CSPI07G06200 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI07G06200
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionPhloem filament protein
LocationChr7: 4593631 .. 4594339 (+)
RNA-Seq ExpressionCSPI07G06200
SyntenyCSPI07G06200
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATATTCTTTCCAAAATTATTCTTTGACTTGCACTATTTCTCCCATGAATACACTTGTATATAAAGCTTCAACAACTCATGCAATGGCAACCCACCAAACAAAATTTTCAATACTATCAAATATCAACTAAATTAGCTCACATAAAAATGACGATTGGTATTGGTGGAGGAATTGGTATTGGCATTGGCGTTCCTGGTATCAAAGTATCGATCGGTGGAGGCATCGGTATCGGAAGTAGAAAGGGGGGGTGCAAGAGTCATGATCCTTCAAAAAAATGGAGTCAAGTTCCCAATGCGCATGCAGATCAAGTTATTATAAATGTTGCTAACTTTGCAGTGGCAGCGTTCAACCAAAAATATGGAAATAGGCTTGTGTTTCATGCAGTTTTGGAAGCTTGGGTTGTTGTTCTTCCTGATGGCAAAAAAGAGTATAGTATTGAATTAGTAGCAAAAGACTGTTTGCATCGTTGCCTCTACTTTCATGCTATTGTGATTGAAATAGTTGGCGTTCCCCCTGGCTGGAATCTTTTTTCTTTCACCCAAATCATCGACTAAATTAAAAGAAAAGTTATATGTATAATCGAAGTTCATACTTATGTGTGAGAAGTACACAATAATAAATCTTATATGATACTGTACTGCTCTACTTTTTTTAACTATCTGTTATAGAATAATAATGGAGGCCAAACGACGTTTTTAGTCCCAAACCC

mRNA sequence

ATATTCTTTCCAAAATTATTCTTTGACTTGCACTATTTCTCCCATGAATACACTTGTATATAAAGCTTCAACAACTCATGCAATGGCAACCCACCAAACAAAATTTTCAATACTATCAAATATCAACTAAATTAGCTCACATAAAAATGACGATTGGTATTGGTGGAGGAATTGGTATTGGCATTGGCGTTCCTGGTATCAAAGTATCGATCGGTGGAGGCATCGGTATCGGAAGTAGAAAGGGGGGGTGCAAGAGTCATGATCCTTCAAAAAAATGGAGTCAAGTTCCCAATGCGCATGCAGATCAAGTTATTATAAATGTTGCTAACTTTGCAGTGGCAGCGTTCAACCAAAAATATGGAAATAGGCTTGTGTTTCATGCAGTTTTGGAAGCTTGGGTTGTTGTTCTTCCTGATGGCAAAAAAGAGTATAGTATTGAATTAGTAGCAAAAGACTGTTTGCATCGTTGCCTCTACTTTCATGCTATTGTGATTGAAATAGTTGGCGTTCCCCCTGGCTGGAATCTTTTTTCTTTCACCCAAATCATCGACTAAATTAAAAGAAAAGTTATATGTATAATCGAAGTTCATACTTATGTGTGAGAAGTACACAATAATAAATCTTATATGATACTGTACTGCTCTACTTTTTTTAACTATCTGTTATAGAATAATAATGGAGGCCAAACGACGTTTTTAGTCCCAAACCC

Coding sequence (CDS)

ATGCAATGGCAACCCACCAAACAAAATTTTCAATACTATCAAATATCAACTAAATTAGCTCACATAAAAATGACGATTGGTATTGGTGGAGGAATTGGTATTGGCATTGGCGTTCCTGGTATCAAAGTATCGATCGGTGGAGGCATCGGTATCGGAAGTAGAAAGGGGGGGTGCAAGAGTCATGATCCTTCAAAAAAATGGAGTCAAGTTCCCAATGCGCATGCAGATCAAGTTATTATAAATGTTGCTAACTTTGCAGTGGCAGCGTTCAACCAAAAATATGGAAATAGGCTTGTGTTTCATGCAGTTTTGGAAGCTTGGGTTGTTGTTCTTCCTGATGGCAAAAAAGAGTATAGTATTGAATTAGTAGCAAAAGACTGTTTGCATCGTTGCCTCTACTTTCATGCTATTGTGATTGAAATAGTTGGCGTTCCCCCTGGCTGGAATCTTTTTTCTTTCACCCAAATCATCGACTAA

Protein sequence

MQWQPTKQNFQYYQISTKLAHIKMTIGIGGGIGIGIGVPGIKVSIGGGIGIGSRKGGCKSHDPSKKWSQVPNAHADQVIINVANFAVAAFNQKYGNRLVFHAVLEAWVVVLPDGKKEYSIELVAKDCLHRCLYFHAIVIEIVGVPPGWNLFSFTQIID*
Homology
BLAST of CSPI07G06200 vs. ExPASy TrEMBL
Match: A0A0A0K477 (PP1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G067390 PE=4 SV=1)

HSP 1 Score: 316.6 bits (810), Expect = 5.8e-83
Identity = 154/158 (97.47%), Postives = 156/158 (98.73%), Query Frame = 0

Query: 1   MQWQPTKQNFQYYQISTKLAHIKMTIGIGGGIGIGIGVPGIKVSIGGGIGIGSRKGGCKS 60
           MQWQPTKQNFQYYQISTKLAHIKMTIGIGGGIGIGIGVPGIKVSIGGGIGIGSRKGGCKS
Sbjct: 1   MQWQPTKQNFQYYQISTKLAHIKMTIGIGGGIGIGIGVPGIKVSIGGGIGIGSRKGGCKS 60

Query: 61  HDPSKKWSQVPNAHADQVIINVANFAVAAFNQKYGNRLVFHAVLEAWVVVLPDGKKEYSI 120
           HDPSKKWSQVPNA ADQVIINVANFAVAAFNQKYGN+LVFHAVLEAWVVVLPDGKKEYSI
Sbjct: 61  HDPSKKWSQVPNAQADQVIINVANFAVAAFNQKYGNKLVFHAVLEAWVVVLPDGKKEYSI 120

Query: 121 ELVAKDCLHRCLYFHAIVIEIVGVPPGWNLFSFTQIID 159
           ELVAKDCL+RCLYFHAIVIEIVGVPPGWNLFSFTQI D
Sbjct: 121 ELVAKDCLNRCLYFHAIVIEIVGVPPGWNLFSFTQISD 158

BLAST of CSPI07G06200 vs. ExPASy TrEMBL
Match: A0A5D3C621 (Phloem filament protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G001790 PE=4 SV=1)

HSP 1 Score: 207.6 bits (527), Expect = 3.8e-50
Identity = 98/135 (72.59%), Postives = 113/135 (83.70%), Query Frame = 0

Query: 24  MTIGIGGGIGIGIGVPGIKVSIGGGIGIGSRKGGCKSHDPSKKWSQVPNAHADQVIINVA 83
           MT+GIGGGIGIG+G+PGI +SIGGGIGI  RKGGCKSHDPSKKW+++ + + D  II +A
Sbjct: 1   MTLGIGGGIGIGLGIPGINISIGGGIGIRGRKGGCKSHDPSKKWTEIKSVN-DPAIIEIA 60

Query: 84  NFAVAAFNQKYGNRLVFHAVLEAWVVVLPDGKKEYSIELVAKDCLHRCLYFHAIVIEIVG 143
            FAV  FNQK+GN L F AVLEAWVVV  DGKKEYSIELVAKDCLHRCL +HA+VIEI G
Sbjct: 61  KFAVKVFNQKHGNNLQFQAVLEAWVVVHVDGKKEYSIELVAKDCLHRCLQYHAVVIEICG 120

Query: 144 VPPGWNLFSFTQIID 159
            PPGWNL SF QI++
Sbjct: 121 TPPGWNLVSFNQIVE 134

BLAST of CSPI07G06200 vs. ExPASy TrEMBL
Match: A0A5A7SJV3 (Phloem filament protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold139G001800 PE=4 SV=1)

HSP 1 Score: 205.3 bits (521), Expect = 1.9e-49
Identity = 97/135 (71.85%), Postives = 112/135 (82.96%), Query Frame = 0

Query: 24  MTIGIGGGIGIGIGVPGIKVSIGGGIGIGSRKGGCKSHDPSKKWSQVPNAHADQVIINVA 83
           MT+GIGGGI IG+G+PGI +SIGGGIGI  RKGGCKSHDPSKKW+++ + + D  II +A
Sbjct: 1   MTLGIGGGISIGLGIPGINISIGGGIGIRGRKGGCKSHDPSKKWTEIKSVN-DPAIIEIA 60

Query: 84  NFAVAAFNQKYGNRLVFHAVLEAWVVVLPDGKKEYSIELVAKDCLHRCLYFHAIVIEIVG 143
            FAV  FNQK+GN L F AVLEAWVVV  DGKKEYSIELVAKDCLHRCL +HA+VIEI G
Sbjct: 61  KFAVKVFNQKHGNNLQFQAVLEAWVVVHVDGKKEYSIELVAKDCLHRCLQYHAVVIEICG 120

Query: 144 VPPGWNLFSFTQIID 159
            PPGWNL SF QI++
Sbjct: 121 TPPGWNLVSFNQIVE 134

BLAST of CSPI07G06200 vs. ExPASy TrEMBL
Match: E5GCR5 (Phloem filament protein OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 205.3 bits (521), Expect = 1.9e-49
Identity = 97/135 (71.85%), Postives = 112/135 (82.96%), Query Frame = 0

Query: 24  MTIGIGGGIGIGIGVPGIKVSIGGGIGIGSRKGGCKSHDPSKKWSQVPNAHADQVIINVA 83
           MT+GIGGGI IG+G+PGI +SIGGGIGI  RKGGCKSHDPSKKW+++ + + D  II +A
Sbjct: 1   MTLGIGGGISIGLGIPGINISIGGGIGIRGRKGGCKSHDPSKKWTEIKSVN-DPAIIEIA 60

Query: 84  NFAVAAFNQKYGNRLVFHAVLEAWVVVLPDGKKEYSIELVAKDCLHRCLYFHAIVIEIVG 143
            FAV  FNQK+GN L F AVLEAWVVV  DGKKEYSIELVAKDCLHRCL +HA+VIEI G
Sbjct: 61  KFAVKVFNQKHGNNLQFQAVLEAWVVVHVDGKKEYSIELVAKDCLHRCLQYHAVVIEICG 120

Query: 144 VPPGWNLFSFTQIID 159
            PPGWNL SF QI++
Sbjct: 121 TPPGWNLVSFNQIVE 134

BLAST of CSPI07G06200 vs. ExPASy TrEMBL
Match: A0A5A7SQL4 (Phloem filament protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold139G001810 PE=4 SV=1)

HSP 1 Score: 152.5 bits (384), Expect = 1.5e-33
Identity = 84/136 (61.76%), Postives = 96/136 (70.59%), Query Frame = 0

Query: 24  MTIGIGGGIGIGIGVPGIKVSIGGGIGIGSRKGGCKSHDPSKKWSQVPNAHADQVIINVA 83
           MT+GIGG IGIG G+PG  +SIGGGIGIG RKGGCKSH+PSKKW Q+ N  A  V +  A
Sbjct: 1   MTLGIGGRIGIGFGLPGFNISIGGGIGIGGRKGGCKSHNPSKKWIQLHNVKAPDV-LEAA 60

Query: 84  NFAVAAFNQKYGNRLVFHAVLEAWVVVLPDGKKEYSIELVAKDCLHRCLYFHAIVIE-IV 143
            FAV AFN+KY N L F  V EAWV  + + +KEYSIELVAKDC HR L FHA+VIE  V
Sbjct: 61  KFAVDAFNKKYENNLEFQYVTEAWVHEV-NNEKEYSIELVAKDCFHRLLEFHAVVIEKTV 120

Query: 144 GVPPGWNLFSFTQIID 159
                W L SF +I D
Sbjct: 121 DHKKIWILVSFERIFD 134

BLAST of CSPI07G06200 vs. NCBI nr
Match: KAE8645826.1 (hypothetical protein Csa_017081 [Cucumis sativus])

HSP 1 Score: 276.2 bits (705), Expect = 1.8e-70
Identity = 135/139 (97.12%), Postives = 137/139 (98.56%), Query Frame = 0

Query: 20  AHIKMTIGIGGGIGIGIGVPGIKVSIGGGIGIGSRKGGCKSHDPSKKWSQVPNAHADQVI 79
           AHIKMTIGIGGGIGIGIGVPGIKVSIGGGIGIGSRKGGCKSHDPSKKWSQVPNA ADQVI
Sbjct: 5   AHIKMTIGIGGGIGIGIGVPGIKVSIGGGIGIGSRKGGCKSHDPSKKWSQVPNAQADQVI 64

Query: 80  INVANFAVAAFNQKYGNRLVFHAVLEAWVVVLPDGKKEYSIELVAKDCLHRCLYFHAIVI 139
           INVANFAVAAFNQKYGN+LVFHAVLEAWVVVLPDGKKEYSIELVAKDCL+RCLYFHAIVI
Sbjct: 65  INVANFAVAAFNQKYGNKLVFHAVLEAWVVVLPDGKKEYSIELVAKDCLNRCLYFHAIVI 124

Query: 140 EIVGVPPGWNLFSFTQIID 159
           EIVGVPPGWNLFSFTQI D
Sbjct: 125 EIVGVPPGWNLFSFTQISD 143

BLAST of CSPI07G06200 vs. NCBI nr
Match: XP_011658840.2 (uncharacterized protein LOC105436098 [Cucumis sativus])

HSP 1 Score: 267.3 bits (682), Expect = 8.4e-68
Identity = 131/135 (97.04%), Postives = 133/135 (98.52%), Query Frame = 0

Query: 24  MTIGIGGGIGIGIGVPGIKVSIGGGIGIGSRKGGCKSHDPSKKWSQVPNAHADQVIINVA 83
           MTIGIGGGIGIGIGVPGIKVSIGGGIGIGSRKGGCKSHDPSKKWSQVPNA ADQVIINVA
Sbjct: 1   MTIGIGGGIGIGIGVPGIKVSIGGGIGIGSRKGGCKSHDPSKKWSQVPNAQADQVIINVA 60

Query: 84  NFAVAAFNQKYGNRLVFHAVLEAWVVVLPDGKKEYSIELVAKDCLHRCLYFHAIVIEIVG 143
           NFAVAAFNQKYGN+LVFHAVLEAWVVVLPDGKKEYSIELVAKDCL+RCLYFHAIVIEIVG
Sbjct: 61  NFAVAAFNQKYGNKLVFHAVLEAWVVVLPDGKKEYSIELVAKDCLNRCLYFHAIVIEIVG 120

Query: 144 VPPGWNLFSFTQIID 159
           VPPGWNLFSFTQI D
Sbjct: 121 VPPGWNLFSFTQISD 135

BLAST of CSPI07G06200 vs. NCBI nr
Match: TYK06875.1 (phloem filament protein [Cucumis melo var. makuwa])

HSP 1 Score: 207.6 bits (527), Expect = 7.9e-50
Identity = 98/135 (72.59%), Postives = 113/135 (83.70%), Query Frame = 0

Query: 24  MTIGIGGGIGIGIGVPGIKVSIGGGIGIGSRKGGCKSHDPSKKWSQVPNAHADQVIINVA 83
           MT+GIGGGIGIG+G+PGI +SIGGGIGI  RKGGCKSHDPSKKW+++ + + D  II +A
Sbjct: 1   MTLGIGGGIGIGLGIPGINISIGGGIGIRGRKGGCKSHDPSKKWTEIKSVN-DPAIIEIA 60

Query: 84  NFAVAAFNQKYGNRLVFHAVLEAWVVVLPDGKKEYSIELVAKDCLHRCLYFHAIVIEIVG 143
            FAV  FNQK+GN L F AVLEAWVVV  DGKKEYSIELVAKDCLHRCL +HA+VIEI G
Sbjct: 61  KFAVKVFNQKHGNNLQFQAVLEAWVVVHVDGKKEYSIELVAKDCLHRCLQYHAVVIEICG 120

Query: 144 VPPGWNLFSFTQIID 159
            PPGWNL SF QI++
Sbjct: 121 TPPGWNLVSFNQIVE 134

BLAST of CSPI07G06200 vs. NCBI nr
Match: ADN34263.1 (phloem filament protein [Cucumis melo subsp. melo] >KAA0031424.1 phloem filament protein [Cucumis melo var. makuwa])

HSP 1 Score: 205.3 bits (521), Expect = 3.9e-49
Identity = 97/135 (71.85%), Postives = 112/135 (82.96%), Query Frame = 0

Query: 24  MTIGIGGGIGIGIGVPGIKVSIGGGIGIGSRKGGCKSHDPSKKWSQVPNAHADQVIINVA 83
           MT+GIGGGI IG+G+PGI +SIGGGIGI  RKGGCKSHDPSKKW+++ + + D  II +A
Sbjct: 1   MTLGIGGGISIGLGIPGINISIGGGIGIRGRKGGCKSHDPSKKWTEIKSVN-DPAIIEIA 60

Query: 84  NFAVAAFNQKYGNRLVFHAVLEAWVVVLPDGKKEYSIELVAKDCLHRCLYFHAIVIEIVG 143
            FAV  FNQK+GN L F AVLEAWVVV  DGKKEYSIELVAKDCLHRCL +HA+VIEI G
Sbjct: 61  KFAVKVFNQKHGNNLQFQAVLEAWVVVHVDGKKEYSIELVAKDCLHRCLQYHAVVIEICG 120

Query: 144 VPPGWNLFSFTQIID 159
            PPGWNL SF QI++
Sbjct: 121 TPPGWNLVSFNQIVE 134

BLAST of CSPI07G06200 vs. NCBI nr
Match: ADN34264.1 (phloem filament protein [Cucumis melo subsp. melo] >KAA0031425.1 phloem filament protein [Cucumis melo var. makuwa])

HSP 1 Score: 152.5 bits (384), Expect = 3.0e-33
Identity = 84/136 (61.76%), Postives = 96/136 (70.59%), Query Frame = 0

Query: 24  MTIGIGGGIGIGIGVPGIKVSIGGGIGIGSRKGGCKSHDPSKKWSQVPNAHADQVIINVA 83
           MT+GIGG IGIG G+PG  +SIGGGIGIG RKGGCKSH+PSKKW Q+ N  A  V +  A
Sbjct: 1   MTLGIGGRIGIGFGLPGFNISIGGGIGIGGRKGGCKSHNPSKKWIQLHNVKAPDV-LEAA 60

Query: 84  NFAVAAFNQKYGNRLVFHAVLEAWVVVLPDGKKEYSIELVAKDCLHRCLYFHAIVIE-IV 143
            FAV AFN+KY N L F  V EAWV  + + +KEYSIELVAKDC HR L FHA+VIE  V
Sbjct: 61  KFAVDAFNKKYENNLEFQYVTEAWVHEV-NNEKEYSIELVAKDCFHRLLEFHAVVIEKTV 120

Query: 144 GVPPGWNLFSFTQIID 159
                W L SF +I D
Sbjct: 121 DHKKIWILVSFERIFD 134

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A0A0K4775.8e-8397.47PP1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G067390 PE=4 SV... [more]
A0A5D3C6213.8e-5072.59Phloem filament protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A5A7SJV31.9e-4971.85Phloem filament protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold... [more]
E5GCR51.9e-4971.85Phloem filament protein OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1[more]
A0A5A7SQL41.5e-3361.76Phloem filament protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold... [more]
Match NameE-valueIdentityDescription
KAE8645826.11.8e-7097.12hypothetical protein Csa_017081 [Cucumis sativus][more]
XP_011658840.28.4e-6897.04uncharacterized protein LOC105436098 [Cucumis sativus][more]
TYK06875.17.9e-5072.59phloem filament protein [Cucumis melo var. makuwa][more]
ADN34263.13.9e-4971.85phloem filament protein [Cucumis melo subsp. melo] >KAA0031424.1 phloem filament... [more]
ADN34264.13.0e-3361.76phloem filament protein [Cucumis melo subsp. melo] >KAA0031425.1 phloem filament... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR009994Phloem filament PP1PFAMPF07430PP1coord: 79..140
e-value: 1.4E-9
score: 37.9
NoneNo IPR availableGENE3D3.10.450.10coord: 65..158
e-value: 1.1E-8
score: 36.9
NoneNo IPR availableSUPERFAMILY54403Cystatin/monellincoord: 67..140

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI07G06200.1CSPI07G06200.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010951 negative regulation of endopeptidase activity
molecular_function GO:0004869 cysteine-type endopeptidase inhibitor activity