CSPI07G06350 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI07G06350
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	RING-type domain-containing protein
Location	Chr7: 4681846 .. 4682649 (-)
RNA-Seq Expression	CSPI07G06350
Synteny	CSPI07G06350

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCGAACAAATCTTTGGTCCTTGCAGGTATGGTTCTGGATCCTATATAGGTATGTCACTAAGAATTACTGATCCTGACCTAAGGCATCAACCACAAAGCCAAATTCGAACGCCACCCAACTCGCCAACACTTGTCAAACAAATCGATCTGAATTTCAAAGTTGAAGTCAAATGCCAACACTACTTTCAAGCAGGTGAAGGAGGCAACAGTACATTTGCAACAGCTTTACTTAGTGAACACATAAGTTCACATCATCCCTTGCCAACTTTGCAACTTCCTGTCTCCATCTTCAAGCATGGTGACAAAACTTTGAAGCGGTTACTTTTTCGGGAGTTTCAAATTTATAGAGCCATCATCAACATTGAACTTGTGGTTGACGAAATAATAAAACATTGGGTTAAAAAAGTAGATGAAGAGAATAGTACTTCTCAGGTGTTTGAAGAGATTTATCCTCTCGAAATTACGATAGAGTTGTTGGTTTATCAAATGATTCATGCAATAGACCAACCACATGAAATTATGATGGATCAACCACAAGTGATGATGGTTCCAACAAGCGAGAGTGCTATGGAGAGTATGCTGAAGAAAGTGGAAAACGAAGAAATAGTGAAATTTGGTGACAACAAAAGCATTAATTGTGTTATCTGTTTGGAAGAGATTAGTAAAGAGAAGAAAGGAAGTGAGGCAGTGGTGTTACAGATGCCATGCTTGCATATGTTTCATGAAGAATGCATAAGGAAGTGGTTGAAGAGAAGTCATTACTGTCCATCTTGTAGATTTTCAATGCCTACAAACAATTGA

mRNA sequence

ATGGCCGAACAAATCTTTGGTCCTTGCAGGTATGGTTCTGGATCCTATATAGGTATGTCACTAAGAATTACTGATCCTGACCTAAGGCATCAACCACAAAGCCAAATTCGAACGCCACCCAACTCGCCAACACTTGTCAAACAAATCGATCTGAATTTCAAAGTTGAAGTCAAATGCCAACACTACTTTCAAGCAGGTGAAGGAGGCAACAGTACATTTGCAACAGCTTTACTTAGTGAACACATAAGTTCACATCATCCCTTGCCAACTTTGCAACTTCCTGTCTCCATCTTCAAGCATGGTGACAAAACTTTGAAGCGGTTACTTTTTCGGGAGTTTCAAATTTATAGAGCCATCATCAACATTGAACTTGTGGTTGACGAAATAATAAAACATTGGGTTAAAAAAGTAGATGAAGAGAATAGTACTTCTCAGGTGTTTGAAGAGATTTATCCTCTCGAAATTACGATAGAGTTGTTGGTTTATCAAATGATTCATGCAATAGACCAACCACATGAAATTATGATGGATCAACCACAAGTGATGATGGTTCCAACAAGCGAGAGTGCTATGGAGAGTATGCTGAAGAAAGTGGAAAACGAAGAAATAGTGAAATTTGGTGACAACAAAAGCATTAATTGTGTTATCTGTTTGGAAGAGATTAGTAAAGAGAAGAAAGGAAGTGAGGCAGTGGTGTTACAGATGCCATGCTTGCATATGTTTCATGAAGAATGCATAAGGAAGTGGTTGAAGAGAAGTCATTACTGTCCATCTTGTAGATTTTCAATGCCTACAAACAATTGA

Coding sequence (CDS)

ATGGCCGAACAAATCTTTGGTCCTTGCAGGTATGGTTCTGGATCCTATATAGGTATGTCACTAAGAATTACTGATCCTGACCTAAGGCATCAACCACAAAGCCAAATTCGAACGCCACCCAACTCGCCAACACTTGTCAAACAAATCGATCTGAATTTCAAAGTTGAAGTCAAATGCCAACACTACTTTCAAGCAGGTGAAGGAGGCAACAGTACATTTGCAACAGCTTTACTTAGTGAACACATAAGTTCACATCATCCCTTGCCAACTTTGCAACTTCCTGTCTCCATCTTCAAGCATGGTGACAAAACTTTGAAGCGGTTACTTTTTCGGGAGTTTCAAATTTATAGAGCCATCATCAACATTGAACTTGTGGTTGACGAAATAATAAAACATTGGGTTAAAAAAGTAGATGAAGAGAATAGTACTTCTCAGGTGTTTGAAGAGATTTATCCTCTCGAAATTACGATAGAGTTGTTGGTTTATCAAATGATTCATGCAATAGACCAACCACATGAAATTATGATGGATCAACCACAAGTGATGATGGTTCCAACAAGCGAGAGTGCTATGGAGAGTATGCTGAAGAAAGTGGAAAACGAAGAAATAGTGAAATTTGGTGACAACAAAAGCATTAATTGTGTTATCTGTTTGGAAGAGATTAGTAAAGAGAAGAAAGGAAGTGAGGCAGTGGTGTTACAGATGCCATGCTTGCATATGTTTCATGAAGAATGCATAAGGAAGTGGTTGAAGAGAAGTCATTACTGTCCATCTTGTAGATTTTCAATGCCTACAAACAATTGA

Protein sequence

MAEQIFGPCRYGSGSYIGMSLRITDPDLRHQPQSQIRTPPNSPTLVKQIDLNFKVEVKCQHYFQAGEGGNSTFATALLSEHISSHHPLPTLQLPVSIFKHGDKTLKRLLFREFQIYRAIINIELVVDEIIKHWVKKVDEENSTSQVFEEIYPLEITIELLVYQMIHAIDQPHEIMMDQPQVMMVPTSESAMESMLKKVENEEIVKFGDNKSINCVICLEEISKEKKGSEAVVLQMPCLHMFHEECIRKWLKRSHYCPSCRFSMPTNN*

Homology

[Close]

BLAST of CSPI07G06350 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 1.5e-08
Identity = 29/83 (34.94%), Postives = 53/83 (63.86%), Query Frame = 0


Query: 185 PTSESAMESMLKKVENEEIVKFGDNKSINCVICLEEISKEKKGSEAVVLQMPCLHMFHEE 244
           P S+SA++++      ++++K   N+   C +C++E    + GS+  V QMPC H+FH++
Sbjct: 190 PASKSAIDALPTVKVTKDMLKSEMNQ---CAVCMDEF---EDGSD--VKQMPCKHVFHQD 249

Query: 245 CIRKWLKRSHYCPSCRFSMPTNN 268
           C+  WL+  + CP CRF +PT++
Sbjct: 250 CLLPWLELHNSCPVCRFELPTDD 264

[Close]

BLAST of CSPI07G06350 vs. ExPASy Swiss-Prot
Match: Q940T5 (E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 1.0e-07
Identity = 30/81 (37.04%), Postives = 41/81 (50.62%), Query Frame = 0


Query: 185 PTSESAMESMLKKVENEEIVKFGDNKSINCVICLEEISKEKKGSEAVVLQMPCLHMFHEE 244
           P S+SA+ES+       EI         NC +C E    E +  E     MPC H+FH++
Sbjct: 174 PASKSAIESL----PRVEISDCHIGSEANCAVCTEIFETETEARE-----MPCKHLFHDD 233

Query: 245 CIRKWLKRSHYCPSCRFSMPT 266
           CI  WL   + CP CRF +P+
Sbjct: 234 CIVPWLSIRNSCPVCRFELPS 245

[Close]

BLAST of CSPI07G06350 vs. ExPASy Swiss-Prot
Match: Q3T0W3 (E3 ubiquitin-protein ligase RNF181 OS=Bos taurus OX=9913 GN=RNF181 PE=2 SV=1)

HSP 1 Score: 58.2 bits (139), Expect = 1.7e-07
Identity = 27/94 (28.72%), Postives = 51/94 (54.26%), Query Frame = 0


Query: 174 IMMDQPQVMMVPTSESAMESMLKKVENEEIVKFGDNKSINCVICLEEISKEKKGSEAVVL 233
           +++D    +  P +++A+E++ + V        G    + C +CL E  +E+       +
Sbjct: 42  LVVDWDHHLPPPAAKTAVENLPRTVIR------GSQAELKCPVCLLEFEEEE-----TAI 101

Query: 234 QMPCLHMFHEECIRKWLKRSHYCPSCRFSMPTNN 268
           +MPC H+FH  CI  WL +++ CP CR  +PT++
Sbjct: 102 EMPCHHLFHSNCILPWLSKTNSCPLCRHELPTDD 124

[Close]

BLAST of CSPI07G06350 vs. ExPASy Swiss-Prot
Match: Q9P0P0 (E3 ubiquitin-protein ligase RNF181 OS=Homo sapiens OX=9606 GN=RNF181 PE=1 SV=1)

HSP 1 Score: 58.2 bits (139), Expect = 1.7e-07
Identity = 26/94 (27.66%), Postives = 51/94 (54.26%), Query Frame = 0


Query: 174 IMMDQPQVMMVPTSESAMESMLKKVENEEIVKFGDNKSINCVICLEEISKEKKGSEAVVL 233
           +++D    +  P +++ +E++ + V        G    + C +CL E  +E+       +
Sbjct: 42  LVVDWDHHLPPPAAKTVVENLPRTVIR------GSQAELKCPVCLLEFEEEE-----TAI 101

Query: 234 QMPCLHMFHEECIRKWLKRSHYCPSCRFSMPTNN 268
           +MPC H+FH  CI  WL +++ CP CR+ +PT++
Sbjct: 102 EMPCHHLFHSSCILPWLSKTNSCPLCRYELPTDD 124

[Close]

BLAST of CSPI07G06350 vs. ExPASy Swiss-Prot
Match: Q9LQX2 (E3 ubiquitin-protein ligase MPSR1 OS=Arabidopsis thaliana OX=3702 GN=MPSR1 PE=1 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 2.9e-07
Identity = 27/53 (50.94%), Postives = 30/53 (56.60%), Query Frame = 0


Query: 214 CVICLEEISKEKKGSEAVVLQMPCLHMFHEECIRKWLKRSHYCPSCRFSMPTN 267
           CVICLEE       SE  V +MPC H FH  CI KWL     CP CR+ MP +
Sbjct: 113 CVICLEEWK-----SEETVKEMPCKHRFHGGCIEKWLGFHGSCPVCRYEMPVD 160

[Close]

BLAST of CSPI07G06350 vs. ExPASy TrEMBL
Match: A0A0A0K7L0 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G067560 PE=4 SV=1)

HSP 1 Score: 542.0 bits (1395), Expect = 1.4e-150
Identity = 266/267 (99.63%), Postives = 267/267 (100.00%), Query Frame = 0


Query: 1   MAEQIFGPCRYGSGSYIGMSLRITDPDLRHQPQSQIRTPPNSPTLVKQIDLNFKVEVKCQ 60
           MAEQIFGPCRYGSGSYIGMSLRITDPDLRHQPQSQIRTPPNSPTLVKQIDLNFKVEVKCQ
Sbjct: 1   MAEQIFGPCRYGSGSYIGMSLRITDPDLRHQPQSQIRTPPNSPTLVKQIDLNFKVEVKCQ 60

Query: 61  HYFQAGEGGNSTFATALLSEHISSHHPLPTLQLPVSIFKHGDKTLKRLLFREFQIYRAII 120
           HYFQAGEGGNSTFATALLSEHISSHHPLPTLQLPVSIFKHGDKTLKRLLFREFQIYRAII
Sbjct: 61  HYFQAGEGGNSTFATALLSEHISSHHPLPTLQLPVSIFKHGDKTLKRLLFREFQIYRAII 120

Query: 121 NIELVVDEIIKHWVKKVDEENSTSQVFEEIYPLEITIELLVYQMIHAIDQPHEIMMDQPQ 180
           NIELVVDEIIKHWVKKVDEENSTSQVFEEIYPLEITIELLVYQMIHAIDQPHEIMMDQPQ
Sbjct: 121 NIELVVDEIIKHWVKKVDEENSTSQVFEEIYPLEITIELLVYQMIHAIDQPHEIMMDQPQ 180

Query: 181 VMMVPTSESAMESMLKKVENEEIVKFGDNKSINCVICLEEISKEKKGSEAVVLQMPCLHM 240
           VMMVPTSESAMESMLKKVENEEIVKFGDNKSINCVICLEEISKEKKGSEAVVLQMPCLHM
Sbjct: 181 VMMVPTSESAMESMLKKVENEEIVKFGDNKSINCVICLEEISKEKKGSEAVVLQMPCLHM 240

Query: 241 FHEECIRKWLKRSHYCPSCRFSMPTNN 268
           FHEECIRKWLKRSHYCP+CRFSMPTNN
Sbjct: 241 FHEECIRKWLKRSHYCPTCRFSMPTNN 267

[Close]

BLAST of CSPI07G06350 vs. ExPASy TrEMBL
Match: A0A0A0K493 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G067540 PE=4 SV=1)

HSP 1 Score: 380.2 bits (975), Expect = 7.2e-102
Identity = 195/268 (72.76%), Postives = 223/268 (83.21%), Query Frame = 0


Query: 1   MAEQIFGPCRYGSGSYIGMSLRITDPDLRHQPQSQIRTPPNSPTLVKQIDLNFKVEVKCQ 60
           MAE  F  C   +G ++GMSLRITDPDL  QPQSQ +TP NSPT VKQI+L  KVEVKCQ
Sbjct: 1   MAEHNFDDC-MSTGCFMGMSLRITDPDLSLQPQSQNQTPHNSPTFVKQINLKLKVEVKCQ 60

Query: 61  HYFQAGEGGNSTFATALLSEHISSHHPLPTLQLPVSIFKHGDKTLKRLLFREFQIYRAII 120
           H FQA E G+ TFA+ L+ EHI SHHP PT QLPVS+F+HG++TLKRLLF++FQI+R II
Sbjct: 61  H-FQADEEGD-TFASTLMIEHIVSHHPFPTFQLPVSVFRHGNRTLKRLLFQQFQIFRGII 120

Query: 121 NIELVVDEIIKHWVKK-VDEENSTSQVFEEIYPLEITIELLVYQMIHAIDQPHEIMMDQP 180
           NI+LV DEII+HWVKK  D EN++  + EEIYPLEITIELL+ +MIHAIDQP E  +DQP
Sbjct: 121 NIQLVADEIIEHWVKKEEDRENNSGVLLEEIYPLEITIELLLLRMIHAIDQPEE-TIDQP 180

Query: 181 QVMMVPTSESAMESMLKKVENEEIVKFGDNKSINCVICLEEISKEKKGSEAVVLQMPCLH 240
           QV MVP SESAMESMLK+VE EEIVK G++KSINCVICLEEISK+ KGSE VVLQMPCLH
Sbjct: 181 QVTMVPASESAMESMLKRVEKEEIVKVGEDKSINCVICLEEISKKMKGSEGVVLQMPCLH 240

Query: 241 MFHEECIRKWLKRSHYCPSCRFSMPTNN 268
           MFHEECIR WLK SHYCP+CRFSMP NN
Sbjct: 241 MFHEECIRTWLKTSHYCPTCRFSMPINN 264

[Close]

BLAST of CSPI07G06350 vs. ExPASy TrEMBL
Match: A0A0A0K2A1 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G067550 PE=4 SV=1)

HSP 1 Score: 375.2 bits (962), Expect = 2.3e-100
Identity = 199/268 (74.25%), Postives = 223/268 (83.21%), Query Frame = 0


Query: 1   MAEQIFGPCRYGSGSYIGMSLRITDPDLRHQPQSQIRTPPNSPTLVKQIDLNFKVEVKCQ 60
           MAEQ F  C   SG Y+GMSLRITDP         I+T  NSPT VKQIDLNFKVEVKCQ
Sbjct: 1   MAEQNFDDCT--SGYYMGMSLRITDP---------IQTRSNSPTSVKQIDLNFKVEVKCQ 60

Query: 61  HYFQAGEGGNSTFATALLSEHISSHHPLPTLQLPVSIFKHGDKTLKRLLFREFQIYRAII 120
           H FQAGE  ++TFAT  LSEHI +H P P LQLPVSIFKHGDKTLKRLLF++FQ++RAII
Sbjct: 61  H-FQAGE-EDTTFATTFLSEHIIAHQPFPALQLPVSIFKHGDKTLKRLLFQKFQMFRAII 120

Query: 121 NIELVVDEIIKHWVKKV-DEENSTSQVFEEIYPLEITIELLVYQMIHAIDQPHEIMMDQP 180
           NIELV D+IIKHWVKKV D++ + S VFEEIYPLEITIE+LV QMI A+DQP E  +DQP
Sbjct: 121 NIELVTDQIIKHWVKKVEDDKENNSGVFEEIYPLEITIEVLVLQMIDAVDQPQE-TIDQP 180

Query: 181 QVMMVPTSESAMESMLKKVENEEIVKFGDNKSINCVICLEEISKEKKGSEAVVLQMPCLH 240
           QV MVPTS+SAM+SMLK+VENEEI+K GD KSI+CVICLE+ISKE+KGSE VVLQMPCLH
Sbjct: 181 QVRMVPTSDSAMKSMLKRVENEEIMKVGDYKSISCVICLEKISKEEKGSEGVVLQMPCLH 240

Query: 241 MFHEECIRKWLKRSHYCPSCRFSMPTNN 268
           MFHEECIRKWLK SHYCP+CRFSM  NN
Sbjct: 241 MFHEECIRKWLKTSHYCPTCRFSMTINN 254

[Close]

BLAST of CSPI07G06350 vs. ExPASy TrEMBL
Match: A0A5D3C4K6 (Putative zinc finger protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G001670 PE=4 SV=1)

HSP 1 Score: 361.7 bits (927), Expect = 2.7e-96
Identity = 186/271 (68.63%), Postives = 224/271 (82.66%), Query Frame = 0


Query: 1   MAEQIFGPCRYGSGSYIGMSLRITDPDLRHQPQSQIRTPPNSPTLVKQIDLNFKVEVKCQ 60
           MAE+IF  CRYGSGSY+GMSLRITDP+ RHQP +QI    +SPTLV+QI L FKV++KCQ
Sbjct: 1   MAEEIFNICRYGSGSYMGMSLRITDPNPRHQPLNQI-DQTSSPTLVEQICLKFKVQLKCQ 60

Query: 61  HYFQAGEGGNSTFATALLSEHISSHHPLPTLQLPVSIFKHGDKTLKRLLFREFQIYRAII 120
           H FQ  EGGN   +T L+ EHI S H   T QLP+SIFKHGDKTLKR+LFREF++Y  II
Sbjct: 61  H-FQVWEGGNG-HSTTLVREHIRSQHSFATFQLPISIFKHGDKTLKRILFREFKMYGDII 120

Query: 121 NIELVVDEIIKHWVKKV----DEENSTSQVFEEIYPLEITIELLVYQMIHAIDQPHEIMM 180
           NIEL+ +EIIKHWV+KV    D+EN+++ +F++IYPLEI I LLV +MI A+ QP   M+
Sbjct: 121 NIELLANEIIKHWVRKVEEEEDQENNSNVLFKKIYPLEIMIRLLVSKMIRAV-QPRLAMI 180

Query: 181 DQPQVMMVPTSESAMESMLKKVENEEIVKFGDNKSINCVICLEEISKEKKGSEAVVLQMP 240
           DQP  +MVPTS+SA+ESMLK+VENEEI+K GD++SINCV+CLEEISKE+KGSE  VLQMP
Sbjct: 181 DQP--LMVPTSDSAVESMLKRVENEEIMKSGDDESINCVVCLEEISKEEKGSETTVLQMP 240

Query: 241 CLHMFHEECIRKWLKRSHYCPSCRFSMPTNN 268
           CLHMFHEECIRKWLK SHYCP+CRFSMP +N
Sbjct: 241 CLHMFHEECIRKWLKTSHYCPTCRFSMPIDN 265

[Close]

BLAST of CSPI07G06350 vs. ExPASy TrEMBL
Match: E5GCQ3 (Hypothetical zinc finger protein OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 361.7 bits (927), Expect = 2.7e-96
Identity = 186/271 (68.63%), Postives = 224/271 (82.66%), Query Frame = 0


Query: 1   MAEQIFGPCRYGSGSYIGMSLRITDPDLRHQPQSQIRTPPNSPTLVKQIDLNFKVEVKCQ 60
           MAE+IF  CRYGSGSY+GMSLRITDP+ RHQP +QI    +SPTLV+QI L FKV++KCQ
Sbjct: 1   MAEEIFNICRYGSGSYMGMSLRITDPNPRHQPLNQI-DQTSSPTLVEQICLKFKVQLKCQ 60

Query: 61  HYFQAGEGGNSTFATALLSEHISSHHPLPTLQLPVSIFKHGDKTLKRLLFREFQIYRAII 120
           H FQ  EGGN   +T L+ EHI S H   T QLP+SIFKHGDKTLKR+LFREF++Y  II
Sbjct: 61  H-FQVWEGGNG-HSTTLVREHIRSQHSFATFQLPISIFKHGDKTLKRILFREFKMYGDII 120

Query: 121 NIELVVDEIIKHWVKKV----DEENSTSQVFEEIYPLEITIELLVYQMIHAIDQPHEIMM 180
           NIEL+ +EIIKHWV+KV    D+EN+++ +F++IYPLEI I LLV +MI A+ QP   M+
Sbjct: 121 NIELLANEIIKHWVRKVEEEEDQENNSNVLFKKIYPLEIMIRLLVSKMIRAV-QPRLAMI 180

Query: 181 DQPQVMMVPTSESAMESMLKKVENEEIVKFGDNKSINCVICLEEISKEKKGSEAVVLQMP 240
           DQP  +MVPTS+SA+ESMLK+VENEEI+K GD++SINCV+CLEEISKE+KGSE  VLQMP
Sbjct: 181 DQP--LMVPTSDSAVESMLKRVENEEIMKSGDDESINCVVCLEEISKEEKGSETTVLQMP 240

Query: 241 CLHMFHEECIRKWLKRSHYCPSCRFSMPTNN 268
           CLHMFHEECIRKWLK SHYCP+CRFSMP +N
Sbjct: 241 CLHMFHEECIRKWLKTSHYCPTCRFSMPIDN 265

[Close]

BLAST of CSPI07G06350 vs. NCBI nr
Match: KAE8645828.1 (hypothetical protein Csa_017274 [Cucumis sativus])

HSP 1 Score: 521.2 bits (1341), Expect = 5.4e-144
Identity = 257/258 (99.61%), Postives = 258/258 (100.00%), Query Frame = 0


Query: 1   MAEQIFGPCRYGSGSYIGMSLRITDPDLRHQPQSQIRTPPNSPTLVKQIDLNFKVEVKCQ 60
           MAEQIFGPCRYGSGSYIGMSLRITDPDLRHQPQSQIRTPPNSPTLVKQIDLNFKVEVKCQ
Sbjct: 1   MAEQIFGPCRYGSGSYIGMSLRITDPDLRHQPQSQIRTPPNSPTLVKQIDLNFKVEVKCQ 60

Query: 61  HYFQAGEGGNSTFATALLSEHISSHHPLPTLQLPVSIFKHGDKTLKRLLFREFQIYRAII 120
           HYFQAGEGGNSTFATALLSEHISSHHPLPTLQLPVSIFKHGDKTLKRLLFREFQIYRAII
Sbjct: 61  HYFQAGEGGNSTFATALLSEHISSHHPLPTLQLPVSIFKHGDKTLKRLLFREFQIYRAII 120

Query: 121 NIELVVDEIIKHWVKKVDEENSTSQVFEEIYPLEITIELLVYQMIHAIDQPHEIMMDQPQ 180
           NIELVVDEIIKHWVKKVDEENSTSQVFEEIYPLEITIELLVYQMIHAIDQPHEIMMDQPQ
Sbjct: 121 NIELVVDEIIKHWVKKVDEENSTSQVFEEIYPLEITIELLVYQMIHAIDQPHEIMMDQPQ 180

Query: 181 VMMVPTSESAMESMLKKVENEEIVKFGDNKSINCVICLEEISKEKKGSEAVVLQMPCLHM 240
           VMMVPTSESAMESMLKKVENEEIVKFGDNKSINCVICLEEISKEKKGSEAVVLQMPCLHM
Sbjct: 181 VMMVPTSESAMESMLKKVENEEIVKFGDNKSINCVICLEEISKEKKGSEAVVLQMPCLHM 240

Query: 241 FHEECIRKWLKRSHYCPS 259
           FHEECIRKWLKRSHYCP+
Sbjct: 241 FHEECIRKWLKRSHYCPT 258

[Close]

BLAST of CSPI07G06350 vs. NCBI nr
Match: KGN43799.1 (hypothetical protein Csa_017222 [Cucumis sativus])

HSP 1 Score: 380.2 bits (975), Expect = 1.5e-101
Identity = 195/268 (72.76%), Postives = 223/268 (83.21%), Query Frame = 0


Query: 1   MAEQIFGPCRYGSGSYIGMSLRITDPDLRHQPQSQIRTPPNSPTLVKQIDLNFKVEVKCQ 60
           MAE  F  C   +G ++GMSLRITDPDL  QPQSQ +TP NSPT VKQI+L  KVEVKCQ
Sbjct: 1   MAEHNFDDC-MSTGCFMGMSLRITDPDLSLQPQSQNQTPHNSPTFVKQINLKLKVEVKCQ 60

Query: 61  HYFQAGEGGNSTFATALLSEHISSHHPLPTLQLPVSIFKHGDKTLKRLLFREFQIYRAII 120
           H FQA E G+ TFA+ L+ EHI SHHP PT QLPVS+F+HG++TLKRLLF++FQI+R II
Sbjct: 61  H-FQADEEGD-TFASTLMIEHIVSHHPFPTFQLPVSVFRHGNRTLKRLLFQQFQIFRGII 120

Query: 121 NIELVVDEIIKHWVKK-VDEENSTSQVFEEIYPLEITIELLVYQMIHAIDQPHEIMMDQP 180
           NI+LV DEII+HWVKK  D EN++  + EEIYPLEITIELL+ +MIHAIDQP E  +DQP
Sbjct: 121 NIQLVADEIIEHWVKKEEDRENNSGVLLEEIYPLEITIELLLLRMIHAIDQPEE-TIDQP 180

Query: 181 QVMMVPTSESAMESMLKKVENEEIVKFGDNKSINCVICLEEISKEKKGSEAVVLQMPCLH 240
           QV MVP SESAMESMLK+VE EEIVK G++KSINCVICLEEISK+ KGSE VVLQMPCLH
Sbjct: 181 QVTMVPASESAMESMLKRVEKEEIVKVGEDKSINCVICLEEISKKMKGSEGVVLQMPCLH 240

Query: 241 MFHEECIRKWLKRSHYCPSCRFSMPTNN 268
           MFHEECIR WLK SHYCP+CRFSMP NN
Sbjct: 241 MFHEECIRTWLKTSHYCPTCRFSMPINN 264

[Close]

BLAST of CSPI07G06350 vs. NCBI nr
Match: ADN34252.1 (hypothetical zinc finger protein [Cucumis melo subsp. melo] >KAA0031412.1 putative zinc finger protein [Cucumis melo var. makuwa] >TYK06863.1 putative zinc finger protein [Cucumis melo var. makuwa])

HSP 1 Score: 361.7 bits (927), Expect = 5.5e-96
Identity = 186/271 (68.63%), Postives = 224/271 (82.66%), Query Frame = 0


Query: 1   MAEQIFGPCRYGSGSYIGMSLRITDPDLRHQPQSQIRTPPNSPTLVKQIDLNFKVEVKCQ 60
           MAE+IF  CRYGSGSY+GMSLRITDP+ RHQP +QI    +SPTLV+QI L FKV++KCQ
Sbjct: 1   MAEEIFNICRYGSGSYMGMSLRITDPNPRHQPLNQI-DQTSSPTLVEQICLKFKVQLKCQ 60

Query: 61  HYFQAGEGGNSTFATALLSEHISSHHPLPTLQLPVSIFKHGDKTLKRLLFREFQIYRAII 120
           H FQ  EGGN   +T L+ EHI S H   T QLP+SIFKHGDKTLKR+LFREF++Y  II
Sbjct: 61  H-FQVWEGGNG-HSTTLVREHIRSQHSFATFQLPISIFKHGDKTLKRILFREFKMYGDII 120

Query: 121 NIELVVDEIIKHWVKKV----DEENSTSQVFEEIYPLEITIELLVYQMIHAIDQPHEIMM 180
           NIEL+ +EIIKHWV+KV    D+EN+++ +F++IYPLEI I LLV +MI A+ QP   M+
Sbjct: 121 NIELLANEIIKHWVRKVEEEEDQENNSNVLFKKIYPLEIMIRLLVSKMIRAV-QPRLAMI 180

Query: 181 DQPQVMMVPTSESAMESMLKKVENEEIVKFGDNKSINCVICLEEISKEKKGSEAVVLQMP 240
           DQP  +MVPTS+SA+ESMLK+VENEEI+K GD++SINCV+CLEEISKE+KGSE  VLQMP
Sbjct: 181 DQP--LMVPTSDSAVESMLKRVENEEIMKSGDDESINCVVCLEEISKEEKGSETTVLQMP 240

Query: 241 CLHMFHEECIRKWLKRSHYCPSCRFSMPTNN 268
           CLHMFHEECIRKWLK SHYCP+CRFSMP +N
Sbjct: 241 CLHMFHEECIRKWLKTSHYCPTCRFSMPIDN 265

[Close]

BLAST of CSPI07G06350 vs. NCBI nr
Match: ADN34253.1 (hypothetical zinc finger protein [Cucumis melo subsp. melo] >TYK06864.1 putative zinc finger protein [Cucumis melo var. makuwa])

HSP 1 Score: 354.0 bits (907), Expect = 1.1e-93
Identity = 192/271 (70.85%), Postives = 223/271 (82.29%), Query Frame = 0


Query: 1   MAEQIFGPCRYGSGSYIGMSLRITDPDLRH-QPQSQIRTPPNSPTLVKQIDLNFKVEVKC 60
           MAEQ    C   +G Y+GMSLRITDPDLRH QPQSQI+T PNSP LVKQIDLNFK++VKC
Sbjct: 1   MAEQNLDAC-MSAGYYMGMSLRITDPDLRHRQPQSQIQTQPNSPALVKQIDLNFKLQVKC 60

Query: 61  QHYFQAGEGGNSTFATALLSEHISSHHPLPTLQLPVSIFKHGDKTLKRLLFREFQIYRAI 120
           QH F AGE G STFAT LLSEHI S    P LQLP+SIFK GDKTLKRLLF++FQ++R I
Sbjct: 61  QH-FGAGEEG-STFATTLLSEHIISQPTFPALQLPISIFKRGDKTLKRLLFQKFQMFRGI 120

Query: 121 INIELVVDEIIKHWVKKVDE-ENSTSQVFEEIYPLEITIELLVYQMIHAIDQPHEIMMDQ 180
           +N+ELVVDEIIKHW++KV+E + + S+VF EIYPLEI IELLV +MI AI       +DQ
Sbjct: 121 VNVELVVDEIIKHWIRKVEEDQENNSRVFAEIYPLEIRIELLVCRMIPAI-------IDQ 180

Query: 181 PQ-VMMVPTSESAMESMLKKVE-NEEIVKFGDNKSINCVICLEEISKEKKGSEAVVLQMP 240
           PQ VMMVPTS++AMESMLK+VE NEE++K GD+ SI+CVICLE+I KE+K S  VVLQMP
Sbjct: 181 PQEVMMVPTSDNAMESMLKRVENNEELMKLGDD-SIDCVICLEKIGKEEKRSGRVVLQMP 240

Query: 241 CLHMFHEECIRKWLKRSHYCPSCRFSMPTNN 268
           CLHMFHEECIRKWLK SH+CP+CRFSMP NN
Sbjct: 241 CLHMFHEECIRKWLKTSHFCPTCRFSMPINN 260

[Close]

BLAST of CSPI07G06350 vs. NCBI nr
Match: KAA0031413.1 (putative zinc finger protein [Cucumis melo var. makuwa])

HSP 1 Score: 352.8 bits (904), Expect = 2.6e-93
Identity = 191/271 (70.48%), Postives = 224/271 (82.66%), Query Frame = 0


Query: 1   MAEQIFGPCRYGSGSYIGMSLRITDPDLRH-QPQSQIRTPPNSPTLVKQIDLNFKVEVKC 60
           MAEQ    C   +G Y+GMSLRITDPDLRH QPQSQI+T PNSP LVKQIDLNFK++VKC
Sbjct: 1   MAEQNLDAC-MSAGYYMGMSLRITDPDLRHRQPQSQIQTQPNSPALVKQIDLNFKLQVKC 60

Query: 61  QHYFQAGEGGNSTFATALLSEHISSHHPLPTLQLPVSIFKHGDKTLKRLLFREFQIYRAI 120
           QH F AGE G STFAT LLSEHI S    P LQLP+SIFK GDKTLKRLLF++FQ++R I
Sbjct: 61  QH-FGAGEEG-STFATTLLSEHIISQPTFPALQLPISIFKRGDKTLKRLLFQKFQMFRGI 120

Query: 121 INIELVVDEIIKHWVKKVDE-ENSTSQVFEEIYPLEITIELLVYQMIHAIDQPHEIMMDQ 180
           +N+ELVVDEIIKHW++KV+E + ++S+VF EIYPLEI IELLV +MI AI       +DQ
Sbjct: 121 VNVELVVDEIIKHWIRKVEEDQENSSRVFAEIYPLEIRIELLVCRMIPAI-------IDQ 180

Query: 181 PQ-VMMVPTSESAMESMLKKVE-NEEIVKFGDNKSINCVICLEEISKEKKGSEAVVLQMP 240
           PQ VMMVPTS++AMESMLK+VE N+E++K GD+ SI+CVICLE+I KE+K S  VVLQMP
Sbjct: 181 PQEVMMVPTSDNAMESMLKRVENNKELMKLGDD-SIDCVICLEKIGKEEKRSGRVVLQMP 240

Query: 241 CLHMFHEECIRKWLKRSHYCPSCRFSMPTNN 268
           CLHMFHEECIRKWLK SH+CP+CRFSMP NN
Sbjct: 241 CLHMFHEECIRKWLKTSHFCPTCRFSMPINN 260

[Close]

BLAST of CSPI07G06350 vs. TAIR 10
Match: AT3G19950.1 (RING/U-box superfamily protein )

HSP 1 Score: 61.6 bits (148), Expect = 1.1e-09
Identity = 29/83 (34.94%), Postives = 53/83 (63.86%), Query Frame = 0


Query: 185 PTSESAMESMLKKVENEEIVKFGDNKSINCVICLEEISKEKKGSEAVVLQMPCLHMFHEE 244
           P S+SA++++      ++++K   N+   C +C++E    + GS+  V QMPC H+FH++
Sbjct: 190 PASKSAIDALPTVKVTKDMLKSEMNQ---CAVCMDEF---EDGSD--VKQMPCKHVFHQD 249

Query: 245 CIRKWLKRSHYCPSCRFSMPTNN 268
           C+  WL+  + CP CRF +PT++
Sbjct: 250 CLLPWLELHNSCPVCRFELPTDD 264

[Close]

BLAST of CSPI07G06350 vs. TAIR 10
Match: AT5G37270.1 (RING/U-box superfamily protein )

HSP 1 Score: 60.1 bits (144), Expect = 3.2e-09
Identity = 28/82 (34.15%), Postives = 46/82 (56.10%), Query Frame = 0


Query: 182 MMVPTSESAMESMLKKVENEEIVKF---GDNKSINCVICLEEISKEKKGSEAVVLQMPCL 241
           +M+P+       M +++  E+ ++F   GD +   C ICLE+ S+     + ++L   C 
Sbjct: 118 VMLPSIAVPSRDMFQRLLEEQTMEFTDLGDEEETTCSICLEDFSESH--DDNIILLPDCF 177

Query: 242 HMFHEECIRKWLKRSHYCPSCR 261
           H+FH+ CI +WLKR   CP CR
Sbjct: 178 HLFHQSCIFEWLKRQRSCPLCR 197

[Close]

BLAST of CSPI07G06350 vs. TAIR 10
Match: AT5G37230.1 (RING/U-box superfamily protein )

HSP 1 Score: 59.7 bits (143), Expect = 4.2e-09
Identity = 28/82 (34.15%), Postives = 44/82 (53.66%), Query Frame = 0


Query: 182 MMVPTSESAMESMLKKVENE---EIVKFGDNKSINCVICLEEISKEKKGSEAVVLQMPCL 241
           +M+P+       M +++  E   E+   GD +   C IC+E+ S+     + ++L   C 
Sbjct: 118 VMLPSIVVRSRDMFQRLLEEQTMELTNLGDEEETTCSICMEDFSESH--DDNIILLPDCF 177

Query: 242 HMFHEECIRKWLKRSHYCPSCR 261
           H+FH+ CI KWLKR   CP CR
Sbjct: 178 HLFHQSCIFKWLKRQRSCPLCR 197

[Close]

BLAST of CSPI07G06350 vs. TAIR 10
Match: AT5G59550.1 (zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 58.9 bits (141), Expect = 7.1e-09
Identity = 30/81 (37.04%), Postives = 41/81 (50.62%), Query Frame = 0


Query: 185 PTSESAMESMLKKVENEEIVKFGDNKSINCVICLEEISKEKKGSEAVVLQMPCLHMFHEE 244
           P S+SA+ES+       EI         NC +C E    E +  E     MPC H+FH++
Sbjct: 174 PASKSAIESL----PRVEISDCHIGSEANCAVCTEIFETETEARE-----MPCKHLFHDD 233

Query: 245 CIRKWLKRSHYCPSCRFSMPT 266
           CI  WL   + CP CRF +P+
Sbjct: 234 CIVPWLSIRNSCPVCRFELPS 245

[Close]

BLAST of CSPI07G06350 vs. TAIR 10
Match: AT5G60820.1 (RING/U-box superfamily protein )

HSP 1 Score: 57.8 bits (138), Expect = 1.6e-08
Identity = 27/83 (32.53%), Postives = 51/83 (61.45%), Query Frame = 0


Query: 185 PTSESAMESM-LKKVENEEIVKFGDNKSINCVICLEEISKEKKGSEAVVLQMPCLHMFHE 244
           P S+S ++++ +  + NE++++  D+ ++ C +C EE+   K+     V ++PC H +H 
Sbjct: 343 PASKSFIKNLKVSPLSNEDVME-NDDDAVCCAVCKEEMIVGKE-----VAELPCRHKYHS 402

Query: 245 ECIRKWLKRSHYCPSCRFSMPTN 267
           ECI  WL   + CP CRF +P++
Sbjct: 403 ECIVPWLGIRNTCPVCRFELPSD 419

The following BLAST results are available for this feature:

BLAST of CSPI07G06350 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8LPN7	1.5e-08	34.94	E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19...	[more]
Q940T5	1.0e-07	37.04	E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 ...	[more]
Q3T0W3	1.7e-07	28.72	E3 ubiquitin-protein ligase RNF181 OS=Bos taurus OX=9913 GN=RNF181 PE=2 SV=1	[more]
Q9P0P0	1.7e-07	27.66	E3 ubiquitin-protein ligase RNF181 OS=Homo sapiens OX=9606 GN=RNF181 PE=1 SV=1	[more]
Q9LQX2	2.9e-07	50.94	E3 ubiquitin-protein ligase MPSR1 OS=Arabidopsis thaliana OX=3702 GN=MPSR1 PE=1 ...	[more]

BLAST of CSPI07G06350 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K7L0	1.4e-150	99.63	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G067560 P...	[more]
A0A0A0K493	7.2e-102	72.76	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G067540 P...	[more]
A0A0A0K2A1	2.3e-100	74.25	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G067550 P...	[more]
A0A5D3C4K6	2.7e-96	68.63	Putative zinc finger protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca...	[more]
E5GCQ3	2.7e-96	68.63	Hypothetical zinc finger protein OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1	[more]

BLAST of CSPI07G06350 vs. NCBI nr


Match Name	E-value	Identity	Description
KAE8645828.1	5.4e-144	99.61	hypothetical protein Csa_017274 [Cucumis sativus]	[more]
KGN43799.1	1.5e-101	72.76	hypothetical protein Csa_017222 [Cucumis sativus]	[more]
ADN34252.1	5.5e-96	68.63	hypothetical zinc finger protein [Cucumis melo subsp. melo] >KAA0031412.1 putati...	[more]
ADN34253.1	1.1e-93	70.85	hypothetical zinc finger protein [Cucumis melo subsp. melo] >TYK06864.1 putative...	[more]
KAA0031413.1	2.6e-93	70.48	putative zinc finger protein [Cucumis melo var. makuwa]	[more]

BLAST of CSPI07G06350 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G19950.1	1.1e-09	34.94	RING/U-box superfamily protein	[more]
AT5G37270.1	3.2e-09	34.15	RING/U-box superfamily protein	[more]
AT5G37230.1	4.2e-09	34.15	RING/U-box superfamily protein	[more]
AT5G59550.1	7.1e-09	37.04	zinc finger (C3HC4-type RING finger) family protein	[more]
AT5G60820.1	1.6e-08	32.53	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 266..267
None	No IPR available	PANTHER	PTHR15710:SF63	RING/U-BOX SUPERFAMILY PROTEIN	coord: 150..265
None	No IPR available	PANTHER	PTHR15710	E3 UBIQUITIN-PROTEIN LIGASE PRAJA	coord: 150..265
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 197..264
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 214..259 e-value: 1.0E-7 score: 41.6
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 213..260 e-value: 2.3E-12 score: 47.1
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 214..260 score: 12.976127
IPR011016	Zinc finger, RING-CH-type	SMART	SM00744	ringv_2	coord: 213..260 e-value: 0.0093 score: 7.4
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 173..266 e-value: 9.0E-18 score: 65.7

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI07G06350.1	CSPI07G06350.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0061630	ubiquitin protein ligase activity
molecular_function	GO:0008270	zinc ion binding