CSPI07G06040 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI07G06040
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Cystatin domain containing protein
Location	Chr7: 4486004 .. 4486746 (+)
RNA-Seq Expression	CSPI07G06040
Synteny	CSPI07G06040

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CCATTCCTCCCAATAGGTATCAATCTCACAAGGCTCTAGAGCTACACCATTTAGAAGACATCACCATTATTGAGATACACACTTCTATAAATATCAACAGCTACAACAACCAAAATCATCACAAACTAAAACCTTTTGTCCATATACTAAAAATCAAATTTAGGTCACAATGATTTCTACCCTGCTTGCCAACGTTGGTCAACATGATCCCATCCTCCATGTGACGTCAAAGGATACGAAGGGCGATCATCAATCCGAATGGATATCAATTCGTGCTATCGAAGACCCTTATGTGCAAGAAGTTGCGAAATTCGCCATGAGAGAGTTCGAGAAGGAAAAGATGGCAAGGTTTCAATACAAACGGCTGCTAAGTTGTTGGATTAAAGAGGAGATGGGAGGCATAAAATATCGCCTTGTTTTAGAGGTAATTAAAGAAGAAAAGATTGAGGGTAAATATGAGGCTGTTGTATGGGAGGCTTCTGGGCGTTTGATTATAAGGAAGCTTAAGCTTATATCTTTCATGCCTTCTATGCATGGGGCTGCATGACATTTCCATCCCCTCTTTGTATTATTTTCAAACTACAAAGCTTATGGTGTGAAATGCCATTGGTAAAATAAAAAAAAAAAGTATATATATACTTTCTATGTAAAAATCTTCTCTATGTTGATCAAAGCTAACTTTTACTGTACGGGAAATTTACTTACACCTATGGATGTTTGTGTTCAATCAAACAATCATCCAA

mRNA sequence

CCATTCCTCCCAATAGGTATCAATCTCACAAGGCTCTAGAGCTACACCATTTAGAAGACATCACCATTATTGAGATACACACTTCTATAAATATCAACAGCTACAACAACCAAAATCATCACAAACTAAAACCTTTTGTCCATATACTAAAAATCAAATTTAGGTCACAATGATTTCTACCCTGCTTGCCAACGTTGGTCAACATGATCCCATCCTCCATGTGACGTCAAAGGATACGAAGGGCGATCATCAATCCGAATGGATATCAATTCGTGCTATCGAAGACCCTTATGTGCAAGAAGTTGCGAAATTCGCCATGAGAGAGTTCGAGAAGGAAAAGATGGCAAGGTTTCAATACAAACGGCTGCTAAGTTGTTGGATTAAAGAGGAGATGGGAGGCATAAAATATCGCCTTGTTTTAGAGGTAATTAAAGAAGAAAAGATTGAGGGTAAATATGAGGCTGTTGTATGGGAGGCTTCTGGGCGTTTGATTATAAGGAAGCTTAAGCTTATATCTTTCATGCCTTCTATGCATGGGGCTGCATGACATTTCCATCCCCTCTTTGTATTATTTTCAAACTACAAAGCTTATGGTGTGAAATGCCATTGGTAAAATAAAAAAAAAAAGTATATATATACTTTCTATGTAAAAATCTTCTCTATGTTGATCAAAGCTAACTTTTACTGTACGGGAAATTTACTTACACCTATGGATGTTTGTGTTCAATCAAACAATCATCCAA

Coding sequence (CDS)

ATGATTTCTACCCTGCTTGCCAACGTTGGTCAACATGATCCCATCCTCCATGTGACGTCAAAGGATACGAAGGGCGATCATCAATCCGAATGGATATCAATTCGTGCTATCGAAGACCCTTATGTGCAAGAAGTTGCGAAATTCGCCATGAGAGAGTTCGAGAAGGAAAAGATGGCAAGGTTTCAATACAAACGGCTGCTAAGTTGTTGGATTAAAGAGGAGATGGGAGGCATAAAATATCGCCTTGTTTTAGAGGTAATTAAAGAAGAAAAGATTGAGGGTAAATATGAGGCTGTTGTATGGGAGGCTTCTGGGCGTTTGATTATAAGGAAGCTTAAGCTTATATCTTTCATGCCTTCTATGCATGGGGCTGCATGA

Protein sequence

MISTLLANVGQHDPILHVTSKDTKGDHQSEWISIRAIEDPYVQEVAKFAMREFEKEKMARFQYKRLLSCWIKEEMGGIKYRLVLEVIKEEKIEGKYEAVVWEASGRLIIRKLKLISFMPSMHGAA*

Homology

[Close]

BLAST of CSPI07G06040 vs. ExPASy Swiss-Prot
Match: Q10Q47 (Putative cysteine proteinase inhibitor 7 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0210100 PE=3 SV=1)

HSP 1 Score: 46.2 bits (108), Expect = 3.2e-04
Identity = 30/83 (36.14%), Postives = 46/83 (55.42%), Query Frame = 0


Query: 37  IEDPYVQEVAKFAMREFEKEKMARFQYKRLLSCWIKEEMGGIKYRLVLEVIKEEKIEGKY 96
           I DP+VQE+ ++A+ E ++   A       ++   K+ + G+ YRL LE       +G+Y
Sbjct: 35  INDPHVQELGRWAVAEEDRGVAAGGLTFERVTDGEKQVVAGVNYRLTLEASSSGAKDGRY 94

Query: 97  EAVVWEASGRLIIRKLKLISFMP 120
           EAVV+E   R   R  KL+SF P
Sbjct: 95  EAVVYEQDPRSNAR--KLVSFEP 115

[Close]

BLAST of CSPI07G06040 vs. ExPASy TrEMBL
Match: A0A0A0K644 (Cystatin domain containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G066240 PE=4 SV=1)

HSP 1 Score: 250.0 bits (637), Expect = 5.3e-63
Identity = 124/125 (99.20%), Postives = 125/125 (100.00%), Query Frame = 0


Query: 1   MISTLLANVGQHDPILHVTSKDTKGDHQSEWISIRAIEDPYVQEVAKFAMREFEKEKMAR 60
           MISTLLANVGQHDPILHVTSKDTKGDHQSEWISIRAIEDPYVQEVAKFAMREFEKEKMAR
Sbjct: 1   MISTLLANVGQHDPILHVTSKDTKGDHQSEWISIRAIEDPYVQEVAKFAMREFEKEKMAR 60

Query: 61  FQYKRLLSCWIKEEMGGIKYRLVLEVIKEEKIEGKYEAVVWEASGRLIIRKLKLISFMPS 120
           FQYKRLLSCWIKEEMGGIKYRLVLEVIKEEK+EGKYEAVVWEASGRLIIRKLKLISFMPS
Sbjct: 61  FQYKRLLSCWIKEEMGGIKYRLVLEVIKEEKLEGKYEAVVWEASGRLIIRKLKLISFMPS 120

Query: 121 MHGAA 126
           MHGAA
Sbjct: 121 MHGAA 125

[Close]

BLAST of CSPI07G06040 vs. ExPASy TrEMBL
Match: A0A5A7SN35 (Cystatin domain containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G001920 PE=4 SV=1)

HSP 1 Score: 240.4 bits (612), Expect = 4.2e-60
Identity = 117/125 (93.60%), Postives = 122/125 (97.60%), Query Frame = 0


Query: 1   MISTLLANVGQHDPILHVTSKDTKGDHQSEWISIRAIEDPYVQEVAKFAMREFEKEKMAR 60
           MIST+LAN+GQHDPILHVTSKDTKGDHQSEWISIRAIEDPYVQEVAKFA+REFEKEKMAR
Sbjct: 1   MISTVLANIGQHDPILHVTSKDTKGDHQSEWISIRAIEDPYVQEVAKFAVREFEKEKMAR 60

Query: 61  FQYKRLLSCWIKEEMGGIKYRLVLEVIKEEKIEGKYEAVVWEASGRLIIRKLKLISFMPS 120
            QYKRLLSCWIKEEMGGIKYRL+LEVIKEEKIEGKYEA+VWEASGRLIIRKLKLISFMP 
Sbjct: 61  SQYKRLLSCWIKEEMGGIKYRLILEVIKEEKIEGKYEAIVWEASGRLIIRKLKLISFMPC 120

Query: 121 MHGAA 126
           MHG A
Sbjct: 121 MHGVA 125

[Close]

BLAST of CSPI07G06040 vs. ExPASy TrEMBL
Match: A0A2P2NS36 (Cysteine proteinase inhibitor OS=Rhizophora mucronata OX=61149 PE=4 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 2.1e-06
Identity = 38/89 (42.70%), Postives = 53/89 (59.55%), Query Frame = 0


Query: 31  WISIRAIEDPYVQEVAKFAMREFEKEKMARFQYKRLLSCWIKEEMGGIKYRLVLEVIKEE 90
           W  I  + DP+VQE+A +A+RE+     A  + +RL+    K+ + GIKYRLVL  +KE 
Sbjct: 32  WTPITNLSDPHVQEIASYAVREYNHRSNANLKLQRLVK-GEKQVVSGIKYRLVL-AVKEG 91

Query: 91  KIEGKYEAVVWEASGRLIIRKLKLISFMP 120
             + KY+AVVWE +        KLISF P
Sbjct: 92  GSDKKYQAVVWEKAWE---HFRKLISFKP 115

[Close]

BLAST of CSPI07G06040 vs. ExPASy TrEMBL
Match: A0A6J1IC35 (cysteine proteinase inhibitor 5-like OS=Cucurbita maxima OX=3661 GN=LOC111471628 PE=4 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 7.8e-06
Identity = 32/89 (35.96%), Postives = 55/89 (61.80%), Query Frame = 0


Query: 31  WISIRAIEDPYVQEVAKFAMREFEKEKMARFQYKRLLSCWIKEEMGGIKYRLVLEVIKEE 90
           W  I+ ++DP++QE+ KFA+ E+ K+     ++K ++     + + G+ YRLV+E  K E
Sbjct: 31  WEKIKDVKDPHIQEIGKFAVSEYNKQSKGALEFKDVVK-GESQVVSGMNYRLVIEAKKGE 90

Query: 91  KIEGKYEAVVWEASGRLIIRKLKLISFMP 120
            I GKY+A+VWE + +     ++L SF P
Sbjct: 91  SI-GKYQALVWEKAWQ---HFMELTSFKP 114

[Close]

BLAST of CSPI07G06040 vs. ExPASy TrEMBL
Match: A0A6J1EKC1 (cysteine proteinase inhibitor 5-like OS=Cucurbita moschata OX=3662 GN=LOC111434113 PE=4 SV=1)

HSP 1 Score: 59.7 bits (143), Expect = 1.0e-05
Identity = 31/89 (34.83%), Postives = 55/89 (61.80%), Query Frame = 0


Query: 31  WISIRAIEDPYVQEVAKFAMREFEKEKMARFQYKRLLSCWIKEEMGGIKYRLVLEVIKEE 90
           W  I+ ++DP++QE+ KFA+ E+ K+     ++K ++     + + G+ YRLV+E  K E
Sbjct: 31  WEKIKDVKDPHIQEIGKFAVSEYNKQSKGALEFKDVVK-GESQVVSGMNYRLVIEAKKGE 90

Query: 91  KIEGKYEAVVWEASGRLIIRKLKLISFMP 120
            I GKY+A++WE + +     ++L SF P
Sbjct: 91  SI-GKYQALIWEKAWQ---HFMELTSFKP 114

[Close]

BLAST of CSPI07G06040 vs. NCBI nr
Match: KGN43767.1 (hypothetical protein Csa_017208 [Cucumis sativus])

HSP 1 Score: 250.0 bits (637), Expect = 1.1e-62
Identity = 124/125 (99.20%), Postives = 125/125 (100.00%), Query Frame = 0


Query: 1   MISTLLANVGQHDPILHVTSKDTKGDHQSEWISIRAIEDPYVQEVAKFAMREFEKEKMAR 60
           MISTLLANVGQHDPILHVTSKDTKGDHQSEWISIRAIEDPYVQEVAKFAMREFEKEKMAR
Sbjct: 1   MISTLLANVGQHDPILHVTSKDTKGDHQSEWISIRAIEDPYVQEVAKFAMREFEKEKMAR 60

Query: 61  FQYKRLLSCWIKEEMGGIKYRLVLEVIKEEKIEGKYEAVVWEASGRLIIRKLKLISFMPS 120
           FQYKRLLSCWIKEEMGGIKYRLVLEVIKEEK+EGKYEAVVWEASGRLIIRKLKLISFMPS
Sbjct: 61  FQYKRLLSCWIKEEMGGIKYRLVLEVIKEEKLEGKYEAVVWEASGRLIIRKLKLISFMPS 120

Query: 121 MHGAA 126
           MHGAA
Sbjct: 121 MHGAA 125

[Close]

BLAST of CSPI07G06040 vs. NCBI nr
Match: KAA0031436.1 (Cystatin domain containing protein [Cucumis melo var. makuwa] >TYK06887.1 Cystatin domain containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 240.4 bits (612), Expect = 8.7e-60
Identity = 117/125 (93.60%), Postives = 122/125 (97.60%), Query Frame = 0


Query: 1   MISTLLANVGQHDPILHVTSKDTKGDHQSEWISIRAIEDPYVQEVAKFAMREFEKEKMAR 60
           MIST+LAN+GQHDPILHVTSKDTKGDHQSEWISIRAIEDPYVQEVAKFA+REFEKEKMAR
Sbjct: 1   MISTVLANIGQHDPILHVTSKDTKGDHQSEWISIRAIEDPYVQEVAKFAVREFEKEKMAR 60

Query: 61  FQYKRLLSCWIKEEMGGIKYRLVLEVIKEEKIEGKYEAVVWEASGRLIIRKLKLISFMPS 120
            QYKRLLSCWIKEEMGGIKYRL+LEVIKEEKIEGKYEA+VWEASGRLIIRKLKLISFMP 
Sbjct: 61  SQYKRLLSCWIKEEMGGIKYRLILEVIKEEKIEGKYEAIVWEASGRLIIRKLKLISFMPC 120

Query: 121 MHGAA 126
           MHG A
Sbjct: 121 MHGVA 125

[Close]

BLAST of CSPI07G06040 vs. NCBI nr
Match: KAG6588760.1 (hypothetical protein SDJN03_17325, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 182.6 bits (462), Expect = 2.2e-42
Identity = 94/121 (77.69%), Postives = 103/121 (85.12%), Query Frame = 0


Query: 1   MISTLLANVGQHDPILHVTSKDTKGDHQSEWISIRAIEDPYVQEVAKFAMREFEKEKMAR 60
           MISTLL N+GQHDPILHVT KD KGD +SEWISIR IEDPYVQEVAKFA+ EF  +KM  
Sbjct: 1   MISTLLTNIGQHDPILHVT-KDVKGDPRSEWISIRTIEDPYVQEVAKFAVIEFNVDKMTT 60

Query: 61  FQYKRLLSCWIKEEMGGIKYRLVLEVIKEEKIEG--KYEAVVWEASGRLIIRKLKLISFM 120
           FQYKR+L CWIKEEMGGIKYRLVLEV +E   +   KYEAVVWEASGRLIIRKLKL+SF+
Sbjct: 61  FQYKRMLRCWIKEEMGGIKYRLVLEVKQEAPTQKVCKYEAVVWEASGRLIIRKLKLVSFV 120

[Close]

BLAST of CSPI07G06040 vs. NCBI nr
Match: KAG6571859.1 (hypothetical protein SDJN03_28587, partial [Cucurbita argyrosperma subsp. sororia] >KAG7011544.1 hypothetical protein SDJN02_26450, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 181.4 bits (459), Expect = 4.8e-42
Identity = 92/119 (77.31%), Postives = 100/119 (84.03%), Query Frame = 0


Query: 5   LLANVGQHDPILHVTSKDTKGDHQSEWISIRAIEDPYVQEVAKFAMREFEKEKMARFQYK 64
           +LAN+GQHDPILHVT KD KGDH SEWISIRAIEDPYVQEVAKF + E  KEK A FQYK
Sbjct: 1   MLANIGQHDPILHVT-KDAKGDHCSEWISIRAIEDPYVQEVAKFVVVEVNKEKKATFQYK 60

Query: 65  RLLSCWIKEEMGGIKYRLVLEVIKEEKIEGKYEAVVWEASGRLIIRKLKLISFMPSMHG 124
            LL CW+KEEMGGIKYRL+LEV K    EGKYEA+VWEASGRLIIRKLKL+SF+   HG
Sbjct: 61  GLLRCWVKEEMGGIKYRLILEV-KGGASEGKYEAIVWEASGRLIIRKLKLVSFVACNHG 117

[Close]

BLAST of CSPI07G06040 vs. NCBI nr
Match: XP_022973098.1 (cysteine proteinase inhibitor 5-like [Cucurbita maxima] >KAG6583727.1 Cysteine proteinase inhibitor 5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 60.1 bits (144), Expect = 1.6e-05
Identity = 32/89 (35.96%), Postives = 55/89 (61.80%), Query Frame = 0


Query: 31  WISIRAIEDPYVQEVAKFAMREFEKEKMARFQYKRLLSCWIKEEMGGIKYRLVLEVIKEE 90
           W  I+ ++DP++QE+ KFA+ E+ K+     ++K ++     + + G+ YRLV+E  K E
Sbjct: 31  WEKIKDVKDPHIQEIGKFAVSEYNKQSKGALEFKDVVK-GESQVVSGMNYRLVIEAKKGE 90

Query: 91  KIEGKYEAVVWEASGRLIIRKLKLISFMP 120
            I GKY+A+VWE + +     ++L SF P
Sbjct: 91  SI-GKYQALVWEKAWQ---HFMELTSFKP 114

The following BLAST results are available for this feature:

BLAST of CSPI07G06040 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q10Q47	3.2e-04	36.14	Putative cysteine proteinase inhibitor 7 OS=Oryza sativa subsp. japonica OX=3994...	[more]

BLAST of CSPI07G06040 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K644	5.3e-63	99.20	Cystatin domain containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G066240 PE...	[more]
A0A5A7SN35	4.2e-60	93.60	Cystatin domain containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A2P2NS36	2.1e-06	42.70	Cysteine proteinase inhibitor OS=Rhizophora mucronata OX=61149 PE=4 SV=1	[more]
A0A6J1IC35	7.8e-06	35.96	cysteine proteinase inhibitor 5-like OS=Cucurbita maxima OX=3661 GN=LOC111471628...	[more]
A0A6J1EKC1	1.0e-05	34.83	cysteine proteinase inhibitor 5-like OS=Cucurbita moschata OX=3662 GN=LOC1114341...	[more]

BLAST of CSPI07G06040 vs. NCBI nr


Match Name	E-value	Identity	Description
KGN43767.1	1.1e-62	99.20	hypothetical protein Csa_017208 [Cucumis sativus]	[more]
KAA0031436.1	8.7e-60	93.60	Cystatin domain containing protein [Cucumis melo var. makuwa] >TYK06887.1 Cystat...	[more]
KAG6588760.1	2.2e-42	77.69	hypothetical protein SDJN03_17325, partial [Cucurbita argyrosperma subsp. sorori...	[more]
KAG6571859.1	4.8e-42	77.31	hypothetical protein SDJN03_28587, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022973098.1	1.6e-05	35.96	cysteine proteinase inhibitor 5-like [Cucurbita maxima] >KAG6583727.1 Cysteine p...	[more]

BLAST of CSPI07G06040 vs. NCBI nr


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.10.450.10		coord: 25..120 e-value: 4.5E-15 score: 57.6
None	No IPR available	PANTHER	PTHR47364	CYSTEINE PROTEINASE INHIBITOR 5	coord: 21..118
None	No IPR available	PANTHER	PTHR47364:SF2	CYSTEINE PROTEINASE INHIBITOR 5	coord: 21..118
None	No IPR available	SUPERFAMILY	54403	Cystatin/monellin	coord: 31..119
IPR000010	Cystatin domain	PFAM	PF16845	SQAPI	coord: 34..104 e-value: 4.7E-12 score: 46.1

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI07G06040.1	CSPI07G06040.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
molecular_function	GO:0004869	cysteine-type endopeptidase inhibitor activity