 2.8e-104
Identity = 199/220 (90.45%), Postives = 205/220 (93.18%), Query Frame = 0


Query: 1   MASISTPYVPSVFPNSRLPTTQLRHAG---------YRSPVVALAFSGHRVSSSIPFR-N 60
           MASISTPYVPS F NSRLPTTQ RHAG         +RSPV+ALAFSGHRV+SSIPFR N
Sbjct: 1   MASISTPYVPSFFSNSRLPTTQFRHAGTFDSSPSSLFRSPVLALAFSGHRVASSIPFRNN 60

Query: 61  GSYAVGDFMTKKGNLQVLKPSTSVEEALEVLVEKSLSGFPVVDDDWKLVGVVSDYDLLAL 120
           GSY VGDFMTKKGNL VLKPSTS+EEALEVLVEKS+SGFPVVDDDWKLVGVVSDYDLLAL
Sbjct: 61  GSYTVGDFMTKKGNLLVLKPSTSIEEALEVLVEKSVSGFPVVDDDWKLVGVVSDYDLLAL 120

Query: 121 DSISGVGGGDIINIFPDVNCSWESFKLIQKLLSKKNGEVVGDLMTPAPLVVSETMNFENA 180
           DSISGVGGGDIINIFPDVNCSWESFKLIQKLLSKKNGE+VGDLMTPAPLVVSETMNFENA
Sbjct: 121 DSISGVGGGDIINIFPDVNCSWESFKLIQKLLSKKNGEIVGDLMTPAPLVVSETMNFENA 180

Query: 181 ARLLLETKFHRLPVVDCEGKLVGIITREDIVRVGLEMKRT 211
           ARLLLETKFHRLPVVDCEGKLVGIITREDIVRVGLEMKRT
Sbjct: 181 ARLLLETKFHRLPVVDCEGKLVGIITREDIVRVGLEMKRT 220

[Close]

BLAST of CSPI07G07500 vs. ExPASy TrEMBL
Match: A0A1S3C189 (CBS domain-containing protein CBSX2, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103495670 PE=4 SV=1)

HSP 1 Score: 367.9 bits (943), Expect = 3.0e-98
Identity = 188/208 (90.38%), Postives = 194/208 (93.27%), Query Frame = 0


Query: 13  FPNSRLPTTQLRHAG---------YRSPVVALAFSGHRVSSSIPFR-NGSYAVGDFMTKK 72
           F NSRLPTTQ RHAG         +RSPV+ALAFSGHRV+SSIPFR NGSY VGDFMTKK
Sbjct: 21  FSNSRLPTTQFRHAGTFDSSPSSLFRSPVLALAFSGHRVASSIPFRNNGSYTVGDFMTKK 80

Query: 73  GNLQVLKPSTSVEEALEVLVEKSLSGFPVVDDDWKLVGVVSDYDLLALDSISGVGGGDII 132
           GNL VLKPSTS+EEALEVLVEKS+SGFPVVDDDWKLVGVVSDYDLLALDSISGVGGGDII
Sbjct: 81  GNLLVLKPSTSIEEALEVLVEKSVSGFPVVDDDWKLVGVVSDYDLLALDSISGVGGGDII 140

Query: 133 NIFPDVNCSWESFKLIQKLLSKKNGEVVGDLMTPAPLVVSETMNFENAARLLLETKFHRL 192
           NIFPDVNCSWESFKLIQKLLSKKNGE+VGDLMTPAPLVVSETMNFENAARLLLETKFHRL
Sbjct: 141 NIFPDVNCSWESFKLIQKLLSKKNGEIVGDLMTPAPLVVSETMNFENAARLLLETKFHRL 200

Query: 193 PVVDCEGKLVGIITREDIVRVGLEMKRT 211
           PVVDCEGKLVGIITREDIVRVGLEMKRT
Sbjct: 201 PVVDCEGKLVGIITREDIVRVGLEMKRT 228

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BLAST of CSPI07G07500 vs. ExPASy TrEMBL
Match: A0A5A7SKP3 (CBS domain-containing protein CBSX2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold139G00630 PE=4 SV=1)

HSP 1 Score: 340.9 bits (873), Expect = 3.9e-90
Identity = 184/220 (83.64%), Postives = 192/220 (87.27%), Query Frame = 0


Query: 1   MASISTPYVPSVFPNSRLPTTQLRHAG---------YRSPVVALAFSGHRVSSSIPFR-N 60
           MASISTPYVPS F NSRLPTTQ RHAG         +RSPV+ALAFSGHRV+SSIPFR N
Sbjct: 1   MASISTPYVPSFFSNSRLPTTQFRHAGTFDSSPSSLFRSPVLALAFSGHRVASSIPFRNN 60

Query: 61  GSYAVGDFMTKKGNLQVLKPSTSVEEALEVLVEKSLSGFPVVDDDWKLVGVVSDYDLLAL 120
           GSY VGDFMTKKGNL VLKPSTS+EEALEVLVEKS+SGFPVVDDDWKLVGVVSDYDLLAL
Sbjct: 61  GSYTVGDFMTKKGNLLVLKPSTSIEEALEVLVEKSVSGFPVVDDDWKLVGVVSDYDLLAL 120

Query: 121 DSISGVGGGDIINIFPDVNCSWESFKLIQKLLSKKNGEVVGDLMTPAPLVVSETMNFENA 180
           DSIS    G II +       W+SFKLIQKLLSKKNGE+VGDLMTPAPLVVSETMNFENA
Sbjct: 121 DSIS---VGLIIMLL------WQSFKLIQKLLSKKNGEIVGDLMTPAPLVVSETMNFENA 180

Query: 181 ARLLLETKFHRLPVVDCEGKLVGIITREDIVRVGLEMKRT 211
           ARLLLETKFHRLPVVDCEGKLVGIITREDIVRVGLEMKRT
Sbjct: 181 ARLLLETKFHRLPVVDCEGKLVGIITREDIVRVGLEMKRT 211

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BLAST of CSPI07G07500 vs. ExPASy TrEMBL
Match: A0A6J1DN09 (CBS domain-containing protein CBSX1, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111022731 PE=4 SV=1)

HSP 1 Score: 303.5 bits (776), Expect = 6.9e-79
Identity = 161/220 (73.18%), Postives = 181/220 (82.27%), Query Frame = 0


Query: 1   MASISTPYV-PSVFPNSRLPTTQLRH------AGYRSPVVALAFSGHRVSSSIPFRNGSY 60
           MASISTPYV P++ PNSR    + R       +  RSP V  A SGHR+++S P R+GSY
Sbjct: 1   MASISTPYVPPALLPNSRPLQRRFRSSAAFSPSSNRSPSVVSALSGHRIANSAPSRSGSY 60

Query: 61  AVGDFMTKKGNLQVLKPSTSVEEALEVLVEKSLSGFPVVDDDWKLVGVVSDYDLLALDSI 120
            VGDFMTKKGNLQV+KPST+++EALEVLVE  LSGFPVVD DWKLVGVVSDYDLLA+DSI
Sbjct: 61  TVGDFMTKKGNLQVVKPSTTIDEALEVLVENKLSGFPVVDGDWKLVGVVSDYDLLAVDSI 120

Query: 121 SGVGGGD-IINIFPDVNCSWESFKLIQKLLSKKNGEVVGDLMTPAPLVVSETMNFENAAR 180
           SGVGGGD   NIFPDVN SW+SFK IQKL+SK NGEV+GDLMTPAPLVV ET + ENAAR
Sbjct: 121 SGVGGGDWETNIFPDVNISWKSFKEIQKLVSKTNGEVIGDLMTPAPLVVRETTDLENAAR 180

Query: 181 LLLETKFHRLPVVDCEGKLVGIITREDIVRVGLE-MKRTQ 212
           LLLETKFH LPVVDC+GKLVG ITREDIV+ GL+ MKRTQ
Sbjct: 181 LLLETKFHSLPVVDCDGKLVGNITREDIVKAGLQIMKRTQ 220

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BLAST of CSPI07G07500 vs. NCBI nr
Match: XP_004136971.1 (CBS domain-containing protein CBSX2, chloroplastic [Cucumis sativus] >KGN43916.1 hypothetical protein Csa_017254 [Cucumis sativus])

HSP 1 Score: 427.6 bits (1098), Expect = 6.6e-116
Identity = 215/215 (100.00%), Postives = 215/215 (100.00%), Query Frame = 0


Query: 1   MASISTPYVPSVFPNSRLPTTQLRHAGYRSPVVALAFSGHRVSSSIPFRNGSYAVGDFMT 60
           MASISTPYVPSVFPNSRLPTTQLRHAGYRSPVVALAFSGHRVSSSIPFRNGSYAVGDFMT
Sbjct: 1   MASISTPYVPSVFPNSRLPTTQLRHAGYRSPVVALAFSGHRVSSSIPFRNGSYAVGDFMT 60

Query: 61  KKGNLQVLKPSTSVEEALEVLVEKSLSGFPVVDDDWKLVGVVSDYDLLALDSISGVGGGD 120
           KKGNLQVLKPSTSVEEALEVLVEKSLSGFPVVDDDWKLVGVVSDYDLLALDSISGVGGGD
Sbjct: 61  KKGNLQVLKPSTSVEEALEVLVEKSLSGFPVVDDDWKLVGVVSDYDLLALDSISGVGGGD 120

Query: 121 IINIFPDVNCSWESFKLIQKLLSKKNGEVVGDLMTPAPLVVSETMNFENAARLLLETKFH 180
           IINIFPDVNCSWESFKLIQKLLSKKNGEVVGDLMTPAPLVVSETMNFENAARLLLETKFH
Sbjct: 121 IINIFPDVNCSWESFKLIQKLLSKKNGEVVGDLMTPAPLVVSETMNFENAARLLLETKFH 180

Query: 181 RLPVVDCEGKLVGIITREDIVRVGLEMKRTQEDTF 216
           RLPVVDCEGKLVGIITREDIVRVGLEMKRTQEDTF
Sbjct: 181 RLPVVDCEGKLVGIITREDIVRVGLEMKRTQEDTF 215

[Close]

BLAST of CSPI07G07500 vs. NCBI nr
Match: TYK06762.1 (CBS domain-containing protein CBSX2 [Cucumis melo var. makuwa])

HSP 1 Score: 387.9 bits (995), Expect = 5.8e-104
Identity = 199/220 (90.45%), Postives = 205/220 (93.18%), Query Frame = 0


Query: 1   MASISTPYVPSVFPNSRLPTTQLRHAG---------YRSPVVALAFSGHRVSSSIPFR-N 60
           MASISTPYVPS F NSRLPTTQ RHAG         +RSPV+ALAFSGHRV+SSIPFR N
Sbjct: 1   MASISTPYVPSFFSNSRLPTTQFRHAGTFDSSPSSLFRSPVLALAFSGHRVASSIPFRNN 60

Query: 61  GSYAVGDFMTKKGNLQVLKPSTSVEEALEVLVEKSLSGFPVVDDDWKLVGVVSDYDLLAL 120
           GSY VGDFMTKKGNL VLKPSTS+EEALEVLVEKS+SGFPVVDDDWKLVGVVSDYDLLAL
Sbjct: 61  GSYTVGDFMTKKGNLLVLKPSTSIEEALEVLVEKSVSGFPVVDDDWKLVGVVSDYDLLAL 120

Query: 121 DSISGVGGGDIINIFPDVNCSWESFKLIQKLLSKKNGEVVGDLMTPAPLVVSETMNFENA 180
           DSISGVGGGDIINIFPDVNCSWESFKLIQKLLSKKNGE+VGDLMTPAPLVVSETMNFENA
Sbjct: 121 DSISGVGGGDIINIFPDVNCSWESFKLIQKLLSKKNGEIVGDLMTPAPLVVSETMNFENA 180

Query: 181 ARLLLETKFHRLPVVDCEGKLVGIITREDIVRVGLEMKRT 211
           ARLLLETKFHRLPVVDCEGKLVGIITREDIVRVGLEMKRT
Sbjct: 181 ARLLLETKFHRLPVVDCEGKLVGIITREDIVRVGLEMKRT 220

[Close]

BLAST of CSPI07G07500 vs. NCBI nr
Match: XP_008455519.1 (PREDICTED: CBS domain-containing protein CBSX2, chloroplastic [Cucumis melo])

HSP 1 Score: 367.9 bits (943), Expect = 6.2e-98
Identity = 188/208 (90.38%), Postives = 194/208 (93.27%), Query Frame = 0


Query: 13  FPNSRLPTTQLRHAG---------YRSPVVALAFSGHRVSSSIPFR-NGSYAVGDFMTKK 72
           F NSRLPTTQ RHAG         +RSPV+ALAFSGHRV+SSIPFR NGSY VGDFMTKK
Sbjct: 21  FSNSRLPTTQFRHAGTFDSSPSSLFRSPVLALAFSGHRVASSIPFRNNGSYTVGDFMTKK 80

Query: 73  GNLQVLKPSTSVEEALEVLVEKSLSGFPVVDDDWKLVGVVSDYDLLALDSISGVGGGDII 132
           GNL VLKPSTS+EEALEVLVEKS+SGFPVVDDDWKLVGVVSDYDLLALDSISGVGGGDII
Sbjct: 81  GNLLVLKPSTSIEEALEVLVEKSVSGFPVVDDDWKLVGVVSDYDLLALDSISGVGGGDII 140

Query: 133 NIFPDVNCSWESFKLIQKLLSKKNGEVVGDLMTPAPLVVSETMNFENAARLLLETKFHRL 192
           NIFPDVNCSWESFKLIQKLLSKKNGE+VGDLMTPAPLVVSETMNFENAARLLLETKFHRL
Sbjct: 141 NIFPDVNCSWESFKLIQKLLSKKNGEIVGDLMTPAPLVVSETMNFENAARLLLETKFHRL 200

Query: 193 PVVDCEGKLVGIITREDIVRVGLEMKRT 211
           PVVDCEGKLVGIITREDIVRVGLEMKRT
Sbjct: 201 PVVDCEGKLVGIITREDIVRVGLEMKRT 228

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BLAST of CSPI07G07500 vs. NCBI nr
Match: XP_038888405.1 (CBS domain-containing protein CBSX1, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 342.0 bits (876), Expect = 3.6e-90
Identity = 183/222 (82.43%), Postives = 192/222 (86.49%), Query Frame = 0


Query: 1   MASISTPYVPSVFPNSRLPTTQLR--HAG----------YRSPVVALAFSGHRVSSSIPF 60
           MASISTPYVPSV PNSRL  TQ R  +AG          +RSP VALAFSGHRV+SS  F
Sbjct: 1   MASISTPYVPSVLPNSRLLQTQFRLTYAGAGINSSPSSLFRSPAVALAFSGHRVASSSQF 60

Query: 61  RNGSYAVGDFMTKKGNLQVLKPSTSVEEALEVLVEKSLSGFPVVDDDWKLVGVVSDYDLL 120
           R GSY VGDFMTKKGNLQVLKPSTSV+EALEVLVEKSLSGFPVVDDDWKLVGVVSDYDLL
Sbjct: 61  RIGSYTVGDFMTKKGNLQVLKPSTSVDEALEVLVEKSLSGFPVVDDDWKLVGVVSDYDLL 120

Query: 121 ALDSISGVGGGDIINIFPDVNCSWESFKLIQKLLSKKNGEVVGDLMTPAPLVVSETMNFE 180
           ALDSISGVG  +  NIFPDVN SW+SFKLIQKLLSKKNGEVVGDLMTPAPLVV ETMN E
Sbjct: 121 ALDSISGVGDVE-ANIFPDVNSSWKSFKLIQKLLSKKNGEVVGDLMTPAPLVVRETMNLE 180

Query: 181 NAARLLLETKFHRLPVVDCEGKLVGIITREDIVRVGLEMKRT 211
           +AARLLLETKFH LPVVDCEGKL+GIITREDIVR GL+MKRT
Sbjct: 181 SAARLLLETKFHLLPVVDCEGKLMGIITREDIVRAGLQMKRT 221

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BLAST of CSPI07G07500 vs. NCBI nr
Match: KAA0031310.1 (CBS domain-containing protein CBSX2 [Cucumis melo var. makuwa])

HSP 1 Score: 340.9 bits (873), Expect = 8.1e-90
Identity = 184/220 (83.64%), Postives = 192/220 (87.27%), Query Frame = 0


Query: 1   MASISTPYVPSVFPNSRLPTTQLRHAG---------YRSPVVALAFSGHRVSSSIPFR-N 60
           MASISTPYVPS F NSRLPTTQ RHAG         +RSPV+ALAFSGHRV+SSIPFR N
Sbjct: 1   MASISTPYVPSFFSNSRLPTTQFRHAGTFDSSPSSLFRSPVLALAFSGHRVASSIPFRNN 60

Query: 61  GSYAVGDFMTKKGNLQVLKPSTSVEEALEVLVEKSLSGFPVVDDDWKLVGVVSDYDLLAL 120
           GSY VGDFMTKKGNL VLKPSTS+EEALEVLVEKS+SGFPVVDDDWKLVGVVSDYDLLAL
Sbjct: 61  GSYTVGDFMTKKGNLLVLKPSTSIEEALEVLVEKSVSGFPVVDDDWKLVGVVSDYDLLAL 120

Query: 121 DSISGVGGGDIINIFPDVNCSWESFKLIQKLLSKKNGEVVGDLMTPAPLVVSETMNFENA 180
           DSIS    G II +       W+SFKLIQKLLSKKNGE+VGDLMTPAPLVVSETMNFENA
Sbjct: 121 DSIS---VGLIIMLL------WQSFKLIQKLLSKKNGEIVGDLMTPAPLVVSETMNFENA 180

Query: 181 ARLLLETKFHRLPVVDCEGKLVGIITREDIVRVGLEMKRT 211
           ARLLLETKFHRLPVVDCEGKLVGIITREDIVRVGLEMKRT
Sbjct: 181 ARLLLETKFHRLPVVDCEGKLVGIITREDIVRVGLEMKRT 211

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BLAST of CSPI07G07500 vs. TAIR 10
Match: AT4G34120.1 (Cystathionine beta-synthase (CBS) family protein )

HSP 1 Score: 228.0 bits (580), Expect = 7.1e-60
Identity = 117/204 (57.35%), Postives = 154/204 (75.49%), Query Frame = 0


Query: 16  SRLPTTQLRHAGYRSPVVAL-AFSGHRVS----SSIPFRNGSYAVGDFMTKKGNLQVLKP 75
           S LP +  R +   SP + + AF     S    +S+P +NG Y VGDFMT + NL V+KP
Sbjct: 35  SLLPLSNRRRSSTFSPSITVSAFFAAPASVNNNNSVPAKNGGYTVGDFMTPRQNLHVVKP 94

Query: 76  STSVEEALEVLVEKSLSGFPVVDDDWKLVGVVSDYDLLALDSISGVGGGDIINIFPDVNC 135
           STSV++ALE+LVEK ++G PV+DD+W LVGVVSDYDLLALDSISG    D  N+FPDV+ 
Sbjct: 95  STSVDDALELLVEKKVTGLPVIDDNWTLVGVVSDYDLLALDSISGRSQND-TNLFPDVDS 154

Query: 136 SWESFKLIQKLLSKKNGEVVGDLMTPAPLVVSETMNFENAARLLLETKFHRLPVVDCEGK 195
           +W++F  +QKL+SK  G+VVGDLMTP+PLVV ++ N E+AARLLLETKF RLPVVD +GK
Sbjct: 155 TWKTFNELQKLISKTYGKVVGDLMTPSPLVVRDSTNLEDAARLLLETKFRRLPVVDADGK 214

Query: 196 LVGIITREDIVRVGLEMKRTQEDT 215
           L+GI+TR ++VR  L++KR  E++
Sbjct: 215 LIGILTRGNVVRAALQIKRETENS 237

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BLAST of CSPI07G07500 vs. TAIR 10
Match: AT4G36910.1 (Cystathionine beta-synthase (CBS) family protein )

HSP 1 Score: 219.5 bits (558), Expect = 2.5e-57
Identity = 115/214 (53.74%), Postives = 160/214 (74.77%), Query Frame = 0


Query: 2   ASISTPYV-----PSVFPNSRLPTTQLRHAGYRSPVVALAFSGHRVSSSIPFRNGSYAVG 61
           +S S+PY+      SV P  +   ++   +  R P  + A     +++S   R+G Y VG
Sbjct: 19  SSPSSPYLLLPRFLSVQPCHKFTFSRSFPSKSRIPSASSAAGSTLMTNSSSPRSGVYTVG 78

Query: 62  DFMTKKGNLQVLKPSTSVEEALEVLVEKSLSGFPVVDDDWKLVGVVSDYDLLALDSISGV 121
           +FMTKK +L V+KP+T+V+EALE+LVE  ++GFPV+D+DWKLVG+VSDYDLLALDSISG 
Sbjct: 79  EFMTKKEDLHVVKPTTTVDEALELLVENRITGFPVIDEDWKLVGLVSDYDLLALDSISGS 138

Query: 122 GGGDIINIFPDVNCSWESFKLIQKLLSKKNGEVVGDLMTPAPLVVSETMNFENAARLLLE 181
           G  +  ++FP+V+ +W++F  +QKLLSK NG++VGDLMTPAPLVV E  N E+AA++LLE
Sbjct: 139 GRTE-NSMFPEVDSTWKTFNAVQKLLSKTNGKLVGDLMTPAPLVVEEKTNLEDAAKILLE 198

Query: 182 TKFHRLPVVDCEGKLVGIITREDIVRVGLEMKRT 211
           TK+ RLPVVD +GKLVGIITR ++VR  L++KR+
Sbjct: 199 TKYRRLPVVDSDGKLVGIITRGNVVRAALQIKRS 231

The following BLAST results are available for this feature:

BLAST of CSPI07G07500 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9C5D0	1.0e-58	57.35	CBS domain-containing protein CBSX2, chloroplastic OS=Arabidopsis thaliana OX=37...	[more]
O23193	3.6e-56	53.74	CBS domain-containing protein CBSX1, chloroplastic OS=Arabidopsis thaliana OX=37...	[more]
O67820	1.5e-09	30.22	Inosine-5'-monophosphate dehydrogenase OS=Aquifex aeolicus (strain VF5) OX=22432...	[more]
Q9UY49	3.3e-09	28.36	Inosine-5'-monophosphate dehydrogenase OS=Pyrococcus abyssi (strain GE5 / Orsay)...	[more]
Q58821	7.3e-09	30.83	Uncharacterized protein MJ1426 OS=Methanocaldococcus jannaschii (strain ATCC 430...	[more]

BLAST of CSPI07G07500 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K7X9	3.2e-116	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G073520 PE=4 SV=1	[more]
A0A5D3C646	2.8e-104	90.45	CBS domain-containing protein CBSX2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5...	[more]
A0A1S3C189	3.0e-98	90.38	CBS domain-containing protein CBSX2, chloroplastic OS=Cucumis melo OX=3656 GN=LO...	[more]
A0A5A7SKP3	3.9e-90	83.64	CBS domain-containing protein CBSX2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6...	[more]
A0A6J1DN09	6.9e-79	73.18	CBS domain-containing protein CBSX1, chloroplastic-like OS=Momordica charantia O...	[more]

BLAST of CSPI07G07500 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004136971.1	6.6e-116	100.00	CBS domain-containing protein CBSX2, chloroplastic [Cucumis sativus] >KGN43916.1...	[more]
TYK06762.1	5.8e-104	90.45	CBS domain-containing protein CBSX2 [Cucumis melo var. makuwa]	[more]
XP_008455519.1	6.2e-98	90.38	PREDICTED: CBS domain-containing protein CBSX2, chloroplastic [Cucumis melo]	[more]
XP_038888405.1	3.6e-90	82.43	CBS domain-containing protein CBSX1, chloroplastic-like [Benincasa hispida]	[more]
KAA0031310.1	8.1e-90	83.64	CBS domain-containing protein CBSX2 [Cucumis melo var. makuwa]	[more]

BLAST of CSPI07G07500 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G34120.1	7.1e-60	57.35	Cystathionine beta-synthase (CBS) family protein	[more]
AT4G36910.1	2.5e-57	53.74	Cystathionine beta-synthase (CBS) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000644	CBS domain	SMART	SM00116	cbs_1	coord: 157..205 e-value: 3.1E-11 score: 53.3 coord: 64..112 e-value: 3.8E-8 score: 43.1
IPR000644	CBS domain	PFAM	PF00571	CBS	coord: 55..109 e-value: 2.9E-13 score: 50.0 coord: 150..203 e-value: 2.7E-15 score: 56.5
IPR000644	CBS domain	PROSITE	PS51371	CBS	coord: 59..121 score: 13.24678
IPR000644	CBS domain	PROSITE	PS51371	CBS	coord: 154..212 score: 14.495957
None	No IPR available	GENE3D	3.10.580.10		coord: 50..211 e-value: 5.6E-59 score: 200.3
None	No IPR available	PANTHER	PTHR48108	CBS DOMAIN-CONTAINING PROTEIN CBSX2, CHLOROPLASTIC	coord: 16..213
None	No IPR available	PANTHER	PTHR48108:SF8	CBS DOMAIN-CONTAINING PROTEIN CBSX1, CHLOROPLASTIC	coord: 16..213
None	No IPR available	SUPERFAMILY	54631	CBS-domain pair	coord: 49..202

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI07G07500.1	CSPI07G07500.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0045454	cell redox homeostasis