CSPI07G06330 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI07G06330
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	RING-type domain-containing protein
Location	Chr7: 4676028 .. 4676825 (-)
RNA-Seq Expression	CSPI07G06330
Synteny	CSPI07G06330

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCGAACATAACTTTGATGATTGTATGTCTACTGGTTGCTTCATGGGTATGTCACTAAGAATTACCGATCCCGACTTAAGCCTTCAACCACAAAGCCAAAATCAAACTCCACACAACTCCCCAACATTTGTCAAACAAATCAACCTGAAGTTAAAAGTTGAAGTCAAATGCCAACACTTTCAAGCAGATGAAGAAGGCGACACATTTGCATCAACTTTAATGATTGAACACATAGTTTCACACCATCCCTTTCCAACTTTCCAACTTCCTGTCTCCGTCTTCAGGCATGGTAACAGAACTTTGAAGCGATTACTTTTTCAGCAGTTTCAGATATTTAGAGGTATCATCAACATTCAACTTGTGGCTGACGAAATAATAGAACACTGGGTTAAAAAAGAAGAAGATAGAGAGAATAATTCTGGGGTACTGCTTGAAGAGATATATCCTCTCGAAATTACGATAGAGTTGCTGCTTCTTCGAATGATTCATGCAATAGACCAACCGGAAGAAACGATAGATCAACCACAAGTAACGATGGTTCCAGCAAGCGAGAGTGCCATGGAGAGTATGCTGAAGAGAGTGGAAAAGGAGGAGATAGTGAAAGTGGGTGAGGACAAAAGCATTAATTGTGTTATCTGTTTGGAGGAAATTAGTAAAAAGATTAAAGGAAGTGAGGGAGTGGTGTTACAAATGCCATGCTTGCATATGTTTCATGAAGAATGTATAAGGACGTGGTTGAAGACAAGTCATTACTGCCCAACTTGTAGATTTTCAATGCCTATAAACAACAACTGA

mRNA sequence

ATGGCCGAACATAACTTTGATGATTGTATGTCTACTGGTTGCTTCATGGGTATGTCACTAAGAATTACCGATCCCGACTTAAGCCTTCAACCACAAAGCCAAAATCAAACTCCACACAACTCCCCAACATTTGTCAAACAAATCAACCTGAAGTTAAAAGTTGAAGTCAAATGCCAACACTTTCAAGCAGATGAAGAAGGCGACACATTTGCATCAACTTTAATGATTGAACACATAGTTTCACACCATCCCTTTCCAACTTTCCAACTTCCTGTCTCCGTCTTCAGGCATGGTAACAGAACTTTGAAGCGATTACTTTTTCAGCAGTTTCAGATATTTAGAGGTATCATCAACATTCAACTTGTGGCTGACGAAATAATAGAACACTGGGTTAAAAAAGAAGAAGATAGAGAGAATAATTCTGGGGTACTGCTTGAAGAGATATATCCTCTCGAAATTACGATAGAGTTGCTGCTTCTTCGAATGATTCATGCAATAGACCAACCGGAAGAAACGATAGATCAACCACAAGTAACGATGGTTCCAGCAAGCGAGAGTGCCATGGAGAGTATGCTGAAGAGAGTGGAAAAGGAGGAGATAGTGAAAGTGGGTGAGGACAAAAGCATTAATTGTGTTATCTGTTTGGAGGAAATTAGTAAAAAGATTAAAGGAAGTGAGGGAGTGGTGTTACAAATGCCATGCTTGCATATGTTTCATGAAGAATGTATAAGGACGTGGTTGAAGACAAGTCATTACTGCCCAACTTGTAGATTTTCAATGCCTATAAACAACAACTGA

Coding sequence (CDS)

ATGGCCGAACATAACTTTGATGATTGTATGTCTACTGGTTGCTTCATGGGTATGTCACTAAGAATTACCGATCCCGACTTAAGCCTTCAACCACAAAGCCAAAATCAAACTCCACACAACTCCCCAACATTTGTCAAACAAATCAACCTGAAGTTAAAAGTTGAAGTCAAATGCCAACACTTTCAAGCAGATGAAGAAGGCGACACATTTGCATCAACTTTAATGATTGAACACATAGTTTCACACCATCCCTTTCCAACTTTCCAACTTCCTGTCTCCGTCTTCAGGCATGGTAACAGAACTTTGAAGCGATTACTTTTTCAGCAGTTTCAGATATTTAGAGGTATCATCAACATTCAACTTGTGGCTGACGAAATAATAGAACACTGGGTTAAAAAAGAAGAAGATAGAGAGAATAATTCTGGGGTACTGCTTGAAGAGATATATCCTCTCGAAATTACGATAGAGTTGCTGCTTCTTCGAATGATTCATGCAATAGACCAACCGGAAGAAACGATAGATCAACCACAAGTAACGATGGTTCCAGCAAGCGAGAGTGCCATGGAGAGTATGCTGAAGAGAGTGGAAAAGGAGGAGATAGTGAAAGTGGGTGAGGACAAAAGCATTAATTGTGTTATCTGTTTGGAGGAAATTAGTAAAAAGATTAAAGGAAGTGAGGGAGTGGTGTTACAAATGCCATGCTTGCATATGTTTCATGAAGAATGTATAAGGACGTGGTTGAAGACAAGTCATTACTGCCCAACTTGTAGATTTTCAATGCCTATAAACAACAACTGA

Protein sequence

MAEHNFDDCMSTGCFMGMSLRITDPDLSLQPQSQNQTPHNSPTFVKQINLKLKVEVKCQHFQADEEGDTFASTLMIEHIVSHHPFPTFQLPVSVFRHGNRTLKRLLFQQFQIFRGIINIQLVADEIIEHWVKKEEDRENNSGVLLEEIYPLEITIELLLLRMIHAIDQPEETIDQPQVTMVPASESAMESMLKRVEKEEIVKVGEDKSINCVICLEEISKKIKGSEGVVLQMPCLHMFHEECIRTWLKTSHYCPTCRFSMPINNN*

Homology

[Close]

BLAST of CSPI07G06330 vs. ExPASy Swiss-Prot
Match: Q9SNB6 (E3 ubiquitin-protein ligase RDUF1 OS=Arabidopsis thaliana OX=3702 GN=RDUF1 PE=1 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 5.3e-09
Identity = 35/80 (43.75%), Postives = 44/80 (55.00%), Query Frame = 0


Query: 182 PASESAMESMLKRVEKEEIVKVGEDKSINCVICLEEISKKIKGSEGVVLQMPCLHMFHEE 241
           PAS+SA+ES L RVE  +     E    NC +C E     I+G E     MPC H+FH +
Sbjct: 190 PASKSAIES-LPRVEISDCHTKAE---ANCAVCTEVFEAGIEGRE-----MPCKHIFHGD 249

Query: 242 CIRTWLKTSHYCPTCRFSMP 262
           CI  WL   + CP CRF +P
Sbjct: 250 CIVPWLSIRNSCPVCRFELP 260

[Close]

BLAST of CSPI07G06330 vs. ExPASy Swiss-Prot
Match: Q940T5 (E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 6.9e-09
Identity = 35/84 (41.67%), Postives = 48/84 (57.14%), Query Frame = 0


Query: 182 PASESAMESMLKRVEKEEIVKVGEDKSINCVICLEEISKKIKGSEGVVLQMPCLHMFHEE 241
           PAS+SA+ES L RVE  +   +G +   NC +C E     I  +E    +MPC H+FH++
Sbjct: 174 PASKSAIES-LPRVEISD-CHIGSE--ANCAVCTE-----IFETETEAREMPCKHLFHDD 233

Query: 242 CIRTWLKTSHYCPTCRFSMPINNN 266
           CI  WL   + CP CRF +P   N
Sbjct: 234 CIVPWLSIRNSCPVCRFELPSEPN 248

[Close]

BLAST of CSPI07G06330 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 59.7 bits (143), Expect = 5.8e-08
Identity = 28/83 (33.73%), Postives = 51/83 (61.45%), Query Frame = 0


Query: 182 PASESAMESMLKRVEKEEIVKVGEDKSINCVICLEEISKKIKGSEGVVLQMPCLHMFHEE 241
           PAS+SA++++      ++++K    +   C +C++E      GS+  V QMPC H+FH++
Sbjct: 190 PASKSAIDALPTVKVTKDMLK---SEMNQCAVCMDEFE---DGSD--VKQMPCKHVFHQD 249

Query: 242 CIRTWLKTSHYCPTCRFSMPINN 265
           C+  WL+  + CP CRF +P ++
Sbjct: 250 CLLPWLELHNSCPVCRFELPTDD 264

[Close]

BLAST of CSPI07G06330 vs. ExPASy Swiss-Prot
Match: Q3T0W3 (E3 ubiquitin-protein ligase RNF181 OS=Bos taurus OX=9913 GN=RNF181 PE=2 SV=1)

HSP 1 Score: 58.2 bits (139), Expect = 1.7e-07
Identity = 26/84 (30.95%), Postives = 46/84 (54.76%), Query Frame = 0


Query: 182 PASESAMESMLKRVEKEEIVKVGEDKSINCVICLEEISKKIKGSEGVVLQMPCLHMFHEE 241
           PA+++A+E++ + V +      G    + C +CL E  +     E   ++MPC H+FH  
Sbjct: 53  PAAKTAVENLPRTVIR------GSQAELKCPVCLLEFEE-----EETAIEMPCHHLFHSN 112

Query: 242 CIRTWLKTSHYCPTCRFSMPINNN 266
           CI  WL  ++ CP CR  +P +++
Sbjct: 113 CILPWLSKTNSCPLCRHELPTDDD 125

[Close]

BLAST of CSPI07G06330 vs. ExPASy Swiss-Prot
Match: Q9P0P0 (E3 ubiquitin-protein ligase RNF181 OS=Homo sapiens OX=9606 GN=RNF181 PE=1 SV=1)

HSP 1 Score: 58.2 bits (139), Expect = 1.7e-07
Identity = 25/84 (29.76%), Postives = 46/84 (54.76%), Query Frame = 0


Query: 182 PASESAMESMLKRVEKEEIVKVGEDKSINCVICLEEISKKIKGSEGVVLQMPCLHMFHEE 241
           PA+++ +E++ + V +      G    + C +CL E  +     E   ++MPC H+FH  
Sbjct: 53  PAAKTVVENLPRTVIR------GSQAELKCPVCLLEFEE-----EETAIEMPCHHLFHSS 112

Query: 242 CIRTWLKTSHYCPTCRFSMPINNN 266
           CI  WL  ++ CP CR+ +P +++
Sbjct: 113 CILPWLSKTNSCPLCRYELPTDDD 125

[Close]

BLAST of CSPI07G06330 vs. ExPASy TrEMBL
Match: A0A0A0K493 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G067540 PE=4 SV=1)

HSP 1 Score: 538.9 bits (1387), Expect = 1.2e-149
Identity = 264/265 (99.62%), Postives = 265/265 (100.00%), Query Frame = 0


Query: 1   MAEHNFDDCMSTGCFMGMSLRITDPDLSLQPQSQNQTPHNSPTFVKQINLKLKVEVKCQH 60
           MAEHNFDDCMSTGCFMGMSLRITDPDLSLQPQSQNQTPHNSPTFVKQINLKLKVEVKCQH
Sbjct: 1   MAEHNFDDCMSTGCFMGMSLRITDPDLSLQPQSQNQTPHNSPTFVKQINLKLKVEVKCQH 60

Query: 61  FQADEEGDTFASTLMIEHIVSHHPFPTFQLPVSVFRHGNRTLKRLLFQQFQIFRGIINIQ 120
           FQADEEGDTFASTLMIEHIVSHHPFPTFQLPVSVFRHGNRTLKRLLFQQFQIFRGIINIQ
Sbjct: 61  FQADEEGDTFASTLMIEHIVSHHPFPTFQLPVSVFRHGNRTLKRLLFQQFQIFRGIINIQ 120

Query: 121 LVADEIIEHWVKKEEDRENNSGVLLEEIYPLEITIELLLLRMIHAIDQPEETIDQPQVTM 180
           LVADEIIEHWVKKEEDRENNSGVLLEEIYPLEITIELLLLRMIHAIDQPEETIDQPQVTM
Sbjct: 121 LVADEIIEHWVKKEEDRENNSGVLLEEIYPLEITIELLLLRMIHAIDQPEETIDQPQVTM 180

Query: 181 VPASESAMESMLKRVEKEEIVKVGEDKSINCVICLEEISKKIKGSEGVVLQMPCLHMFHE 240
           VPASESAMESMLKRVEKEEIVKVGEDKSINCVICLEEISKK+KGSEGVVLQMPCLHMFHE
Sbjct: 181 VPASESAMESMLKRVEKEEIVKVGEDKSINCVICLEEISKKMKGSEGVVLQMPCLHMFHE 240

Query: 241 ECIRTWLKTSHYCPTCRFSMPINNN 266
           ECIRTWLKTSHYCPTCRFSMPINNN
Sbjct: 241 ECIRTWLKTSHYCPTCRFSMPINNN 265

[Close]

BLAST of CSPI07G06330 vs. ExPASy TrEMBL
Match: A0A0A0K2A1 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G067550 PE=4 SV=1)

HSP 1 Score: 390.6 bits (1002), Expect = 5.3e-105
Identity = 197/266 (74.06%), Postives = 226/266 (84.96%), Query Frame = 0


Query: 1   MAEHNFDDCMSTGCFMGMSLRITDPDLSLQPQSQNQTPHNSPTFVKQINLKLKVEVKCQH 60
           MAE NFDDC S G +MGMSLRITDP          QT  NSPT VKQI+L  KVEVKCQH
Sbjct: 1   MAEQNFDDCTS-GYYMGMSLRITDP---------IQTRSNSPTSVKQIDLNFKVEVKCQH 60

Query: 61  FQADEEGDTFASTLMIEHIVSHHPFPTFQLPVSVFRHGNRTLKRLLFQQFQIFRGIINIQ 120
           FQA EE  TFA+T + EHI++H PFP  QLPVS+F+HG++TLKRLLFQ+FQ+FR IINI+
Sbjct: 61  FQAGEEDTTFATTFLSEHIIAHQPFPALQLPVSIFKHGDKTLKRLLFQKFQMFRAIINIE 120

Query: 121 LVADEIIEHWVKK-EEDRENNSGVLLEEIYPLEITIELLLLRMIHAIDQPEETIDQPQVT 180
           LV D+II+HWVKK E+D+ENNSGV  EEIYPLEITIE+L+L+MI A+DQP+ETIDQPQV 
Sbjct: 121 LVTDQIIKHWVKKVEDDKENNSGV-FEEIYPLEITIEVLVLQMIDAVDQPQETIDQPQVR 180

Query: 181 MVPASESAMESMLKRVEKEEIVKVGEDKSINCVICLEEISKKIKGSEGVVLQMPCLHMFH 240
           MVP S+SAM+SMLKRVE EEI+KVG+ KSI+CVICLE+ISK+ KGSEGVVLQMPCLHMFH
Sbjct: 181 MVPTSDSAMKSMLKRVENEEIMKVGDYKSISCVICLEKISKEEKGSEGVVLQMPCLHMFH 240

Query: 241 EECIRTWLKTSHYCPTCRFSMPINNN 266
           EECIR WLKTSHYCPTCRFSM INNN
Sbjct: 241 EECIRKWLKTSHYCPTCRFSMTINNN 255

[Close]

BLAST of CSPI07G06330 vs. ExPASy TrEMBL
Match: A0A0A0K7L0 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G067560 PE=4 SV=1)

HSP 1 Score: 381.7 bits (979), Expect = 2.5e-102
Identity = 196/268 (73.13%), Postives = 223/268 (83.21%), Query Frame = 0


Query: 1   MAEHNFDDC-MSTGCFMGMSLRITDPDLSLQPQSQNQTPHNSPTFVKQINLKLKVEVKCQ 60
           MAE  F  C   +G ++GMSLRITDPDL  QPQSQ +TP NSPT VKQI+L  KVEVKCQ
Sbjct: 1   MAEQIFGPCRYGSGSYIGMSLRITDPDLRHQPQSQIRTPPNSPTLVKQIDLNFKVEVKCQ 60

Query: 61  H-FQADEEGD-TFASTLMIEHIVSHHPFPTFQLPVSVFRHGNRTLKRLLFQQFQIFRGII 120
           H FQA E G+ TFA+ L+ EHI SHHP PT QLPVS+F+HG++TLKRLLF++FQI+R II
Sbjct: 61  HYFQAGEGGNSTFATALLSEHISSHHPLPTLQLPVSIFKHGDKTLKRLLFREFQIYRAII 120

Query: 121 NIQLVADEIIEHWVKKEEDRENNSGVLLEEIYPLEITIELLLLRMIHAIDQPEE-TIDQP 180
           NI+LV DEII+HWVKK  D EN++  + EEIYPLEITIELL+ +MIHAIDQP E  +DQP
Sbjct: 121 NIELVVDEIIKHWVKK-VDEENSTSQVFEEIYPLEITIELLVYQMIHAIDQPHEIMMDQP 180

Query: 181 QVTMVPASESAMESMLKRVEKEEIVKVGEDKSINCVICLEEISKKIKGSEGVVLQMPCLH 240
           QV MVP SESAMESMLK+VE EEIVK G++KSINCVICLEEISK+ KGSE VVLQMPCLH
Sbjct: 181 QVMMVPTSESAMESMLKKVENEEIVKFGDNKSINCVICLEEISKEKKGSEAVVLQMPCLH 240

Query: 241 MFHEECIRTWLKTSHYCPTCRFSMPINN 265
           MFHEECIR WLK SHYCPTCRFSMP NN
Sbjct: 241 MFHEECIRKWLKRSHYCPTCRFSMPTNN 267

[Close]

BLAST of CSPI07G06330 vs. ExPASy TrEMBL
Match: A0A5D3C6G9 (Putative zinc finger protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G001680 PE=4 SV=1)

HSP 1 Score: 349.4 bits (895), Expect = 1.4e-92
Identity = 184/268 (68.66%), Postives = 216/268 (80.60%), Query Frame = 0


Query: 1   MAEHNFDDCMSTGCFMGMSLRITDPDL-SLQPQSQNQTPHNSPTFVKQINLKLKVEVKCQ 60
           MAE N D CMS G +MGMSLRITDPDL   QPQSQ QT  NSP  VKQI+L  K++VKCQ
Sbjct: 1   MAEQNLDACMSAGYYMGMSLRITDPDLRHRQPQSQIQTQPNSPALVKQIDLNFKLQVKCQ 60

Query: 61  HFQADEEGDTFASTLMIEHIVSHHPFPTFQLPVSVFRHGNRTLKRLLFQQFQIFRGIINI 120
           HF A EEG TFA+TL+ EHI+S   FP  QLP+S+F+ G++TLKRLLFQ+FQ+FRGI+N+
Sbjct: 61  HFGAGEEGSTFATTLLSEHIISQPTFPALQLPISIFKRGDKTLKRLLFQKFQMFRGIVNV 120

Query: 121 QLVADEIIEHWVKK-EEDRENNSGVLLEEIYPLEITIELLLLRMIHAIDQPEETIDQPQ- 180
           +LV DEII+HW++K EED+ENNS V   EIYPLEI IELL+ RMI AI      IDQPQ 
Sbjct: 121 ELVVDEIIKHWIRKVEEDQENNSRV-FAEIYPLEIRIELLVCRMIPAI------IDQPQE 180

Query: 181 VTMVPASESAMESMLKRVE-KEEIVKVGEDKSINCVICLEEISKKIKGSEGVVLQMPCLH 240
           V MVP S++AMESMLKRVE  EE++K+G+D SI+CVICLE+I K+ K S  VVLQMPCLH
Sbjct: 181 VMMVPTSDNAMESMLKRVENNEELMKLGDD-SIDCVICLEKIGKEEKRSGRVVLQMPCLH 240

Query: 241 MFHEECIRTWLKTSHYCPTCRFSMPINN 265
           MFHEECIR WLKTSH+CPTCRFSMPINN
Sbjct: 241 MFHEECIRKWLKTSHFCPTCRFSMPINN 260

[Close]

BLAST of CSPI07G06330 vs. ExPASy TrEMBL
Match: E5GCQ4 (Hypothetical zinc finger protein OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 349.4 bits (895), Expect = 1.4e-92
Identity = 184/268 (68.66%), Postives = 216/268 (80.60%), Query Frame = 0


Query: 1   MAEHNFDDCMSTGCFMGMSLRITDPDL-SLQPQSQNQTPHNSPTFVKQINLKLKVEVKCQ 60
           MAE N D CMS G +MGMSLRITDPDL   QPQSQ QT  NSP  VKQI+L  K++VKCQ
Sbjct: 1   MAEQNLDACMSAGYYMGMSLRITDPDLRHRQPQSQIQTQPNSPALVKQIDLNFKLQVKCQ 60

Query: 61  HFQADEEGDTFASTLMIEHIVSHHPFPTFQLPVSVFRHGNRTLKRLLFQQFQIFRGIINI 120
           HF A EEG TFA+TL+ EHI+S   FP  QLP+S+F+ G++TLKRLLFQ+FQ+FRGI+N+
Sbjct: 61  HFGAGEEGSTFATTLLSEHIISQPTFPALQLPISIFKRGDKTLKRLLFQKFQMFRGIVNV 120

Query: 121 QLVADEIIEHWVKK-EEDRENNSGVLLEEIYPLEITIELLLLRMIHAIDQPEETIDQPQ- 180
           +LV DEII+HW++K EED+ENNS V   EIYPLEI IELL+ RMI AI      IDQPQ 
Sbjct: 121 ELVVDEIIKHWIRKVEEDQENNSRV-FAEIYPLEIRIELLVCRMIPAI------IDQPQE 180

Query: 181 VTMVPASESAMESMLKRVE-KEEIVKVGEDKSINCVICLEEISKKIKGSEGVVLQMPCLH 240
           V MVP S++AMESMLKRVE  EE++K+G+D SI+CVICLE+I K+ K S  VVLQMPCLH
Sbjct: 181 VMMVPTSDNAMESMLKRVENNEELMKLGDD-SIDCVICLEKIGKEEKRSGRVVLQMPCLH 240

Query: 241 MFHEECIRTWLKTSHYCPTCRFSMPINN 265
           MFHEECIR WLKTSH+CPTCRFSMPINN
Sbjct: 241 MFHEECIRKWLKTSHFCPTCRFSMPINN 260

[Close]

BLAST of CSPI07G06330 vs. NCBI nr
Match: KGN43799.1 (hypothetical protein Csa_017222 [Cucumis sativus])

HSP 1 Score: 538.9 bits (1387), Expect = 2.5e-149
Identity = 264/265 (99.62%), Postives = 265/265 (100.00%), Query Frame = 0


Query: 1   MAEHNFDDCMSTGCFMGMSLRITDPDLSLQPQSQNQTPHNSPTFVKQINLKLKVEVKCQH 60
           MAEHNFDDCMSTGCFMGMSLRITDPDLSLQPQSQNQTPHNSPTFVKQINLKLKVEVKCQH
Sbjct: 1   MAEHNFDDCMSTGCFMGMSLRITDPDLSLQPQSQNQTPHNSPTFVKQINLKLKVEVKCQH 60

Query: 61  FQADEEGDTFASTLMIEHIVSHHPFPTFQLPVSVFRHGNRTLKRLLFQQFQIFRGIINIQ 120
           FQADEEGDTFASTLMIEHIVSHHPFPTFQLPVSVFRHGNRTLKRLLFQQFQIFRGIINIQ
Sbjct: 61  FQADEEGDTFASTLMIEHIVSHHPFPTFQLPVSVFRHGNRTLKRLLFQQFQIFRGIINIQ 120

Query: 121 LVADEIIEHWVKKEEDRENNSGVLLEEIYPLEITIELLLLRMIHAIDQPEETIDQPQVTM 180
           LVADEIIEHWVKKEEDRENNSGVLLEEIYPLEITIELLLLRMIHAIDQPEETIDQPQVTM
Sbjct: 121 LVADEIIEHWVKKEEDRENNSGVLLEEIYPLEITIELLLLRMIHAIDQPEETIDQPQVTM 180

Query: 181 VPASESAMESMLKRVEKEEIVKVGEDKSINCVICLEEISKKIKGSEGVVLQMPCLHMFHE 240
           VPASESAMESMLKRVEKEEIVKVGEDKSINCVICLEEISKK+KGSEGVVLQMPCLHMFHE
Sbjct: 181 VPASESAMESMLKRVEKEEIVKVGEDKSINCVICLEEISKKMKGSEGVVLQMPCLHMFHE 240

Query: 241 ECIRTWLKTSHYCPTCRFSMPINNN 266
           ECIRTWLKTSHYCPTCRFSMPINNN
Sbjct: 241 ECIRTWLKTSHYCPTCRFSMPINNN 265

[Close]

BLAST of CSPI07G06330 vs. NCBI nr
Match: KAE8645828.1 (hypothetical protein Csa_017274 [Cucumis sativus])

HSP 1 Score: 393.7 bits (1010), Expect = 1.3e-105
Identity = 198/266 (74.44%), Postives = 227/266 (85.34%), Query Frame = 0


Query: 1   MAEHNFDDCMSTGCFMGMSLRITDPDLSLQPQSQNQTPHNSPTFVKQINLKLKVEVKCQH 60
           MAE NFDDC S G +MGMSLRITDP          QT  NSPT VKQI+L  KVEVKCQH
Sbjct: 267 MAEQNFDDCTS-GYYMGMSLRITDP---------IQTRSNSPTSVKQIDLNFKVEVKCQH 326

Query: 61  FQADEEGDTFASTLMIEHIVSHHPFPTFQLPVSVFRHGNRTLKRLLFQQFQIFRGIINIQ 120
           FQA EE  TFA+T + EHI++H PFP  QLPVS+F+HG++TLKRLLFQ+FQ+FR IINI+
Sbjct: 327 FQAGEEDTTFATTFLSEHIIAHQPFPALQLPVSIFKHGDKTLKRLLFQKFQMFRAIINIE 386

Query: 121 LVADEIIEHWVKK-EEDRENNSGVLLEEIYPLEITIELLLLRMIHAIDQPEETIDQPQVT 180
           LV D+II+HWVKK E+D+ENNSGV  EEIYPLEITIE+L+L+MI A+DQP+ETIDQPQV 
Sbjct: 387 LVTDQIIKHWVKKVEDDKENNSGV-FEEIYPLEITIEVLVLQMIDAVDQPQETIDQPQVR 446

Query: 181 MVPASESAMESMLKRVEKEEIVKVGEDKSINCVICLEEISKKIKGSEGVVLQMPCLHMFH 240
           MVP S+SAM+SMLKRVE EEI+KVG+ KSI+CVICLE+ISK+ KGSEGVVLQMPCLHMFH
Sbjct: 447 MVPTSDSAMKSMLKRVENEEIMKVGDYKSISCVICLEKISKEEKGSEGVVLQMPCLHMFH 506

Query: 241 EECIRTWLKTSHYCPTCRFSMPINNN 266
           EECIR WLKTSHYCPTCRFSMPINNN
Sbjct: 507 EECIRKWLKTSHYCPTCRFSMPINNN 521

[Close]

BLAST of CSPI07G06330 vs. NCBI nr
Match: ADN34253.1 (hypothetical zinc finger protein [Cucumis melo subsp. melo] >TYK06864.1 putative zinc finger protein [Cucumis melo var. makuwa])

HSP 1 Score: 349.4 bits (895), Expect = 2.8e-92
Identity = 184/268 (68.66%), Postives = 216/268 (80.60%), Query Frame = 0


Query: 1   MAEHNFDDCMSTGCFMGMSLRITDPDL-SLQPQSQNQTPHNSPTFVKQINLKLKVEVKCQ 60
           MAE N D CMS G +MGMSLRITDPDL   QPQSQ QT  NSP  VKQI+L  K++VKCQ
Sbjct: 1   MAEQNLDACMSAGYYMGMSLRITDPDLRHRQPQSQIQTQPNSPALVKQIDLNFKLQVKCQ 60

Query: 61  HFQADEEGDTFASTLMIEHIVSHHPFPTFQLPVSVFRHGNRTLKRLLFQQFQIFRGIINI 120
           HF A EEG TFA+TL+ EHI+S   FP  QLP+S+F+ G++TLKRLLFQ+FQ+FRGI+N+
Sbjct: 61  HFGAGEEGSTFATTLLSEHIISQPTFPALQLPISIFKRGDKTLKRLLFQKFQMFRGIVNV 120

Query: 121 QLVADEIIEHWVKK-EEDRENNSGVLLEEIYPLEITIELLLLRMIHAIDQPEETIDQPQ- 180
           +LV DEII+HW++K EED+ENNS V   EIYPLEI IELL+ RMI AI      IDQPQ 
Sbjct: 121 ELVVDEIIKHWIRKVEEDQENNSRV-FAEIYPLEIRIELLVCRMIPAI------IDQPQE 180

Query: 181 VTMVPASESAMESMLKRVE-KEEIVKVGEDKSINCVICLEEISKKIKGSEGVVLQMPCLH 240
           V MVP S++AMESMLKRVE  EE++K+G+D SI+CVICLE+I K+ K S  VVLQMPCLH
Sbjct: 181 VMMVPTSDNAMESMLKRVENNEELMKLGDD-SIDCVICLEKIGKEEKRSGRVVLQMPCLH 240

Query: 241 MFHEECIRTWLKTSHYCPTCRFSMPINN 265
           MFHEECIR WLKTSH+CPTCRFSMPINN
Sbjct: 241 MFHEECIRKWLKTSHFCPTCRFSMPINN 260

[Close]

BLAST of CSPI07G06330 vs. NCBI nr
Match: KAA0031413.1 (putative zinc finger protein [Cucumis melo var. makuwa])

HSP 1 Score: 346.3 bits (887), Expect = 2.4e-91
Identity = 178/267 (66.67%), Postives = 213/267 (79.78%), Query Frame = 0


Query: 1   MAEHNFDDCMSTGCFMGMSLRITDPDL-SLQPQSQNQTPHNSPTFVKQINLKLKVEVKCQ 60
           MAE N D CMS G +MGMSLRITDPDL   QPQSQ QT  NSP  VKQI+L  K++VKCQ
Sbjct: 1   MAEQNLDACMSAGYYMGMSLRITDPDLRHRQPQSQIQTQPNSPALVKQIDLNFKLQVKCQ 60

Query: 61  HFQADEEGDTFASTLMIEHIVSHHPFPTFQLPVSVFRHGNRTLKRLLFQQFQIFRGIINI 120
           HF A EEG TFA+TL+ EHI+S   FP  QLP+S+F+ G++TLKRLLFQ+FQ+FRGI+N+
Sbjct: 61  HFGAGEEGSTFATTLLSEHIISQPTFPALQLPISIFKRGDKTLKRLLFQKFQMFRGIVNV 120

Query: 121 QLVADEIIEHWVKKEEDRENNSGVLLEEIYPLEITIELLLLRMIHAIDQPEETIDQPQ-V 180
           +LV DEII+HW++K E+ + NS  +  EIYPLEI IELL+ RMI AI      IDQPQ V
Sbjct: 121 ELVVDEIIKHWIRKVEEDQENSSRVFAEIYPLEIRIELLVCRMIPAI------IDQPQEV 180

Query: 181 TMVPASESAMESMLKRVE-KEEIVKVGEDKSINCVICLEEISKKIKGSEGVVLQMPCLHM 240
            MVP S++AMESMLKRVE  +E++K+G+D SI+CVICLE+I K+ K S  VVLQMPCLHM
Sbjct: 181 MMVPTSDNAMESMLKRVENNKELMKLGDD-SIDCVICLEKIGKEEKRSGRVVLQMPCLHM 240

Query: 241 FHEECIRTWLKTSHYCPTCRFSMPINN 265
           FHEECIR WLKTSH+CPTCRFSMPINN
Sbjct: 241 FHEECIRKWLKTSHFCPTCRFSMPINN 260

[Close]

BLAST of CSPI07G06330 vs. NCBI nr
Match: ADN34252.1 (hypothetical zinc finger protein [Cucumis melo subsp. melo] >KAA0031412.1 putative zinc finger protein [Cucumis melo var. makuwa] >TYK06863.1 putative zinc finger protein [Cucumis melo var. makuwa])

HSP 1 Score: 330.5 bits (846), Expect = 1.3e-86
Identity = 171/269 (63.57%), Postives = 215/269 (79.93%), Query Frame = 0


Query: 1   MAEHNFDDC-MSTGCFMGMSLRITDPDLSLQPQSQ-NQTPHNSPTFVKQINLKLKVEVKC 60
           MAE  F+ C   +G +MGMSLRITDP+   QP +Q +QT  +SPT V+QI LK KV++KC
Sbjct: 1   MAEEIFNICRYGSGSYMGMSLRITDPNPRHQPLNQIDQT--SSPTLVEQICLKFKVQLKC 60

Query: 61  QHFQADEEGDTFASTLMIEHIVSHHPFPTFQLPVSVFRHGNRTLKRLLFQQFQIFRGIIN 120
           QHFQ  E G+  ++TL+ EHI S H F TFQLP+S+F+HG++TLKR+LF++F+++  IIN
Sbjct: 61  QHFQVWEGGNGHSTTLVREHIRSQHSFATFQLPISIFKHGDKTLKRILFREFKMYGDIIN 120

Query: 121 IQLVADEIIEHWVKK---EEDRENNSGVLLEEIYPLEITIELLLLRMIHAIDQPEETIDQ 180
           I+L+A+EII+HWV+K   EED+ENNS VL ++IYPLEI I LL+ +MI A+      IDQ
Sbjct: 121 IELLANEIIKHWVRKVEEEEDQENNSNVLFKKIYPLEIMIRLLVSKMIRAVQPRLAMIDQ 180

Query: 181 PQVTMVPASESAMESMLKRVEKEEIVKVGEDKSINCVICLEEISKKIKGSEGVVLQMPCL 240
           P   MVP S+SA+ESMLKRVE EEI+K G+D+SINCV+CLEEISK+ KGSE  VLQMPCL
Sbjct: 181 P--LMVPTSDSAVESMLKRVENEEIMKSGDDESINCVVCLEEISKEEKGSETTVLQMPCL 240

Query: 241 HMFHEECIRTWLKTSHYCPTCRFSMPINN 265
           HMFHEECIR WLKTSHYCPTCRFSMPI+N
Sbjct: 241 HMFHEECIRKWLKTSHYCPTCRFSMPIDN 265

[Close]

BLAST of CSPI07G06330 vs. TAIR 10
Match: AT3G46620.1 (zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 63.2 bits (152), Expect = 3.7e-10
Identity = 35/80 (43.75%), Postives = 44/80 (55.00%), Query Frame = 0


Query: 182 PASESAMESMLKRVEKEEIVKVGEDKSINCVICLEEISKKIKGSEGVVLQMPCLHMFHEE 241
           PAS+SA+ES L RVE  +     E    NC +C E     I+G E     MPC H+FH +
Sbjct: 190 PASKSAIES-LPRVEISDCHTKAE---ANCAVCTEVFEAGIEGRE-----MPCKHIFHGD 249

Query: 242 CIRTWLKTSHYCPTCRFSMP 262
           CI  WL   + CP CRF +P
Sbjct: 250 CIVPWLSIRNSCPVCRFELP 260

[Close]

BLAST of CSPI07G06330 vs. TAIR 10
Match: AT5G59550.1 (zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 62.8 bits (151), Expect = 4.9e-10
Identity = 35/84 (41.67%), Postives = 48/84 (57.14%), Query Frame = 0


Query: 182 PASESAMESMLKRVEKEEIVKVGEDKSINCVICLEEISKKIKGSEGVVLQMPCLHMFHEE 241
           PAS+SA+ES L RVE  +   +G +   NC +C E     I  +E    +MPC H+FH++
Sbjct: 174 PASKSAIES-LPRVEISD-CHIGSE--ANCAVCTE-----IFETETEAREMPCKHLFHDD 233

Query: 242 CIRTWLKTSHYCPTCRFSMPINNN 266
           CI  WL   + CP CRF +P   N
Sbjct: 234 CIVPWLSIRNSCPVCRFELPSEPN 248

[Close]

BLAST of CSPI07G06330 vs. TAIR 10
Match: AT3G58720.1 (RING/U-box superfamily protein )

HSP 1 Score: 60.8 bits (146), Expect = 1.9e-09
Identity = 27/55 (49.09%), Postives = 31/55 (56.36%), Query Frame = 0


Query: 204 GEDKSINCVICLEEISKKIKGSEGVVLQMPCLHMFHEECIRTWLKTSHYCPTCRF 259
           GE+    C +CLE+   K       V+  PC HMFHEECI  WLKT   CP CRF
Sbjct: 137 GEEDDKRCSVCLEDFEPK-----ETVMLTPCKHMFHEECIVPWLKTKGQCPVCRF 186

[Close]

BLAST of CSPI07G06330 vs. TAIR 10
Match: AT3G58720.2 (RING/U-box superfamily protein )

HSP 1 Score: 60.8 bits (146), Expect = 1.9e-09
Identity = 27/55 (49.09%), Postives = 31/55 (56.36%), Query Frame = 0


Query: 204 GEDKSINCVICLEEISKKIKGSEGVVLQMPCLHMFHEECIRTWLKTSHYCPTCRF 259
           GE+    C +CLE+   K       V+  PC HMFHEECI  WLKT   CP CRF
Sbjct: 165 GEEDDKRCSVCLEDFEPK-----ETVMLTPCKHMFHEECIVPWLKTKGQCPVCRF 214

[Close]

BLAST of CSPI07G06330 vs. TAIR 10
Match: AT3G13228.1 (RING/U-box superfamily protein )

HSP 1 Score: 60.5 bits (145), Expect = 2.4e-09
Identity = 28/80 (35.00%), Postives = 49/80 (61.25%), Query Frame = 0


Query: 182 PASESAMESMLKRVEKEEIVKVGEDKSINCVICLEEISKKIKGSEGVVLQMPCLHMFHEE 241
           PAS+  ++S+ +++  ++I   GE     C ICLEE +     + G+++ +PC H F +E
Sbjct: 251 PASKLVVKSLTRKI-YDKINYTGE----RCTICLEEFN-----AGGILVALPCGHDFDDE 310

Query: 242 CIRTWLKTSHYCPTCRFSMP 262
           C   W +T+H+CP CR+ +P
Sbjct: 311 CAVKWFETNHFCPLCRYELP 320

The following BLAST results are available for this feature:

BLAST of CSPI07G06330 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SNB6	5.3e-09	43.75	E3 ubiquitin-protein ligase RDUF1 OS=Arabidopsis thaliana OX=3702 GN=RDUF1 PE=1 ...	[more]
Q940T5	6.9e-09	41.67	E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 ...	[more]
Q8LPN7	5.8e-08	33.73	E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19...	[more]
Q3T0W3	1.7e-07	30.95	E3 ubiquitin-protein ligase RNF181 OS=Bos taurus OX=9913 GN=RNF181 PE=2 SV=1	[more]
Q9P0P0	1.7e-07	29.76	E3 ubiquitin-protein ligase RNF181 OS=Homo sapiens OX=9606 GN=RNF181 PE=1 SV=1	[more]

BLAST of CSPI07G06330 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K493	1.2e-149	99.62	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G067540 P...	[more]
A0A0A0K2A1	5.3e-105	74.06	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G067550 P...	[more]
A0A0A0K7L0	2.5e-102	73.13	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G067560 P...	[more]
A0A5D3C6G9	1.4e-92	68.66	Putative zinc finger protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca...	[more]
E5GCQ4	1.4e-92	68.66	Hypothetical zinc finger protein OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1	[more]

BLAST of CSPI07G06330 vs. NCBI nr


Match Name	E-value	Identity	Description
KGN43799.1	2.5e-149	99.62	hypothetical protein Csa_017222 [Cucumis sativus]	[more]
KAE8645828.1	1.3e-105	74.44	hypothetical protein Csa_017274 [Cucumis sativus]	[more]
ADN34253.1	2.8e-92	68.66	hypothetical zinc finger protein [Cucumis melo subsp. melo] >TYK06864.1 putative...	[more]
KAA0031413.1	2.4e-91	66.67	putative zinc finger protein [Cucumis melo var. makuwa]	[more]
ADN34252.1	1.3e-86	63.57	hypothetical zinc finger protein [Cucumis melo subsp. melo] >KAA0031412.1 putati...	[more]

BLAST of CSPI07G06330 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G46620.1	3.7e-10	43.75	zinc finger (C3HC4-type RING finger) family protein	[more]
AT5G59550.1	4.9e-10	41.67	zinc finger (C3HC4-type RING finger) family protein	[more]
AT3G58720.1	1.9e-09	49.09	RING/U-box superfamily protein	[more]
AT3G58720.2	1.9e-09	49.09	RING/U-box superfamily protein	[more]
AT3G13228.1	2.4e-09	35.00	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR011016	Zinc finger, RING-CH-type	SMART	SM00744	ringv_2	coord: 210..257 e-value: 9.0E-4 score: 16.2
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 211..256 e-value: 7.3E-8 score: 42.1
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 210..257 e-value: 5.8E-12 score: 45.8
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 211..257 score: 12.902362
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 170..263 e-value: 4.3E-18 score: 66.8
None	No IPR available	PANTHER	PTHR15710:SF77	OS07G0631200 PROTEIN	coord: 114..259
None	No IPR available	PANTHER	PTHR15710	E3 UBIQUITIN-PROTEIN LIGASE PRAJA	coord: 114..259
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 198..262

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI07G06330.1	CSPI07G06330.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0061630	ubiquitin protein ligase activity
molecular_function	GO:0008270	zinc ion binding