CSPI07G05900 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI07G05900
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionProtein kinase domain-containing protein
LocationChr7: 4386043 .. 4387113 (+)
RNA-Seq ExpressionCSPI07G05900
SyntenyCSPI07G05900
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGAATTGGGTTTTAGTAAAGATTTTAGGCCAAGGCTCTGATGGTTTAGTTTGCTTAGCAAAACAAACCACTAGAAAAAAAATCTATAATAATCTTCACTATTATTTTGCTGTCAAACTATCTCCTCTTGGACATAATTCTTCGTTGTGGGAAGAATTAGTATTGAAACAATTCAAGAACTGCCCAGAAATAGTTCAATATTTAGGGAGTGAGATAACTAGATCACCAGGAGGAAGAGATCTCCCTGGTGATAAGGATTTCTACACTTTGAAGTTGGAGTATGCTGCAGGAGGGACTTTGGATGACTTGATCAAACAGAAAGAGAAGTTACCTGAAGATGAAGTGAAAGAATATCTTCGAATGATACTTAAAGGTCTCTCCTGTATTCATTCCAAGGGGTTTGTTCATGTCGATCTTAAGCCTAAGAACATTCTTGCATTCCCTCGAAGTAATGGGAAGATGAAGCTAAAGATTGCTGACTTCGGACATGCTGAGAGATGCAAGTATAGGAATGATGCTGATGCTGATGCTGATGCTGATGATGAGTATAGAAAGTCTAGGCGTCGCAGTTCATTATTGAAGTTTAAAGGAACACCCAGATATATGTCGCCGGAATCCATCGTTTTCGATGAAGTTAACGACGCACACGATGTTTGGTCGCTAGGTTGCGTTTTGGTTAGAATGATTTCTGGAAAATCTGCGTGGGATGAGGTTGCAACTTCTAAACAATTGATGATGAAGATTTTGGATAGCAAGAGCATGCCAAGAATACCTGAGGAGTTGTCCATACAAGGTAAGGACTTCCTCAAAAAATGCTTTGTTTGGAATTTTAAACAACGGTGGACGGCTGATATGTTACTCCAACATCCCTATCTCAAAGAAAAACATGAAGCTACGACGATGAAAGATGATGATGGGATGCCTAAACTTGCCAAATCGCTTTTGTTGTCTCATCAACCATTGAAGATCAGCCAGACTCGATCAAGTAGTGATCATGATAGAAAATCCAATCTTCGAGAAAAGAATTATTTGTCTCCTATATTAAGAAGGATTAAATCCCGTAAGTAA

mRNA sequence

ATGGAGAATTGGGTTTTAGTAAAGATTTTAGGCCAAGGCTCTGATGGTTTAGTTTGCTTAGCAAAACAAACCACTAGAAAAAAAATCTATAATAATCTTCACTATTATTTTGCTGTCAAACTATCTCCTCTTGGACATAATTCTTCGTTGTGGGAAGAATTAGTATTGAAACAATTCAAGAACTGCCCAGAAATAGTTCAATATTTAGGGAGTGAGATAACTAGATCACCAGGAGGAAGAGATCTCCCTGGTGATAAGGATTTCTACACTTTGAAGTTGGAGTATGCTGCAGGAGGGACTTTGGATGACTTGATCAAACAGAAAGAGAAGTTACCTGAAGATGAAGTGAAAGAATATCTTCGAATGATACTTAAAGGTCTCTCCTGTATTCATTCCAAGGGGTTTGTTCATGTCGATCTTAAGCCTAAGAACATTCTTGCATTCCCTCGAAGTAATGGGAAGATGAAGCTAAAGATTGCTGACTTCGGACATGCTGAGAGATGCAAGTATAGGAATGATGCTGATGCTGATGCTGATGCTGATGATGAGTATAGAAAGTCTAGGCGTCGCAGTTCATTATTGAAGTTTAAAGGAACACCCAGATATATGTCGCCGGAATCCATCGTTTTCGATGAAGTTAACGACGCACACGATGTTTGGTCGCTAGGTTGCGTTTTGGTTAGAATGATTTCTGGAAAATCTGCGTGGGATGAGGTTGCAACTTCTAAACAATTGATGATGAAGATTTTGGATAGCAAGAGCATGCCAAGAATACCTGAGGAGTTGTCCATACAAGGTAAGGACTTCCTCAAAAAATGCTTTGTTTGGAATTTTAAACAACGGTGGACGGCTGATATGTTACTCCAACATCCCTATCTCAAAGAAAAACATGAAGCTACGACGATGAAAGATGATGATGGGATGCCTAAACTTGCCAAATCGCTTTTGTTGTCTCATCAACCATTGAAGATCAGCCAGACTCGATCAAGTAGTGATCATGATAGAAAATCCAATCTTCGAGAAAAGAATTATTTGTCTCCTATATTAAGAAGGATTAAATCCCGTAAGTAA

Coding sequence (CDS)

ATGGAGAATTGGGTTTTAGTAAAGATTTTAGGCCAAGGCTCTGATGGTTTAGTTTGCTTAGCAAAACAAACCACTAGAAAAAAAATCTATAATAATCTTCACTATTATTTTGCTGTCAAACTATCTCCTCTTGGACATAATTCTTCGTTGTGGGAAGAATTAGTATTGAAACAATTCAAGAACTGCCCAGAAATAGTTCAATATTTAGGGAGTGAGATAACTAGATCACCAGGAGGAAGAGATCTCCCTGGTGATAAGGATTTCTACACTTTGAAGTTGGAGTATGCTGCAGGAGGGACTTTGGATGACTTGATCAAACAGAAAGAGAAGTTACCTGAAGATGAAGTGAAAGAATATCTTCGAATGATACTTAAAGGTCTCTCCTGTATTCATTCCAAGGGGTTTGTTCATGTCGATCTTAAGCCTAAGAACATTCTTGCATTCCCTCGAAGTAATGGGAAGATGAAGCTAAAGATTGCTGACTTCGGACATGCTGAGAGATGCAAGTATAGGAATGATGCTGATGCTGATGCTGATGCTGATGATGAGTATAGAAAGTCTAGGCGTCGCAGTTCATTATTGAAGTTTAAAGGAACACCCAGATATATGTCGCCGGAATCCATCGTTTTCGATGAAGTTAACGACGCACACGATGTTTGGTCGCTAGGTTGCGTTTTGGTTAGAATGATTTCTGGAAAATCTGCGTGGGATGAGGTTGCAACTTCTAAACAATTGATGATGAAGATTTTGGATAGCAAGAGCATGCCAAGAATACCTGAGGAGTTGTCCATACAAGGTAAGGACTTCCTCAAAAAATGCTTTGTTTGGAATTTTAAACAACGGTGGACGGCTGATATGTTACTCCAACATCCCTATCTCAAAGAAAAACATGAAGCTACGACGATGAAAGATGATGATGGGATGCCTAAACTTGCCAAATCGCTTTTGTTGTCTCATCAACCATTGAAGATCAGCCAGACTCGATCAAGTAGTGATCATGATAGAAAATCCAATCTTCGAGAAAAGAATTATTTGTCTCCTATATTAAGAAGGATTAAATCCCGTAAGTAA

Protein sequence

MENWVLVKILGQGSDGLVCLAKQTTRKKIYNNLHYYFAVKLSPLGHNSSLWEELVLKQFKNCPEIVQYLGSEITRSPGGRDLPGDKDFYTLKLEYAAGGTLDDLIKQKEKLPEDEVKEYLRMILKGLSCIHSKGFVHVDLKPKNILAFPRSNGKMKLKIADFGHAERCKYRNDADADADADDEYRKSRRRSSLLKFKGTPRYMSPESIVFDEVNDAHDVWSLGCVLVRMISGKSAWDEVATSKQLMMKILDSKSMPRIPEELSIQGKDFLKKCFVWNFKQRWTADMLLQHPYLKEKHEATTMKDDDGMPKLAKSLLLSHQPLKISQTRSSSDHDRKSNLREKNYLSPILRRIKSRK*
Homology
BLAST of CSPI07G05900 vs. ExPASy Swiss-Prot
Match: Q9SND6 (Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK20 PE=1 SV=1)

HSP 1 Score: 167.5 bits (423), Expect = 2.7e-40
Identity = 118/315 (37.46%), Postives = 171/315 (54.29%), Query Frame = 0

Query: 4   WVLVKILGQGSDGLVCLAKQTTRKKIYNNLHYYFAVKLSPLGHNSSLW-EELVLKQFKNC 63
           WV  + +G G+   V  A   T+ +   +     AVK +     +SL  E+ VL    +C
Sbjct: 3   WVRGETIGFGTFSTVSTA---TKSRNSGDFPALIAVKSTDAYGAASLSNEKSVLDSLGDC 62

Query: 64  PEIVQYLGSEITRSPGGRDLPGDKDFYTLKLEYAAGGTLDDLIKQ--KEKLPEDEVKEYL 123
           PEI++  G + T   G       ++ + L LEYA+ G+L   +K+   E LPE  V+ + 
Sbjct: 63  PEIIRCYGEDSTVENG-------EEMHNLLLEYASRGSLASYMKKLGGEGLPESTVRRHT 122

Query: 124 RMILKGLSCIHSKGFVHVDLKPKNILAFPRSNGKMKLKIADFGHAERCKYRNDADADADA 183
             +L+GL  IH+KGF H D+K  NIL F  ++G   +KIADFG A R       D D  A
Sbjct: 123 GSVLRGLRHIHAKGFAHCDIKLANILLF--NDG--SVKIADFGLAMR------VDGDLTA 182

Query: 184 DDEYRKSRRRSSLLKFKGTPRYMSPESIVFDEVNDAHDVWSLGCVLVRMISGKSAWD--E 243
               RKS      ++ +GTP YM+PE +  +E   A DVW+LGC +V M SGK+AW   E
Sbjct: 183 ---LRKS------VEIRGTPLYMAPECVNDNEYGSAADVWALGCAVVEMFSGKTAWSVKE 242

Query: 244 VATSKQLMMKILDSKSMPRIPEELSIQGKDFLKKCFVWNFKQRWTADMLLQHPY----LK 303
            +    L+++I     +P+IPE LS +GKDFL KCFV +  +RWTA+MLL H +    L+
Sbjct: 243 GSHFMSLLIRIGVGDELPKIPEMLSEEGKDFLSKCFVKDPAKRWTAEMLLNHSFVTIDLE 288

Query: 304 EKHE---ATTMKDDD 307
           + H       +KD+D
Sbjct: 303 DDHRENFVVKVKDED 288

BLAST of CSPI07G05900 vs. ExPASy Swiss-Prot
Match: O80888 (Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK17 PE=1 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 2.3e-31
Identity = 94/297 (31.65%), Postives = 139/297 (46.80%), Query Frame = 0

Query: 4   WVLVKILGQGSDGLVCLAKQTTRKKIYNNLHYYFAVKLSPLGHNSSLWEELVLKQFKNCP 63
           W   +ILG+GS   V  A         +N     AVK S +  +  L  E  +    + P
Sbjct: 3   WTRGRILGRGSTATVYAAAG-------HNSDEILAVKSSEVHRSEFLQREAKILSSLSSP 62

Query: 64  EIVQYLGSEITRSPGGRDLPGDKDFYTLKLEYAAGGTLDD-LIKQKEKLPEDEVKEYLRM 123
            ++ Y GSE  R   G  +      Y L +EYA  GTL D   K   ++ E  V +Y R 
Sbjct: 63  YVIGYRGSETKRESNGVVM------YNLLMEYAPYGTLTDAAAKDGGRVDETRVVKYTRD 122

Query: 124 ILKGLSCIHSKGFVHVDLKPKNILAFPRSNGKMKLKIADFGHAERCKYRNDADADADADD 183
           ILKGL  IHSKG VH D+K  N++     + K + KIADFG A+R             D 
Sbjct: 123 ILKGLEYIHSKGIVHCDVKGSNVVI----SEKGEAKIADFGCAKR------------VDP 182

Query: 184 EYRKSRRRSSLLKFKGTPRYMSPESIVFDEVNDAHDVWSLGCVLVRMISGKSAWDEVATS 243
            +             GTP +M+PE    ++     D+W++GC ++ M++G   W +  + 
Sbjct: 183 VFES--------PVMGTPAFMAPEVARGEKQGKESDIWAVGCTMIEMVTGSPPWTKADSR 242

Query: 244 K---QLMMKILDSKSMPRIPEELSIQGKDFLKKCFVWNFKQRWTADMLLQHPYLKEK 297
           +    ++ ++  S   P +P  L+ + KDFL+KC      +RWTA  LL HP+L  K
Sbjct: 243 EDPVSVLYRVGYSSETPELPCLLAEEAKDFLEKCLKREANERWTATQLLNHPFLTTK 262

BLAST of CSPI07G05900 vs. ExPASy Swiss-Prot
Match: O22040 (Mitogen-activated protein kinase kinase kinase ANP1 OS=Arabidopsis thaliana OX=3702 GN=ANP1 PE=1 SV=2)

HSP 1 Score: 134.8 bits (338), Expect = 1.9e-30
Identity = 95/334 (28.44%), Postives = 158/334 (47.31%), Query Frame = 0

Query: 3   NWVLVKILGQGSDGLVCLAKQTTRKKIYN----NLHYYFAVKLSPLGHNSSLWEELVLKQ 62
           +W   +++G+G+ G V +       ++       +   FA K     H   L EE+ L +
Sbjct: 68  SWRKGQLIGRGAFGTVYMGMNLDSGELLAVKQVLIAANFASKEKTQAHIQELEEEVKLLK 127

Query: 63  FKNCPEIVQYLGSEITRSPGGRDLPGDKDFYTLKLEYAAGGTLDDLIKQKEKLPEDEVKE 122
             + P IV+YLG+             + D   + LE+  GG++  L+++    PE  V+ 
Sbjct: 128 NLSHPNIVRYLGT-----------VREDDTLNILLEFVPGGSISSLLEKFGPFPESVVRT 187

Query: 123 YLRMILKGLSCIHSKGFVHVDLKPKNILAFPRSNGKMKLKIADFGHAERCKYRNDADADA 182
           Y R +L GL  +H+   +H D+K  NIL     + K  +K+ADFG               
Sbjct: 188 YTRQLLLGLEYLHNHAIMHRDIKGANILV----DNKGCIKLADFG--------------- 247

Query: 183 DADDEYRKSRRRSSLLKFKGTPRYMSPESIVFDEVNDAHDVWSLGCVLVRMISGKSAWDE 242
            A  +  +    +     KGTP +M+PE I+    + + D+WS+GC ++ M++GK+ W +
Sbjct: 248 -ASKQVAELATMTGAKSMKGTPYWMAPEVILQTGHSFSADIWSVGCTVIEMVTGKAPWSQ 307

Query: 243 VATSKQLMMKILDSKSMPRIPEELSIQGKDFLKKCFVWNFKQRWTADMLLQHPYLKEKHE 302
                  +  I  +KS P IP+ LS   KDFL KC       R TA  LL+HP++  KH+
Sbjct: 308 QYKEVAAIFFIGTTKSHPPIPDTLSSDAKDFLLKCLQEVPNLRPTASELLKHPFVMGKHK 366

Query: 303 ATTMKDDDGMPKLAKSLLLSHQPLKISQTRSSSD 333
            +   D   +     + L +  PL+I+ T+S+ D
Sbjct: 368 ESASTDLGSV----LNNLSTPLPLQINNTKSTPD 366

BLAST of CSPI07G05900 vs. ExPASy Swiss-Prot
Match: F4HRJ4 (Mitogen-activated protein kinase kinase kinase 3 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK3 PE=1 SV=1)

HSP 1 Score: 130.2 bits (326), Expect = 4.7e-29
Identity = 84/249 (33.73%), Postives = 125/249 (50.20%), Query Frame = 0

Query: 63  PEIVQYLGSEITRSPGGRDLPGDKDFYTLKLEYAAGGTLDDLIKQKEKLPEDEVKEYLRM 122
           P IVQY GSE++           ++  ++ LEY +GG++  L+K      E  ++ Y R 
Sbjct: 274 PNIVQYYGSELS-----------EETLSVYLEYVSGGSIHKLLKDYGSFTEPVIQNYTRQ 333

Query: 123 ILKGLSCIHSKGFVHVDLKPKNILAFPRSNGKMKLKIADFGHAERCKYRNDADADADADD 182
           IL GL+ +H +  VH D+K  NIL  P  NG++KL  ADFG A+                
Sbjct: 334 ILAGLAYLHGRNTVHRDIKGANILVDP--NGEIKL--ADFGMAKHV-------------- 393

Query: 183 EYRKSRRRSSLLKFKGTPRYMSPESIVFDE-VNDAHDVWSLGCVLVRMISGKSAWDEVAT 242
                   S++L FKG+P +M+PE ++       A D+WSLGC ++ M + K  W +   
Sbjct: 394 -----TAFSTMLSFKGSPYWMAPEVVMSQNGYTHAVDIWSLGCTILEMATSKPPWSQF-E 453

Query: 243 SKQLMMKILDSKSMPRIPEELSIQGKDFLKKCFVWNFKQRWTADMLLQHPYLKEKHEATT 302
               + KI +SK  P IP+ LS   K+F++ C   N   R TA  LL+HP+L+     TT
Sbjct: 454 GVAAIFKIGNSKDTPEIPDHLSNDAKNFIRLCLQRNPTVRPTASQLLEHPFLRN----TT 483

Query: 303 MKDDDGMPK 311
                 +PK
Sbjct: 514 RVASTSLPK 483

BLAST of CSPI07G05900 vs. ExPASy Swiss-Prot
Match: Q54R82 (Mitogen-activated protein kinase kinase kinase A OS=Dictyostelium discoideum OX=44689 GN=mkkA PE=1 SV=2)

HSP 1 Score: 128.6 bits (322), Expect = 1.4e-28
Identity = 96/349 (27.51%), Postives = 163/349 (46.70%), Query Frame = 0

Query: 4   WVLVKILGQGSDGLVCLAKQTTRKKIYNNLHYYFAVK-LSPLGHNS---------SLWEE 63
           W   +ILG+G  G V L       ++       FAVK L  +  NS         S  +E
Sbjct: 170 WQKGQILGRGGYGSVYLGLNKDTGEL-------FAVKQLEIVDINSDPKLKNMILSFSKE 229

Query: 64  LVLKQFKNCPEIVQYLGSEITRSPGGRDLPGDKDFYTLKLEYAAGGTLDDLIKQKEKLPE 123
           + + +      IV+YLG+ +           D+ F ++ LEY  GG++  L+ +     E
Sbjct: 230 IEVMRSLRHDNIVRYLGTSL-----------DQSFLSVFLEYIPGGSISSLLGKFGAFSE 289

Query: 124 DEVKEYLRMILKGLSCIHSKGFVHVDLKPKNILAFPRSNGKMKLKIADFGHAERCKYRND 183
           + +K Y + IL+GLS +H+   +H D+K  NIL     + K  +K++DFG ++       
Sbjct: 290 NVIKVYTKQILQGLSFLHANSIIHRDIKGANILI----DTKGIVKLSDFGCSKSF----- 349

Query: 184 ADADADADDEYRKSRRRSSLLKFKGTPRYMSPESIVFDEVNDAHDVWSLGCVLVRMISGK 243
                        S   S     +GTP +M+PE I       + D+WSLGCV+V M + +
Sbjct: 350 -------------SGIVSQFKSMQGTPYWMAPEVIKQTGHGRSSDIWSLGCVIVEMATAQ 409

Query: 244 SAWDEVATSKQLMMKILDSKSMPRIPEELSIQGKDFLKKCFVWNFKQRWTADMLLQHPYL 303
             W  +     +M  I  S S+P IP  +S +  DFL  CF  + K+R  A+ LL+HP++
Sbjct: 410 PPWSNITELAAVMYHIASSNSIPNIPSHMSQEAFDFLNLCFKRDPKERPDANQLLKHPFI 469

Query: 304 KEKHEATTMKDDDGMPKLAKSLLLSHQPLKISQTRSSSDHDRKSNLREK 343
                   + D+  +P ++ +  LS      + T +++++   SN+ ++
Sbjct: 470 ------MNLDDNIQLPTISPTTTLSTNTTNTTATTTTTNNATNSNINQQ 472

BLAST of CSPI07G05900 vs. ExPASy TrEMBL
Match: A0A0A0K7G3 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G065150 PE=4 SV=1)

HSP 1 Score: 709.9 bits (1831), Expect = 5.3e-201
Identity = 350/356 (98.31%), Postives = 352/356 (98.88%), Query Frame = 0

Query: 1   MENWVLVKILGQGSDGLVCLAKQTTRKKIYNNLHYYFAVKLSPLGHNSSLWEELVLKQFK 60
           MENWVLVKILGQGSDGLVCLAKQTTRKKIYNNLHYYFAVKLSPLGHNSSLWEELVLKQFK
Sbjct: 1   MENWVLVKILGQGSDGLVCLAKQTTRKKIYNNLHYYFAVKLSPLGHNSSLWEELVLKQFK 60

Query: 61  NCPEIVQYLGSEITRSPGGRDLPGDKDFYTLKLEYAAGGTLDDLIKQKEKLPEDEVKEYL 120
           NCPEIVQYLGSEITRSPGGRDLPGDKDFYTLKLEYAAGGTLDDLIKQKEKLPEDEVKEYL
Sbjct: 61  NCPEIVQYLGSEITRSPGGRDLPGDKDFYTLKLEYAAGGTLDDLIKQKEKLPEDEVKEYL 120

Query: 121 RMILKGLSCIHSKGFVHVDLKPKNILAFPRSNGKMKLKIADFGHAERCKYRNDADADADA 180
           RMILKGLSCIHSKGFVHVDLKPKNILAFPRSNGKMKLKIADFGHAERCKYRN  DADA +
Sbjct: 121 RMILKGLSCIHSKGFVHVDLKPKNILAFPRSNGKMKLKIADFGHAERCKYRN--DADAHS 180

Query: 181 DDEYRKSRRRSSLLKFKGTPRYMSPESIVFDEVNDAHDVWSLGCVLVRMISGKSAWDEVA 240
           DDEYRKSRRRSSLLKFKGTPRYMSPESIVFDEVNDAHDVWSLGCVLVRMISGKSAWDEVA
Sbjct: 181 DDEYRKSRRRSSLLKFKGTPRYMSPESIVFDEVNDAHDVWSLGCVLVRMISGKSAWDEVA 240

Query: 241 TSKQLMMKILDSKSMPRIPEELSIQGKDFLKKCFVWNFKQRWTADMLLQHPYLKEKHEAT 300
           TSKQLMMKILDSKSMPRIPEELSIQGKDF+KKCFVWNFKQRWTADMLLQHPYL EKHEAT
Sbjct: 241 TSKQLMMKILDSKSMPRIPEELSIQGKDFIKKCFVWNFKQRWTADMLLQHPYLNEKHEAT 300

Query: 301 TMKDDDGMPKLAKSLLLSHQPLKISQTRSSSDHDRKSNLREKNYLSPILRRIKSRK 357
           TMKDDDGMPKLAKSLLLSHQPLKISQTRSSSDHDRKSNLREKNYLSPILRRIKSRK
Sbjct: 301 TMKDDDGMPKLAKSLLLSHQPLKISQTRSSSDHDRKSNLREKNYLSPILRRIKSRK 354

BLAST of CSPI07G05900 vs. ExPASy TrEMBL
Match: A0A5D3C4R6 (Mitogen-activated protein kinase kinase kinase A-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G002080 PE=4 SV=1)

HSP 1 Score: 510.8 bits (1314), Expect = 4.7e-141
Identity = 274/363 (75.48%), Postives = 304/363 (83.75%), Query Frame = 0

Query: 1   MENWVLVKILGQGSDGLVCLAKQTTRKKIYNNLHYYFAVKLSPLGHNSS-LWEELVLKQF 60
           ME WV VK+LGQGSDG+V LAKQT R+KI  NLHYYFAVKLSPL HNSS LWEE VLKQF
Sbjct: 1   MEKWVFVKVLGQGSDGVVWLAKQTIRRKIGRNLHYYFAVKLSPLRHNSSLLWEEQVLKQF 60

Query: 61  KNCPEIVQYLGSEITRSPGGRDLPGDKDFYTLKLEYAAGGTLDDLIKQKEKLPEDEVKEY 120
           KNCPEIVQYLGSEITR   G DLP DKDFYTLKLEYAAGGTLDDLIKQKEKLPE+EVK+Y
Sbjct: 61  KNCPEIVQYLGSEITR---GGDLPQDKDFYTLKLEYAAGGTLDDLIKQKEKLPENEVKDY 120

Query: 121 LRMILKGLSCIHSKGFVHVDLKPKNILAFPRSNGKMKLKIADFGHAERCK-YRNDADADA 180
           LRMILKGLSC+HSKGFVHVDLKP NILAFP+S+GKMKLKIADFGHAERCK YRN    D 
Sbjct: 121 LRMILKGLSCVHSKGFVHVDLKPNNILAFPQSDGKMKLKIADFGHAERCKLYRN----DD 180

Query: 181 DADDEYRKSRRRSSLLKFKGTPRYMSPESIVFDEVNDAHDVWSLGCVLVRMISGKSAWDE 240
           D DD+ RK     S LKFKGTPRYMSPESIV +EVNDAHD+WSLGCVLV+MISGKS WD+
Sbjct: 181 DDDDQDRKYGGYCSSLKFKGTPRYMSPESIVCNEVNDAHDIWSLGCVLVKMISGKSVWDD 240

Query: 241 VATSKQLMMKILDSK--SMPRIPEELSIQGKDFLKKCFVWNFKQRWTADMLLQHPYL--K 300
           +  S+QLMM++LD+K  +MP IP+ELS QGKDF+KKCF+ N KQRWTADMLLQHPYL  +
Sbjct: 241 LIDSRQLMMRLLDNKNYNMPTIPKELSKQGKDFIKKCFIRNCKQRWTADMLLQHPYLMHE 300

Query: 301 EKHEATTMKDDDGMP-KLAKSLLLSHQ-PLKISQTRSS---SDHDRKSNLREKNYLSPIL 353
           E   ATT+KDDD MP  LAK+L LS Q PLK SQTRSS   ++++RK NLR KNYL P+L
Sbjct: 301 ENEAATTVKDDDQMPTTLAKALSLSRQRPLK-SQTRSSNNNNNNNRKFNLRAKNYLLPLL 355

BLAST of CSPI07G05900 vs. ExPASy TrEMBL
Match: A0A1S3C1U9 (mitogen-activated protein kinase kinase kinase A-like OS=Cucumis melo OX=3656 GN=LOC103495681 PE=4 SV=1)

HSP 1 Score: 510.8 bits (1314), Expect = 4.7e-141
Identity = 274/363 (75.48%), Postives = 304/363 (83.75%), Query Frame = 0

Query: 1   MENWVLVKILGQGSDGLVCLAKQTTRKKIYNNLHYYFAVKLSPLGHNSS-LWEELVLKQF 60
           ME WV VK+LGQGSDG+V LAKQT R+KI  NLHYYFAVKLSPL HNSS LWEE VLKQF
Sbjct: 1   MEKWVFVKVLGQGSDGVVWLAKQTIRRKIGRNLHYYFAVKLSPLRHNSSLLWEEQVLKQF 60

Query: 61  KNCPEIVQYLGSEITRSPGGRDLPGDKDFYTLKLEYAAGGTLDDLIKQKEKLPEDEVKEY 120
           KNCPEIVQYLGSEITR   G DLP DKDFYTLKLEYAAGGTLDDLIKQKEKLPE+EVK+Y
Sbjct: 61  KNCPEIVQYLGSEITR---GGDLPQDKDFYTLKLEYAAGGTLDDLIKQKEKLPENEVKDY 120

Query: 121 LRMILKGLSCIHSKGFVHVDLKPKNILAFPRSNGKMKLKIADFGHAERCK-YRNDADADA 180
           LRMILKGLSC+HSKGFVHVDLKP NILAFP+S+GKMKLKIADFGHAERCK YRN    D 
Sbjct: 121 LRMILKGLSCVHSKGFVHVDLKPNNILAFPQSDGKMKLKIADFGHAERCKLYRN----DD 180

Query: 181 DADDEYRKSRRRSSLLKFKGTPRYMSPESIVFDEVNDAHDVWSLGCVLVRMISGKSAWDE 240
           D DD+ RK     S LKFKGTPRYMSPESIV +EVNDAHD+WSLGCVLV+MISGKS WD+
Sbjct: 181 DDDDQDRKYGGYCSSLKFKGTPRYMSPESIVCNEVNDAHDIWSLGCVLVKMISGKSVWDD 240

Query: 241 VATSKQLMMKILDSK--SMPRIPEELSIQGKDFLKKCFVWNFKQRWTADMLLQHPYL--K 300
           +  S+QLMM++LD+K  +MP IP+ELS QGKDF+KKCF+ N KQRWTADMLLQHPYL  +
Sbjct: 241 LIDSRQLMMRLLDNKNYNMPTIPKELSKQGKDFIKKCFIRNCKQRWTADMLLQHPYLMHE 300

Query: 301 EKHEATTMKDDDGMP-KLAKSLLLSHQ-PLKISQTRSS---SDHDRKSNLREKNYLSPIL 353
           E   ATT+KDDD MP  LAK+L LS Q PLK SQTRSS   ++++RK NLR KNYL P+L
Sbjct: 301 ENEAATTVKDDDQMPTTLAKALSLSRQRPLK-SQTRSSNNNNNNNRKFNLRAKNYLLPLL 355

BLAST of CSPI07G05900 vs. ExPASy TrEMBL
Match: A0A5D3DR57 (Mitogen-activated protein kinase kinase kinase ANP1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold111G00640 PE=4 SV=1)

HSP 1 Score: 442.2 bits (1136), Expect = 2.1e-120
Identity = 227/317 (71.61%), Postives = 261/317 (82.33%), Query Frame = 0

Query: 1   MENWVLVKILGQGSDGLVCLAKQTT--RKKIYNNLHYYFAVKLSPLGHNSS-LWEELVLK 60
           MENWV VK+LGQGS G+VCLAKQ++  RKK  +NL YYFAVKLSPL HNSS LWEE VLK
Sbjct: 1   MENWVPVKVLGQGSYGVVCLAKQSSAIRKKSDSNLPYYFAVKLSPLQHNSSLLWEEQVLK 60

Query: 61  QFKNCPEIVQYLGSEITRSPGGRDLPGDK-DFYTLKLEYAAGGTLDDLIKQKEKLPEDEV 120
           QFK+CPEIVQYLGSEITR   GRD+  D  DFYTLKLEYAAGGTLDDLI+Q++KLPE EV
Sbjct: 61  QFKDCPEIVQYLGSEITR---GRDIRNDHVDFYTLKLEYAAGGTLDDLIEQRDKLPEAEV 120

Query: 121 KEYLRMILKGLSCIHSKGFVHVDLKPKNILAFPRSNGKMKLKIADFGHAERCKYRNDADA 180
           K+YLRMILKGLSCIHSKGFVHVDLKP NILAFP+S+GKMKLKIADFG AER KYR     
Sbjct: 121 KDYLRMILKGLSCIHSKGFVHVDLKPNNILAFPQSDGKMKLKIADFGQAERSKYR----- 180

Query: 181 DADADDEYRKSRRRSSLLKFKGTPRYMSPESIVFDEVNDAHDVWSLGCVLVRMISGKSAW 240
               DD++++     S  KFKGTPRYMSPES+VF+EVNDA D+WSLGC+LV+MISGKSAW
Sbjct: 181 ---VDDQHKQYPPCCSSFKFKGTPRYMSPESVVFNEVNDADDIWSLGCILVKMISGKSAW 240

Query: 241 DEVATSKQLMMKILDSKSMPRIPEELSIQGKDFLKKCFVWNFKQRWTADMLLQHPYLKEK 300
           D+   SKQLM+K+LD+K MP IP ELS QGKDF+KKCF+ N+K+RWTA+MLLQHPYL E+
Sbjct: 241 DDCKDSKQLMIKLLDNKIMPTIPGELSEQGKDFIKKCFIRNYKERWTAEMLLQHPYLNEE 300

Query: 301 HEA-------TTMKDDD 307
           +EA        TMK D+
Sbjct: 301 NEAPMNNKNEATMKKDN 306

BLAST of CSPI07G05900 vs. ExPASy TrEMBL
Match: A0A1S3CQQ0 (mitogen-activated protein kinase kinase kinase ANP1-like OS=Cucumis melo OX=3656 GN=LOC103503238 PE=4 SV=1)

HSP 1 Score: 441.8 bits (1135), Expect = 2.7e-120
Identity = 227/317 (71.61%), Postives = 260/317 (82.02%), Query Frame = 0

Query: 1   MENWVLVKILGQGSDGLVCLAKQTT--RKKIYNNLHYYFAVKLSPLGHNSS-LWEELVLK 60
           MENWV VK+LGQGS  +VCLAKQ++  RKK  NNL YYFAVKLSPL HNSS LWEE VLK
Sbjct: 1   MENWVPVKVLGQGSYAVVCLAKQSSAIRKKSDNNLPYYFAVKLSPLQHNSSLLWEEQVLK 60

Query: 61  QFKNCPEIVQYLGSEITRSPGGRDLPGDK-DFYTLKLEYAAGGTLDDLIKQKEKLPEDEV 120
           QFK+CPEIVQYLGSEITR   GRD+  D  DFYTLKLEYAAGGTLDDLI+Q++KLPE EV
Sbjct: 61  QFKDCPEIVQYLGSEITR---GRDIRNDHVDFYTLKLEYAAGGTLDDLIEQRDKLPEAEV 120

Query: 121 KEYLRMILKGLSCIHSKGFVHVDLKPKNILAFPRSNGKMKLKIADFGHAERCKYRNDADA 180
           K+YLRMILKGLSCIHSKGFVHVDLKP NILAFP+S+GKMKLKIADFG AER KYR     
Sbjct: 121 KDYLRMILKGLSCIHSKGFVHVDLKPNNILAFPQSDGKMKLKIADFGQAERSKYR----- 180

Query: 181 DADADDEYRKSRRRSSLLKFKGTPRYMSPESIVFDEVNDAHDVWSLGCVLVRMISGKSAW 240
               DD++++     S  KFKGTPRYMSPES+VF+EVNDA D+WSLGC+LV+MISGKSAW
Sbjct: 181 ---VDDQHKQYPPCCSSFKFKGTPRYMSPESVVFNEVNDADDIWSLGCILVKMISGKSAW 240

Query: 241 DEVATSKQLMMKILDSKSMPRIPEELSIQGKDFLKKCFVWNFKQRWTADMLLQHPYLKEK 300
           D+   SKQLM+K+LD+K MP IP ELS QGKDF+KKCF+ N+K+RWTA+MLLQHPYL E+
Sbjct: 241 DDCKDSKQLMIKLLDNKIMPTIPGELSEQGKDFIKKCFIRNYKERWTAEMLLQHPYLNEE 300

Query: 301 HEA-------TTMKDDD 307
           +EA        TMK D+
Sbjct: 301 NEAPMNNKNEATMKKDN 306

BLAST of CSPI07G05900 vs. NCBI nr
Match: XP_004137063.1 (mitogen-activated protein kinase kinase kinase 17 [Cucumis sativus] >KGN43756.1 hypothetical protein Csa_017187 [Cucumis sativus])

HSP 1 Score: 709.9 bits (1831), Expect = 1.1e-200
Identity = 350/356 (98.31%), Postives = 352/356 (98.88%), Query Frame = 0

Query: 1   MENWVLVKILGQGSDGLVCLAKQTTRKKIYNNLHYYFAVKLSPLGHNSSLWEELVLKQFK 60
           MENWVLVKILGQGSDGLVCLAKQTTRKKIYNNLHYYFAVKLSPLGHNSSLWEELVLKQFK
Sbjct: 1   MENWVLVKILGQGSDGLVCLAKQTTRKKIYNNLHYYFAVKLSPLGHNSSLWEELVLKQFK 60

Query: 61  NCPEIVQYLGSEITRSPGGRDLPGDKDFYTLKLEYAAGGTLDDLIKQKEKLPEDEVKEYL 120
           NCPEIVQYLGSEITRSPGGRDLPGDKDFYTLKLEYAAGGTLDDLIKQKEKLPEDEVKEYL
Sbjct: 61  NCPEIVQYLGSEITRSPGGRDLPGDKDFYTLKLEYAAGGTLDDLIKQKEKLPEDEVKEYL 120

Query: 121 RMILKGLSCIHSKGFVHVDLKPKNILAFPRSNGKMKLKIADFGHAERCKYRNDADADADA 180
           RMILKGLSCIHSKGFVHVDLKPKNILAFPRSNGKMKLKIADFGHAERCKYRN  DADA +
Sbjct: 121 RMILKGLSCIHSKGFVHVDLKPKNILAFPRSNGKMKLKIADFGHAERCKYRN--DADAHS 180

Query: 181 DDEYRKSRRRSSLLKFKGTPRYMSPESIVFDEVNDAHDVWSLGCVLVRMISGKSAWDEVA 240
           DDEYRKSRRRSSLLKFKGTPRYMSPESIVFDEVNDAHDVWSLGCVLVRMISGKSAWDEVA
Sbjct: 181 DDEYRKSRRRSSLLKFKGTPRYMSPESIVFDEVNDAHDVWSLGCVLVRMISGKSAWDEVA 240

Query: 241 TSKQLMMKILDSKSMPRIPEELSIQGKDFLKKCFVWNFKQRWTADMLLQHPYLKEKHEAT 300
           TSKQLMMKILDSKSMPRIPEELSIQGKDF+KKCFVWNFKQRWTADMLLQHPYL EKHEAT
Sbjct: 241 TSKQLMMKILDSKSMPRIPEELSIQGKDFIKKCFVWNFKQRWTADMLLQHPYLNEKHEAT 300

Query: 301 TMKDDDGMPKLAKSLLLSHQPLKISQTRSSSDHDRKSNLREKNYLSPILRRIKSRK 357
           TMKDDDGMPKLAKSLLLSHQPLKISQTRSSSDHDRKSNLREKNYLSPILRRIKSRK
Sbjct: 301 TMKDDDGMPKLAKSLLLSHQPLKISQTRSSSDHDRKSNLREKNYLSPILRRIKSRK 354

BLAST of CSPI07G05900 vs. NCBI nr
Match: XP_008455530.1 (PREDICTED: mitogen-activated protein kinase kinase kinase A-like [Cucumis melo] >KAA0031448.1 mitogen-activated protein kinase kinase kinase A-like [Cucumis melo var. makuwa] >TYK06901.1 mitogen-activated protein kinase kinase kinase A-like [Cucumis melo var. makuwa])

HSP 1 Score: 510.8 bits (1314), Expect = 9.8e-141
Identity = 274/363 (75.48%), Postives = 304/363 (83.75%), Query Frame = 0

Query: 1   MENWVLVKILGQGSDGLVCLAKQTTRKKIYNNLHYYFAVKLSPLGHNSS-LWEELVLKQF 60
           ME WV VK+LGQGSDG+V LAKQT R+KI  NLHYYFAVKLSPL HNSS LWEE VLKQF
Sbjct: 1   MEKWVFVKVLGQGSDGVVWLAKQTIRRKIGRNLHYYFAVKLSPLRHNSSLLWEEQVLKQF 60

Query: 61  KNCPEIVQYLGSEITRSPGGRDLPGDKDFYTLKLEYAAGGTLDDLIKQKEKLPEDEVKEY 120
           KNCPEIVQYLGSEITR   G DLP DKDFYTLKLEYAAGGTLDDLIKQKEKLPE+EVK+Y
Sbjct: 61  KNCPEIVQYLGSEITR---GGDLPQDKDFYTLKLEYAAGGTLDDLIKQKEKLPENEVKDY 120

Query: 121 LRMILKGLSCIHSKGFVHVDLKPKNILAFPRSNGKMKLKIADFGHAERCK-YRNDADADA 180
           LRMILKGLSC+HSKGFVHVDLKP NILAFP+S+GKMKLKIADFGHAERCK YRN    D 
Sbjct: 121 LRMILKGLSCVHSKGFVHVDLKPNNILAFPQSDGKMKLKIADFGHAERCKLYRN----DD 180

Query: 181 DADDEYRKSRRRSSLLKFKGTPRYMSPESIVFDEVNDAHDVWSLGCVLVRMISGKSAWDE 240
           D DD+ RK     S LKFKGTPRYMSPESIV +EVNDAHD+WSLGCVLV+MISGKS WD+
Sbjct: 181 DDDDQDRKYGGYCSSLKFKGTPRYMSPESIVCNEVNDAHDIWSLGCVLVKMISGKSVWDD 240

Query: 241 VATSKQLMMKILDSK--SMPRIPEELSIQGKDFLKKCFVWNFKQRWTADMLLQHPYL--K 300
           +  S+QLMM++LD+K  +MP IP+ELS QGKDF+KKCF+ N KQRWTADMLLQHPYL  +
Sbjct: 241 LIDSRQLMMRLLDNKNYNMPTIPKELSKQGKDFIKKCFIRNCKQRWTADMLLQHPYLMHE 300

Query: 301 EKHEATTMKDDDGMP-KLAKSLLLSHQ-PLKISQTRSS---SDHDRKSNLREKNYLSPIL 353
           E   ATT+KDDD MP  LAK+L LS Q PLK SQTRSS   ++++RK NLR KNYL P+L
Sbjct: 301 ENEAATTVKDDDQMPTTLAKALSLSRQRPLK-SQTRSSNNNNNNNRKFNLRAKNYLLPLL 355

BLAST of CSPI07G05900 vs. NCBI nr
Match: KAA0059705.1 (mitogen-activated protein kinase kinase kinase ANP1-like [Cucumis melo var. makuwa] >TYK26143.1 mitogen-activated protein kinase kinase kinase ANP1-like [Cucumis melo var. makuwa])

HSP 1 Score: 442.2 bits (1136), Expect = 4.3e-120
Identity = 227/317 (71.61%), Postives = 261/317 (82.33%), Query Frame = 0

Query: 1   MENWVLVKILGQGSDGLVCLAKQTT--RKKIYNNLHYYFAVKLSPLGHNSS-LWEELVLK 60
           MENWV VK+LGQGS G+VCLAKQ++  RKK  +NL YYFAVKLSPL HNSS LWEE VLK
Sbjct: 1   MENWVPVKVLGQGSYGVVCLAKQSSAIRKKSDSNLPYYFAVKLSPLQHNSSLLWEEQVLK 60

Query: 61  QFKNCPEIVQYLGSEITRSPGGRDLPGDK-DFYTLKLEYAAGGTLDDLIKQKEKLPEDEV 120
           QFK+CPEIVQYLGSEITR   GRD+  D  DFYTLKLEYAAGGTLDDLI+Q++KLPE EV
Sbjct: 61  QFKDCPEIVQYLGSEITR---GRDIRNDHVDFYTLKLEYAAGGTLDDLIEQRDKLPEAEV 120

Query: 121 KEYLRMILKGLSCIHSKGFVHVDLKPKNILAFPRSNGKMKLKIADFGHAERCKYRNDADA 180
           K+YLRMILKGLSCIHSKGFVHVDLKP NILAFP+S+GKMKLKIADFG AER KYR     
Sbjct: 121 KDYLRMILKGLSCIHSKGFVHVDLKPNNILAFPQSDGKMKLKIADFGQAERSKYR----- 180

Query: 181 DADADDEYRKSRRRSSLLKFKGTPRYMSPESIVFDEVNDAHDVWSLGCVLVRMISGKSAW 240
               DD++++     S  KFKGTPRYMSPES+VF+EVNDA D+WSLGC+LV+MISGKSAW
Sbjct: 181 ---VDDQHKQYPPCCSSFKFKGTPRYMSPESVVFNEVNDADDIWSLGCILVKMISGKSAW 240

Query: 241 DEVATSKQLMMKILDSKSMPRIPEELSIQGKDFLKKCFVWNFKQRWTADMLLQHPYLKEK 300
           D+   SKQLM+K+LD+K MP IP ELS QGKDF+KKCF+ N+K+RWTA+MLLQHPYL E+
Sbjct: 241 DDCKDSKQLMIKLLDNKIMPTIPGELSEQGKDFIKKCFIRNYKERWTAEMLLQHPYLNEE 300

Query: 301 HEA-------TTMKDDD 307
           +EA        TMK D+
Sbjct: 301 NEAPMNNKNEATMKKDN 306

BLAST of CSPI07G05900 vs. NCBI nr
Match: XP_008465601.1 (PREDICTED: mitogen-activated protein kinase kinase kinase ANP1-like, partial [Cucumis melo])

HSP 1 Score: 441.8 bits (1135), Expect = 5.6e-120
Identity = 227/317 (71.61%), Postives = 260/317 (82.02%), Query Frame = 0

Query: 1   MENWVLVKILGQGSDGLVCLAKQTT--RKKIYNNLHYYFAVKLSPLGHNSS-LWEELVLK 60
           MENWV VK+LGQGS  +VCLAKQ++  RKK  NNL YYFAVKLSPL HNSS LWEE VLK
Sbjct: 1   MENWVPVKVLGQGSYAVVCLAKQSSAIRKKSDNNLPYYFAVKLSPLQHNSSLLWEEQVLK 60

Query: 61  QFKNCPEIVQYLGSEITRSPGGRDLPGDK-DFYTLKLEYAAGGTLDDLIKQKEKLPEDEV 120
           QFK+CPEIVQYLGSEITR   GRD+  D  DFYTLKLEYAAGGTLDDLI+Q++KLPE EV
Sbjct: 61  QFKDCPEIVQYLGSEITR---GRDIRNDHVDFYTLKLEYAAGGTLDDLIEQRDKLPEAEV 120

Query: 121 KEYLRMILKGLSCIHSKGFVHVDLKPKNILAFPRSNGKMKLKIADFGHAERCKYRNDADA 180
           K+YLRMILKGLSCIHSKGFVHVDLKP NILAFP+S+GKMKLKIADFG AER KYR     
Sbjct: 121 KDYLRMILKGLSCIHSKGFVHVDLKPNNILAFPQSDGKMKLKIADFGQAERSKYR----- 180

Query: 181 DADADDEYRKSRRRSSLLKFKGTPRYMSPESIVFDEVNDAHDVWSLGCVLVRMISGKSAW 240
               DD++++     S  KFKGTPRYMSPES+VF+EVNDA D+WSLGC+LV+MISGKSAW
Sbjct: 181 ---VDDQHKQYPPCCSSFKFKGTPRYMSPESVVFNEVNDADDIWSLGCILVKMISGKSAW 240

Query: 241 DEVATSKQLMMKILDSKSMPRIPEELSIQGKDFLKKCFVWNFKQRWTADMLLQHPYLKEK 300
           D+   SKQLM+K+LD+K MP IP ELS QGKDF+KKCF+ N+K+RWTA+MLLQHPYL E+
Sbjct: 241 DDCKDSKQLMIKLLDNKIMPTIPGELSEQGKDFIKKCFIRNYKERWTAEMLLQHPYLNEE 300

Query: 301 HEA-------TTMKDDD 307
           +EA        TMK D+
Sbjct: 301 NEAPMNNKNEATMKKDN 306

BLAST of CSPI07G05900 vs. NCBI nr
Match: XP_031745125.1 (mitogen-activated protein kinase kinase kinase 17 [Cucumis sativus] >KGN44756.1 hypothetical protein Csa_016572 [Cucumis sativus])

HSP 1 Score: 438.0 bits (1125), Expect = 8.1e-119
Identity = 220/313 (70.29%), Postives = 252/313 (80.51%), Query Frame = 0

Query: 1   MENWVLVKILGQGSDGLVCLAKQTTRKKIYNNLHYYFAVKLSPLGHNSS-LWEELVLKQF 60
           MENWV VK+LGQGS  +VCLAKQ+ RK   NNL YYFA+K+ PL HNSS LWEE VLKQF
Sbjct: 1   MENWVPVKVLGQGSYAVVCLAKQSIRKCSDNNLPYYFALKIYPLQHNSSLLWEEQVLKQF 60

Query: 61  KNCPEIVQYLGSEITRSPGGRDLPGDKDFYTLKLEYAAGGTLDDLIKQKEKLPEDEVKEY 120
           K CPEIVQY GSEITR   G     DKDFYTLKLEYAAGGTLDDLIKQ++KLPEDEVK+Y
Sbjct: 61  KGCPEIVQYFGSEITR---GGSFCNDKDFYTLKLEYAAGGTLDDLIKQRDKLPEDEVKDY 120

Query: 121 LRMILKGLSCIHSKGFVHVDLKPKNILAFPRSNGKMKLKIADFGHAERCKYRNDADADAD 180
           LRMILKGLSCIHSKGFVHVDLKP NILAFP+S+GKMKLKIADFG AERCKYR+       
Sbjct: 121 LRMILKGLSCIHSKGFVHVDLKPNNILAFPQSDGKMKLKIADFGQAERCKYRD------- 180

Query: 181 ADDEYRKSRRRSSLLKFKGTPRYMSPESIVFDEVNDAHDVWSLGCVLVRMISGKSAWDEV 240
            D+   K     S LKFKG+PRYMSPESI+F+EV+DAHD+WSLGC+LV+MISGK  WD  
Sbjct: 181 -DNGQHKRYGYCSSLKFKGSPRYMSPESIIFNEVDDAHDIWSLGCILVKMISGKCVWDGY 240

Query: 241 ATSKQLMMKILDSKSMPRIPEELSIQGKDFLKKCFVWNFKQRWTADMLLQHPYLKEKHEA 300
             SKQLM+++LD+K M  IP ELS QGKDF++KCF+ ++KQRWTADMLLQHPYL +++EA
Sbjct: 241 TDSKQLMIEVLDNKIMATIPGELSEQGKDFIRKCFIRSYKQRWTADMLLQHPYLNQENEA 300

Query: 301 ------TTMKDDD 307
                  TMKDD+
Sbjct: 301 PMKEDEATMKDDE 302

BLAST of CSPI07G05900 vs. TAIR 10
Match: AT4G36950.1 (mitogen-activated protein kinase kinase kinase 21 )

HSP 1 Score: 183.3 bits (464), Expect = 3.3e-46
Identity = 118/302 (39.07%), Postives = 161/302 (53.31%), Query Frame = 0

Query: 4   WVLVKILGQGSDGLVCLAKQTTRKKIYNNLHYYFAVKLSPLGHNSSLWEEL-VLKQFKNC 63
           W+  + +G GS   V LA  TT            AVK S +  +++L  E  VL    +C
Sbjct: 3   WIRRETIGHGSFSTVSLA--TTSGSSSKAFPSLMAVKSSGVVCSAALRNERDVLDDLGDC 62

Query: 64  PEIVQYLGSEITRSPGGRDLPGDKDFYTLKLEYAAGGTLDDLIKQK-EKLPEDEVKEYLR 123
            EIV+  G        GR +   ++ Y L LEYA+GG+L D IK   E LPE EV+ + R
Sbjct: 63  SEIVRCFGE-------GRTVENGEEIYNLFLEYASGGSLADRIKSSGEALPEFEVRRFTR 122

Query: 124 MILKGLSCIHSKGFVHVDLKPKNILAFPRSNGKMKLKIADFGHAERCKYRNDADADADAD 183
            I+KGL  IH  GF H D+K +N+L F    G   +KI+DFG A+R              
Sbjct: 123 SIVKGLCHIHGNGFTHCDIKLENVLVF----GDGDVKISDFGLAKR-------------- 182

Query: 184 DEYRKSRRRSSLLKFKGTPRYMSPESIVFDEVNDAHDVWSLGCVLVRMISGKSAW---DE 243
                 R     ++ +GTP YM+PES+   E     D+W+LGC +V M SGK+AW   D 
Sbjct: 183 ------RSGEVCVEIRGTPLYMAPESVNHGEFESPADIWALGCSVVEMSSGKTAWCLEDG 242

Query: 244 VATS-KQLMMKILDSKSMPRIPEELSIQGKDFLKKCFVWNFKQRWTADMLLQHPYLKEKH 300
           V  +   L+++I     +PRIP ELS +GKDF+ KCFV N  +RWTA+MLL HP+L    
Sbjct: 243 VMNNVMSLLVRIGSGDEVPRIPVELSEEGKDFVSKCFVKNAAERWTAEMLLDHPFLAVDD 271

BLAST of CSPI07G05900 vs. TAIR 10
Match: AT4G26890.1 (mitogen-activated protein kinase kinase kinase 16 )

HSP 1 Score: 172.9 bits (437), Expect = 4.5e-43
Identity = 110/300 (36.67%), Postives = 153/300 (51.00%), Query Frame = 0

Query: 3   NWVLVKILGQGSDGLVCLAKQTTRKKIYNNLHYYFAVKLSPLGHNSSLWEELVLKQFKNC 62
           NW    I+G+GS   V +A  ++ +         FAVK + L  +S L +E  +    + 
Sbjct: 4   NWTRGPIIGRGSTATVSIAISSSGE--------LFAVKSADLSSSSLLQKEQSILSTLSS 63

Query: 63  PEIVQYLGSEITRSPGGRDLPGDKDFYTLKLEYAAGGTLDDLIKQK-EKLPEDEVKEYLR 122
           P +V+Y+G+ +TR   G         Y + +EY +GG L DLIK    KLPE E++ Y R
Sbjct: 64  PHMVKYIGTGLTRESNGL-------VYNILMEYVSGGNLHDLIKNSGGKLPEPEIRSYTR 123

Query: 123 MILKGLSCIHSKGFVHVDLKPKNILAFPRSNGKMKLKIADFGHAERCKYRNDADADADAD 182
            IL GL  +H +G VH DLK  N+L     NG   LKIAD G A                
Sbjct: 124 QILNGLVYLHERGIVHCDLKSHNVLV--EENG--VLKIADMGCA---------------- 183

Query: 183 DEYRKSRRRSSLLKFKGTPRYMSPESIVFDEVNDAHDVWSLGCVLVRMISGKSAWDEVAT 242
               KS  +S   +F GTP +M+PE    +E     DVW+LGC ++ M++G S W E+  
Sbjct: 184 ----KSVDKS---EFSGTPAFMAPEVARGEEQRFPADVWALGCTMIEMMTGSSPWPELND 243

Query: 243 SKQLMMKILDSKSMPRIPEELSIQGKDFLKKCFVWNFKQRWTADMLLQHPYLKEKHEATT 302
               M KI  S   P IP  +S + KDFLK C   + KQRWT + LL+HP+L +  E+ T
Sbjct: 244 VVAAMYKIGFSGESPAIPAWISDKAKDFLKNCLKEDQKQRWTVEELLKHPFLDDDEESQT 261

BLAST of CSPI07G05900 vs. TAIR 10
Match: AT5G55090.1 (mitogen-activated protein kinase kinase kinase 15 )

HSP 1 Score: 171.0 bits (432), Expect = 1.7e-42
Identity = 106/296 (35.81%), Postives = 151/296 (51.01%), Query Frame = 0

Query: 2   ENWVLVKILGQGSDGLVCLAKQTTRKKIYNNLHYYFAVKLSPLGHNSSLWEELVLKQFKN 61
           +NW+   I+G+GS   V L           N   +FAVK +    ++ L  E  +    +
Sbjct: 4   QNWIRGPIIGRGSTATVSLG--------ITNSGDFFAVKSAEFSSSAFLQREQSILSKLS 63

Query: 62  CPEIVQYLGSEITRSPGGRDLPGDKDFYTLKLEYAAGGTLDDLIKQK-EKLPEDEVKEYL 121
            P IV+Y+GS +T+         DK  Y L +EY +GG+L DLIK    KLPE  ++ Y 
Sbjct: 64  SPYIVKYIGSNVTKE-------NDKLMYNLLMEYVSGGSLHDLIKNSGGKLPEPLIRSYT 123

Query: 122 RMILKGLSCIHSKGFVHVDLKPKNILAFPRSNGKMKLKIADFGHAERCKYRNDADADADA 181
           R ILKGL  +H +G VH D+K +N++      G    KI D G A               
Sbjct: 124 RQILKGLMYLHDQGIVHCDVKSQNVMI-----GGEIAKIVDLGCA--------------- 183

Query: 182 DDEYRKSRRRSSLLKFKGTPRYMSPESIVFDEVNDAHDVWSLGCVLVRMISGKSAWDEVA 241
                K+   +  L+F GTP +MSPE    +E +   DVW+LGC ++ M +G S W E+ 
Sbjct: 184 -----KTVEENENLEFSGTPAFMSPEVARGEEQSFPADVWALGCTVIEMATGSSPWPELN 243

Query: 242 TSKQLMMKILDSKSMPRIPEELSIQGKDFLKKCFVWNFKQRWTADMLLQHPYLKEK 297
                + KI  +   P IP  LS +G+DFL+KC   + KQRWT + LLQHP+L E+
Sbjct: 244 DVVAAIYKIGFTGESPVIPVWLSEKGQDFLRKCLRKDPKQRWTVEELLQHPFLDEE 259

BLAST of CSPI07G05900 vs. TAIR 10
Match: AT3G50310.1 (mitogen-activated protein kinase kinase kinase 20 )

HSP 1 Score: 167.5 bits (423), Expect = 1.9e-41
Identity = 118/315 (37.46%), Postives = 171/315 (54.29%), Query Frame = 0

Query: 4   WVLVKILGQGSDGLVCLAKQTTRKKIYNNLHYYFAVKLSPLGHNSSLW-EELVLKQFKNC 63
           WV  + +G G+   V  A   T+ +   +     AVK +     +SL  E+ VL    +C
Sbjct: 3   WVRGETIGFGTFSTVSTA---TKSRNSGDFPALIAVKSTDAYGAASLSNEKSVLDSLGDC 62

Query: 64  PEIVQYLGSEITRSPGGRDLPGDKDFYTLKLEYAAGGTLDDLIKQ--KEKLPEDEVKEYL 123
           PEI++  G + T   G       ++ + L LEYA+ G+L   +K+   E LPE  V+ + 
Sbjct: 63  PEIIRCYGEDSTVENG-------EEMHNLLLEYASRGSLASYMKKLGGEGLPESTVRRHT 122

Query: 124 RMILKGLSCIHSKGFVHVDLKPKNILAFPRSNGKMKLKIADFGHAERCKYRNDADADADA 183
             +L+GL  IH+KGF H D+K  NIL F  ++G   +KIADFG A R       D D  A
Sbjct: 123 GSVLRGLRHIHAKGFAHCDIKLANILLF--NDG--SVKIADFGLAMR------VDGDLTA 182

Query: 184 DDEYRKSRRRSSLLKFKGTPRYMSPESIVFDEVNDAHDVWSLGCVLVRMISGKSAWD--E 243
               RKS      ++ +GTP YM+PE +  +E   A DVW+LGC +V M SGK+AW   E
Sbjct: 183 ---LRKS------VEIRGTPLYMAPECVNDNEYGSAADVWALGCAVVEMFSGKTAWSVKE 242

Query: 244 VATSKQLMMKILDSKSMPRIPEELSIQGKDFLKKCFVWNFKQRWTADMLLQHPY----LK 303
            +    L+++I     +P+IPE LS +GKDFL KCFV +  +RWTA+MLL H +    L+
Sbjct: 243 GSHFMSLLIRIGVGDELPKIPEMLSEEGKDFLSKCFVKDPAKRWTAEMLLNHSFVTIDLE 288

Query: 304 EKHE---ATTMKDDD 307
           + H       +KD+D
Sbjct: 303 DDHRENFVVKVKDED 288

BLAST of CSPI07G05900 vs. TAIR 10
Match: AT5G67080.1 (mitogen-activated protein kinase kinase kinase 19 )

HSP 1 Score: 165.6 bits (418), Expect = 7.2e-41
Identity = 115/319 (36.05%), Postives = 165/319 (51.72%), Query Frame = 0

Query: 4   WVLVKILGQGSDGLVCLAKQTTRKKIYNNLHYYFAVK-LSPLGHNSSLWEELVLKQF-KN 63
           W+  + +G G+   V LA ++             AVK     G  S   E+ VL     +
Sbjct: 3   WIRGETIGYGTFSTVSLATRSNNDS--GEFPPLMAVKSADSYGAASLANEKSVLDNLGDD 62

Query: 64  CPEIVQYLGSEITRSPGGRDLPGDKDFYTLKLEYAAGGTLDDLIKQ--KEKLPEDEVKEY 123
           C EIV+  G +       R +   ++ + L LEYA+ G+L+  +K+   E +PE  V+ +
Sbjct: 63  CNEIVRCFGED-------RTVENGEEMHNLFLEYASRGSLESYLKKLAGEGVPESTVRRH 122

Query: 124 LRMILKGLSCIHSKGFVHVDLKPKNILAFPRSNGKMKLKIADFGHAERCKYRNDADADAD 183
              +L+GL  IH+ GF H DLK  NIL F    G   +KIADFG A+R         D  
Sbjct: 123 TGSVLRGLRHIHANGFAHCDLKLGNILLF----GDGAVKIADFGLAKRI-------GDLT 182

Query: 184 ADDEYRKSRRRSSLLKFKGTPRYMSPESIVFDEVNDAHDVWSLGCVLVRMISGKSAWD-- 243
           A          +  ++ +GTP YM+PES+  +E     DVW+LGCV+V M SGK+AW   
Sbjct: 183 A---------LNYGVQIRGTPLYMAPESVNDNEYGSEGDVWALGCVVVEMFSGKTAWSLK 242

Query: 244 EVATSKQLMMKILDSKSMPRIPEELSIQGKDFLKKCFVWNFKQRWTADMLLQHPY----- 303
           E +    L+++I     +P IPEELS QG+DFL KCFV + K+RWTA+MLL HP+     
Sbjct: 243 EGSNFMSLLLRIGVGDEVPMIPEELSEQGRDFLSKCFVKDPKKRWTAEMLLNHPFVTVDV 292

Query: 304 -----LKEKHEATTMKDDD 307
                +KE+     MK +D
Sbjct: 303 DHDVLVKEEDFVVNMKTED 292

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SND62.7e-4037.46Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=370... [more]
O808882.3e-3131.65Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=370... [more]
O220401.9e-3028.44Mitogen-activated protein kinase kinase kinase ANP1 OS=Arabidopsis thaliana OX=3... [more]
F4HRJ44.7e-2933.73Mitogen-activated protein kinase kinase kinase 3 OS=Arabidopsis thaliana OX=3702... [more]
Q54R821.4e-2827.51Mitogen-activated protein kinase kinase kinase A OS=Dictyostelium discoideum OX=... [more]
Match NameE-valueIdentityDescription
A0A0A0K7G35.3e-20198.31Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G065... [more]
A0A5D3C4R64.7e-14175.48Mitogen-activated protein kinase kinase kinase A-like OS=Cucumis melo var. makuw... [more]
A0A1S3C1U94.7e-14175.48mitogen-activated protein kinase kinase kinase A-like OS=Cucumis melo OX=3656 GN... [more]
A0A5D3DR572.1e-12071.61Mitogen-activated protein kinase kinase kinase ANP1-like OS=Cucumis melo var. ma... [more]
A0A1S3CQQ02.7e-12071.61mitogen-activated protein kinase kinase kinase ANP1-like OS=Cucumis melo OX=3656... [more]
Match NameE-valueIdentityDescription
XP_004137063.11.1e-20098.31mitogen-activated protein kinase kinase kinase 17 [Cucumis sativus] >KGN43756.1 ... [more]
XP_008455530.19.8e-14175.48PREDICTED: mitogen-activated protein kinase kinase kinase A-like [Cucumis melo] ... [more]
KAA0059705.14.3e-12071.61mitogen-activated protein kinase kinase kinase ANP1-like [Cucumis melo var. maku... [more]
XP_008465601.15.6e-12071.61PREDICTED: mitogen-activated protein kinase kinase kinase ANP1-like, partial [Cu... [more]
XP_031745125.18.1e-11970.29mitogen-activated protein kinase kinase kinase 17 [Cucumis sativus] >KGN44756.1 ... [more]
Match NameE-valueIdentityDescription
AT4G36950.13.3e-4639.07mitogen-activated protein kinase kinase kinase 21 [more]
AT4G26890.14.5e-4336.67mitogen-activated protein kinase kinase kinase 16 [more]
AT5G55090.11.7e-4235.81mitogen-activated protein kinase kinase kinase 15 [more]
AT3G50310.11.9e-4137.46mitogen-activated protein kinase kinase kinase 20 [more]
AT5G67080.17.2e-4136.05mitogen-activated protein kinase kinase kinase 19 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 4..293
e-value: 1.4E-53
score: 194.0
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 6..293
e-value: 2.0E-49
score: 168.3
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 4..293
score: 37.544865
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 1..354
e-value: 1.6E-57
score: 197.2
NoneNo IPR availablePIRSRPIRSR038172-1PIRSR038172-1coord: 3..296
e-value: 1.9E-17
score: 60.7
NoneNo IPR availablePIRSRPIRSR630220-2PIRSR630220-2coord: 98..282
e-value: 3.7E-6
score: 23.2
NoneNo IPR availablePIRSRPIRSR037921-1PIRSR037921-1coord: 85..254
e-value: 7.2E-8
score: 29.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 320..344
NoneNo IPR availablePANTHERPTHR48011:SF51PROTEIN KINASE SUPERFAMILY PROTEINcoord: 1..331
NoneNo IPR availablePANTHERPTHR48011CCR4-NOT TRANSCRIPTIONAL COMPLEX SUBUNIT CAF120-RELATEDcoord: 1..331
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 2..311

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI07G05900.1CSPI07G05900.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0016798 hydrolase activity, acting on glycosyl bonds
molecular_function GO:0004672 protein kinase activity